OA18640A - Aqueous hypochlorous acid solution. - Google Patents
Aqueous hypochlorous acid solution. Download PDFInfo
- Publication number
- OA18640A OA18640A OA1201800097 OA18640A OA 18640 A OA18640 A OA 18640A OA 1201800097 OA1201800097 OA 1201800097 OA 18640 A OA18640 A OA 18640A
- Authority
- OA
- OAPI
- Prior art keywords
- microbicide
- ppm
- solution
- bacillus
- hypochlorite solution
- Prior art date
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- QWPPOHNGKGFGJK-UHFFFAOYSA-N Hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 title abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- VEXZGXHMUGYJMC-UHFFFAOYSA-N HCl Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000008213 purified water Substances 0.000 claims abstract description 11
- 239000002778 food additive Substances 0.000 claims abstract description 5
- 235000013373 food additive Nutrition 0.000 claims abstract description 5
- WQYVRQLZKVEZGA-UHFFFAOYSA-N Hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 claims description 86
- 239000000243 solution Substances 0.000 claims description 86
- 210000004215 spores Anatomy 0.000 claims description 28
- 241000894006 Bacteria Species 0.000 claims description 18
- 239000000460 chlorine Substances 0.000 claims description 14
- 229910052801 chlorine Inorganic materials 0.000 claims description 14
- ZAMOUSCENKQFHK-UHFFFAOYSA-N chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 14
- 230000003641 microbiacidal Effects 0.000 claims description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 11
- 240000008371 Bacillus subtilis Species 0.000 claims description 7
- 229940075615 Bacillus subtilis Drugs 0.000 claims description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 7
- 241000588724 Escherichia coli Species 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 5
- 241000194108 Bacillus licheniformis Species 0.000 claims description 4
- KEAYESYHFKHZAL-UHFFFAOYSA-N sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims description 4
- 239000011734 sodium Substances 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 claims description 4
- 229940075612 Bacillus cereus Drugs 0.000 claims description 3
- 241000193755 Bacillus cereus Species 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 229940055023 Pseudomonas aeruginosa Drugs 0.000 claims description 2
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims description 2
- 241001354013 Salmonella enterica subsp. enterica serovar Enteritidis Species 0.000 claims description 2
- 239000002855 microbicide agent Substances 0.000 claims 12
- 230000000249 desinfective Effects 0.000 claims 8
- 244000052616 bacterial pathogens Species 0.000 claims 4
- SUKJFIGYRHOWBL-UHFFFAOYSA-N Sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 abstract description 26
- 239000005708 Sodium hypochlorite Substances 0.000 abstract description 26
- 230000000844 anti-bacterial Effects 0.000 abstract description 13
- 239000007858 starting material Substances 0.000 abstract 2
- 238000004166 bioassay Methods 0.000 description 12
- 239000008367 deionised water Substances 0.000 description 12
- 230000000813 microbial Effects 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- AKHNMLFCWUSKQB-UHFFFAOYSA-L Sodium thiosulphate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 239000012488 sample solution Substances 0.000 description 5
- 235000019345 sodium thiosulphate Nutrition 0.000 description 5
- 230000003330 sporicidal Effects 0.000 description 5
- 241000607142 Salmonella Species 0.000 description 4
- 206010039447 Salmonellosis Diseases 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 238000003556 assay method Methods 0.000 description 4
- 230000001580 bacterial Effects 0.000 description 4
- 239000002609 media Substances 0.000 description 4
- NLKNQRATVPKPDG-UHFFFAOYSA-M Potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 239000001965 potato dextrose agar Substances 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 239000001974 tryptic soy broth Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000845 anti-microbial Effects 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- KZBUYRJDOAKODT-UHFFFAOYSA-N chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 201000009910 diseases by infectious agent Diseases 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000001954 sterilising Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 241001648341 Orites Species 0.000 description 1
- 210000003491 Skin Anatomy 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002496 gastric Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002350 laparotomy Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- NPDODHDPVPPRDJ-UHFFFAOYSA-N permanganate Chemical compound [O-][Mn](=O)(=O)=O NPDODHDPVPPRDJ-UHFFFAOYSA-N 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
Abstract
Provided is an aqueous hypochlorous acid solution that has an excellent bactericidal effect and high safety. In the aqueous hypochlorous acid solution according to the present invention that has a pH value within the range of 5.5-6.5, starting materials are dissolved exclusively in purified water specified by japanese pharmacopeia. The starting materials are sodium hypochlorite for food additives and dilute hydrochloric acid specified by japanese pharmacopeia. The pH value of the aqueous hypochlorous acid solution is preferably 6.3.
Description
Technical field
[0001]
The présent invention related to hypochlorite solution having excellent bactericidal effects and safety.
io Background art
[0002]
Conventionally, hypochlorous acid has been utilized for pharmaceuticals, tap water, foods and the like as sodium hypochlorite. Also, hypochlorous acid is commercially available for household products in an aqueous solution form or 15 powder form.
[0003]
The hypochlorite solution is prepared by mixing sodium hypochlorite (NaCIO), dilute hydrochloric acid and water. Both of sodium hypochlorite and tap water are approved substances as food additives, and water is a harmless substance. Therefore, hypochlorite solution is one of highly valued pharmaceutical as the harmless bactericidal agent on a human body. [0004]
Recent years, medical devices directly inserted into human body become popular. It is expected that treatment by using such medical devices gives less burden to a patient compared to thoracotomy or laparotomy, and shorter stay in a hospital. On the other hand, inadéquate sterilization such medical device increases a risk of infection for the patient. Sometimes, the inadequately sterilized medical devices cause nosocomial infection. In order to avoid such infection, hypochlorite acid solution is used for the sterilization of the medical devices. Since hypochlorite solution is strong alkali higher than pH 12, it remained on the sterilized medical devices may irritate human skin of which pH is in a range between pH 4.5 to 6.0.
[0005]
In order to weaken such irritation, sodium hypochlorite solution is usually diluted. However, sodium hypochlorite solution causes the problem that it particularly decomposed at pH not higher than 7. Furthermore, under the acidic condition not higher than pH 5, it rapidly generates chlorine gas. Therefore, devices for inhibiting the chlorine gas disclosed in the patent document 1 or 2 are developed.
Prior Art
Patent Document
[0006]
Patent document 1: JP patent 4740892
Patent Document 2: JP patent 5307351 [0007]
However, the hypochlorite solution, which is prepared by using tap water, slightly includes impurities such as métal and the like. Accordingly, it is desired that the hypochlorite solution has extremely high safety and bactericidal effects, and the solution may be approved as pharmaceuticals. [0008]
The object of the présent invention is to provide the hypochlorite solution having an excellent bactericidal effects and the safety.
Means for solving the problem
[0009]
The hypochlorite solution of the présent invention has the pH range between from 6.0 to 6.5, being prepared by using purified water defined in Japanese Pharmacopoeia.
[0010]
Raw materials for preparing the hypochlorite solution may be sodium hypochlorite as food additives, and dilute hydrochloride defined in Japanese Pharmacopoeia.
[0011] pH of the hypochlorite solution may be 6.0 to 6.5. [0012] pH of the hypochlorite solution may be 6.3. [0013]
The hypochlorite solution may contain 150 to 260 ppm of sodium hypochlorite.
[0014]
The hypochlorite solution may contain 220 ppm of sodium hypochlorite.
Advantageous Effect of the Invention
[0015]
According to the hypochlorite solution of the présent invention, the hypochlorite solution which is useful for the medical supplies and pharmaceuticals has the excellent microbicidal effect and the safety.
Description of the invention
[0016]
Hereinbelow, the hypochlorite solution of the first aspect of the présent invention is explained.
[0017] (Hypochlorite solution and préparation example thereof)
Sodium hypochlorite is weighed so as to be the range from 0.018 to 0.026 w/v %, preferably 0.026 w/v %, and mixed with purified water to préparé sodium hypochlorite solution. The sodium hypochlorite solution is prepared so as to hâve pH 6.0 to 6.5 by using dilute hydrochloric acid (about 9.5 to 10.5 w/v %) and then stirred to mix. The solution may be prepared for containing 150 to 260 ppm of sodium hypochlorite, preferably 220 ppm. For example, the solution may be obtained by using at least 99.9 % of purified water and remained less than 0.1 % of mixture containing about the same amount of sodium hypochlorite solution with effective chlorine concentration 12 % and dilute hydrochloric acid solution with about 10 % concentration thereof, when total amount of the solution is 100 %.
[0018]
Note that in the above example for production, the hypochlorite solution is obtained by mixing the raw materials. However, it may be obtained by using an apparatus for producing the hypochlorite solution, which is commercially available. For example, the patent documents 1 and 2 disclose arts related to such apparatuses.
[0019] (Component analysis of the hypochlorite solution)
Herein below, the table for the component analysis of the hypochlorite solution is shown.
[0020]
[Table 1]
| Items to be checked | Spécifications | Lot Nos. | |||||
| 001 | 002 | 003 | |||||
| Properties | The product is yellow colored liquid without odor, or with faint chlorine odor. | OK | OK | OK | |||
| Validation Test | (1) When 1 mL of sodium hydroxide (2,500 times diluted), and 0.2 mL of potassium iodide test solution are added to 5 mL of the product, the solution turns yellow. When 0.5 mL of starch test solution is further added to the solution, the solution turns deep blue. | OK | OK | OK | |||
| (2) When the 0.1 mL of the permanganate solution (300 times diluted) is added into 5 mL of the product and 1 mL of diluted sulfuric acid (20 times diluted) is added. After that, when 1 mL of the diluted sulfuric acid is further added thereto, red-purple color of the solution is unfaded. | OK | OK | OK | ||||
| (3) The solution prepared by adding 90 mL of the product and 10 mL of sodium hydroxide solution (5 times diluted) has absorption maximum from the | OK | OK | OK | ||||
| wavelength between 290 to 294 | nm. | ||||||
| Purity Test | pH | from 4.5 to 6.5 | No. | 1 | 6.4 | 6.4 | 6.4 |
| 2 | 6.4 | 6.4 | 6.4 | ||||
| 3 | 6.4 | 6.4 | 6.4 | ||||
| Total Residue | not more than 0.25 % | No. | 1 | 0.03 | 0.03 | 0.03 | |
| Quantitative Value | 220 ± 40 ppm | No. | 1 | 256.0 | 259.2 | 251.1 | |
| 2 | 255.5 | 259.5 | 251.4 | ||||
| 3 | 255.6 | 259.2 | 250.9 |
[0021]
Also, the purified water used in the présent invention has following characteristics:
( 1 ) Properties
Colorless and odorless (2) Purity
Not over than 0.50 mg/L under test for total organic carbon (3) Conductivity
When following test is conducted, conductivity at 25 °C is not over than 2.1 μ S/cm.
Adéquate amount of the purified water is poured into a beaker and then stirred. Température of the purified water is adjusted at 25 ± 1 °C, and the conductivity pf the water is measured at regular intervals, vigorously stirring the water. The conductivity of the purified water (25 °C) is set the value when change in conductivity/5 minutes becomes not over than 0.1 μ S/cm.
[0022] (Antimicrobial test for gastrointestinal microbial which occur contamination on a medical endoscope) (1) Assay method
1) Test microbial
Antimicrobial effect of the hypochlorite solution has been confirmed by using the following test microbial.
Escherichia coli (E. coli)
Salmonella enteritidis (Salmonella)
Candida sp. (Candida)
Pseudomonas aeruginosa (P. aeruginosa)
[0023]
2) Préparation of test samples
In order to préparé the test samples, the hypochlorite solution is diluted so as to be the effective chlorine concentration of at 200, 20, 5, 2, 1, 0.5 ppm. Five mL of the test samples are respectively dispensed into 20 mL size test tubes.
Sterilized water without hypochlorite solution is used as a control. [0024]
3) Pre-culture before the assay
For both E. coli and Salmonella, these bacteria are cultivated at 35° C for 20 to 24 hours by standing culture in TSB (Tryptic Soy Broth) to use in the assays.
The assay solutions are adjusted by dilution with sterilized deionized water.
Bacteria numbers in the solutions are 1.2 x 106 / mL for E. coli, and 1.7 x 106 / mL for Salmonella.
For Candida, they are cultured at 25° C for 44 to 48 hours in PDA (Potato Dextrose Agar) medium. Cultured bacteria are suspended in the sterilized deionized water to préparé the bacterial solution. The bacteria number is 2.7 x 5 106 /mL.
For P. aeruginosa, they are cultured by standing culture at 25° C for 44 to 48 hours in TSB. Culture broth is diluted with the sterilized deionized water to préparé the sample solution. The bacteria number is 2.3 x 106 /mL.
[0025]
4) Assay method
In each sample solution with different concentration, 0.2 mL of bacteria solution is inoculated and then mixed. At the time point from the start, 0.5 min., 5 min., and 10 min., 0.2 mL portion is taken out from each sample solution; it is suspended in 1.8 mL of the sterilized deionized water containing 1 mg/mL of sodium thiosulfate. When the solution contains the bacteria, 0.1 mL of the suspension or another sample solution, which is 10-fold diluted solution by using the same sterilized deionized water containing 1 mg/mL of sodium thiosulfate, is streaked on SA medium. When the solution contains east, 0.1 mL of either of the samples prepared as the same as those of bacteria is streaked on PDA medium.
For control samples, the sterilized deionized water is used instead of the sterilized deionized water containing sodium thiosulfate. After the incubation of them, colonies appeared on the plates are counted.
[0026]
5) Evaluation
In order to evaluate the bactericidal effects at each sample with different concentration, appeared colony numbers are measured at each treatment time as viable microbial numbers.
[0027] (2) Assay results
As shown in the following Table 2 to Table 5, ail of the bacterial used in the assays are died by treatment of the hypochlorite solution at the concentration of 5 ppm for 0.5 min.
[0028]
[Table 2]
Bactericidal Effect at each concentration against E. coli (Viable miciObial number)
| ’ —___Treatment time Conc. ' —— | 0.5 min. | 5 min. | 10 min. |
| 200 ppm | 0 | 0 | 0 |
| 20 ppm | 0 | 0 | 0 |
| 5 ppm | 0 | 0 | 0 |
| 2 ppm | 340 | 12 | 0 |
| 1 ppm | 350 | 24 | 0 |
| 0.5 ppm | 310 | 21 | 0 |
Control: 490
[0029]
[Table 3]
Bactericidal Effect at each concentration against Salmonella (Viable microbial number)
| —~----^Treatment time Conc. ~~----—__ | 0.5 min. | 5 min. | 10 min. |
| 200 ppm | 0 | 0 | 0 |
| 20 ppm | 0 | 0 | 0 |
| 5 ppm | 0 | 0 | 0 |
| 2 ppm | 270 | 3 | 0 |
| 1 ppm | 230 | 12 | 0 |
| 0.5 ppm | 270 | 23 | 0 |
Control: 680
[0030]
[Table 4]
Bactericidal Effect at each concentration against Candida (Viable microbial number)
| ' ~---—^Treatment time Conc. | 0.5 min. | 5 min. | 10 min. |
| 200 ppm | 0 | 0 | 0 |
| 20 ppm | 0 | 0 | 0 |
| 5 ppm | 0 | 0 | 0 |
| 2 ppm | 710 | 3 | 0 |
| 1 ppm | 680 | 63 | 0 |
| 0.5 ppm | 880 | 58 | 0 |
Control: l,100
[0031]
[Table 5]
Bactericidal Effect at each concentration against P. aeruginosa (Viable microbial number)
| ——^Treatment time Conc. | 0.5 min. | 5 min. | 10 min. |
| 200 ppm | 0 | 0 | 0 |
| 20 ppm | 0 | 0 | 0 |
| 5 ppm | 0 | 0 | 0 |
| 2 ppm | 660 | 0 | 0 |
| 1 ppm | 550 | 0 | 0 |
| 0.5 ppm | 530 | 0 | 0 |
Control: 920
[0032] (Comparative assay for the bactericidal effects against a variety of spores (formed by bacillus sp.) of the hypochlorite solution described as one embodiment of the présent invention and sodium hypochlorite solution (1) Assay samples
The hypochlorite solution or the sodium hypochlorite solution is prepared as shown in Table 6.
[0033]
[Table 6]
| Effective chlorine conc. (ppm) | Redox potential (mV) | pH | ||
| hypochlorite solution | A | 280 | + 1,140 | 6.3 |
| B | 320 | + 1,140 | 6.7 | |
| C | 57 | + 1,100 | 6.9 | |
| sodium hypochlorite solution | D | 3,300 | +877 | 10.4 |
| E | 1,500 | +940 | 9.9 | |
| F | 350 | +988 | 9.1 |
[0034] (2) Assay method
1) Bacteria for the assay
The comparative assay of the bactericidal effects is conducted by using following bacteria. Here, the bactericidal effects of hypochlorite solution described as one embodiment of the présent invention and sodium hypochlorite solution are compared.
Bacillus subtilis NBRC 13719
Bacillus cereus NBRC 13494
Bacillus licheniformis NBRC 12200
[0035]
2) Préparation of spore solutions
Three bacteria of Bacillus sp. used in the assay are picked up from colonies formed on stores slants, and suspended in the sterilized deionized water. Then the suspension is heated at 80 °C for 15 min. Then, the suspension is streaked on NA (normal agar) plate and incubated at 35 °C for 3 days to form colonies. The bacteria are picked up from the spore rich colonies, and then the suspension is heated at 80 °C for 15 min. The suspension is streaked on NA (normal agar) plate and incubated at 35 °C for 4 to 6 days to form colonies. The bacteria are picked up from the colonies and suspended in the sterilized deionized water. Then, the suspension is heated at 80 °C for 15 min to préparé the spore solution for the assay. The bacteria concentrations in 3 bacteria strains are 1 to 4 x 106 /mL.
[0036]
3) Sporicidal assay method
0.5 mL of the spore solution is inoculated in 4.5 mL of each sample solution, and then mixed. At the time point of 0.5 min., 1 min., 2 min., and 4 min.
from the start, 20 pL portions is taken out from each sample and suspended in 2 mL of the sterilized deionized water containing 1 mg/mL sodium thiosulfate. Then, the suspension is 10-fold diluted by using the same the sterilized deionized water containing sodium thiosulfate, and then streaked on NA medium plates. As Control, the sterilized deionized water is used. The plates were incubated at
35°C for 2 days, and the colonies appeared on the plates were counted.
[0037]
4) Evaluation
The sporicidal effect in each sample with different concentration was evaluated by measuring viable spore number in each treatment time.
[0038] (3) Assay results
1) Sporicidal results in Bacillus subtilis
As shown in the following Table 7, the spores of Bacillus subtilis were died out with 2 minutes treatment, when the sample A or B was used. However, 20 about 1/2 of the spores were survived after 4 minute treatment compared with those of Control, when the sample C was used. About 1/5 to 1/2 of the spores were survived in ail of Control D, Control E, and Control F, when the sodium hypochlorite solution was used. [0039] 25
| [Table 7] Viable spore number produced by Bacillus subtilis sp. after treatment either of the hypochlorite solution or sodium hypochlorite solution ________ | ||||||||
| Effective chlorine conc.(ppm) | pH | Bacteria conc. (cfu/ml) | Treatment time (min.) | |||||
| 0.5 | 1 | 2 | 4 | |||||
| Hypochlorite solution | A | 280 | 6.3 | 1.3 x 105 | 79 | 21 | 0 | 0 |
| B | 320 | 6.7 | 108 | 4 | 0 | 0 | ||
| C | 57 | 6.9 | 100 | 113 | 100 | 57 | ||
| Sodium hypochlorite solution | D | 3,300 | 10.4 | 129 | 108 | 91 | 34 | |
| E | 1,500 | 9.9 | 136 | 104 | 76 | 24 | ||
| F | 350 | 9.1 | 108 | 104 | 84 | 69 | ||
| Control | 0 | 6.4 | 134 | 129 | 121 | 126 | ||
| [0040] |
2) The sporicidal test in Bacillus cereus
As shown in Table 8, the spores of Bacillus subtilis were died out with 1 5 minute treatment or 4 minutes treatment, when the sample A or B was used.
However, some of the spores were survived after 4 minute treatment compared with those of Control, when the sample C was used. About 1/4 to 1/2 of the spores were survived in ail of Control D or Control E, when the sodium hypochlorite solution was used.
[0041]
| [Table 8] Viable spore number of the Bacillus cer< hypochlorite solution or sodium hypoch | ;us after treatment either of the orite solution | |||||||
| Effective chlorine conc.(ppm) | pH | Bacteria conc.(cfu/ml) | Treatment time (min.) | |||||
| 0.5 | 1 | 2 | 4 | |||||
| Hypochlorite solution | A | 280 | 6.3 | 2.0 x 105 | 76 | 0 | 0 | 0 |
| B | 320 | 6.7 | 95 | 32 | 19 | 0 | ||
| C | 57 | 6.9 | 82 | 136 | 107 | 115 | ||
| Sodium hypochlorite solution | D | 3,300 | 10.4 | 119 | 104 | 47 | 0 | |
| E | 1,500 | 9.9 | 127 | 114 | 66 | 0 | ||
| F | 350 | 9.1 | 126 | 111 | 76 | 67 | ||
| Control | 0 | 6.4 | 148 | 186 | 171 | 165 |
[0042]
3) The sporicidal test in Bacillus licheniformis
As shown in Table 9, the spores of Bacillus subtilis were died out with 2 minute treatment, when the sample A or B was used. However, about 1/2 of the spores were survived after 4 minute treatment compared with those of Control, when the sample C was used. About 1/3 to 1/2 of the spores were survived with 4 minute treatment in ail of Control D, Control E, or Control F, when the sodium hypochlorite solution was used. [0043] [Table 9] Viable bacterial number after spores of Bacillus licheniformis was treated with hydrochlorite solution or sodium hydrochlorite solution_________________________
| Effective chloride conc. (ppm) | pH | Initial bacterial number (CFU/mL) | Treatment time (min.) | |||||
| 0.5 | 1 | 2 | 4 | |||||
| Hypochlorite solution | A | 280 | 6.3 | 335 | 37 | 0 | 0 | |
| B | 320 | 6.7 | 266 | 43 | 0 | 0 | ||
| C | 57 | 6.9 | 4.0 x 105 | 355 | 252 | 328 | 237 | |
| Sodium hypochlorite solution | D | 3,300 | 10.6 | 313 | 347 | 293 | 202 | |
| E | 1,500 | 9.9 | 238 | 296 | 184 | 127 | ||
| F | 350 | 9.1 | 322 | 352 | 223 | 233 | ||
| Control | 0 | 6.4 | 349 | 370 | 381 | 393 |
[0044]
The présent invention has the most distinctive characteristic that water added into the hypochlorite solution having mild acidity is only distilled water. As a resuit, the hypochlorite solution having both of excellent microbicidal effects and utility as pharmaceuticals is provided, by using the distilled water as that to be added to the hypochlorite solution. [0045]
It should be noted that the water used for preparing each solution such as sodium hypochlorite solution, distilled hydrochloride solution and the like is only the purified water defined as Japanese Pharmacopoeia.
Industrial applicability
[0046]
As explained above, according to the présent invention, hypochlorite solution having excellent both of bactericidal effects and safety, which is useful as medical supplies or pharmaceuticals are provided. The hypochlorite solution of the présent invention is also utilized as the bactericidal agent in a variety of fields 5 such as a detergent for cooking utensils and the like.
Claims (12)
- Claims1. A microbicide for a Bacillus spore, said microbicide including a hypochlorite aqueous solution comprising chlorine from 280 to 320 ppm as effective chlorine5 concentration, with a pH from 6.3 to 6.7.
- 2. The microbicide for a Bacillus spore according to claim 1, wherein said hypochlorite aqueous solution consists of sodium hydrochlorite as a food additive, purified water as defined in the Japanese Pharmacopoeia, and dilute10 hydrochloric acid solution as defined in the Japanese Pharmacopoeia.
- 3. The microbicide for a Bacillus spore according to claim 1 or claim 2, wherein said Bacillus spore is any spore of genus Bacillus selected from the group consisting of Bacillus subtilis, Bacillus cereus, and Bacillus licheniformis.
- 4. A method for disinfecting a Bacillus spore, wherein a member of interest for disinfection is immersed in a microbicide for aBacillus spore according to any one of claims 1 to 3 for a period from 0.5 minutes to 4 minutes.
- 5. A microbicide for a Bacillus spore, comprising chlorine from 57 to 320 ppm as effective chlorine concentration, with a pH from 6.3 to 6.9.
- 6. A method for disinfecting a Bacillus spore, wherein25 a member of interest for the disinfection is immersed in a microbicide for aBacillus spore according to claim 5 for a period from 0.5 minutes to 4 minutes.
- 7. A microbicide for a microbe, comprising chlorine from 0.5 to 200 ppm of chlorine as effective chlorine concentration, with a pH of 6.4.
- 8. The microbicide according to claim 7, in the form of a hypochlorite aqueousI 18640 ι· ' ! solution consisting of sodium hydrochlorite as a food additive, purified water as defined in theJapanese Pharmacopoeia, and dilute hydrochloric acid solution as defined in the Japanese Pharmacopoeia.5
- 9. The microbicide according to claim 7 or claim 8, wherein said microbe is any bacteria selected from the group consisting of E. coli, Salmonella enteritidis, candida, and Pseudomonas aeruginosa.
- 10. A method for disinfecting a microbe, wherein10 a member of interest for the disinfection is immersed in a microbicide according to any one of daims 7 to 9 for a period from 0.5 minutes to 10 minutes.
- 11. The method for disinfecting a microbe according to claim 10, wherein said member of interest for the disinfection is immersed in said microbicide for
- 15 a period not less than 5 minutes, when the effective chlorine concentration is from0.5 ppm to 5 ppm.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015-182718 | 2015-09-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| OA18640A true OA18640A (en) | 2019-01-31 |
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