OA13304A - Inhibition of HIV / AIDS infection by dichrostachys glomerata extract. - Google Patents

Description

2 013304

INHIBITION OF SIDS / AIDS INFECTION BY DICHROSTACHYS GLOMERATA EXTRACT

The present invention relates to the inhibition of HIV / AIDS infection by DICHROSTACHYS GLOMERATA extract

In general, if a pharmaceutical industry is aware of the therapeutic virtues of a plant, the processes and techniques used for the extraction of the active ingredient or active ingredient vary according to the objectives, For example, by distillation, the essential oils of a plant can be extracted, water or alcohol can be used to extract active ingredients depending on whether the latter is soluble in water or in alcohol.

We have discovered a plant whose extraction by decoction at 100 ° C is used to manufacture immediate-efficacy antiretroviral drug monotherapy on HIV / AIDS. The treatment of this medication is to lower the viral load (number of copies of viral RNA per milliliter of blood) to undetectable and restore the immune system destroyed by HIV and therefore the patient regains his perfect health. The natural origin of this active substance makes it a medication that escapes the possibility of resistance development on the part of HIV, (VIRUSD'IMMUNODEFICIENCE HUMAINE) RESPONSIBLE FOR AIDS LAMALADIE (SYNDROME OF IMMUNODEFICIENCEACQUISE) discovered by Professor LUC MONTAGNIER in 1981 013304 10 15 20

The technique we propose is the decoction at 100 ° C which has been used for a long time in the pharmacopoeia: the plant is bathed in water brought to boiling for 45 minutes and left for 6 hours.

We will expose the knowledge of the plant resource until obtaining the active ingredient that we call "APIVIRINE". The characteristics of the plant are as follows: -Scientific name: Dichrostachys glomerata (in the local language FON (national language of BENIN the name is)

- BADAWIN - English name: mimosa bell - Botanical family: mimosacea - Botanical source: tropical plant distributed throughout West Africa. - The extracted substance of the plant is acid - Chemotype: 0 to 500 meters of altitude in tropical region (Altitude to which the plant is very rich in this therapeutic property and nontoxic according to our research sites of the South auNord BENIN) - Maturity: The adult plant is useful at any time, only the young shoots of the early rainy seasons should be harvested at least two months after the first rains of the year. - Useful part of the plant: The aerial part of the plant (lehuppier or foliage) except branches with diameters greater than two centimeters. The natural ratio of leaves / branch during the rainy season must be respected (the plant is well 25

013304 bushy in the rainy season and it is the opposite in drought) - Description of the plant: plant characterized by pronounced thornbizarres, its leaves are very small, its flowers attract bees, it is a legume which gives pods 5 folded on herself. -Reproductibility on a large scale: This plant is easy to produce on a large scale, at the beginning of the rainy seasons the young shoots that stand out from the roots are very numerous and are easy to be transplanted 10 -TOXICITY: this medicinal plant is in no way toxic - For the harvest and storage of the plant: All branches of the plant (shrub that becomes a shrub in the long term) garnished with green leaves are good to be harvested. Leaves that start to yellow are to be removed (effect of drought). Once the harvest is done, all is stored in a building sheltered from the sun's rays, spread on the cemented floor or in concrete, the thickness of the stall should not exceed 15 cm otherwise the leaves rot. that these conditions are respected, the leaves, although dried, retain their therapeutic property. The extraction can be done immediately after harvest, if this is possible it is very good because the plant easily gives up its active ingredient.

For the extraction of the active ingredient apivirine the method isdécéction which is done according to the following steps:

The total quantity to be extracted must be weighed and marked. This step is very important because the quality (nontoxicity and antiretroviral potency: antiviral strengh) of the active ingredient is 013304 -5 dependent on the total weight of the boiled plant. During the weighing, branches of more than two centimeters must be removed, otherwise they influence the total weight and distort the TOTAL WEIGHT / QUALITY ratio of the active principle.

Then follows the cooking process which takes place in the following way: the water must float on the leaves and once the boiling is reached, it should last for forty-five (45) minutes, after that time we complete the water (because of the loss of water due to evaporation) and the intensity of the heating must be maintained until a new boil after the source of the heating has to be cut off and the leaves are left in the cooking water for 6 hours.

The leaves are removed, rinsed, the liquid resulting from the hardening is added to that of the extraction, and this liquid is used to make a new extraction and as many times as you want. We complete the water so that it floats the leaves. The rinsing must be rigorously done in order to recover the maximum of the active substance, the liquid of the last extraction is filtered to pass to the dehydration. NB: The advantage of using the same liquid to make newextractions is that there is a high concentration of the active ingredient in water and dehydration takes less time.

All the liquid obtained from the extraction obtained after filtration is subjected to heating for the purpose of complete dehydration, the aim being to obtain the active ingredient in its purest form. The liquid is evaporated to a black PH of between 3 and 4 and a moisture content of less than 10 percent (measured with a refractometer). Towards the end, the heating should be gentle and

013304 controlled in such a way that the substance will not be charred. The substance solidifies without any special intervention after cooling if the dehydration is perfect.

An important clarification: one hundred kilograms (100KG) of the treated plant gives 3100 grams of pure APIVIRINE of PH.situated .- between 3 and 4 and moisture content less than 10 percent. This pure concentrate of apivirine can be stored without further precautions and can be shipped anywhere in the world without difficulty. If the moisture content is not respected the substance will mold and this affects the quality of the finished product.

In hot water apivirine dissolves easily and can be exploited easily. NB: During dehydration the liquid tends to rise and fill up its container to overflow. Monitoring is done to prevent this overflow which causes the loss of molecules and which decreases the quantity of the active ingredient which must be extracted from the total quantity of plant.

Important clarification: The material used for decoction and dehydration must be stainless

Once the active ingredient in its pure form is obtained, any pharmaceutical industry can exploit it and transform it into a drug in various forms: syrup, tablet, capsule, oral ampoule, etc.

This antiviral substance has an exceptional impact on HIV, the effect is immediate if the daily dose is the size of the viral load of the patient. It is an active ingredient that ranks among

01 3304 r7 Antiretrovirals ANTIVIH / SIDA and especially natural origin.

A very important precision for the dosage for an adult patient: the daily dose of apivirine for an adult is molecular concentration from the extract of 970 grams of plants or 30 grams of apivirine divided into three daily doses after meals, the dose per dose is therefore 10 grams, the dose, beyond this limit, makes the stool hard for some people although having a superior antiviral strike force. It can be accepted to exceed this daily dose without reaching 15 grams per dose, this dosage (10 to 15 g / dose) is for the treatment of attack or to stop immediately persistent diarrhea related multiple infections or especially to destroy the maximum virus in a few days in to obtain an undetectable viral load in less than a month.

Any pregnant woman or nurse can consume this active ingredient without inconvenience

The dosage for children depends on the tolerance observed at sonniveau by the doctor, the recommended dose is much lower than 1/3 of that of adults and that is according to its age. As undesirable effects, this substance makes the saddles hard for certain people.

In case there are hard stools, the use of any laxative is recommended, the acidity of the apivirine syrup makes that some people tend to vomit just after taking, just dilute the syrup in a glass of water demi to avoid this effect. end 013304

For the duration of treatment, it must be continuous even if the viral load of the patient is undetectable (below the threshold of sensitivity of * '~ - ** the device that makes the viral load, today this threshold is 10 or 20 or 50 depending on the performance of the machine) and its health perfectly recovered, so for life according to our knowledge today.

HIV can not develop resistance against this active ingredient. This substance is 100 percent natural (nonsynthesized in the laboratory) and permanently retains its therapeutic property against HIV. The natural origin of its chemical composition gives it this power, it is not a molecule that is directed against the virus, but a set of all-antiviral molecules capable of inhibiting HIV infection. Certainly this composition varies in time and according to the seasons but remains effective on sensitive germs. The biological parameters that confirm the antiretroviral anti-HIV efficacy of apivirine are as follows: the viral load which is the medical examination that determines the efficacy and strength of antiviral resistance of any substance that affects HIV. It reveals at the moment T the number of existing viruses. This analysis is repeated every month or every two months to see the gradual decline in the number of copies of viral RNA per milliliter of blood to the undetectable result. Then, the second analysis is the CD4 or T4 lymphocyte count which is the medical examination which shows the restoration of the patient's immune system under anti-HIV treatment. This analysis is repeated every four or six months. This is counting the number of T cells per mm of blood.

Claims (9)

013304 CLAIMS 1. Use of the scientifically-named medicinal plant Dichrostachys glomerata for the manufacture of an antiretroviral medicament for the treatment of human immunodeficiency virus (HIV) infection causative agent of acquired immunodeficiency syndrom (AIDS). 2. The biological evidence for the efficacy of this Dichrostachys glomerata-based antiretroviral is demonstrated by the decrease in viral load to the undetectable result and the arrest of CD4 (T4) lymphocyte degeneration and subsequent restoration. 3. Use of the medicinal plant of scientific nameDichrostachys glomerata according to one of claims 1 and 2, in the form of an extract of said medicinal plant. 4. Use of the medicinal plant of scientific name Dichrostachys glomerata according to claim 3, in which said extract of said medicinal plant is obtained by the process of manufacture comprising at least: a. A step of decoction of the aerial part of the Dichrostachys glomerata plantemédicinale into water, which decoction step comprises: • a first phase of boiling at boiling temperature preferably for 45 minutes, • a phase of addition of a volume of d by replacing the volume of evaporated water during the first cooking phase, a second cooking phase to boiling and a resting phase without heating for 6 hours; b. A step of separating the aerial part of the Dichrostachys glomerata plantemédicinale from the liquid resulting from the decoction step, which separation step comprises: • removing the aerial part of the medicinal plantDichrostachys glomerata from the liquid resulting from the dedecoction step, • a first rinse of the aerial part of the Dichrostachys glomerata plantemédicinale by water to obtain a liquid from the first rinse, • the mixture of said liquid from the decoction step and liquid liquid from the first rinse, • a second rinse of said aerial part of the medicinal plant Dichrostachys glomerata by the product derived from said mixture so as to form liquid from the second rinsing, • the filtration of said liquid from the last rinsing to form a filtered liquid; c. A step of dehydrating said filtered liquid, which dehydration step comprises the sub-heating evaporation of said filtered liquid so as to obtain the extract of Dichrostachys glomerata, said evaporating or heating being maintained until the extracted color of Dichrostachys glomerata is black, the pHdudit extracted from Dichrostachys glomerata is between 3 and 4, and that the moisture content of said extract of Dichrostachys glomerata is less than 10%. 5. Use of the medicinal plant of scientific name Dichrostachys glomerata according to claim 4, in which said extract of said medicinal plant is obtained by the manufacturing process in which at least one additional rinsing of said aerial part of the medicinal plant Dichrostachysglomerata is carried out after the second rinse of said partial carrier of the medicinal plant Dichrostachys glomerata, each additional rinse being carried out from the liquid of the preceding rinse. 6. Use of the medicinal plant of scientific name Dichrostachys glomerata according to one of the preceding claims, wherein said medicament is in desirop form, tablet, capsule or oral ampoule in monotherapy. 7. Use of the medicinal plant of scientific nameDichrostachys glomerata according to one of the claims In the preceding embodiment, wherein said medicament is in the form of an adult patient comprising the daily dose of said extract is 30 grams divided into three doses. 8. Use of the medicinal plant of the scientific name Dichrostachys glomerata according to claim 6, in whichthe intake of said extract is 10 grams and should not exceed 15 grams. 9. Use of the medicinal plant of scientific name Dichrostachys glomerata according to one of the preceding claims, wherein said medicament is in the form ofprise for a child patient comprising the daily dose of extracted extract is 10 grams divided into three takes. The use of the scientifically named medicinal plant Dichrostachys glomerata according to claim 9, wherein the intake of said extract is 3.33 grams. 7