OA18639A

OA18639A - Mono- or di-substituted indole derivatives as dengue viral replication inhibitors.

Info

Publication number: OA18639A
Application number: OA1201800091
Authority: OA
Inventors: Tim Hugo Maria Jonckers; Bart Rudolf Romanie Kesteleyn; Jean-François BONFANTI; Pierre Jean-Marie Bernard Raboisson; Arnaud Didier M Marchand; Dorothée Alice Marie-Eve Bardiot
Original assignee: Janssen Pharmaceuticals, Inc.; Katholieke Universiteit Leuven
Priority date: 2015-09-16
Filing date: 2016-09-15
Publication date: 2019-01-31

Abstract

The present invention concerns mono- or disubstituted indole compounds, methods to prevent or treat dengue viral infections by using said compounds and also relates to said compounds for use as a medicine, more preferably for use as a medicine to treat or prevent dengue viral infections. The present invention furthermore relates to pharmaceutical compositions or combination preparations of the compounds, to the compositions or preparations for use as a medicine, more preferably for the prevention or treatment of dengue viral infections. The invention also relates to processes for preparation of the compounds.

Description

The présent invention relates to mono- or di-substituted indole dérivatives, methods to prevent or treat dengue viral infections by using these compounds and also relates to these compounds for use as a medicine, more preferably for use as a medicine to treat or prevent dengue viral infections. The présent invention furthermore relates to pharmaceutical compositions or combination préparations of the compounds, to the compositions or préparations for use as a medicine, more preferably for the prévention or treatment of dengue viral îo infections. The invention also relates to processes for préparation of these compounds.

BACKGROUND OF THE INVENTION

Flaviviruses, which are transmitted by mosquitoes or ticks, cause life- threatening infections in man, such as encephalitis and hémorrhagie fever. Four distinct, but closely related serotypes of the flavivirus dengue are known, so-called DENV1, -2, -3, and -4. Dengue is endemic in most tropical and subtropical régions around the world, predominantly in urban and semi-urban areas. According to the World Health Organization (WHO), 2.5 billion people of which 1 billion children are at risk of DENV infection (WHO, 2002). An estimated 50 to 100 million cases of dengue fever [DF], half a million cases of severe dengue disease (i.e. dengue hémorrhagie fever [DHF] and dengue shock syndrome [DSS]), and more than 20,000 deaths occur worldwide each year. DHF has become a leading cause of hospitalization and death amongst children in endemic régions. Altogether, dengue represents the most common cause of arboviral disease. Because of recent large outbreaks in countries situated in Latin America, South-East Asia and the Western Pacific (including Brazil, Puerto Rico, Venezuela, Cambodia, Indonesia, Vietnam, Thailand), numbers of dengue cases hâve risen dramatically over the past years. Not only is the number of dengue cases increasing as the disease is spreading to new areas, but the outbreaks tend to be more severe.

To prevent and/or control the disease associated with dengue viral infection, the only available methods at présent are mosquito éradication strategies to control the vector. Although progress is being made in the development of vaccines against dengue, many difficulties are encountered. These include the

-2existence of a phenomenon referred to as antibody-dependent enhancement (ADE).

Recovery from an infection by one serotype provides lifelong immunity against that serotype but confers only partial and transient protection against a subséquent infection by one of the other three serotypes. Following infection with another serotype, pre-existing heterologous antibodies form complexes with the newly infecting dengue virus serotype but do not neutralize the pathogen. Instead, virus entry into cells is believed to be facilitated, resulting in uncontrolled virus réplication and higher peak viral titers. In both primary and ίο secondary infections, higher viral titers are associated with more severe dengue disease. Since maternai antibodies can easily pass on to infants by breast feeding, this might be one of the reasons that children are more affected by severe dengue disease than adults.

In locations with two or more serotypes circulating simultaneously, also referred to as hyper endemic régions, the risk of serious dengue disease is significantly higher due to an increased risk of experiencing a secondary, more severe infection. Moreover, in a situation of hyper-endemicity, the probability of the emergence of more virulent strains is increased, which in turn augments the probability of dengue hémorrhagie fever (DHF) or dengue shock syndrome.

The mosquitoes that carry dengue, including Aedes aegypti and Aedes albopictus (tiger mosquito), are moving north on the globe. According to the United States (US) Centers for Disease Control and Prévention (CDC), both mosquitoes are currently omniprésent in Southern Texas. The spread north of dengue-carrying mosquitoes is not confined to the US, but has also been observed in Europe.

Recently (December 2015), the dengue vaccine produced by Sanofi Pasteur was first approved in Mexico. The vaccine has also been approved in Brazil, The Philippines and El Salvador. Regulatory review processes are continuing in other countries where dengue is a public health priority. Nevertheless, the vaccine leaves considérable room for improvement due to limited efficacy, especially against DENV-1 and -2, low efficacy in flavivirus-naïve subjects and the lengthy dosing schedule.

Despite these shortcomings, the vaccine is a game changer in endemic settings as it will offer protection to a large part of the population, but likely not

-3to very young infants, who bear the largest burden of dengue. In addition, the dosing schedule and very limited efficacy in flavivirus-naïve subjects make it unsuitable and likely not worthwhile/cost-effective for travelers from nonendemic areas to dengue-endemic areas. The above mentioned shortcomings 5 of the dengue vaccines are the reason why there is a need for a pre-exposure prophylactic dengue antiviral.

Furthermore, today, spécifie antiviral drugs for the treatment or prévention of dengue fever virus infection are not available. Clearly, there is still a great ίο unmet medical need for therapeutics for the prévention or treatment of viral infections in animais, more in particular in humans and especially for viral infections caused by Flaviviruses, more in particular Dengue virus. Compounds with good anti-viral potency, no or low levels of side-effects, a broad spectrum activity against multiple Dengue virus serotypes, a low toxicity and/or good 15 pharmacokinetic or -dynamic properties are highly needed.

The présent invention now provides compounds, mono- ordi-substituted indole dérivatives, which show high potent activity against ail four (4) serotypes of the Dengue virus. Also the compounds according to the invention possess a good 20 pharmacokinetic profile and surprisingly these spécifie compounds show an improved chiral stability.

SUMMARY OF THE INVENTION

The présent invention is based on the unexpected finding that at least one of 25 the above-mentioned problems can be solved by the current compounds of the invention.

The présent invention provides compounds which hâve been shown to possess potent antiviral activity against ail four (4) serotypes currently known. The présent invention furthermore demonstrates that these compounds efficiently 30 inhibit prolifération of Dengue virus (DENV). Therefore, these compounds constitute a useful class of potent compounds that can be used in the treatment and/or prévention of viral infections in animais, mammals and humans, more specifically for the treatment and/or prévention of infections with Dengue viruses.

The présent invention furthermore relates to the use of such compounds as medicines and to their use for the manufacture of médicaments for treating and/or preventing viral infections, in particular with viruses belonging to the

-4family of the Dengue viruses in animais or mammals, more in particular in humans. The invention also relates to methods for the préparation of ail such compounds and to pharmaceutical compositions comprising them in an effective amount.

The présent invention also relates to a method of treatment or prévention of dengue viral infections in humans by the administration an effective amount of one or more such compounds, or a pharmaceutically acceptable sait thereof optionally in combination with one or more other medicines, like another antiviral agent, to a patient in need thereof.

One aspect of the invention is the provision of compounds of formula (I) a stereo-isomeric form, a pharmaceutically acceptable sait, solvaté or polymorph thereof comprising a mono- ordi-substituted indole group; said compound is selected from the group wherein:

Ri is H, R₂ is F, Cl or OCH₃ and R₃ is H;

Ri is H, R₂ is F or Cl and R₃ is CH₃;

Ri is CH₃, R₂ is OCH₃ and R₃ is H;

Ri is F, R₂ is F and R₃ is H;

Ri is CH₃, R₂ is F and R₃ is H;

Ri is CF₃ or OCF₃ and R₂ is H and R₃ is H;

Ri is OCF₃, R₂ is OCH₃ and R₃ is H or

R! is OCF₃ , R₂ is H and R₃ is CH₃.

In particular the compounds of the invention or their stereo-isomeric form, a pharmaceutically acceptable sait, solvaté or polymorph thereof are selected from the group:

Part of the current invention is also a pharmaceutical composition comprising a compound of formula (I) or a stéréo- isomeric form , a pharmaceutically acceptable sait, solvaté or polymorph thereof together with one or more pharmaceutically acceptable excipients, diluents or carriers.

-6Pharmaceutically acceptable salts of the compounds of formula (I) include the acid addition and base salts thereof. Suitable acid addition salts are formed from acids which form non-toxic salts. Suitable base salts are formed from bases which form non-toxic salts.

The compounds of the invention may also exist in un-solvated and solvated forms. The term “solvaté” is used herein to describe a molecular complex comprising the compound ofthe invention and one or more pharmaceutically acceptable solvent molécules, for example, éthanol.

ίο The term “polymorph” refers to the ability of the compound of the invention to exist in more than one form or crystal structure.

The compounds ofthe présent invention may be administered as crystalline or amorphous products. They may be obtained for example as solid plugs, powders, or films by methods such as précipitation, crystallization, freeze drying, spray drying, or evaporative drying. They may be administered alone or in combination with one or more other compounds of the invention or in combination with one or more other drugs. Generally, they will be administered as a formulation in association with one or more pharmaceutically acceptable excipients. The term “excipient” is used herein to describe any ingrédient other than the compound(s) of the invention. The choice of excipient dépends largely on factors such as the particular mode of administration, the effect of the excipient on solubility and stability, and the nature ofthe dosage form.

The compounds of the présent invention or any subgroup thereof may be 25 formulated into various pharmaceutical forms for administration purposes. As appropriate compositions there may be cited ail compositions usually employed for systemically administering drugs. To préparé the pharmaceutical compositions ofthis invention, an effective amountofthe particularcompound, optionally in addition sait form, as the active ingrédient is combined in intimate 30 admixture with a pharmaceutically acceptable carrier, which carrier may take a wide variety of forms depending on the form of préparation desired for administration. These pharmaceutical compositions are desirably in unitary dosage form suitable, for example, for oral or rectal administration. For example, in preparing the compositions in oral dosage form, any ofthe usual 35 pharmaceutical media may be employed such as, for example, water, glycols, oils, alcohols and the like in the case of oral liquid préparations such as suspensions, syrups, élixirs, émulsions, and solutions; or solid carriers such as starches, sugars, kaolin, diluents, lubricants, binders, disintegrating agents and

-7the like in the case of powders, pills, capsules, and tablets. Because of their ease in administration, tablets and capsules represent the most advantageous oral dosage unit forms, in which case solid pharmaceutical carriers are obviously employed. Also included are solid form préparations that can be converted, shortly before use, to liquid forms.

It is especially advantageous to formulate the aforementioned pharmaceutical compositions in unit dosage form for ease of administration and uniformity of dosage. Unit dosage form as used herein refers to physically discrète units suitable as unitary dosages, each unit containing a predetermined quantity of active ingrédient calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. Examples of such unit dosage forms are tablets (including scored or coated tablets), capsules, pills, powder packets, wafers, suppositories, injectable solutions or suspensions and the like, and segregated multiples thereof.

Those of skill in the treatment of infectious diseases will be able to détermine the effective amount from the test results presented hereinafter. In general it is contemplated that an effective daily amount would be from 0.01 mg/kg to 50 mg/kg body weight, more preferably from 0.1 mg/kg to 10 mg/kg body weight. It may be appropriate to administer the required dose as two, three, four or more sub-doses at appropriate intervals throughout the day. Said subdoses may be formulated as unit dosage forms, for example, containing 1 to 1000 mg, and in particular 5 to 200 mg of active ingrédient per unit dosage form.

The exact dosage and frequency of administration dépends on the particular compound of formula (I) used, the particular condition being treated, the severity of the condition being treated, the âge, weight and general physical condition of the particular patient as well as other médication the individual may be taking, as is well known to those skilled in the art. Furthermore, it is évident that the effective amount may be lowered or increased depending on the response of the treated subject and/or depending on the évaluation of the physician prescribing the compounds of the instant invention. The effective amount ranges mentioned above are therefore only guidelines and are not intended to limit the scope or use of the invention to any extent.

-8The présent disclosure is also intended to include any isotopes of atoms présent in the compounds of the invention. For example, isotopes of hydrogen include tritium and deuterium and isotopes of carbon include C-13 and C-14.

The présent compounds used in the current invention may also exist in their stereo-chemically isomeric form, defining ail possible compounds made up of the same atoms bonded by the same sequence of bonds but having different three-dimensional structures, which are not interchangeable. Unless otherwise mentioned or indicated, the Chemical désignation of compounds encompasses the mixture of ail possible stereo-chemically isomeric forms, which said compounds might possess.

Said mixture may contain ail dia-stereomers and/or enantiomers of the basic molecular structure of said compound. Ail stereo-chemically isomeric forms of the compounds used in the présent invention either in pure form or in admixture 15 with each other are intended to be embraced within the scope of the présent invention including any racemic mixtures or racemates.

Pure stereoisomeric forms of the compounds and intermediates as mentioned herein are defined as isomers substantially free of other enantiomeric or diastereomeric forms of the same basic molecular structure of said compounds or intermediates. In particular, the terrn 'stereoisomerically pure' concerns compounds or intermediates having a stereoisomeric excess of at least 80% (i. e. minimum 90% of one isomer and maximum 10% of the other possible isomers) up to a stereoisomeric excess of 100% (i.e. 100% of one isomer and none of the other), more in particular, compounds or intermediates having a stereoisomeric excess of 90% up to 100%, even more in particular having a stereoisomeric excess of 94% up to 100% and most in particular having a stereoisomeric excess of 97% up to 100%. The terms 'enantiomerically pure' and 'diastereomerically pure' should be understood in a 30 similar way, but then having regard to the enantiomeric excess, respectively the diastereomeric excess of the mixture in question.

Pure stereoisomeric forms of compounds and intermediates used in this invention may be obtained by the application of art-known procedures. For 35 instance, enantiomers may be separated from each other by the sélective crystallization of their diastereomeric salts with optically active acids or bases. Examples thereof are tartaric acid, dibenzoyltartaric acid, ditoluoyltartaric acid and camphosulfonic acid. Alternatively, enantiomers may be separated by

-9chromatographic techniques using chiral stationary phases. Said pure stereochemically isomeric forms may also be derived from the corresponding pure stereochemically isomeric forms of the appropriate starting materials, provided that the reaction occurs stereospecifically. Preferably, if a spécifie 5 stereoisomer is desired, said compound will be synthesized by stereospecific methods of préparation. These methods will advantageously employ enantiomerically pure starting materials.

General svnthetic aoproaches îo The synthesis of compounds of general formula I can be performed as outlined in Scheme 1. A 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)acetic acid dérivative of general formula II, containing an O-protecting group PG of the hydroxyl function (PG may be for example an O-benzyl protecting group), can be converted to the corresponding acid chloride dérivative of general formula III with a chlorination reagent like for example oxalyl chloride or thionyl chloride. The Friedel-Crafts reaction of the acid chloride of general formula III with a substituted indole of general formula IV can be performed using a Lewis acid reagent like for example Et₂AICI in a suitable solvent like for example CH₂CI₂, and under suitable reaction conditions that typically involve cooling, to provide the 3-acylated indole of general formula V. Removal of the protecting group PG from the compounds of general formula V can be performed by for example reductive hydrogenolysis (PG = benzyl) in a suitable solvent like for example EtOAc, to provide the compounds of general formula VI. The introduction of an aniline moiety in alpha position to the carbonyl moiety of the compounds of general formula VI can be accomplished by a reaction sequence that involves for example bromination of VI with a reagent like for example phenyltrimethylammonium tribromide in a suitable solvent like for example THF, to provide the compounds of general formula VII, and subséquent reaction of the compounds of general formula VII with 3-methoxy-5-(methylsulfonyl)aniline (VIII) in a suitable solvent like for example CHaCN, and optionally using a base like for example TEA or DIPEA, to provide the compounds of general formula I as racemic mixtures. The chiral séparation of the compounds of general formula I can be performed by for example chiral chromatography to provide the Enantiomers A and B of general formula I.

Chiral séparation

Enantiomers l(A) and l(B)

Scheme 1

Alternatively, the conversion of the intermediates of general formula V to the Compounds of general formula I can also be accomplished by the reaction sequence outlined in Scheme 2: bromination at the alpha position of the carbonyl function of the intermediates of general formula V with a suitable bromination reagent such as for example phenyltrimethylammonium tribromide in a suitable solvent like for example THF, provides the compounds of general formula IX. Subséquent reaction of the compounds of general formula IX with 3-methoxy-5-(methylsulfonyl)aniline (VIII) in a suitable solvent like for example CH3CN, and optionally using a base like for example TEA or DIPEA, provides the compounds of general formula X. After removal of the O-protecting group (PG) from the compounds of general formula X by for example reductive hydrogenolysis (PG = benzyl) in suitable solvent like for example EtOAc or MeOH, the compounds of general formula I are generated as racemic mixtures. The chiral séparation of the compounds of general formula I can be performed by for example chiral chromatography to provide the Enantiomers A and B of general formula I.

-11ci

Chiral séparation

Enantiomers l(A) and l(B)

Scheme 2

In a third approach, the synthesis of the Compounds of general formula I can be accomplished by the reaction sequence outlined in Scheme 3. This approach uses a one-pot three component reaction as the key reaction step to compose the central scaffold of the compounds of general formula I: condensation of 2-(2-(benzyloxy)ethoxy)-4-chlorobenzaldehyde (XI) with

3-methoxy-5-(methylsulfonyl)aniline (VIII) in a suitable solvent such as for example EtOH provides the intermediate imine XII, which upon addition of the substituted N-Boc-protected indole-carboxaldehyde of general formula XIII, and in the presence of an Umpolung catalyst such as for example 3-benzyl-5(2-hydroxyethyl)-4-methylthiazol-3-ium, provides the compounds of general formula X. After removal of the O-protecting group (PG) from the compounds of general formula X by for example reductive hydrogenolysis (PG = benzyl) in suitable solvent like for example EtOAc or MeOH, the compounds of general formula I are generated as racemic mixtures. The chiral séparation of the compounds of general formula I can be performed by for example chiral chromatography to provide the Enantiomers A and B of general formula I.

Chiral séparation

Enantiomers l(A) and l(B)

Scheme 3

Exemples

LC/MS methods

The High Performance Liquid Chromatography (HPLC) measurement was performed using a LC pump, a diode-array (DAD) or a UV detector and a column as specified in the respective methods. If necessary, additional detectors were included (see table of methods below).

Flow from the column was brought to the Mass Spectrometer (MS) which was configured with an atmospheric pressure ion source. It is within the knowledge of the skilled person to set the tune parameters (e.g. scanning range, dwell time...) in order to obtain ions allowing the identification of the compound’s nominal monoisotopic molecular weight (MW). Data acquisition was performed with appropriate software.

Compounds are described by their experimental rétention times (R_t) and ions. If not specified differently in the table of data, the reported molecular ion corresponds to the [M+H]⁺ (protonated molécule) and/or [M-H]' (deprotonated molécule). In case the compound was not directly ionizable the type of adduct is specified (i.e. [Μ+ΝΗ4Γ, [M+HCOO]', etc...). For molécules with multiple

-13isotopic patterns (Br, Cl), the reported value is the one obtained for the lowest isotope mass. AH results were obtained with experimental uncertainties that are commonly associated with the method used.

Hereinafter, “SQD” means Single Quadrupole Detector, “MSD” Mass Sélective

Detector, “RT' room température, “BEH” bridged ethylsiloxane/silica hybrid, DAD” Diode Array Detector, ”HSS” High Strength silica.

LC/MS Method codes (Flow expressed in mL/min; column température (T) in °C; Run time in minutes)

Method code	Instrument	Column	Mobile phase	Gradient	Flow Col T	Run time (min)
LC-A	Waters: Acquity® UPLC DAD-SQD	Waters: BEH C18 (1.7pm, 2.1x50mm)	A: 10mM CH3COONH4 in 95% H₂O + 5% CH₃CN B: CH₃CN	From 95% A to 5% A in 1.3 min, held for 0.7 min.	0.8 mL/min 55°C	2
LC-B	Waters: Acquity® UPLC®DAD-SQD	Waters: HSS T3 (1.8pm, 2.1x100mm)	A: 10mM CH₃COONH₄in 95% H₂O + 5% CH₃CN B: CH₃CN	From 100% A to 5% Ain 2.10 min, to 0% A in 0.90 min, to 5% A in 0.5 min	0.7 mL/min 55°C	3.5
LC-C	Waters: Acquity® UPLC®- DADQuattro Micro™	Waters: BEH C18(1.7pm, 2.1x100mm)	A: 95% CH3COONH4 7m M / 5% CH3CN, B: CH₃CN	84.2% A for 0.49 min, to 10.5% A in 2.18 min, held for 1.94 min, back to 84.2% A in 0.73 min, held for 0.73 min.	0.343 mL/min 40°C	6.2
LC-D	Waters: Acquity® UPLC®- DADAcquity®TQ detector	Waters: BEHC18 (1.7pm, 2.1x50mm)	A: 10 mM CH3COONH4 , pH 10 B: CH₃CN	80% A to 40% A in 3.4 min, to 10% A in 0.6 min, held for 1 min.	0.5 mL/min 40°C	5
LC-E	Waters: Acquity’® UPLC®DADAcquity®TQ detector	Waters: BEH C18 (1.7pm, 2.1x50mm)	A: 10 mM CH3COONH4 , pH 10 B: CH₃CN	50% A to 10% A in 3.5 min, held for 1.5 min.	0.5 mL/min 40°C	5

-14SFC/MS methods

The SFC measurement was performed using an Analytical Supercritical fluid chromatography (SFC) System composed by a binary pump for delivering carbon dioxide (CO2) and modifier, an autosampler, a column oven, a diode array detector equipped with a high-pressure flow cell standing up to 400 bars. If configured with a Mass Spectrometer (MS) the flow from the column was brought to the (MS). It is within the knowledge of the skilled person to set the tune parameters (e.g. scanning range, dwell time...) in order to obtain ions îo allowing the identification of the compound's nominal monoisotopic molecular weight (MW). Data acquisition was performed with appropriate software. Analytical SFC/MS Methods (Flow expressed in mL/min; column température (T) in °C; Run time in minutes, Backpressure (BPR) in bars.

Method code	column	mobile phase	gradient	Flow Col T	Run time BPR
SFC-A	Daicel Chiralpak® IC column (5 pm, 150 x 4.6 mm)	A:CO₂B: MeOH (+0.3% iPrNH₂)	40% B hold 7 min,	3 35	7 100
SFC-B	Daicel Chiralcel® OD-3 column (3 pm, 100 x 4.6 mm)	A:CO₂B: EtOH (+0.3% iPrNH₂)	30% B hold 7 min,	3.5 35	6 103
SFC-C	Daicel Chiralpak® AS3 column (3 pm, 150 x 4.6 mm)	A: CO₂B: EtOH +0.2% iPrNH₂ +3% H2O	10%-50% B in 6 min, hold 3.5 min	2.5 40	9.5 110
SFC-D	Daicel Chiralpak® AS-H column (5 pm, 250 x 4.6 mm)	A: CO₂B: EtOH +0.2% iPrNH₂	35% B hold 4 min, to 50% in 1 min, hold 2 min	5 40	7 110
SFC-E	Daicel Chiralpak® IC column (5 pm, 150 x 4.6 mm)	A:CO₂ B: MeOH	25% B hold 7 min,	3 35	7 100

Method code	column	mobile phase	gradient	Flow Col T	Run time
BPR
SFC-F	Daicel Chiralpak® IA column (5 pm, 150 x 4.6 mm)	A:CO₂ B: iPrOH +0.3% iPrNH₂	30% B hold 7 min,	3	7
35	100

Meltinq Points

Values are either peak values or melt ranges, and are obtained with experimental uncertainties that are commonly associated with this analytical 5 method.

DSC823e (indicated as DSC)

For a number of compounds, melting points were determined with a DSC823e (Mettler-Toledo). Melting points were measured with a température gradient of 10°C/minute. Maximum température was 300°C.

Optical Rotations:

Optical rotations were measured on a Perkin-Elmer 341 polarimeter with a sodium lamp and reported as follows: [α]° (λ, c g/100ml, solvent, T°C).

[a]/ = (100a) I (Ixc) : where / is the path length in dm and c is the concentration in g/100 ml for a sample at a température T (°C) and a wavelength λ (in nm). If the wavelength of light used is 589 nm (the sodium D line), then the Symbol D might be used instead. The sign of the rotation (+ or -) should always be given. When using this équation the concentration and solvent are always provided in parenthèses after the rotation. The rotation is reported using degrees and no units of concentration are given (it is assumed to be g/100 ml).

Example 1 : synthesis of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-fluoro-1/-/indol-3-yl)-2-((3-methoxy-5-(methylsulfonyl)phenyl)amino)ethanone (Compound

1) and chiral séparation into Enantiomers 1A and 1B.

2) EtOH, rt 1h

1) (COCl)₂THF, rt 30 min

BBr₃, CH₂CI₂

-30 to -20°C, 1h

« ^OBn Br Cs₂CO₃

DMF, rt overnight

0.5N NaOH

rt overnight

SOCl₂

EtOH, THF rt 3h

Synthesis of intermediate 1a:

A solution of 2-(4-chloro-2-methoxyphenyl)acetic acid [CAS 170737-95-8] (20 g, 5 101 mmol) in dry THF (300 mL) was cooled at 0°C. Oxalyl chloride (18 mL,

202 mmol) and two drops of DMF were added. The reaction mixture was stirred at room température for 30 min. The solvent was evaporated under reduced pressure. The residue was dissolved in éthanol (300 mL) and the reaction mixture was stirred at room température for 1 h. The reaction mixture was concentrated under reduced pressure to give ethyl 2-(4-chloro-2methoxyphenyl)acetate 1a (23 g), which was used in the next step without further purification.

-17Synthesis of intermediate 1b:

To a solution of ethyl 2-(4-chloro-2-methoxyphenyl)acetate 1a (10 g, 44 mmol) in CH2CI2 (350 mL), cooled at -30 °C, was added dropwise a 1M BBr₃ solution in CH2CI2 (87.5 mL, 87.5 mmol) while maintaining the température below -20 °C. The reaction mixture was stirred at -30 °C for 1 h before quenching with methanol. The pH was adjusted to 8 by addition of an aqueous saturated solution of NaHCO₃. The phases were separated. The aqueous phase was extracted with CH2CI2. The organic phases were combined, dried over MgSO4, filtered and concentrated under reduced pressure to afford ethyl 10 2-(4-chloro-2-hydroxyphenyl)acetate 1b (9.5 g), which was used in the next _c step without further purification.

Synthesis of intermediate 1c:

To a mixture of ethyl 2-(4-chloro-2-hydroxyphenyl)acetate 1b [CAS 126182615 30-5] (2.82 g, 13.1 mmol) and césium carbonate (8.56 g, 26.3 mmol) in DMF (50 mL) was added benzyl 2-bromoethyl ether [CAS 1462-37-9] (2.29 g, 14.5 mmol). The reaction mixture was stirred at room température for 24 h. H₂O was added and the reaction mixture was extracted with EtOAc. The organic phase was dried over Na₂SO4, filtered and concentrated under reduced pressure. The residue was purified by flash chromatography on silica gel using a gradient of EtOAc (2% to 20%) in heptane to give ethyl 2-(2-(2(benzyloxy)ethoxy)-4-chlorophenyl)acetate 1c (4.17 g).

Synthesis of intermediate 1d:

To a solution of ethyl 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)acetate 1c (4.17 g, 12.0 mmol) in a mixture of EtOH (80 mL) and THF (40 mL) was added 0.5N NaOH (72 mL, 36.0 mmol). The reaction mixture was stirred at room température for 3 h. The reaction mixture was partially concentrated under reduced pressure to remove the organic solvents. The residue was acidified to pH 2-3 with 1N HCl and the mixture was extracted with EtOAc. The organic phase was dried over MgSO4, filtered and concentrated under reduced pressure to give 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)acetic acid 1d (3.83 g).

Synthesis of intermediate 1e:

A solution of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)acetic acid 1d (7.12 g, 22.2 mmol) in thionyl chloride (50 mL, 689 mmol) was stirred at room température for 18 h. The reaction mixture was concentrated under reduced

-18pressure and co-evaporated with toluene to give 2-(2-(2-(benzyloxy)ethoxy)-4chlorophenyl)acetyl chloride 1e (7.53 g) which was used in the next step without further purification.

Synthesis of intermediate 1f:

Diethylaluminum chloride 1M in hexane (22.2 mL, 22.2 mmol) was added dropwise, at 0°C, to a solution of 6-fluoro-1/-/-indole [CAS 399-51-9] (2 g, 14.8 mmol) in CH2CI2 (100 mL). After stirring for 15 min at 0°C, a solution of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)acetyl chloride 1e (7.53 g,

22.2 mmol) in dichloromethane (75 mL) was added dropwise over 1 h, while keeping the internai température of the reaction mixture below 4°C. The reaction mixture was stirred at 0°C for 2 h. A solution of potassium sodium tartrate tetrahydrate (Rochelle sait) [CAS 6100-16-9] (8.35 g, 29.6 mmol) in water (9 mL) was added dropwise. The reaction mixture was stirred at 0°C for

20 min. THF (200 mL) and Na2SO₄ (35 g) were added and the mixture was stirred for 3 h at room température. The reaction mixture was filtered over dicalite® and the filter cake was washed several times with THF. The combined filtrâtes were evaporated under reduced pressure and co-evaporated with CH3CN. The solid residue was stirred up in CH3CN (20 mL) at 40°C. The precipitate was filtered off, the solids were washed with CH3CN (3x 3 mL) and dried under vacuum at 45°C to give crude compound 1f (2.48 g). Concentration of the filtrate provided a second crop of 1f (0.9 g). The combined fractions (3.4 g) were re-crystallized from EtOAc. The precipitate was filtered off, washed with EtOAc (3x) and dried under vacuum at 45°C to provide 2-(2-(225 (benzyloxy)ethoxy)-4-chlorophenyl)-1-(6-fluoro-1H-indol-3-yl)ethanone 1f (2.21g).

Synthesis of intermediate 1g:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(6-fluoro-1H-indol30 3-yl)ethanone 1f (2.21 g, 5.05 mmol) and 10% palladium on carbon (1 g) in EtOAc (75 mL) and THF (40 mL) was stirred at room température for 10 min under H2 atmosphère. The reaction mixture was filtered over dicalite® and the filtrate was concentrated under reduced pressure. The residue was crystallized from a mixture of THF (10 mL) and diisopropyl ether (20 mL). The precipitate was filtered off, washed with diisopropyl ether (3x) and dried under vacuum at 50°C to provide 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-fluoro-1H-indol3-yl)ethanone 1g (1.52 g).

-19Synthesis of intermediate 1h:

Phenyltrimethylammonium tribromide [CAS 4207-56-1] (1.73 g, 4.59 mmol) was added in portions to a cooled (0°C) solution of 2-(4-chloro-2-(2hydroxyethoxy)phenyl)-1-(6-fluoro-1/-/-indol-3-yl)ethanone 1g (1.52 g,

4.37 mmol) in THF (60 mL). The mixture was stirred at 0°C for 45 min and at room température for 90 min. The precipitate was filtered off and washed with THF. The combined filtrâtes were concentrated under reduced pressure to give 2-bromo-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-fluoro-1/-/-indol-3yl)ethanone 1 h (1.87 g) which was used without further purification in the next ίο step.

Synthesis of Compound 1 and chiral séparation into Enantiomers 1A and 1B:

A mixture of 2-bromo-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-fluoro-1H15 indol-3-yl)ethanone 1h (1.87 g, 4.37 mmol), 3-methoxy-5(methylsulfonyl)aniline [CAS 62606-02-4] (1.76 g, 8.74 mmol) and diisopropylethylamine (1.51 mL, 8.74 mmol) in CH3CN (125 mL) was stirred at 60°C for 20 h. The reaction mixture was diluted with water (500 mL) and extracted with Et₂O (2x). The combined organic fractions were washed with brine, dried over MgSÛ4 and evaporated under reduced pressure. The residue was purified by flash chromatography (Stationary phase: Grâce Reveleris® silica 80 g, Mobile phase: heptane/EtOAc/EtOH gradient 100/0/0 to 40/45/15). The desired fractions were combined, evaporated under reduced pressure, and co-evaporated with CH3CN. The residue was purified via Reverse Phase HPLC (Stationary phase: Kromasil® C18 100A 5 pm (Eka Nobel), Mobile phase: Gradient from 50% ammoniumbicarbonate in water (0.25%), 50% acetonitrile to 0% ammoniunbicarbonate in water (0.25%), 100% acetonitrile). The product fractions were combined and evaporated under reduced pressure to provide Compound 1 (1400 mg) as a racemic mixture.

The chiral séparation of the enantiomers of Compound 1 (1400 mg) was performed via Normal Phase Chiral séparation (Stationary phase: (S,S) Whelk01,5 pm with recycling peak shaving technique, Mobile phase: 100% éthanol). The product fractions were combined and evaporated under reduced pressure to provide Enantiomer 1A (588 mg) as the first eluted product and Enantiomer

1B (466 mg) as the second eluted product. Enantiomer 1A was purified by flash chromatography (Stationary phase: Grâce Reveleris® silica 12 g, Mobile phase: heptane/EtOAc/EtOH gradient 100/0/0 to 40/45/15). The desired fractions were combined and evaporated under reduced pressure. The residue

-20was stirred up in H2O (7.5 mL) + MeOH (2.5 mL). The solids were filtered off, washed with a mixture of H2O/MeOH 3/1 (3x), and dried at under vacuum at 45°C to provide Enantiomer 1A (0.425 g). Enantiomer 1B was purified by flash chromatography (Stationary phase: Grâce Reveleris® silica 12 g, Mobile phase:

heptane/EtOAc/EtOH gradient 100/0/0 to 40/45/15). The desired fractions were combined and evaporated under reduced pressure. The residue was stirred up in H₂O (7.5 mL) + MeOH (2.5 mL). The solids were filtered off, washed with a mixture of H₂O/MeOH 3/1 (4x), and dried under vacuum at 45°C to provide Enantiomer 1B (0.375 g).

Compound 1:

¹H NMR (300 MHz, DMSO-d₆) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.85 - 4.09 (m, 2 H) 4.20 (t, J=4.3 Hz, 2 H) 5.30 (t, J=4.9 Hz, 1 H) 6.38 (d, J=7.5 Hz, 1 H) 6.58 (s, 1 H) 6.66 (s, 1 H) 6.91 - 7.00 (m, 2 H) 7.01 - 7.15 (m, 3 H) 7.25 (dd, J=9.4, 2.3 Hz, 1 H) 7.37 (d, J=8.3 Hz, 1 H) 8.16 (dd, J=8.7, 5.7 Hz, 1 H) 8.68 (s, 1 H) 12.17 (br. s., 1 H)

LC/MS (method LC-D): R_t 3.70 min, MH⁺547

Enantiomer 1A:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 3.08 (s, 3 H) 3.72 (s, 3 H) 3.88 - 4.05 (m, 2 H) 4.20 (t, J=4.6 Hz, 2 H) 5.26 (t, J=5.5 Hz, 1 H) 6.37 (d, J=7.8 Hz, 1 H) 6.58 (t, J=1.9 Hz, 1 H) 6.65 (t, J=2.1 Hz, 1 H) 6.92 - 6.97 (m, 2 H) 7.02 - 7.09 (m, 2 H) 7.11 (d, J=2.0 Hz, 1 H) 7.24 (dd, J=9.6, 2.4 Hz, 1 H) 7.37 (d, J=8.3 Hz, 1 H) 8.15 (dd, J=8.8, 5.6 Hz, 1 H) 8.67 (s, 1 H) 12.15 (br s, 1 H)

LC/MS (method LC-A): R_t 1.05 min, MH⁺ 547

[a]_D ²⁰: +139.3° (c 0.435, DMF)

Chiral SFC (method SFC-C): R_t 3.52 min, MH⁺ 547, chiral purity 99.0%.

Enantiomer 1 B:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 3.08 (s, 3 H) 3.72 (s, 3 H) 3.88 - 4.05 (m, 2 H) 4.20 (t, J=4.6 Hz, 2 H) 5.27 (t, J=5.5 Hz, 1 H) 6.37 (d, J=7.7 Hz, 1 H) 6.58 (t, J=1.9 Hz, 1 H) 6.66 (t, J=2A Hz, 1 H) 6.92 - 6.97 (m, 2 H) 7.02 - 7.09 (m, 2 H) 7.11 (d, J=2.0 Hz, 1 H) 7.24 (dd, J=9.6, 2.4 Hz, 1 H) 7.37 (d, J=8.3 Hz, 1 H) 8.15 (dd, J=8.8, 5.6 Hz, 1 H) 8.67 (s, 1 H) 12.15 (br s, 1 H)

LC/MS (method LC-A): R_t 1.05 min, MH⁺ 547

[a]_D ²⁰:-145.6° (c 0.605, DMF)

Chiral SFC (method SFC-C): R_t 4.02 min, MH⁺ 547, chiral purity 97.9%.

-21Example 2: synthesis of 1-(6-chloro-1H-indol-3-yl)-2-(4-chloro-2-(2hydroxyethoxy) phenyl)-2-((3-methoxy-5(methylsulfonyl)phenyl)amino)ethanone (Compound 2) and chiral séparation into Enantiomers 2A and 2B.

Synthesis of intermediate 2a:

Diethylaluminum chloride 1M in hexane (15.0 mL, 15.0 mmol) was added dropwise, at 0°C, to a solution of 6-chloro-1H-indole [CAS 17422-33-2] (1.52 g, 10.0 mmol) in CH₂CI₂ (35 mL). After 30 min at 0°C, a solution of 2-(2-(2(benzyloxy)ethoxy)-4-chlorophenyl)acetyl chloride 1e (5.09 g, 15.0 mmol, synthesis: see Example 1 ) in CH₂CI₂ (10 mL) was added slowly. The reaction mixture was stirred at 0°C for 3 h. 1M Rochelle sait solution was added. The reaction mixture was stirred at room température for 1 h. The formed solids were filtered off and partitioned between EtOAc and 3N HCl. The phases were separated. The aqueous phase was extracted with EtOAc and the combined organic phases were washed with brine, dried over MgSO₄, filtered and concentrated under reduced pressure. The residue was triturated with EtOAc. The precipitate was filtered off and dried under vacuum to yield a first batch of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(6-chloro-1/7-indol-3-yl)ethanone 2a. The filtrate was concentrated under reduced pressure. The residue was purified by flash chromatography on silica gel using a gradient of EtOAc (0% to

50%) in heptane. The fractions containing the desired product were combined

-22and concentrated under reduced pressure. The residue was triturated with EtOAc. The solids were filtered and dried under vacuum to yield a second crop of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1 -(6-chloro-1 /7-indol-3yl)ethanone 2a (overall amount for the two batches: 2.10 g).

Synthesis of intermediate 2b:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(6-chloro-1/7-indol3-yl)ethanone 2a (1.98 g, 4.36 mmol) and 10% palladium on carbon (0.2 g) in EtOAc (40 mL) was stirred at room température for 1 h under H2 atmosphère.

The reaction mixture was diluted with THF and filtered through celite®. The filtrate was concentrated under reduced pressure. The residue was triturated with EtOAc. The solids were filtered off and dried under vacuum to give 1(6-chloro-1/7-indol-3-yl)-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)ethanone 2b (1.29 g).

Synthesis of intermediate 2c:

A solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (1.47 g, 3.91 mmol) in THF (10 mL) was added dropwise, at 0°C, to a solution of 1-(6-chloro-1/7-indol-3-yl)-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)ethanone 2b (1.29 g, 3.55 mmol) in THF (25 mL). The mixture was stirred at room température for 3 h. The precipitate was filtered off and washed with THF. The filtrate was concentrated under reduced pressure to give 2-bromo-1-(6-chloro1H-indol-3-yl)-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)ethanone 2c (1.57 g) which was used in the next step without further purification.

Synthesis of Compound 2 and chiral séparation into Enantiomers 2A and 2B:

A mixture of 2-bromo-1-(6-chloro-1H-indol-3-yl)-2-(4-chloro-2-(2-hydroxyethoxy) phenyl)ethanone 2c (1.57 g, 3.55 mmol) and 3-methoxy-530 (methylsulfonyl)aniline [CAS 62606-02-4] (2.14 g, 10.7 mmol) in CH₃CN (35 mL) and THF (10 mL) was stirred at room température for 5 days. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in EtOAc and washed with 1N HCl. The organic phase was washed with 1N HCl and an aqueous saturated NaHCO₃ solution, dried over Na2SO4, filtered and concentrated under reduced pressure. The residue was triturated with EtOAc and THF. The solids were filtered off. The filtrate was concentrated under reduced pressure and the residue was purified by flash chromatography on silica gel using a gradient of EtOAc (10% to 100%) in CH2CI2. The fractions

-23containing the desired product were combined with the solids previously obtained and concentrated under reduced pressure. The residue was purified by flash chromatography on silica gel using EtOAc (50%) in CH2Cl2as eluent to afford 1-(6-chloro-1/-/-indol-3-yl)-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-25 ((3-methoxy-5-(methylsulfonyl)phenyl)amino)ethanone (Compound 2, 0.99 g) as a racemic mixture.

The chiral séparation of the enantiomers of Compound 2 (894 mg) was performed via Normal Phase Chiral séparation (Stationary phase: AS 20 pm, Mobile phase: 100% methanol). The product fractions were combined and îo evaporated to provide Enantiomer 2A (324 mg) as the first eluted product and

Enantiomer 2B (328 mg) as the second eluted product. Both enantiomers were solidified as follows: the solids were stirred up in a 1/1 mixture of water and MeOH (10 mL) for 1 h, filtered off and dried under vacuum at 50°C to give Enantiomer 2A (253 mg) and Enantiomer 2B (252 mg) as white powders.

Compound 2:

¹H NMR (300 MHz, DMSO-d₆) δ ppm 3.08 (s, 3 H) 3.72 (s, 3 H) 3.83 - 4.10 (m, 2 H) 4.20 (m, 2 H) 5.31 (br. s„ 1 H) 6.38 (d, J=7.9 Hz, 1 H) 6.58 (s, 1 H) 6.66 (s, 1 H) 6.90 - 6.99 (m, 2 H) 7.02 - 7.15 (m, 2 H) 7.23 (dd, J=8.6, 1.6 Hz, 20 1 H) 7.36 (d, J=8.3 Hz, 1 H) 7.50 (s, 1 H) 8.15 (d, J=8.5 Hz, 1 H) 8.70 (s, 1 H)

12.23 (br. s., 1 H)

LC/MS (method LC-E): R_t 1.37 min, MH⁺ 563

Enantiomer 2A:

¹H NMR (360 MHz, DMSO-d₆) δ ppm 3.08 (s, 3 H) 3.72 (s, 3 H) 3.86 - 4.07 (m, 2 H) 4.20 (br t, J=4.5 Hz, 2 H) 5.30 (t, J=5.7 Hz, 1 H) 6.37 (br d, J=7.7 Hz, 1 H) 6.58 (s, 1 H) 6.65 (br s, 1 H) 6.90 - 6.99 (m, 2 H) 7.05 - 7.14 (m, 2 H) 7.22 (dd, J=8.5, 1.9 Hz, 1 H) 7.36 (d, J=8.3 Hz, 1 H) 7.50 (d, J=1.8 Hz, 1 H) 8.15 (d, J=8.5 Hz, 1 H) 8.70 (s, 1 H) 12.23 (s, 1 H)

LC/MS (method LC-A): R_t 1.11 min, MH⁺ 563

[a]_D ²⁰: +149.5° (c 0.43, DMF)

Chiral SFC (method SFC-C): R_t 4.15 min, MH⁺ 563, chiral purity 100%.

Melting point: 122 °C

Enantiomer 2B:

¹H NMR (360 MHz, DMSO-cfe) δ ppm 3.08 (s, 3 H) 3.72 (s, 3 H) 3.86 - 4.07 (m, 2 H) 4.19 (br t, J=4.5 Hz, 2 H) 5.30 (t, J=5.7 Hz, 1 H) 6.37 (br d, J=7.7 Hz,

-241 H) 6.58 (s, 1 H) 6.66 (br s, 1 H) 6.90 - 6.99 (m, 2 H) 7.05 - 7.14 (m, 2 H) 7.23 (dd, J=8.6,1.8 Hz, 1 H) 7.36 (d, J=8.3 Hz, 1 H) 7.50 (d, J=1.9 Hz, 1 H) 8.15 (d, J=8.5 Hz, 1 H) 8.70 (s, 1 H) 12.23 (s, 1 H) LC/MS (method LC-A): R_t 1.11 min, MH* 563

[a]_D ²⁰: -144.1 ° (c 0.401, DMF)

Chiral SFC (method SFC-C): R_t 4.68 min, MH* 563, chiral purity 100%.

Melting point: 121°C

Exemple 3: synthesis of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-methoxyio 1H-indol-3-yl)-2-((3-methoxy-5-(methylsulfonyl)phenyl)amino)ethanone (Compound 3) and chiral séparation into Enantiomers 3A and 3B.

Synthesis of intermediate 3a:

Diethylaluminum chloride 1M in hexane (17.2 mL, 17.2 mmol) was added dropwise, at -50°C, to a suspension of 6-methoxy-1 /-/-indole [CAS 3189-13-7] (1.69 g, 11.5 mmol) in CH₂CI₂ (56 mL). After 30 min at -50°C, a solution of 2-(2(2-(benzyloxy)ethoxy)-4-chlorophenyl)acetyl chloride 1e (5.82 g, 17.2 mmol, synthesis: see Example 1 ) in CH₂CI₂ (23 mL) was slowly added. The reaction mixture was stirred at -50°C for 3 h, was allowed to slowly warm to room température and was stirred at room température overnight. The reaction mixture was diluted with CH₂CI₂and was poured out into a 1M Rochelle sait solution. The mixture was stirred at room température for 2 h. The phases were separated. The organic phase was washed with H₂O and brine, dried over

Na₂SO₄, filtered and concentrated under reduced pressure. The residue was

-25purified by flash chromatography on silica gel using a gradient of EtOAc (20% to 60%) in heptane. The fractions containing desired product were combined and evaporated under reduced pressure. The residue was triturated with EtOAc. The solids were filtered off and dried under vacuum, giving 2-(25 (2-(benzyloxy)ethoxy)-4-chlorophenyl)-1 -(6-methoxy-1 H-indol-3-yl)ethanone 3a (1.39 g).

Synthesis of intermediate 3b:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(6-methoxy-1/-/-indol10 3-yl)ethanone 3a (2.13 g, 4.79 mmol) and 10% palladium on carbon (0.2 g) in

EtOAc (125 mL) was stirred at room température for 5 h under H₂ atmosphère. The reaction mixture was filtered through celite®. The filter pad was washed with THF. The combined filtrâtes were concentrated under reduced pressure. The residue was triturated with CH₂CI₂. The solids were filtered off and dried 15 under vacuum to give 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1 -(6-methoxy1H-indol-3-yl)ethanone 3b (1.38 g).

Synthesis of Compound 3 and chiral séparation into Enantiomers 3A and 3B:

A solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (1.57 g, 4.18 mmol) in THF (27 mL) was added dropwise, at 0°C, to a solution of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-methoxy-1H-indol-3-yl)ethanone 3b (1.38 g, 3.83 mmol) in THF (37 mL). The mixture was stirred at 0°C for 1 h and at room température for 3 h. 3-Methoxy-5-(methylsulfonyl)aniline [CAS

62606-02-4] (3.85 g, 19.1 mmol) was added and the reaction mixture was heated at 60°C for 64 h. The reaction mixture was diluted with EtOAc and washed with 1N HCl. The phases were separated. The organic phase was washed with 1N HCl, an aqueous saturated NaHCO₃ solution, H₂O and brine, dried over Na₂SO4, filtered and concentrated under reduced pressure. The residue was purified by flash chromatography on silica gel using a gradient of MeOH (0% to 10%) in CH₂CI₂. Subséquent purification by flash chromatography on silica gel using a gradient of EtOAc (50% to 95%) in heptane followed by précipitation from EtOAc furnished 2-(4-chloro-2-(2hydroxyethoxy)phenyl)-1-(6-methoxy-1H-indol-3-yl)-2-((3-methoxy-535 (methylsulfonyl)phenyl)amino)ethanone (Compound 3,1.12 g), as a racemic mixture.

The chiral séparation of the enantiomers of Compound 3 (1.12 g) was performed via Normal Phase Chiral séparation (Stationary phase: AS 20 pm,

-26Mobile phase: 100% methanol). The product fractions were combined and evaporated under reduced pressure to provide Enantiomer 3A as the first eluted product and Enantiomer 3B as the second eluted product. Enantiomer 3A was stirred up in a mixture of Et₂O (6 mL) and CH3CN (0.3 mL). The solids were filtered off, washed with Et₂O (5x 1.5 mL) and dried under vacuum to provide Enantiomer 3A (392 mg) as a powder. Enantiomer 3B was stirred up in a mixture of Et₂O (3 mL) and CH₃CN (0.15 mL). The solids were filtered off, washed with Et₂O (5x 1.0 mL) and dried under vacuum to provide Enantiomer 3B (172 mg) as a powder.

Compound 3:

¹H NMR (300 MHz, DMSO-d₆) δ ppm 3.08 (s, 3 H) 3.72 (s, 3 H) 3.76 (s, 3 H) 3.86 - 4.10 (m, 2 H) 4.19 (m, 2 H) 5.30 (t, J=5.3 Hz, 1 H) 6.34 (d, J=7.9 Hz, 1 H) 6.57 (s, 1 H) 6.65 (s, 1 H) 6.83 (dd, J=8.7, 2.0 Hz, 1 H) 6.94 (m, 3 H) 7.06 (d, J=7.7 Hz, 1 H) 7.10 (s, 1 H) 7.36 (d, J=8.6 Hz, 1 H) 8.02 (d, J=8.7 Hz, 1 H)

8.55 (s, 1 H) 11.92 (br. s., 1 H) LC/MS (method LC-D): R_t 3.49 min, MH⁺ 559

Enantiomer 3A:

¹H NMR (360 MHz, DMSO-d₆) δ ppm 3.08 (s, 3 H) 3.72 (s, 3 H) 3.76 (s, 3 H) 3.89 - 4.07 (m, 2 H) 4.19 (t, J=4.7 Hz, 2 H) 5.26 - 5.33 (m, 1 H) 6.34 (d, J=7.8 Hz, 1 H) 6.55 - 6.58 (m, 1 H) 6.63 - 6.67 (m, 1 H) 6.83 (dd, J=8.7, 2.3 Hz, 1 H) 6.91 - 6.97 (m, 3 H) 7.05 (d, J=7.8 Hz, 1 H) 7.11 (d, J=2.Q Hz, 1 H) 7.36 (d, J=8.3 Hz, 1 H) 8.03 (d, J=8.7 Hz, 1 H) 8.55 (s, 1 H) 11.92 (br s, 1 H)

LC/MS (method LC-B): R_t 1.90 min, MH⁺ 559

[a]_D ²⁰: +139.1° (c 0.445, DMF)

Chiral SFC (method SFC-C): R_t4.04 min, MH⁺ 559, chiral purity 100%.

Melting point: 146 °C

Enantiomer 3B:

¹H NMR (360 MHz, DMSO-d₆) δ ppm 3.08 (s, 3 H) 3.72 (s, 3 H) 3.76 (s, 3 H) 3.89 - 4.07 (m, 2 H) 4.19 (t, J=4.7 Hz, 2 H) 5.26 - 5.33 (m, 1 H) 6.35 (d, J=7.7 Hz, 1 H) 6.55 - 6.58 (m, 1 H) 6.63 - 6.67 (m, 1 H) 6.83 (dd, J=8.7, 2.3 Hz, 1 H) 6.91 - 6.97 (m, 3 H) 7.05 (d, J=7.8 Hz, 1 H) 7.11 (d, J=2.0 Hz, 1 H) 7.36 (d, J=8.3 Hz, 1 H) 8.03 (d, J=8.7 Hz, 1 H) 8.55 (s, 1 H) 11.92 (br s, 1 H)

LC/MS (method LC-B): R_t 1.89 min, MH⁺ 559 [a]_D ²⁰:-136.0° (c 0.455, DMF)

Chiral SFC (method SFC-C): R_t4.47 min, MH⁺ 559, chiral purity 100%.

-27Melting point: 149°C

Example 4: synthesis of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1 -(6-fluoro7-methyl-1/-/-indol-3-yl)-2-((3-methoxy-55 (methylsulfonyl)phenyl)amino)ethanone (Compound 4) and chiral séparation

Chiral séparation

Enantiomers 4A and 4

Synthesis of intermediate 4a:

Diethylaluminum chloride 1M in hexane (12.2 mL, 12.2 mmol) was added dropwise, at 0°C, to a suspension of 6-fluoro-7-methyl-1/-/-indole [CAS 5781710-4] (1.20 g, 8.04 mmol) in CH₂CI₂ (17 mL). After 30 min at 0°C, a solution of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)acetyl chloride 1e (4.09 g,

12.1 mmol, synthesis: see Example 1) in CH₂CI₂ (17 mL) was slowly added.

The reaction mixture was stirred at 0°C for 2 h. 1M Rochelle sait solution was added and the mixture was vigorously stirred for 2 h. EtOAc was added. The phases were separated. The aqueous phase was extracted with EtOAc. The organic phases were combined, dried over MgSO₄, filtered and concentrated under reduced pressure. The residue was purified by flash chromatography on silica gel using a gradient of EtOAc (5% to 50%) in CH₂CI₂. Further purification by flash chromatography on silica gel using a gradient of EtOAc (5% to 50%) in heptane furnished 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1 -(6-fluoro-7methyl-1/-/-indol-3-yl)ethanone 4a (2.94 g).

-28Synthesis of intermediate 4b:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(6-fluoro-7-methyl1/7-indol-3-yl)ethanone 4a (2.69 g, 5.95 mmol) and 10% palladium on carbon (0.3 g) in EtOAc (150 mL) was stirred at room température for 2 h under H₂ atmosphère. The reaction mixture was filtered through celite®. The filtrate was concentrated under reduced pressure. The residue was triturated with EtOAc. The solids were filtered off and dried under vacuum to give 2-(4-chloro-2-(2hydroxyethoxy)phenyl)-1-(6-fluoro-7-methyl-1H-indol-3-yl)ethanone 4b (1.15 g).

îo Synthesis of intermediate 4c:

A solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (1.09 g, 2.89 mmol) in THF (18 mL) was added dropwise, at 0°C, to a solution of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-fluoro-7-methyl-1F/-indol-3yl)ethanone 4b (0.95 g, 2.63 mmol) in THF (25 mL). The mixture was stirred at

0°C for 15 min and at room température for 2 h. The precipitate was filtered off and washed with EtOAc. The filtrate was concentrated under reduced pressure to give 2-bromo-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1 -(6-fluoro-7-methyl1H-indol-3-yl)ethanone 4c (1.16 g) which was used in the next step without further purification.

²⁰ .

Synthesis of Compound 4 and chiral séparation into Enantiomers 4A and 4B:

A mixture of 2-bromo-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-fluoro-7methyl-1H-indol-3-yl)ethanone 4c (1.16 g, 2.63 mmol) and 3-methoxy-525 (methylsulfonyl)aniline [CAS 62606-02-4] (1.59 g, 7.90 mmol) in CH₃CN (6 mL) and THF (6 mL) was stirred at room température for 6 days. The reaction mixture was diluted with EtOAc and washed with 1N HCl. The organic phase was washed with 1N HCl, an aqueous saturated NaHCOs solution, H₂O and brine, dried over MgSO4, filtered and concentrated under reduced pressure.

The residue was purified by flash chromatography on silica gel using a gradient of EtOAc (15% to 70%) in CH₂CI₂. The fractions containing the desired product were combined and concentrated under reduced pressure. The residue was triturated with CH₂CI₂. The solids were filtered off and dried under vacuum to give 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1 -(6-fluoro-7-methyl-1 /-/-indol-335 yl)-2-((3-methoxy-5-(methylsulfonyl)phenyl)amino)ethanone (Compound 4, 1.05 g) as a racemic mixture.

The chiral séparation of the enantiomers of Compound 4 (1.37 g) was performed via chiral SFC (Stationary phase: Chiralpak® IA 5 pm 250 x 20 mm,

-29Mobile phase: 36.2% MeOH, 60% CO₂, 3.8% DCM). The product fractions were combined and evaporated under reduced pressure to provide Enantiomer 4A (548 mg) as the first eluted product and Enantiomer 4B (574 mg) as the second eluted product. Enantiomer 4A was stirred up in a mixture of Et₂O (6 mL) and CH3CN (0.25 mL). The solids were filtered off, washed with Et₂O (3x 1.5 mL) and dried under vacuum at 50°C to provide Enantiomer 4A (369 mg) as a powder. Enantiomer 4B was stirred up in a mixture of Et₂O (6 mL) and CH₃CN (0.25 mL). The solids were filtered off, washed with Et₂O (5x 1.0 mL) and dried under vacuum at 50°C to provide Enantiomer 4B (352 mg) as a powder.

Compound 4:

¹H NMR (300 MHz, DMSO-d₆) δ ppm 2.39 (s, 3 H) 3.09 (s, 3 H) 3.72 (s, 3 H) 3.88 - 4.10 (m, 2 H) 4.19 (m, 2 H) 5.32 (br. s., 1 H) 6.40 (d, J=7.7 Hz, 1 H) 6.57 (s, 1 H) 6.67 (s, 1 H) 6.89 - 7.00 (m, 2 H) 7.01 - 7.09 (m, 2 H) 7.11 (d, J=1.8 Hz,

H) 7.36 (d, J=8.3 Hz, 1 H) 7.98 (dd, J=8.5, 5.2 Hz, 1 H) 8.64 (s, 1 H) 12.24 (br. s., 1 H)

LC/MS (method LC-E): R_t 1.31 min, MH⁺ 561

Enantiomer 4A:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 2.38 (s, 3 H) 3.08 (s, 3 H) 3.72 (s, 3 H) 3.90 - 4.07 (m, 2 H) 4.19 (t, J=4.7 Hz, 2 H) 5.28 (brt, J=5.3 Hz, 1 H) 6.40 (d, J=7.8 Hz, 1 H) 6.57 (t, J=1.9 Hz, 1 H) 6.65 - 6.68 (m, 1 H) 6.92 - 6.97 (m, 2 H) 6.98 - 7.06 (m, 2 H) 7.10 (d, J=2.Q Hz, 1 H) 7.36 (d, J=8.3 Hz, 1 H)

7.98 (dd, J=8.7, 5.2 Hz, 1 H) 8.62 (s, 1 H) 12.21 (brs, 1 H)

LC/MS (method LC-A): R_t 1.10 min, MH⁺ 561

[a]_D ²⁰: +116.9° (c 0.575, DMF)

Chiral SFC (method SFC-C): R_t 3.54 min, MH⁺ 561, chiral purity 100%.

Enantiomer 4B:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 2.35 - 2.44 (m, 3 H) 3.09 (s, 3 H) 3.72 (s, 3 H) 3.90 - 4.08 (m, 2 H) 4.20 (t, J=4.7 Hz, 2 H) 5.29 (br s, 1 H) 6.40 (d, J=7.7 Hz, 1 H) 6.56 - 6.60 (m, 1 H) 6.64 - 6.70 (m, 1 H) 6.92 - 6.97 (m, 2 H) 6.98 - 7.07 (m, 2 H) 7.11 (d, J=2.0 Hz, 1 H) 7.36 (d, J=8.3 Hz, 1 H) 7.98 (dd, J=8.8, 5.2 Hz, 1 H) 8.63 (s, 1 H) 12.21 (br s, 1 H)

LC/MS (method LC-B): R_t 2.04 min, MH⁺ 561 [a]_D ²⁰: -115.4° (c 0.455, DMF)

-30Chiral SFC (method SFC-C): R_t4.09 min, MH⁺ 561, chiral purity 100%.

Melting point: 173°C

Example 5: synthesis of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-chloro-75 methyl-1/-/-indol-3-yl)-2-((3-methoxy-5-(methylsulfonyl)phenyl)amino)ethanone (Compound 5) and chiral séparation into Enantiomers 5A and 5B.

OMe

CH₃CN, 70°C 16h

io Synthesis of intermediate 5a:

Diethylaluminum chloride 1M in hexane (8.91 mL, 8.91 mmol) was added dropwise, at 0°C, to a solution of 6-chloro-7-methyl-1/-/-indole [CAS 57817-09-1] (0.984 g, 5.94 mmol) in CH₂CI₂ (40 mL). After 30 min at 0°C, 2-(2-(2(benzyloxy)ethoxy)-4-chlorophenyl)acetyl chloride 1e (2.11 g, 6.22 mmol) in

CH₂CI₂ (40 mL) was added slowly at 0°C. The reaction was stirred at 0°C for 3 h. Ice-water was added and the reaction mixture was extracted with CH₂CI₂. The organic layer was dried over MgSO4, filtered and the solvent was evaporated under reduced pressure. The crude product was purified by flash chromatography on silica gel (15-40 pm, 120 g, heptane/EtOAc 70/30). The pure fractions were combined and evaporated to dryness to give 2-(2(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(6-chloro-7-methyl-1/-/-indol-3yl)ethanone 5a (1.08 g).

-31Synthesis of intermediate 5b:

Under a N₂ flow at 0°C, a solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (0.91 g, 2.42 mmol) in THF (40 mL) was added dropwise to a solution of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(6-chloro-7-methyl-1 H5 indol-3-yl)ethanone 5a (1.08 g, 2.31 mmol) in THF (40 mL). The mixture was stirred at 0°C for 1 h, the cooling bath was removed and stirring was continued at room température for 2.5 h. The precipitate was filtered off and rinsed with EtOAc. The filtrate was concentrated under reduced pressure to give 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-2-bromo-1-(6-chloro-7-methyl-1F/10 indol-3-yl)ethanone 5b (1.3 g).

Synthesis of intermediate 5c:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-2-bromo-1-(6-chloro7-methyl-1H-indol-3-yl)ethanone 5b (1.3 g, 2.38 mmol) and 3-methoxy-515 (methylsulfonyl)aniline [CAS 62606-02-4] (1.43 g, 7.13 mmol) in CH₃CN (80 mL) was stirred at 70°C for 16 h. The solvent was removed under reduced pressure. The crude product was purified by flash chromatography on silica gel (15-40 pm, 24 g, heptane/EtOAc 70/30). The pure fractions were combined and evaporated to dryness to give 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(620 chloro-7-methyl-1 H-indol-3-yl)-2-((3-methoxy-5(methylsulfonyl)phenyl)amino)ethanone 5c (1.1 g).

Synthesis of Compound 5 and chiral séparation of Enantiomers 5A and 5B:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1 -(6-chloro-7-methyl1/7-indol-3-yl)-2-((3-methoxy-5-(methylsulfonyl)phenyl)amino)ethanone 5c (0.8 g, 1.2 mmol) in EtOAc (40 mL) was hydrogenated under atmospheric pressure of H₂ for 12 h with Pd/C (10%) (54 mg, 0.05 mmol) as catalyst. The mixture was filtered through a pad of celite® and washed with EtOAc, and then with CH₂CI₂/CH₃OH 90/10. The filtrate was concentrated under reduced pressure. CH₃CN was added and the solid formed was filtered off and dried to give 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1 -(6-chloro-7-methyl-1 H-indol -3yl)-2-((3-methoxy-5-(methylsulfonyl)phenyl)amino)ethanone (Compound 5) as a racemic mixture.

The enantiomers of Compound 5 (642 mg) were separated via chiral SFC (Stationary phase: Chiralpak® ICOD-H 5 pm 250 x 30 mm, Mobile phase: 60% CO₂, 40% MeOH (+ 0.3% /PrNH₂)) yielding 301 mg of the first eluted enantiomerand 320 mg ofthe second eluted enantiomer. The first eluted

-32enantiomer was crystallized from CH3CN. Tthe precipitate was filtered off and dried to give 198 mg of Enantiomer 5A. The second eluted enantiomer was crystallized from CH3CN. The precipitate was filtered off and dried to give 198 mg of Enantiomer 5B.

Compound 5:

¹H NMR (500 MHz, DMSO-d₆) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.91 - 4.06 (m, 2 H) 4.19 (t, J=4.6 Hz, 2 H) 5.31 (br s, 1 H) 6.41 (d, J=7.6 Hz, 1 H) 6.58 (m, 1 H) 6.67 (br s, 1 H) 6.92 - 6.98 (m, 2 H) 7.06 (d, J=7.9 Hz, 1 H) 7.11 (d, J=î .9 Hz, 1

H) 7.22 (d, J=8.5 Hz, 1 H) 7.35 (d, J=8.2 Hz, 1 H) 7.99 (d, J=8.5 Hz, 1 H) 8.64 (s, 1 H) 12.29 (brs, 1 H)

LC/MS (method LC-C): R_t 3.20 min, MH* 577

Enantiomer 5A:

¹H NMR (500 MHz, DMSO-d₆) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.90 - 4.07 (m, 2 H) 4.19 (t, J=4.6 Hz, 2 H) 5.31 (br s, 1 H) 6.41 (d, J=7.9 Hz, 1 H) 6.58 (t, J=1.9 Hz, 1 H) 6.67 (s, 1 H) 6.93 - 6.96 (m, 2 H) 7.06 (d, J=7.6 Hz, 1 H) 7.11 (d, J=2.2 Hz, 1 H) 7.22 (d, J=8.5 Hz, 1 H) 7.35 (d, J=8.2 Hz, 1 H) 7.99 (d, J=8.5 Hz, 1 H)8.64 (s, 1 H) 12.29 (brs, 1 H)

LC/MS (method LC-C): R_t 3.20 min, MH* 577

[a]_D ²⁰: -119.5° (c 0.37, DMF)

Chiral SFC (method SFC-A): R_t 2.30 min, MH* 577, chiral purity 100%.

Melting point: 207°C

Enantiomer 5B:

¹H NMR (500 MHz, DMSO-de) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.90 - 4.07 (m, 2 H) 4.19 (t, J=4.7 Hz, 2 H) 5.31 (br s, 1 H) 6.41 (d, J=7.9 Hz, 1 H) 6.58 (t, J=1.7 Hz, 1 H) 6.67 (s, 1 H) 6.92 - 6.98 (m, 2 H) 7.07 (d, J=7.9 Hz, 1 H) 7.11 (d, J=1.9 Hz, 1 H) 7.23 (d, J=8.5 Hz, 1 H) 7.36 (d, J=8.5 Hz, 1 H) 7.99 (d, J=8.2

Hz, 1 H) 8.64 (s, 1 H) 12.29 (br s, 1 H)

LC/MS (method LC-C): R₍ 3.20 min, MH* 577

[a]_D ²⁰: +126.1° (c 0.334, DMF)

Chiral SFC (method SFC-A): R_t 2.93 min, MH* 577, chiral purity 99.2%.

Melting point: 206°C

Example 6: synthesis of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-2-((3-methoxy5-(methylsulfonyl)phenyl)amino)-1-(6-methoxy-5-methyl-1/-/-indol-3-yl)ethanone (Compound 6) and chiral séparation into Enantiomers 6A and 6B.

Synthesis of intermediate 6a:

Diethylaluminum chloride 1M in hexane (18.6 mL, 18.6 mmol) was added dropwise, at 0°C, to a solution of 6-methoxy-5-methyl-1/-/-indole [CAS 1071973-95-9] (2 g, 12.4 mmol) in CH2CI2 (30 mL). After 30 min at 0°C, 2-(2-(2(benzyloxy)ethoxy)-4-chlorophenyl)acetyl chloride 1e (4.41 g, 13.0 mmol) in CH2CI2 (30 mL) was added slowly at 0°C. The reaction was stirred at 0°C for

3 h. Ice-water was added and the reaction mixture was extracted with CH2CI2.

The organic layer was dried over MgSO^ filtered and the solvent was evaporated under reduced pressure. The crude product was solidified by trituration with CHsCN/diisopropyl ether. The solid was filtered off and dried to give 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1 -(6-methoxy-5-methyl-1 H15 indol-3-yl)ethanone 6a (2.65 g).

Synthesis of intermediate 6b:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(6-methoxy-5methyl-1/7-indol-3-yl)ethanone 6a (1.7 g, 3.66 mmol) in EtOAc (70 mL) was hydrogenated under an atmospheric pressure of H2 for 12 h with Pd/C (10%) (164 mg, 0.154 mmol) as a catalyst. The mixture was filtered through a pad of celite® and washed with EtOAc. The filtrate was concentrated under reduced pressure to give 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-methoxy-5methyl-1H-indol-3-yl)ethanone 6b (910 mg).

-34Synthesis of Compound 6 and chiral séparation of Enantiomers 6A and 6B:

Under a N₂ flow, at 0°C, a solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (1.09 g, 2.89 mmol) in THF (20 mL) was added dropwise to a solution of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1 -(6-methoxy-5-methyl-1 Hindol-3-yl)ethanone 6b (1.08 g, 2.89 mmol) in THF (30 mL). The mixture was stirred at 0°C for 1 h, the cooling bath was removed and stirring was continued at room température for 3 h. 3-Methoxy-5-(methylsulfonyl)aniline [CAS 6260602-4] (1.74 g, 8.67 mmol) in CH3CN (20 mL) was added dropwise and the resulting mixture was stirred at 55°C for 72 h. The mixture was diluted with CH₂CI₂ and washed with HCl 1N (twice), dried over MgSO4, filtered and the solvent was evaporated under reduced pressure. Purification was performed by flash chromatography on silica gel (15-40 pm, 80 g, CH₂CI₂/CH3OH 98.5/1.5). The pure fractions were combined and evaporated to dryness to give 2-(415 chloro-2-(2-hydroxyethoxy)phenyl)-2-((3-methoxy-5-(methylsulfonyl)phenyl)amino)-1-(6-methoxy-5-methyl-1/-/-indol-3-yl)ethanone (Compound 6, 862 mg) as a racemic mixture.

The enantiomers of Compound 6 (1.3 g) were separated via chiral SFC (Stationary phase: Chiralcel® OD-H 5 pm 250 x 30 mm, Mobile phase: 55%

CO₂, 45% EtOH (+ 0.3% /PrNH₂)). The first eluted enantiomer was solidified from petroleum ether/diisopropyl ether. The precipitate was filtered off and dried to give 441 mg of Enantiomer 6A. The second eluted enantiomer was crystallized from petroleum ether/diisopropyl ether. The precipitate was filtered off and dried to give 461 mg of Enantiomer 6B.

Compound 6:

’H NMR (500 MHz, DMSO-cfe) 2.21 (s, 3 H) 3.08 (s, 3 H) 3.72 (s, 3 H) 3.79 (s, 3 H) 3.89 - 4.06 (m, 2 H) 4.19 (t, J=4.6 Hz, 2 H) 5.30 (br s, 1 H) 6.33 (d, J=7.9 Hz, 1 H) 6.57 (t, J=1.7 Hz, 1 H) 6.65 (br s, 1 H) 6.89 (s, 1 H)

6.92 - 6.96 (m, 2 H) 7.01 (d, J=7.9 Hz, 1 H) 7.10 (d, J=1.9 Hz, 1 H) 7.35 (d, J=8.2 Hz, 1 H) 7.90 (s, 1 H) 8.48 (s, 1 H) 11.84 (br s, 1 H) LC/MS (method LC-C): R_t 2.99 min, MH* 573

Enantiomer 6A:

¹H NMR (500 MHz, DMSO-cfe) δ ppm 2.22 (s, 3 H) 3.09 (s, 3 H) 3.72 (s, 3 H)

3.79 (s, 3 H) 3.90 - 4.07 (m, 2 H) 4.20 (t, J=4A Hz, 2 H) 5.30 (br s, 1 H) 6.34 (d, J=7.6 Hz, 1 H) 6.57 (s, 1 H) 6.65 (br s, 1 H) 6.90 (s, 1 H) 6.93 - 6.97 (m, 2 H)

-357.01 (d, J=7.9 Hz, 1 H) 7.11 (d, 7=1.6 Hz, 1 H) 7.36 (d, 7=8.5 Hz, 1 H) 7.91 (s, 1 H) 8.49 (s, 1 H) 11.84 (br s, 1 H)

LC/MS (method LC-C): R_t 2.98 min, MH* 573 [a]_D ²⁰: +147.1° (c 0.2936, DMF)

Chiral SFC (method SFC-B): R_t 1.86 min, MH* 573, chiral purity 100%.

Enantiomer 6B:

¹H NMR (500 MHz, DMSO-d₆) δ ppm 2.22 (s, 3 H) 3.09 (s, 3 H) 3.72 (s, 3 H) 3.79 (s, 3 H) 3.90 - 4.07 (m, 2 H) 4.20 (br t, 7=4.4 Hz, 2 H) 5.30 (br s, 1 H) 6.34 (d, 7=7.6 Hz, 1 H) 6.57 (s, 1 H) 6.65 (br s, 1 H) 6.90 (s, 1 H) 6.92 - 6.97 (m, 2 H)

7.02 (d, 7=7.6 Hz, 1 H) 7.11 (d, 7=1.6 Hz, 1 H) 7.36 (d, 7=8.5 Hz, 1 H) 7.91 (s, 1 H) 8.49 (s, 1 H) 11.84 (brs, 1 H) LC/MS (method LC-C): R_t 2.98 min, MH* 573 [a]_D ²⁰: -152.4° (c 0.2927, DMF)

Chiral SFC (method SFC-B): R_t 3.43 min, MH* 573, chiral purity 100%.

Example 7: synthesis of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(5,6-difluoro1H-indol-3-yl)-2-((3-methoxy-5-(methylsulfonyl)phenyl)amino)ethanone (Compound 7) and chiral séparation into Enantiomers 7A and 7B.

7c

-36Synthesis of intermediate 7a:

Diethylaluminum chloride 1M in hexane (12.5 mL, 12.5 mmol) was added dropwise, at 0°C, to a solution of 5,6-difluoro-1/7-indole [CAS 169674-01-5] (1.27 g, 8.30 mmol) in CH₂CI₂ (50 mL). After 30 min at 0°C, a solution of 2-(2(2-(benzyloxy)ethoxy)-4-chlorophenyl)acetyl chloride 1e (4.23 g, 12.5 mmol, synthesis: see Example 1) in CH₂CI₂ (20 mL) was slowly added. The reaction mixture was stirred at 0°C for 3 h. 1M Rochelle sait solution was added and the mixture was stirred vigorously for 30 min. H₂O was added and the phases were separated. The aqueous phase was extracted twice with EtOAc. The organic phases were combined, washed with brine, dried over MgSO4, filtered and concentrated under reduced pressure. The residue was triturated with Et₂O. The solids were filtered off and dried under vacuum to give 2-(2-(2(benzyloxy)ethoxy)-4-chlorophenyl)-1 -(5,6-difluoro-1 /-/-indol-3-yl)ethanone 7a (1.37 g).

Synthesis of intermediate 7b:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(5,6-difluoro-1/-/indol-3-yl)ethanone 7a (1.43 g, 3.14 mmol) and 10% palladium on carbon (0.07 g) in EtOAc (70 mL) was stirred at room température for 45 min under H₂atmosphère. The reaction mixture was filtered through celite®. The filtrate was concentrated under reduced pressure. The residue was purified by flash chromatography on silica gel using a gradient of EtOAc (0% to 15%) in CH₂CI₂to provide 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1 -(5,6-difluoro-1 /-/-indol-3yl)ethanone 7b (0.88 g).

Synthesis of intermediate 7c:

A solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (1.68 g, 4.47 mmol) in THF (20 mL) was added dropwise to a solution of 2-(4-chloro-2(2-hydroxyethoxy)phenyl)-1-(5,6-difluoro-1/-/-indol-3-yl)ethanone 7b (1.49 g, 4.07 mmol) in THF (45 mL). The mixture was stirred at room température for 4 h. The precipitate was filtered off and washed with EtOAc. The filtrate was concentrated under reduced pressure to give 2-bromo-2-(4-chloro-2-(2hydroxyethoxy)phenyl)-1-(5,6-difluoro-1H-indol-3-yl)ethanone 7c (1.81 g) which was used in the next step without further purification.

-37Synthesis of Compound 7 and chiral séparation into Enantiomers 7A and 7B:

A mixture of 2-bromo-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(5,6-difluoro1/-/-indol-3-yl)ethanone 7c (1.81 g, 4.07 mmol) and 3-methoxy-5(methylsulfonyl)aniline [CAS 62606-02-4] (2.46 g, 12.2 mmol) in CH₃CN (40 mL) was stirred at room température for 3 d. The reaction mixture was concentrated under reduced pressure. The residue was partitioned between EtOAc and 1N HCl. The phases were separated. The aqueous phase was extracted with EtOAc. The organic phases were combined, washed with brine, dried over MgSO4, filtered and concentrated under reduced pressure. The residue was purified by flash chromatography on silica gel using a gradient of EtOAc (50% to 100%) in heptane. The fractions containing the desired product were combined and concentrated under reduced pressure. The residue was triturated with CH2CI2. The solids were filtered off and dried under vacuum to give 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(5,6-difluoro-1/-/-indol-3-yl)-2-((3methoxy-5-(methylsulfonyl)phenyl)amino)ethanone (Compound 7, 0.97 g) as a racemic mixture.

The chiral séparation of the enantiomers of Compound 7 (914 mg) was performed via Normal Phase Chiral séparation (Stationary phase: AS 20 pm, Mobile phase: 100% methanol). The product fractions were combined and evaporated to provide Enantiomer 7A (351 mg) as the first eluted product and Enantiomer 7B (337 mg) as the second eluted product. Enantiomer 7A was stirred up in a 1/1 mixture of MeOH and water (10 mL). The solids were filtered off, washed with a small amount of MeOH/water 1/1 and dried under vacuum at 50°C to provide Enantiomer 7A (323 mg) as a white powder. Enantiomer 7B was further purified via préparative chiral SFC (Stationary phase: Chiralpak® Diacel AS 20 x 250 mm, Mobile phase: CO₂, EtOH (+ 0.4% /PrNH₂)). The fractions containing desired product were combined and evaporated under reduced pressure. The residue was stirred up in a 1/1 mixture of MeOH and water (10 mL) for 30 min. The solids were filtered off, washed with a small amount of MeOH/water 1/2 and dried under vacuum at 50°C to provide Enantiomer 7B (209 mg) as a white powder.

Compound 7:

’H NMR (300 MHz, DMSO-d₆) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.86 - 4.07 (m, 2 H) 4.19 (m, 2 H) 5.29 (t, J=5.3 Hz, 1 H) 6.37 (d, J=7.9 Hz, 1 H) 6.58 (s, 1 H) 6.65 (s, 1 H) 6.91 - 7.01 (m, 2 H) 7.05 - 7.16 (m,.2 H) 7.36 (d, J=8.3 Hz,

-381 H) 7.50 (dd, 7=10.7, 7.0 Hz, 1 H) 8.01 (dd, 7=11.3, 8.3 Hz, 1 H) 8.72 (s, 1 H) 12.31 (br. s., 1 H)

LC/MS (method LC-E): R_t 1.21 min, MH* 565

Enantiomer 7A:

¹H NMR (360 MHz, DMSO-d₆) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.87 - 4.05 (m, 2 H) 4.19 (br t, 7=4.6 Hz, 2 H) 5.31 (br t, 7=5.4 Hz, 1 H) 6.37 (d, 7=7.8 Hz, 1 H) 6.58 (br s, 1 H) 6.65 (br s, 1 H) 6.92 - 7.01 (m, 2 H) 7.06 - 7.16 (m, 2 H) 7.36 (d, 7=8.3 Hz, 1 H) 7.50 (dd, 7=10.7, 6.9 Hz, 1 H) 8.01 (dd, 7=11.1,8.1 Hz, 1 H) 8.72 (s, 1 H) 12.31 (brs, 1 H)

LC/MS (method LC-A): R_t 1.15 min, MH* 565

[a]_D ²⁰: +120.2° (c 0.499, DMF)

Chiral SFC (method SFC-C): R_t 3.47 min, MH* 565, chiral purity 100%.

Enantiomer 7B:

¹H NMR (360 MHz, DMSO-7₆) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.86 - 4.06 (m, 2 H) 4.19 (br t, 7=4.5 Hz, 2 H) 5.30 (br t, 7=5.4 Hz, 1 H) 6.37 (d, 7=7.8 Hz, 1 H) 6.58 (S, 1 H) 6.65 (s, 1 H) 6.92 - 7.00 (m, 2 H) 7.06 - 7.14 (m, 2 H) 7.36 (d, 7=8.3 Hz, 1 H) 7.50 (dd, 7=10.7, 6.8 Hz, 1 H) 8.01 (dd, 7=11.1,8.0 Hz, 1 H) 8.72 (s, 1 H) 12.30 (s, 1 H)

LC/MS (method LC-A): R_t 1.10 min, MH* 565

[a]_D ²⁰:-125.0° (c 0.414, DMF)

Chiral SFC (method SFC-D): R_t 1.60 min, MH* 565, chiral purity 100%.

Exemple 8: synthesis of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-fluoro5-methyl-1/7-indol-3-yl)-2-((3-methoxy-5(methylsulfonyl)phenyl)amino)ethanone (Compound 8) and chiral séparation into Enantiomers 8A and 8B.

ci

8c

OMe

DIPEA

CH₃CN, 55°C,18h

Chiral séparation

Enantiomers 8A and 8E

Synthesis of intermediate 8a:

Diethylaluminum chloride 1M in hexane (17.0 mL, 17.0 mmol) was added dropwise, at 0°C and under N2-atmosphere, to a solution of 6-fluoro-5-methyl1H-indole [CAS 162100-95-0] (1.69 g, 11.3 mmol) in CH₂CI₂(150 mL). After 15 min at 0°C, a solution of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)acetyl chloride 1e (5.37 g, 15.8 mmol, synthesis: see Example 1) in CH₂CI₂ (100 mL) ίο was slowly added. The reaction mixture was stirred at 0°C for 1 h and at room température for 2 h. The reaction mixture was poured out into an ice/Rochelle sait solution and the mixture was vigorously stirred. The layers were separated. The organic layer was dried over MgSO₄ and filtered over a short pad of dicalite®. The filter cake was rinsed a few times with THF and the combined filtrâtes were concentrated under reduced pressure. The solid residue was suspended in CH₃CN (20 mL), filtered off, washed with a small amount of CH₃CN, and dried under vacuum at 50 °C to give 2-(2-(2-(benzyloxy)ethoxy)-4chlorophenyl)-1-(6-fluoro-5-methyl-1H-indol-3-yl)ethanone 8a (2.39 g) as a white solid.

Synthesis of intermediate 8b:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(6-fluoro-5-methyl1/7-indol-3-yl)ethanone 8a (2.39 g, 4.71 mmol) and 10% palladium on carbon (0.5 g) in EtOAc (135 mL) and THF (15 mL) was stirred at room température for

-4015 min under H₂ atmosphère. The reaction mixture was filtered through dicalite® and the filter cake was washed with EtOAc and THF. The combined filtrâtes were concentrated under reduced pressure. The residue was stirred up in DIPE/THF (2/1), filtered off, washed with DIPE (3x) and dried under vacuum 5 at 50°C to provide 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1 -(6-fluoro-5-methyl1/-/-indol-3-yl)ethanone 8b (0.90 g).

Synthesis of intermediate 8c:

Phenyltrimethylammonium tribromide [CAS 4207-56-1] (982 mg, 2.61 mmol) ίο was added to a cooled (0°C) solution of 2-(4-chloro-2-(2hydroxyethoxy)phenyl)-1-(6-fluoro-5-methyl-1H-indol-3-yl)ethanone 8b (900 mg, 2.49 mmol) in THF (60 mL), under N₂-atmosphere. The reaction mixture was stirred at 0°C for 90 min and at room température for 30 min. The precipitate was filtered off and washed with THF (2x). The combined filtrâtes were concentrated under reduced pressure to give 2-bromo-2-(4-chloro-2-(2hydroxyethoxy)phenyl)-1-(6-fluoro-5-methyl-1H-indol-3-yl)ethanone 8c (1.1 g) which was used in the next step without further purification.

Synthesis of Compound 8 and chiral séparation into Enantiomers 8A and 20 8B:

A mixture of 2-bromo-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(6-fluoro-5methyl-1H-indol-3-yl)ethanone 8c (1.10 g, 2.49 mmol), 3-methoxy-5(methylsulfonyl)aniline [CAS 62606-02-4] (1.00 g, 4.97 mmol) and diisopropylethylamine (857 pL, 4.97 mmol) in CH3CN (60 mL) was stirred at 25 55°C for 18 h. The reaction mixture was cooled to room température and poured out into stirring water (250 mL). The product was extracted with a mixture of Et₂O/2-MeTHF 9/1, and with Et₂O. The combined organic layers were washed with brine, dried over MgSO4, filtered, and evaporated under reduced pressure. The residue was purified by flash chromatography (Stationary phase: Grâce Reveleris® silica 40 g, Mobile phase: heptane/EtOAc/EtOH gradient 100/0/0 to 40/45/15). The desired fractions were combined and evaporated under reduced pressure. The residue was purified via préparative HPLC (Stationary phase: RP XBridge® Prep C18 OBD - 10 pm, 50 x 150 mm, Mobile phase: 0.25% NH4HCO3 solution in water, CH₃CN). The 35 desired fractions were combined and evaporated under reduced pressure and the solid residue was dried at 50°C under vacuum to give racemic 2-(4-chloro2-(2-hydroxyethoxy)phenyl)-1-(6-fluoro-5-methyl-1//-indol-3-yl)-2-((3-methoxy5-(methylsulfonyl)phenyl)amino)ethanone (Compound 8, 600 mg).

-41The chiral séparation of the enantiomers of Compound 8 (570 mg) was performed via Normal Phase Chiral séparation (Stationary phase: AS 20 pm, Mobile phase: 100% methanol). The product fractions were combined and evaporated to provide Enantiomer 8A as the first eluted product and

Enantiomer 8B as the second eluted product. Both enantiomers were stirred up in water/MeOH 4/1 (5 mL), filtered off, washed with water/MeOH 4/1 and dried under vacuum at 50°C to provide Enantiomer 8A (178 mg) and Enantiomer 8B (189 mg).

îo Compound 8:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 2.30 (d, 7=1.3 Hz, 3 H) 3.08 (s, 3 H) 3.72 (s, 3 H) 3.88 - 4.05 (m, 2 H) 4.19 (t, 7=4.5 Hz, 2 H) 5.27 (br s, 1 H) 6.35 (d, 7=7.7 Hz, 1 H) 6.57 (t, 7=1.9 Hz, 1 H) 6.64 (t, 7=1.5 Hz, 1 H) 6.92 - 6.97 (m, 2 H) 7.01 (d, 7=7.7 Hz, 1 H) 7.10 (d, 7=2.0 Hz, 1 H) 7.19 (d, 7=10.1 Hz, 1 H)

7.36 (d, 7=8.4 Hz, 1 H) 8.02 (d, 7=7.9 Hz, 1 H) 8.61 (s, 1 H) 12.02 (brs, 1 H)

LC/MS (method LC-B): R_t 2.01 min, MH* 561

Enantiomer 8A:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 2.30 (d, 7=1.3 Hz, 3 H) 3.08 (s, 3 H) 3.72 20 (s, 3 H) 3.87 - 4.06 (m, 2 H) 4.19 (t, 7=4.5 Hz, 2 H) 5.26 (br t, 7=5.1 Hz, 1 H)

6.35 (d, 7=7.7 Hz, 1 H) 6.57 (t, 7=1.8 Hz, 1 H) 6.65 (t, 7=1.9 Hz, 1 H) 6.92 - 6.97 (m, 2 H) 7.01 (d, 7=7.7 Hz, 1 H) 7.10 (d, 7=1.8 Hz, 1 H) 7.19 (d, 7=10.1 Hz, 1 H) 7.36 (d, 7=8.1 Hz, 1 H) 8.03 (d, 7=7.9 Hz, 1 H) 8.61 (s, 1 H) 12.03 (brs, 1 H)

LC/MS (method LC-A): R_t 1.10 min, MH* 561

[a]_D ²⁰: +172.4° (c 0.485, DMF)

Chiral SFC (method SFC-C): R_t 3.59 min, MH* 561, chiral purity 100%.

Enantiomer 8B:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 2.30 (d, 7=1.3 Hz, 3 H) 3.08 (s, 3 H) 3.72 (s, 3 H) 3.88 - 4.05 (m, 2 H) 4.19 (t, 7=4.6 Hz, 2 H) 5.27 (br s, 1 H) 6.35 (d, 7=7.7 Hz, 1 H) 6.57 (t, 7=1.8 Hz, 1 H) 6.65 (t, 7=1.8 Hz, 1 H) 6.91 - 6.97 (m, 2 H) 7.01 (d, 7=7.9 Hz, 1 H) 7.10 (d, 7=2.0 Hz, 1 H) 7.19 (d, 7=10.3 Hz, 1 H) 7.36 (d, 7=8.4 Hz, 1 H) 8.03 (d, 7=7.7 Hz, 1 H) 8.61 (s, 1 H) 11.94 (br s, 1 H)

LC/MS (method LC-A): R_t 1.10 min, MH* 561

[a]_D ²⁰: -170.6° (c 0.425, DMF)

Chiral SFC (method SFC-C): R_t 4.06 min, MH* 561, chiral purity 98.7%.

-42Example 9: synthesis of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-2-((3-methoxy5-(methylsulfonyl)phenyl)amino)-1-(5-(trifluoromethyl)-1H-indol-3-yl)ethanone (Compound 9) and chiral séparation into Enantiomers 9A and 9B.

i ! I i

Et₃N, EtOH

1)70°C 12h

2) MW: 160°C 4 min

Chiral séparation

Enantiomers 9A and 9 B

Synthesis of the intermediate 9a:

A mixture of 4-chloro-2-hydroxy-benzaldehyde [CAS 2420-26-0] (7.72 g, 49.31 mmol), benzyl 2-bromoethyl ether [CAS 1462-37-9] (7.8 mL, 49.31 mmol) and potassium carbonate (8.2 g, 59.17 mmol) in CH3CN (200 mL) was heated under reflux for 12 h. The mixture was evaporated under reduced pressure. The residue was taken up with EtOAc, washed with water (twice), dried over

MgSO4, filtered and the solvent was evaporated to afford 2-(2(benzyloxy)ethoxy)-4-chlorobenzaldehyde 9a (14.2 g).

Synthesis of the intermediate 9b:

A mixture of 2-(2-(benzyloxy)ethoxy)-4-chlorobenzaldehyde 9a (2.1 g,

7.22 mmol), 3-methoxy-5-(methylsulfonyl)aniline [CAS 62606-02-4] (1.45 g,

-437.22 mmol) in EtOH (18 mL) was stirred at 60°C for 6 h. The resulting solution containing imine 9b was used as such in the next step.

Synthesis of the intermediate 9c:

To a solution of 3-benzyl-5-(2-hydroxyethyl)-4-methylthiazol-3-ium chloride [CAS 4568-71-2] (1.95 g, 7.22 mmol) in EtOH (10 mL) was added triethylamine (1 mL, 7.22 mmol) and the resulting mixture was stirred at 70°C for 10 min. This solution was added to a stirred mixture of imine 9b (3.42 g, 7.22 mmol, solution in EtOH, see above: synthesis of intermediate 9b) and tert-butyl

3-formyl-5-(trifluoromethyl)-1H-indole-1-carboxylate [CAS 1493799-60-2] (2.7 g, 8.67 mmol) at room température. The mixture was stirred at 70°C for 12 h. The reaction mixture was cooled down to room température and transferred into a seaied tube that was subsequently heated at 160°C using one single mode microwave (Biotage InitiatorEXP 60 ) with a power output ranging from 0 to

400 W for 4 min (fixed hold time). The mixture was evaporated under reduced pressure. Purification was performed by flash chromatography on silica gel (15-40 pm, 120 g, eluent: CH₂CI₂/CH₃OH 99.5/0.5).The pure fractions were combined and evaporated under reduced pressure. The residue (3.48 g) was taken up with CH₃OH and stirred at room température for 1 h. The precipitate was filtered off and dried to yield 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-2((3-methoxy-5-(methylsulfonyl)phenyl)amino)-1-(5-(trifluoromethyl)-1/-/-indol-3yl)ethanone 9c (1.23 g).

Synthesis of Compound 9 and chiral séparation into Enantiomers 9A and

9B:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-2-((3-methoxy-5(methylsulfonyl)phenyl)amino)-1 -(5-(trifluoromethyl)-1 H-indol-3-yl)ethanone 9d (1.10 g, 1.60 mmol) in EtOAc (20 mL) was hydrogenated for 10 min under atmospheric pressure of H₂ with 10% Pd/C (340 mg, 0.32 mmol) as the catalyst.

The reaction was diluted with EtOAc and filtered through a pad of celite®. The filtrate was evaporated under reduced pressure to afford 2-(4-chloro-2-(2hydroxyethoxy)phenyl)-2-((3-methoxy-5-(methylsulfonyl)phenyl)amino)-1-(5(trifluoromethyl)-1/7-indol-3-yl)ethanone (compound 9, 910 mg) as a racemic mixture.

The enantiomers of Compound 9(1.15 g) were separated via chiral SFC (Stationary phase: Chiralpak® IC 5 pm 250 x 30 mm, Mobile phase: 75% CO₂, 25% EtOH + 0.3% iPrNH₂) yielding 544 mg of the first eluted enantiomer and 464 mg of the second eluted enantiomer. The first eluted enantiomer was

-44crystallized from CH3OH and water. The precipitate was filtered off and dried to give 362 mg of Enantiomer 9A. The second eluted enantiomer was crystallized from CH3OH and water. The precipitate was filtered off and dried to give 348 mg of Enantiomer 9B.

Compound 9:

¹H NMR (500 MHz, DMSO-d₆) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.87 - 4.09 (m, 2 H) 4.20 (t, J=4.6 Hz, 2 H) 5.32 (t, J=5.5 Hz, 1 H) 6.42 (d, J=7.9 Hz, 1 H)

6.59 (t, J=1.9 Hz, 1 H) 6.66 (s, 1 H) 6.94 - 6.98 (m, 2 H) 7.10 (d, J=7.9 Hz, 1 H)

7.12 (d, J=1.9 Hz, 1 H) 7.38 (d, J=8.2 Hz, 1 H) 7.54 (dd, J=8.7,1.7 Hz, 1 H)

7.67 (d, J=8.5 Hz, 1 H) 8.50 (s, 1 H) 8.83 (s, 1 H) 12.53 (br s, 1 H)

LC/MS (method LC-C): R_t 3.12 min, MH* 597

Melting point: 228°C

Enantiomer 9A:

¹H NMR (500 MHz, DMSO-d₆) δ ppm 3.09 (s, 3 H) 3.73 (s, 3 H) 3.87 - 4.07 (m, 2 H) 4.20 (t, J=4.4 Hz, 2 H) 5.32 (br t, J=4.4 Hz, 1 H) 6.42 (d, J=7.9 Hz, 1 H) 6.59 (S, 1 H) 6.66 (brs, 1 H) 6.94 - 6.99 (m, 2 H) 7.10 (d, J=7.9 Hz, 1 H) 7.12 (d, J=1.3 Hz, 1 H) 7.38 (d, J=8.2 Hz, 1 H) 7.54 (d, J=8.5 Hz, 1 H) 7.67 (d, J=8.5 20 Hz, 1 H) 8.50 (s, 1 H) 8.83 (s, 1 H) 12.52 (br s, 1 H)

LC/MS (method LC-C): R_t 3.12 min, MH* 597

[a]_D ²⁰:-154.3° (c 0.245, DMF)

Chiral SFC (method SFC-E): R_t 1.75 min, MH* 597, chiral purity 100%.

Enantiomer 9B:

¹H NMR (500 MHz, DMSO-d₆) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.88 - 4.06 (m, 2 H) 4.20 (t, J=4.4 Hz, 2 H) 5.32 (br t, J=4.7 Hz, 1 H) 6.42 (d, J=7.9 Hz, 1 H)

6.59 (s, 1 H) 6.66 (br s, 1 H) 6.94 - 6.98 (m, 2 H) 7.10 (d, J=7.9 Hz, 1 H) 7.12 (d, J=1.6 Hz, 1 H) 7.38 (d, J=8.2 Hz, 1 H) 7.54 (d, J=8.5 Hz, 1 H) 7.67 (d, J=8.5

Hz, 1 H) 8.50 (s, 1 H) 8.83 (s, 1 H) 12.50 (br s, 1 H)

LC/MS (method LC-C): R_t 3.12 min, MH* 597

[a]_D ²⁰: +142.6° (c 0.284, DMF)

Chiral SFC (method SFC-E): R_t 2.15 min, MH* 597, chiral purity 100%.

Example 10: synthesis of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-2-((3methoxy-5-(methylsulfonyl)phenyl)amino)-1-(5-(tnfluoromethoxy)-1/7-indol-3yl)ethanone (Compound 10) and chiral séparation into Enantiomers 10Aand 10B.

Enantiomers 10A and 1

Synthesis of intermediate 10a:

Diethylaluminum chloride 1M in hexane (18.2 mL, 18.2 mmol) was added dropwise, at 0°C and under N₂-atmosphere, to a solution of 5(trifluoromethoxy)-l/-/-indole [CAS 262593-63-5] (2.44 g, 12.1 mmol) in CH₂CI₂(150 mL). After stirring for 15 min at 0°C, a solution of 2-(2-(2(benzyloxy)ethoxy)-4-chlorophenyl)acetyl chloride 1e (6.17 g, 18.2 mmol, synthesis: see Example 1 ) in CH₂CI₂ (100 mL) was slowly added. The reaction mixture was stirred at 0°C for 90 min and at room température for 2 h. The reaction mixture was cooled to 0°C and a solution of Rochelle sait [CAS 610016-9] (6.85 g, 24.3 mmol) in water (7 mL) was added dropwise. The mixture was vigorously stirred for 30 min at 0°C. The ice-bath was removed and THF (200 mL) was added. After stirring for 30 min at room température, Na₂SO4 (25 g) was added. The mixture was stirred for 90 min and filtered over dicalite®. The filter cake was washed with THF (4x 150 mL) and the combined filtrâtes were evaporated under reduced pressure and co-evaporated to dryness with a mixture CH3CN and toluene. The solid residue was stirred up in a mixture of toluene (5 mL) and CH3CN (2.5 mL), filtered off, washed with a small amount of toluene/CHsCN (2/1 ), and dried under vacuum at 50°C to give 2-(2-(2(benzyloxy)ethoxy)-4-chlorophenyl)-1-(5-(trifluoromethoxy)-1/V-indol-3yl)ethanone 10a (1.89 g). The filtrate was evaporated under reduced pressure. The residue (6.8 g) was purified by flash chromatography (Biotage® SNAP

Ultra silica 100 g, eluent: heptane/EtOAc/EtOH gradient 100/0/0 to 40/45/15).

-46The desired fractions were combined and evaporated under reduced pressure, and co-evaporated with EtOAc. The product was stirred up in a mixture of DIPE (15 mL) and EtOAc (1 mL), filtered off, washed with DIPE (2x), and dried at 50°C under vacuum to provide a second batch of 10a (1.62 g).

Synthesis of intermediate 10b:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(5-(trifluoromethoxy)1H-indol-3-yl)ethanone 10a (1.62 g, 3.22 mmol) and 10% palladium on carbon (0.5 g) in EtOAc (75 mL) and THF (10 mL) was stirred at room température for 20 min under H₂ atmosphère. The reaction mixture was filtered through dicalite® and the filter cake was washed with THF. The combined filtrâtes were concentrated under reduced pressure. The solid residue was combined with another fraction (total amount: 3 g), stirred up in CH₂CI₂ (8 mL), filtered off, washed with CH₂CI₂ (5x 1 mL) and dried under vacuum at 45°C to provide 2-(4chloro-2-(2-hydroxyethoxy)phenyl)-1-(5-(trifluoromethoxy)-1/7-indol-3yl)ethanone 10b (1.64 g).

Synthesis of intermediate 10c:

Phenyltrimethylammonium tribromide [CAS 4207-56-1] (1.56 g, 4.16 mmol) was added portionwise to a cooled (0°C) solution of 2-(4-chloro-2-(2hydroxyethoxy)phenyl)-1 -(5-(trifluoromethoxy)-1 /7-indol-3-yl)ethanone 10b (1.64 g, 3.96 mmol) in THF (75 mL), under N₂-atmosphere. The reaction mixture was stirred at 0^eC for 1 h and at room température for 40 min. The precipitate was filtered off and washed with THF (2x). The combined filtrâtes were concentrated under reduced pressure to give 2-bromo-2-(4-chloro-2-(2hydroxyethoxy)phenyl)-1 -(5-(trifluoromethoxy)-1 /7-indol-3-yl)ethanone 10c (1.92 g) which was used in the next step without further purification.

Synthesis of Compound 10 and chiral séparation into Enantiomers 10A and 10B:

A mixture of 2-bromo-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(5(trifluoromethoxy)-1/7-indol-3-yl)ethanone 10c (1.95 g, 3.96 mmol), 3-methoxy5-(methylsulfonyl)aniline [CAS 62606-02-4] (1.60 g, 7.92 mmol) and diisopropylethylamine (1.37 mL, 7.92 mmol) in CH₃CN (75 mL) was stirred at 55°C for 18 h and at room température for 24 h. The reaction mixture was cooled to room température and poured out into stirring water (350 mL). The product was extracted with Et₂O (2x). The combined organic layers were washed with brine, dried over MgSO4, filtered, and evaporated under reduced

-47pressure. The residue was purified by flash chromatography (Stationary phase: Grâce Reveleris® silica 80 g, Mobile phase: heptane/EtOAc/EtOH gradient 100/0/0 to 40/45/15). The desired fractions were combined, evaporated under reduced pressure and co-evaporated with CH3CN. The residue was purified via préparative HPLC (Stationary phase: RP XBridge® Prep C18 OBD -10 pm, 50 x 150 mm, Mobile phase: 0.25% NH4HCO3 solution in water, CH3CN). The desired fractions were combined, evaporated under reduced pressure, co-evaporated with MeOH and was dried at 50°C under vacuum to give racem ic 2-(4-ch loro-2-(2-hyd roxyethoxy )phenyl )-2-((3-methoxy-510 (methylsulfonyl)phenyl)amino)-1-(5-(trifluoromethoxy)-1/-/-indol-3-yl)ethanone (Compound 10, 700 mg). A small sample of Compound 10 (50 mg) was solidified by slow évaporation from a solution in MeOH/water using a Rotavapor. The solids were filtered off, washed with water (3x) and dried at 45°C under vacuum to provide an analytical sample of Compound 10 (46 mg).

The chiral séparation of the Enantiomers of Compound 10 (650 mg) was performed via Normal Phase Chiral séparation (Stationary phase: Whelk-O1 (R,R), Mobile phase: 20% éthanol, 80% heptane). The product fractions were combined and evaporated to provide Enantiomer 10A as the first eluted product and Enantiomer 10B as the second eluted product. Both enantiomers were stirred up in a mixture of water (4 mL) and MeOH (1.25 mL), filtered off, washed with water/MeOH 4/1 (4x) and dried under vacuum at 45°C to provide Enantiomer 10A (115 mg) and Enantiomer 10B (140 mg).

Compound 10:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 3.08 (s, 3 H) 3.72 (s, 3 H) 3.87 - 4.05 (m, 2 H) 4.20 (t, J=4.Q Hz, 2 H) 5.27 (t, J=5.6 Hz, 1 H) 6.38 (d, J=7.9 Hz, 1 H) 6.58 (t, J=1.8 Hz, 1 H) 6.65 (t, J=2.4 Hz, 1 H) 6.92 - 6.98 (m, 2 H) 7.07 (d, J=7.7 Hz, 1 H) 7.12 (d, J=2.0 Hz, 1 H) 7.21 (dd, J=8.8,1.8 Hz, 1 H) 7.38 (d, J=8.1 Hz, 1 H) 7.56 (d, J=8.8 Hz, 1 H) 8.07 (d, J=1.1 Hz, 1 H) 8.77 (s, 1 H)

12.37 (s, 1 H)

LC/MS (method LC-A): R_t 1.15 min, MH⁺ 613

Enantiomer 10A:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.87 - 4.07 (m, 2 H) 4.20 (br t, J=4.4 Hz, 2 H) 5.29 (br t, J=5.4 Hz, 1 H) 6.39 (d, J=7.7 Hz,

H) 6.59 (t, J=1.9 Hz, 1 H) 6.65 (t, J=2.2 Hz, 1 H) 6.91 - 7.00 (m, 2 H) 7.09 (d, J=7.9 Hz, 1 H) 7.12 (d, J=1.8 Hz, 1 H) 7.21 (dd, J=8.7,1.7 Hz, 1 H) 7.38

-48(d, 7=8.4 Hz, 1 H) 7.57 (d, J=8.8 Hz, 1 H) 8.08 (br s, 1 H) 8.77 (s, 1 H) 12.38 (brs, 1 H)

LC/MS (method LC-A): R_t 1.15 min, MH* 613

[a]_D ²⁰: -139.3° (c 0.425, DMF)

Chiral SFC (method SFC-C): R_t 3.27 min, MH* 613, chiral purity 100%.

Enantiomer 10B:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.88 - 4.05 (m, 2 H) 4.20 (t, 7=4.5 Hz, 2 H) 5.29 (t, 7=5.5 Hz, 1 H) 6.39 (d, 7=7.7 Hz, 1 H)

6.58 (t, 7=1.8 Hz, 1 H) 6.65 (t, 7=1.9 Hz, 1 H) 6.92 - 6.99 (m, 2 H) 7.09 (d, 7=7.7 Hz, 1 H) 7.12 (d, 7=2.0 Hz, 1 H) 7.21 (dd, 7=8.7,1.9 Hz, 1 H) 7.38 (d, 7=8.4 Hz, 1 H) 7.57 (d, 7=8.8 Hz, 1 H) 8.07 (d, 7=0.9 Hz, 1 H) 8.77 (s, 1 H)

12.38 (brs, 1 H)

LC/MS (method LC-A): R_t 1.15 min, MH* 613

[a]_D ²⁰: +141.7° (c 0.525, DMF)

Chiral SFC (method SFC-C): R_t 2.92 min, MH* 613, chiral purity 100%.

Example 11 : synthesis of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-2-((3methoxy-5-(methylsulfonyl)phenyl)amino)-1-(6-methoxy-5-(trifluoromethoxy)20 1/-/-indol-3-yl)ethanone (Compound 11) and chiral séparation to afford the Enantiomers 11A and 11B

f₃co

MeO

EtOH, NaOEt, -15°C 2h, rt12h

11c CH₂CI₂, 0°C 3h 11e

Chiral séparai

Enantiomers 11A and 11B

Synthesis of intermediate 11a:

To a cooled (-15°C) solution of 3-methoxy-4-(trifluoromethoxy)benzaldehyde

[CAS 853771-90-1] (50 g, 230 mmol) and ethyl azidoacetate (89 g, 690 mmol) in EtOH (400 mL) was added dropwise, over a period of 2 h, a solution of NaOEt (0.69 mol, prepared from 15.9 g Na and 700 mL of EtOH). The reaction mixture was stirred at room température overnight. After cooling on an ice-bath, the reaction was quenched with a saturated NH4CI solution (1.2 L), and stirred for 10 min. The precipitate was filtered off, washed with water, and dried to give (Z)-ethyl 2-azido-3-(3-methoxy-4-(trifluoromethoxy)phenyl)acrylate 11a (32 g) as a yellowish solid.

Synthesis of intermediate 11b:

A solution of (Z)-ethyl 2-azido-3-(3-methoxy-4-(trifluoromethoxy)phenyl)acrylate 11a (3 g, 10 mmol) in xylene (40 mL) was heated under reflux overnight. After cooling to room température, the solvent was evaporated to dryness. The residue was triturated with hexane (50 mL) and the precipitate was filtered off

-50to afford methyl 6-methoxy-5-(trifluoromethoxy)-1H-indole-2-carboxylate 11b (yield: 1.4-1.6 g) as a yellow solid.

Synthesis of intermediate 11c:

To a mixture of methyl 6-methoxy-5-(trifluoromethoxy)-1H-indole-2-carboxylate 11b (25 g, 87 mmol) in MeOH/H₂O (2/1,300 mL) was added NaOH (7 g, 175 mmol) and the mixture was heated under reflux until a clear solution was obtained. After cooling to room température, most of the methanol was removed under reduced pressure and the remaining aqueous solution was acidified with conc. HCl to pH 3-4. The product was extracted with EtOAc (2x 250 mL). The combined organic layers were washed with brine, dried, and evaporated under reduced pressure to give 6-methoxy-5-(trifluoromethoxy)-1Hindole-2-carboxylic acid 11c (22.7 g) as a grey solid.

Synthesis of intermediate 11 d:

A suspension of 6-methoxy-5-(trifluoromethoxy)-1/-/-indole-2-carboxylic acid 11c (7.5 g, 27 mmol) and Cu (1.22 g, 0.7 equiv.) in quinoline (150 mL) was heated to 220-230°C under inert atmosphère for 12 h. After cooling to room température, the mixture was diluted with methyl tert-butyl ether (MTBE, 400 mL) and washed with a saturated aqueous NaHSO₄ solution (2x 500 mL). The organic layer was dried over MgSO₄, filtered through short pad of silica gel, and evaporated under reduced pressure. The residue was purified by column chromatography to afford 6-methoxy-5-(trifluoromethoxy)-1/-/-indole 11d (3.75 g) as a yellow solid.

Synthesis of the intermediate 11e:

Diethylaluminum chloride 1M in hexane (9.7 mL, 9.7 mmol) was added dropwise at 0°C to a solution of 6-methoxy-5-(trifluoromethoxy)-1 H-indole 11d (1.5 g, 6.5 mmol) in CH₂CI₂ (25 mL). After 30 min at 0°C, 2-(2-(2(benzyloxy)ethoxy)-4-chlorophenyl)acetyl chloride 1e (2.4 g, 7.13 mmol) in CH₂CI₂ (25 mL) was added dropwise. The reaction was stirred at 0°C for 3 h. The reaction was carefully quenched at 0°C with ice. Water was added, the layers were separated and the aqueous layer was extracted with CH₂CI₂. The combined organic layers were dried over MgSO₄, filtered, and solvent was evaporated under reduced pressure. Purification was carried out by flash chromatography on silica gel (15-40 pm, 120 g, heptane/EtOAc 80/20). The pure fractions were combined and evaporated to dryness to afford 2-(2-(218639

-51(benzyloxy)ethoxy)-4-chlorophenyl)-1-(6-methoxy-5-(trifluoromethoxy)-1/7indol-3-yl)ethanone 11e (2.1 g).

Synthesis of the intermediate 11g:

Under a N2-flow, at 0°C, a solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (1.06 g, 2.81 mmol) in THF (37 mL) was added dropwise to a solution of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1 -(6-methoxy-5(trifluoromethoxy)-1/7-indol-3-yl)ethanone 11e (1.5 g, 2.81 mmol) in THF (38 mL). The mixture was stirred at 0°C for 1 h. The cooling bath was removed îo and stirring was continued at room température for 3 h. A solution of 3methoxy-5-(methylsulfonyl)aniline [CAS 62606-02-4] (1.69 g, 8.43 mmol) in CH3CN (30 mL) was added and the resulting mixture was stirred at 50°C for 48 h. The mixture was concentrated under reduced pressure. The residue was taken up with EtOAc, washed with water, 1N HCl (3 times), and then with a solution of 10% K2CO3 in water. The organic layer was dried over MgSO₄, filtered, and the solvent was concentrated under reduced pressure. Purification was performed by flash chromatography on silica gel (15-40 pm, 80 g, eluent: CH2CI2/CH3OH 99.5/0.5). The pure fractions were combined and evaporated to dryness. The residue was purified again by flash chromatography on silica gel (15-40 pm, 40 g, eluent: heptane/EtOAc gradient 60/40 to 50/50). The pure fractions were combined and evaporated to dryness yielding 2-(2-(2(benzyloxy)ethoxy)-4-chlorophenyl)-2-((3-methoxy-5(methylsulfonyl)phenyl)amino)-1-(6-methoxy-5-(trifluoromethoxy)-1/7-indol-3yl)ethanone 11g (1.075 g).

Synthesis of Compound 11 and chiral séparation into Enantiomers 11A and 11B:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-2-((3-methoxy-5(methylsulfonyl)phenyl)amino)-1-(6-methoxy-5-(trifluoromethoxy)-1/7-indol-330 yl)ethanone 11g (934 mg, 1.27 mmol) in CH₃OH (18 mL) was hydrogenated for 1 h under atmospheric pressure of H₂ using 10% Pd/C (271 mg, 0.255 mmol) as the catalyst. The reaction was diluted with CH2CI2 and filtered through a pad of celite®. The filtrate was evaporated under reduced pressure. Purification was carried out by flash chromatography on silica gel (15-40 pm, 24 g, eluent:

CH2CI2/CH3OH:99/1 ). The pure fractions were combined and evaporated to dryness to afford 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-2-((3-methoxy-5(methylsulfonyl)phenyl)amino)-1-(6-methoxy-5-(trifluoromethoxy)-1/7-indol-3yl)ethanone (compound 11, 540 mg) as a racemic mixture. The enantiomers of

-52Compound 11 (540 mg) were separated via chiral SFC (Stationary phase: Chiralpak® IA 5 pm 250 x 20 mm, Mobile phase: 70% CO₂, 30% iPOH + 0.3% iPrNH₂) yielding 250 mg of the first eluted enantiomer and 260 mg of the second eluted enantiomer. The first eluted enantiomer was precipitated from diisopropyl ether/Et₂O/heptane. The precipitate was filtered off and dried to give 209 mg of Enantiomer 11 A. The second eluted enantiomer was precipitated from diisopropyl ether. The precipitate was filtered off and dried to give 172 mg of Enantiomer 11 B.

Compound 11:

¹H NMR (500 MHz, DMSO-d₆) δ ppm 3.08 (s, 3 H) 3.72 (s, 3 H) 3.84 - 4.04 (m, 5 H) 4.15 - 4.23 (m, 2 H) 5.28 (br s, 1 H) 6.36 (d, 7=7.9 Hz, 1 H) 6.58 (t, 7=1.9 Hz, 1 H) 6.64 (s, 1 H) 6.93 (s, 1 H) 6.96 (dd, 7=8.2,1.9 Hz, 1 H) 7.06 (d, 7=7.9 Hz, 1 H) 7.12 (d, 7=1.9 Hz, 1 H) 7.17 (s, 1 H) 7.37 (d, 7=8.5 Hz, 1 H) 8.03 (d, 7=1.3 Hz, 1 H) 8.63 (s, 1 H) 12.16 (br s, 1 H) LC/MS (method LC-C): R_t 3.10 min, MH* 643

Melting point: 212°C

Enantiomer 11 A:

¹H NMR (500 MHz, DMSO-d₆) δ ppm 3.08 (s, 3 H) 3.72 (s, 3 H) 3.87 (s, 3 H) 3.89 - 4.05 (m, 2 H) 4.19 (br t, 7=4.4 Hz, 2 H) 5.30 (br s, 1 H) 6.36 (d, 7=7.6 Hz, 1 H) 6.58 (t, 7=1.6 Hz, 1 H) 6.64 (br s, 1 H) 6.93 (s, 1 H) 6.96 (dd, 7=8.4,1.7 Hz, 1 H) 7.07 (d, 7=7.9 Hz, 1 H) 7.12 (d, 7=1.9 Hz, 1 H) 7.17 (s, 1 H) 7.37 (d, 7=8.5 Hz, 1 H) 8.04 (d, 7=0.6 Hz, 1 H) 8.63 (s, 1 H) 12.10 (br s, 1 H) LC/MS (method LC-C): R_t 3.09 min, MH* 643 [a]_D ²⁰: -102.3° (c 0.208, DMF)

Chiral SFC (method SFC-F): R_t 3.61 min, M-F 625, chiral purity 100%.

Enantiomer 11 B:

¹H NMR (500 MHz, DMSO-7₆) δ ppm 3.09 (s, 3 H) 3.72 (s, 3 H) 3.87 (s, 3 H) 3.88 - 4.04 (m, 2 H) 4.19 (t, 7=4.6 Hz, 2 H) 5.30 (t, 7=5.5 Hz, 1 H) 6.36 (d, 7=7.9 Hz, 1 H) 6.58 (t, 7=1.7 Hz, 1 H) 6.64 (br s, 1 H) 6.93 (s, 1 H) 6.96 (dd, 7=8.2, 1.9 Hz, 1 H) 7.07 (d, 7=7.9 Hz, 1 H) 7.12 (d, 7=1.9 Hz, 1 H) 7.18 (s, 1 H) 7.37 (d, 7=8.2 Hz, 1 H) 8.04 (d, 7=0.9 Hz, 1 H) 8.63 (s, 1 H) 12.10 (brs, 1 H)

LC/MS (method LC-C): R_t 3.09 min, MH* 643 [a]_D ²⁰: +101.8° (c 0.208, DMF)

-53Chiral SFC (method SFC-F): R_t 4.38 min, MH⁺ 643, chiral purity 98.7%.

Example 12: synthesis of 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-2-((3methoxy-5-(methylsulfonyl)phenyl)amino)-1-(7-methyl-5-(trifluoromethoxy )-1/-/5 indol-3-yl)ethanone (Compound 12) and chiral séparation into Enantiomers 12Aand 12B.

NaBH₄ fBuOH, H₂O 90°C, 2.5h

AlCh. BCI₃CH₂CI₂0°C to reflux 8h

Enantiomers 12A and

Synthesis of intermediate 12a:

A mixture of boron(lll) chloride 1M in CH2CI2 (25.5 mL, 25.5 mmol) and aluminum(lll) chloride (3.40 g, 25.5 mmol) was diluted with CH2CI2 (20 mL) and cooled on an ice-bath under N₂-atmosphere. A solution of 2-methyl-415 (trifluoromethoxy)aniline [CAS 86256-59-9] (4.88 g, 25.5 mmol) and chloroacetonitrile (3.24 mL, 51.0 mmol) in CH2CI2 (7.5 mL) was added dropwise. After addition, the ice-bath was removed and the mixture was heated under reflux for 8 h. The mixture was cooled again to 0°C using an ice-bath. 2N HCl (75 mL) was added dropwise, causing heavy précipitation. The resulting suspension was heated under reflux for 90 min, and cooled to room

-54temperature. The solids were removed by filtration. The filter cake was washed with CH2CI2 (4x). The filtrâtes were combined and the phases were separated. The organic layer was isolated, washed with an aqueous NaHCOs solution, dried over MgSO4, filtered and evaporated under reduced pressure. The residue was purified by flash chromatography (Stationary phase: Biotage® SNAP Ultra Silica 100 g, Mobile phase: heptane/CH2Cl2 gradient 100/0 to 0/100). The desired fractions were combined and concentrated to a residual volume of 30 mL. The precipitate was filtered off, washed with heptane and CH2CI2, and dried under vacuum at 50°C to provide 1 -(2-amino-3-methyl-510 (trifluoromethoxy)phenyl)-2-chloroethanone 12a (1.37 g). The filtrate was concentrated under reduced pressure. The solid residue was stirred up in a mixture of heptane (20 mL) and diisopropyl ether (3 mL), filtered off, washed with heptane (3x) and dried under vacuum at 50°C to provide a second fraction of12a (0.24 g).

Synthesis of intermediate 12b:

Sodium borohydride (326 mg, 8.61 mmol) was added to a stirred solution of 1-(2-amino-3-methyl-5-(trifluoromethoxy)phenyl)-2-chloroethanone 12a (1.92 g, 7.17 mmol) in tert-butanol (50 mL) and water (5 mL). The reaction mixture was stirred at room température for 30 min and at 90°C for 2.5 h. Water (50 mL) was added and the product was extracted with diethyl ether (2x). The combined organic layers were washed with brine, dried over MgSÛ4, filtered and evaporated under reduced pressure. The residue was purified by flash chromatography (Stationary phase: Biotage® SNAP Ultra silica 25 g, Mobile phase: heptane/EtOAc gradient 100/0 to 20/80). The desired fractions were combined, concentrated under reduced pressure, co-evaporated with heptane and dried under vacuum at 50°C to provide 7-methyl-5-(trifluoromethoxy)-1 Hindole 12b (1.2 g).

Synthesis of intermediate 12c:

Diethylaluminum chloride 1M in hexane (18.2 mL, 18.2 mmol) was added dropwise, at 0°C and under N2-atmosphere, to a solution of 7-methyl-5(trifluoromethoxy)-l/-/-indole 12b (2.0 g, 9.3 mmol) in CH2CI2 (150 mL). After stirring for 25 min at 0°C, a solution of 2-(2-(2-(benzyloxy)ethoxy)-435 chlorophenyl)acetyl chloride 1e (4.72 g, 13.9 mmol, synthesis: see Example 1 ) in CH2CI2 (75 mL) was slowly added, keeping the reaction température below 5°C. The reaction mixture was stirred at 0°C for 90 min and at room température for 2 h. The reaction mixture was cooled to 0°C and a solution of

-55Rochelle sait [CAS 6100-16-9] (5.25 g, 18.6 mmol) in water (5.5 mL) was added dropwise. The mixture was vigorously stirred for 30 min at 0°C. The ice-bath was removed and THF (200 mL) was added. After stirring for 1 h at room température, Na2SO₄ (25 g) was added. The mixture was stirred for 18 h and filtered over dicalite®. The filter cake was washed with THF (4x 150 mL) and the combined filtrâtes were evaporated under reduced pressure. The remaining oil was purified by flash chromatography (Stationary phase: Biotage® SNAP Ultra silica 100 g, Mobile phase: heptane/EtOAc/EtOH gradient 100/0/0 to 40/45/15). The desired fractions were combined and ίο evaporated under reduced pressure. The solid residue was stirred up in a mixture of DIPE (25 mL) and EtOAc (2 mL), filtered off, washed with DIPE (3x), and dried at 50°C under vacuum to give 2-(2-(2-(benzyloxy)ethoxy)-4chlorophenyl)-1-(7-methyl-5-(trifluoromethoxy)-1H-indol-3-yl)ethanone 12c (2.88 g).

Synthesis of intermediate 12d:

A mixture of 2-(2-(2-(benzyloxy)ethoxy)-4-chlorophenyl)-1-(7-methyl-5-(trifluoromethoxy)-1/-/-indol-3-yl)ethanone 12c (2.88 g, 5.56 mmol) and 10% palladium on carbon (0.5 g) in EtOAc (75 mL) and THF (10 mL) was stirred at room température for 20 min under H2 atmosphère. The reaction mixture was filtered through dicalite® and the filter cake was washed with THF. The combined filtrâtes were concentrated under reduced pressure. The residue was purified by flash chromatography (stationary phase: Biotage® SNAP Ultra silica 50 g, Mobile phase: heptane/EtOAc/EtOH gradient 100/0/0 to 40/45/15). The fractions containing product were combined and evaporated under reduced pressure. The solid residue was stirred in DIPE (7.5 mL), filtered off, washed with DIPE (2x), and dried under vacuum at 45°C to provide 2-(4-chloro-2-(2hydroxyethoxy)phenyl)-1-(7-methyl-5-(trifluoromethoxy)-1/7-indol-3-yl)ethanone 12d (780 mg).

Synthesis of intermediate 12e:

Phenyltrimethylammonium tribromide [CAS 4207-56-1] (327 mg, 0.869 mmol) was added to a cooled (0°C) solution of 2-(4-chloro-2-(2hydroxyethoxy)phenyl)-1-(7-methyl-5-(trifluoromethoxy)-1/7-indol-3-yl)ethanone

12d (354 mg, 0.827 mmol) in THF (15 mL), under N2-atmosphere. The reaction mixture was stirred at 0°C for 45 min and at room température for 75 min. The precipitate was filtered off and washed with THF (2x). The combined filtrâtes were concentrated under reduced pressure to give 2-bromo-2-(4-chloro-2-(2

-56hydroxyethoxy)phenyl)-1-(7-methyl-5-(trifluoromethoxy)-1/-/-indol-3-yl)ethanone 12e (419 mg) which was used in the next step without further purification.

Synthesis of Compound 12 and chiral séparation into Enantiomers 12A 5 and 12B:

A mixture of 2-bromo-2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-1-(7-methyl5-(trifluoromethoxy)-1/-/-indol-3-yl)ethanone 12e (419 mg, 0.827 mmol), 3-methoxy-5-(methylsulfonyl)aniline [CAS 62606-02-4] (333 mg, 1.65 mmol) and diisopropylethylamine (285 pL, 1.65 mmol) in CH3CN (30 mL) was stirred 10 at 60°C for 20 h The reaction was continued at 70°C for 7 h, and at room température for 65 h. The volatiles were evaporated under reduced pressure. The solid residue was combined with another fraction (1.14 g) and purified by flash chromatography (stationary phase: Grâce Reveleris® silica 40 g, Mobile phase: heptane/EtOAc/EtOH gradient 100/0/0 to 40/45/15). The desired fractions were combined and evaporated under reduced pressure. The residue was purified via préparative HPLC (Stationary phase: RP XBridge® Prep C18 OBD -10 pm, 5 Ox 150 mm, Mobile phase: 0.25% NH4HCO3 solution in water, CH3CN). The desired fractions were combined and evaporated under reduced pressure. The product was crystallized at room température from EtOH (10 mL), 20 filtered off, washed with EtOH (2x), and dried at 45°C under vacuum to provide racemic 2-(4-chloro-2-(2-hydroxyethoxy)phenyl)-2-((3-methoxy-5(methylsulfonyl)phenyl)amino)-1-(7-methyl-5-(trifluoromethoxy)-1/-/-indol-3yl)ethanone (Compound 12, two crops: 485 mg and 169 mg).

The chiral séparation of the enantiomers of Compound 12 (602 mg) was 25 performed via Normal Phase Chiral séparation (Stationary phase: Whelk01 (R,R), Mobile phase: 30% éthanol, 70% heptane). The product fractions were combined and evaporated to provide Enantiomer 12A as the first eluted product and Enantiomer 12B as the second eluted product. Both enantiomers were stirred up in a mixture of water (3.5 mL) and MeOH (1.25 mL), filtered off, 30 washed with water/MeOH 3/1 (4x) and dried under vacuum at 45°C to provide

Enantiomer 12A (202 mg) and Enantiomer 12B (166 mg).

Compound 12:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 2.51 (s, 3 H) 3.08 (s, 3 H) 3.72 (s, 3 H) 35 3.90 - 4.06 (m, 2 H) 4.19 (t, >4.6 Hz, 2 H) 5.28 (t, >5.7 Hz, 1 H) 6.41 (d, >7.7 Hz, 1 H) 6.58 (t, >1.8 Hz, 1 H) 6.66 (t, >2.1 Hz, 1 H) 6.92 - 6.98 (m, 2 H) 7.02 - 7.08 (m, 2 H) 7.11 (d, >2.0 Hz, 1 H) 7.37 (d, >8.4 Hz, 1 H) 7.92 (br s, 1 H) 8.70 (s, 1 H) 12.38 (s, 1 H)

-57LC/MS (method LC-A): R_t 1.18 min, MH* 627

Enantiomer 12A:

¹H NMR (400 MHz, DMSO-d₆) δ ppm 2.51 (br s, 3 H) 3.09 (s, 3 H) 3.72 (s, 3 H) 5 3.88 - 4.09 (m, 2 H) 4.19 (br t, 7=4.5 Hz, 2 H) 5.28 (br t, 7=5.4 Hz, 1 H) 6.41 (br d, J=7.7 Hz, 1 H) 6.58 (br s, 1 H) 6.66 (br s, 1 H) 6.91 - 6.99 (m, 2 H)

7.01 - 7.08 (m, 2 H) 7.11 (br s, 1 H) 7.37 (d, 7=8.4 Hz, 1 H) 7.91 (br s, 1 H)

8.70 (s, 1 H) 12.38 (brs, 1 H)

LC/MS (method LC-A): R_t 1.21 min, MH* 627

[a]_D ²⁰: -111.0° (c 0.51, DMF)

Chiral SFC (method SFC-C): R_t 3.31 min, MH* 627, chiral purity 100%.

Enantiomer 12B:

Ή NMR (400 MHz, DMSO-d₆) δ ppm 2.51 (br s, 3 H) 3.09 (s, 3 H) 3.72 (s, 3 H) 15 3.90 - 4.06 (m, 2 H) 4.19 (t, 7=4.6 Hz, 2 H) 5.28 (br s, 1 H) 6.41 (d, 7=7.9 Hz,

H) 6.58 (t, 7=1.8 Hz, 1 H) 6.66 (br t, 7=2.4 Hz, 1 H) 6.92 - 6.99 (m, 2 H)

7.01 - 7.08 (m, 2 H) 7.11 (d, 7=1.8 Hz, 1 H) 7.37 (d, 7=8.4 Hz, 1 H) 7.92 (br s,

H) 8.70 (s, 1 H) 12.38 (brs, 1 H)

LC/MS (method LC-A): R_t 1.21 min, MH* 627

[a]_D ²⁰: +105.2° (c 0.515, DMF)

Chiral SFC (method SFC-C): R_t 2.91 min, MH* 627, chiral purity 98.5%.

ANTIVIRAL ACTIVITY OF THE COMPOUNDS OF THE INVENTION

DENV-2 antiviral assay

The antiviral activity of ail the compounds of the invention was tested against the DENV-2 16681 strain which was labeled with enhanced green fluorescent protein (eGPF). The culture medium consists of minimal essential medium supplemented with 2% of heat-inactivated fêtai calf sérum, 0.04% gentamycin (50mg/mL) and 2mM of L-glutamine. Vero cells, obtained from ECACC, were suspended in culture medium and 25pL was added to 384-well plates (2500 cells/well), which already contain the antiviral compounds. Typically, these plates contain a 5-fold serial dilution of 9 dilution steps of the test compound at 200 times the final concentration in 100% DMSO (200nL). In addition, each compound concentration is tested in quadruplicate (final concentration range: 25μΜ - 0.000064μΜ or 2.5μΜ - 0.0000064μΜ for the most active compounds). Finally, each plate contains wells which are assigned as virus Controls (containing cells and virus in the absence of compound), cell

-58controls (containing cells in the absence of virus and compound) and medium Controls (containing medium in the absence of cells, virus and compounds). To the wells assigned as medium control, 25pL of culture medium was added instead of Vero cells. Once the cells were added to the plates, the plates were 5 incubated for 30 minutes at room température to allow the cells to distribute evenly within the wells. Next, the plates were incubated in a fully humidified incubator (37°C, 5%CO₂) until the next day. Then, DENV-2 strain 16681, labeled with eGFP, was added at a multiplicity of infection (MOI) of 0.5.

Therefore, 15 pL of virus suspension was added to ail the wells containing test ίο compound and to the wells assigned as virus control. In parallel, 15pL of culture medium was added to the medium and cell Controls. Next, the plates were incubated for 3 days in a fully humidified incubator (37°C, 5% CO₂). At the day of the read out, the eGFP fluorescence was measured using an automated fluorescence microscope at 488 nm (blue laser). Using an in-house LIMS

System, inhibition dose response curves for each compound were calculated and the half maximal effective concentration (EC₅₀) was determined. Therefore, the percent inhibition (I) for every test concentration is calculated using the following formula: I = 1 00*(St-Scc)/(Svc-Scc); St, Sec and S_Vc are the amount of eGFP signal in the test compound, cell control and virus control wells, respectively. The ECso represents the concentration of a compound at which the virus réplication is inhibited with 50%, as measured by a 50% réduction of the eGFP fluorescent intensity compared to the virus control. The ECso is calculated using linear interpolation (Table 1).

In parallel, the toxicity of the compounds was assessed on the same plates.

Once the read-out for the eGFP signal was done, 40pL of ATPIite, a cell viability stain, was added to ail wells of the 384-well plates. ATP is présent in ail metabolically active cells and the concentration déclinés very rapidly when the cells undergo necrosis or apoptosis. The ATPLite assay System is based on the production of light caused by the reaction of ATP with added luciferase and

D-luciferin. The plates were incubated for 10 minutes at room température.

Next, the plates were measured on a ViewLux. The half maximal cytotoxic concentration (CC50) was also determined, defined as the concentration required to reduce the luminescent signal by 50% compared to that of the cell control wells. Finally, the selectivity index (SI) was determined for the compounds, which was calculated as followed: SI = CC50/EC50·

In parallel, the toxicity of the compounds was assessed on the same plates. Once the read-out for the eGFP signal was done, 40pL of ATPIite, a cell viability stain, was added to ail wells of the 384-well plates. ATP is présent in ail

-59metabolically active cells and the concentration déclinés very rapidly when the cells undergo necrosis or apoptosis. The ATPLite assay System is based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin. The plates were incubated for 10 minutes at room température.

Next, the plates were measured on a ViewLux. The half maximal cytotoxic concentration (CC50) was also determined, defined as the concentration required to reduce the luminescent signal by 50% compared to that of the cell control wells. Finally, the selectivity index (SI) was determined for the compounds, which was calculated as followed: SI = CC50/EC50.

.0

Table 1: ECsn, CC50, and SI for the compounds of the invention in the DENV-2 antiviral assay

compound#	ECso (μΜ)	N	CC50 (μΜ)	N	SI	N
1	0.00043	4	5.1	4	11800	4
1A	0.00024	7	3.7	7	>13500	7
1B	0.0035	4	8.4	4	2420	4
2	0.00027	4	4.2	4	>10300	4
2A	0.00011	10	3.4	10	>37500	9
2B	0.0049	4	11	4	2130	4
3	0.00054	5	12	5	>31100	5
3A	0.00032	6	5.2	6	16400	6
3B	0.056	4	13	4	226	4
4	0.00042	4	3.7	4	>7400	4
4A	0.00013	9	3.0	9	>23200	9
4B	0.011	5	5.7	6	509	5
5	0.00010	11	4.3	12	>35600	11
5A	0.0084	4	5.0	4	595	4
5B	0.000066	5	3.4	5	>43900	5
6	0.00037	4	4.7	4	12700	4
6A	0.00013	5	3.8	6	>38500	5
6B	0.036	5	5.9	4	164	4
7	0.00026	5	2.8	6	>10500	5
7A	0.00022	5	2.7	4	12573	3
7B	0.0050	3	9.2	3	1850	3
8	0.00027	3	2.8	3	>9820	3
8A	0.00011	4	>2.5	4	25900	4

compound#	EC50 (μΜ)	N	CCso (μΜ)	N	SI	N
8B	0.0017	4	>2.4	5	>1450	4
9	0.00010	4	>2.5	4	>30100	4
9A	0.0019	4	11	4	5720	4
9B	0.000061	4	>2.4	4	>39700	4
10	0.00010	4	>2.4	4	>29600	4
10A	0.0029	3	2.3	3	783	3
10B	0.000067	5	2.4	5	>42700	5
11	0.00020	4	>2.5	4	17200	4
11A	0.0015	4	>2.3	4	>2220	4
11B	0.000089	3	>2.3	3	>26400	3
12	0.00010	6	2.5	6	>45600	6
12A	0.0050	6	2.5	7	1030	6
12B	0.000034	9	2.5	11	>390600	9

Tetravalent reverse transcriptase quantitative-PCR (RT-qPCR) assay: Protocol A.

The antiviral activity of the compounds of the invention was tested against

DENV-1 strain TC974#666 (NCPV), DENV-2 strain 16681, DENV-3 strain H87 (NCPV) and DENV-4 strains H241 (NCPV) and EDEN (SG/06K2270DK1/2005; GenBank accession number QG398256) in a RT-qPCR assay. Therefore, Vero cells were infected with either DENV-1, or -2, or -3, or -4 in the presence or absence of test compounds. At day 3 post-infection, the cells were lysed and cell lysâtes were used to préparé cDNA of both a viral target (the 3’UTR of DENV; Table 2) and a cellular reference gene (β-actin, Table 2). Subsequently, a duplex real time PCR was performed on a Lightcycler480 instrument. The generated Cp value is inversely proportional to the amount of RNA expression of these targets. Inhibition of DENV réplication by a test compound results in a shift of Cp’s for the 3’UTR gene. On the other hand, if a test compound is toxic to the cells, a similar effect on β-actin expression will be observed. The comparative AACp method is used to calculate EC₅o, which is based on the relative gene expression of the target gene (3’UTR) nonmalized with the cellular housekeeping gene (β-actin). In addition, CC50 values are determined based on the Cp values acquired for the housekeeping gene β-actin.

-61Table 2: Primers and probes used for the real-time, quantitative RT-PCR.

Primer/probe	Target	Sequence^a*^b
F3utr258	DENV 3’-UTR	5'-CGGTTAGAGGAGACCCCTC-3'
R3utr425	DENV 3’-UTR	5'-GAGACAGCAGGATCTCTGGTC-3'
P3utr343	DENV 3'-UTR	FA/W-5'-AAG G ACTAG-ZE Ν- ΑΟ GTTAG AG G AG AC CCCCC-3'-/A BkFQ
Factin743	β-actin	5'-GGCCAGGTCATCACCATT-3'
Ractin876	β-actin	5'-ATGTCCACGTCACACTTCATG-3'
Pactin773	β-actin	HEX-5'-TTCCGCTGC-ZEA/-CCTGAGGCTCTC-3'lABkFQ

^a Reporter dyes (FAM, HEX) and quenchers (ZEN and lABkFQ) éléments are indicated in bold and italics.

^b The nucléotide sequence of the primers and probes were selected from the conserved région in the 3'UTR région of the dengue virus genome, based on the alignment of 300 nucléotide sequences of the four dengue serotypes deposited in Genbank (Gong et al., 2013, Methods Mol Biol, Chapter 16).

The culture medium consisted of minimal essential medium supplemented with 2% of heat-inactivated fêtai calf sérum, 0.04% gentamycin (50mg/mL) and 2mM of L-glutamine. Vero cells, obtained from ECACC, were suspended in culture medium and 75pL/well was added in 96-well plates (10000 cells/well), which already contain the antiviral compounds. Typically, these plates contain a 5-fold serial dilution of 9 dilution steps of the test compound at 200 times the final concentration in 100% DMSO (500nL; final concentration range:

25μΜ - 0.000064pM or 2.5μΜ - 0.0000064μΜ for the most active compounds). In addition, each plate contains wells which are assigned as virus Controls (containing cells and virus in the absence of compound) and cell Controls (containing cells in the absence of virus and compound). Once the cells were added in the plates, the plates were incubated in a fully humidified incubator (37°C, 5%CO₂) until the next day. Dengue viruses serotype-1,2, 3 and 4 were diluted in order to obtain a Cp of -22-24 in the assay. Therefore, 25pL of virus suspension, was added to ail the wells containing test compound and to the wells assigned as virus control. In parallel, 25pL of culture medium was added to the cell Controls. Next, the plates were incubated for 3 days in a fully humidified incubator (37°C, 5%CO₂). After 3 days, the supernatant was removed from the wells and the cells were washed twice with ice-cold PBS (~100pL). The cell pellets within the 96-well plates were stored at -80°C for at

-62least 1 day. Next, RNA was extracted using the Cells-to-CT™ lysis kit, according to the manufacturer’s guideline (Life Technologies). The cell lysâtes can be stored at -80°C or immediately used in the reverse transcription step.

In préparation of the reverse transcription step, mix A (table 3A) was prepared and 7.57pL/well was dispensed in a 96-well plate. After addition of 5pL of the cell lysâtes, a five minute dénaturation step at 75°C was performed (table 3B). Afterwards, 7.43pL of mix B was added (table 3C) and the reverse transcription step was initiated (table 3D) to generate cDNA.

Finally, a RT-qPCR mix was prepared, mix C (table 4A), and 22.02 pL/well was dispensed in 96-well LightCycler qPCR plates to which 3pL of cDNA was added and the qPCR was performed according to the conditions in table 4B on a LightCycler 480.

Using the LightCycler software and an in-house LIMS System, dose response curves for each compound were calculated and the half maximal effective concentration (EC50) and the half maximal cytotoxic concentration (CC50) were determined (Tables 5-8).

Table 3: cDNA synthesis using Mix A, dénaturation, Mix B and reverse transcription.

Mix A

Plates	8
Samples	828		Reaction Vol. (pl)	20
Mix Item	Concentration	Volume for (μΙ)
Unit	Stock	Final	1 sample	x samples
Milli-Q H₂O				7.27	6019.56
R3utr425	μΜ	20	0.27	0.15	124.20
Ractin876	μΜ	20	0.27	0.15	124.20
	Volume mix/well (Ml)	7.57
Cell lysâtes	5.00

-63Denaturation

B step:

Step	Temp	Time
Dénaturation	75°C	5'
Hold	4°C	hold

C Mix B

Samples	864
Mix Item	Concentration	Volume for (μΓ
Unit	Stock Final	1 sample	x samples
Expand HIFI buffet 2	X	10.00	1.00	2.00	1728.0
MgCI₂	mM	25.00	3.50	2.80	2419.2
dNTPs	mM	10.00	1.00	2.00	1728.0
Rnase inhibitor	U/μΙ	40.00	1.00	0.50	432.0
Expand RT	U/μΙ	50.00	0.33	0.13	112.3
	Total Volume Mix (μΙ)	7.43

D Protocol cDNA synthesis

Step	Temp	Time
Rev transe	42°C	30'
Dénaturation	99°C	5'
Hold	4°C	hold

I

-64Table 4: qPCR mix and protocol.

A MixC

Samples	833		Reaction Vol. (Ml)	25
Mix Item	Concentration	Volume for (μΙ)
Unit	Stock	Final	1 sample	x samples
H₂0 PCR grade Roche				7.74	6447.42
Roche 2xMM mix	X	2	1	12.50	10412.50
F3utr258	μΜ	20	0.3	0.38	316.54
R3utr425	μΜ	20	0.3	0.38	316.54
P3utr343	μΜ	20	0.1	0.13	108.29
Factin743	μΜ	20	0.3	0.38	316.54
Ractin876	μΜ	20	0.3	0.38	316.54
Pactin773	μΜ	20	0.1	0.13	108.29
	Volume Mix / Tube (μΙ)	22.02
cDNA	3.00

B Protocol qPCR3

Step	Temp	Time	Ramp rate
preincub/denat	95°C	10 min	4.4
Dénaturation	95°C	10 sec	4.4	40 cycles
annealing	58°C	1 min	2.2
Elongation	72°C	1 sec	4.4
Cooling	40°C	10 sec	1.5

-65Table 5: ECso, CCso, and SI for the compounds aqainst serotype 1 in the RTqPCR assavs

	Protocol A
RI	Γ-qPCR serotype 1 TC974#666
	EC50		CC50
compound#	(μΜ)	N	(μΜ)	N	SI	N
1A	0.0013	3	>2.5	3	>2200	3
2A	0.0016	5	>2.5	5	>1980	5
3A	0.0042	4	5.8	4	1380	4
4A	0.0017	4	>2.5	4	>2160	4
5B	0.0016	3	>2.5	3	>967	3
6A	0.0016	6	4.9	5	2830	5
7A	0.00097	3	2.9	2	5380	2
8A	0.00039	3	6.5	3	17200	3
9B	0.000083	6	2.9	6	>38700	6
10B	0.000071	6	>2.4	7	>41300	6
11B	0.00012	3	>2.5	3	>26300	3
12B	0.000086	5	>2.3	5	>26700	5

N= the number of independent experiments in which the compounds were tested.

Table 6: ECso, CC50, and SI for the compounds aqainst serotype 2 in the RTqPCR assavs

	Protocol A
RT-qPCR serotype 2 16681__________
	EC50		CC50
compound#	(μΜ)	N	(μΜ)	N	SI	N
1A	0.00024	4	4.1	4	29700	4
2A	0.00014	7	4.0	9	>29700	7
3A	0.00040	5	5.3	5	16800	5
4A	0.00016	7	3.7	8	40100	7
5B	0.000065	5	3.6	6	>52300	5
6A	0.00021	5	4.5	8	65400	5
7A	0.00021	3	3.8	4	>14800	3
8A	0.00027	3	6.1	4	89000	3
9B	0.000054	3	>2.5	4	>49900	3

	Protocol A
RT-qPCR serotype 2 16681
compound#	EC50 (pM)	N	CC50 (μΜ)	N	SI	N
10B	0.000049	3	>2.5	3	>62800	3
11B	0.000062	5	2.9	4	>46200	4
12B	0.000053	3	>2.5	4	>56600	3

N= the number of independent experiments in which the compounds were tested.

Table 7: ECso, CCm, and SI for the compounds aqainst serotype 3 in the RTgPCR assavs

	Protocol A
RT-qPCR serotype 3	H87
	EC50		CC50
compound#	(μΜ)	N	(μΜ)	N	SI	N
1A	0.0096	3	>2.5	3	>421	3
2A	0.018	5	>2.5	5	>186	5
3A	0.042	4	4.0	4	110	4
4A	0.018	4	>2.5	4	>173	4
5B	0.012	4	>2.5	3	>218	3
6A	0.017	6	3.9	4	198	4
7A	0.011	3	>2.5	3	299	3
8A	0.0042	3	4.0	3	1020	3
9B	0.0012	3	>2.2	3	1970	3
10B	0.0014	3	>2.5	3	>2220	3
11B	0.0013	3	>2.5	3	>2210	3
12B	0.0011	3	>2.2	3	2720	3

N= the number of independent experiments in which the compounds were tested.

-67Table 8: EOn, CC50, and SI for the compounds against serotype 4 in the RTgPCR assavs.

	Protocol A
	RT-q	PCR sera	type 4	-1241
	EC50		CC50
compound#	(μΜ)	N	(μΜ)	N	SI	N
1A	0.071	4	>2.4	3	>29	3
2A	0.074	7	2.5	6	39	6
3A	0.11	5	3.0	3	21	3
4A	0.078	8	>2.2	6	24	6
5B	0.074	6	>2.2	6	33	6
6A	0.053	7	>2.5	7	58	7
7A	0.064	4	>2.3	3	37	3
8A	0.036	4	2.6	4	93	4
9B	0.0081	3	>2.5	3	>409	3
10B	0.0069	4	>2.2	4	>331	4
11B	0.011	4	2.2	3	202	3
12B	0.0055	3	>2.1	3	514	3

	Protocol A
	RT-qPCR serotype 4 EDEN
compound#	EC50 (μΜ)	N	CC50 (μΜ)	N	SI	N
1A	ND		ND		ND
2A	0.0013	5	> 2.5	5	> 2406	5
3A	0.0031	4	4.8	4	2088	4
4A	0.0014	4	> 2.5	4	> 2455	4
5B	0.0011	3	> 2.5	3	> 2989	3
6A	0.0010	5	> 2.5	4	> 2825	4
7A	0.00064	1	3.2	1	4953	1

N= the number of independent experiments in which the compounds were tested.

Claims

Claims

1. A compound of formula (I)

5 a stereo-isomeric form, a pharmaceutically acceptable sait, solvaté or polymorph thereof comprising a mono- or di-substituted indole group;

said compound is selected from the group wherein:

Ri is H, R₂ is F, Cl or OCH₃ and R₃ is H;

Ri is H, R₂ is F or Cl and R₃ is CH₃;

10 Ri is CH₃, R₂ is OCH₃ and R₃ is H;

Ri is F, R₂ is F and R₃ is H;

Ri is CH₃, R₂ is F and R₃ is H;

Ri is CF₃ or OCF₃ and R₂ is H and R₃ is H;

Ri is OCF₃, R₂ is OCH₃ and R₃ is H or

15 Ri is OCF₃, R₂ is H and R₃ is CH₃.
2. A compound or its stereo-isomeric form, a pharmaceutically acceptable sait, solvaté or polymorph thereof according to claim 1 wherein said compound is selected from the group:
3. A pharmaceutical composition comprising a compound of formula (I) or a stéréo- isomeric form , a pharmaceutically acceptable sait, solvaté or polymorph thereof according to claim 1 or 2 together with one or more pharmaceutically acceptable excipients, diluents or carriers.
4. A compound of formula (I) or a stéréo- isomeric form , a pharmaceutically acceptable sait, solvaté or polymorph thereof according to claim 1 or a pharmaceutical composition according to claim 3 for use as a médicament.
5. A compound of formula (I) or a stéréo- isomeric form, a pharmaceutically acceptable sait, solvaté or polymorph thereof according to claim 1 or a pharmaceutical composition according to claim 3 for use in the treatment

-70of dengue.
6. A use of a compound represented by the following structural formula (I) a stereo-isomeric form, a pharmaceutically acceptable sait, solvaté or polymorph thereof comprising a mono- or di-substituted indole group; said compound is selected from the group wherein:

Ri is H, R2 is F, Cl or OCH3 and R3 is H;

Ri is H, R2 is F or Cl and R3 is CH3;

Ri is CH₃, R2 is OCH3 and R3 is H;

Ri is F, R2 is F and R3 is H;

Ri is CH3, R2 is F and R3 is H;

Ri is CF3 or OCF3 and R2 is H and R3 is H;

Ri is OCF3, R₂ is OCH3 and R₃ is H or

Ri is OCF3 , R₂ is H and R3 is CH₃ for inhibiting the réplication of dengue virus(es) in a biological sample.
7. The use of a compound according to claim 6 which includes use of an additional therapeutic agent.
8. The use of claim 7 wherein said additional therapeutic agent is another antiviral agent.
9.

A use of a compound represented by the following structural formula (I)

-71 a stereo-isomeric form, a pharmaceutically acceptable sait, solvaté or polymorph thereof comprising a mono- or di-substituted indole group; said compound is selected from the group wherein:

Ri is H, R2 is F, Cl or OCH3 and R3 is H;

Ri is H, R₂ is F or Cl and R₃ is CH₃;

Ri is CH₃, R2 is OCH3 and R3 is H;

Ri is F, R2 is F and R3 is H;

Ri is CH3, R2 is F and R3 is H;

Ri is CF3 or OCF3 and R2 is H and R3 is H;

Ri is OCF3, R2 is OCH3 and R₃ is H or

Ri is OCF3 , R₂ is H and R3 is CH3 in the manufacture of a pharmaceutical composition for inhibiting the réplication of dengue virus(es) in a patient.
10. The use of a compound according to claim 9 wherein the pharmaceutical composition is for coadministration of the compound represented by the structural formula (I) to the patient with an additional therapeutic agent.
11. The use of claim 10 wherein said additional therapeutic agent is another antiviral agent.