OA20811A - Concentrated algal extract. - Google Patents

Abstract

The present invention relates to a composition of seaweed algal extracts having a dry matter content between 18-36%. These extracts preferably belong to Ascophyllum nodosum. The said extracts are also characterised using bioactive compounds found in them inherently. The present invention also provides a method of making the said composition and characterising the same.

Description

Concentrated Algal Extract

Field of invention

The présent invention relates to a concentrated algal extract having a percentage dry natter between 18,0% to 36,0% or its use to stimulate the gene expression in plants. The présent invention also relates to a concentrated algal extract characterized by the presence of active agents like Fucoidans (6-12g/kg), Glycine Betaine (8-47 mg/Kg)), Laminine (208-335 mg/Kg), and Mannitol (30-60 g/Kg).

Background of invention

The global effect of négative climatic changes are leading to désertification, soil salination, reduced nutrients capacity of soils and like impacts that are causing dramatic effects on agricultural production and quaiity of crops. With increasing population and urbanization, the global amount of cultivable land is also shrinking. in order to meet the food need of fast growing population world food production needs to be doubied by 2050.

To address these issues, high productivity in agriculture is a must that can meet Ί5 the rising demand of food. To achieve this, producers had started relying on the applications of Chemical like synthetic fertilizers or synthetic insecticides or synthetic fungicides or like.

Plant biostimulants are input compounds or compound complexes that offer a potential alternative to traditional, agro-chemical inputs.

According to the European Biostimulants Industry Council (EBIC), “plant biostimulants contain substance(s) and/or microorganisms whose function when applied to plants or the rhizosphere is to stimulate natural processes to enhance/benefit nutrient uptake, nutrient efficîency, tolérance to abiotic stresses, and crop quaiity”. Algae or seaweed extract are one of the input agents described as biostimulant by the EBIC,

Seaweeds are multicellular, macroscopic organisms found in Coastal, marine ecosystems and possess high quantity of polysaccharides, polyunsaturated fatty acids (PUFA), enzymes, minerais, bioactive enzymes and other components. These extracts are known to enable better seed germination, espécial!y by promoting plant emergence. The most widely researched seaweed is Ascophyllum nodosum and various commercial products are available from the extracts of 5 Ascophyllum nodosum. These extracts hâve been reported to improve the plant growth.

US20170251677A1 mentions about a concentrated extract of Ascophyllum nodosum, and its use as biostimulant alone or in combination with other agents. The référencé specifically teaches an algal extract having dry matter of 5% or 13%.

The article ‘Effect of different concentrations of commercial seaweed Iiquid extract of Ascophyllum nodosum (Organic Dews) as a plant bio stimulant on growth, yield and biochemical constituents of onion (Allium cepa L.) by Akash et al., J. Pharmacognosy and Phytochemistry, 2017’ studies the effect of different concentrations of seaweed Iiquid extract of Ascophyllum nodosum as biostimulant. The article further concludes that as the concentration of the extract is increased 15 its impact decreases.

However, there still exists a need in the art to provide the best formulations of these extracts which are concentrated and yet effective. Since the extract of Ascophyllum nodosum is a complex of various biological and biochemical ingrédients, its best quality and optimum use is still a pondering question, Further, the dîluted extracts 20 are meant to be appiied at higher dose thus încreasing the cost of farmers. Thus, there lies a need for a concentrated algal extract that has good biostimulant activity and is also cost effective.

Objects of the invention

An object of the présent invention is to provide an algal extract of Ascophyllum 25 nodosum having maximum efficacy.

it is an object of the présent invention to provide an algal extract of Ascophyllum nodosum having a characteristic active agents distribution profile and the link of these markers with efficiency.

Yet another object of this invention is to provide a process for preparing an algal 5 extract of Ascophyllum nodosum having a predetermined dry matter content.

Yet another object of this invention is to provide a process for preparing an algal extract of Ascophyllum nodosum having a characteristic active agents distribution profile leading to an improved efficacy.

Yet another object of this invention is to provide a use of seaweed extract of 10 Ascophyllum nodosum for biostimulating the plant growth.

Yet another object of this invention is to provide a use of seaweed extract of Ascophyllum nodosum as bird repellant.

Yet another object of this invention is to provide a use of seaweed extract of Ascophyllum nodosum as a seed coating agent.

Yet another object of this invention is to provide a combination comprising an algal extract of Ascophyllum nodosum with nutritive, biostimulating or another agrochemical.

Summary of the invention

In an aspect the present invention provides an algal extract of Ascophyllum nodosum with dry matter between about 18%-36%.

In an aspect the present invention provides an algal extract of Ascophyllum nodosum with dry matter between about 24%-36%.

In an aspect the present invention provides an algal extract of Ascophyllum nodosum with dry matter between about 24%-30%.

In another aspect, the présent invention provides an algal extract of Ascophyllum nodosum characterized by the concentrations of active agents Fucoidans being about 6-15 g/kg, Glycine Betaine being about 8-47 mg/Kg, Laminine being about 208- 438 mg/Kg, and Mannitoi being about 30-60 g/Kg by fresh weight of the algal 5 extract.

in another aspect, the présent invention provides a process for préparation of algal extract of Ascophyllum nodosum with dry matter of 18%-36%.

In an aspect the présent invention provides a process for préparation of algal extract of Ascophyllum nodosum characterized by different active agents fucoidans 10 being about 6-12g/kg, glycine betaine being about 8-47 mg/Kg, laminine being about 208-335 mg/Kg, and mannitoi being about 30-60 g/Kg, by weight of the algal extract.

In an aspect the present invention provides the use of seaweed extract of Ascophyllum nodosum for biostimulating the plant growth.

In an aspect the present invention provides the use of seaweed extract of Ascophyllum nodosum as bird repellant.

In an aspect the présent invention provides the use of seaweed extract of Ascophyllum nodosum as seed coating agent.

In an aspect the present invention provides the use of seaweed extract of 20 Ascophyllum nodosum as animai nutrition supplément.

In an aspect the present invention provides the use of seaweed extract of Ascophyllum nodosum as cosmetic agent.

In an aspect the present invention provides the use of seaweed extract of Ascophyllum nodosum in medicines or nutraceuticals.

In an aspect the présent invention provides a combination of algal extract of Ascophyllum nodosum with nutritive, biostimuîating agent or plant-protecting composition, where the plant protecting composition is herbicide, fungicide, insecticide or pesticide.

In an aspect the présent invention provides a combination of algal extract of Ascophyllum nodosum with a super absorbent polymer.

Detailed description ofthe invention

The présent inventors hâve found that an algal extract of Ascophyllum nodosum that is highly concentrated and yet has ail the compounds of the intracellular fluid 10 of the alga preserved has excellent biostimuîating activity. Surprisingly, the inventors hâve observed that, foliowing application in very less amount of the concentrated aigal extract of the invention is more effective than a diluted extract in stimulating plant emergence and promoting plant growth. It was also observed that the different components of the extract were responsible for biostimuîating 15 spécifie gene expressions in the plants.

Also, when the concentrated algal extract is used to coat seeds, the latter are less frequentiy attacked by birds than uncoated seeds.

For convenience, before further description of the présent disclosure, certain terms employed in the spécification, and examples are described here. These définitions 20 should be read in the light of the remainder of the disclosure and understood as by a person of skill in the art. The terms used herein hâve the meanings recognized and known to those of skill in the art. However, for convenience and completeness, particular terms and their meanings are set forth below.

As used herein the term 'plant’ refers to an agricultural plant having sugar content 25 as agronom ica lly important trait for example wheat, rye, bariey, rice, triticale, oats, sorghum, sugarcane, , beet, sugar beet or fodder beet, fruits like pomes, apples, pears, plums, peaches, almonds, cherries, strawberries, raspberries, blackberries or gooseberries, leguminous plants, such as lentils, peas, alfalfa or soybeans, oil plants, such as râpe, oil-seed râpe, canola, juncea, linseed, mustard, olives, sunflowers, cocon ut, cocoa beans, castor oil plants, oil palms, ground nuts or soybeans, cucurbits, such as squashes, cucumber or melons, fiber plants, such as cotton, flax, hemp or jute, citrus fruit, such as oranges, lemons, grapefruits or 5 mandarins, vegetables, such as spinach, îettuce, asparagus, cabbages, carrots, onions, tomatoes, cucurbits or paprika, lauraceous plants, such as avocados, cinnamon or camphor, energy and raw material plants, such as corn, soybean, râpe, canola, oil palm, corn, tobacco, nuis, coffee, tea, bananas, vines, hop, turf, naturel rubber plants or ornamental and forestry plants, such as flowers and 10 shrubs.

As used herein seaweed refers to the coldwater seaweed or brown alga (Phaeophyceae) of the family Fucaceae, belonging to the genus Ascophyilum.

Active agents are the agents that are présent in the algal extract obtained by varions processes of algal extraction, The algal extract may contain one or more 15 than one active compound selected from, but not limited to polysaccharides, such as, especially, laminarin and fucans; free and conjugated sugars; polyphenols; mannitol; growth hormones; lipids; proteins; amino acids; vitamine; betaines; sterols; glucuronic acid and minerai salts.

Alternative Boron source is any Boron source that is less toxic or non-toxic than 20 the conventional boron source, It can be selected from but not limited to Colemanite.

The term “cultivation” as used herein refers to an activîty to grow plants in any stage from the seeding stage to maturation stage. It means to artificially grow plants over the entire or in partial period from the seeding stage to the maturation stage and in each following stage or in stages by the combination of two or more 25 of the stages such as (1) from nursery stage to maturation stage; (2) from nursery plants to maturation stage; (3) from seeds to nursery plants; (4) from the stage between before the desired maturation to the desired maturation and (5) from the nursery plants to the stage before the desired maturation.

The terms “superabsorbent polymer” or “SAP” referred herein refer to the water swellable polymers which can absorb water many times their weight in an aqueous solution, Without wishing to be bound by theory, the term superabsorbent polymers also appiy to polymers that absorb water as well as de-sorb the absorbed water.

The superabsorbent polymer may be selected from but not limited to waterswellable or water absorbing or water-retentive polymers such as cross-linked polymers that swell without dissolving in the presence of water, and may, absorb at least 10, 100, 1000, or more times their weight in water.

As used herein the plant protective, nutrient and surfactant adjuvant composition 10 is selected from but not limited to fertilizers, mycorrhiza, micronutrients, acaricides, algicides, antifeedants, avicides, bactéricides, bird repellents, chemosterilants, fungicides, herbicide safeners, herbicides, insect attractants, insect repellents, insecticides, mammal repellents, mating disruptors, molluscicides, nematicides, plant activators, plant-growth regulators, rodenticides, synergists, virucides, 15 dérivatives thereof, biological control agents and mixtures thereof.

As used here in the gene expression describes the upregulation or downrégulation of gene expression in the organism.

In an embodiment, the fertilizer is selected from organic and inorganic fertilizers such as those selected from but not limited to urea, NPK, nitrogen based fertilizers, 20 phosphate, calcium, potassium, magnésium, sulfur, copper, iron, manganèse, molybdenum, zinc, nickel, cobalt, boron and their salts and dérivatives.

In an embodiment, the mîcronutrient is selected from but not limited to Iran, manganèse, boron, molybdenum, zinc, chlorine, sodium, cobalt, Silicon, nickel, chlorine, aluminium, vanadium, sélénium and their salis and dérivatives.

Exemplary organic fertilizer may be selected from peat, limestone, rock phosphate, blood meal, bone meal, compost, humic acid, seaweed extracts, digested proteins, fish meal, feather meal, corn meal, alfalfa meal etc.

In an embodiment, the insecticide may be selected from organic or inorganic insecticide, natural or synthetic insecticide such as those selected from but not limited to: Acétylcholine esterase inhibitors (Carbamates, Triazemate, Organophosphates); GABA-gated chloride channel antagoniste (Cyclodiene 5 organochlorines, Phenylpyrazoles (Fiproles)); Nicotinic Acétylcholine receptor agonists (allosteric) (Spinosyns); Chloride channel activators (Avermectins, Milbemycins); Inhibitors of chitin biosynthesis, type 0, Lepidopteran (Benzoylureas ); Inhibitors of chitin biosynthesis, type 1, Homopteran (Buprofezin); Moulting disruptor, Dipteran (Cyromazine ); Ecdysone agonists l moulting disruptors 10 (Diacylhydrazines, Azadirachtin); Octopaminergic agonists (Amitraz); Neuronal inhibitors (unknown mode of action) (Bifenazate ); Aconitase inhibitors (Fluoroacetate); Synergists (P450-dependent monooxygenase inhibitors, Esterase inhibitors); Ryanodine receptor modulators (diamide); Compounds with unknown mode of action (Benzoximate, Chinomethionat, Dicofol, Pyridalyl, Borax, 15 Tartar emetic); Compounds of unknown or non-specific mode of action (fumigants) (Alkyl halides, Chloropicrin, Sulfuryl fluoride); Sodium channel modulators (DÛT, Methoxychlor, Pyrethroids, Pyrethrins); Microbial disruptors of insect midgut membranes (B.t. subsp. Israelensis, B. sphaericus, B.t. subsp. Aizawai, B.t. subsp. kurstaki, B.t. subsp. tenebrionis); Mitochondrial complex IV électron transport 20 inhibitors (Aluminium phosphide, Cyanide, Phosphine); Mitochondrial complex III électron transport inhibitors (Coupling site II) (Hydramethylnon, Acequinocyl, Fluacrypyrim); Compounds of unknown or non-specific mode of action (Clofentezine, Hexythiazox, Etoxazole); Nicotinic Acétylcholine receptor agonists / antagonists (Neonicotinoids, Nicotine, Bensultap, Cartap hydrochloride, 25 Nereistoxin analogues); Juvénile hormone mimics (Juvénile hormone analogues, Fenoxycarb, Pyriproxyfen); Inhibitors of oxidative phosphorylation, disruptors of ATP formation (inhibitors of ATP synthase) (Diafenthiuron, Organotin miticides, Propargite, Tetradifon); Mitochondrial complex I eiectron transport inhibitors (METI acaricides, Rotenone); Voltage-dependent sodium channel blockers (Indoxacarb);

Inhibitors of lipid synthesis (Tetronic acid dérivatives); Mitochondrial complex IV eiectron transport inhibitors (Aluminium phosphide, Cyanide, Phosphine); Compounds of unknown or non-specific mode of action (sélective feeding blockers) (Cryolite, Pymetrozine, Flonicamid); Uncouplers of oxidative phosphorylation via disruption of proton gradient (Chlorfenapyr, DNOC).

Surprîsingly, the présent inventors hâve found that an algal extract of Ascophylium nodosum that is highly concentrated and yet has ail the compounds of the intracellular fluid of the alga preserved and has excellent biostimulating activity. The inventors hâve observed that, following application in very less amount of the concentrated algal extract of the invention is more effective than a diluted extract in stimulating plant emergence and promoting plant growth. It was also observed that the different components of the extract were responsible for biostimulating spécifie gene expressions in the plants.

In an embodiment the présent invention provides an algal extract of Ascophylium 10 nodosum having dry matter between about 18%-36%.

In an embodiment the present invention provides an algal extract of Ascophylium nodosum having dry matter between about 24%-36%.

In an embodiment the present invention provides an algal extract of Ascophylium nodosum having dry matter between about 24%-36%.

In an embodiment the présent invention provides an algal extract of Ascophylium nodosum having dry matter between about 24%-30%.

In an embodiment the present invention provides an algal extract of Ascophylium nodosum having dry matter between about 25%-29%.

In an embodiment the present invention provides an algal extract of Ascophylium 20 nodosum having dry matter between about 26%-28%.

In an embodiment the présent invention provides an algal extract of Ascophylium nodosum having dry matter content of about 27%.

It has been surprîsingly found that an algal extract having percentage dry matter content between about 18% to 36% has surprîsingly higher efficacy.

In an embodiment, the algal extract of the présent invention is characterized by the concentrations of active agents Fucoidans being about 6-15 g/kg, Glycine Betaine being about 8-47 mg/Kg, Laminine being about 208-335 mg/Kg, and Mannitol being about 30-60 g/Kg by weight of the algal extract.

In an embodiment the présent invention provides a process of préparation of algal extract of Ascophyllum nodosum with dry matter of 24%-30%.

In an embodiment the présent invention provides a process of préparation of algal extract of Ascophyllum nodosum characterized by the concentrations of active agents Fucoidans being about 6-15 g/kg, Glycine Betaine being about 8-47 mg/Kg, 10 Laminine being about 208-335 mg/Kg, and Mannitol being about 30-60 g/Kg by weight of the algal extract.

In an embodiment the present invention provides the use of seaweed extract of Ascophyllum nodosum according to the present invention for biostimulating the plant growth.

Ί5 In an embodiment the présent invention provides the use of seaweed extract of Ascophyllum nodosum according to the present invention for overexpressing the phosphorus PT2 gene, and LKT1 gene in leaves.

In an embodiment the present invention provides the use of seaweed extract of Ascophyllum nodosum according to the present invention for increasing the 20 jasmonate isoleucine amount in roots.

In an embodiment the present invention provides the use of seaweed extract of Ascophyllum nodosum as bird repeliant.

In an embodiment the present invention provides the use of seaweed extract of Ascophyllum nodosum as seed coating agent.

In an embodiment the present invention provides the use of seaweed extract of Ascophyllum nodosum as animal nutrition supplément.

In an embodiment the présent invention provides the use of seaweed extract of Ascophyllum nodosum as cosmetic agent.

In an embodiment the présent invention provides the use of seaweed extract of Ascophyllum nodosum in medicines or nutraceuticals.

In an embodiment the présent invention provides a combination of algal extract of Ascophyllum nodosum with nutritive, biostimulating or plant-protecting composition or another agrochemical, where the plant protecting composition is herbicide, fungicide, insecticide or pesticide.

In an embodiment the présent invention provides a combination comprising an 10 algal extract of Ascophyllum nodosum having percentage dry matter content between about 18% to about 36% and at least one superabsorbent polymer.

In an embodiment the présent invention provides a combination comprising an algal extract of Ascophyllum nodosum having percentage dry matter content between about 24% to about 30% and at least one superabsorbent polymer.

In an embodiment, the application of the algal extract of the présent invention to the plants confers one or more of thefollowing advantages: improved plant nutrient use efficiency, increased flowering, increased fruit set quality, facilitâtes root growth etc., which are not to be considered as limiting.

In an embodiment, the algal extract of the présent invention has a pH of around 20 2.25-2.30.

In an embodiment, the mannitol content in the algal extract, measured between one to three months after production, is between about 4% to about 7%.

In an embodiment, the mannitol content in the algal extract, measured between one to three months after production, is between about 4.5 to 6.5%.

In an embodiment, the mannitol content in the algal extract, measured between one to three months after production, is between about 5-6%.

In an embodiment, the algal extract of the présent invention is substantially free of alginate.

in an embodiment, the algal extract according to the invention may be prepared from an algal extract conventionally known from US 2017/0251677 and which includes a percentage dry matter content between 12% to 50%. This algal extract may be used as the starting material for preparîng the algal extract according to the présent invention.

In an embodiment, the présent invention provides a process for preparîng an algal extract having a dry matter content between about 18 - 36%.

In an embodiment, the présent invention provides a process for preparîng an algal extract having a dry matter content between about 24 - 36%.

In an embodiment, the présent invention provides a process for preparîng an algal Ί 5 extract having a dry matter content between about 24 - 30%.

In an embodiment, the process comprises:

(a) feeding an initial algal extract comprising dry matter between 12 - 50% to an evaporator heat exchanger; and (b) passing steam through the heat exchanger to produce the algal extract of 20 the present invention.

In an embodiment, the process comprises:

(a) feeding an initial algal extract comprising dry matter between 3 - 8%to an evaporator heat exchanger; and (b) passing steam through the heat exchanger to produce the algal extract of the present invention.

In an embodiment, the steam passed through the heat exchanger is hot dry steam.

In an embodiment, steam is passed through the heat exchanger maintained at a température between 30 - 60°C.

In an embodiment, steam is passed through the heat exchanger maintained at 5 vacuum pressure.

In an embodiment, the process comprises adding one or more preservatives to the algal extract produced in step (b) ofthe process.

Thus, in an embodiment, the process comprises:

(a) feeding an initial algal extract comprising dry matter between 12 - 50% to an evaporator heat exchanger;

(b) passing steam through the heat exchanger to produce the algal extract of the présent invention; and (c) adding one or more preservatives to the algal extract produced in step (b) of the process.

Thus, in an embodiment, the process comprises:

(a) feeding an initial algal extract comprising dry matter between 3 - 8% to an evaporator heat exchanger;

(b) passing steam through the heat exchanger to produce the algal extract of the présent invention; and (c) adding one or more preservatives to the algal extract produced in step (b) □f the process.

In an embodiment, the process comprises decanting the algal extract produced in step (b) and removing the sédiment produced therefrom to préparé the algal extract according to the present invention.

Thus, in an embodiment, the process comprises:

(a) feeding an initial algal extract comprising dry matier between 12 - 50% to an evaporator heat exchanger;

(b) passing steam through the heat exchanger to produce a concentrated algal extract; and (c) decanting the algal extract produced in step (b) and removing the sédiment produced therefram to préparé the algal extract.

Thus, in an embodiment, the process comprises;

(a) feeding an initial algal extract comprisîng dry matter between 3 - 8% to an evaporator heat exchanger;

(b) passing steam through the heat exchanger to produce a concentrated algal extract; and (d) decanting the algal extract produced in step (b) and removing the sédiment produced therefrom to préparé the algal extract.

Thus, in an embodiment, the process comprises:

(a) feeding an initial algal extract comprisîng dry matter between 12 - 50% to an evaporator heat exchanger;

(b) passing steam through the heat exchanger to produce the algal extract of the present invention;

(c) adding one or more preservatives to the algal extract produced in step (b); and (e) decanting the algal extract produced in step (c) and removing the sédiment produced therefrom to préparé the algal extract

Thus, in an embodiment, the process comprises:

(c) feeding an initial algal extract comprisîng dry matter between 3 - 8% to an evaporator heat exchanger;

(d) passing steam through the heat exchanger to produce the algal extract of the present invention;

(d) adding one or more preservatives to the algal extract produced in step (b); and (f) decanting the algal extract produced in step (c) and removing the sédiment produced therefrom to préparé the algal extract.

However, the step of sédiment removal may be carried out using any other method that is conventionally known.

Thus, in an embodiment, the process comprises:

(a) feeding an initial algal extract comprising dry matter between 3-6% to an evaporator heat exchanger;

(b) passing steam through the heat exchanger to produce a concentrated algal extract; and (c) removing sédiments from the algal extract prepared in step (b) to préparé the algal extract.

Thus, in an embodiment, the process comprises:

(a) feeding an initial algal extract comprising dry matter between 12 - 50% to an evaporator heat exchanger;

(b) passing steam through the heat exchanger to produce the algal extract of the present invention;

(c) adding one or more preservatives to the algal extract produced in step (b); and (d) removing sédiments from the algal extract prepared in step (c) to préparé the algal extract.

In an embodiment, the step of removing the sédiments may be carried out by filtering the algal extract on diatomaceous earth.

In an embodiment, the step of removing the sédiments may be carried out by filtering the algal extract on polymeric membranes.

In an embodiment, the polymeric membrane is polypropylene membrane.

In an embodiment, the step of removing the sédiments may be carried out by centnfuging the algal extract.

In an embodiment, the step of removing the sédiments may be carried out by spray drying the algal extract.

In an embodiment, the step of removing the sédiments may be carried out by adding a thickener or an anti-settling agent to stabilize the sédiment particles.

In an embodiment, the step of removal of sédiments may be carried out by carrying out more than one of the steps of filtration on diatomaceous earth, filtration on a 5 polymeric membrane, centrifugation, spray drying or addition of a thickener simultaneously.

In an embodiment, the present invention provides a process for preparîng a composition comprising the algal extract of the invention.

The process comprises adding ai least one surfactant to the algal extract of the 10 invention prepared by any one of the processes described hereinabove.

In an embodiment, the surfactant is a wetting agent.

In an embodiment, the wetting agent has a contact angle at least greater than 80°.

In an embodiment, the process for preparîng a composition comprises adding at least one sticker to the composition.

It has been found that addition of a sticker increases the rainfastness of the compositions according to the invention.

In an embodiment, the process for preparîng a composition comprises adding at least one adjuvant to the composition.

It has been found that addition of an adjuvant increases the efficacy of the 20 compositions according to the invention.

In an embodiment, the algal extract of the present invention has a Prix index of at least about or greater than 5.0.

In an embodiment, the algal extract of the présent invention has a brix index of at least about or greater than 15,0.

in an embodiment, the algal extract of the present invention has a brix index of at least about or greater than 25.0.

In an embodiment, the algal extract of the present invention has a brix index of at least about 35.0.

In an embodiment, the algal extract of the present invention has a brix index profile as depicted in figure 1.

The algal extract of the present invention may be combined with another substance 10 to provide combinations of the invention.

Therefore, in an embodiment, the present invention provides a combination comprising:

(a) an algal extract having a percentage dry matter content between 18 - 36%; and (b) a superabsorbent polymer.

Therefore, in an embodiment, the present invention provides a combination comprising:

(c) an algal extract having a percentage dry matter content between 24 - 36%; and (d) a superabsorbent polymer.

Therefore, in an embodiment, the present invention provides a combination comprising:

an algal extract having a percentage dry matter content between 24 - 30%; and a superabsorbent polymer.

The choice of the particular superabsorbent polymer is not particularly limiting. Any superabsorbent polymer may be used.

In an embodiment, the superabsorbent polymer is selected from the group consisting of copolymer of acrylamide and sodium acrylate; hydrolyzed starch- polyacrylonitrile; 2- propenenitrile homopolymer, hydrolyzed, sodium sait or poly(acrylamide co-sodium acrylate) or poly(2-propenamide-co-2-propanoic acid, sodium sait); starch-g- poly(2propenamide-co-2-propanoic acid, mixed sodium and aluminum salts); starch-g- poly(2-propenamide-co-2-propanoic acid, potassium sait); poly(2-propenamide-co-2- propanoic acid, sodium sait); poly-2-propanoic acid, sodium sait; starch-gpoly(acryloniirile) or poly(2-propenamide-co-sodium acrylate); starch/acrylonitrile copolymer; crosshnked copolymers of acrylamide and sodium acrylate; acrylamide/sodium polyacrylate crosshnked polymers ; anionic poly acrylamide; starch grafted sodium poîyacrylates; acrylic acid polymers, sodium sait; crosshnked potassium polyacrylate/polyacrylamide copolymers ; sodium poiyacrylate; superabsorbent polymer laminates and composites; pectins; partial sodium sait of crosslînked polypropenoic acid; potassium polyacrylate, lightly crosshnked; sodium polyacrylate, lightly crosslînked; sodium polyacrylates; poly(sodium acrylate) homopolymer;

poiyacrylamide polymers, carrageenan, agar, alginic acid, guar gums and its dérivatives, and gellan gum; Spécifie superabsorbent polymers include crosslînked copolymer of acrylamide and potassium acrylate.

In another embodiment, the algal extract of the invention may be combîned with at least another agrochemical.

Therefore, in an embodiment, the present invention provides a combination 25 comprising:

(a) an algal extract having a percentage dry matter content between 18 - 36%; and (b) at least another agrochemical selected from fertilizers, mycorrhiza, micronutrients, acaricides, algicides, antifeedants, avicides, bactéricides, bird repellents, chemosterilants, fungicides, herbicide safeners, herbicides, insect attractants, insect repellents, insecticides, mammal repellents, mating disruptors, molluscicides, nematicides, plant activators, plantgrowth regulators, rodenticides, synergists, virucides, dérivatives thereof, 5 biological control agents and mixtures thereof.

Therefore, in an embodiment, the présent invention provides a combination comprising:

(c) an algal extract having a percentage dry matter content between 24 - 36%; and (d) at least another agrochemical selected from fertilizers, mycorrhiza, 10 micro nutrients, acaricides, algicîdes, antifeedants, avicides, bactéricides, bird repellents, chemosterilants, fungicides, herbicide safeners, herbicides, insect attractants, insect repellents, insecticides, mammal repellents, mating disruptors, molluscicides, nematicides, plant activators, plantgrowth regulators, rodenticides, synergists, virucides, dérivatives thereof, 1 5 biological control agents and mixtures thereof.

Therefore, in an embodiment, the présent invention provides a combination comprising:

(a) an algal extract having a percentage dry matter content between 24 - 30%; and (b) at least another agrochemical selected from fertilizers, mycorrhiza, 20 micronutrients, acaricides, algicîdes, antifeedants, avicides, bactéricides, bird repellents, chemosterilants, fungicides, herbicide safeners, herbicides, insect attractants, insect repellents, insecticides, mammal repellents, mating disruptors, molluscicides, nematicides, plant activators, plantgrowth regulators, rodenticides, synergists, virucides, dérivatives thereof, 25 biological control agents and mixtures thereof.

In an embodiment, the fertilizer may be selected from organic and inorganic fertilizers such as those selected from but not limited to urea, NPK, nitrogen based fertilizers, phosphate, calcium, potassium, magnésium, sulfur, copper, iron, manganèse, molybdenum, zinc, nickel, cobalt, boron and their salts and 30 dérivatives. Exemplary organicfertilizer may be selected from peat, limestone, rock phosphate, blood meal, bone meal, compost, humic acid, seaweed extracts, digested proteins, fish meal, feather meal, corn meal, alfaifa meal etc.

In a preferred embodiment, the fertilizer may be an inorganic fertilizer selected from NPK, dérivatives of potassium, zinc, phosphate such as those selected from but 5 not limited to potassium fertilizers such as potassium carbonate, potassium chloride (also known as muriate of potash), potassium sulfate, potassium nitrate, sulfate of potash magnesia; zinc fertilizers such as those selected from zinc sulfate, zinc oxide, zinc ammonia complex; phosphate fertilizer such as Di-ammonium phosphate, Monoammonium phosphate, Ammonium polyphosphate, Triple superphosphate or mixtures thereof. In an embodiment, the fertilizer may be an organic fertilizer selected from urea, rock potash seaweed extract, compost, humic acid, alfaifa meal, corn meal, and mixtures thereof. In an embodiment, the present invention may provide a composition comprisîng at least one superabsorbent polymer, at least one water soluble phosphate and a fertilizer mix of organic and 15 inorganic fertilizers.

In an embodiment, the second agrochemical may be a biological control agent.

In a preferred embodiment, the biological control agent may be selected from complété organism or extracts of bacteria or fungî or algae or yeast or combinations thereof.

The composition combines with a micro-biological entity such as bacteria, fungi that is used in isolation or combined with other strains. The strains of bacteria that may be used for the composition inciude but are not limited to: Pseudomonas spp., Bacillus spp., Sînorhizobium spp., Azotobacter spp., Beijerinkia spp., Clostridium spp. , Azollae spp., Nitrosomonas spp. , Nitrobacter spp., Tsukamorella spp.,

Nostoc spp., Enterobacterspp., Thiobacillus spp., Microsporaspp., Beggiato spp., Paracoccus spp., Purple Sulphur Bacteria, Green Sulphur Bacteria, Acîdanus spp. , Sulpholobus, Microbacterium spp., Frateuria spp., Azospirillus spp., Frankia spp., Azospirillium spp., Anabaena spp., Azoilae spp. , Brevibacillus spp., Fusarium spp., Rhizoctonia spp., Pythium spp., Lactobacillus, Ensiferspp., Trichodesmium spp., Spirillium spp. , Klebsiella spp., Mesorhizobium spp., Azorhizobium spp., Acidothiobacillus spp., Paenibacillus spp., Burkholderia spp.,

Alcaligenesacinetobacter spp., Arthrobacter spp., Erwinia spp., Flavobacterium spp., Serratia spp., Herbaspirhlum spp., Achromobacter spp., Aeromonus spp., Phyllobacterium spp., Micrococcus spp., Rhodococcus spp., and combinations thereof.

The strains of fungi that may be used for the composition include but are not limited to.Pantoea spp., Pénicillium spp., Aspergillus spp., Glomus spp., Gigaspora spp., Acaulospora spp., Scutellospora spp., Trichoderma spp., Beauveria spp., Mychorizza spp., Chaetomium spp., Gliocladium spp., Pisolithus spp., Amanita spp., Cantharellus spp., Pythîum spp., and combination thereof.

In an embodiment, the algal extract of the invention may be combined with a second insecticide, fungicide or a herbicide, the choice of which is not particularly iim iting.

In an embodiment, the algal extract of the present invention may be provided as a stable formulation/composition. In this embodiment, the algal extract may be coformuiated with a preservative, the choice of which is not limiting.

Thus, in an embodiment, the present invention provides a composition comprising:

(a) an algal extract having a percentage dry matter content between 18 36%; and (b) a preservative.

Thus, in an embodiment, the present invention provides a composition comprising:

(c) an algal extract having a percentage dry matter content between 24 36%; and (d) a preservative.

Thus, in an embodiment, the present invention provides a composition comprising:

(a) an algal extract having a percentage dry matter content between 24 30%; and (b) a preservative.

In an embodiment, the preservative may be selected from one or more of citric acid monohydrate, sodium methylparaben, potassium sorbate, or sodium metabisulfite.

Thus, in an embodiment, the présent invention provides a composition comprising:

(a) an algal extract having a percentage dry matter content between 18 36%; and (b) one or more of a preservative selected from citric acid monohydrate, sodium methylparaben, potassium sorbate, or sodium metabisulfite.

Thus, in an embodiment, the présent invention provides a composition comprising:

(c) an algal extract having a percentage dry matter content between 24 36%; and (d) one or more of a preservative selected from citric acid monohydrate, sodium methylparaben, potassium sorbate, or sodium metabisulfite.

Thus, in an embodiment, the présent invention provides a composition comprising:

(e) an algal extract having a percentage dry matter content between 24 30%; and (f) one or more of a preservative selected from citric acid monohydrate, sodium methylparaben, potassium sorbate, or sodium metabisulfite.

In an embodiment, the présent invention provides a combination comprising:

(a) an algal extract having a percentage dry matter content between 24 — 30% in an amount greater than 90% by weight of the composition; and (b) a preservative in an amount less than 10% by weight of the composition, wherein the preservative is selected from citric acid monohydrate, sodium methylparaben, potassium sorbate, or sodium metabisulfite.

In an embodiment, the present invention provides a combination comprising:

(a) an algal extract having a percentage dry matter content between 24 -

30% in an amount of about 99.5% by weight of the composition; and (b) citric acid monohydrate in an amount of about 0.5% by weight of the composition.

In an embodiment, the present invention provides a combination comprising:

(a) an algal extract having a percentage dry matter content between 18 36% in an amount of about 99.6% by weight of the composition;

(b) sodium methylparaben in an amount of about 0.1% by weight of the composition;

(c) potassium sorbate in an amount of about 0.1% by weight of the composition; and (d) sodium metabisulfite in an amount of about 0.2% by weight of the composition.

In an embodiment, the present invention provides a combination comprising:

(e) an algal extract having a percentage dry matter content between 24 36% in an amount of about 99.6% by weight of the composition;

(f) sodium methylparaben in an amount of about 0.1% by weight of the composition;

(g) potassium sorbate in an amount of about 0.1% by weight of the composition; and (h) sodium metabisulfite in an amount of about 0.2% by weight of the composition.

In an embodiment, the present invention provides a combination comprising:

(a) an algal extract having a percentage dry matter content between 24 30% in an amount of about 96.6% by weight of the composition;

(b) citric acid in an amount of about 3.0% by weight of the composition;

(c) sodium methylparaben in an amount of about 0.1% by weight of the composition; and (d) sodium metabisulfite in an amount of about 0.2% by weight of the composition.

In an embodiment, the algal extract of the présent invention comprises minerai matter in an amount of about 20% - 45% by weight of the extract.

In an embodiment, the algal extract of the présent invention comprises minerai matter in an amount of about 22% - 41% by weight of the extract.

Ί q In an embodiment, the algal extract of the présent invention comprises minerai matter in an amount of about 31.5% by weight of the extract.

In an embodiment, the algal extract of the présent invention comprises polyphenols in an amount of about 5% - 20% by weight of the extract.

In an embodiment, the algal extract ofthe présent invention comprises polyphenols 15 in an amount of about 8% -19% by weight of the extract.

In an embodiment, the algal extract ofthe présent invention comprises polyphenols in an amount of about 13.5% by weight of the extract.

In an embodiment, the algal extract of the présent invention comprises proteins in an amount of about 1 % - 6% by weight of the extract.

In an embodiment, the algal extract of the présent invention comprises proteins in an amount of about 3.5% by weight of the extract.

In an embodiment, the algal extract of the présent invention comprises mannitol in an amount of about 4% - 23% by weight of the extract.

In an embodiment, the algal extract of the présent invention comprises mannitol in 25 an amount of about 13.5% by weight ofthe extract.

In an embodiment, the algal extract of the present invention comprises fucoidans in an amount of about 1% - 6% by weight of the extract.

In an embodiment, the algal extract of the present invention comprises fucoidans in an amount of about 3.5% by weight of the extract.

In an embodiment, the algal extract of the present invention comprises glucose in an amount of about 3% - 13% by weight of the extract.

In an embodiment, the algal extract of the present invention comprises glucose in an amount of about 8.0% by weight of the extract.

The term substantially free of an alginate means that the algal extract of the present invention comprises less than 0.1% of alginic acid by weight of the extract. Preferably, less than 0.05% of alginic acid is present.

The advantages offered by the present invention will be more apparent from the examples set forth herein below. These examples are provided merely as illustrations of the invention and are not intended to be construed as a limitation thereof.

EXAMPLES

The followîng examples are meant to i11ustrate the present invention. The examples are presented to exemplify the invention and are not to be considered as limiting the scope of the present invention.

Seaweed extract was harvested from North East Atlantic Océan. The extracted seaweed was subjected to crushing and micro-crushing. To the crushed seaweed

0.5% citric acid was added. The mixture was subjected to flocculation under acidic conditions, and fîltered via filter press. The filtrate was concentrated through vacuum évaporation to préparé an extract having 24 - 30% dry matter content.

To the concentrated algal extract was added sodium methyl paraben (0.1%), potassium sorbate (0.1%) and sodium metabisulfite (0.2%). The composition was 25 centrifuged for removal of sédiment and fîltered on diatomaceous earth.

Comparative algal extracts were produced according to Example 1 (5.1%) and Example 2 (13.00%) of US 2017/0251677. A slight variation was seen in the dry matter content as the actual percentage dry matter content was measured to be 4.8% and 9.3% versus 5.1% and 13.0% respectively.

The three algal extracts were then subjected to efficacy tests, and approximate équivalent bio-activity rate in oz/acre for the three algal extracts were noted.

	% Dry Matter	Avg % Dry Matt er	ApproximateEqui valent Bio Activity Rate (oz/ Acre)	Relative efficacy (Calculât ed)
Example 2	8.5 %	10.0 %	9.3 %	32	1
Example 1	4.0 %	5.5 %	4.8 %	8	4
Algal extract ofthe présent invention	24.0 %	30.0 %	27.0 %	1	32

Ii was thus surprisingly found that the algal extract of the présent invention was 32 times more efficacious than example 2, and 8 fîmes more efficacious than example 1 of US 2017/0251677. Thus, the sélection of percentage dry matter content between 24 to 36%, and specificaily about 27.0%, conferred unexpected and surprising efficacy on the algal extract of the présent invention.

Claims (18)

1. A seaweed algal extract comprising dry matter between about 18%-36%.

2. The extract as claimed in claim 1, wherein the seaweed is a coldwater seaweed or brown alga (Phaeophyceae).

5

3. The extract as claimed in claim 1, the said seaweed belongs to family

Fucaceae, preferably genus Ascophyllum.

4. The extract as claimed in claim 3, the said seaweed is Ascophyllum nodosum.

5. A process for préparation of an algal extract having dry matter between about 18%-36%, said process comprising:

1 q a. feeding an initial algal extract comprising dry matter between 3-8% to an evaporator heat exchanger; and

b. passing steam through the heat exchanger to produce the algal extract having dry matter between about 18%-36%,

6. The process as claimed in claim 8, wherein said process further comprises adding one or more preservatives to the algal extract produced in step (b) 15 of the process.

7. The process as claimed in claim S comprising decanting the algal extract produced in step (b) and removing the sédiment produced therefrom to préparé the algal extract.

8. Use of a seaweed extract of Ascophyllum nodosum for biostimulating the 20 plant physiology, wherein said extract is having dry matter between about

18%-36%.

9. A combination comprising an algal extract of Ascophyllum nodosum having dry matter between about 18%-36% and a biostimulating agent or plantprotecting composition, where the plant protecting composition comprises herbicide, fongicide, insecticide or pesticide.

10. A combination comprising an algal extract of Ascophyllum nodosum having dry matter between about 18%-36% and a fertilizer and/or micronutrients.

11. A composition comprising an algal extract of Ascophyllum nodosum having dry matter between about 18%-36% and a super absorbent polymer.

12. A combination comprising an algal extract of Ascophyllum nodosum having dry matter between about 18%-36% and another biostimulant.

5

13. An algal extract of Ascophyllum nodosum having dry matter between about

18-36%.

14. An algal extract of Ascophyllum nodosum having dry matter between about 24%-30%.

15. An algal extract of Ascophyllum nodosum having dry matter between about 25%-29%.

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16. An algal extract of Ascophyllum nodosum having dry matter between about

26%-28%.

17. An algal extract of Ascophyllum nodosum having dry matter content of about 27%.

18. A process comprising:

(a) feeding an initial algal extract comprising dry matter between 3-6% to

15 an evaporator heat exchanger;

(b) passing steam through the heat exchanger to produce the algal extract of the present invention;

(c) adding one or more preservatives to the algal extract produced in step (b); and

2q (d) removing sédiments from the algal extract prepared in step (c) to préparé the algal extract.