NZ754349B2 - Ectoparasitic Treatment Method and Composition - Google Patents
Ectoparasitic Treatment Method and CompositionInfo
- Publication number
- NZ754349B2 NZ754349B2 NZ754349A NZ75434914A NZ754349B2 NZ 754349 B2 NZ754349 B2 NZ 754349B2 NZ 754349 A NZ754349 A NZ 754349A NZ 75434914 A NZ75434914 A NZ 75434914A NZ 754349 B2 NZ754349 B2 NZ 754349B2
- Authority
- NZ
- New Zealand
- Prior art keywords
- thiacloprid
- animal
- sheep
- lice
- composition
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 86
- 239000005940 Thiacloprid Substances 0.000 claims abstract description 101
- HOKKPVIRMVDYPB-UVTDQMKNSA-N Thiacloprid Chemical compound C1=NC(Cl)=CC=C1CN1C(=N/C#N)/SCC1 HOKKPVIRMVDYPB-UVTDQMKNSA-N 0.000 claims abstract description 101
- 241001674048 Phthiraptera Species 0.000 claims abstract description 75
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- 230000002085 persistent Effects 0.000 claims description 14
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- YWTYJOPNNQFBPC-UHFFFAOYSA-N Imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 description 9
- 210000002268 Wool Anatomy 0.000 description 9
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- 241001113967 Bovicola ovis Species 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 206010061217 Infestation Diseases 0.000 description 7
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- 241000283690 Bos taurus Species 0.000 description 1
- 241000322475 Bovicola Species 0.000 description 1
- 241001283755 Bovicola caprae Species 0.000 description 1
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 description 1
- 241000257161 Calliphoridae Species 0.000 description 1
- XFXVOLYVSOVOSC-UHFFFAOYSA-N ClC1=CC=CC(=N1)CN1C(SCC1)=NC#N Chemical compound ClC1=CC=CC(=N1)CN1C(SCC1)=NC#N XFXVOLYVSOVOSC-UHFFFAOYSA-N 0.000 description 1
- FHIVAFMUCKRCQO-UHFFFAOYSA-N Diazinon Chemical compound CCOP(=S)(OCC)OC1=CC(C)=NC(C(C)C)=N1 FHIVAFMUCKRCQO-UHFFFAOYSA-N 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
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Abstract
The present invention relates to a method of controlling lice on an animal. The method comprises administering externally a pharmaceutically effective amount of thiacloprid. The present invention also relates to a composition comprising thiacloprid for use in controlling lice on an animal.
Description
ECTOPARASITIC TREATMENT METHOD AND COMPOSITION
TECHNICAL FIELD
This invention relates to an ectoparasitic treatment method and composition.
In particular, the present invention relates to the treatment of biting lice on sheep,
although it should be appreciated that aspects of the present invention can be
extended to related matters.
BACKGROUND ART
Ectoparasites of animals are a significant concern, affecting the health of the animal,
and the costs and productive output of farm animals. A particular problem for farmers
in Australia is parasite infestation on sheep.
In Australia, lice and blowflies are the two most significant external parasite problems
that affect sheep. Both parasite groups are the cause of extensive production losses
and serious animal welfare concerns. Lice are ‘obligate parasites’, meaning that they
only live on a host and can only survive for a relatively short time away from a host.
Lice are also said to be “species specific”, which means that generally, sheep lice only
live on sheep. Accordingly, goats, cattle, pigs and horses are each affected by their own
species of lice. Although sheep lice may survive on goats for a short period of time,
goats are not likely to be carriers of sheep lice. Although sheep may commonly be
affected by at least three species of lice, the most prevalent and most significant is the
biting louse, Bovicola (formerly Damalinia) ovis.
In Australia, lice are estimated to cost the wool industry more than $120 million per
year. These costs include expenditure on treatment and control of infestations as well
as production losses. Significant reductions in fleece value (e.g. up to 30%), greasy
fleece weight and clean fleece weight have all been demonstrated in lice-infested
17782756_1 (GHMatters) P93847.NZ.1
flocks (Wilkinson et al., 1982; Niven & Pritchard, 1985; Elliott et al., 1986; Cleland et
al., 1989). Lice infestation has also been shown to detrimentally affect the colour of
the fleece, i.e. making it less bright and more yellow (Kettle and Lukies, 1982), and can
result in the downgrading of skins due to ‘cockle’ (Heath, 1995a).
The impacts on animal welfare and production due to sheep lice (e.g. B. ovis) are a
consequence of the irritation experienced by infested sheep. Primarily, the sheep
show signs of pruritus (itchiness) such as rubbing, biting and scratching and these
behaviours damage the integrity of the fleece (e.g. causing ‘pulled’ or cotted wool). In
some sheep the pruritus can be intense and the irritation can manifest as changes to
the skin, including increased scurf and thickening of the epidermis and overlying lipid
layer (Britt et al. 1986; Heath et al. 1995b). There is variation between individual sheep
susceptibility to lice and this may be linked to an immune response to infestation
(James 1999; James et al. 2002).
B. ovis are recognised as feeding on “skin scurf, lipids, loose stratum corneum squames
and bacteria” (James, Moon & Brown, 1998). Although most lice (particularly adults)
have been observed in the fleece, away from the skin surface, they are quite mobile
and are likely to feed on substrates other than just the loose debris present at that
location (Sinclair, Butler & Picton, 1989). The feeding behaviour of B. ovis at skin level
appears to occur only on the surface of the epidermis and does not bring it into direct
contact with the internal tissues or blood of the host. Observations have determined
that B. ovis “does not ingest nucleated keratinocytes and apparently does not
penetrate deeper than the outer layers of the stratum corneum” (James, Moon &
Brown, 1998).
Lice are transmitted between sheep via direct contact.
17782756_1 (GHMatters) P93847.NZ.1
The control of lice requires effective chemical treatment of an entire flock and
subsequent biosecurity measures to prevent re-infestation (i.e. exclusion of lice-
infested sheep).
External administration of ectoparasiticides to sheep is usually most effective when
applied immediately after shearing (off-shears). In particular, treatment by backlining
either by pour-on or spray on is recommended to be applied within 24 hours of
shearing, although within 7 days may be suitable for some products.
Treatment using ‘dipping’ administration methods can generally be used on longer
wool than backlining. These methods include plunge dipping, shower dipping and cage
dipping. Administration is recommended within 2 weeks of shearing, although up to 6
weeks after shearing may also be suitable. Treatment of sheep with long wool may
require hand-jetting or a specialised long-wool backlining treatment.
Not all parasiticidal actives are suitable for all of these different types of
administration, due to various reasons including mode of action, safety, formulation
requirements, persistency and potency.
The administration of any chemical parasiticide will to some degree result in residue
on the animal. If a smaller quantity of parasiticide can be used, the resultant residues
may also be lower. For high volume applications such as dipping or jetting, residues in
the environment may also be reduced by using lower concentrations of suitable
actives. However, the actives must maintain efficacy at lower concentration to be
suitable. The use of lower quantities of actives is also desirable to lower cost and/or
potentially decrease safety issues. Lower residues are desired in that they may provide
shorter withhold times, avoid export limitations, and increase market acceptability.
In Australia the common types of externally administered chemical parasiticides
suitable for the treatment and/or prevention of sheep lice include insect growth
17782756_1 (GHMatters) P93847.NZ.1
regulators (IGRs), such as triflumuron; spinosyns, such as spinosad; macrocyclic
lactones, such as ivermectin; magnesium fluorosilicate; organophosphates ; synthetic
pyrethroids; and the neonicotinoid imidacloprid.
IGRs do not affect adult lice, resulting in a delay of up to 14 weeks for the developing
stages to be killed and for the adult lice to die off. There have also been reports of
emerging resistance to IGRs in Australia.
Spinosyns have low toxicity and provide rapid knock-down, but have a short duration
of action.
Macrocyclic lactones also provide rapid knock-down, but are required to be
administered in relatively large quantities to be effective.
Organophosphates are under increased scrutiny in regard to safety. In Australia the
use of diazinon, the most commonly used organophosphate for control of sheep lice,
has been discontinued since May 2007.
Synthetic pyrethroids can take 6-8 weeks to kill lice, which may lead to further
spreading of lice across a flock. Significant resistance has also occurred with synthetic
pyrethroids, being reported since the 1980s. The resistance has progressed to the
extent that the use of synthetic pyrethroids in many locations may no longer be
effective in the control of sheep lice.
In Australia, imidacloprid is the active ingredient in the parasiticide product Avenge™,
sold by Bayer Australia Ltd, for the control of lice on sheep. Avenge™ contains
imidacloprid at a concentration of 3.5% w/v, and is applied as a backline treatment.
When used for lice control on sheep, Avenge™ provides up to four weeks of persistent
activity when applied within 24 hours following shearing, which can prevent
reinfestation by lice.
17782756_1 (GHMatters) P93847.NZ.1
There is a clear need for alternative methods for controlling ectoparasites such as
sheep lice. Ideally such methods would use an active with high potency against lice to
allow for relatively low quantities and concentrations of actives during use, be suitable
for both off-shears (short wool) and long wool treatments, and have high persistency.
It is an object of the present invention to address one or more of the foregoing
problems and/or to at least provide the public with a useful choice.
All references, including any patents or patent applications cited in this specification
are hereby incorporated by reference. No admission is made that any reference
constitutes prior art. The discussion of the references states what their authors assert,
and the applicant reserves the right to challenge the accuracy and relevance of the
cited documents.
It will be clearly understood that, although a number of prior art publications are
referred to herein, this reference does not constitute an admission that any of these
documents form part of the common general knowledge in the art, in Australia or New
Zealand or in any other country.
Throughout this specification, the word “comprise”, or variations thereof such as
“comprises” or “comprising”, will be understood to imply the inclusion of a stated
element, integer or step, or group of elements integers or steps, but not the exclusion
of any other element, integer or step, or group of elements, integers or steps.
Further aspects and advantages of the present invention will become apparent from
the ensuing description which is given by way of example only.
17782756_1 (GHMatters) P93847.NZ.1
DISCLOSURE OF THE INVENTION
The invention is based in part on the surprising discovery that thiacloprid has high
potency and suitability for the control of lice, when applied externally to an animal.
Thiacloprid is as shown in the chemical formula:
Thiacloprid: [3-[(6-Chloropyridinyl)methyl]thiazolidinylidene]cyanamide is an
insecticide of the neonicotinoid class.
The present invention provides the following items 1 to 28:
1. A method of controlling lice on an animal, characterised by the step of
administering externally a pharmaceutically effective amount of thiacloprid.
2. The method of item 1, characterised by the step of topically administering a
composition containing from 0.0001% to 3.5% w/v of thiacloprid.
3. The method of item 1 or 2, wherein the amount of thiacloprid administered to
the animal is from 1 to 45 mg/kg by weight of the animal.
4. The method of item 3, wherein the amount of thiacloprid administered to the
animal is from 10 to 30 mg/kg by weight of the animal.
. The method of any one of items 1 to 4, wherein the method includes the step of
diluting a composition containing from 30% to 60% w/v of thiacloprid with water
before administering the diluted composition to the animal.
6. The method of item 5, wherein the composition is diluted with water to provide
from 1 ppm to 200 ppm of thiacloprid.
17782756_1 (GHMatters) P93847.NZ.1
7. A method of providing persistent control of lice on an animal, characterised by
the step of administering externally a pharmaceutically effective amount of
thiacloprid.
8. The method of item 7, wherein persistent control is provided for at least two
weeks following administration.
9. The method of any one of items 1 to 8, wherein the thiacloprid is administered
to the animal by backlining, dipping or jetting.
. Use of thiacloprid in the manufacture of a composition for controlling lice on an
animal.
11. The use of item 10, wherein the composition contains from 0.0001% to 60% w/v
of thiacloprid.
12. The use of item 11, wherein the composition contains from 0.0001% to 3.5% w/v
of thiacloprid.
13. The use of item 11, wherein the composition contains from 30% to 60% w/v of
thiacloprid.
14. The use of any one of items 10 to 13, wherein the amount of thiacloprid
administered to the animal is from 1 to 45 mg/kg by weight of the animal.
. The use of item 14, wherein the amount of thiacloprid administered to the
animal is from 10 to 30 mg/kg by weight of the animal.
16. The use of any one of items 10 to 15, wherein the composition is administered to
the animal by backlining, dipping or jetting.
17. Use of thiacloprid in the manufacture of a composition for persistent control of
lice on an animal.
18. The use of item 17, wherein the persistent control is provided for at least two
weeks following administration.
17782756_1 (GHMatters) P93847.NZ.1
19. A composition comprising thiacloprid for use in controlling lice on an animal.
. The composition of item 19, wherein the composition contains from 0.0001% to
60% w/v of thiacloprid.
21. The composition of item 20, wherein the composition contains from 0.0001% to
3.5% w/v of thiacloprid.
22. The composition of item 20, wherein the composition contains from 30% to 60%
w/v of thiacloprid.
23. The composition of any one of items 19 to 22, wherein the amount of thiacloprid
administered to the animal is from 1 to 45 mg/kg by weight of the animal.
24. The composition of item 23, wherein the amount of thiacloprid administered to
the animal is from 10 to 30 mg/kg by weight of the animal.
. The composition of any one of items 19 to 24, wherein the composition is
administered to the animal by backlining, dipping or jetting.
26. A composition comprising thiacloprid for use in persistent control of lice on an
animal.
27. The composition of item 26, wherein the persistent control is provided for at
least two weeks following administration.
28. The method of any one of items 1 to 9, the use of any one of items 10 to 18, or
the composition of any one of items 19 to 27, wherein the animal is a sheep or a
goat.
17782756_1 (GHMatters) P93847.NZ.1
The present invention as claimed herein is described in the following items 1 to 8:
1. A method of controlling lice on an animal, characterised by the step of topically
administering a pharmaceutically effective amount of thiacloprid, wherein the
amount of thiacloprid administered to the animal is from 10 to 30 mg/kg by
weight of the animal, wherein the thiacloprid is administered in a composition
containing 0.5%-2% w/v of thiacloprid, and wherein the animal is a sheep or a
goat.
2. The method of item 1, wherein the composition is administered to the animal by
backlining.
3. The method of item 1 or 2, wherein the composition contains 1% w/v of
thiacloprid.
4. The method of any one of items 1 to 3, wherein the amount of thiacloprid
administered to the animal is from 10 to 15 mg/kg by weight of the animal.
. The method of any one of items 1 to 4, wherein the method provides persistent
control of lice on the animal.
6. The method of item 5, wherein persistent control is provided for at least two
weeks following administration.
7. The method of any one of items 1 to 6, wherein the animal is a sheep.
8. The method of item 1 substantially as herein described with reference to any one
of the Examples.
MODES FOR CARRYING OUT THE INVENTION
Throughout the specification, the use of the present invention will be particularly
described in relation to the control of biting lice on sheep (Bovicola ovis). However, it
should be appreciated that the principles in the present invention could apply to and
be useful in other situations – for instance controlling biting lice (Bovicola caprae) on
17782756_1 (GHMatters) P93847.NZ.1
goats. Many of the problems associated with sheep are also present in goats having
regard to their long hair and related physiological systems.
According to an aspect of the present invention there is provided a method of
controlling lice on an animal, characterised by the step of administering externally a
pharmaceutically effective amount of thiacloprid.
According to a further aspect of the invention there is provided a method of
controlling lice on an animal, characterised by the step of topically administering a
composition containing from 0.0001% to 3.5% w/v of thiacloprid.
According to a preferred aspect of the invention the amount of thiacloprid
administered to the animal is from 1 to 45 mg/kg by weight of the treated animal.
More preferably the amount of thiacloprid administered to the animal is from 5 to 40
mg/kg by weight of the treated animal. Even more preferably the amount of
thiacloprid administered to the animal is from 10 to 30 mg/kg by weight of the treated
animal.
According to a preferred aspect of the invention the composition administered in the
method of controlling lice on an animal contains from 0.1% to less than 3.5% w/v of
thiacloprid. More preferably, the composition administered in the method of
controlling lice on an animal contains from 0.5% to 2% w/v of thiacloprid. Preferably
the composition is administered as a backline treatment. More preferably the
composition is administered to a sheep within 7 days after shearing.
According to another preferred aspect of the invention the composition administered
in the method of controlling lice on an animal contains from 1 mg/L (ppm) to 200 mg/L
(ppm) of thiacloprid. More preferably, the composition administered in the method of
controlling lice on an animal contains from 5 mg/L (ppm) to 100 mg/L (ppm) of
thiacloprid. Even more preferably, the composition administered in the method of
17782756_1 (GHMatters) P93847.NZ.1
controlling lice on an animal contains from approximately 48 mg/L (ppm) of
thiacloprid.
Preferably the composition is administered as a dip treatment. More preferably the
composition is administered to a sheep within 6 weeks after shearing.
Preferably the method includes the steps of diluting a composition containing from
% to 60% w/v of thiacloprid with water before administering to the animal. More
preferably the composition is diluted with water to provide from 1 ppm to 200 ppm of
thiacloprid.
According to another preferred aspect of the invention the composition administered
in the method of controlling lice on an animal contains from 100 mg/L (ppm) to 1000
mg/L (ppm) of thiacloprid. More preferably, the composition administered in the
method of controlling lice on an animal contains from 200 mg/L (ppm) to 600 mg/L
(ppm) of thiacloprid. Preferably the composition is administered as a jetting treatment.
Preferably the composition is administered to a sheep after 2 weeks or more after
shearing. More preferably the composition is administered to a sheep after 4 weeks or
more after shearing.
According to another aspect of the present invention there is provided a method of
providing persistent control of lice on an animal, the method including the step of
administering topically a composition including thiacloprid. Preferably the method
provides persistent protection for at least two weeks following administration.
According to another aspect of the present invention there is provided a composition
for controlling lice on an animal, characterised by including thiacloprid. Preferably the
composition includes from 0.0001% to 60% w/v thiacloprid.
17782756_1 (GHMatters) P93847.NZ.1
According to another aspect of the present invention there is provided a composition
for use in a method of controlling lice on an animal, the composition including 30% to
60% w/v of thiacloprid. A particularly preferred composition includes in the order of
48% w/v thiacloprid.
According to another aspect of the present invention there is provided a composition
for use in a method of controlling lice on an animal, the composition including 0.3% to
less than 3.5% w/v of thiacloprid. More preferably, the composition administered in
the method of controlling lice on an animal contains from 0.5% to 2% w/v of
thiacloprid. A particularly preferred composition includes in the order of 1% w/v
thiacloprid.
According to another aspect of the present invention there is provided a composition
for use in a method of controlling lice on an animal, the composition including 1 mg/L
(ppm) to 200 mg/L (ppm) of thiacloprid. More preferably, the composition
administered in the method of controlling lice on an animal includes from 5 mg/L to
100 mg/L of thiacloprid. A particularly preferred composition includes in the order of
48 mg/L of thiacloprid.
According to another aspect of the present invention there is provided a composition
for use in a method of controlling lice on an animal, the composition including 100
mg/L (ppm) to 1000 mg/L (ppm) of thiacloprid. More preferably, the composition
administered in the method of controlling lice on an animal includes from 200 mg/L to
600 mg/L of thiacloprid. A particularly preferred composition includes in the order of
400 mg/L of thiacloprid.
The medicament can be administered to the animal (e.g. a sheep) by any suitable
means, including but not limited to backlining, dipping, or jetting. In some
embodiments, the composition is applied to all or a majority of the fleece or hair of the
17782756_1 (GHMatters) P93847.NZ.1
animal. In some embodiments, the composition is applied by localised topical
application to part of the fleece or hair of the animal, e.g. to the back of the animal.
It should be appreciated that the present invention can be combined with other active
ingredients, for instance as combined formulations or combination treatments. Other
suitable actives include but are not limited to other parasiticides, anthelmintics,
vaccines, vitamins/mineral supplements and the like. Not only does this offer
advantages by having two or more treatments being delivered in a single dose, but
they may also be synergistic effects resulting. For example, the inclusion of vitamins
and minerals may assist the skin recovery of an animal once the biting lice have
disappeared as a result of the insecticide treatment.
In preferred formulations, in addition to the active, there may be provided dispersing
agents, wetting agents, surfactants, emulsifiers, solvents, co-solvents, thickeners, anti-
oxidants, stabilisers, preservatives, anti-foaming agents, colourants and humectants.
Preferred methods of administration are discussed below. These methods are
described with particular reference to administration to sheep, since sheep are the
most typical species that is treated, however one skilled in the art will be aware of
suitable adaptations and means for administering to other animals.
Dipping
A traditional form of dipping for sheep is known as plunge dipping, wherein the sheep
are mustered through a deep trough containing the treatment liquid. The sheep swim
through the liquid allowing the liquid to penetrate into the fleece. The sheep may also
be completely submerged into the liquid by a handler immersing each sheep’s head
into the liquid.
An alternative method of dipping is known as shower dipping, wherein the sheep are
sprayed with the treatment liquid from overhead nozzles and optionally from side
17782756_1 (GHMatters) P93847.NZ.1
spraying nozzles. A typical arrangement uses a circular treatment enclosure with a
rotating boom arm with downwards spraying nozzles mounted above the enclosure.
The treatment time is approximately 12 minutes for the sheep to be sufficiently
wetted with the treatment liquid.
Jetting
The jetting procedure involves the use of a high pressure stream or jet of treatment
fluid being directed onto the animal. The application technique includes manual hand-
jetting and the use of an automated jetting race. Manual hand-jetting uses a pump to
supply treatment fluid to a jetting-wand held by an operator, who administers the
treatment to the sheep. The jetting-wand includes a spray head with a nozzle or
nozzles for directing the liquid into the fleece of the sheep, which helps saturate areas
of the fleece and provide skin contact with the fluid. The operator uses the jetting
wand to apply the liquid as onto different areas of the animal’s body. The areas for
administration may be chosen by the operator depending on the circumstances, such
as wool length, and the requirements for different animals such as rams and wethers.
The pressure of the jets is typically around 700 kPa, and the minimum volume of
treatment liquid is around 0.5 L per month of wool growth.
An automated jetting race is a less laborious method of jetting compared to hand-
jetting, using apparatus that applies treatment liquid to the back of an animal as it
passes under a series of nozzles while traveling through the apparatus. Generally a
higher volume and lower pressure of treatment liquid is used, compared to hand
jetting.
Backlining
The backlining technique, otherwise referred to as pour-on or spray-on, is the
application of a relatively small amount of liquid to back of an animal. The liquid may
be applied as a spray or a stream or jet of liquid, using manual applicators or power
assisted applicators. The volume, concentration viscosity and other properties of the
17782756_1 (GHMatters) P93847.NZ.1
treatment liquid may vary depending on the requirements. Typically a pour-on is
administered in two or more bands along either side of the spine of the sheep.
The inventors have surprisingly discovered that thiacloprid has unexpectedly high
efficacy in the control of lice on sheep.
One particular advantage provided by the present invention is the long persistency
following administration. For instance, when thiacloprid is administered as a plunge-
dip and/or pour-on, four weeks of persistency has been measured.
There is limited understanding in the prior art of the usefulness of thiacloprid in
controlling parasites on animals.
The superior potency of thiacloprid compared to imidacloprid in the control of lice on
sheep is a surprising result. The dose of imidacloprid recommended for the product
AVENGE™ for the control of lice on sheep is around 49 mg/kg of the body weight of
the sheep. By comparison, thiacloprid has been demonstrated as effective when used
as a pour-on treatment at a dose as low as 5 mg/kg thiacloprid.
In one embodiment, the present invention provides a method of controlling lice on
sheep, characterised by the step of administering externally thiacloprid in an amount
of from 5 to 40 mg/kg by weight of the treated animal, wherein the thiacloprid is
administered by localised topical application to the back of the animal. In one
embodiment, the thiacloprid is administered as a pour-on. In one embodiment, the
thiacloprid is administered as one or more bands along either side of the spine of the
animal.
In one embodiment of the invention, the high potency of thiacloprid is utilised by the
provision of a 1% w/v composition of thiacloprid for use as a backline treatment for
17782756_1 (GHMatters) P93847.NZ.1
lice control on sheep. By comparison, the backline imidacloprid formulation Avenge™,
uses the substantially higher concentration of 3.5% w/v.
This significantly higher potency allows for lower concentrations and/or quantities of
thiacloprid to be used compared to similar parasiticides such as imidacloprid. It can be
seen that the present invention has a number of advantages over the prior art,
including lower residues both on the animal and the environment, lower costs for
manufacture, ease of manufacture, and a lower chance of lice developing resistance.
EXAMPLES
In table 1 is a formulation example of the present invention. In particular this
formulation may be used as a concentrate that is diluted with water for use in dipping,
such as plunge dipping, cage dipping or spray dipping. Typically this formulation would
be diluted to around 48 ppm for use in dipping.
Table 1: Formulation of thiacloprid concentrate
Formulation 1
Ingredients Proportion
Thiacloprid 48% Active
Polysorbate 80 10-15% Surfactant
Plasdone K25 2.0-5.0% Dispersing
Xanthan gum 0.1- 0.3% Thickening
Wacker silica 0.5- 1.5 Thickening/ dispersing
Methyl paraben 0.2 Preservative
Propyl paraben 0.04 Preservative
Defoamer RD 0-0.1 Anti foam
Propylene glycol 10- 20% Anti-freeze
Deionised water Qs to 100%
17782756_1 (GHMatters) P93847.NZ.1
In table 2 is an example composition of the invention with particular suitability for use
in a backlining treatment, such as a pour-on.
Table 2: Formulation of thiacloprid pour-on
Formulation 2
Ingredients Proportion
Thiacloprid 1.0% Active
DMA (Dimethyl acetamide) 40% Solvent
BHT 0.2% Antioxidant
Dipropylene glycol methyl ether Qs to 100% Co-solvent
Trial Example 1
Six sheep with a natural infestation of B. ovis were treated by hand jet with a 400 mg/L
(ppm) solution of thiacloprid (approx. 4L per sheep). At 166 days after treatment
average numbers of live lice on these sheep were reduced by 96.4%.
Trial Example 2
Six sheep infested with B. ovis were treated with 400 mg/L (ppm) thiacloprid by hand
jet (approx. 5L per sheep). Lousicidal efficacy (calculated using geometric means and
the Henderson-Tilton formula) was 99.5% at 104 days after treatment.
Trial Example 3
Subsequent studies were conducted in which various concentrations of thiacloprid
were assessed for lousicidal efficacy when applied by hand jet to naturally-infested
sheep.
Seven (7) different concentrations ranging from 300 ppm down to 20 ppm were
assessed in these studies. A positive correlation between thiacloprid concentration and
efficacy was observed.
17782756_1 (GHMatters) P93847.NZ.1
Trial Example 4
In this study, sheep with a natural infestation of lice were treated with aqueous
solutions of thiacloprid ranging from 500 to 125 ppm, applied as a plunge dip. All
sheep treated at all dose levels were found to be free from live lice at 20 weeks after
treatment (WAT).
Trial Example 5
A subsequent study was performed in which lice-infested sheep were treated by
plunge dip with various concentrations of thiacloprid from 120 to 15 ppm. Lousicidal
efficacy was high at all concentrations.
Trial Example 6
A second dose-titration plunge dip study was conducted using six thiacloprid
concentrations of around 15, 8, 4, 2, 1 and 0.5 ppm. Acceptable levels of efficacy were
demonstrated at the three highest concentrations tested in this trial.
Trial Example 7
The efficacy of a concentration of 48 ppm was further confirmed when treatment of
six lice-infested sheep successfully eliminated live lice from detection, when the sheep
were assessed over a period of 12 months.
Trial Example 8
An in vitro assessment to compare the relative potencies of imidacloprid and
thiacloprid was conducted by means of an adult louse bioassay. The results of this
study indicated that thiacloprid was almost six times more potent than imidacloprid
against a field strain of B. ovis when compared at the lethal concentration 50% (LC50)
level.
17782756_1 (GHMatters) P93847.NZ.1
Trial Example 9
Another unexpected finding following plunge dip treatment with thiacloprid at 48 ppm
was the persistence of lousicidal efficacy. In two studies, introduction of lice to treated
sheep up to 4 weeks after treatment did not result in successful establishment of new
infestations. This presents a significant advantage over other plunge dip products
currently available for use on sheep in Australia for which such persistent lousicidal
efficacy is not known to occur.
Trial Example 10
A study was performed using 10 g/L thiacloprid pour-on formulation, on four groups of
sheep at different dose rates including a negative control group, as summarised in
table 3. The results of the study demonstrated that thiacloprid 10 g/L pour-on
effectively controlled a natural infestation of sheep lice when applied at a dose level as
low as 0.5 mL per kg bodyweight (approximately 200 mg thiacloprid per square metre
of sheep body surface area). At each assessment time, including 22 weeks after
treatment, there was an absence of live lice on all three groups.
Table 3: Trial of 1% w/v thiacloprid pour-on
Volume Nominal Number of Assessment
Group Treatment
administered Dose level Sheep times
Thiacloprid
1 1.5 ml/kg 600 mg/m 8
g/L Pour-On
Thiacloprid Pre-shearing,
2 1.0 ml/kg 400 mg/m 7
g/L Pour-On 4, 8, 12 and
Thiacloprid 22 weeks post
3 0.5 ml/kg 200 mg/m 8
g/L Pour-On treatment
Negative
4 Nil Nil 7
controls
Aspects of the present invention have been described by way of example only and it
should be appreciated that modifications and additions may be made thereto without
departing from the scope thereof as defined in the appended claims.
17782756_1 (GHMatters) P93847.NZ.1
REFERENCES
Wilkinson, F.C., de Chaneet, G.C. and Beetson, B.R. (1982). Growth of populations of
lice, Damalinia ovis, on sheep and their effects on production and processing
performance of wool. Veterinary Parasitology 9, 243-252 (Wilkinson et al, 1982).
Niven, D.R. and Pritchard, D.A. (1985). Effects of control of the sheep body louse
(Damalinia ovis) on wool production and quality. Australian Journal of Experimental
Agriculture 25, 27-31 (Niven and Pritchard, 1985).
Elliott, J., Jones, A.L. and Pauley, J.R. (1986). The effect of body lice on wool
production. Australian Advances in Veterinary Science. Australian Veterinary
Association, pp 125-126 (Elliott eat al., 1986).
Cleland, P.C., Dobson, K.J. and Meade, R.J. (1989). Rate of spread of sheep lice and
their effects on wool quality. Australian Veterinary Journal 66, 298-299 (Cleland et al.,
1989).
Kettle, P.R. and Lukies, J.M. (1982b). Effects of sheep lice Damalinia ovis on wool
colour. New Zealand Journal of Experimental Agriculture 10, 15-17 (Kettle and Lukies,
1982).
Heath, A.C.G., Cooper, S.M., Cole, D.J.W. and Bishop, D.M. (1995). Evidence for the
role of the sheep biting louse Bovicola ovis in producing cockle, a sheep pelt defect.
Veterinary Parasitology 59, 53-58 (Heath, 1995a).
Britt, A.G., Cotton, C.L., Pitman, I.H. and Sinclair, A.N. (1986). Effects of the sheep-
chewing louse (Damalinia ovis) on the epidermis of the Australian Merino. Australian
Journal of Biological Science 39, 137-143 (Britt et al, 1986).
17782756_1 (GHMatters) P93847.NZ.1
Heath, A. C. G., Cole, D. J. W., Bishop, D. M., Pfeffer, A., Cooper, S. M., Risdon, P.
(1995). Preliminary investigations into the aetiology and treatment of cockle, a sheep
pelt defect. Veterinary Parasitology 56, 239-254 (Heath, 1995b).
James, P.J. (1999). Do sheep regulate the size of their mallophagan louse populations?
International Journal for Parasitology 29, 869-875 (James 1999).
James, P.J., Carmichael, I.H., Pfeffer, A., Martin, R.R. and O’Callaghan, M.G. (2002a).
Variation among Merino sheep in susceptibility to lice (Bovicola ovis) and association
with susceptibility to trichostrongylid gastrointestinal parasites. Veterinary
Parasitology 103, 355-365 (James et al., 2002).
James, P.J., Moon,R.D. and Brown, D.R. (1998). Seasonal dynamics and variations
among sheep in densities of the sheep biting louse, Bovicola ovis. International Journal
for Parasitology 28, 283-292 (James, Moon & Brown, 1998).
Sinclair, A.N., Butler, R.W. and Picton, J. (1989). Feeding of the chewing louse
Damalinia ovis (Shrank) (Phthiraptera:Trichodectidae) on sheep. Veterinary
Parasitology 30, 233-251 (Sinclair, Butler & Picton, 1989).
17782756_1 (GHMatters) P93847.NZ.1
Claims (8)
1. A method of controlling lice on an animal, characterised by the step of topically administering a pharmaceutically effective amount of thiacloprid, wherein the amount of thiacloprid administered to the animal is from 10 to 30 mg/kg by 5 weight of the animal, wherein the thiacloprid is administered in a composition containing 0.5%-2% w/v of thiacloprid, and wherein the animal is a sheep or a goat.
2. The method of claim 1, wherein the composition is administered to the animal by backlining. 10
3. The method of claim 1 or 2, wherein the composition contains 1% w/v of thiacloprid.
4. The method of any one of claims 1 to 3, wherein the amount of thiacloprid administered to the animal is from 10 to 15 mg/kg by weight of the animal.
5. The method of any one of claims 1 to 4, wherein the method provides persistent 15 control of lice on the animal.
6. The method of claim 5, wherein persistent control is provided for at least two weeks following administration.
7. The method of any one of claims 1 to 6, wherein the animal is a sheep.
8. The method of claim 1 substantially as herein described with reference to any 20 one of the Examples. 17782756_1 (GHMatters) P93847.NZ.1
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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AU2013902122A AU2013902122A0 (en) | 2013-06-12 | Ectoparasitic Treatment Method and Composition | |
AU2013902122 | 2013-06-12 | ||
NZ71449914 | 2014-06-12 |
Publications (2)
Publication Number | Publication Date |
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NZ754349A NZ754349A (en) | 2021-06-25 |
NZ754349B2 true NZ754349B2 (en) | 2021-09-28 |
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