PE47396A1 - BODY WEIGHT MODULATOR, NUCLEIC ACID AND CORRESPONDING PROTEINS AND PREPARATION PROCEDURE - Google Patents

BODY WEIGHT MODULATOR, NUCLEIC ACID AND CORRESPONDING PROTEINS AND PREPARATION PROCEDURE

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Publication number
PE47396A1
PE47396A1 PE1995276599A PE27659995A PE47396A1 PE 47396 A1 PE47396 A1 PE 47396A1 PE 1995276599 A PE1995276599 A PE 1995276599A PE 27659995 A PE27659995 A PE 27659995A PE 47396 A1 PE47396 A1 PE 47396A1
Authority
PE
Peru
Prior art keywords
polypeptide
seq
amino acids
refers
body weight
Prior art date
Application number
PE1995276599A
Other languages
Spanish (es)
Inventor
Jeffrey M Friedman
Yiying Zhang
Ricardo Proenca
Margherita Maffei
Jeffrey L Halaas
Ketan Gajiwala
Stephen K Burley
Original Assignee
Univ Rockefeller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/292,345 external-priority patent/US6001968A/en
Priority claimed from US08/438,431 external-priority patent/US6429290B1/en
Priority claimed from US08/483,211 external-priority patent/US6309853B1/en
Application filed by Univ Rockefeller filed Critical Univ Rockefeller
Publication of PE47396A1 publication Critical patent/PE47396A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/06—Preparations for care of the skin for countering cellulitis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/5759—Products of obesity genes, e.g. leptin, obese (OB), tub, fat
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE REFIERE AL POLIPEPTIDO DE OBESIDAD (OB) QUE TIENE: a) DE 145 AMINOACIDOS A 167 AMINOACIDOS, TAL COMO EL QUE COMPRENDE LA SEQ ID No: 2, 4, 5 o 6; b) VARIANTES ALELICAS O ANALOGOS, TAL COMO EL POLIPEPTIDO OB HUMANO, DONDE UNO O MAS AMINOACIDOS SELECCIONADOS DEL GRUPO QUE CONSTA DE LOS AMINOACIDOS 53, 56, 71 ENTRE OTROS, DE ACUERDO CON LA NUMERACION DE LA SEQ ID No:4, ESTAN SUSTITUIDAS CON OTRO AMINOACIDO, TAL COMO EL AMINOACIDO DIVERGENTE DEL POLIPEPTIDO OB DE RATON, ALANINA ENTRE OTROS; c) FRAGMENTOS DEL POLIPEPTIDO DE OB, TAL COMO LA SEQ ID No:18, SEQ ID No:19, SEQ ID No:20 o SEQ ID No:21, QUE TIENEN LA ACTIVIDAD BIOLOGICA. TAMBIEN SE REFIERE AL METODO DE PREPARACION DEL POLIPEPTIDO OB, QUE COMPRENDE: a) CULTIVAR UNA CELULA TAL COMO UNA BACTERIA O UNA LEVADURA EN CONDICIONES QUE PROPORCIONAN LA EXPRESION DEL POLIPEPTIDO DE OB; b) RECUPERAR EL POLIPEPTIDO DE OB EXPRESADO, ADICIONALMENTE; c) SOMETER A CROMATOGRAFIA EL POLIPEPTIDO DE OB, EN UNA COLUMNA DE QUELACION CON Ni, Y LUEGO A UNA COLUMNA DE INTERCAMBIO DE CATIONES; d) PURIFICAR EL POLIPEPTIDO DE OB MEDIANTE FILTRACION EN GEL. TAMBIEN SE REFIERE AL ANTAGONISTA DEL POLIPEPTIDO OB, AL ANTICUERPO ESPECIFICO PARA UN POLIPEPTIDO DE OB Y SU PROCEDIMIENTO DE PREPARACION. ESTOS COMPUESTOS MEJORAN LA APARIENCIA DEL CUERPO AL REDUCIR O INCREMENTAN EL PESO CORPORALIT REFERS TO THE OBESITY POLYPEPTIDE (OB) THAT HAS: a) FROM 145 AMINO ACIDS TO 167 AMINO ACIDS, SUCH AS THAT INCLUDING SEQ ID No: 2, 4, 5 or 6; b) ALELIC VARIANTS OR ANALOGS, SUCH AS THE HUMAN OB POLYPEPTIDE, WHERE ONE OR MORE SELECTED AMINO ACIDS FROM THE GROUP THAT CONSISTS OF AMINO ACIDS 53, 56, 71 AMONG OTHERS, IN ACCORDANCE WITH THE NUMERATION OF SEQ ID No: 4, ARE SUBSTITUTED WITH ANOTHER AMINO ACID, SUCH AS THE DIVERGENT AMINO ACID OF THE POLYPEPTIDE OB OF MOUSE, ALANINE AMONG OTHERS; c) OB POLYPEPTIDE FRAGMENTS, SUCH AS SEQ ID No: 18, SEQ ID No: 19, SEQ ID No: 20 or SEQ ID No: 21, WHICH HAVE THE BIOLOGICAL ACTIVITY. IT ALSO REFERS TO THE METHOD OF PREPARATION OF THE OB POLYPEPTIDE, WHICH INCLUDES: a) GROWING A CELL SUCH AS A BACTERIA OR YEAST IN CONDITIONS THAT PROVIDE THE EXPRESSION OF THE OB POLYPEPTIDE; b) RECOVER THE OB EXPRESSIVE POLYPEPTIDE, ADDITIONALLY; c) SUBMIT OB'S POLYPEPTIDE TO CHROMATOGRAPHY, IN A CHELATION COLUMN WITH Ni, AND THEN A COLUMN OF EXCHANGE OF CATIONS; d) PURIFY THE OB POLYPEPTIDE THROUGH GEL FILTRATION. IT ALSO REFERS TO THE OB POLYPEPTIDE ANTAGONIST, THE SPECIFIC ANTIBODY FOR AN OB POLYPEPTIDE AND ITS PREPARATION PROCEDURE. THESE COMPOUNDS IMPROVE THE APPEARANCE OF THE BODY BY REDUCING OR INCREASING BODY WEIGHT

PE1995276599A 1994-08-17 1995-08-17 BODY WEIGHT MODULATOR, NUCLEIC ACID AND CORRESPONDING PROTEINS AND PREPARATION PROCEDURE PE47396A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/292,345 US6001968A (en) 1994-08-17 1994-08-17 OB polypeptides, modified forms and compositions
US08/347,563 US5935810A (en) 1994-08-17 1994-11-30 Mammalian ob polypeptides capable of modulating body weight, corresponding nucleic acids, and diagnostic and therapeutic uses thereof
US08/438,431 US6429290B1 (en) 1994-08-17 1995-05-10 OB polypeptides, modified forms and derivatives
US08/483,211 US6309853B1 (en) 1994-08-17 1995-06-07 Modulators of body weight, corresponding nucleic acids and proteins, and diagnostic and therapeutic uses thereof

Publications (1)

Publication Number Publication Date
PE47396A1 true PE47396A1 (en) 1996-12-02

Family

ID=46507740

Family Applications (1)

Application Number Title Priority Date Filing Date
PE1995276599A PE47396A1 (en) 1994-08-17 1995-08-17 BODY WEIGHT MODULATOR, NUCLEIC ACID AND CORRESPONDING PROTEINS AND PREPARATION PROCEDURE

Country Status (5)

Country Link
KR (1) KR100584177B1 (en)
BR (1) BRPI9508596B1 (en)
PE (1) PE47396A1 (en)
RS (1) RS50274B (en)
UA (1) UA70911C2 (en)

Also Published As

Publication number Publication date
BRPI9508596B1 (en) 2018-11-27
KR100584177B1 (en) 2006-10-24
UA70911C2 (en) 2004-11-15
RS50274B (en) 2009-07-15
YU54995A (en) 1998-05-15
KR970705631A (en) 1997-10-09

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