NZ554037A

NZ554037A - Pentacyclic anti-tumour compounds with angeloyl groups and optional sugar moieties

Info

Publication number: NZ554037A
Application number: NZ554037A
Authority: NZ
Inventors: Pui-Kwong Chan; May Sung Mak; Yun Wang
Original assignee: Pacific Arrow Ltd
Priority date: 2004-09-07
Filing date: 2005-09-07
Publication date: 2011-04-29

Abstract

Disclosed is the use of a compound of formula (5), or a salt or ester thereof, wherein the substituents are as defined in the specification, for treating ovarian cancer. Also disclosed are new compounds of the same formula and uses thereof. Also discloses is a method for isolating the above compounds from Xanthocera sorbifolia herb or plants from the sapindaceae family.

Description

<div class="application article clearfix" id="description"> Dkt. 804-H-PCT ANTI-TUMOR COMPOUNDS WITH ANGELOYL GROUPS This application is a Continuation-ln-Part of U.S. Serial No. 11/131,551, filed May 17, 5 2005, a Continuation-ln-Part of U.S. Serial No. 11/117,760, filed April 27, 2005, Continuation-ln-Part application of U.S. Serial No. 10/906,303, filed February 14, 2005, Continuation-ln-Part application of International Application No. PCT/US04/43465, filed December 23, 2004, which is a Continuation-ln-Part application of Int'l App'l No. PCT/US04/33359, filed October 8, 2004, which claims the benefit of U.S. Serial Nos. 10 60/532,101, filed December 23, 2003, and 60/509,851, filed October 9, 2003; and which claims the benefit of U.S. Serial Nos. 60/617,379, filed October 8, 2004, 60/613,811, filed September 27, 2004, and 60/607,858, filed September 7, 2004, 60/675,282, Filed April 27, 2005, and 60/675,284, Filed April 27, 2005. The contents of these preceding applications are hereby incorporated in their entireties by reference into this application. 15 Throughout this application, various publications are referenced. Disclosures of these publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains. FIELD OF THE INVENTION 20 This invention relates to novel anti-tumor compounds obtainable from Xanthoceras sorbifolia and plants from the sapindaceae family. BACKGROUND OF THE INVENTION Wenguanguo is a species of the sapindaceae family. Its scientific name is Xanthoceras 25 sorbifolia Bunge. Wenguanguo is the common Chinese name. Others are Wenguannguo, Wenguanmu, Wenguanhua, Xilacedeng, Goldenhorn and Yellowhorn. Wenguanguo is grown in Liaoning, Jilin, Hebei, Shandong, Jiangsu, Henan, Shanxi, Shaanxi, Gansu, Ningxia and Inner Mongolia, China. Its seeds, leaves and flowers are edible and have been used as a folk or traiditional medicine for centuries. Its branches 30 and woods are also used as a folk or traditional medicine. For more detailed information and background or relevent art of the present invention, please refer to page 1, lines 25-38, to page13 of International PCT Application No. PCT/US04/33359, filed October 8, 2004, and U.S. Serial No. 10/906,303, filed February 14, 2005. The contents of these -1 - WO 2006/029221 PCT/US2005/031900 pretfedirig appligations afe'Tiereoy incorporated in their entireties by reference into this application. Yingjie Chen, Tadahiro Takeda and Yukio Ogihara reported in Chem. Pharm. Bull 5 33(4)1387-1394(1985) describes a study on Xanthoceras sorbifolia Bunge. See Section V. Saponins from the Fruits of Xanthoceras sorbifolia. Four new saponin compounds were isolated from the fruits of Xanthoceras sorbifolia Bunge. These structures are bunkankasaponins A, B, C and D. Yingjie Chen, Tadahiro Takeda and Yukio Ogihara reported in Chem. Pharm. Bull 33(3)1043-1048(1985) is another a study focused on the 10 Xanthoceras sorbifolia Bunge. See Section IV. Structures of the Miner Prosapogenin. Yingjie Chen, Tadahiro Takeda and Yukio Ogihara. Chem. Pharm. Bull 33(1)127-134(1985) describes yet another study on the Xanthoceras sorbifolia Bunge. See Section III. Minor Prosapogenins Saponins from the Fruits of Xanthoceras sorbifolia Bunge. Other studies which have focused on saponin compounds include: Laurence 15 Voutquenne, Cecile Kokougan. Catherine Lavaud, Isabelle Pouny, Marc Litaudon.'Triterpenoid saponins and Acylated prosapogenins from Harpullia austro-caledonica." Phytochemistry 59 (2002) 825-832. Laurence Voutquenne et al. , "Haemolytic acylated triterpenoil saponins from Harpullia austro-caledonica" Phytochemistry 66(2005)825-835. Zhong Jaing, Jean-francois Gallard, Marie-Therese 20 Adeline, Vincent Dumontet, Mai Van Tri, Thierry Sevenet, and Mary Pais "Six Triterpennoid Saponins from Maesa laxiflora." .J. Nat. Prod. (1999), 62, 873-876. Young Seo, John M. Berger, Jennine Hoch, Kim M Neddermann, Isia Bursuker, Steven W. Mamber and David G. Kingston. "A new Triterpene Saponin from Pittosporum viridiflorum from the Madagascar Rainforest". J. Nat. Prod. 2002, 65, 65-68. Xiu-Wei 25 Yang, Jing Zhao, Xue-Hui Lui, Chao-Mei Ma, Masao Hattori, and Li He Zhang "Anti-HIV-1 Protease Triterpenoid Saponins from the Seeds of Aesculus chinensis." J. Nat. Prod. (1999), 62, 1510-1513. Yi Lu, Tatsuya Umeda, Akihito Yagi, Kanzo Sakata, Tirthankar Chaudhuri, D.K. Ganguly, Secion Sarma. "Triterpenoid Saponins from the roots of the tea plant (Camellia sinensis var. Assamica)." Phytochchemistry 53 (2000) 30 941-946. Sandra Apers, Tess E. De Bruyne, Magda Claeys, Arnold J. Viletinck, Luc A.C. Pieters. "New acylated triterpenoid saponins from Maesa laceceolata." Phytochemistry 52 (1999) 1121-1131. Ilaria D'Acquarica, Maria Cristina, Di Giovanni, Francesco Gasparrini, Domenico Misiti, Claudio D' Arrigo, Nicolina Fagnano, Decimo Guarnieri, Giovanni lacono, Giuseppe Bifulco and Raffaele Riccio. "Isolation and 35 structure elucidation of four new triterpenoid estersaponins from fruits of the -2- WO 2006/029221 PCT/US2005/031900 Pittfet^oi^bifitfebM^T^-T^traheeifon 58 (2002) 10127-10136. The contents of the above-mentioned references are hereby incorporated by reference. However, these references do not disclose saponin compounds having the same 5 structure as the compounds of this invention which are capable of inhibiting cancer or tumor cell growth. Cancer cells are defined by two heritable properties: (1) they reproduce in defiance of normal restraints on cell division; and (2) they invade and colonize territories normally reserved for other cells. Cancers require mutations of one to many genes for its 10 development, and they are classified according to the tissue and cell type from which they arise. Cancers arising from epithelial cells are named carcinomas; those arising from connective tissue or muscle cells are named sarcomas. In addition, there are cancers called leukemias, which are derived from hemopaietic cells. Cancers can also develop from cells of the nervous system. 15 Ovarian cancer is the 5th leading cause of cancer death in women, and the leading cause of death from gynecologic malignancies. In the United States, females have a 1.4 to 2.5%, or 1 out of 40-60 women, lifelong chance of developing ovarian cancer. Older women are at highest risk. More than half of the deaths from ovarian cancer occur in 20 women between 55 and 74 years of age, and approximately one quarter of ovarian cancer deaths occur in women between 35 and 54 years of age. See MedlinePlus Encyclopedia on ovarian cancer at http://www.nlm.nih.gov/medlineplus/ency/article/000889.htm. 25 Ovarian cancer is disproportionately deadly for a number of reasons. First, symptoms are vague and non-specific, so women and their physicians frequently attribute them to more common conditions. By the time the cancer is diagnosed, the tumor has often spread beyond the ovaries. Also, ovarian cancers shed malignant cells that frequently implant on the uterus, bladder, bowel, and lining of the bowel wall (omentum). These 30 cells can begin forming new tumor growths before cancer is even suspected. Second, because no cost-effective screening test for ovarian cancer exists, more than 50 percent of women with ovarian cancer are diagnosed in the advanced stages of the disease. -3- WO 2006/029221 PCT/US2005/031900 Thereror^.-'it'ifS'-aiVeTbjfeel 3T tnis! invention to provide compounds and/or compositions extracted from Xanthoceras sorbifolia or plants, or chemically or biochemically synthesized, which have substantial potency against ovarian cancer. 5 SUMMARY OF THE INVENTION In accordance with these and other objects of the invention, a brief summary of the present invention is presented. Some simplifications and omission may be made in the following summary, which is intended to highlight and introduce some aspects of the present invention, but not to limit its scope. Detailed descriptions of a preferred 10 exemplary embodiment adequate to allow those of ordinary skill in the art to make and use the invention concepts will follow in later sections. The invention provides six novel compounds of the structure (Y1, Y2, Y or Y3, Y8, Y9, Y10) as shown in Figure 1. As used herein, "Structure Y" is also referred to as -4- WO 2006/029221 PCT/US2005/031900 The formula, chemical name and common name of these compounds are presented in Table 1 below. 5 Table 1. Formula, Chemical Name and Common Name of Six Novel Compounds of Structure (Y, Y1, Y2, Y8, Y9, Y10) Names Formula Chemical Name Xanifolia-Y(Y3) C57H88O23 3-0-[(3-D-galactopyranosyl(1->2)]-a-L-arabinofuranosyl(1->3)-p-D-glucuronopyranosyl-21,22-0-diangeloyl-3p, 15a, 16a, 21 p, 22a, 28-hexahydroxyolean-12-ene, Xanifolia-Y1 C65H100O27 3-0-[P-D-galactopyranosyl(1-»2)]-a-L-arabinofuranosyl(1-»3)-p-D-glucuronopyranosyl-21-0-(3,4-diangeloyl)-a-L-rhamnophyranosyl-22-0-acetyl-3p,16a, 21 p, 22a, 28-pentahydroxyolean-12-ene Xanifolia-Y2 C57H88O24 3-0-[P-D-glucopyranosyl-( 1 ->2)]-a-L-arabinofuranosyl( 1 ->3)-(-D-glucuronopyranosyl-21,22-0-diangeloyl-3/?, 15a, 16a, 21/?, 22a, 24/?, 28-heptahydroxyolean-12-ene Xanifolia-Y8 C57H87O23 3-0-[/?-galactopyranosyl (1-^2)]-a-arabinofuranosyl (1-»3)-/?-glucuronopyranosyl-21, 22-0-diangeloyl-3/?, 16a, 21/?, 22a, 28-pentahydroxyolean-12-ene Xanifolia-Y9 C65H100O27 3-0-[/?-galactopyranosyl (1 ->2)]-a-arabinofuranosyl (1->3)-^-glucuronopyranosyl-21-0-(3,4-diangeloyl)-or-rhamnopyranosyl-28-0-acetyl-3/?, 16a, 21 22a, 28-pentahydroxyolean-12-ene Xanifolia-Y10 C57H87O22 3-0-[/?-galactopyranosyl (1^2)]-a-arabinofuranosyl (1->3)-y0-glucuronopyranosyl-21, 22-0-diangeloyl-3/?, 16a, 21/?, 22a, 28-pentahydroxyolean-12-ene The above six compounds (Y, Y1, Y2, Y8, Y9 and Y10) have anti-cancer effect. These 10 compounds inhibit the growth of human ovarian and other cancer cells. See Figure 2, 3 and 4. A concensus sub-structure was derived or identified from the bioactive compounds (Y, Y1, Y2, Y8, Y9 and Y10) of the invention. A concensus sub-structure of the compounds 15 of the invention is a biangeloyl group located on adjacent carbons. For example, in structure Y, Y2, Y8 and Y10, the biangeloyl group is located at 21 p and 22a of the triterpene backbone. See Figure 5. For structure Y1 and Y9, the biangeloyl group is -5- WO 2006/029221 PCT/US2005/031900 lociflefehdfc C$%irrftHP^rahi-Mi^Ming. See Figure 6. In an embodiment, the biangeloyl group of the bioactive compounds (Y, Y1, Y2, Y8, Y9 and Y10) of the invention is situated in a trans-position in adjacent carbons of a structure. See Figure 7. In another embodiment, a sugar moiety or sugar chain is located at C3 of the triterpene. In a 5 further embodiment, the sugar moiety or sugar chain selected from the group consisting of: D-g5ucose, D-galactose, L-rhamnose, L-arabinose, D-xylose, alduronic acid, D-glucuronic acid and D-galacturonic acid. Studies on the structural and functional relationship of the six compounds of the 10 invention indicate that changes to or substitutions of the functional groups located at C15 and C24 of the triterpene do not affect anticancer activity. The compounds (Y, Y1, Y2, Y8, Y9 and Y10) of the invention have activity in inhibition of turmor growth. See Figure 2, 3 and 4. The compounds are prepared by a chromatograpy purification process involving FPLC and HPLC as described in Figure 8, 9, 10, 11, 12 and 13. 15 Compound Y is purified using a procedure described in this application. Figure 11A shows the NMR peaks of compound Y.Comparision of Figure 2 and Figure 14 shows that the purified compound Y possesses potency (IC50 =1.5 ug/ml) 10 times higher than the original extract (IC50 = 25ug/ml). Compound Y also has a high selectivity toward ovarian cancer. See Figure 15. 20 The purified compound Y1, Y2, Y8, Y9, and Y10 also show inhibitory activity toward human cancer cells with a higher potency toward ovarian carcinoma. See Figure 3 and 4. The plant extract containing compound Ys also shows inhibitory activity toward human cancer cells. For example, studies were performed on eleven human cancer cell 25 lines, and the plant extract of the invention shows a higher potency toward ovarian carcinoma. See Figures 14, 15 and 16 and Table 3.1 for a comparison of the activities of the plant extract of the invention among the different cancer cell lines. As used herein, "Ys" or "compound Ys" is used to denote compound Y or Y3, Y1, Y2, Y8, Y9, Y10, or other bioactive compounds obtainable from a Xanthoceras sorbifolia extract of 30 the invention. This invention provides an extract of Xanthoceras sorbifolia capable of inhibiting cancer growth. The cancer includes, but is not limited to ovary cancer, bladder cancer, prostate cancer, leukocytes cancer, and bone cancer. Compounds of the invention, shown to be 35 effective against cancer, can be isolated from the plant called Xanthoceras sorbifolia, WO 2006/029221 PCT/US2005/031900 synthesized enemicaiiy, ^extracted from other biological sources, including plants from the sapindaceae family. This invention provides a process of producing bioactive compounds from husks, 5 leaves, branches or stems, fruit-stems, seed shell, roots and barks of the Wenguanguo. The extraction of the bioactive compounds of the invention from different parts of the Wenguangu plant can be performed separately or simultaneously. This invention further discloses methods of obtain the bioactive compounds of the invention. In addtion to saponin comopunds, the extracts of the invention also contain saccharides, proteins, 10 glycosides, flavonoids, curmarin extracts, alkaloid extracts, organic acid extracts, tannin and other bioactive compounds. The saponin compounds obtainable from the extract of the invention were investigated, and have been shown to possess inhibitory activity against cancer growth. 15 The compounds, extracts or compositions of the present invention is capable of regulating many cellular pathways, including the receptors or components of a cell, such as G-protein receptor, Fas protein, receptor Tyrosine Kinases, Mitogen, mitogen receptor. The compounds of the invention can be isolated from the plant called Xanthoceras sorbifolia, synthesized chemically, or extracted from other biological 20 sources, including plants from the sapindaceae family. This invention provides compounds, including compound of structures Y, Y1, Y2, Y8, Y9 and Y10, obtainable from Xanthoceras sorbifolia and capable of inhibiting cancer growth. In an embodiment, the cancer includes but is not limited to bladder cancer, 25 cervix cancer, prostate cancer, lung cancer, breast cancer, leukocytes cancer, colon cancer, liver cancer, bone cancer, brain cancer, and ovary cancer. This invention provides a compound of oleanene triterpenoidal saponin comprising a side chain at Carbon 21 and Carbon 22 of said compound, wherein the side chain comprises angeloyl groups. In an embodiment, the compound comprises one or more sugars, 30 wherein C3 and C4 of the sugar are acylated with angeloyl groups. This invention provides a triterpinoidal saponin compound comprising a triterpene backbone and a biangeloyl group, wherein the one angeloyl group is attached to 21 p and one angeloyl group is attached to 22a of the triterpene backbone, and wherein the presence of the biangeloyl group produces anticancer activity. This invention provides a triterpenoidal 35 saponin compound comprising a triterpene backbone and a sugar moiety or rhamnose, WO 2006/029221 PCT/US2005/031900 wherein the sugar-moieTy'or rnamnose is attached to the triterpene backbone, wherein the sugar moiety or rhamnose further comprises a biangeloyl group, and wherein the presence of the biangeloyl group produces anticancer activity. This invention provides a triterpenoidal saponin compound comprising a triterpene backbone, said triterpene 5 backbone is acylated at either 21 p or 22a position or at both 21 p and 22a position with a sugar moiety or sugar chain, and wherein at least one sugar in the sugar moiety or sugar chain comprises angeloyl groups attached to the C3 and C4 position of said sugar. In an embodiment, the two angeloyl groups are in a trans-position on a structure, and the presence of the biangeloyl group produces anticancer activity. In an 10 embodiment, the two angeloyl groups are in a trans-position in adjacent carbons on a structure, and the presence of the biangeloyl group produces anticancer activity. This invention provides a salt of the above-described compounds. This invention provides a pharmaceutical composition comprising an effective amount of the above-described compounds and a pharmaceutical^ acceptable carriers). 15 This invention provides a method for isolating compounds from Xanthoceras sorbifolia comprising the steps of: extracting Xanthoceras sorbifolia powder with an appropriate amount of an organic solvent for an appropriate amount of time to obtain an extract, identifying the bioactive compounds in the extract; purifying the bioactive compounds in 20 the extract with FPLC to obtain a fraction of the bioactive compounds; and isolating the purified individual bioactive compound from the fraction of the bioactive compounds with preparative HPLC. This invention provides a compound having a structure verified by NMR spectral data derived from proton NMR, carbon NMR, 2D NMR of the Heteronuclear Multiple Quantum Correlation (HMQC), Heteronuclear Multiple Bond 25 Correlation (HMBC), NOESY and COSY, and Mass spectral data derived from MALDI-TOF and ESI-MS. This invention provides a compound and its derivatives which are effective against cancer. The compounds or compositions of the present invention are capable of 30 regulating various cellular pathways, including but not limiting the following: receptors or components, such as G-protein receptor, Fas protein, receptor for Tyrosine Kinases, mitogens, mitogen receptors, TGF Beta-smad, FGF, TGF-beta and TGF-alpha, ras- GTPase-MAP kinase, jun-fos, Src-fyn, Jak-Jnk-STAT, BMP, Wnt, or myc-cell proliferation. The compounds and composition of the invention derivied from 35 Xanthoceras Sorbifolia is also capable of regulating the components and receptors -8- WO 2006/029221 PCT/US2005/031900 associatea- wirn- ceirueatrir anu ciit: uctpctule of (re)activating the cell death program or pathways. DETAILED DESCRIPTION OF THE FIGURES 5 Figure 1 shows the structures of six bioactive anticancer saponin compound of the invention. Figure 2 shows the anticancer activity of purified Compound Y, The experiment was performed on ovarian cancer cells (OCAR-3) and the inhibition activity was determined by MTT assay. For details, refer to Experiment 3. Abscissa: Concentration (ug/ml). 10 Ordinate: % Cell Growth. The IC50 is approximately 1-1.5 ug/ml. A: Point scale. B: Linear scale. Figure 3 shows the inhibition of the purified Compound Y1 and Compound Y2 on the growth of ovarian cancer cells. Figure 4 shows the anticancer activity of Y, Y8, Y9 and Y10 on ovarian cancer cells as 15 determined by MTT assay. Figure 5 shows a consensus structure derived from four bioactive anticancer saponin compounds of the invention (i.e., Y, Y2, Y8 and Y10). Figure 6 shows a consensus structure derived from two bioactive anticancer saponin compounds of the invention (i.e., Y1 and Y9). 20 Figure 7 shows a general structural formular derived from the consensus structures of the six bioactive compounds of the invention (i.e., Y, Y1, Y2, Y8, Y9 and Y10). (A) A consensus active functional group is the biangeloyl group attached to 21 p and 22a of the triterpene backbone. (B) A consensus active functional group is the biangeloyl group attached at C3 and C4 of a sugar ring (or rhamnose). In an embodiment, the 25 functional active structure is a biangeloyl group situated in a trans-position on a structure. Figure 8 shows the separation of the components of Xanthoceras sorbifolia extract by HPLC with a ^bondapak C18 column. Details of the experiment were presented in Experiment 2. 30 Figure 9 shows the elution profile of an extract of Xanthoceras sorbifolia in FPLC with 10-80% gradient. Ordinate: Optical density (at 245nm). Abscissa: Fractions (5ml/fraction). Figure 10 shows the results of the screening of cell growth activity using fractions obtained from FPLC chromatography. The assay was conducted in bladder cells. The 35 fractions obtained from FPLC as shown in Figure 9 were used. As shown in this figure, -9- WO 2006/029221 PCT/US2005/031900 difterent'components OT^anxncxjeras sorbifolia extracts cause either growth or inhibition effects on cells. Only fraction 5962 (Fraction Y) causes cell inhibition. Fractions 610, 1116 and 1724 cause minor stimulation of cell growth. Abscissa: concentration (ug/ml). Ordinate: % Cell Growth (determined by MTT assay). 5 Figure 11 shows HPLC profile of Fraction Y with 45% acetonitrile isocratic elution in a preparative C18 column (Delta Pak C18). Under these conditions, fractions Y (Y3), Y1 and Y2 are well separated from each other and they are subsequently purified. A and B shows the purity of the collected Y3 and Y2 by HPLC under same conditions. Figure 12 shows the separation profile of Y8, Y9 andY10 with 45% acetonitrile isocratic 10 elution in a preparative C18 column (Delta Pak C18). Figure 13 shows the HPLC profiles of purified Y8, Y9 and Y10. Figure 14 shows the growth curves of ovarian cancer cells after treatment with the crude extract of Xanthoceras sorbifolia as determined by the MTT assay. The two curves in the figure are two experiments results. This study determined the efficacy of 15 the extract of Xanthoceras sorbifolia on cancer cells. In these experiments, cancer cell lines from 11 different human organs were employed. This figure shows that ovary cancer cells are the most sensitive cancer cells in responding to Xanthoceras Sorbifolia extract of the invention. Results of other cancer cells were represented in Figures 16A, 16B, 16C. Abscissa: concentration (ug/ml). Ordinate: % Cell Growth (determined by 20 MTT assay). Figure 15 shows the comparison of potency of Compound Y in ovarian cancer cells and cervical cancer cells. Ovarian cancer cells are much more sensitive than the cervical cancer cells. The IC50 for Compound Y in ovary cells is about 1.5ug/ml while the IC50 in cervical cancer cells is over 20 ug/ml. 25 Figures 16A shows the growth curves of sensitive group of bladder and bone cancer cells as determined by MTT assay. The curves in the figure are the repeated experiments results. Abscissa: concentration (ug/ml). Ordinate: % Cell Growth (determined by MTT assay). Figures 16B shows the growth curves of semi-sensitive group: leukocyte, liver; 30 prostate, breast and brain cancer cells as determined by MTT assay. The curves in the figure are the repeated experiments results. Abscissa: concentration (ug/ml). Ordinate: % Cell Growth (determined by MTT assay). Figures 16C shows the growth curves of least sensitive: colon, cervix and lung cancer cells as determined by MTT assay. The curves in the figure are the repeated -10- WO 2006/029221 PCT/US2005/031900 experrmems "Tesmrs: • ADsuissa;-uuricentration (ug/ml). Ordinate: % Cell Growth (determined by MTT assay). Figure 17 shows the structure of Compound Y having the formula of C57H88O23 and the chemical name of 3-0-[p-D-galactopyranosyl(1-»2)]-a-L-arabinofuranosyl(1-»3)-p-D-5 glucuronopyranosyl-21,22-0-diangeloyl-3p, 15a, 16a, 21 p, 22a, 28-hexahydroxyolean-12-ene. Figure 18 shows the sprectrum of proton NMR of Compound Y. Figure 19 shows 2D NMR (HMQC) results of Compound Y. Figure 20 shows 2D NMR (HMBC) results of Compound Y. 10 Figure 21 shows the mass spectrum of compound Y with MALDI-TOF (high mass): Y + Matrix (CHCA) + Angiotensin 1 "two point calibration". Figure 22 shows the mass spectrum of compound Y with ESI-MS. Figure 23 shows the structure of Compound Y1 having the formula of C65H100O27 and the chemical name of 3-0-[p-D-galactopyranosyl(1->2)]-a-L-arabinofuranosyl(1^3)-p-15 D-glucuronopyranosyl-21-0-(3,4-diangeloyl)-a-L-rhamnophyranosyl-22-0-acetyl-3p,16a, 21 p, 22a, 28-pentahydroxyolean-12-ene. Figure 24 shows the Proton NMR spectrum of Compound Y1. Figure 25 shows the 2D NMR (HMQC) results of Compound Y1. Figure 26 shows the 2D NMR (HMBC) results of Compound Y1. 20 Figure 27 shows COSY-NMR profile of Compound Y1. Figure 28 shows the chemical structure and the chemical name of Compound Y2. Figure 29 shows the proton NMR spectrum of Y2. Figure 30 shows the 2D NMR spectrum of Y2 (HMQC)-leveM. Figure 31 shows the C13 NMR spectra of compound Y2. 25 Figure 32 shows the 2D NMR (HMBC)-level-l spectra of compound Y2. Figure 33 shows the 2D NMR HOHAHA (TOCSY)-level-l spectrum of compound Y2. Figure 34 shows the mass spectrum of compound Y2 +Matrix + Standards. Figure 35 shows the chemical structure of Y8. Figure 36 shows H-NMR spectrum of Y8. 30 Figure 37 shows C13-NMR spectrum of Y8. Figure 38 shows 2D NMR HMQC (level 1) spectrum of Y8. Figure 39 shows the chemical structure of Y9. Figure 40 shows H-NMR spectrum of Y9. Figure 41 shows 2D NMR HMQC (level 1) spectrum of Y9. -11 - WO 2006/029221 PCT/US2005/031900 Figlu'rfe'42 fehWS'^B'^NMRfllvrBC-flevell) spectrum of Y9. Figure 43 shows the chemical structure of Y10. Figure 44 shows H-NMR spectrum of Y10. Figure 45 shows C13 NMR spectrum of Y10. 5 Figure 46 shows 2D NMR HMQC (level 1) spectrum of Y10. Figure 47 shows the chemical structure and the chemical name of Compound R1. Figure 48 shows the Proton-NMR spectrum of compound R1. Figure 49 shows the 2D NMR (HMQC) spectrum of compound R1. Figure 50 shows the 2D NMR (HMBC) spectrum of compound R1. 10 Figure 51 shows the 2D NMR (COSY) spectrum of compound R1. Figure 52 shows the C13 NMR spectrum of compound R1. Figure 53 shows the chemical structure of Compound 054. Figure 54 shows the Proton-NMR spectra of compound 054. Figure 55 shows the 2D NMR (HMQC) spectra of compound 054. 15 Figure 56 shows the 2D NMR (HMBC) spectra of compound 054. Figure 57 shows the absorption spectrum of Xanthoceras sorbifolia extract of the invention. Abscissa: Wavelength in nm. Ordinate: Optical Density. The extract has three absorption maximum at 207nm, 278nm and 500nm. Figure 58 shows the proton NMR spectrum of Y4. 20 Figure 59 shows the 2D NMR (HMQC) spectrum of Y4. Figure 60 shows purification of component-R with HPLC. A: Extract from fraction #10 of FPLC (iso-30) was further separated by HPLC. B: Rechromatogram of the major component under same condition as described in A. Figure 61. Fractionation of Fraction-0 with HPLC with 20% acetonitrile isocratic elution 25 (iso-20). Figure 62 shows rechromatography of 054, 028 and 034 (from iso-20). Figure 63A shows the chemical structure of a compound, wherein R1 represents angeloyl group; R2 represents angeloyl group; R3 represents OH or H; R4 represents H or OH or CH3 or CH20R6 or COOR6; wherein R6=H or acetyl or sugar moiety; 30 Positions 23, 24, 25, 26, 27, 29, 30 of the compound independently comprise CH3 or CH20H or CHO or COOH or alkyls group or acetyl group or derivative thereof; R6 represents Ac or H and R5 represents sugar moiety, wherein the sugar moiety comprises at least one sugar, or D-glucose, or D-galactose, or L-rhamnose, or L-arabinose, or D-xylose, or alduronic acid, or D-glucuronic acid or D-galacturonic acid, or -12- WO 2006/029221 PCT/US2005/031900 derft/aitiv^ -fhfefedff SKthS-fediWih&tion thereof. In an embodiment, R5 represents a compound capable of performing the function of sugar moiety. Figure 63B shows the chemical structure of a compound wherein R1 represents angeloyl group; R2 represents angeloyl group; R3 represents Ac or H; R4 represents H 5 or OH; R6 represents Ac or H; R7 represents H or OH or CH3 or CH20R6 or COOR6 wherein R6=H or acetyl or sugar moiety; Positions 23, 24, 25, 26, 27, 29, 30 of the compound independently comprise CH3 or CH20H or alkyls group or acetyl group or derivative thereof and R5 represents sugar moiety, wherein the sugar moiety comprises at least one sugar, or D-glucose, or D-galactose, or L-rhamnose, or L-arabinose, or D-10 xylose, or alduronic acid, or D-glucuronic acid, or D-galacturonic acid, or derivative thereof, or the combination thereof. In an embodiment, R5 represents a compound capable of performing the function of the sugar moiety. Figure 64 shows HPLC (iso-45) profiles of FPLC fractions #5657. Figure 65 shows the effect of compound X on the growth of OCAR-3 cells. 15 Figure 66 shows H-NMR of compound X. Figure 67 shows 2D NMR (HMQC) of compound X. Figure 68 shows 2D NMR (HMBC) of compound X. Figure 69 shows C13-NMR of compound X. Figure 70 shows the chemical structure of compound X. 20 Figure 71 shows the mass spectrum 1 (WALDI-TOF) of compound X. Figure 72 shows the mass spectrum 2 (WALDI-TOF) of compound X. Figure 73 shows a comparision of MTT and Haemolytic activities of saponin compound and Compound Ys of the invention. (A) and (B) shows hemolytic activities. (C) and (D) show MTT activities. 25 Figure 74 (A) shows a compound of the invention without angeloyl groups. (B) shows a compound of the invention without sugar moiety. Figure 75 shows the saponin compounds of the invention. DETAILED DESCRIPTION OF THE INVENTION 30 This invention provides a compound selected from a compound of formula (1): -13- WO 2006/029221 PCT/US2005/031900 or a salt, ester or derivative thereof,wherein R1 represents angeloyl group; R2 represents angeloyl group; R3 represents OH or H; R4 represents CH3 or CH20H; R7 represents H; and R5 represents D-glucose or D-Galactose; and R6 represents L-5 arabinose. This invention provides a compound selected from a compound of formula (2): or a salt, ester or derivative thereof, wherein R1 represents angeloyl group; R2 10 represents angeloyl group; R3 represents Ac or H; R4 represents H or Ac; and R5 represents CH3 or CH2OH. This invention provides a compound selected from a compound of formula (3): 15 or a salt, ester or derivative thereof, wherein R1 represents angeloyl group; R2 represents angeloyl group; R3 represents OH or H; R4 represents CH3 or CH20H or -14- WO 2006/029221 PCT/US2005/031900 alkyisgroup ortneiraerivairvesrrco represents Ac or H and R5 represents sugar moiety, wherein the sugar moiety comprises at least one sugar, or D-glucose, or D-galactose, or L-rhamnose, or L-arabinose, or D-xylose, or alduronic acid, or D-glucuronic acid, or D-galacturonic acid, or derivative thereof, or the combination thereof. In an embodiment, 5 R5 represents a compound capable of performing the function of sugar moiety. In another embodiment the sugar moiety comprises L-arabinose, or D-glucose, or D-galactose, or combinations thereof. This invention provides a compound selected from a compound of formula (3A): or a salt, ester or derivative thereof, wherein R1 represents angeloyl group; R2 represents angeloyl group; R3 represents OH or H; Positions 23, 24, 25, 26, 27, 29, 30 of the compound independently comprise CH3, or CH20H, or CHO, or COOH, alkyls group, or acetyl group, or derivative; R6 represents Ac or H; and R5 represents sugar 15 moiety, wherein the sugar moiety comprises at least one sugar, or D-glucose, or D-galactose, or L-rhamnose, or L-arabinose, or D-xylose, or alduronic acid, or D-glucuronic acid, or D-galacturonic acid, or their derivative thereof, or the combination thereof. In an embodiment, R5 represents a compound capable of performing the function of the sugar moiety. In another embodiment the sugar moiety comprises L-20 arabinose, D-glucose and/or D-galactose, or combinations thereof. In a further embodiment, any two of R1, R2 or R6 are angeloyl groups, or any one of R1, R2 or R6 is attached to a sugar moiety in which two angeloyl groups are attached to adjacent carbons of the monosaccharides. In a further embodiment, R1, R2, and R6 comprises angeloyl group, acetyl group, tigloyl group, senecioly group, or an acid with two to five 25 carbons or combibation thereof. In a further embodiment, at least one of R1, R2 or R6 is attached a sugar moiety or rhamnose, wherein sugar moiety or rhamnose comprises two angeloyl group, acetyl group, tigloyl group, senecioly group, acid having two to five carbons, or combinations thereof. -15- WO 2006/029221 PCT/US2005/031900 This invention provides a uornpourfd selected from a compound of formula (4): 23 R7 (4) or a salt, ester or derivative thereof, HC •O hc ■0 wherein S1= 0R2 ; S2 = °Rl ;s3 = oh ; S4 = 0r2 oh ; 5 Wherein S1-S4 =S1 or S2 or S3 or S4; R1 represents angeloyl group; R2 represents angeloyl group; R3 represents Ac or H; R4 represents H or OH; R6 represents Ac or H; R7 represents CH3 or CH2OH or alkyl group or their derivatives and R5 represents sugar moiety or sugar chain selected from the group consisting of D-glucose, D-galactose, L-rhamnose, L-arabinose, D-xylose, alduronic acid, D-glucuronic acid, D-10 galacturonic acid and their derivatives. In an embodiment, R1 represents angeloyl group; R2 represents angeloyl group; R3 represents Ac or H; R4 represents H or OH; R6 represents Ac or H; Positions 23, 24, 25, 26, 27, 29, 30 of the compound independently comprise CH3, CH20H, CHO, COOH, alkyls group, acetyl group or derivative thereof; R5 represents sugar moiety, wherein the sugar moiety comprises at 15 least one sugar, or D-glucose, or D-galactose, or L-rhamnose, or L-arabinose, or D-xylose, or alduronic acid, or D-glucuronic acid or D-galacturonic acid, or their derivative thereof, or the combination thereof. In an embodiment, R5 represents a compound capable of performing the function of the sugar moiety. In another embodiment, R1, R2, R3, R6 comprises an angeloyl group, acetyl group, tigloyl group, senecioly group, or an 20 acid having two to five carbons. In a further embodiment, the sugar moiety comprises L-arabinose or/ and D-glucose or/and D-galactose, or combinations thereof. This invention provides a compound selected from a compound of formula (5): -16- WO 2006/029221 PCT/US2005/031900 Or a salt, ester, metabolite or derivative thereof, wherein R1 and R2 represent angeloyl group; R3 represents H or OH; R4 represent CH20R6; and wherein R6 is H; R5 represents at least one sugar moiety or its derivatives. In an embodiment, R1 and R2 5 represent angeloyl group; R3 represents H or OH; R4 represents COOR6 wherein R6 is H; R5 represents at least one sugar moiety or its derivatives. In an embodiment, R1 represents H; R2 represents angeloyl group; R3 represents H or OH; R4 represents CH20R6 or COOR6; wherein R6 is an angeloyl group; and R5 represents at least one sugar moiety or its derivatives. In another embodiment, at least two of R1, R2, and R6 10 comprise an angeloyl group or acid having five carbons; R3 represents H or OH; R4 represents CH20R6 or COOR6; and wherein R6 is angeloyl group; R5 represents at least one sugar moiety or its derivatives. In a further embodiment, at least one angeloyl of R1 or R2 is replaced by acetyl group, tigloyl group, senecioly group, or an acid having two to five carbons; R3 represents H or OH; R4 represents CH20R6 or COOR6; and 15 wherein R6 is angeloyl group; R5 represents at least one sugar moiety or its derivatives. In a further embodiment, at least one of R1, R2, and R6 is a sugar moiety or rhamnose comprising at least two angeloyl groups, acetyl group, tigloyl group, senecioly group, or an acid having two to five carbons or combination thereof. In a further embodiment, positions 23, 24, 25, 26, 29, 30 of the compound independently comprise CH3, CH20H, 20 CHO, COOH, alkyls group, acetyl group or derivative thereof. In a further embodiment, R5 represents sugar moiety comprising glucose, galactose or arabinose. In a further embodiment, R5 represents sugar moiety, wherein the sugar moiety comprises at least one sugar, or D-glucose, D-galactose, or L-rhamnose, or L-arabinose, or D-xylose, or alduronic acid, or D-glucuronic acid or D-galacturonic acid, or derivative thereof, or the 25 combination thereof. In an embodiment, R5 represents a compound capable of performing the function of the sugar moiety. In a further embodiment, the R5 represents H. In a further embodiment, R4 represents H or OH or CH3. Substitution, deletion and/or addition of any group in the above-described compounds 30 will be apparent to one of ordinary skill in the art based on the teaching of this -17- WO 2006/029221 PCT/US2005/031900 application: rrr rarcner-emDoaimeru, the substitution, deletion and/or addition of the group(s) in the compound of the invention does not substantially affect the biological function of the compound. In a further embodiment, the angeloyl groups are in a transposition on a structure. This invention provides a compound comprising the following structure: wherein R1 represents angeloyl group and R2 represents angeloyl group. In an embodiment, the biangeloyl group is acylated in trans-position. In another embodiment, the biangeloyl group is acylated in trans-position on adjacent carbons. In a further embodiment, the biangeloyl group is acylated in a structure. In a further embodiment, R1, R2 comprises angeloyl group, tigloyl group, senecioly group, an acid having two to five carbons, or combinations thereof. In a further embodiment, the compound comprises at least two angeloyl groups, tigloyl groups, senecioly groups, an acid having two to five carbons, or combinations thereof. In a further embodiment, the compound further comprises a sugar moiety. In a further embodiment, the sugar moiety comprises glucose, galactose or arabinose or combination thereof. In a further embodiment, the sugar moiety comprises at least one sugar, or D-glucose, or D-galactose, or L-rhamnose, or L-arabinose, or D-xylose, or alduronic acid, or D-glucuronic acid, or D-galacturonic acid, or their derivative thereof, or the combination thereof. In a further embodiment, the structure A, B or C comprises a compound capable of performing the function of the sugar moiety. Substitution, deletion and/or addition of any group in the above-described compounds will be apparent to one of ordinary skill in the art based on the teaching of this application. In a further embodiment, the substitution, deletion and/or addition of the group(s) in the compound of the invention does not substantially affect the biological function of the compound. In a further embodiment, the angeloyl groups are in a trans-position on a structure. A composition for treating cancers or inhibiting virus, comprising a compound which comprises at least two side chains which comprise angeloyl groups, wherein the side chains are at adjacent carbon in trans position. In an embodiment, the side chains are -18- WO 2006/029221 PCT/US2005/031900 at aitematrvexarDornn c/s positron. m an embodiment, the side chains are at alternative carbon in trans position. In an embodiment, the side chains are in non-adjacent carbon cis or trans position. In an embodiment, the side chains comprise a functional group capable of performing the function of angeloyl group. 5 This invention provides a composition for inhibiting tumor cell growth, comprising the above-described compounds. In an embodiment, the composition comprises a suitable carrier. In another embodiment, the composition comprises a pharmaceutical^ suitable carrier. This invention provides a method for treating ovarian cancer in a subject, 10 comprising administering to said subject an effective amount of the above-described compositions. This invention provides a method for isolating compounds from Xanthoceras sorbifolia herb, or plants from the sapindaceae family comprising the steps of: (a) extracting 15 Xanthoceras sorbifolia or plant powder with organic solvents to obtain an organic extract; (b) collecting the organic extract; (c) refluxing the organic extract to obtain a second extract; (d) removing the organic solvent from the second extract; (e) drying and sterilizing the second extract to obtain a crude extract powder; (f) fractionating the crude extract powder into components using HPLC and FPLC chromatography with silica gel, 20 C18 and other equivalent solid phase materials; (g) monitoring absorption wavelength at 207nm or 254nm; (h) identifying the bioactive components of the crude extract powder; (i) purifying one or more bioactive components of the crude extract powder with FPLC to obtain one or more fraction of the bioactive component; and (j) isolating the desired fraction of the bioactive component with preparative HPLC. 25 Compound Y or Y3 This invention provides a compound comprising the following structure, i.e., see Figure 17, having the formula of C57H88023 and the name of 3-0-[p-D-galactopyranosyl(1-»2)]-a-L-arabinofuranosyl(1 -»3)-(3-D-glucuronopyranosyl-21,22-0-diangeloyl-3p, 15a, 16a, 30 21 p, 22a, 28-hexahydroxyolean-12-ene, also known as Xanifolia-Y. This compound is obtainable from Xanthoceras sorbifolia or plants from the sapindaceae family. -19- WO 2006/029221 PCT/US2005/031900 ho oh This compound belongs to an oleanene triterpenoidal saponin with a trisaccharide chain attached at C-3 of the aglycone and two angeloyl groups acylated at C-21 and C-22. This compound has anti-cancer activity. The assignment of this structure is 5 supported by spectral data, i.e., H-NMR, 2D NMR (HMBC, HMQC), and MS (MALDl-TOF, EMS). Accordingly, this compound has the characteristic property as shown in Figures 18-22 or Table 5.1. Compound Y1 10 This invention provides another compound comprising the following structure, i.e., see Figure 23, having the formula of C65H100O27 and the name of 3-0-[(3-D-galactopyranosyl(1 ->2)]-a-L-arabinofuranosyl(1 -»3)-p-D-glucuronopyranosyl-21-0-(3,4-diangeloyl)-a-L-rhamnophyranosyl-22-0-acetyl-3p,16a, 21 p, 22a, 28-pentahydroxyolean-12-ene, also known as Xanifolia-Y1. This compound is a bisdesmosidic polyhydroxyoleanene triterpenoidal saponin with a trisaccharide chain at C-3 of the backbone and a monosaccharide moiety at C-21 where two angeloyl groups were acylated at C-3 and C-4 position. This compound has anti-cancer activity. The assignment of this structure is 20 supported by spectral data, i.e., H-NMR, 2D NMR (HMBC, HMQC, COSY), and MS (MALDl-TOF). Accordingly, this compound has the characteristic property as shown in Figures 24-27. 15 oh -20- WO 2006/029221 PCT/US2005/031900 Compound Y2 This invention provides a third compound comprising the following structure, i.e., see Figure 28, having the formula of C57H88O24 and chemical name of 3-O-03-D-5 glucopyranosyl-(1-»2)]-a-L-arabinofuranosyl(1 ->3)-p-D-glucuronopyranosyl-21,22-0-diangeloyl-3(3, 15a, 16a, 21(3, 22a, 24p, 28-heptahydroxyolean-12-ene, also known as Xanifolia-Y2. 10 This compound (Y2) belongs to saponins comprising a triterpene, a sugar moiety and angeloyl groups linked to the backbone. The angeloyl groups are linked to the backbone at C21 and C22 positions. This compound has anti-cancer activity. The assignment of this structure is supported by spectral data, i.e., H-NMR, C-NMR, 2D NMR (HMBC, HMQC, TOCSY), and MS (MALDl-TOF). Accordingly, this compound has the 15 characteristic property as shown in Figures 29-34. Compound Y8 This invention provides a fourth active compound Y8 and the structure was determined by 1D NMR, 2D NMR, and MS analysis. The compound comprises the following 20 structure, i.e. see Figure 35, having the formula of C57H87O23 and chemical name of 3-0-[/£-glucopyranosyl (1-»2)]-or-arabinofuranosyl (1-»3)-/?-glucuronopyranosyl-21, 22-0-diangeloyl-3/?, 16a, 21# 22a, 24/?, 28-hexahydroxyolean-12-ene, also known as Xanifolia-Y8. oh -21 WO 2006/029221 PCT/US2005/031900 ho oh The assignment of this structure is supported by spectral data, i.e., H-NMR, C13-NMR and 2D NMR (HMQC). Accordingly, this compound has the characteristic property as shown in figures 36-38. 5 Compound Y9 This invention provides a fifth active compound Y9 and the structure was determined by 1D NMR, 2D NMR, and MS analysis. The compound comprises the following structure, i.e., see Figure 39, having chemical name 3-0-[/?-galactopyranosyl (1->2)]-a-10 arabinofuranosyl (1 ->3)-/?-glucuronopyranosyl-21 -0-(3,4-diangeloyl)-a- rhamnopyranosyl-28-0-acetyl-3/?, 16a, 21/?, 22a, 28-pentahydroxyolean-12-ene, also known as Xanifolia-Y9. The assignment of this structure is supported by spectral data, i.e., H-NMR, 2D NMR 15 (HMQC and HMBC). Accordingly, this compound has the characteristic property as shown in Figures 40-42. Compound Y10 This invention provides a sixth active compound Y10 and the structure was determined 20 by 1D NMR, 2D NMR and MS analysis. The compound comprises the following structure, i.e., see Figure 43, having the formula of C57H87O22 and chemical name of 3-0-[/?-galactopyranosyl (1-»2)]-c*:-arabinofuranosyI (1->3)-/?-glucuronopyranosyl-21, 22- OH -22- WO 2006/029221 PCT/US2005/031900 10 15 20 Odiirtge1dyi-,3^*16t< "2T'PrTZa, 28-pentahydroxyolean-12-ene, also known as Xanifolia-Y10. The assignment of this structure is supported by spectral data, i.e., H-NMR, C13-NMR and 2D NMR (HMQC). Accordingly, this compound has the characteristic property as shown in figures 44-46. This invention provides a compound comprising a sugar moiety and a triterpene or Sapogenin, wherein the triterpene or sapogenin is acylated at Carbon 21 and 22 with angeloyl groups. In an embodiment, the triterpene or sapogenin is acylated at Carbon 21 and/or 22 with a sugar moiety which comprises two angeloyl groups. In another embodiment, the compound comprises one or more sugars. In another embodiment, the compound comprises at least two angeloyl groups. In a further embodiment, the sugar moiety comprises at least one sugar, or D-glucose, or D-galactose, or L-rhamnose, or L-arabinose, or D-xylose, or alduronic acid, or D-glucuronic acid or D-galacturonic acid, derivative thereof, or the combination thereof. In a further embodiment, a compound capable of performing the function of the sugar moiety is attached to the triterpene or sapogenin. In a further embodiment, the angeloyl group may be replaced by tigloyl group, senecioly group, an acid having two to five carbons, or combinations thereof. Substitution, deletion and/or addition of any group in the above-described compounds will be apparent to one of ordinary skill in the art based on the teaching of this application. In a further embodiment, the substitution, deletion and/or addition of the group(s) in the compound of the invention does not substantially affect the biological function of the compound. In a further embodiment, the angeloyl groups are in a transposition on a structure. In a further embodiment, the angeloyl groups are in an adjacent trans-position on a structure. Extracts obtained from Xanthoceras sorbifolia having anticancer activity is disclosed. Experiments for determining anti-cancer activity of the extract of the invention employ oh -23- WO 2006/029221 PCT/US2005/031900 humarrcelfs lihefe'tie'rrvecf flhorTi^lieVen human organs, i.e., (HTB-9 (bladder), HeLa-S3 (cervix), DU145 (prostate), H460 (lung), MCF-7 (breast), K562 (leukocytes), HCT116 (colon), HepG2 (liver), U20S (bone), T98G (brain) and OVCAR-3 (ovary)). The response of the 11 cell lines toward the extract of the invention can be categorized into 5 four groups: (A) most sensitive: Ovary, see Figure 14; (B) Sensitive: bladder, bone, (C) Semi-sensitive: prostate, leukocyte, liver, breast, and brain; and (D) lease sensitive: colon, cervix, and lung. See Figure 16A, B, C. The respective IC50 values are listed in Table 3.1. 10 Table 3.1. IC50 values of Xanthoceras Sorbifolia Extract Determined in Different Cancer Cells Cancer cells from different organs IC50 determined by MTT assay (ug/ml) Ovary (most sensitive) 15-15 Bladder (sensitive) 45-50 Bone 40-55 Prostate (semi-sensitive) 40-50 Leukocyte 45-50 Liver 45-65 Breast 65 Brain 70-85 Colon (least sensitive) 90 Cervix 115 Lung 110 In order to identify the active compounds of Xanthoceras sorbifolia, the extracts from Xanthoceras sorbifolia were separated by chromatography comprising FPLC (Fast 15 Protein Liquid Chromatography) and HPLC (High Preferment Liquid Chromatography). Multiple fractions were obtained by FPLC procedures, i.e., see Figure 9, and HPLC, i.e., see Figure 8. Analysis of the fractions by HPLC shows that the extract comprises 26 identifiable fractions, designated as a to z, which are shown in Figure 8. Anti-cancer activities of these fractions were determined by the MTT assay. 20 FPLC fraction 5962, i.e., see Figure 10, which coresponds to fraction Y in HPLC, i.e., see Figure 8, has anti-cancer activity. Compound Y can be purified from fraction Y. Fraction 5962 was further separated into 4 components, designated as Y1 to Y4. See -24- WO 2006/029221 PCT/US2005/031900 Figdrferi f. Cdrtipotlnds Yf-Y4::c&nbe purified from the fraction 5962. Fraction 6365 was further seperated into 5-6 components, designated as Y5-Y10. See Figure 12. Compounds Y5-Y10 can be purified from Fraction 6365. Compounds Y or Y3, Y1 and Y2 show strong anti-tumor activity, i.e., see Figure 2-3, and were therefore isolated and 5 purified. Similarly, compounds Y8, Y9 and Y10 also show strong anti-tumor activity, i.e., see Figure 4, and were therefore isolated and purified. See Figure 13. Accordingly, the structures of these active compounds, i.e., Y, Y1, Y2, Y8, Y9 and Y10 and their uses are the subject of this application. i 10 The inhibition effects of the compounds of the present invention on ovarian cancer cells were evaluated with the MTT assay. Compound Y shows at least 10 times higher potency (IC50 = 1.5 ug/ml), i.e., see Figure 2, than the original crude extract as shown in Figure 14 (IC50 = 20 ug/ml). The selectivity of compound Y toward different cell lines was tested, and it was found that compound Y has a much higher potency toward 15 ovarian cancer cells as compared to the cervical cancer cells. See Figure 15. This invention provides a method for identifying and isolating the bioactive compounds from plants, herbs or plant extracts. In an embodiement, the extracts include extracts of Xanthoceras sorbifolia or of plants from the sapindaceae family. This invention provides 20 the chemical structure of six bioactive compounds isolated from Xanthoceras sorbifolia or plants from the sapindaceae family. The structure of the compounds of the invention is shown in Figure 1. This invention provides spectral data including H-NMR, C-13-NMR, 2D NMR (HMBC, HMQC, COSY, TOCSY), and MS (MALDl-TOF, ESI-MS) in supporting the assigned structures. 25 This invention provides a consensus sub-structure or functional group derived from the bioactive compounds purified from fraction Y. The compounds, such as Y or Y3, Y1, Y2, Y8, Y9 and Y10, isolated from fraction Y are collectively referred to as "Ys" and their common name is Xanifolia-Ys. The consensus sub-structure or functional group of 30 these compounds is the biangeloyl group located on adjacent carbons. For example, in compound Y, Y2, Y8 and Y10, the biangeloyl group is located at 21 p and 22a of the triterpene backbone. See Figure 5. In compound Y1 and Y9, the biangeloyl group is located at C3 and C4 of the sugar ring. See Figure 6. Accordingly, the biangeloyl group of the bioactive compounds of the invention is situated in a trans-position with respect to 35 each other on a structure. See Figure 7. It has been shown that the bioactive functional -25- WO 2006/029221 PCT/US2005/031900 group't)f trie""CO'mpD'unas ,"OTJinfe invention is a biangeloyl group attached in-trans to adjacent carbons located in a structure. See Figure 7.This invention provides a salt of the above-described compounds. 5 This invention provides a composition comprising the above-described compounds and a suitable carrier. This invention provides a pharmaceutical composition comprising an effective amount of the above-described compounds and a pharmaceutically acceptable carrier. This invention provides an anti-ovarian cancer agent or composition comprising the above-described compositions. This invention provides a composition effective 10 against cancer growth. The cancer includes but is not limited to bladder cancer, bone cancer and ovary cancer. This invention provides a composition effective against skin cancer and KB cancer growth. This invention provides a composition comprising the above-described compounds and their salts, esters, derivatives or metabolites capable of inhibiting tumour growth. This invention provides a composition for inhibiting virus 15 growth and/or activities comprising the above-described compounds and their salts, esters, derivatives or metabolites. This invention provides a composition comprising the above-described compounds and their salts, esters, derivatives or metabolites capable of hemolytic activities. See Figure 20 73. It has been shown that a compound of the invention having two angeloyl groups has a stronger anticancer activity than a compound with one angeloyl group.See Figure 65, 2, 3. A compound with two angeloyl groups shows more haemolytic activity than a compound with one angeloyl group. See Figure 73 A. The compound loses hemolytic activity when the angeloyl groups are removed See Figure 73 B. The compounds of the 25 invention or their derivatives are also obtainable by chemical systhesis or isolated from natural or biological sources, including plants from the sapindaceae family. It has also been shown that saponin compounds of the invention having two angeloyl groups are more potent than Escin for treating diseases relating to hamoylic activities. See Figure 73A. 30 This invention provides a composition for treating chronic venous insufficiency, peripheral edema, antilipemic, chronic venous disease, varicose vein disease, varicose syndrome,venous stasis, Expectorant, cerebro-organic convulsion, cerebral circulation disorder, cerebral edema, psychoses, dysmenorrhea!, hemorrhoids, episiotomies, 35 haemonhoids, peripheral oedema formation or postoperative swelling; for reducing -26- WO 2006/029221 PCT/US2005/031900 symptoms orieg1 ■patri;" praritisnower leg volume, thrombosis, thromophlebitis; and for preventing gastric ulcers antispasmotic. This invention provides a composition for AntiMS, antianeurysm, antiasthmatic, antibradykinic, anticapillarihemorrhagic, anticephalagic, anticervicobrachialgic, antieclamptic, antiedemic, antiencaphalitic, 5 antiepiglottitic, antiexudative, antiflu, antifracture, antigingivitic, antihematomic, antiherpetic, antihistaminic, antihydrathritic, antimeningitic, antioxidant, antiperiodontic, antiphlebitic, antipleuritic, antiraucedo, antirhinitic, antitonsilitic, antiulcer, antivaricose, antivertiginous, cancerostatic, corticosterogenic, diuretic, fungicide, hemolytic, hyaluronidase inhibitor, lymphagogue, natriuretic, pesticide, pituitary stimulant, 10 thymolytic, vasoprotective, venotonic. Other compounds were also isolated from fraction R and fraction O of the extract of Xanthoceras sorbifolia, which are designated herein as R1 and 054, respectively. Their structures were determined. Both compounds are triterpenoidal saponins. Both 15 compounds lack biangeloyl acttachment in the triterpene backbone or in the sugar rings. Preliminary experiments indicate both R1 and 054 do not have anticancer activity. Compound R1 The structure of compound R1 is shown below and in Figure 47. Compound R1 has a 20 chemical formula of C65H106O29 and chemical name of 3-0-[angeloyl-(1-»3)-p-D-glucopyranosyl-(1->6)]-p-D-glucopyranosyl-28-0-[a-L-rhamnopyranosyl-(1->2)-p-D-glucopyranosyl-(1->-6)-p-D-glucopyranosyl-3p, 21 p, 22a, 28-tetrahydroxyolean-12-ene, also known as Xanifolia-R1. 25 The assignment of this structure is supported by spectral data, i.e., H-NMR, C-13-NMR, 2D NMR (HMBC, HMQC, COSY), and MS (MALDl-TOF, EMS). Accordingly, this compound has the characteristic property as shown in Figures 48-52. oh oh -27- WO 2006/029221 PCT/US2005/031900 uompouna-uo4 This invention provides a compound 054 isolated from the extract of Xanthoceras sorbifolia. The structure of 054 was determined and has a formula of C60H100O28. The Structure of Compound 054 is shown below.See also Figure 53. The chemical name of compound-054 is: 3-0-p-D-glucopyranosyl-(1-»6)]-p-D-glucopyranosyl-28-0-[a-L-rhamnopyranosyl-(1-»2)-p-D-glucopyranosyl-(1-»6)-p-D-glucopyranosyl-3p, 21 p, 22a, 28-tetrahydroxyolean-12-ene, also known as Xanifolia-054. The assignment of this structure is supported by spectral data, i.e., 1 H-NMR, 2D 10 NMR (HMBC, HMQC). Accordingly, this compound has the characteristic property as shown in Figures 54-56. In addition to the compound Ys, R1 and 054, other compounds were also isolated from fraction X of the extract of Xanthoceras sorbifolia, which are designated herein as X. Its 15 structure was determined. The compound is a triterpenoidal saponin with an angeloyl group attached at C22 of the triterpene. Compound-X This invention provides an active compound, designated as "compound X", isolated 20 from extract of Xanthoceras Sorbifolia. Compound X is an oleanene triterpenoidal saponin with a trisaccharide chain attached at C-3 of the aglycone and one angeloyl group acylated at C-22. The formula of compound X is C58H92O22, and the chemical name of compound X is: 3-0-{[/?-D-galactopyranosyl (1->2)]-[or-L-arabinofuranosyl (1-»3)]-/?-D-glucuronopyranoside butyl ester}-21-0-acetyl-22-0-angeloyl- 25 3/?,16a,21/?,22or,28-pentahydroxyolean-12-ene. The chemical structure of compound X is shown below. See also Figure 70. 5 oh oh -28- WO 2006/029221 PCT/US2005/031900 This invention provides a composition comprising the compounds of the invention for treating enuresis and frequency micturition, and for improving the functions of the 5 central nervous system including signaling the bladder to wake up from deep sleep or to relaxe the bladder so that it can store more urine. The compounds of the invention can be used to relax the detrusor tension caused by aging, stress, nervousness, overactivity, instability, hyper-reflexia, and uninhibited bladder. In another embodiment, the compounds may be used for relaxing the contracted bladder tissue induced by 10 acetylcholine (Ach). The compounds identified and isolated from extract of Xanthoceras sorbifolia may be used as acetylcolinesterase, an AChE inhibitor, for regulating Antidiuretic hormone (ADH), which reduces the volume of urine, and as an antiinflammatory agent. 15 The compounds of the invention can be used for accelerating the growth of bladder, for suppressing deep sleep, for increasing alterness in a sleeping subject, for modulating the release, breakdown and uptake of Antidieuretic hormone (ADH) and its receptors, for modulating the secretion, breakdown and uptake of Adrenocorticotropic hormone (ACTH) and its receptors, for modulating the release, breakdown and uptake of 5-20 hydroxytryptamine and its receptors, for modulating the release, breakdown and uptake of Acetycholine (Ach) and its receptors, for modulating the release, breakdown and uptake of Adrenaline (AD) and its receptors, for modulating the release, breakdown and uptake of Dopamine (DA) and its receptors, for modulating the release, breakdown and uptake of Norepinephrine (NE) and its receptors, for preventing sleep paralysis, for 25 modulating the formation, release, breakdown and activity of neuropeptides and their receptors. This invention provides a composition comprising the compounds of the invention for treating cancers; for inhibiting virus; for preventing cerebral aging; for improving -29- WO 2006/029221 PCT/US2005/031900 memory;"improviiig cerebral ranctrons, for curing enuresis, frequent micturition, urinary incontinence,dementia, Alzheimer's disease, autism, brain trauma, Parkinson's disease or other diseases caused by cerebral dysfunctions; for treating arthritis, rheumatism, poor circulation, arteriosclerosis, Raynaud's syndrome, angina pectoris, cardiac 5 disorder, coronary heart disease, headache, dizziness, kidney disorder; cerebrovascular diseasea; inhibiting NF-Kappa B activation; for treating brain edema, sever acute respiratory syndrome, respiratory viral diseases, chronic venous insufficiency, hypertension, chronic venous disease, anti-oedematous, anti inflammatory, haemonhoids, peripheral oedema formation, varicose vein disease, flu, post traumatic 10 edema and postoperative swelling; for inhibiting ethanol absorption; for lowering blood sugar; for regulating the adreocorticotropin and corticosterone level; and for treating impotence or premature ejaculation or diabetes. See U.S. Serial No. 10/906,303, filed February 14, 2005, International Application No. PCT/US04/43465, filed December 23, 2004, International Application No. PCT/US04/33359, filed October 8, 2004, and U.S. 15 Serial No. 11/131551, filed May 17, 2005, the contents of which are incorporated herein by reference. This invention provides a composition capable of regulating the enzymatic activities of bladder cell. The Xanthoceras Sorbifolia derived compounds and/or compositions of the invention are capable of regulating the various components or receptors of a cell and 20 strengthening the cell growth process. The Xanthoceras Sorbifolia derived compound and/or composition regulates the activities of cell's enzymes. See U.S. Serial No.60/675,284, filed April 27, 2005, the contents of which are incorporated herein by reference. This invention provides compounds isolated from Xanthoceras Sorbifolia, or its derivatives or metabolites capable of regulating pathways involved in cell 25 proliferation.See U.S. Serial No. 10/906,303, filed February 14, 2005, International Application No. PCT/US04/43465, filed December 23, 2004, International Application No. PCT/US04/33359, filed October 8, 2004, the contents of which are incorporated herein by reference. 30 This invention provides the methods and uses of triterpenoidal saponins purified and isolated from plants. This invention provides compositions comprising the triterpenoidal saponins or their derivatives for inhibition of tumor growth. The compounds of the invention comprise angeloyl group(s) or tigloyl group(s) or senecioyl group(s) or combinations thereof which are attached to carbon 21 and 22 of their sapongenines. In 35 an embodiment, the compounds may comprise any two angeloyl groups or tigeloyl -30- WO 2006/029221 PCT/VS2005/031900 groffp&' o'i" seWeclb^hgfoup'sr of "c'drnbinations thereof attached to a sugar moiety which bonds to carbon 21 or 22 of their sapongenines. The bioactive compounds can be isolated from natural plants, including plants in the Sapindaceae family, which has between about 1400-2000 species with 140-150 genera. See U.S. Serial No.60/675,282, 5 filed filed April 27, 2005. Summary This invention provides methods for identifying and purifying bioactive compounds from the plant extract of Xanthoceras sorbifolia. Eight bioactive compounds have so far been 10 identified and purified, and six of them have been shown to have anticancer activity. These compounds are collectively referred to as triterpenoidal saponins. A consensus sub-structure was identified or derived from the structure of the bioactive compounds of the invention. The consensus sub-structure or active functional groups of the bioactive compounds is the biangeloyl group located on adjacent carbons. The angeloyl groups 15 are located at 21 p and 22a of the triterpene backbone, i.e., see Figure 5, or located at C3 and C4 of the sugar ring, i.e., see Figure 6. Accordingly, the biangeloyl group of these bioactive compounds is acylated in a trans-position in adjacent carbons of a structure. See Figure 7. The compound with single angeloyl group shows weaker anticancer activity than a compound with two angeloyl groups, i.e., sees Figure 65, 2, 3. 20 The compound with single angeloyl group shows less haemolytic activity than a compound with two angeloyl groups, i.e., see Figure 73. The structures or derivatives of the compounds of the present invention are also obtainable by chemical systhesis or from biological sources. 25 This invention will be better understood from examples which follow. However, one skilled in the art will readily appreciate that the specific methods and results discussed are merely illustrative of the invention as described more fully in the claims which follow thereafter. 30 EXPERIMENTAL DETAILS Experiment 1: Herb Extraction (a) extracting Xanthoceras sorbifolia powder of husks or branches or stems or leaves or kernels or roots or barks with organic solvent at ratio of 1:2 for 4-5 times for 20-35 hours 35 each time to form an organic extract; (b) collecting the organic extract; (c) refluxing the -31 - WO 2006/029221 PCT/US2005/031900 orgdiniirektfaet Tor'2-3 times arstru to form second extract; (d) removing the organic solvent from the second extract; and (e) drying and sterilizing the second extract to form a Xanthoceras sorbifolia extract powder. 5 Experiment 2: Analysis of Xanthoceras Sorbifolia Extract Components by HPLC Chromatography Methods HPLC. A C-18 reverse phase |abondapak column (Water P/N 27324) was equilibrated 10 with 10% acetonitrile, 0.005% Trifluoroacetic acid (equilibration solution). An extract of Xanthoceras sorbifolia prepared using the methods described in Experiment 1 was dissolved in equilibration solution (1 mg/ml) before applying into the column. 20ug of samples was applied into column. Elution conditions: Fractions were eluted (with flow rate 0.5 ml/min.) with acetonitrile gradient from 10% to 80% in 70 min, and then remains 15 at 80% for 10 min. The acetonitrile concentration then decreased to 10% and remained at 10% for 25 min. The fractions were monitored at 207 nm and recorded in chart with a chart speed of 0.25 cm/min and with OD full scale of 0.128. Instruments. Waters Model 510 Solvent Delivery System; Waters 484 tunable Absorbance Detector; Waters 745/745B Data Module. 20 Absorbance analysis. The absorption profile of Xanthoceras Sorbifolia extract at various wavelengths was determined. An extract of Xanthoceras sorbifolia of the present invention was dissolved in 10% acetonitrile/TFA and scanned at 200-700 nm with a spectrophotometer [Spectronic Ins. Model Gene Sys2]. 25 Results HPLC. About 60-70 peaks can be accounted for in the profile. Among them four are major peaks, 10 are of medium size and the rest are small fractions. The peaks are labelled with a to z following increased concentration of acetonitrile elution. See Figure 8. 30 Absorption maximum. Three absorption maximum were identified for Xanthoceras sorbifolia plant extract; 207nm, 278nm and 500nm. See Figure 57. Experiment 3: Determination of the cell-growth activity effected by Xanthoceras Sorbifolia Extract with Cancer Cells Derived from Different Human Organs using 35 MTT Assay -32- WO 2006/029221 PCT/US2005/031900 Methods and Materials Cells. Human cancer cell lines were obtained from American Type Culture Collection: HTB-9 (bladder), HeLa-S3 (cervix), DU145 (prostate), H460 (lung), MCF-7 (breast), 5 K562 (leukocytes), HCT116 (colon), HepG2 (liver), U20S (bone), T98G (brain) and OVCAR-3 (ovary). Cells were grown in culture medium (Hel_a-S3, DU145, MCF-7, Hep-G2 and T98G in MEN (Earle's salts); HTB-9, H460, K562, OVCAR-3 in RPMI-1640; HCT-116, U20S in McCoy-5A) supplemented with 10% fetal calf serum, glutamine and antibiotics in a 5% C02 humidified incubator at 37°C. 10 MTT assay. The procedure for MTT assay followed the method described in (Carmichael et al., 1987) with only minor modifications. Cells were seeded into a 96-wells plate at concentrations of 10,000/well (HTB-9, HeLa, H460, HCT116, T98G, OVCAR-3), 15,000/well (DU145, MCF-7, HepG2, U20S), or 40,000/well (K562), for 24 hours before drug-treatment. Cells were then exposed to drugs for 48 hours (72 hours 15 for HepG2, U20S, and 96 hours for MCF-7). After the drug-treatment, MTT (0.5 mg/ml) was added to cultures for an hour. The formation of formazan (product of the reduction of tetrazolium by viable cells) was dissolved with DMSO and the O.D. at 490nm was measured by an ELISA reader [Dynatech. Model MR700]. The MTT level of cells before drug-treatment was also measured (TO). The % cell-growth (%G) is calculated 20 as: %G = (TD-T0 / TC-T0) x 100 (1) where TC or TD represent O.D. readings of control or drug-treated cells. When TO > 25 TD, then the cytotoxicity (LC) expressed as % of the control is calculated as: %LC = (TD-T0 / TO) x 100. (2) Results 30 Among the 11 cell lines studies, inhibition of cell-grwoth after exposure of plant extract was observed. However, their sensitivity toward Xanthoceras sorbifolia extract is different. The response of the cell lines to the Xanthoceras extract can be categorized into four groups: Most sensitive, i.e., Ovary; Sensitive, i.e., bladder, bone; Semi-sensitive, i.e., prostate, leukocyte, liver, breast, and brain; and least sensitive, i.e., -33- WO 2006/029221 PCT/US2005/031900 coldh^eWi^'atlWwhg. 'Sefe Figure 14, 15 and 16 A-D. Their IC50 values are listed in Table 3.1 above. In addition to cell-growth inhibition, the Xanthoceras sorbifolia plant extract also stimulate a minor cell growth at low concentrations in bladder, bone and lung cells. 5 Results indicate that there is a cell or tissue stimulation component(s) in the extract. See Figures 16A and 16D. To investigate the inhibition components of the Xanthoceras sorbifolia plant extract, the plant extract was fractionated. Figure 10 shows the results of the screening of fractions 10 obtained after FPLC chromatography for cell growth-inhibition activity. The assay was conducted with bladder cells. The fractions obtained from FPLC, as shown in Figure 9, were used. As shoton in Figure 9, different components of Xanthoceras sorbifolia extract caused either growth or inhibition effects on cells. Only fractions 5962, designated as Fraction Y, cause cell growth inhibition. Abscissa: concentration (ug/ml). Ordinate: % 15 Cell Growth (determined by MTT assay). Experiment 4: Purification of the Inhibition Components in the Xanthoceras Sorbifolia Extract. 20 (A) Fractionation of plant extracts with FPLC Methods Column. Octadecyl functionalized silica gel. Column dimension: 2cm x 28cm; equilibrated with 10% acetonitrile - 0.005% TFA before use. Sample loading: 1-2 ml, concentration: 100mg/ml in 10% acetonitrile/TFA. 25 Gradient elution condition: 10-80% acetonitrile in a total volume of 500 ml. Monitor absorption wavelength: at 254nm. Fraction Collector: 5 ml/fractions (collect from 10% to 72% acetonitrile) Instrument: AKTA-FPLC, P920 pump; Monitor UPC-900; Frac-900. 30 Results The elution profile of the chromatography shows 4-5 broad fractions. See Figure 9. These fractions were analyzed with HPLC. Specific components, corresponding to a-z as specified in Figure 8, are then assigned in these FPLC fractions. FPLC fractions are then grouped into 7 pools and analyzed for cell growth activity in bladder cells with MTT 35 assay. See Experiment 3. It was found that only pool #5962, corresponding to fraction Y -34- WO 2006/029221 PCT/US2005/031900 in HPtC, "actrvity. See Figure 10. It was also found in later experiments that fractions beyond 62 also show inhibition activity. The components isolated from fractions 63-65 showed inhibition activities. See Figure 4,12 and 13. 5 (B) Isolation of Component Ys with Preparative HPLC Methods Column: A preparative HPLC column (Waters Delta Pak C18-300A); Elution conditions: 45% acetonitrile isocratic elution with flow rate of 1 ml/min. Fractions are monitored at 207nm and were collected and lyophilized. 10 Results Final separation of Y fractions was achieved by HPLC with a preparative column. See Figure 11 and 12. These fractions, which include compound Y1, Y2, Y or Y3 and Y4, were collected. Re-chromatography of compound Y showed a single peak in HPLC with 15 a C18 reverse phase column. See Figure 11A and 11B. Re-chromatography of the compound Y8, Y9 and Y10 showed a single peak in HPLC with a C18 reverse phase column. See Figure 13. (C) Appearance and solubility 20 The pure compound Ys is an amorphous white powder, soluble in aqueous alcohol, i.e., methanol or ethanol, 50% acetonitrile and 100% pyridine. (D) Inhibition analysis of Compound Ys with MTT assay Inhibition analysis of compound Y was determined with MTT assay. Figure 2 shows that 25 compound Y has activity against ovarian cancer cells (OCAR-3) with IC50 value of 1.5 ug/ml which is 10-15 times more potent than the unpurified extract shown in Figure 14. Figure 15 shows the selectivity of compound Y to ovarian cancer cells compared with ' cervical cancer cells (HeLa). Figure 3 shows the inhibition activities of compound Y1 and Y2 on the growth of ovarian cancer cells (OCAR-3). Figure 4 shows the inhibition 30 activities of compound Y, Y8, Y9 and Y10 on the growth of ovarian cancer cells (OCAR-3). Experiment 5: Determination of the Chemical Structure Methods -35- WO 2006/029221 PCT/US2005/031900 NMR "^nfotysTsnTie"' purencompouna Y of Xanthoceras sorbifolia was dissolved in pyridine-D5 with 0.05% v/v TMS. All NMR spectra were acquired using a Bruker Avance 600 MHz NMR spectrometer with a QXI probe (1H/13C/15N/31P) at 298 K. The numbers of scans for 1D 1H spectra were 16 to 128, depending on the sample 5 concentration. 2D HMQC spectra were recorded with spectral widths of 6000 x 24,000 Hz and data points of 2024 x 256 for t2 and t1 dimensions, respectively. The number of scans was 4 to 128. 2D HMBC were acquired with spectral widths of 6000 x 30,000 Hz and data points of 2024 x 512 for t2 and t1 dimensions, respectively. The number of scans was 64. The 2D data were zero-filled in t1 dimension to double the data points, 10 multiplied by cosine-square-bell window functions in both t1 and t2 dimensions, and Fourier-transformed using software XWIN-NMR. The final real matrix sizes of these 2D spectra are 2048x256 and 2048x512 data points (F2xF1) for HMQC and HMBC, respectively. Mass spectral analysis. The mass of samples was analyzed by (A) MALDl-TOF Mass 15 Spectrometry and by (B) ESI-MS Mass spectrometry. (A) Samples for MALDl-TOF were first dissolved in acetonitrile, and then mixed with the matrix CHCA, i.e., Alpha-cyano-4-hydroxycinnamic acid, 10mg CHCA/mL in 50:50 water/acetonitrile and 0.1% TFA in final concentration. The molecular weight was determined by the high resolution mass spectroscope analysis with standards. (B) For ESI, the sample was analyzed with LCQ 20 DECA XP Plus machine made by Thermo Finnigan. It is ionized with ESI source and the solvent for the compound is acetonitrile. Results The profile of the proton NMR is presented in Figure 18. The 2D NMR profiles of HMQC 25 and HMBC are shown in Figures 19 and 20, respectively. Table 5.1 summarizes the 2D NMR chemical shift data and the assignment of functional groups derived from these data. Based on these data and analysis, the structure of compound Y (Y3) is assigned as shown below. 30 Structure of Compound Y (See also Figure 17) -36- WO 2006/029221 PCT/US2005/031900 The chemical name of compound Y is: 3-0-[p-D-galactopyranosyl(1->2)]-a-L-arabinofuranosyl(1->3)-(3-D-glucuronopyranosyl-21,22-0-diangeloyl-3p, 15a, 16a, 21 p, 22a, 28-hexahydroxyolean-12-ene. The mass spectrum of compound Y as determined 5 by MALDl-TOF and ESI-MS, i.e., see Figure 21, 22, indicates that the mass of compound Y is 1140.57 which agree with the theoretical mass of the compound Y. Conclusion The active compound Y isolated from extract of Xanthoceras sorbifolia is an oleanene 10 triterpenoidal saponin with a trisaccharide chain attached at C-3 of the aglycone and two angeloyl groups acylated at C-21 and C-22. The formula of Y is C57H88O23, and the chemical name of Compound Y is: 3-0-[p-D-galactopyranosyl(1-»2)]-a-L-arabinofuranosyl(1-»3)-p-D-glucuronopyranosyl-21,22-0-diangeloyl-3p, 15a, 16a, 21 p, 22a, 28-hexahydroxyolean-12-ene. 15 Experiment 6: Determination of the Chemical Structure of Compound Y1 of Xanthoceras Sorbifolia Extract Methods 20 The method for NMR and MS analysis for compound Y1 is similar to the method described in Experiment 5. Results The spectrum of the H-NMR is presented in Figure 24. The 2D NMR spectra of HMQC, 25 HMBC and COSY are shown in Figures 25, 26 and 27, respectively. Table 6.1 summarizes the chemical shift data and the assignment of functional groups derived from these data. Based on these data and analysis, the structure of compound Y1 is assigned and shown below. Structure of Y1 (See also Figure 23) -37- WO 2006/029221 PCT/US2005/031900 The chemical name of Y1 is: 3-0-[p-D-galactopyranosyl(1-*2)]-a-L-arabinofuranosyl(1-»3)-p-D-glucuronopyranosyl-21-0-(3,4-diangeloyl)-a-L-rhamnophyranosyl-22-0-acetyl-3p,16a, 21 p, 22a, 28-pentahydroxyolean-12-ene. 5 Conclusion Compound Y1 isolated from extract of Xanthoceras sorbifolia is a bisdesmosidic polyhydroxyoleanene triterpenoidal saponin with a trisaccharide chain at C-3 of the backbone and a monosaccharide moiety at C-21 where two angeloyl groups were 10 acylated at C-3 and C-4 position. The formula of Y1 is C65H100O27. Experiment 7: Determination of the Chemical Structure of Compound Y2 of Xanthoceras Sorbifolia Extract. 15 Methods The method for NMR and MS analysis for compound Y2 is similar to the method described in Experiment 5. Results 20 The 1D and 2 D NMR spectra of H-NMR, C-13 NMR, HMQC, HMBC and (TOCSY) and MS (MALDl-TOF) of Y2 are showed in Figures 29-34. Table 7.1 summarizes the 1D and 2D NMR chemical shift data and the assignment of functional groups derived from these data. 25 Conclusion Based on these data and analysis, the compound Y2 isolated from extract of Xanthoceras sorbifolia is an oleanene triterpenoidal saponin with a trisaccharide chain attached at C-3 of the aglycone and two angeloyl groups acylated at C-21 and C-22. The chemical structure of Y2 is shown below. See also Figure 28. -38- WO 2006/029221 PCT/US2005/031900 HC HO- OH The formula of Y2 is C57H88O24, and the chemical name of Compound Y2 is: 3-0-[p-D-glucopyranosyl-(1 -»2)]-a-L-arabinofuranosyl(1 ->3)-p-D-glucuronopyranosyl-21,22-0-diangeloyl-3p, 15a, 16a, 21 p, 22a, 24p, 28-heptahydroxyolean-12-ene. 5 Experiment 7B. Chemical structure analysis of Y4 Results of Y4 analysis The profile of the proton NMR of Y4 is presented in Figure 58. The profiles of 2D NMR 10 (HMQC) of Y4 is presented in Figure 59. Experiment 8. Purification of the Inhibition Components Y8-Y10 in the Xanthoceras Sorbifolia Extract 15 (A) Fractionation of Xanthoceras Sorbifolia extracts components with FPLC Methods The methods for this experiment are similar to the methods decribed in Experiment 4 Section (A) and (B). 20 Results The elution profile shows 4-5 broad fractions. See Figure 9. These fractions were analyzed with HPLC. FPLC fractions 63, 64 and 65 are further separated on 45% isocratic analysis, 4-5 major components were separated. See Figure 12. These fractions were assigned designations Y8, Y9 and Y10. These fractions were collected. 25 Re-chromatography of the compound Y8, Y9 and Y10 showed a single peak in HPLC with a C18 reverse phase column. See Figure 13. (B) Inhibition analysis with MTT assay. -39- WO 2006/029221 PCT/US2005/031900 Inhibition'' anarysisrorpurmea compounds was determined with the MTT assay. Results indicate that compound Y8, Y9 and Y10 has activity against ovarian cancer cells (OCAR-3) with IC50 values of 3, 4 and 1.5 ug/ml, respectively. See Figure 4. 5 Experiment 9. Determination of the Chemical Structure of Compound Y8 of Xanthoceras Sorbifolia Extract The method for NMR and MS analysis for compound Y8 is similar to the method 10 described in Experiment 5. The spectral profiles of the H-NMR, C13-NMR and 2D NMR (HMQC) of compound Y8 are presented in Figures 36-38. Table 9.1 summarizes the 1D and 2D NMR chemical 15 shift data and the assignment of functional groups derived from these data. Based on these data and analysis, the compound Y8 isolated from extract of Xanthoceras sorbifolia is an oleanene triterpenoidal saponin with a trisaccharide chain attached at C-3 of the aglycone and two angeloyl groups acylated at C-21 and C-22. The formula of compound Y8 is C57H87O23, and the chemical name of Y8 is: 3-0-[/?-glucopyranosyl 20 (1-»2)]-a-arabinofuranosyl (1-»3)-/?-glucuronopyranosyl-21, 22-0-diangeloyl-3/?, 16a, 21/?, 22a, 24/?, 28-hexahydroxyolean-12-ene. The chemical structure of compound Y8 is presented in the following figure. See also Figure 35. 25 Experiment 10. Determination of the Chemical Structure of Compound Y9 of Xanthoceras Sorbifolia Extract Methods Results OH Methods -40- WO 2006/029221 PCT/US2005/031900 TheHftetfto'd "W NR/fR"' afleF rsts* analysis for compound Y9 is similar to the method described in Experiment 5. Results 5 The spectral profiles of the H-NMR and 2D NMR, i.e., HMQC and HMBC, of Y9 are shown in Figures 40-42. Table 10.1 summarizes the 1D and 2D NMR chemical shift data and the assignment of functional groups derived from these data. Based on these data and analysis, compound Y9 isolated from extract of Xanthoceras sorbifolia is a bisdesmosidic polyhydroxyoleanene triterpenoidal saponin with a trisaccharide chain at 10 C-3 of the backbone and a monosaccharide moiety at C-21 where two angeloyl groups were acylated at C-3 and C-4 position. The formula of compound Y9 is C65Hioo027and the chemical name of Y9 is: 3-0-[/?-galactopyranosyl (1->2)]-a-arabinofuranosyl (1-»3)-/%glucuronopyranosyl-21-0-(3,4-diangeloyl)-a-rhamnopyranosyl-28-0-acetyl-3/?, 16a, 21/7, 22a, 28-pentahydroxyolean-12-ene. The chemical structure of Compound Y9 is 15 presented in the following figure. See also Figure 39. Experiment 11. Determination of the Chemical Structure of Compound Y10 of Xanthoceras Sorbifolia Extract 20 Methods The method for NMR and MS analysis for compound Y10 is similar to the method described in Experiment 5. 25 Results The profile of the H-NMR, C13-NMR and 2D NMR (HMQC) are shown in Figures 44-46. Table 11.1 summarizes the 1D and 2D NMR chemical shift data and the assignment of functional groups derived from these data. Based on these data and analysis, compound Y10 isolated from extract of Xanthoceras sorbifolia is an oleanene 'O oh 41 WO 2006/029221 PCT/US2005/031900 triierpenoiaai "sapontn wiirra Linsciuuiiaride chain attached at C-3 of the aglycone and two angeloyl groups acylated at C-21 and C-22. The formula of compound Y10 is C57H87O22, and the chemical name of Y10 is: 3-0-[/?-galactopyranosyl (1->2)]-a-arabinofuranosyl (1-»3)-/?-glucuronopyranosyl-21, 22-0-diangeloyl-3/7, 16a, 21/?, 22a, 5 28-pentahydroxyolean-12-ene. The chemical structure of Compound Y10 is presented in the following figure. See also Figure 43. Experiment 12. Purification of Component R from Xanthoceras Sorbifolia Extract 10 (A) Purification of Xanthoceras Sorbifolia extracts components with FPLC and HPLC The methods used are similar to the methods described in Experiment 4, section (A) and (B) except a 30% acetonitrile isocratic elution was used in HPLC for isolation of the 15 Compound R. Fraction No. 39-41 from gradient elution of FPLC were pooled and further purified with an open ODS-C18 column with isocratic 30% acetonitrile elution. Six identifiable 20 fractions in two groups were collected. Fractions 6-13 were further characterized with HPLC. These fractions were further separated into 4-5 components with the 30% acetonitrile isocratic elution in a DeltaPak column. The fraction designated herein as "R1", is the major component. See Figure 60A. The pure R1 was subsequently collected from the column elution. See Figure 60B. 25 (B) Appearance and solubility The pure R1 appears as an amorphous white powder, soluble in aqueous alcohol, i.e., methanol orethanol, 50% acetonitrile and 100% pyridine. oh Methods Results -42- WO 2006/029221 PCT/US2005/031900 (C) Ddtertriirtatrott ot the' cnemicai structure of R1 Methods The NMR and MS Analysis of R1 is similar to the method described in Experiment 5. 5 Results The NMR spectra of pure R1 are presented in Figures 48-52. Based on chemical shift analysis, compound R1 isolated from extract of Xanthoceras sorbifolia is a triterpenoid saponins with five sugars and one angeloyl group attached to the sugar moiety. The The formula of Compound R1 is C65H106O29, and the chemical name of R1 is: 3-0-[angeloyl-(1->3)-p-D-glucopyranosyl-(1->6)]-p-D-glucopyranosyl-28-0-[a-L-rhamnopyranosyl-(1 -»2)-p-D-glucopyranosyl-(1 ->6)-p-D-glucopyranosyl-3p, 21 p, 22a, 28-tetrahydroxyolean-12-ene. 15 Experiment 13: Purification of Component-0 from Xanthoceras Sorbifolia extract (A) Fractionation of Xanthoceras Sorbifolia extracts components with FPLC and HPLC 20 Methods The methods used are similar to the methods described in Experiment 4, section (A) and (B) except a 20% acetonitrile isocratic elution was used in HPLC for isolation of the Compound O. 25 Results Fractions obtained from FPLC were analyzed with HPLC. By comparison with the profiles of the original sample, a specific component, in this case fraction O, was identified (#28-30). Fraction O was collected for further purification. Sixteen identifiable -43- WO 2006/029221 PCT/US2005/031900 HPl!O%frsPctiohfe=lM,^0'bSfe^sawlie elution profiles. See Figure 61. Fractions 28, 34 and 54 were further purified. See Figures 62. These purified components are named as compound 028, 034 and 054, respectively. 5 (B) Appearance and solubility The purified compound 023 and 034 are light yellow amorphous powder, soluble in aqueous alcohol, i.e., methanol, ethanol, 50% acetonitrile and 100% pyridine. The purified compound 054 is a white amorphous powder, soluble in aqueous alcohol, i.e., methanol, ethanol, 50% acetonitrile and 100% pyridine. (C ) Structure analysis of Compound 054 Methods The NMR and MS analysis of 054 is similar to the method described in Experiment 5. 15 Results The NMR spectra of compound 054 is presented in Figures 54-56. Based on the chemical shift analysis, compound 054 isolated from extract of Xanthoceras sorbifolia is a bisdesmosidic polyhydroxyoleanene triterpenoidal glycoside with a disaccharide chain [/?-D-glucopyranosyl-(1->6)-/£-D-glucopyranoside] affixed to C-3 and a trisaccharide 20 chain [a-L-rhamnopyranosyl-(1-»2)-/?-D-glucopyranosyl-(1-»6)-y&-D-glucopyranosyl ester] attached to C-28. The chemical structure of compound 054 is presented in the following figure. See also Figure 53. The formula of compound 054 is C60H100O28, and the chemical name of 054 is: 3-0-p-25 D-glucopyranosyl-(1~>6)]-(3-D-glucopyranosyl-28-0-[a-L-rhamnopyranosyl-(1 -»2)-p-D-glucopyranosyl-(1-»6)-p-D-glucopyranosyl-3p, 21 p, 22a, 28-tetrahydroxyolean-12-ene. 10 oh oh -44- WO 2006/029221 PCT/US2005/031900 Experiment T4rFurmcationoruomponent-X from Xanthoceras Sorbifolia extract (A) Fractionation of Xanthoceras Sorbifolia extracts components with FPLC and HPLC 5 Methods The methods used are are similar to the methods described in Experiment 4, section (A) and (B) except collect FPLC fractions 56 and 57 which contain compound X were further separated with preparative HPLC. 10 Results Five major and seven minor peaks were observed in the chromatogram. Compound X was eluted near the middle of the elution profile (Figure 64). Compound X was collected and lyophilized. 15 (B) Appearance and solubility The purified Compound X is white powder and is soluble in 50% acetonitrile. (C) Inhibition analysis of Compound X with MTT assay 20 MTT analysis of compound X in OVCAR-3 cells indicates some inhibition activity. The IC50 of compound X is approximately 27 ug/ml. See Figure 65. Experiment 15. Determination of the Chemical Structure of Compound X of Xanthoceras Sorbifolia Extract 25 Methods The method for NMR and MS analysis for compound X is similar to the method described in Experiment 5. 30 Results The profile of the proton and C13 NMR are presented in Figure 66 and 69, respectively. The 2D NMR profiles of HMQC and HMBC are shown in Figure 67 and 68. Table 15.1 summarizes the 2D NMR chemical shift data and the assignment of functional groups derived from these data. Based on these data and analysis, the structure of compound 35 X is assigned as shown below. The mass spectrum of Compound X as determined by -45- WO 2006/029221 PCT/US2005/031900 MArot-TOF'tRQ^te^l 'irfclT^lriclicates the mass of compound Y is 1140.60 which agree with the theoretical mass of the compound X. Conclusion The active compound X isolated from extract of Xanthoceras Sorbifolia is an oleanene triterpenoidal saponin with a trisaccharide chain attached at C-3 of the aglycone and one angeloyl group acylated at C-22. The formula of X is C58H92O22, and the chemical name of Compound X is: 3-0-{[/?-D-galactopyranosyl (1-»2)]-|>-L-arabinofuranosyl (1 -»3)]-/?-D-glucuronopyranoside butyl ester}-21 -O-acetyl-22-O-angeloyl- 3/?,16a,21/?,22a,28-pentahydroxyolean-12-ene. The chemical structure of compound X is presented in the following figure. See also Figure 70. Experiment 16. Determination the haemolytic activities of Compound Y of Xanthoceras Sorbifolia Methods: • Human whole blood was obtained from Houston Gulf Coast Blood Center • Red blood cells were isolated by the following method: Human blood (in EDTA) was diluted 1:1 with PBS, underlay with 4 ml of Histopaque-1077 (SIGMA) and was centrifuged at 400g for 30 min. • Red blood cells (RBC) were collected and washed three times with PBS. • 10% suspension of RBC were prepared with PBS before use. • 50 ul of RBC suspension was added to 2 ml of Saponins with different concentration. • The suspension was mixed by vortexing and sit at room temperature for 60 OH minutes -46- WO 2006/029221 PCT/US2005/031900 THe' Stfsp^risiOft Was centrrruged at 3000 rpm for 5 min. Absorbance of the supernatant was measured at 540 nm. Results: In this experiment, hemolytic activity of human red blood cells by Xanifolia-Y (#63Y), 5 Escin and SIGMA saponin standard were compared. Y contains two angeloyl groups, Escin has one angeloyl group and SIGMA saponin standard is a mixture of saponins from Quillaia bark. The results show that #63Y (compound Y) has higher hemolytic activity (IC50=1 ug/ml) than Escin or SIGMA saponin standard (IC50=5 ug/ml). See Figure 73 A. 0 Experiment 17. Determination the hemolytic and MTT activities of Compound Y after removal of the angeloly or the sugar moiety by alkaline or acid hydrolysis, respectively 15 Methods: (A) Alkaline Hydrolysis of Xanifolia-Y. 20 mg of Xanifolia-Y was dissolved in 0.5 ml of 1M NaOH. The solution was incubated in 80C water bath for 4 hours. It was cooled to room temperature before neutralized with 0.5 ml 1 N HCI (adjust pH to about 3). The mixture was extracted with 2 ml 1-butanol 3 times. The butanol fractions were collected 20 and lyophilized. The hydrolyzed saponin with further purified with HPLC in a C-18 column eluted with 25% acetonitrile. (B) Acid Hydrolysis of Xanifolia-Y. 15 mg Xanifolia-Y was dissolved in 1 ml of Methanol. 1 ml of 2N HCI was then added. The mixture was refluxed in 80C water bath for 5 hours. The solution was then neutralized by adding 2 ml of 1N NaOH (to final pH 3-4). The aglycone was then extracted with ethylacetate 3 ml x 25 3. The extracts were collected and pooled. Further isolation of aglycone (sugar-removed Xanifolia-Y) was achieved by HPLC with isocratic elution of 80% acetonitrile. Results: The angeloly groups or the sugar moiety of the compound Y, were removed by alkaline or acid hydrolysis, respectively. The hemolytic activities of the hydrolysed products 30 were then analyzed. Resultsof these studies indicate that removing sugars reduce hemolytic activity, but removing angeloyl groups destroy the hemolytic activity. It also suggested that sugars are helpful but not essential for hemolytic activity. See Figure 73 B. The experiment results show that compound-Y lost MTT activities if the angeloyl groups were removed. However, the MTT activities were very weak when the sugar 35 moiety of the compound was removed. See Figure 73 C, 73 D. Results of comparison of -47- WO 2006/029221 PCT/US2005/031900 herffofitid actiVitM^* fl'etwgeh Cotfrpound Y, Escin and Saponin standard from SIGMA are shown in Figure 73 A and 73 B. Results of Comparison of hemolytic activities between Compound Y, compound Y without sugar moiety or angeloly groups are shown in Figure 73 B. Chemical structures of compound Y without sugar moiety or angeloly 5 groups are shown in Figure 74 A and 74B respectively. Experiment 18. Determination the anti virus activities of Compound Y The major procedures for the determination of antivirus activity of Compound Y are: A. Determine the production of HIV virus after a non-lethal dosage of Compound Y is 10 added to the viral culture system. B. Determine the growth activity of HIV virus after contact to compound Y. The steps for these experiments are: 1. Pre-treat HIV virus with different dosages of Compound Y for variable length of time. 2. Remove compound Y from virus. 15 3. Mix treated virus with cells. 4. Measure Virus production. 5. Negative control:-no virus in cell. 6. Positive control - untreated virus mixed with cell. Result: The virus growth will be inhibited after treatments of Compound Y. TABLES OF EXPERIMENTAL RESULTS: Table 5.1.13C and 1H NMR Data for Compound Y (in Pyridine-d5)a Position C H Key HMBC correlations 1 38.7 0.83, 1.40 C-3, C-5, C-9 2 26.4 1.81, 2.14 - 3 89.6 3.25, 1H, dd, 12.0/4.0 Hz C-23, C-24, GIcA C-1' 4 39.4 — - 5 55.3 0.78 — 6 18.5 1.55, 1.59 C-8, C-10 7 36.5 2.00, 2.10 C-5, C-9 8 41.2 — — 9 47.0 3.06 C-7, C-8, C-12, C-14, C-26 10 37.2 — — 11 23.7 1.74, 1.89 — 12 125.2 5.49,1H, br s C-9, C-11, C-14, C-18 13 143.4 — — 14 47.5 _ — 15 67.3 4.21 C-8, C-27 16 73.6 4.45 C-14, C-15, C-18 17 48.3 — — 18 40.8 3.07 C-12, C-13, C-14, C-16, C-19, C-20, C-28, -48- WO 2006/029221 PCT/US2005/031900 ^ L, it U "5i» *Ui ^ .41, 1.69 - 20 36.2 - 21 79.3 6.71 1H, d, 10 Hz C-20, C-22, C-29, C-30, 21-O-Ang C-1"" 22 73.5 6.32, 1H, d, 10 Hz C-16, C-17, C-21, C-28, 22-0-Ang C-1"" 23 27.7 1.26, 3H, s C-3, C-4, C-5, C-24 24 16.5 1.16, 3H, s C-3, C-4, C-5, C-23 25 16.0 0.81, 3H, s C-1, C-5, C-9, C-10 26 17.3 0.99, 3H, s C-7, C-8, C-9, C-14 27 21.0 1.85, 3H, s C-8, C-13, C-14, C-15 28 62.9 3.50, 1H, d, 11.0 Hz, 3.76, 1H, d, 11.0 Hz, C-16, C-17, C-18, C-22 29 29.2 1.09, 3H, s C-19, C-20, C-21, C-30 30 20.0 1.32, 3H, s C-19, C-20, C-21, C-29 GIcA r 104.9 4.89, 1H,d, 7.8 Hz C-3 2' 79.1 4.38 GIcA C-1', C-3', Gal C-1" 3' 86.1 4.20 GIcA C-2', C-4', Ara C-1'" 4' 71.5 4.42 GIcA C-3', C-5', C-6' 5' 78.0 4.52 GIcA C-4', C-6' 6' 171.9 — Gal 1" 104.6 5.32, 1H, d, 7.7 Hz GIcA C-2' 2" 73.6 4.42 Gal C-1", C-3" 3" 74.9 4.10 Gal C-2" 4" 69.5 4.56 Gal C-2", C-3" 5" 76.4 3.94 Gal C-4", C-6" 6" 61.6 4.43, 4.52 Gal C-4", C-5" Ara-f -j in 110.6 6.03. 1H, brs GIcA C-3', Ara C-2'", C-4m 2>n 83.4 4.94 Ara C-3'" 3", 78.3 4.78 Ara C-2"' 4,M 85.2 4.82 Ara C-5'" 5'" 62.2 4.12, 4.28 Ara C-3'" 21-O-Ang -| llll 167.7 - — 2,fM 129.6 — — 3"" 137.2 5.96, 1H, dq, 7.0/1.5 Hz Ang C-1"", C-4"", C-5"" 4,im 15.5 2.10, 3H, dq, 7.0/1.5 Hz Ang C-2"", C-3"" 5"" 20.8 2.00, 3H, s Anq C-1"", C-2"", C-3"" 22-0-Ang ^ III! 167.9 — - 2"" 129.8 - - 3"„ 136.3 5.78, 1H, dq, 7.0/1.5 Hz Ang C-1"", C-4"", C-5"" 4.M1 15.5 1.93, 3H, dq, 7.0/1.5 Hz Ang C-2"", C-3"" -49- WO 2006/029221 PCT/US2005/031900 5-' irt 1 T.74, 3H, s Ang C-1"", C-2"", C-3" The data were assigned based on HMQC and HMBC correlations. Table 6.1.13C and 1H NMR Data for Compound Y1 (in Pyridine-d5) Position H 1 38.6 0.85, 1.33 2 26.3 1.86, 2.10 3 89.7 3.25 (1H, m) 4 39.5 5 55.5 0.75 6 18.3 1.40, 1.43 7 33.1 1.20, 1.50 8 40.0 9 46.7 1.69 10 36.5 11 23.5 1.75, 1.91 12 123.6 5.37 (1H, brs) 13 143.0 — 14 41.8 — 15 34.7 1.53, 1.73 16 68.5 4.45 17 48.2 — 18 39.9 3.04 19 47.6 1.30, 3.05 20 36.7 — 21 85.3 5.05 (1H, d, J = 9.6 Hz) 22 73.8 6.17 (1H, d, J = 9.6 Hz) 23 27.7 1.29 (3H, s) 24 16.5 1.16 (3H, s) 25 15.5 0.78 (3H, s) 26 17.1 0.82 (3H, s) 27 27.3 1.83 (3H, s) 28 63.7 3.42, 3.60 (each, 1H, d, J =10.6 Hz) 29 29.9 1.42 (3H, s) 30 19.9 1.37 (3H, s) 3-O-GlcA-p 1 105.5 4.93 (1H, d, J =7.8 Hz) 2 78.6 4.37 3 86.0 4.20 4 71.6 4.43 5 78.0 4.50 6 171.8 — Gal-p 1 104.5 5.33 (1H, d, J =7.8 Hz) 2 73.5 4.43 3 74.9 4.10 4 69.5 4.57 5 76.3 3.95 6 61.1 4.44, 4.53 -50- WO 2006/029221 PCT/US2005/031900 F'AralK'"" * ~ ™" 1 110.9 6.04 (1H, brs) 2 83.3 4.95 3 78.3 4.78 4 85.2 4.82 5 62.0 4.13,4.31 21-0-Rham-p 1 105.1 4.88 (1H, d, J= 1.5 Hz) 2 70.5 4.25 3 74.0 5.59 4 71.5 5.70 5 68.5 3.89 6 17.6 1.18 (3H, d, J = 6.6 Hz) Rham-3-Ang 1 167.3a 2 128.2" 3 138.5° 5.98f (1H, q, J = 7.2 Hz) 4 15.7d 2.02s' (3H, d, J = 7.2 Hz) 5 20.6s 1.92" (3H, s) Rham-4-Ang 1 167.2a 2 128.0" 3 138.2° 5.88^(1 H, q, J = 7.2 Hz) 4 15.5d 1.969 (3H, d, J = 7.2 Hz) 5 20.5e 1.85" (3H, s) 22-O-Acetyl 1 171.4 2 21^8 2.31 (3H, s) a"n The data with the same labels in each column may be interchangeable. Table 7.1: 13C and 1H NMR data for Y2 (in Pyridine-d5)a Position C H 1 38.4 0.83, 1.36 2 26.4 1.89, 2.25 3 91.3 3.39, 1H, m 4 43.4 — 5 56.7 0.87, 1H, d, 12.0 Hz 6 18.6 1.31, 1.57 7 36.3 1.97, 2.12 8 40.7 — 9 46.7 1.63 10 36.6 — 11 23.9 1.69, 1.89 12 125.1 5.48, 1H, br s 13 143.4 - 14 47.5 — 15 67.1 4.18, 1H, d, 4.1 Hz 16 73.2 4.43 17 48.1 - -51 - WO 2006/029221 PCT/US2005/031900 18 „;S.„ 19 46.6 1.40, 3.08 20 36.1 — 21 78.3 6.69, 1H, d, 10.2 Hz 22 73.1 6.30, 1H, d, 10.2 Hz 23 22.0 1.29, 3H, s 24 62.9 3.28,1 H, d, 11.2 Hz; 4.32 25 15.6 0.64, 3H, s 26 17.1 0.94, 3H, s 27 20.8 1.84, 3H, s 28 63.1 3.48, 3.72 (each, 1H, d, 10.6 Hz) 29 29.3 1.09, 3H, s 30 20.0 1.32, 3H, s 3-O-GlcA 1 104.5 4.87, 1H, d, 7.2 Hz 2 78.6 4.31 3 86.5 4.23 4 71.6 4.45 5 77.4 4.53 6 171.9 Glc 1 103.7 5.48, 1H, d, 7.8 Hz 2 75.3 4.02 3 78.0 4.31 4 69.3 4.52 5 78.2 3.62 6 61.5 4.33, 4.50 Ara 1 110.1 6.05, 1H, brs 2 83.5 4.97 3 77.8 4.74 4 85.0 4.84 5 62.2 4.18,4.33 21-O-ang 1 167.5 — 2 128.7 — 3 137.2 5.95, 1H, dd, 14.4/7.2 Hz 4 16.7 2.08, 3H, d, 7.2 Hz 5 20.6 2.00, 3H, s 22-O-ang 1 167.9 — 2 128.9 3 136.3 5.76, 1H, dd, 14.4/7.2 Hz 4 15.6 1.95, 3H, dd, 7.2 Hz 5 20.4 1.74, 3H, s The data were assigned based on COSY, HMQC and HMBC correlations Table 9.1.13C and 1H NMR Data for Y8 (in pyridine-cfe) -52- WO 2006/029221 PCT/US2005/031900 PBsitidn f ,3 ,Ji„ "3# !iJi «Ji 1j_| 1 38.2 0.74, 1.30 2 26.3 1.85, 2.26(1 H, m) 3 91.1 3.30 (1H, m) 4 43.4 5 55.9 0.82 6 18.2 1.22, 1.48 7 32.9 1.24, 1.49 8 39.8 — 9 46.5 1.67 10 36.2 — 11 23.8 1.70, 1.83 12 123.3 5.39 (1H, brs) 13 142.5 — 14 41.4 — 15 34.6 1.64, 1.83 16 68.4 4.53 17 47.8 — 18 39.7 3.09 19 47.0 1.39, 3.11 20 36.2 21 78.5 6.68 (1H, d, J= 10.2 Hz) 22 73.4 6.30 (1H, d, J =10.2 Hz) 23 22.1 1.32 (3H, s) 24 63.2 3.28, 4.31 (each, 1H, d, J= 10.8 Hz) 25 15.4 0.62 (3H, s) 26 16.4 0.78 (3H, s) 27 27.3 1.82 (3H, s) 28 63.3 3.39, 3.62 (each, 1H, d, J =10.8 Hz) 29 29.3 1.08 (3H, s) 30 20.0 1.32 (3H, s) 3-O-Glc A-p 1 104.5 4.93 (1H, d, J = 7.2 Hz) 2 78.0 4.23 3 86.2 4.25 4 71.6 4.44 5 77.3 4.53 6 171.9 — Glc-p 1 103.7 5.48 (1H, d, J =7.2 Hz) 2 75.3 4.04 3 77.8 4.27 4 69.3 4.48 5 78.2 3.61 6 61.1 4.38, 4.48 Ara-f 1 111.1 6.04 (1H, brs) 2 83.5 4.97 3 77.4 4.84 -53- WO 2006/029221 PCT/US2005/031900 —l I ".»!! "it >Ui It jl It 11 7"T— 5 62.1 4.12, 4.37 21-O-Ang 1 167.5 — 2 128.9 3 137.0 5.93 (1H, q, J = 7.2 Hz) 4 15.7 2.07 (3H, d, J = 7.2 Hz) 5 20.8 2.00 (3H, s) 22-O-Ang 1 167.9 — 2 128.9 3 136.2 5.87 (1H, q, J =7.2 Hz) 4 15.6 2.03 (3H, d, J =7.2 Hz) 5 20.6 1.88 (3H,s) a"9 The data with the same labels in each column may be interchangeable. Table 10.1 13C and 1H NMR Data for Y9 (in pyridine-c/5) Position 13c qH 1 38.5 0.83, 1.36 2 26.3 1.80, 2.08 (1H,m) 3 89.5 3.26(1 H, m) 4 39.5 — 5 55.6 0.71 6 18.4 1.23, 1.46 7 32.8 1.23, 1.52 8 40.0 — 9 46.7 1.67 10 36.5 — 11 23.7 1.77, 1.88 12 123.5 5.41 (1H, br s) 13 142.8 - 14 41.7 — 15 34.5 1.56, 1.88 16 67.8 4.81 17 46.6 — 18 40.2 2.80 (1H, m) 19 47.5 1.36, 3.10 (1H, m) 20 36.7 — 21 91.8 4.83 22 71.3 4.37 23 27.7 1.26 (3H, s) 24 16.5 1.13 (3H,s) 25 15.5 0.79 (3H, s) 26 16.9 0.95 (3H, s) 27 27.3 1.82 (3H, s) 28 65.9 4.22, 4.33 (each, 1H, d, J= 10.2 Hz) 29 29.9 1.49 (3H, s) 30 20.0 1.33 (3H, s) -54- WO 2006/029221 PCT/US2005/031900 1 105.9 4.93 (1H, d, J = 7.2 Hz) 2 78.5 4.36 3 86.1 4.20 4 71.6 4.40 5 77.6 4.51 6 171.9 Gal-p 1 104.5 5.31 (1H, d, J = 7.6 Hz) 2 73.5 4.42 3 74.9 4.09 4 69.5 4.57 5 76.3 3.95 6 61.6 4.40,4.54 Ara-f 1 111.0 6.03 (1H, brs) 2 83.3 4.93 3 78.0 4.76 4 85.2 4.81 5 62.1 4.12, 4.29 21-0-Rham-p 1 105.1 4.87 (1H, d, J= 1.5 Hz) 2 70.5 4.39 3 74.0 5.58 4 71.1 5.70 5 69.0 3.89 6 17.0 1.11 (3H, d, J = 6.6 Hz) Rham-3-O-Ang 1 167.6a 2 128.36 3 138.6° 5.93f (1H, q, J = 7.2 Hz) 4 15.7^ 1.95 (3H,m) 5 20.7e 1.94s (3H,s) Rham-4-O-Ang 1 167.5a 2 128.0" 3 138.5° 5.87f (1H, q, J = 7.2 Hz) 4 15.6d 1.95 (3H,m) 5 20.6e 1.859(3H, s) 28-O-Acetyl 1 170.1 2 20.5e 1.849 (3H, s) a'9 The data with the same labels in each column may be interchangeable. Table 11.1 13C and 1H NMR Data for Y10 (in pyridine-cfe) Position 13C nH 1 38.5 0.87,1.38 2 26.4 1.86,2.12 (1H, m) 3 89/7 3.24 (1H, dd, J = 12.0/4.2 Hz) -55- WO 2006/029221 PCT/US2005/031900 —PglVXi ir trit ii ft 5 55.6 0.75 6 18.2 1.29, 1.49 7 32.9 1.27, 1.54 8 39.8 — 9 46.7 1.68 10 36.5 — 11 23.6 1.70, 1.83 12 123.3 5.40 (1H, br s) 13 142.5 — 14 41.4 — 15 34.8 1.60, 1.83 16 68.4 4.49 17 47.8 — 18 39.7 3.06 19 47.0 1.40, 3.10 20 36.1 — 21 78.5 6.69 (1H, d, J= 10.2 Hz) 22 73.5 6.31 (1H, d, J =10.2 Hz) 23 27.7 1.30 (3H, s) 24 16.5 1.17 (3H,s) 25 15.4 0.80 (3H, s) 26 16.7 0.83 (3H, s) 27 27.3 1.83 (3H, s) 28 63.4 3.40, 3.64 (each, 1H, d, J = 10.8 Hz) 29 29.3 1.09 (3H, s) 30 20.1 1.33 (3H, s) 3-O-Glc A-p 1 104.9 4.91 (1H, d, J = 7.8 Hz) 2 78.7 4.40 3 86.1 4.23 4 71.5 4.44 5 77.1 4.53 6 171.8 — Gla-p 1 104.6 5.34 (1H, d, J = 7.8 Hz) 2 73.4 4.50 3 74.9 4.11 4 69.6 4.58 5 76.4 3.98 6 61.6 4.47, 4.52 Ara-f 1 110.9 6.05 (1H, br s) 2 83.4 4.95 3 77.5 4.78 4 85.2 4.83 5 62.1 4.16, 4.39 21-O-Ang 1. 167.5 — 2 128.8 — -56- WO 2006/029221 PCT/US2005/031900 p£-r "" f37.# •* 5.92 (1H, q, 7.2 Hz) 4 15.7 2.07 (3H, d, 7.2 Hz) 5 20.8 2.00 (3H, s) 22-O-Ang 1 167.9 2 128.8 3 136.8 5.87 (1H, q, 7.2 Hz) 4 15.6 2.03 (3H, d, 7.2 Hz) 5 206 1.88 (3H, s) Table 15.1.13C and 1H NMR data for Compound-X (in MeOH-d4) Position C H 1 38.5 1.00, 1.64 2 25.7 1.70, 1.85 3 90.9 3.18 (1H, dd, J = 11.4/3.6 Hz) 4 39.1 — 5 55.6 0.78 (1H, d, J= 11.4 Hz) 6 17.9 1.44, 1.58 7 33.5 1.40, 1.69 8 39.8 — 9 46.4 1.67 10 36.4 — 11 23.3 1.89, 1.93 12 123.9 5.38 (1H, brs) 13 143.7 — 14 40.9 — 15 32.5 1.38, 1.60 16 68.8 3.99 17 48.5 — 18 39.6 2.64 (1H, m) 19 46.6 1.20, 2.68(1 H, m) 20 35.8 — 21 80.6 6.00 (1H, d, J = 10.2 Hz) 22 72.4 5.88 (1H, 6, J= 10.2 Hz) 23 27.0 1.08 (3H, s) 24 15.0 0.98 (3H, s) 25 15.4 0.88 (3H, s) 26 15.9 0.94 (3H, s) 27 26.3 1.49 (3H, s) 28 63.0 2.91, 3.25 (each, 1H, d, J = 11.4 Hz) 29 28.4 0.90 (3H, s) 30 18.8 1.11 (3H, s) 3-O-GlcA-p 1 104.1 4.55 (1H, d, J= 7.8 Hz) 2 77.3 3.74 3 85.0 3.68 4 70.7 3.62 5 75.0 3.86 6 170.3 — butyl-1 59.8 3.02 (2H, m) -57- WO 2006/029221 PCT/US2005/031900 DutyI-2 - «- PhtU2h^|£jgJL ma 1.05 butyl-3 18.4 1.51 butyl-4 12.5 1.16 3-O-Gal-p 1 103.1 4.65 (1H, d, J = 7.8 Hz) 2 72.0 3.54 3 73.6 3.50 4 69.1 3.82 5 75.8 3.46 6 61.3 3.59, 3.70 Ara-f 1 109.4 5.24 (1H, d, J= 1.8 Hz) 2 82.0 4.10 3 76.4 3.87 4 83.9 4.06 5 61.5 3.59, 3.70 21-O-Acetyl 1 169.9 — 2 21.6 2.14 (3H, s) 22-O-Ang 1 167.8 — 2 127.5 — 3 140.0 6.17 (1H, q, J = 7.2 Hz) 4 14.7 2.02 (3H, d, J = 7.2 Hz) 5 19.6 1.85 (3H, s) Although the present invention has been described in detail with particular reference to preferred embodiments thereof, it should be understood that the invention is capable of other different embodiments, and its details are capable of modifications in various 5 obvious aspects. As is readily apparent to those skilled in the art, variations and modifications can be affected while remaining within the spirit and scope of the invention. Accordingly, the foregoing disclosure, description, and figures are for illustrative purpose only, and do not in any way limit the invention which is defined only by the claims. 10 REFERENCES 1. Carmichael, J., DeGraff, W.G., Gazdar, A.F., Minna, J.D. and Mitchell, J.B.: Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47:936-942 (1987). 15 2. Chen, Q. 1995. Methods of study on pharmacology of Chinese medicines. Press of People's Public Health, Beijing. p892. -58- WO 2006/029221 PCT/US2005/031900 3. "'HUang, ZtiPSftrpLiuf M.T'lfehen, Ch. Zh. 1997. Study on effects of Yangshou Dan on improving learning and retention. Chinese Journal of combination of Chinese and west medicine, 9(17): 553. 4. Zhang, Y., Zhang, H. Y., Li, W. P. 1995. Study on effects of Anjifu on improving 5 intelligence, Chinese Bulletin of Pharmacology, 11 (3): 233. 5. Yang, J., Wang, J., Feng, P. A. 2000. Study on effects of Naokkangtai capsule on improving learning and retention in mice, New Chinese Medicine and Clinical Pharmacology, 1(11): 29. 6. Yang, J., Wang, J., Zhang, J. Ch. 2000. Study on effects of Crude saponins of 10 peonies on improving learning and retention in mice, Chinese journal of Pharmacology, 2(16): 46. 7. Xia, W. J., Jin, M. W., Zhang, L. 2000. Study on treatment of senile dementia caused by angio-aging with Didang tang, Pharmacology and Clinical of Chinese Medicines, 16 (4). 15 8. Bian, H. M., Yu, J. Z., Gong, J. N. 2000. Study on effects of Tongmai Yizhi capsule on improving learning and retention in mice, Pharmacology and Clinical of Chinese Medicines, 16 (5): 40. 9. Wei, X. L., Zhang, Y. X. 2000. Study of animal model for studying senile dementia, Chinese journal of Pharmacology, 8(16): 372. 20 10. Bureau of Medicinal Police, Department of Public Health. Guide line for study of effect of medicines for treatment of nervous system diseases, in Guidebook of study of new medicine. p45. 11. Zhang, D. Sh., Zhang, J. T. 2000. Effects of crude Ginseng saponins on improving impairment induced by B- peptide, Chinese journal of Pharmacology, 8(16): 22. -59- RECEIVED at IPONZ on 29 Mar 2011 </div>

Claims

<div class="application article clearfix printTableText" id="claims"> What is claimed is:

1. Use of a compound for manufacturing of a medicament for inhibiting cancer cell growth, wherein the cancer is ovarian cancer, wherein the compound has the following 5 general formula (5): or a salt or ester thereof, wherein R1 is selected from H, angeloyl group, acetyl group, tigloyl group, senecioyi group, a sugar moiety and a rhamnose; wherein the sugar moiety or rhamnose has at least two groups select from the group consisting of 10 angeloyl group, acetyl group, tigloyl group, and senecioyi group,; wherein R2 is selected from H, angeloyl group, acetyl group, tigloyl group, senecioyi group , a sugar moiety and a rhamnose; wherein the sugar moiety or rhamnose has at least two groups select from the group consisting of angeloyl group, acetyl group, tigloyl group, and senecioyi group; wherein R3 is H or OH; wherein R4 is CH20R6 or COOH 15 wherein R6 is H or angeloyl group, acetyl group, tigloyl group, or senecioyi group; wherein at least two of R1, R2 and R6 are selected from acetyl group, tigloyl group, senecioyi group, and a sugar moiety or a rhamnose; wherein the sugar moiety or rhamnose is substituted with at least two groups selected from the group consisting of angeloyl group, acetyl group, tigloyl group, senecioyi group; wherein each of positions 20 23, 24, 25, 26, 29, 30 of the compound is independently selected from CH3, CH20H, CHO, COOH, alkyls group, and acetyl group; wherein R5 is H or at least one sugar moiety(ies); wherein the sugar moiety(ies) is/are selected from glucose, galactose, rhamnose, arabinose, xylose, alduronic acid, glucuronic acid and galacturonic acid; wherein the number of sugar moieties of R5 can not be 4 when R1 is angeloyl and R2 25 is senecioyi group; wherein the compound can not be 3-0-[/?-D- galactopyranosyl(1^2)]-a-L-arabinofuranosyl(1^-3)-/?-D-glucuronopyranosyl-21,22-0- diangeloyl-3/?, 15a, 16a, 21/?, 22a, 28-hexahydroxyolean-12-ene, or 3-0-[p-D- galactopyranosyl(W2)]-a-L-arabinofuranosyl(1^3)-p-D-glucuronopyranosyl-21-0- (3,4-diangeloyl)-a-L-rhamnophyranosyl-22-0-acetyl-3p,16a, 21 p, 22a, 28- 30 pentahydroxyolean-12-ene; wherein postion 24 can not be CH3 when each of R1 and R2 is independently selected from Angeloyl, acetyl and sugar moiety with angeloyl, -60- RECEIVED at IPONZ on 29 Mar 2011 wherein the sugar moiety with angeloyl is L-rhamnopyranosyl substituted with Angeloyl at the 0-3 and 0-4 positions .

2.The use of claim 1, wherein one of R1, R2 , R6 is an angeloyl group, and at least one 5 of rest two of R1, R2 , R6 is independently selected from angeloyl group, tigloyl group, senecioyi group; or wherein at least one of R1, R2 , R6 comprises a sugar moiety or a rhamnose, wherein the sugar moiety or rhamnose has at least two groups select from the group consisting of angeloyl group, tigloyl group, senecioyi group; or wherein at least two of R1, R2 , R6 are independently attached with an angeloyl group; wherein 10 postion 24 is CH20H.

3. A compound having the following formula (1), (2), or (3): or a salt or ester thereof, wherein each of R1 and R2 is independently selected from 15 Angeloyl, acetyl and sugar moiety with angeloyl, wherein the sugar moiety with angeloyl is substituted with Angeloyl at the 0-3 and 0-4 positions; R3 comprises OH or H; R4 comprises CH3 or CH20H; R7 comprises H or Ac; R5 comprises glucose or Galactose; and R6 comprises arabinose; wherein R4 can not be CH3 when R5 is galactose or or a salt or ester thereof, wherein R1 comprises angeloyl group; R2 comprises angeloyl group; R3 comprises Ac or H; R4 comprises Ac or H; and R5 comprises CH2OH; 61 RECEIVED at IPONZ on 29 Mar 2011 or: or a salt, ester thereof, wherein Ri comprises angeloyl group; R2 comprises angeloyl group; R3 comprises OH or H; R4 comprises CH3 or CH2OH; R6 comprises Ac or H; and 5 R5 comprises 3 moieties; wherein the moieties are selected from glucose, galactose, arabinose, alduronic acid including glucuronic acid and galacturonic acid, and ester including butyl ester; wherein R4 can not be CH3 when R5 are glucuronic acid, galactose and arabinose unless R6 is Ac. 10

4. The compound of claim 3, having one of the following structures and formulae: (a) A compound having structure (Y2): or chemical name: 3-0-[p-D-glucopyranosyl-(1^-2)]-a-L-arabinofuranosyl(W3)-p-D-glucuronopyranosyl-21,22-0-diangeloyl-3p, 15a, 16a, 21 p, 22a, 24p, 28-heptahydroxyolean-12-ene, or Xanifolia-Y2; 20 ( b) A compound having structure (Y8): 62 RECEIVED at IPONZ on 29 Mar 2011 ^=\ Y-8 ^=\ o o Cl°°h o- (Y8) OH or chemical name: 3-0-[/?-glucopyranosyl (1^2)]-a-arabinofuranosyl (1^-3 )-/?-glucuronopyranosyl-21, 22-0-diangeloyl-3/?, 16a, 21/?, 22a, 24/?, 28-hexahydroxyolean-12-ene, or Xanifolia-Y8; 5 ( c) A compound having structure (Y9): (Y9), or chemical name: 3-0-[/?-galactopyranosyl (1^-2)]-a-arabinofuranosyl (1^-3)-/?-glucuronopyranosyl-21-0-(3,4-diangeloyl)-a-rhamnopyranosyl-28-0-acetyl-3/?, 16a, 21/?, 10 22a, 28-pentahydroxyolean-12-ene, or Xanifolia-Y9; ( d) A compound having structure (Y10): (Y10), or chemical name: 3-0-[/?-galactopyranosyl (W2)]-a-arabinofuranosyl (1^-3)-/?-15 glucuronopyranosyl-21, 22-0-diangeloyl-3/?, 16a, 21/?, 22a, 28-pentahydroxyolean-12-ene, or Xanifolia-Y10; 'O (e) A compound having structure of compound X: 63 RECEIVED at IPONZ on 29 Mar 2011 or chemical name: 3-0-{[/?-D-galactopyranosyl (W2)]-[a-L-arabinofuranosyl (1^-3)]-/3-D-glucuronopyranoside butyl ester}-21-0-acetyl-22-0-angeloyl-3/?,16a,21/?,22a,28-pentahydroxyolean-12-ene. 5

5. The compound of claim 3, wherein the compound comprises a triterpene backbone, said triterpene backbone is acylated at either 21 p or 22a position or at both 21 p and 22a position with an angeloyl group or a sugar moiety, wherein at least one sugar in the sugar moiety is substituted with angeloyl groups at the C3 and C4 position of said sugar; 10 wherein the triterpene backbone comprise 3 sugar moieties selected from glucose, galactose, rhamnose, arabinose, , and alduronic acid including glucuronic acid and galacturonic acid, and ester thereof.

6. The compound of any of claims 3 to 5, wherein the compound is in the form of a 15 salt.

7. The compound of any of claims 3 to 5, wherein the structure is synthesized or isolated from natural sources. 20

8. The compound of any one of claims 3 to 5 suitable for use as a medicament or health food.

9. Use of a composition comprising an effective amount of the compound of any of claims 3 to 5 for manufacturing of a medicament for inhibiting tumor or cancer cell 25 growth. 64 RECEIVED at IPONZ on 29 Mar 2011

10. The use of claim 9, wherein the cancer is breast cancer, leukocyte cancer, liver cancer, ovarian cancer, bladder cancer, prostate cancer, skin cancer, bone cancer or brain cancer. 5

11. Use of the compound of any of claims 3 to 7 for manufacturing of a medicament for inhibiting cancers; for inhibiting virus, HIV, fungi or bacteria; for preventing cerebral aging; for improving memory; improving cerebral functions, for curing enuresis, frequent micturition, urinary incontinence,dementia, Alzheimer's disease, autism, brain trauma, Parkinson's disease or other diseases caused by cerebral dysfunctions; for treating 10 arthritis, rheumatism, poor circulation, arteriosclerosis, Raynaud's syndrome, angina pectoris, cardiac disorder, coronary heart disease, headache, dizziness, kidney disorder; cerebrovascular diseasea; for inhibiting NF-Kappa B activation;for treating brain edema, sever acute respiratory syndrome, respiratory viral diseases, chronic venous insufficiency, hypertension, chronic venous disease, anti-oedematous, anti inflammatory, 15 haemonhoids, peripheral oedema formation, varicose vein disease, flu, post traumatic edema and postoperative swelling; for reducing symptoms of pain; for inhibiting ethanol absorption, reducing the formation of blood clots, lower blood sugar; for regulating adreocorticotropin and corticosterone levelregulating the release of ACTH, PGF2, antagonism to 5-HT or histamine; for reducing catabolism of tissue 20 mucopolysaccharides; for treating impotence, premature ejaculation, or diabetes.

12. A compound for inhibiting cancers or inhibiting virus selected from any of claims 3 to 5, wherein the compound is sapongenin or triterpene having at least two adjacent angeloyl groups or wherein the sapongenin or triterpene is aclyated with 2 acids, 25 wherein the acids have two to five carbons.

13. The use of claim 1, wherein the compound has at least two angeloyl groups or substituted two acids in side chains and trans to one another; or wherein the at least two angeloyl groups or substituted two acids in side chains and cis to one another; or 30 wherein the at least two angeloyl groups or substituted two acids in side chains of alternate position carbon with trans or cis configuration at the structure.

14. The use of claim 1, wherein the compound has a triterpene or sapongenin backbone, wherein the backbone comprises a sugar moiety, wherein the sugar moiety 35 comprises glucose, or galactose, or rhamnose, or arabinose, or xylose, or alduronic 65 RECEIVED at IPONZ on 29 Mar 2011 acid, or glucuronic acid, or galacturonic acid or a combination thereof; wherein the backbone comprises two groups selected from the group consisting of acids with 5 carbons, angeloyl group, tigloyl group, senecioyi group, and combinations thereof attached to the backbone, wherein these two side groups are responsible for anticancer 5 or antiviral activity.

15. The compound of claim 3, wherein the compound has biangeloyl group, wherein the biangeloyl group is attached to a structure, and wherein the presence of the biangeloyl group is responsible for anticancer or antiviral activity. 10

16. A method for isolating compounds from Xanthoceras sorbifolia herb, or plants from the sapindaceae family, comprising the steps of: (a) extracting Xanthoceras sorbifolia or plant powder with organic solvents to obtain an organic extract; wherein the powder is prepared from the husks, branches, stems, 15 leaves, kernels, roots, barks or seed shells of the herb or plant. (b) collecting the organic extract; (c) refluxing the organic extract to obtain a second extract; (d) removing the organic solvent from the second extract; (e) drying and sterilizing the second extract to obtain a crude extract powder; 20 (f) fractionating the crude extract powder into components using HPLC and FPLC chromatography with silica gel, C18 or other equivalent solid phase materials; (g) selecting compounds that absorb the light at wavelength of 207nm or 254nm; (h) identifying and selecting the bioactive components of the crude extract powder with cytotoxic MTT method; 25 (i) purifying the bioactive components of the crude extract powder with FPLC to obtain a fraction of the bioactive components; and (j) isolating the compound from the fraction of the bioactive component with preparative HPLC. 30

17. A composition for inhibiting cancers cells growth, wherein the cancers include breast cancer, leukocyte cancer, liver cancer, ovarian cancer, bladder cancer, prostate cancer, skin cancer, bone cancer and brain cancer, comprising a compound of any of claims 3 to 4, and a pharmaceutically acceptable carrier. 35 66 </div>