NZ742693B2 - Rice grain with thickened aleurone - Google Patents

Rice grain with thickened aleurone Download PDF

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Publication number
NZ742693B2
NZ742693B2 NZ742693A NZ74269316A NZ742693B2 NZ 742693 B2 NZ742693 B2 NZ 742693B2 NZ 742693 A NZ742693 A NZ 742693A NZ 74269316 A NZ74269316 A NZ 74269316A NZ 742693 B2 NZ742693 B2 NZ 742693B2
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NZ
New Zealand
Prior art keywords
grain
ros1a
rice plant
polypeptide
rice
Prior art date
Application number
NZ742693A
Other versions
NZ742693A (en
Inventor
Crispin Alexander Howitt
Philip John Larkin
Chun Ming Liu
Jinxin Liu
Xiao Ba Wu
Ronald Chun Wai Yu
Original Assignee
Commonwealth Scientific And Industrial Research Organisation
Institute Of Botany Chinese Academy Of Sciences
Filing date
Publication date
Application filed by Commonwealth Scientific And Industrial Research Organisation, Institute Of Botany Chinese Academy Of Sciences filed Critical Commonwealth Scientific And Industrial Research Organisation
Priority claimed from PCT/AU2016/051106 external-priority patent/WO2017083920A1/en
Publication of NZ742693A publication Critical patent/NZ742693A/en
Publication of NZ742693B2 publication Critical patent/NZ742693B2/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
    • A01H1/101—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine or caffeine
    • A01H1/102—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine or caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/121—Plant growth habits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4636—Oryza sp. [rice]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/115—Cereal fibre products, e.g. bran, husk
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The present invention relates to rice grain with thickened aleurone. Also provided is a rice plant comprising at least one genetic variation which reduces the activity of at least one ROS1a gene in the plant. Grain of the invention, or aleurone therefrom, has improved nutritional properties, and hence is particularly useful for human and animal feed products.

Claims (41)

1. Grain of a rice plant, the grain comprising an aleurone, a y endosperm, a ROS1a gene encoding a ROS1a polypeptide, wherein the amino acid sequence of the ROS1a polypetide is at least 95% identical to SEQ ID NO: 2, wherein the ROS1a gene comprisesone or more genetic variations when compared to a corresponding wild-type rice plant, whereby the aleurone is thickened ed to ne from a corresponding wild-type grain, and wherein the one or more genetic variations(s) comprise an introduced c modification in the ROS1a gene.
2. The grain of claim 1, which is further terised by one or more or all of: (a) the ROS1a polypeptide has DNA glycosylase activity; (b) the ROS1a polypeptide is a variant of a corresponding wild-type ROS1a polypeptide in that their amino acid sequences are different; (c) the ROS1a polypeptide has a level of DNA ylase activity which is between 2% and about 60% of the level of DNA glycosylase ty of a corresponding wild-type ROS1a polypeptide and/or of ROS1a polypeptide whose amino acids have a sequence set forth in SEQ ID NO: 2; (d) a level of ROS1a polypeptide between 2% and about 60% is present in the grain compared to the level of ROS1a polypeptide in the corresponding wild-type grain; and (e) the aleurone is thickened compared to aleurone from a corresponding ype grain and comprises at least two, at least three, at least four or at least five layers of cells, about 3, about 4, about 5 or about 6 layers of cells, or 2-8, 2-7, 2-6 or 2-5 layers of cells.
3. The grain of claim 1 or claim 2, wherein the ROS1a gene produces a reduced level of a wild-type ROS1a polypeptide, for example which comprises a splice-site mutation that s in a reduced level of expression of the ROS1a gene, relative to the wild-type ROS1a gene whose cDNA sequence is provided as SEQ ID NO:8, or wherein the ROS1a gene comprises a mutation in its promoter which results in reduced expression of the ROS1a gene relative to the wild-type ROS1a gene.
4. The grain according to any one of claims 1 to 3, n the rice plant is characterised by one or more or all of: (a) the rice plant has a level of DNA glycosylase activity in its developing grain which is between 2% and about 60% of the level of DNA glycosylase activity in a corresponding wildtype developing grain; (b) the activity of at least one ROS1a gene in the rice plant is reduced in one or more or all of aleurone, rp, nucellar projection, ovary, testa and starchy endosperm of the developing grain; (c) the ty of a ROS1a gene is reduced at least at a time point between the time of anthesis and 7 days post-anthesis, and/or in the egg cell prior to anthesis; (d) the rice plant is male and female fertile; and (e) the rice plant exhibits delayed grain maturation.
5. The grain according to any one or more of claims 1 to 4, which is further characterised by one or more of: (a) the grain comprises, when compared to a corresponding ype grain, one or more or all of the following, each on a weight basis, i) a higher mineral content, preferably the mineral content is the t of one or more or all of zinc, iron potassium, magnesium, phosphorus and sulphur, ii) a higher antioxidant content, iii) a higher phytate content, iv) a higher content of one or more or all of vitamins B3, B6 and B9, v) a higher dietary fibre content and/or insoluble fibre content, vi) a starch content which is between about 90% and about 100% by weight relative to the starch content of the corresponding wild-type grain; vii) a higher sucrose content, viii) a higher monosaccharide content, and ix) a lipid content of at least 90% or at least 100% relative to the lipid content of the corresponding wild-type grain, (b) the grain comprises an ; (c) the grain is whole grain or cracked grain; (d) the grain has been processed so that it is no longer able to germinate, preferably by heat treatment; (e) the grain has a germination rate which is between about 70 and about 100% relative to the germination rate of a corresponding wild-type grain; (f) the grain comprises an increased proportion of amylose in its total starch content ed to the corresponding wild-type grain; and (g) the grain comprises an increased proportion of oleic acid and/or a decreased proportion of palmitic acid in its total fatty acid content compared to the ponding wild-type grain.
6. The grain according to any one or more of claims 1 to 5, which is characterised by one or more or all of: (a) the grain comprises a ROS1a gene which encodes a ROS1a polypeptide which has DNA glycosylase ty, preferably in one or more of aleurone, testa and starchy endosperm of the grain, wherein the ROS1a polypeptide which has DNA glycosylase activity is preferably a mutant ROS1a polypeptide; (b) the grain comprises a mutant ROS1a polypeptide having decreased DNA glycosylase activity when expressed in the rice plant compared to a corresponding ype ROS1a polypeptide, ably wherein the mutant ROS1a ptide comprises one or more amino acid substitutions, deletions or insertions which reduces DNA glycosylase activity compared to the corresponding wild-type ROS1a polypeptide; and (c) the grain has a reduced total amount of ROS1a polypeptide compared to a corresponding wild-type grain, preferably reduced in one or more of aleurone, testa and starchy endosperm of the grain, provided that the grain ses at least one ROS1a gene which encodes a ROS1a polypeptide which has DNA glycosylase activity.
7. The grain according to any one of claims 1 to 6, which is pigmented in its outer s).
8. The grain according to any one of claims 1 to 7, wherein the ROS1a polypeptide comprises amino acids whose ce is at least 99% identical to SEQ ID NO: 2, or the ROS1a polypeptide(s) comprises amino acids whose sequence is at least 99% identical to SEQ ID NO: 2 and which sequence is different to the amino acid sequence of the corresponding wild-type ROS1a ptide.
9. An isolated and/or exogenous polynucleotide encoding a ROS1a polypeptide whose amino acid sequence is at least 95% identical to SEQ ID NO: 2 and which has reduced, preferably no, DNA glycosylase activity when compared to the ponding wild-type ROS1a polypeptide, wherein a rice plant comprising the polynucleotide produces grain having a thickened aleurone compared to the aleurone from a corresponding ype grain.
10. An isolated and/or exogenous polynucleotide which, when present in a rice plant, reduces the expression of a ROS1a gene, wherein a rice plant comprising the isolated and/or exogenous cleotide produces grain having a thickened aleurone compared to the aleurone from a corresponding wild-type grain.
11. The polynucleotide of claim 10, when used for reducing the expression of a ROS1a gene in developing grain of a rice plant at least at a time point between the time of is and 7 days post-anthesis.
12. A nucleic acid construct and/or vector encoding a polynucleotide according to any one of claims 9 to 11, wherein the nucleic acid construct or vector comprises a DNA region encoding the polynucleotide operably linked to a promoter which is expressed in developing grain of a rice plant at least at a time point n the time of is and 7 days post-anthesis.
13. A recombinant cell sing an exogenous polynucleotide according to any one of claims 9 to 11, or a nucleic acid construct and/or vector of claim 12.
14. The cell of claim 13, wherein the exogenous polynucleotide, nucleic acid construct or vector is integrated into the genome of the cell, preferably into the nuclear genome.
15. A cell of a rice plant comprising a ROS1a gene encoding a ROS1a polypeptide, wherein the amino acid sequence of the ROS1a polypeptide is at least 95% identical to SEQ ID NO: 2, wherein the ROS1a gene comprises one or more genetic variations when compared to a corresponding wild-typ ROS1a gene, and y a rice plant sing a ROS1a gene encoding the ROS1a polypeptide produces grain having a thickened aleurone compared to the aleurone from a corresponding wild-type grain.
16. The cell of claim 15 which is an aleurone, pericarp, nucellar projection, ovary, testa or y endosperm cell.
17. A rice plant which produces grain according to any one of claims 1 to 8, a polynucleotide according to any one of claims 9 to 11, a nucleic acid construct and/or vector of claim 12 and/or which comprises a cell according to any one of claims 13 to 16.
18. A method of producing a rice plant of claim 17 or genetically modified grain therefrom, the method comprising the steps of i) introducing into a rice cell, an exogenous cleotide according to any one of claims 9 to 11, or a nucleic acid construct and/or vector of claim 12, ii) obtaining a genetically modified rice plant from a cell obtained from step i), the genetically modified rice plant being genetically modified for the exogenous polynucleotide, nucleic acid construct or vector, and iii) optionally harvesting grain from the plant of step ii), the grain being genetically modified for the exogenous polynucleotide, c acid construct or vector, and iv) optionally producing one or more generations of cally modified progeny plants from the genetically modified grain, the y plants being transgenic for the exogenous polynucleotide, nucleic acid construct or , thereby producing the rice plant or cally modified grain.
19. A method of producing a rice plant of claim 17 or grain therefrom, the method comprising the steps of i) introducing into a rice cell, a mutation of an endogenous ROS1a gene such that the mutated ROS1a gene s a ROS1a polypeptide whose amino acid sequence is at least 95% identical to SEQ ID NO: 2 and is different to the amino acid sequence of a corresponding wildtype ROS1a polypeptide, or does not encode a ROS1a polypeptide, ii) obtaining a rice plant from a cell obtained from step i), the rice plant comprising the on of the endogenous ROS1a gene, iii) screening the plant of step ii) for the production of grain according to any one of claims 1 to 8, and iv) optionally harvesting grain from the plant of step ii), the grain comprising the mutation of the endogenous ROS1a gene, and v) optionally producing one or more tions of progeny plants from the grain, the progeny plants comprising the mutation of the endogenous ROS1a gene, thereby producing the rice plant or grain.
20. A method of selecting a rice plant of claim 17 or rice grain ing to any one of claims 1 to 8, the method comprising the steps of i) screening a population of rice plants or grain each of which were obtained from a mutagenic ent of itor rice cells, grain or plants, for the production of grain according to any one of claims 1 to 8 or for the presence of a mutation in a ROS1a gene, or the presence of rice grain according to any one of claims 1 to 8, and ii) selecting from the population of step (i) a rice plant which es grain according to any one of claims 1 to 8 or which comprises a mutant ROS1a gene, or rice grain of step (i) which is rice grain according to any one of claims 1 to 8, thereby selecting the rice plant or grain.
21. A method of selecting a rice plant of claim 17, the method comprising the steps of i) producing one or more progeny plants from rice grain, the rice grain having been derived from a cross of two parental rice , ii) screening the one or more progeny plants of step i) for the production of grain according to any one of claims 1 to 8, and iii) selecting a progeny plant which produces the grain, thereby selecting the rice plant.
22. The method of claim 21, wherein step ii) ses one or more or all of: i) analysing a sample comprising DNA from a y plant for the genetic variation, ii) analysing the thickness of aleurone of grain obtained from a progeny plant, and iii) analysing the nutritional t of the grain or a part thereof.
23. The method of claim 21, wherein step iii) comprises one or more or all of: i) selecting a progeny plant which is homozygous for the genetic variation, wherein the genetic variation reduces DNA glycosylase activity in the rice plant when compared to a corresponding wild-type rice plant, ii) selecting a progeny plant whose grain has an increased aleurone thickness ed to a corresponding wild-type grain, iii) selecting a progeny plant whose grain or a part thereof has an altered nutritional content compared to a corresponding wild-type grain or part thereof.
24. The method according to any one of claims 21 to 23 which further comprises i) crossing two parental rice , preferably wherein one of the parental rice plants produces grain according to any one of claims 1 to 8, or ii) backcrossing one or more progeny plants from step i) with plants of the same genotype as a first parental rice plant which does not produce grain according to any one of claims 1 to 8 for a sufficient number of times to produce a plant with a majority of the genotype of the first parental rice plant but which produces grain according to any one of claims 1 to 8, and iii) selecting a progeny plant which produces grain according to any one of claims 1 to 8.
25. Use of an exogenous polynucleotide according to any one of claims 9 to 11, or a nucleic acid uct and/or vector of claim 12, to produce a recombinant cell, a transgenic rice plant or transgenic grain.
26. The use of claim 25 when used to produce rice grain according to any one of claims 1 to
27. A method for identifying a rice plant which produces grain according to any one of claims 1 to 8, the method comprising the steps of i) obtaining a nucleic acid sample from a rice plant, and ii) screening the sample for the presence or absence of a genetic variation which reduces the activity of a ROS1a gene in the plant when compared to a corresponding wild-type rice plant.
28. The method of claim 27, n the genetic variation is one or both of a) a nucleic acid construct sing a polynucleotide, or the polynucleotide encoded thereby, which when present in a rice plant reduces the expression of a ROS1a gene, and b) a gene, or mRNA encoded thereby, which expresses a mutant ROS1a polypeptide with reduced ROS1a polypeptide activity.
29. The method of claim 27 or claim 28, wherein the presence of the genetic variation indicates that grain of the rice plant has a thickened aleurone when compared to a corresponding rice plant lacking the genetic variation(s).
30. A method for identifying a rice plant which produces grain according to any one of claims 1 to 8, the method sing the steps of i) obtaining grain from a rice plant, and ii) ing the grain or a n thereof for one or more of a) a thickened aleurone, b) the amount of ROS1a polypeptide and/or activity in the grain, and c) the amount of mRNA encoded by ROS1a genes in the grain.
31. The method of according to any one of claims 27 to 30 which identifies a rice plant of claim 21.
32. A method of producing rice flour, bran, wholemeal, malt, starch or oil obtained from grain, the method comprising; a) obtaining grain according to any one of claims 1 to 8, and b) sing the grain to produce the flour, bran, wholemeal, malt starch or oil.
33. A product produced from grain according to any one of claims 1 to 8, or a rice plant of claim 17, or from a part of said grain or rice plant.
34. The product of claim 33 comprising one or more or all of the ROS1a gene, the c variation, the exogenous nucleic acid construct and the thickened ne.
35. The t of claim 33 or claim 34, wherein the part is bran.
36. The product according to any one of claims 33 to 35, wherein the product is an ingredient in a food, an ingredient in a beverage, a product that is a foodstuff or a product that is a ge.
37. The product of claim 36, wherein i) the ient in food or the ingredient in a beverage is selected from the group consisting of wholemeal, flour, bran, starch, malt and oil, ii) the product that is a uff is selected from the group consisting of: leavened or unleavened breads, pasta, s, animal fodder, ast cereals, snack foods, cakes, pastries and foods containing a flour-based sauce, or iii) the product that is a beverage is a packaged beverage or a beverage comprising ethanol.
38. A method of preparing the ingredient in a food or beverage of claim 36 or claim 37, the method comprising processing grain according to any one of claims 1 to 8, or bran, flour, wholemeal, malt, starch or oil from the grain, to produce the food or beverage ingredient.
39. A method of preparing the product that is a foodstuff or the product that is a beverage of claim 36 or claim 37, the method comprising mixing grain according to any one of claims 1 to 8, or bran, flour, wholemeal, malt, starch or oil from the grain, with another food or beverage ingredient.
40. Use of grain according to any one of claims 1 to 8 or part thereof, or a rice plant of claim 17 or part thereof, as animal feed or food, or to produce feed for animal consumption or food for human consumption.
41. A composition comprising one or more of a polynucleotide according to any one of claims 9 to 11, a nucleic acid construct and/or vector of claim 12, or a cell according to any one of claims 13 to 16, and one or more able carriers. None set by JYK MigrationNone set by JYK Unmarked set by JYK None set by JYK ionNone set by JYK Unmarked set by JYK QxONm qu?m wamq excomqm 239,: £39,: Atmgvo?m??m?omo 2:82. 239,: 232,: $382. R39: 3:57: oomHHmHOWO 339,: 3:9,: 2.382. mama; 239,: 839 omeHmHOmo :5 N382. R392. mcinmE @539: gm min”: ac: Nmmn: mc?cmsamm<2o ¢quq¢w oomHHmHOmo
NZ742693A 2016-11-17 Rice grain with thickened aleurone NZ742693B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2015904754A AU2015904754A0 (en) 2015-11-18 Rice grain with thickened aleurone
PCT/AU2016/051106 WO2017083920A1 (en) 2015-11-18 2016-11-17 Rice grain with thickened aleurone

Publications (2)

Publication Number Publication Date
NZ742693A NZ742693A (en) 2023-11-24
NZ742693B2 true NZ742693B2 (en) 2024-02-27

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