NZ537291A - VEGFR-2 and VEGFR-3 inhibitory anthranylamidopyridines for the treatment of diseases caused by persistent angiogenesis - Google Patents

Abstract

VEGFR-2 and VEGFR-3 inhibitory anthranylamidopyridinamides of formula (I), the production and use thereof as medicaments for the treatment of diseases caused by persistent angiogenesis and intermediates for production of the compounds are disclosed, wherein the variables shown in formula (I) are as defined in the specification. These compounds can be used, for example, in tumour or metastasis growth, psoriasis, Kaposi's sarcoma, restenosis, such as for example, stent-induced restenoses, endometriosis, Crohn's disease, Hodgkin's disease, leukaemia, arthritis, such as rheumatoid arthritis, haemangioma, angiofibroma, eye disease, such as diabetic retinopathy, neovascular glaucoma, renal diseases, such as glomerulonephritis, diabetic nephropathy, malignant nephrosclerosis, thrombic microangiopathic syndrome, transplant rejection and glomerulopathy, fibrotic diseases, such as liver cirrhosis, mesangial cell proliferative diseases, artherosclerosis, injuries to nervous tissue and inhibition of the reocclusion of vessels after balloon catheter treatment, in vessel prosthetics, or after the application of mechanical devices to hold open vessels, such as for example, stents, as immune suppressants, as a support for scar-free wound healing, age spots and contact dermatitis. Said compounds may also be used as VEGFR-3 inhibitors in lymphangiogenesis.

Description

New Zealand Paient Spedficaiion for Paient Number 537291 537291 WO 2004/013102 PCT/EP2003/007964 VEGFR-2 and VEGFR-3 Inhibitory Anthranylamidopyridines The invention relates to VEGFR-2 and VEGFR-3 inhibitory anthranilamide pyridines, their production and use as pharmaceutical agents for treating diseases that are triggered by persistent angiogenesis as well as intermediate products for the production of compounds.

Persistent angiogenesis can be the cause or precondition of various diseases, such as tumor or metastasis growth, psoriasis; arthritis, such as rheumatoid arthritis, hemangioma, angiofibroma; eye diseases, such as diabetic retinopathy, neovascular glaucoma; renal diseases, such as glomerulonephritis, diabetic nephropathy, malignant nephrosclerosis, thrombic microangiopathic syndrome, transplant rejections and glomerulopathy; fibrotic diseases, such as cirrhosis of the liver, mesangial cell proliferative diseases and arteriosclerosis, or can result in an aggravation of these diseases.

Persistent angiogenesis is induced by the factor VEGF via its receptor. So that VEGF can exert this action, it is necessary that VEGF bind to the receptor, and a tyrosine phosphorylation is induced.

Direct or indirect inhibition of the VEGF receptor (VEGF = vascular endothelial growth factor) can be used for treating such diseases and other VEGF-induced pathological angiogenesis and vascular permeable conditions, such as tumor Intellectual Property Office of N.Z. 2<i NOV 2006 vascularization. For example, it is known that the growth of tumors can be inhibited by soluble receptors and antibodies against VEGF.

Anthranilamide pyridones that are used as pharmaceutical agents for treating psoriasis; arthritis, such as rheumatoid arthritis, hemangioma, angiofibroma; eye diseases, such as diabetic retinopathy, neovascular glaucoma; renal diseases, such as glomerulonephritis, diabetic nephropathy, malignant nephrosclerosis, thrombic microangiopathic syndrome, transplant rejections and glomerulopathy; fibrotic diseases, such as cirrhosis of the liver, mesangial cell proliferative diseases, arteriosclerosis, injuries to nerve tissue, and for inhibiting the reocclusion of vessels after balloon catheter treatment, in vascular prosthetics or after mechanical devices are used to keep vessels open, such as, e.g., stents, are known from WO 00/27820 (e.g., Example 38).

The compounds that are known from WO 00/27820 are generally effective in the indications cited, but their effectiveness is not very pronounced.

Anthranilic acid amides that are are highly effective but also have a good inhibition of the Cytochrome P 450 isoenzyme 3A4 are also known from WO 00/27819 (Example 2.54). The Cytochrome P 450 isoenzyme 3A4 is one of the essential metabolic enzymes via which pharmaceutical agents are degraded. An inhibition of this isoenzyme results in undesirable pharmaceutical agent interactions, especially in the case of multimorbid patients (patients with multiple disease conditions). There also exists the problem that in a combination therapy with other medications, increased toxicity occurs, which results from the inhibition of the degradation of the compounds and the associated excessive serum levels.

There is therefore the desire for active ingredients that on the one hand are effective and on the other hand are more compatible or do not exhibit any undesirable side effects.

There is therefore a desire for, on the one hand, more effective, and, on the other hand, more compatible compounds.

It has now been found that compounds of general formula I in which X stands for CH or N, W stands for hydrogen or fluorine, A, B, D, E and Q, in each case independently of one another, stand for a nitrogen or atom, whereby at least one of A, B, D, E and Q is a nitrogen atom, R stands for aryl or heteroaryl, which optionally can be substituted in one or more places in the same way or differently with halogen, hydroxy, C1-c12-alkyl, c3-c6-cycloalkyl, c3-c6-alkenyl, c2-c6-alkinyl, aralkyloxy, C1-c12- tntftltoctuai Property Office of N.Z.

IS NOV 2006 alkoxy, halo-Ci-C6-alkyl, cyano-Ci-C6-alkyl or with the group =0, -so2r6 or -OR5, whereby the Ci-C6-alkyl optionally also can be substituted with the group -OR5 or -NR9R10, Y and Z, in each case independently of one another, stand for a bond or for the group =C0, =CS or =S02, R and R , independently of one another, stand for hydrogen or for the group -CONR9R10, -S02R6, -COR11, -COCi-C6-alkyl, -CO-Ci-C6-alkyl-Rn, -NR9R10 or for Ci-C6-alkyl, C3-Cio-cycloalkyl, c3-c6-cycloalkenyl, aryl or heteroaryl that is optionally substituted in one or more places in the same way or differently with halogen, cyano, Ci-ci2-alkyl, Ci-Cn-alkoxy, hydroxy-Ci-C6-alkyl, halo-Ci-C6-alkyl or with the group -NR7R8, -OR5, -Ci-C6-alkyl-OR5, -SR4, -SOR4 or -S02R6, or R2, R3, Y and Z together with the nitrogen atom form a 3- to 8-membered saturated or unsaturated ring, which optionally can contain additional heteroatoms in the ring and optionally can be substituted in one or more places in the same way or differently with halogen, cyano, Ci-ci2-alkyl, Q-Cn-alkoxy, halo-Ci-c6-alkyl, hydroxy-C 1 -c6-alkyl, or with the group =0, -OR5, -SR4, -SOR4 or -so2r6, R4 stands for Ci-ci2-alkyl, aryl or heteroaryl, R5 stands for hydrogen, Ci-Ci2-alkyl, c3-Cio-cycloalkyl, Ci-ci2-alkoxy, halo- Ci-ci2-alkyl, or halo-c3-c6-cycloalkyl, R6 stands for hydrogen, Ci-ci2-alkyl, halo-Ci-ci2-alkyl, aryl or heteroaryl, or for the group -NR9R10, whereby the aryl or heteroaryl itself optionally can be substituted in one or more places in the same way or differently with Ci-ci2-alkyl, Ci-c6-alkoxy, halogen or halo-Ci-C6-alkoxy, R7 and R8, independently of one another, stand for hydrogen or Ci-ci2-alkyl, and R9 and R10, independently of one another, stand for hydrogen, Ci-C6-alkyl, c2-c6-alkenyl, aryl, c3-Cg-cycloalkyl or for the group -CONR7R8, or for Ci-ci2-alkyl that is optionally substituted in one or more places in the same way or differently with aryl, morpholino, hydroxy, halogen, C1-c12-alkoxy, or with the group -NR7R8, whereby the aryl itself optionally can be substituted in one or more places in the same way or differently with Ci-c6-alkoxy or halo-Ci-c6-alkyl, or R9 and R10 together form a 5- to 8-membered ring that can contain additional heteroatoms, and R11 stands for C1 -C6-alkyl, C1 -C6-alkoxy, hydroxy-C 1 -c6-alkyl, hydroxy-C 1 -c6-alkoxy, c3-c6-cycloalkyl, phenyl, pyridyl, biphenyl or naphthyl, whereby the phenyl itself can be substituted in one or more places in the same way or differently with Ci-C6-alkyl, or halo-Ci-C6-alkyl, as well as isomers, diastereomers, tautomers and salts thereof, exhibit improved properties, i.e., high effectiveness with simultaneously less CYP450 3A4 inhibition.

The compounds according to the invention prevent a tyrosine phosphorylation or stop persistent angiogenesis and thus the growth and propagation of tumors, whereby they are distinguished in particular by a slighter inhibition of isoforms of Cytochrome P 450 (3A4).

Medication with the compounds according to the invention can therefore also be done at no risk even without regard to pharmaceutical agents that are administered at the same time and that are degraded via these isoforms.

Alkyl is defined in each case as a straight-chain or branched alkyl radical, such as, for example, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, pentyl, isopentyl or hexyl, heptyl, octyl, nonyl, decyl, undecyl, or dodecyl.

Alkoxy is defined in each case as a straight-chain or branched alkoxy radical, such as, for example, methyloxy, ethyloxy, propyloxy, isopropyloxy, butyloxy, isobutyloxy, sec-butyloxy, pentyloxy, isopentyloxy, hexyloxy, heptyloxy, octyloxy, nonyloxy, decyloxy, undecyloxy or dodecyloxy.

Cycloalkyls are defined as monocyclic alkyl rings, such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, or cycloheptyl, cyclooctyl, cyclononyl or cyclodecyl, but also bicyclic rings or tricyclic rings, such as, for example, adamantanyl.

Cycloalkyl radicals can contain, instead of the carbon atoms, one or more heteroatoms, such as oxygen, sulfur and/or nitrogen. Those heterocycloalkyls with 3 to 8 ring atoms are preferred.

Cycloalkenyl is defined in each case as cyclobutenyl, cyclopentenyl, cyclohexenyl, cycloheptenyl, cyclooctenyl, cyclononenyl or cyclodecenyl, whereby the linkage can be carried out both to the double bond and to the single bonds.

Halogen is defined in each case as fluorine, chlorine, bromine or iodine.

Halo-alkyl, halo-alkoxy, etc., is defined in that the alkyl, alkoxy, etc., is substituted in one or more places, in the same way or differently, with halogen.

Alkenyl is defined in each case as a straight-chain or branched alkenyl radical that contains 2-6, preferably 4-6, C atoms. For example, the following radicals can be mentioned: vinyl, propen-l-yl, propen-2-yl, but-l-en-l-yl, but-l-en-2-yl, but-2-en-l-yl, but-2-en-2-yl, 2-methyl-prop-2-en-l-yl, 2-methyl-prop-l-en-l-yl, but-l-en-3-yl, but-3-en-l-yl, and allyl.

The aryl radical in each case comprises 3-12 carbon atoms and can in each case be benzocondensed.

For example, there can be mentioned: cyclopropenyl, cyclopentadienyl, phenyl, tropyl, cyclooctadienyl, indenyl, naphthyl, azulenyl, biphenyl, fluorenyl, anthracenyl, etc.

The heteroaryl radical in each case comprises 3-16 ring atoms, and instead of the carbon can contain one or more heteroatoms that are the same or different, such as oxygen, nitrogen or sulfur, in the ring, and can be monocyclic, bicyclic, or tricyclic, and in addition in each case can be benzocondensed.

For example, there can be mentioned: Thienyl, furanyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, triazolyl, thiadiazolyl, etc., and benzo derivatives thereof, such as, e.g., benzofuranyl, benzothienyl, benzoxazolyl, benzimidazolyl, indazolyl, indolyl, isoindolyl, etc.; or pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, etc., and benzo derivatives thereof, such as, e.g., quinolyl, isoquinolyl, etc.; or azocinyl, indolizinyl, purinyl, etc., and benzo derivatives thereof; or quinolinyl, isoquinolinyl, cinnolinyl, 8 phthalazinyl, quinazolinyl, quinoxalinyl, naphthyridinyl, pteridinyl, carbazolyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl, xanthenyl, or oxepinyl, etc.

The heteroaryl radical can be benzocondensed in each case. For example, there can be mentioned as 5-ring heteroaromatic compounds: thiophene, furan, oxazole, thiazole, imidazole, pyrazole and benzo derivatives thereof, and as 6-ring heteroaromatic compounds: pyridine, pyrimidine, triazine, quinoline, isoquinoline and benzo derivatives.

Heteroatoms are defined as oxygen, nitrogen or sulfur atoms.

A 3- to 8-membered ring in the meaning of R2, R3, Y and Z, which is formed together with the nitrogen atom, is defined as C3-Cg-cycloheteroalkyls and C3-C8-heteroaryls.

If an acid group is included, the physiologically compatible salts of organic and inorganic bases are suitable as salts, such as, for example, the readily soluble alkali salts and alkaline-earth salts as well as N-methyl-glucamine, dimethyl-glucamine, ethyl-glucamine, lysine, 1,6-hexadiamine, ethanolamine, glucosamine, sarcosine, serinol, tris-hydroxy-methyl-amino-methane, aminopropanediol, Sovak base, and l-amino-2,3,4-butanetriol.

If a basic group is included, the physiologically compatible salts of organic and inorganic acids are suitable, such as hydrochloric acid, sulfuric acid, phosphoric acid, citric acid, tartaric acid, fumaric acid, i.a.

The compounds of general formula I according to the invention also contain the possible tautomeric forms and comprise the E-isomers or Z-isomers, or, if a chiral center is present, also the racemates and enantiomers.

Those compounds of general formula I in which X stands for CH, W stands for hydrogen, A, B, D, E and Q as a ring together stand for pyridyl, R1 stands for aryl or heteroaryl, which optionally can be substituted in one or more places in the same way or differently with halogen, hydroxy, C1-C6-alkyl, c3-c6-cycloalkyl, c4-C6-alkenyl, C2-C6-alkinyl, aralkyloxy, C1-C6-alkoxy, halo-Ci-c6-alkyl, cyano-Ci-c6-alkyl, or with the group =0, -so2r6 or -OR5, whereby Ci-c6-alkyl optionally also can be substituted with the group -OR5 or -NR9R10, Y and Z, in each case independently of one another, stand for a bond, R2 and R3, independently of one another, stand for hydrogen or for the group -CONR9R10, -S02R6, -COR11, -COC,-C6-alkyl, -CO-Ci-C6-alkyl-Ru, -NR9R10 or for Ci-C6-alkyl, c3-c6-cycloalkyl, C3-C6-cycloalkenyl, aryl or heteroaryl that is optionally substituted in one or more places in the same way or differently with halogen, cyano, Ci-C6-alkyl, Ci-C6-alkoxy, hydroxy-C i-C6-alkyl, halo-Ci-C6-alkyl or with the group -NR7R8, -OR5, -Ci-C6-alkyl-OR5, -SR4, -SOR4 or -S02R6, or R2, R3, Y and Z together with the nitrogen atom form a 3- to 8-membered saturated or unsaturated ring, which optionally can contain additional heteroatoms in the ring and optionally can be substituted in one or more places in the same way or differently with halogen, cyano, C]-Ci2-alkyl, Ci-Ci2-alkoxy, halo-Ci-C6-alkyl, hydroxy-C i-C6-alkyl or with the group =0, -OR5, -SR4, -SOR4 or -S02R6, R4 stands for Ci-C6-alkyl, aryl or heteroaryl, R5 stands for hydrogen, C i -C6-alkyl, halo-C i -C6-alkyl, C i -C12-alkoxy, C3-Cio-cycloalkyl or halo-C3-C6-cycloalkyl, R6 stands for hydrogen, Ci-C6-alkyl, halo-C i-C6-alkyl, aryl or heteroaryl, or for the group -NR9R10, whereby the aryl or heteroaryl itself optionally can be substituted in one or more places in the same way or differently with Ci-C6-alkyl, Ci-C6-alkoxy, halogen or halo-C i-C6-alkoxy, R7 and R8, independently of one another, stand for hydrogen or Ci-C6-alkyl, R9 and R10, independently of one another, stand for hydrogen, Ci-C6-alkyl, C2-C6-alkenyl, aryl, C3-Cg-cycloalkyl, or for the group -CONR7R8, or for C1-C6-alkyl that is optionally substituted in one or more places in the same way or differently with aryl, morpholino, hydroxy, halogen or Ci-Ci2-alkoxy, or with the group -NR7R8, whereby the aryl itself optionally can be substituted in one or more places in the same way or differently with Ci-C6-alkoxy or halo-C i-C6-alkyl, and R11 stands for Ci-C6-alkyl, Ci-C6-alkoxy, hydroxy-Ci-C6-alkyl, hydroxy-Ci-C6-alkoxy, C3-C6-cycloalkyl, phenyl, pyridyl, biphenyl or naphthyl, whereby the phenyl itself can be substituted in one or more places in the same way or differently with Ci-C6-alkyl, or halo-C i-C6-alkyl, as well as isomers, diastereomers, tautomers and salts thereof, 11 have proven advantageous.

Those compounds of general formula I, in which X stands for CH, W stands for hydrogen, A, B, D, E, and Q as a ring together stand for pyridyl, R1 stands for phenyl, quinolinyl, isoquinolinyl or indazolyl, which optionally can be substituted in one or more places in the same way or differently with halogen, hydroxy, Ci-C6-alkyl, C2-C6-alkinyl, Ci-C6-alkoxy, halo-Ci-C6-alkyl, or cyano-Ci-C6-alkyl, whereby Ci-C6-alkyl optionally also can be substituted with the group -OR5 or -NR9R10, Y and Z, in each case independently of one another, stand for a bond, or for the group =CO, R2 and R3, independently of one another, stand for hydrogen or for the group -CONR9R10, -S02R6, -COR11, -COCi-C6-alkyl, -CO-Ci-C6-alkyl-Ru, -NR9R10 or for Ci-C6-alkyl or phenyl that is optionally substituted in one 7 8 or more places in the same way or differently with the group -NR R or -OR5, or R2, R3, Y and Z together with the nitrogen atom form a 3- to 8-membered saturated or unsaturated ring that optionally can contain additional heteroatoms in the ring and optionally can be substituted in one or more places in the same way or differently with halogen, cyano, Ci-Ci2-alkyl, Ci-Ci2-alkoxy, halo-Ci-C6-alkyl, hydroxy-C i-C6-alkyl or with the group =0, -OR5, -SR4, -SOR4 or -S02R6, R5 stands for hydrogen or Ci-C6-alkyl, R6 stands for hydrogen, Ci-C6-alkyl, halo-Ci-C6-alkyl, phenyl, benzyl, thiophenyl, or pyridyl, whereby the phenyl, benzyl, thiophenyl and pyridyl itself optionally can be substituted in one or more places in the same way or differently with C i -C6-alkyl, C i -C6-alkoxy, halogen or halo-C \ -C(,-alkoxy, 7 8 • R and R , independently of one another, stand for hydrogen or Ci-C6-alkyl, R9 and R10, independently of one another, stand for hydrogen, Ci-C6-alkyl, C2-C6-alkenyl, phenyl, biphenyl, C3-Cg-cycloalkyl, naphthyl or for the group 7 o -CONR R or for Ci-C6-alkyl that is optionally substituted in one or more places in the same way or differently with phenyl, morpholino, hydroxy, halogen, Ci-Ci2-alkoxy, or with the group -NR7R8, whereby the phenyl itself optionally can be substituted in one or more places in the same way or differently with Ci-C6-alkoxy or halo-C i-C6-alkyl, and R11 stands for C1 -C6-alkyl, C1 -C6-alkoxy, hydroxy-C 1 -C6-alkyl, hydroxy-C 1 -C6-alkoxy, C3-C6-cycloalkyl, phenyl, pyridyl, biphenyl or naphthyl, whereby the phenyl itself can be substituted in one or more places in the same way or differently with Ci-C6-alkyl, or halo-Ci -C6-alkyl, as well as isomers, diastereomers, tautomers and salts thereof, of special interest. 13 The compounds according to the invention as well as their physiologically compatible salts prevent a tyrosine phosphorylation or stop persistent angiogenesis and thus the growth and propagation of tumors, whereby they are distinguished in particular by a slighter inhibition of isoforms of Cytochrome P 450 (3A4). Medication using the compounds according to the invention can therefore be done at no risk even without regard to pharmaceutical agents that are administered at the same time and that are degraded via these isoforms.

The compounds of formula I as well as their physiologically compatible salts can be used as pharmaceutical agents based on their inhibitory activity relative to the phosphorylation of the VEGF receptor. Based on their profile of action, the compounds according to the invention are suitable for treating diseases that are caused or promoted by persistent angiogenesis.

Since the compounds of formula I are identified as inhibitors of the tyrosine kinases KDR and FLT, they are suitable in particular for treating those diseases that are caused or promoted by persistent angiogenesis that is triggered via the VEGF receptor or by an increase in vascular permeability.

The subject of this invention is also the use of the compounds according to the invention as inhibitors of the tyrosine kinases KDR and FLT.

Subjects of this invention are thus also pharmaceutical agents for treating tumors or use thereof.

The compounds according to the invention can be used either alone or in a formulation as pharmaceutical agents for treating tumor or metastasis growth, psoriasis, Kaposi's sarcoma, restenosis, such as, e.g., stent-induced restenosis, endometriosis, 14 Crohn's disease, Hodgkin's disease, leukemia; arthritis, such as rheumatoid arthritis, hemangioma, angiofibroma; eye diseases, such as diabetic retinopathy, neovascular glaucoma; renal diseases, such as glomerulonephritis, diabetic nephropathy, malignant nephrosclerosis, thrombic microangiopathic syndrome, transplant rejections and glomerulopathy; fibrotic diseases, such as cirrhosis of the liver, mesangial cell proliferative diseases, arteriosclerosis, injuries to nerve tissue, and for inhibiting the reocclusion of vessels after balloon catheter treatment, in vascular prosthetics or after mechanical devices are used to keep vessels open, such as, e.g., stents, as immunosuppressive agents, for supporting scar-free healing, in senile keratosis and in contact dermatitis.

In treating injuries to nerve tissue, quick scar formation on the injury sites can be prevented with the compounds according to the invention, i.e., scar formation is prevented from occurring before the axons reconnect. A reconstruction of the nerve compounds was thus facilitated.

The formation of ascites in patients can also be suppressed with the compounds according to the invention. VEGF-induced edemas can also be suppressed.

Lymphangiogenesis plays an important role in lymphogenic metastasizing (Karpanen, T. et al., Cancere Res. 2001 Mar 1,61(5): 1786-90, Veikkola, T., et al., EMBO J. 2001, Mar 15; 20 (6): 1223-31).

The compounds according to the invention now also show excellent action as VEGFR kinase 3 inhibitors and are therefore also suitable as effective inhibitors of lymphangiogenesis.

By a treatment with the compounds according to the invention, not only a reduction of the size of metastases but also a reduction of the number of metastases is achieved.

Such pharmaceutical agents, their formulations and uses are also subjects of this invention.

The invention thus also relates to the use of the compounds of general formula I for the production of a pharmaceutical agent or in the preparation of a composition for use as or for treatment of tumor or metastasis growth, psoriasis, Kaposi's sarcoma, restenosis, such as, e.g., stent-induced restenosis, endometriosis, Crohn's disease, Hodgkin's disease, leukemia; arthritis, such as rheumatoid arthritis, hemangioma, angiofibroma; eye diseases, such as diabetic retinopathy, neovascular glaucoma; renal diseases, such as glomerulonephritis, diabetic nephropathy, malignant nephrosclerosis, thrombic microangiopathic syndrome, transplant rejections and glomerulopathy; fibrotic diseases, such as cirrhosis of the liver, mesangial cell proliferative diseases, arteriosclerosis, injuries to nerve tissue, and for inhibiting the reocclusion of vessels after balloon catheter treatment, in vascular prosthetics or after mechanical devices are used to keep vessels open, such as, e.g., stents, as immunosuppressive agents, as a support in scar-free healing, in senile keratosis and in contact dermatitis.

The formation of ascites in patients can also be suppressed with the compounds according to the invention. VEGF-induced edemas can also be suppressed.

To use the compounds of formula I as pharmaceutical agents, the latter are brought into the form of a pharmaceutical preparation, which in addition to the active ingredient for enteral or parenteral administration contains suitable pharmaceutical, organic or inorganic inert carrier materials, such as, for example, water, gelatin, gum intellectual Property Office of N.Z. 21, NOV 2006 16 arabic, lactose, starch, magnesium stearate, talc, vegetable oils, polyalkylene glycols, etc. The pharmaceutical preparations can be present in solid form, for example as tablets, coated tablets, suppositories, or capsules, or in liquid form, for example as solutions, suspensions or emulsions. They optionally contain, moreover, adjuvants such as preservatives, stabilizers, wetting agents or emulsifiers, salts for changing osmotic pressure or buffers.

For parenteral administration, especially injection solutions or suspensions, especially aqueous solutions of the active compounds in polyhydroxyethoxylated castor oil, are suitable.

As carrier systems, surface-active adjuvants such as salts of bile acids or animal or plant phospholipids, but also mixtures thereof as well as liposomes or components thereof can also be used.

For oral administration, especially tablets, coated tablets or capsules with talc and/or hydrocarbon vehicles or binders, such as for example, lactose, corn starch or potato starch, are suitable. The administration can also be carried out in liquid form, such as, for example, as juice, to which optionally a sweetener or, if necessary, one or more flavoring substances, is added.

The dosage of the active ingredients can vary depending on the method of administration, age and weight of the patient, type and severity of the disease to be treated and similar factors. The daily dose is 0.5-1000 mg, preferably 50-200 mg, whereby the dose can be given as a single dose to be administered once or divided into 2 or more daily doses. 17 The above-described formulations and forms for dispensing are also subjects of this invention.

The production of the compounds according to the invention is carried out according to methods that are known in the art. For example, compounds of general formula I are obtained in that a compound of general formula II in which A, B, D, E, Q, W, X and R1 have the meanings that are indicated in general formula I, and M stands for halogen, first is converted into an amine and then is acylated, or M is substituted by an NHCOR' group.

W O B M (H), 18 Compounds of general formula I are also obtained in that a compound of general formula Ila, (Ha), in which Ry stands for Ci-C6-alkyl or hydrogen, and FG means a leaving group, such as, e.g., halogen, O-triflate, O-mesylate, O-tosylate or sulfone, first is converted into an amide, and then the leaving group is substituted by an N(Y-R2)-R3 group, or a compound III -R N (III), in which R2, R3, Y and Z have the meanings that are indicated in general formula I and Ry stands for Ci-C6-alkyl or hydrogen, first is saponified and then is converted into the amide. 19 The amide formation is carried out according to methods that are known in the literature.

For amide formation, it is possible to start from a corresponding ester. The ester is reacted according to J. Org. Chem. 1995, 8414 with aluminum trimethyl and the corresponding amine in solvents such as toluene at temperatures of 0°C to the boiling point of the solvent. If the molecule contains two ester groups, both are converted into the same amide. Instead of aluminum trimethyl, sodium hexamethyldisilazide can also be used.

For amide formation, however, all processes that are known from peptide chemistry are also available. For example, the corresponding acid can be reacted with the amine in aprotic polar solvents, such as, for example, dimethylformamide, via an activated acid derivative, that can be obtained, for example, with hydroxybenzotriazole and a carbodiimide, such as, for example, diisopropylcarbodiimide, at temperatures of between 0°C and the boiling point of the solvent, preferably at 80°C. The reaction between carboxylic acid and amine, however, can also be produced by activation reagents, such as HATU (N-dimethylamino-lH-l,2,3-triazolo-[4,5-b]pyridin-l-yhnethylene]-N-methyhnethanaminiuiri hexafluorophosphate-N-oxide), whereby polar aprotic solvents, such as, for example, dimethylformamide, are suitable for the reaction. The addition of a base such as N-methylmorpholine is necessary. The reaction proceeds at temperatures of 0-100°C, whereby the procedure is preferably performed at room temperature, but in many cases heating is indispensable. For the amide formation, the process can also be used with the acid halide, the mixed acid anhydride, imidazolide or azide. A previous protection of an additional amino group, for example as an amide, is not necessary in all cases, but can advantageously influence the reaction.

In the case of bisacid chlorides, cyclic compounds can be produced. Also, in the case of halogen acid halides, cyclic compounds can be produced. The ring closure is then completed optionally by adding a strong base, such as, for example, sodium alcoholates. The same thing holds true for the sulfonic acid halides, whereby double sulfonations can also occur.

Ureas are produced from amino compounds by reaction with isocyanates. Inert solvents such as methylene chloride or else dimethylformamide at temperatures from room temperature up to 100°C, preferably at 60°C are used. Pressure is advantageous for the reaction.

The reaction of halopyridines with amides is carried out under catalysis, for example by palladium or copper catalysis. In the case of copper catalysis (literature, see Synlett. 2002,427), solvents such as dioxane or dimethylformamide are used at temperatures up to the boiling point of the solvent, preferably 120°C. As a base, potassium phosphate or else cesium carbonate is used. Ethylenediamine is advantageous for complexing the copper(I) iodide that is used as a catalyst An application of pressure is not harmful. In the case of palladium catalysis, both paUadium(II) salts, such as palladium(II) acetate, and palladium(O) complexes, such as palladium(0)2dibenzylidene acetone3 (literature, see JACS 2002,6043, THL 1999,2035, Org. Lett 2001,2539, THL 2001,4381 or THL 2001, 3681) can be used. As a solvent, toluene, dioxane or dimethylformamide is used at temperatures from room temperature up to the boiling point of the solvent, preferably around 100°C. As a co-ligand, BINAP, DPPF or Intellectual Property Office of N.Z. 24 NOV 2006 21 Xanthphos is used. A base is also necessary. To this end, cesium carbonate, potassium phosphate or else sodium-t-butylate is used. These components can be combined in various ways.

The production of the pyridinamines from the corresponding 2-halopyridines is carried out in solvents such as pyridine or in protic polar solvents such as ethylene glycol at temperatures up to 200°C. Catalysis by copper(I) salts can be necessary for the reaction. The application of pressure is absolutely necessary in the case of the reaction of low-boiling amines, but can also be used advantageously in the conventional amines.

The ether cleavage is accomplished according to known methods, for example by reaction with boron tribromide in inert solvents, such as methylene chloride, at temperatures of -78°C up to room temperature, preferably at -78°C.

The compounds of general formulas II, Ila and III, W O M (II), 22 N (lla) und (HI), z Y R2 [and] in which A, B, D, E, Q, W, X, Y, Z, R2, and R3 have the meanings that are indicated in general formula I and M stands for halogen, FG stands for a leaving group, such as, e.g., halogen, O-triflate, O-mesylate, O-tosylate or sulfone, and RY stands for Ci-C6-alkyl or hydrogen, represent valuable intermediate products for the production of the compounds of general formula I according to the invention and are thus also subjects of this invention. 23 Production of the Compounds According to the Invention The following examples explain the production of the compounds according to the invention without the scope of the claimed compounds being limited to these examples.

Example 1.0 Production of 2-{[2-(2-Dimethylamino-ethylamino)-pyridin-4-ylmethyI]-amino}-Ar-(3-trifluoromethyl-phenyl)-benzamide 90 mg (0.2 mmol) of 2-[(2-bromo-pyridin-4-y lmethy l)-amino] -N-(3 -trifluoromethyl-phenyl)-benzamide is dissolved in 3 ml of pyridine and mixed with 1 ml of N,N-dimethyl-aminoethylamine and heated in a pressure vessel for 5 hours to a bath temperature of 200°C. After cooling, it is concentrated by evaporation, and 90 mg of 2-{[2-(2-dimethylamino-ethylamino)-pyridin-4-ylmethyl]-amino}-iV-(3-trifluoromethyl-phenyl)-benzamide is obtained.

Melting point: 100°C 24 Similarly produced are also the following compounds: Typ a [Key: Typ = Type] Example No.

Type R2 R1 MW Melting Point [°C] or MS Molar Peak (m/e) 1.1 A -(CH2)2-OH H .XK 430.5 1.2 A -(CH2)2-OH H JCO * 413.5 130-132 1.3 A -(CH2)3OH H .XX, 444.5 148 1.4 A -(CH2)4OH H .XI, 458.5 124 1.5 A -(CH2)5OH H .XX, 472.5 70 1.6 A -(CH2)2OMe H .XX, 444.5 Example No.

Type R2 R3 R1 MW Melting Point [°C] or MS Molar Peak (m/e) 1.7 A oh ■vA H .jo. 444.5 80 1.8 A oh •VsA H .iX, 444.5 65 1.9 A H 444.5 81 26 Example No.

Type R2 H3 R1 MW Melting Point [°C1 or MS Molar Peak (m/e) 1.10 A (CH2)3N Me2 H .XX, 471.5 68 1.11 B -(CH2)2-OH H xo * 413.5 Resin 1.12 A Phenyl H .XX. 462.5 1.13 A -(CH 2)5- xo 437.54 1.14 A -(CH2)2-0-(CH2)2- * 439.52 174 1.15 A -(CH2)2-NMe-(CH2)2- JGO * 452.56 85 1.16 A -(CH2)2-S-(CH2)2- JCO * 455.58 158 1.17 A -(CH2)2-S02-(CH2)2- xx> * 487.58 1.18 A -(CH2>4- JCO * 423.52 148 27 Example 2.0 Production of 2-|(2-Amino-pyridin-4-ylmethyl)-amino]-7V-(3-trifluoromethyl-phenyl)-benzamide 8.747 g (19.4 mmol) of 2-[(2-bromo-pyridin-4-ylmethyl)-amino]-A'-(3-trifluoromethyl-phenyl)-benzamide is heated with 175 mg of copper(I) oxide in 150 ml of ethanediol for 23 hours under 10 bar of ammonia pressure to 80°C in an autoclave. After the solvent is distilled off in a vacuum, the residue is purified on silica gel with a gradient of ethyl acetate.ethanol = 100:0 to 0:100 as an eluant. 4.15 g (51% of theory) of 2-[(2-amino-pyridin-4-ylmethyl)-amino]-Ar-(3-trifluoromethyl-phenyl)-benzamide with a melting point of 64°C is obtained.

Similarly produced are: 28 Example 2.1 2-1(2-Amino-pyridin-4-ylmethyl)-amino]-/V-isoquinolin-3-yl-benzainide Melting point: 202°C Example 2.2 2-[(2-Amino-pyridin-4-ylmethyl)-amino]-Ar-(l/f-indazol-5-yl)-benzamide MS: m/e 358 Melting point: 200°C 29 Example 2.3 2-[(2-Amino-pyridin-4-ylmethyl)-amino]-./V-(2-methyl-2//-indazol-6-yI)-benzamide MW: 372.43 Example 3.0 Production of 2- {[2-(3-Benzyl-ureido)-pyridin-4-ylmethy 1] -amino} -iV-(3-trifluoromethyl-phenyl)-benzamide 100 mg (0.26 mmol) of 2-[(2-amino-pyridin-4-ylmethyl)-amino]-Ar-(3-trifluoromethyl-phenyl)-benzamide is mixed in 2.5 ml of methylene chloride with 37.9 mg (0.29 mmol) of benzyl isocyanate, and it is stirred overnight at room temperature.

After concentration by evaporation, the residue is chromatographed. 66 mg (49% of theory) of 2- {[2-(3 -benzyl-ureido)-pyridin-4-ylmethyl]-amino} -N-(3 -trifluoromethyl-phenyl)-benzamide with a melting point of 153°C is obtained.

Similarly produced are also the following compounds: [Key: Typ = Type] Example No.

Type R1 R1U MW Melting Point [°C] or MS Molar Peak (m/e) 3.1 A .JX Phenyl 505.5 185 3.2 A .JX Ph(CH2)2- 533.5 76 3.3 A .JX n-Butyl 485.5 84 31 Example No.

Type R1 RlU MW Melting Point [°C] or MS Molar Peak (m/e) 3.4 A .XX, OMe ,xic 595.5 206 3.5 A .XX, .Xx0F, 573.5 186 3.6 A .xx. jcr * 573.5 211 3.7 A .XX.

Ethyl 457.5 154 3.8 A XX, * 581.6 195 3.9 A XX, 555.5 180 3.10 A .XX, -ch3 443.4 159 3.11 A .JX -ch2ch2ci 491.9 157 3.12 A .XX. n-Propyl 471.5 80 32 Example No.

Type R1 R1U MW Melting Point [°C] or MS Molar Peak (m/e) 3.13 a .JX, i-Propyl 471.5 96 3.14 a .JX. vO • 497.5 103 3.15 a JX, -conh2 472.4 190 3.16 a .JCO OMe .A: 578.6 213 3.17 a JCO * .JX. 556.5 203 3.18 a JCO * ja" * 556.5 165 3.19 a JCO * JO * 488.5 198 3.20 a J30 * 538.6 213 3.21 a JCO * •^o 502.6 185 33 Example No.

Type R1 Rl« MW Melting Point [°C] or MS Molar Peak (m/e) 3.22 a JCO * ^X) * 516.6 171 3.23 a JCO * -ch2ch2c1 474.9 195 3.24 a JCO • -ch3 426.5 225 3.25 a JCO * n-Propyl 454.5 3.26 a JCO * i-Propyl 454.5 3.27 a JCO * Ethyl 440.5 3.28 a JCO « x> * 480.6 205 3.29 a JCO * -CONH2 455.5 129 Examples 3.15 and 3.29 are produced analogously to Example 3.0 with use of trimethylsilyl isocyanate. 34 Example 3.30 Production of 2- {[2-(3,3-Dimethyl-ureido)-pyridin-4-ylmethyl] -amino} -iV-(3-isoquinolinyl)-benzamide 100 mg (0.23 mmol) of 2-[(2-bromopyridin-4-ylmethyl)-amino]-JV-(3-isoquinolinyl)-benzamide is heated in 2 ml of dioxane with 89 mg (0.28 mmol) of cesium carbonate, 61 mg (0.69 mmol) of N,N-dimethylurea, 4.7 mg (0.0046 mmol) of dipalladium-tribenzylidene acetone and 7.9 mg (0.014 mmol) of Xanthphos under a cover gas and in a moisture-free environment for 9 hours to a bath temperature of 100°C. It is then mixed with 20 ml of methylene chloride, suctioned off and concentrated by evaporation. The residue is chromatographed on silica gel with ethyl acetate as an eluant. 24 mg (24% of theory) of 2-{[2-(3,3-dimethyl-ureido)-pyridin-4-ylmethyl]-amino}-N-(3-isoquinolinyl)-benzamide is obtained.

(MS (CI): 441 (M++H)) Similarly produced are also the following compounds: R H I N 0 Typ A [Key: Typ = Type] Example No.

Type R1 R9 R10 MW Melting Point [°C] or MS Molar Peak (m/e) 3.31 A JCO * H H 412.5 222 3.32 A .JX.

H H 429.4 3.33 A XA * Me H 415.46 3.34 A .JX Me H 415.46 110-113 3.35 A .JX Me Me H 429.48 230-232 36 Example No.

Type R1 R" rM MW Melting Point [°C] or MS Molar Peak (m/e) 3.36 A XX/-"- * Me H 429.48 130-133 3.37 A .i-Prop JUL/ * Me H 457.54 3.38 A .

H 455.52 3.39 A XX> Me .

H 455.52 3.40 A .XQ Me Me Me 443.51 3.41 A * Me Me 443.51 3.42 A xx> * \ Et Me H 443.51 3.43 A XX( * \. „ i-Prop Me H 457.54 3.44 A jQ^N-Et * Me H 443.51 3.45 A XX"-""05 * Me H 457.54 37 Example No.

Type R1 R> RlU MW Melting Point [°C] or MS Molar Peak (m/e) 3.46 A jCO-"- * Me H 457.54 3.47 A rY-r* AJJ1 * Me H 473.53 199.5 3.48 A JJL> • Me H 443.51 208.8 3.49 A .JC Me H 453.50 242 3.50 A * Me H 429.48 m/e 429 3.51 A Me ,o> * Me H 429.48 205.1 3.52 A Me cycl.Prop H 455.52 192 3.53 A * cycl.Prop H 455.52 216 3.54 A no- VA OMe Me H 473.53 247 38 Exam Type R1 R^ R1U MW Melting Point ple No.

[°C] or MS Molar Peak (m/e) 3.55 A XXy* * •^o H 499.57 3.56 A .XX'-u Me H 473.53 3.57 A .XX>^» Me H 473.53 3.58 B XC"-"* * Me H 429.48 3.59 B jCQ1 * \ Me Me H 429.48 3.60 A XC^c, * Me H 454.48 3.61 A y—CU xx> * Me H 454.48 3.62 A * Me Me H 486.53 3.63 A * -(CH2)2-0-CH3 H 473.53 3.64 A XC"-"* * -(CH2)2N(CH3)2 H 486.58 39 Example No.

Type R1 R' RlU MW Melting Point [°C] or MS Molar Peak (m/e) 3.65 A XX>-"' * "^O H 528.61 3.66 A xx> \» -(CH2)2-0-CH3 H 473.53 3.67 A XE> L -(CH2)2N(CH3)2 H 486.58 3.68 A ,XX> Me '^O H 528.61 3.69 A xx> Me H 454.49 40 Example 4.0 Production of 2-[(2-Methanesulfonylamino-pyridin-4-ylmethyl)-amino]-iV-(3-trifluoromethyl-phenyl)-benzamide 90 mg (0.2 mmol) of 2- [(2-bromo-pyridin-4-y lmethyl)-amino] -N-(3 -trifluoromethyl-phenyl)-benzamide and 23 mg (0.24 mmol) of methanesulfonic acid amide are introduced into 5 ml of dioxane and mixed in succession with 4 mg (0.02 mmol) of copper(I) iodide, 85 mg (0.4 mmol) of potassium phosphate and 2 mg (0.02 mmol) of ethylenediamine. After 1 hour of stirring at a bath temperature of 120°C, it is diluted with 20 ml of water and concentrated by evaporation. It is then made alkaline with ammonia and shaken out three times with 25 ml each of ethyl acetate. The collected organic phase is washed with water, dried, filtered and concentrated by evaporation. The residue is made crystalline with ethyl acetate and a little hexane, stirred and suctioned off. 24 mg (26% of theory) of 2-[(2-methanesulfonyl-amino-pyridin-4-ylmethyl)-amino]-iV-(3-trifluoromethyl-phenyl)-benzamide with a melting point of 214.5°C is obtained. 41 Similarly produced are: Example No.

R1 R2 MW Melting Point [°C] or MS Molar Peak (m/e) 4.1 .XX, sO 526.54 259.2 4.2 .JX 541.55 >300 4.3 .JX. 610.53 248.6 4.4 .XX, -SO2CF3 518.44 238.9 4.5 JCO * -SO2CH3 447.52 m/e: 447 4.6 .JX 562.52 252.5 4.7 .JX. ,^-s°2h( —OnBu 598.64 231 42 Example No.

R1 R2 MW Melting Point [°C] or MS Molar Peak (m/e) 4.8 .XX.

/S02^S * \J~" 567.01 255.2 4.9 JGQ * /S°VS\ * Oci 550.06 234.7 4.10 .-XX, 540.56 245.7 4.11 ./s°^0 540.56 194.8 4.12 J& * 498.57 256 4.13 .jOi 512.59 219 43 Example 5.0 Production of 2-[(2-Bismethanesulfonylamino-pyridin-4-ylmethyl)-amino]-jV-(3 trifluoromethyl-phenyl)-benzamide 193 mg (0.5 mmol) of 2-[(2-amino-pyridin-4-ylmethyl)-amino]-N-(3-trifluoromethyl-phenyl)-benzamide is mixed in 3 ml of dichloromethane with 69 mg (0.6 mmol) of methanesulfonic acid chloride and 61 mg (0.6 mmol) of triethylamine and stirred together for 1.5 hours at room temperature. Then, it is washed once with dilute sodium bicarbonate solution, dried, filtered and concentrated by evaporation. The residue is chromatographed via flash chromatography (5 g of Isolute) with a gradient of cyclohexane:ethyl acetate =100:0 to 50:50 as an eluant. 80 mg (30% of theory) of 2-[(2-bismethanesulfonylamino-pyridin-4-ylmethyl)-amino]-Ar-(3-trifluoromethyl-phenyl)-benzamide is obtained as a resin.

(MS: m/e 542) 44 Example 6.0 Production of 2-[(2-Butyrylamino-pyridin-4-ylmethyl)-amino]-iV-(3-isoquinolinyl) benzamide 100 mg (0.23 mmol) of 2-[(2-bromopyridin-4-ylmethyl)-amino]-iV-(3-isoquinolinyl)-benzamide is heated in 1 ml of dioxane with 89 mg (0.28 mmol) of cesium carbonate, 24 mg (0.69 mmol) of butyramide, 4.7 mg (0.0046 mmol) of dipalladium-tribenzylidene acetone and 7.9 mg (0.014 mmol) of Xanthphos under a cover gas and in a moisture-free environment for 25 hours to a bath temperature of 90°C. It is then mixed with 20 ml of methylene chloride, suctioned off and concentrated by evaporation. The residue is chromatographed on silica gel first with hexane, then with hexane :ethyl acetate = 8:2 and then with hexane:ethyl acetate = 1:1 as an eluant. 45 mg (42% of theory) of 2-[(2-butyrylamino-pyridin-4-ylmethyl)-amino]-Ar-(3 -isoquinolinyl)-benzamide with a melting point of 173°C is obtained. 45 Similarly produced are: R2 N Example No.

R1 R2 MW Melting Point [°C] or MS Molar Peak (m/e) 6.1 JX.

-COn-Prop 456.47 168 6.2 jcq * -COMe 411.46 220 6.3 JCO * -COEt 425.49 183 6.4 JCO * -COn-Bu 453.54 167 6.5 V o o \ 437.50 218 6.6 JOO * 549.63 212 46 Example No.

Rl R2 MW Melting Point [°C] or MS Molar Peak (m/e) 6.7 XQ * ^CO-^L- 453.54 112 6.8 JCQ * ./cohOH 529.64 219 6.9 JDD * 4/ ./co^O 523.59 215 6.10 * -COt-Bu 453.54 91 6.11 .JX. ./co^O~ 546.59 111 6.12 * -COOEt 441.50 185 6.13 .JO,, COMe 428.41 185 6.14 .Xt> CO-cycl.Prop 426.48 210-212 6.15 * CO-cycl.Prop 426.48 127-128 6.16 .XI,, 558.48 47 Example No.

R1 R2 MW Melting Point I°C] or MS Molar Peak (m/e) 6.17 .JX COPh 490.48 6.18 .JX,. ./co-C$ 508.47 6.19 XX>-' CO-cycl.Prop 440.51 114-115 6.20 JCO * CO-(CH2)4-OH 469.54 136 6.21 xx> * \ Me -COOEt 444.49 205-210 6.22 .JX- -COOEt 430.47 6.23 jyy * \ Me C02CH2(i-Prop) 472.55 187 6.24 xx> * \ Me C02(i-Prop) 458.52 204 6.25 * \ Me CO-cycl.Prop 440.51 105-107 6.26 .JX /= N 491.47 48 Example No.

R1 R2 MW Melting Point [°C] or MS Molar Peak (m/e) 6.27 jC& * COOEt 430.47 213 6.28 XO"" * COOEt 444.49 194-196 6.29 .JX.

CO-cycl-Prop 545.45 213 6.30 CO-t-Bu 470.49 155 6.31 xo * -CO-CH2-O-(CH2)2OH 471.51 86 49 Example 6.32 Similarly produced is: 2-{[2-(Acetyl-methyl-amino)-pyridin-4-ylmethyl]-amino}-Ar-isoquinolin-3-yl-benzamide Melting point 71 °C Example 7.0 Production of 2-{[2-(2-Oxo-pyrrolidin-l-yl)-pyridin-4-ylmcthyl]-amino}-7V-(3-trifiuoromethyl-phenyl)-benzamide 156 mg (0.5 mmol) of 2-{[2-(2-oxo-pyrrolidin-l-yl)-pyridin-4-ylmethyl]-amino}-benzoic acid is mixed in 5 ml of dimethylformamide with 0.12 ml (1 mmol) of 3-aminobenzotrifluoride, 228 mg (0.6 mmol) of HATU ( N-dimethylamino-1H-1,2,3-triazolo-[4,5-b]pyridin-l-ylmethylene]-N-methylmethanammoniumhexafluorophosphate-N-oxide) and 0.14 ml of N-methylmorpholine, and it is stirred overnight at room 50 temperature. It is diluted with ethyl acetate and washed in succession with saturated sodium bicarbonate solution, water and saturated common salt solution. The organic phase is dried, filtered and concentrated by evaporation. The residue is chromatographed on Isolute as a mobile solvent. 95 mg (42% of theory) of 2-{[2-(2-oxo-pyrrolidin-l-yl)-pyridin-4-ylmethyl]-amino}-iV-(3-trifluoromethyl-phenyl)-benzamideis obtained.

(MS: m/e 454) Similarly produced are: R2, R3, Y and Z = G Example No.

R1 G MW Melting Point [°C] or MS Molar Peak (m/e) 7.1 JCO « 439.5 189°C 51 Example No.

R1 G MW Melting Point [°C] or MS Molar Peak (m/e) 7.2 .XX. <3 1 « 455.4 m/e 455 7.3 .XX x . —N^N'Me 469.5 209 7.4 JCO * I . —N NH VJ 438.5 154 7.5 .XX) -A 435.5 217.3 7.6 ,crf- * 1 « 426.48 195-200 7.7 -6 426.48 105-110 52 Example 8.0 Produced similarly to Example 6.0 is: 2-{[2-(2-Hydroxymethyl-5-oxo-pyrrolidin-l-yl)-pyridin-4-ylmethyl]-amino}-iV-isoquinolin-3-yl-benzamide Similarly produced to this are: 53 Example No.

R1 G MW Melting Point [°C] or MS Molar Peak (m/e) 8.1 JCG * ^OH 467.53 98°C 8.2 * '-P 467.53 76°C 8.3 JCO * X OH 467.53 95 °C 8.4 JCO * -6 447.50 86°C 8.5 JCO * 481.94 186°C 8.6 XX? * \ Me -•& 440.51 196-198 8.7 XX'"-ue * 440.51 100 (Dec.) 8.8 XXX"" « 440.51 159 8.9 Me JO* * -A 440.51 54 Example No.

R1 G MW Melting Point [°C] or MS Molar Peak (m/e) 8.10 XX/-m * o x . — N NH 441.49 8.11 JCC"-"* * I « 441.49 8.12 Me „a> * I . —N^ NH 441.49 8.13 jCO- * \ Me O x . — N NH 441.49 8.14 * 454.33 8.15 JCQ * \ Me ~6 454.33 8.16 xo-- * -6 454.33 8.17 Me Xx> * -6 454.33 8.18 jCO,-w * 468.56 8.19 ^i-Prop JO> * -■6 468.56 55 Example 9.0 Production of 2-{[2-(2,5-Dioxo-pyrrolidin-l-yl)-pyridin-4-yImethyl]-amino}-iV-(3-trifluoromethyl-phenyl)-benzamide 193 mg (0.5 mmol) of 2-[(2-ammo-pyridm-4-ylmethyl)-amino]-iV-(3-trifluoromethyl-phenyl)-benzamide is mixed in 20 ml of dichloromethane with 0.21 ml (1.5 mmol) of triethylamine, and it is mixed at room temperature drop by drop with a solution of 93 mg (0.6 mmol) of succinic acid dichloride in 3 ml of methylene chloride. After stirring overnight at room temperature, it is diluted with methylene chloride and washed in succession with water, saturated sodium bicarbonate solution and saturated common salt solution. Then, the organic phase is dried, filtered and concentrated by evaporation. The residue is chromatographed on Isolute (Separtis Company) with a gradient of methylene chloride:ethanol = 100:0 to 95:5. 120 mg (51% of theory) of 2-{[2-(2,5-dioxo-pyrrolidin-1 -yl)-pyridin-4-ylmethyl]-amino} -N-( 3 -trifluoromethyl-phenyl)-benzamide is obtained.

(MS: m/e 468) 56 Similarly produced is: Example 9.1 2-{[2-(3,5-Dioxo-morpholin-4-yl)-pyridin-4-ylmethyl]-amino}-iV-(3-trifIuoromethyl-phenyl)-benzamide N O Melting point 201.9°C 57 Example 10.0 Production of 2-[(2-(3-Chloropropanesulfonylamino-pyridin-4-ylmethyl)-amino]-iV-(3-trifluoromethyl-phenyl)-benzamide 135 mg (0.35 mmol) of 2-[(2-amino-pyridin-4-ylmethyl)-amino]-iV-(3-trifluoromethyl-phenyl)-benzamide is mixed in 10 ml of dichloromethane with 62 mg (0.35 mmol) of 3-chloropropanesulfonic acid chloride and 49 fil (0.35 mmol) of triethylamine, and it is stirred for 2 hours at room temperature. Then, it is washed once with saturated sodium bicarbonate solution, filtered and concentrated by evaporation. The residue is chromatographed via flash chromatography (5 g of Isolute) with a gradient of dichloromethane:ethanol = 100:0 to 90:10 as an eluant. 67 mg (36% of theory) of 2-[(2-(3-chloropropanesulfonylamino-pyridin-4-ylmethyl)-amino]-N-(3-trifluoromethyl-phenyl)-benzamide is obtained.

(MS (CI): 491 (100%, M++H-HC1)) 58 Example 11.0 Production of 2-{[2-(l,l-Dioxo-lA,6-isothiazolidin-2-yl)-pyridin-4-ylmethyl]-amino}-iV-(3-trifluoromethyl-phenyl)-benzamide 58 mg (0.11 mmol) of 2-[(2-(3-chloropropanesulfonylamino-pyridin-4-ylmethyl)-amino]-N-(3-trifluoromethyl-phenyl)-benzamide is suspended in 5 ml of ethanol and mixed with 5 mg of sodium hydride (55% in mineral oil). The mixture is refluxed for 1 hour, mixed with 10 ml of water and extracted with ethyl acetate. The aqueous phase is saturated with sodium sulfate and extracted repeatedly by stirring with ethyl acetate overnight. After the combined extracts are concentrated by evaporation, 50 mg (93% of theory) of 2-{[2-( 1,1 -dioxo-1 X6-isothiazolidin-2-yl)-pyridin-4-ylmethyl]-amino}-Ar-(3-trifhioromethyl-phenyl)-benzamide is obtained.

(MS (CI): 491 (100%, M++H)) 59 Example 12.0 Ar-[2-(2-Hydroxy-ethyl)-2//-indazoI-5-yl]-2-{[2-(3-methyl-ureido)-pyridin-4-ylmethyl]-amino}-benzamide 50 mg (0.11 mmol) of Ar-[2-(2-methoxy-ethyl)-2//-indazol-5-yl]-2-{[2-(3-methyl-ureido)-pyridin-4-ylmethyl]-amino}-benzamide is introduced into 5 ml of methylene chloride and mixed drop by drop under argon and in a moisture-free environment at -78°C with 0.56 ml of boron tribromide (1 molar in methylene chloride). It is stirred for 15 more minutes, the cold bath is removed, and it then is stirred for 2 more hours. Then, it is mixed with water, the methylene chloride is drawn off, made alkaline with sodium bicarbonate solution and extracted twice with 15 ml each of ethyl acetate. The collected organic phase is dried, filtered, and concentrated by evaporation. The residue is chromatographed on silica gel with a gradient of methylene chloriderethanol = 100:0 to 90:10 as an eluant, and 27 mg of iV-[2-(2-hydroxy-ethyl)-2#-indazol-5-yl]-2-{[2-(3-methyl-ureido)-pyridin-4-ylmethyl]-amino }-benzamide is obtained. 60 Similarly produced from the corresponding methoxy compounds are: Example No.

R1 R2 MW Melting Point [°C] or MS Molar Peak (m/e) 12.1 XX/-"* * 459.51 187 12.2 OH ■-V 459.51 228 12.3 * 459.51 229 12.4 /\.°h r^5v-'N * •Ar 459.51 12.5 xx> * \ Me 459.51 220 61 Production of Intermediate Compounds Example A If the production of intermediate compounds is not described, the latter are known or can be produced analogously to known compounds or to processes that are described here.

Stage 1 a) Production of 2-Bromopyridine-5-carbaldehyde o is carried out according to F. J. Romero-Salguerra et al. THL 40,859 (1999). b) Production of 2-Bromo-isonicotinic Acid 160 g (0.93 mol) of 2-bromo-4-methyl-pyridine is added in drops to 152 g (0.96 mol) of potassium permanganate in 41 of water. Then, it is stirred under reflux for one 62 hour before 152 g (0.96 mol) of potassium permanganate is added once more. After two additional hours of stirring under reflux, it is suctioned off in a hot state over Celite and washed with water. The aqueous phase is shaken out three times with dichloromethane. The aqueous phase is concentrated by evaporation to one-half its original volume, and the pH is set at 2 with concentrated hydrochloric acid. The precipitated solid is suctioned off and dried at 70°C in a vacuum. 56.5 g of white solid product accumulates.

Production of 2-Bromo-4-hydroxymethyl-pyridine .2 ml (295 mmol) of triethylamine is added to 56.5 g (280 mmol) of 2-bromo-isonicotinic acid in 1.21 of THF. Then, it is cooled to -10°C and mixed drop by drop with 38.2 ml (295 mmol) of isobutyl chloroformate. After stirring has been continued for one hour at -10°C, it is cooled to -70°C and mixed drop by drop with 590 ml (590 mmol) of LiAlH4 solution (1 M in THF). After stirring is continued for one hour at -70°C, it is allowed to reach -40°C. 600 ml of 50% acetic acid is added. It is stirred overnight at room temperature. The insoluble components are suctioned off, and the filtrate is concentrated by evaporation. The residue is purified on silica gel with hexane and hexane/ethyl acetate 1:1. 28.0 g of white solidifying oil accumulates. 63 Production of 2-Bromo-4-formyl-pyridine: O N Br 149 g (1714 mmol) of manganese dioxide is added in measured quantities to 28.0 g (148.9 mmol) of 2-bromo-4-hydroxymethyl-pyridine in 500 ml of dichlormethane within 6 hours. Then, stirring is continued at room temperature for 48 hours. It is suctioned off over Celite and concentrated by evaporation. 16.4 g of solidifying white oil accumulates. 2-Bromo-4-formyl-pyridine can also be produced according to THL 42, 6815 (2001) from 2-bromo-4-picoline in two stages.

Production of 2-[(6-Bromo-pyridin-3-ylmethyl)-amino]-iV-isoquinolin-3-yl-benzamide 3.46 g (13.17 mmol) of 2-amino-Ar-isoquinolin-3-yl-benzamide is introduced into 50 ml of methanol, mixed with 1.5 ml of glacial acetic acid as well as 2.45 g (13.17 mmol) of 2-bromopyridine-5-carbaldehyde and stirred for 24 hours under argon and in a Stage 2 64 moisture-free environment at room temperature. Then, it is mixed with 828 mg (13.17 mmol) of sodium cyanoborohydride and stirred for another 24 hours at room temperature. After concentration by evaporation under vacuum, the residue is taken up in dilute sodium bicarbonate solution and suctioned off. The residue that is obtained is absorptively precipitated in a little ethyl acetate and suctioned off repeatedly. The residue that is obtained in this case is chromatographed on silica gel with hexane:ethyl acetate =1:1 as an eluant. 3.27 g (57% of theory) of 2-[(6-bromo-pyridin-3-ylmethyl)-amino]-jV-isoquinolin-3-yl-benzamide is obtained. 65 Similarly produced are: 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-jV-isoquinoIin-3-yl-benzamide 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-iV-(3-trifluoromethyl-phenyl)-benzamide 66 Example B 1st Stage Production of 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-benzoic Acid Methyl Ester 6.04 g (40 mmol) of anthranilic acid methyl ester in 600 ml of methanol is mixed with 3.2 ml of acetic acid and 7.4 g (40 mmol) of 2-bromopyridine-4-carbaldehyde and stirred overnight at 40°C. 3.8 g (60 mmol) of sodium cyanoborohydride is added thereto and stirred overnight at 40°C. 3.8 g (60 mmol) of sodium cyanoborohydride is added again and stirred over the weekend at 40°C. It is mixed with water and largely concentrated by evaporation. The aqueous phase is extracted with ethyl acetate, and the combined organic phases are dried, filtered and concentrated by evaporation. The crude product is chromatographed on silica gel with a gradient that consists of hexane and hexane/ethyl acetate 1:3 and hexane/ethyl acetate 1:1 as an eluant. 10.0 g (78% of theory) of 2-[(2-bromo-pyridin-4-ylmethyl)-amino]-benzoic acid methyl ester is obtained as a colorless oil. o 67 Example C 1st Stage Production of 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-benzoic Acid .0 g (31.2 mmol) of 2-[(2-bromo-pyridin-4-ylmethyl)-amino]-benzoic acid methyl ester is dissolved in 290 ml of ethanol and mixed with 31.2 ml of 2 M sodium hydroxide solution. After having been stirred overnight at room temperature, the ethanol is drawn off, and the aqueous phase is shaken out with ethyl acetate. The aqueous phase is acidified with concentrated hydrochloric acid. The precipitate that is formed is suctioned off and dried. 5.93 g (62%) of 2-[(2-bromo-pyridin-4-ylmethyl)-amino]-benzoic acid accumulates in the form of a white solid. 68 2nd Stage Production of 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-jV-(2-methyl-2//-indazoI-6-yl)-benzamide 0.500 g (1.6 mmol) of 2-[(2-bromo-pyridin-4-ylmethyl)-amino]-benzoic acid, 0.471 g (3.2 mmol) of 2-methyl-2//-indazol-6-ylamine, 0.4 ml (3.68 mmol) ofN-methylmorpholine and 0.729 g (1.92 mmol) of 0-(7-azabenzotriazol-l-yl)-l,l,3,3-tetramethyluroniumhexafluorophosphate (HATU) in 25 ml of dimethylformamide are stirred for 16 hours at room temperature. The dimethylformamide is drawn off in an oil pump vacuum. The remaining residue is drawn off in saturated sodium bicarbonate solution. It is extracted three times with ethyl acetate, and the combined organic phases are dried, filtered and concentrated by evaporation. The residue is chromatographed on silica gel with a gradient that consists of hexane:acetone = 100:0 to 50:50 as an eluant. 0.669 g (96% of theory) of 2-[(2-bromo-pyridin-4-ylmethyl)-amino]-jV-(2-methyl-2/7-indazol-6-yl)-benzamide is obtained in the form of a beige foam. 69 Similarly produced are also the following compounds: 2-[(2-Bromo-pyridin-4-ylmethyl)-aminoj-Ar-(l-methyl-l//-indazol-6-yl)-benzamide 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-iV-(l//-indazol-6-yl)-benzamide 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-Ar-(l//-indazol-5-yl)-benzamide 70 Example D Stage 1 Production of 2-{[2-(2-Oxo-pyrrolidin-l-yI)-pyridin-4-ylmethyl]-amino}-benzoic Acid Methyl Ester 870 mg (2.78 mmol) of 2-[(2-bromo-pyridin-4-ylmethyl)-amino]-benzoic acid methyl ester, 53 mg (0.28 mmol) of copper (I) iodide, 1.126 g (5.5 mmol) of potassium phosphate and 0.26 ml (3.6 mmol) of pyrrolidin-2-one are refluxed in 15 ml of dioxane for 8 hours. After water is added, the dioxane is distilled off in a vacuum, made alkaline with about 12% ammonia solution and shaken out several times with ethyl acetate. The collected ethyl acetate phase is washed, dried, filtered and concentrated by evaporation. As a residue, 700 mg (77% of theory) of 2-{[2-(2-oxo-pyrrolidin-l-yl)-pyridin-4-ylmethyl]-amino}-benzoic acid methyl ester is obtained as a crude product, which is used without further purification in the next stage. o 71 Similarly produced are: G MW Melting Point [°C] or MS Molar Peak (m/e) s -Nu° 327.3 o x . —N NH \-J 326.4 0 JJ . —N N'Me VJ 341.4 Stage 3 Production of 2-{[2-(2-Oxo-pyrrolidin-l-yl)-pyridin-4-yimethyI]-amino}-benzoic Acid 72 700 mg (2.15 mmol) of 2-{[2-(2-oxo-pyrrolidin-l-yl)-pyridin-4-ylmethyl]-amino}-benzoic acid methyl ester is mixed in 15 ml of methanol with 2.7 ml of IN sodium hydroxide solution and refluxed for 1 hour. After the methanol is distilled off in a vacuum, it is diluted with water and shaken once with ethyl acetate. The aqueous phase is mixed with 5 ml of 1 mol citric acid solution and stirred overnight. The solid precipitation is suctioned off and very quickly dried. 600 mg of 2-{[2-(2-oxo-pyrrolidin-l-yl)-pyridin-4-ylmethyl]-amino}-benzoic acid, which is used as a crude product in the next stage, is obtained.

Similarly produced are: R2, R3, Y and Z = G G MW Melting Point [°C] or MS Molar Peak (m/e) -A- 313.3 73 G MW Melting Point [°C] or MS Molar Peak (m/e) o x . —N NH 312.3 O .-N^N-Me Y-J 326.4 74 Example E Production of 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-iV-(l-methyl-l//-indazol-5-yl)-benzamide and 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-Ar-(2-methyl-2//-indazol-5-yl)-benzamide 4.22 g (10 mmol) of 2-[(2-bromo-pyridin-4-ylmethyl)-amino]-Ar-(l//-indazol-5-yl)-benzamide is mixed in 30 ml of dimethylformamide while being cooled with ice with 3.6 g (11 mmol) of cesium carbonate and 0.68 ml (11 mmol) of methyl iodide, and it is stirred overnight at room temperature. It is then stirred into 250 ml of ice-cold water, stirring is continued for 15 minutes, and it is suctioned off. The filter cake is very quickly dried and chromatographed on silica gel with a gradient of ethyl acetate :hexane = 1:1 to 100:0 as an eluant. 1.79 g (41% of theory) of 2-[(2-bromo-pyridin-4-ylmethyl)-amino]-Ar-( 1 -methyl-1 //-indazol-5-yl)-benzamide with a melting point of 173.8°C as well as 830 mg (19% of theory) of 2-[(2-bromo-pyridin-4-ylmethyl)-amino]-iV-(2-methyl-2i/-indazol-5-yl)-benzamide with a melting point of 183.8°C are obtained.

Me / 75 Similarly produced are: 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-Ar-(l-isopropyl-l//-indazol-5-yl)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-/V-(2-isopropyl-2//-indazol-5-yl)-benzamide, 2-[(2-Bromo-pyridin-4-yImethyl)-amino]-Ar-(l-ethyl-l//-indazoI-5-yI)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-Ar-(2-ethyl-2//-indazol-5-yI)-benzamide, 2- [(2-Bromo-py ridin-4-ylmethyI)-amino] -iV-(l - [2-methoxy ethyl] -l//-indazol-5-yl)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-aniino]-./V-(2-[2-methoxyethyI]-2//-indazol-5-yl)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-A?-(l-[cyanomethyl]-l//-indazol-5-yl)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-Ar-(2-[cyanomethyl]-2//-indazol-5-yl)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-A^-(l-[2-dimethylaminoethyl]-li/-indazol-5-yl)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-yV-(2-[2-dimethylaminoethyI]-2//-indazol-5-yl)-benzamide, 76 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-/V-(l-methyl-l//-indazol-6-yl)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-Ar-(2-methyl-2//-indazoI-6-yl)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-iV-(l-isopropyl-l//-indazol-6-yl)-benzamide, 2-[(2-Bromo-pyridin-4-yImethyl)-amino]-J/V-(2-isopropyI-2//-indazol-6-yl)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-./V-(l-ethyl-l//-indazol-6-yI)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-Ar-(2-ethyl-2jF/-indazol-6-yl)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-7V-(l-[2-methoxyethyl]-l//-indazol-6-yl)-benzamide, 2-[(2-Bromo-pyridin-4-yImethyl)-amino]-iV-(2-[2-methoxyethyl]-2fr-indazol-6-yi)-benzamide, 2-[(2-Bromo-pyridin-4-ylmethyI)-amino]-AL(l-[cyanomethyl]-l//-indazol-6-yl)-benzamide and 2-[(2-Bromo-pyridin-4-yImethyl)-amino]-Ar-(2-[cyanomethyl]-2//-indazol-6-yl)-benzamide. 2-[(2-Bromo-pyridin-4-ylmethyl)-amino]-7V-(l-|2-dimethylaminoethyI]-l//-indazol-6-yI)-benzamide and 77 2-[(2-Bromo-pyridin-4-ylmethyI)-amino]-iV-(2-[2-dimethylaminoethyl]-2//- indazol-6-yl)-benzamide. 78 The sample applications below explain the biological action and the use of the compounds according to the invention without the latter being limited to the examples.

Solutions Required for the Tests Stock solutions Stock solution A: 3 mmol of ATP in water, pH 7.0 (-70°C) Stock solution B: g-33P-ATP 1 mCi/100 ^1 Stock solution C: poly-(Glu4Tyr) 10 mg/ml in water Solution for dilutions Substrate solvent: 10 mmol of DTT, 10 mmol of manganese chloride, 100 mmol of magnesium chloride Enzyme solution: 120 mmol of tris/HCl, pH 7.5,10 |liM of sodium vanadium oxide Sample Application 1 79 Inhibition of the KDR- and FLT-1 Kinase Activity in the Presence of the Compounds According to the Invention In a microliter plate (without protein binding) that tapers to a point, 10 |xl of substrate mix (10 p.1 of volume of ATP stock solution A + 25 p.Ci of g-33P-ATP (about 2.5 |xl of stock solution B) + 30 (_il of poly-(Glu4Tyr) stock solution C + 1.21 ml of substrate solvent), 10 p.1 of inhibitor solution (substances corresponding to the dilutions, 3% DMSO in substrate solvent as a control) and 10 jul of enzyme solution (11.25 fig of enzyme stock solution (KDR or FLT-1 kinase) are added at 4°C in 1.25 ml of enzyme solution (dilute). It is thoroughly mixed and incubated for 10 minutes at room temperature. Then, 10 p.1 of stop solution (250 mmol of EDTA, pH 7.0) is added, mixed, and 10 jj.1 of the solution is transferred to a P 81 phosphocellulose filter. Then, it is washed several times in 0.1 M phosphoric acid. The filter paper is dried, coated with Meltilex and measured in a microbeta counter.

The IC50 values are determined from the inhibitor concentration, which is necessary to inhibit the phosphate incorporation to 50% of the uninhibited incorporation after removal of the blank reading (EDTA-stopped reaction).

The results of the kinase inhibition IC50 in fiM are presented in the table below.

Claims (41)

80 Sample Application 2 Cytochrome P450 Inhibition The Cytochrome P450 inhibition was performed according to the publication of Crespi et al. (Anal. Biochem., 248,188-190 (1997)) with use of the baculovirus/insect cell-expressed, human Cytochrome P 450 isoenzyme (3A4). The results are presented in the following table. Example No. VEGFR II (KDR) [nM] Cytochrome P450 Isoenzyme 3A4 III M] 2.54 from 5 3.6 WO 00/27819 38 from 180 4.6 WO 00/27820 1.14 52 >30 3.24 12 14 3.30 10 5.5 6.2 41 >30 6.22 24 10 6.27 8 10 6.32 65 11 The superior action of the compounds according to the invention compared to the known compounds can be seen clearly from the result. Intellectual Property Office ef N.Z. 24 nov 2006 RECEIVED 81 What we claim is:

1. A compound of general formula I W O N / \ R3 (I). Y in which X stands for CH or N, W stands for hydrogen or fluorine, A, B, D, E and Q, in each case independently of one another, stand for a nitrogen or carbon atom, whereby at least one of A, B, D, E and Q is a nitrogen atom, R1 stands for aryl or heteroaryl, which optionally can be substituted in one or more places in the same way or differently with halogen, hydroxy, C1-C12-alkyl, C3-C6-cycloalkyl, C3-C6-alkenyl, C2-C6-alkinyl, aralkyloxy, C1-C12-alkoxy, halo-Ci-C6-alkyl, cyano-Ci-C6-alkyl or with the group =0, -S02R\ V Intellectual Property Office of N.Z. 24 NOV 2006 RECEIVED 82 or -OR5, whereby the CpC6-alkyl optionally also can be substituted with the group -OR5 or -NR9R10, Y and Z, in each case independently of one another, stand for a bond or for the group =CO, =CS or =S02, R2 and R3, independently of one another, stand for hydrogen or for the group -CONR9R10, -S02R6, -COR11, -COCi-C6-alkyl, -CO-Q-Q-alkyl-R11, -NR9R10 or for Ci-C6-alkyl, C3-Cio-cycloalkyl, C3-C6-cycloalkenyl, aryl or heteroaryl that is optionally substituted in one or more places in the same way or differently with halogen, cyano, Ci-Ci2-alkyl, Ci-Ci2-alkoxy, hydroxy-Ci-C6-alkyl, halo-C i-C6-alkyl or with the group -NR7R8, -OR5, -Ci-C6-alkyl-OR5, -SR4, -SOR4 or -S02R6, or R2, R3, Y and Z together with the nitrogen atom form a 3- to 8-membered saturated or unsaturated ring, which optionally can contain additional heteroatoms in the ring and optionally can be substituted in one or more places in the same way or differently with halogen, cyano, Ci-Ci2-alkyl, Ci-Ci2-alkoxy, halo-Ci-C6-alkyl, hydroxy-Ci-C6-alkyl, or with the group =0, -OR5, -SR4, -SOR4 or -S02R6, R4 stands for Ci-Ci2-alkyl, aryl or heteroaryl, R5 stands for hydrogen, Ci-Ci2-alkyl, C3-Cio-cycloalkyl, Ci-Ci2-alkoxy, halo- Ci-Ci2-alkyl, or halo-C3-C6-cycloalkyl, R6 stands for hydrogen, Ci-Ci2-alkyl, halo-Ci-C6-alkyl, aryl or heteroaryl, or for the group -NR9R10, whereby the aryl or heteroaryl itself optionally can Intellectual Property Office of N.2. 2 <l NOV 2006 RECEIVED 83 be substituted in one or more places in the same way or differently with Ci-Ci2-alkyl, Ci-C6-alkoxy, halogen or halo-Ci-C6-alkoxy, R7 and R8, independently of one another, stand for hydrogen or Ci-Ci2-alkyl, and R9 and R10, independently of one another, stand for hydrogen, Ci-C6-alkyl, C2-C6-alkenyl, aryl, C3-Cg-cycloalkyl or for the group -CONR7R8, or for Ci-Ci2-alkyl that is optionally substituted in one or more places in the same way or differently with aryl, morpholino, hydroxy, halogen, C1-C12-alkoxy, or for the group -NR7R8, whereby the aryl itself optionally can be substituted in one or more places in the same way or differently with Ci-C6-alkoxy or halo-C i-C6-alkyl, or R9 and R10 together form a 5- to 8-membered ring that can contain additional heteroatoms, and R11 stands for Ci-Cg-alkyl, Ci-C6-alkoxy, hydroxy-Ci-C6-alkyl, hydroxy-Ci-C6-alkoxy, C3-C6-cycloalkyl, phenyl, pyridyl, biphenyl or naphthyl, whereby the phenyl itself can be substituted in one or more places in the same way or differently with Ci-C6-alkyl, or halo-Ci-C6-alkyl, or an isomer, diastereomer, tautomer or salt thereof.

2. A compound of general formula I, according to claim 1, in which X stands for CH, W stands for hydrogen, A, B, D, E and Q as a ring together stand for pyridyl, R1 stands for aryl or heteroaryl, which optionally can be substituted in one or intellectual Property Office of N.Z. IS NOV 2006 received 84 more places in the same way or differently with halogen, hydroxy, C1-C6-alkyl, c3-c6-cycloalkyl, c4-C6-alkenyl, c2-c6-alkinyl, aralkyloxy, C1-C6-alkoxy, halo-Ci-C6-alkyl, cyano-Cj-Ce-alkyl, or with the group =0, -S02R6or -OR5, whereby Ci-c6-alkyl optionally also can be substituted with the group -OR5 or -NR9R10, Y and Z, in each case independently of one another, stand for a bond, R2 and R3, independently of one another, stand for hydrogen or for the group -CONR9R10, -so2r6, -COR11, -COCi-C6-alkyl, -CO-Ci-C6-alkyl-Rn, -NR9R10 or for Ci-c6-alkyl, c3-c6-cycloalkyl, c3-c6-cycloalkenyl, aryl or heteroaryl that is optionally substituted in one or more places in the same way or differently with halogen, cyano, Ci-C6-alkyl, Ci-C6-alkoxy, hydroxy-Ci-C6-alkyl, halo-Ci-C6-alkyl or with the group -NR7R8, -OR5, -Ci-C6-alkyl-OR5, -SR4, -SOR4 or-S02R6, or R2, R3, Y and Z together with the nitrogen atom form a 3- to 8-membered saturated or unsaturated ring, which optionally can contain additional heteroatoms in the ring and optionally can be substituted in one or more places in the same way or differently with halogen, cyano, Ci-ci2-alkyl, Ci-ci2-alkoxy, halo-Ci-c6-alkyl, hydroxy-Ci-c6-alkyl or with the group =0, -OR5, -SR4, -SOR4 or -so2r6, R4 stands for Ci-C6-alkyl, aryl or heteroaryl, R5 stands for hydrogen, Ci-c6-alkyl, halo-Ci-c6-alkyl, Ci-ci2-alkoxy, c3-Cio-cycloalkyl or halo-c3-c6-cycloalkyl, Intellectual Property Office of N.Z. 2 NOV 2006 received 85 R6 stands for hydrogen, Ci-C6-aIkyl, halo-Ci-C6-aIkyl, aryl or heteroaryl, or for the group -NR9R10, whereby the aryl or heteroaryl itself optionally can be substituted in one or more places in the same way or differently with Ci-C6-alkyl, Ci-C6-alkoxy, halogen or halo-Ci-C6-alkoxy, R7 and R8, independently of one another, stand for hydrogen or Ci-C6-alkyl, R9 and R10, independently of one another, stand for hydrogen, Ci-C6-alkyl, C2-C6-alkenyl, aryl, Cs-Cg-cycloalkyl, or for the group -CONR7R8, or for C1-C6-alkyl that is optionally substituted in one or more places in the same way or differently with aryl, morpholino, hydroxy, halogen or Ci-Cn-alkoxy, or for the group -NR7R8, whereby the aryl itself optionally can be substituted in one or more places in the same way or differently with Ci-C6-alkoxy or halo-C i-Ce-alkyl, and R11 stands for Ci-C6-alkyl, Ci-C6-alkoxy, hydroxy-Ci-C6-alkyl, hydroxy-Ci-C6-alkoxy, C3-C6-cycloalkyl, phenyl, pyridyl, biphenyl or naphthyl, whereby the phenyl itself can be substituted in one or more places in the same way or differently with Ci-C6-alkyl, or halo-Ci-C6-alkyl, or an isomer, diastereomer, tautomer or salt thereof.

3. A compound of general formula I, according to claim 1 or 2, in which X stands for CH, W stands for hydrogen, A, B, D, E, and Q as a ring together stand for pyridyl, R1 stands for phenyl, quinolinyl, isoquinolinyl or indazolyl, which optionally Intellectual Properly Office of N.Z. lh NOV 2006 received 86 can be substituted in one or more places in the same way or differently with halogen, hydroxy, Ci-C6-alkyl, C2-C6-alkinyl, Ci-C6-alkoxy, halo-Ci-C6-alkyl, or cyano-Ci-C6-alkyl, whereby Ci-C6-alkyl optionally also can be substituted with the group -OR5 or -NR9R10, Y and Z, in each case independently of one another, stand for a bond, or for the group =CO, R2 and R3, independently of one another, stand for hydrogen or for the group -CONR9R10, -S02R6, -COR11, -COCi-C6-alkyl, -CO-Ci-C6-alkyl-Ru, -NR9R10 or for Ci-C6-alkyl or phenyl that is optionally substituted in one or more places in the same way or differently with the group -NR7R8 or -OR5, or R2,R3,Y and Z together with the nitrogen atom form a 3- to 8-membered saturated or unsaturated ring that optionally can contain additional heteroatoms in the ring and optionally can be substituted in one or more places in the same way or differently with halogen, cyano, Ci-Ci2-alkyl, Ci-Ci2-alkoxy, halo-Ci-C6-alkyl, hydroxy-Cl-Cg-alkyl or with the group =0, -OR5, -SR4, -SOR4 or -SO2R6, R5 stands for hydrogen or Ci-C6-alkyl, R6 stands for hydrogen, Ci-C6-alkyl, halo-Ci-C6-alkyl, phenyl, benzyl, thiophenyl, or pyridyl, whereby the phenyl, benzyl, thiophenyl and pyridyl itself optionally can be substituted in one or more places in the same way Intellectual property Office of N.Z. 21» NOV 2006 received 87 or differently with Ci-C6-alkyl, Ci-C6-alkoxy, halogen or halo-Ci-C6-alkoxy, R7 and R8, independently of one another, stand for hydrogen or Ci-C6-alkyl, R9 and R10, independently of one another, stand for hydrogen, Ci-C6-alkyl, C2-C6-alkenyl, phenyl, biphenyl, C3-Cg-cycloalkyl, naphthyl or for the group -CONR7R8 or for Ci-C6-alkyl that is optionally substituted in one or more places in the same way or differently with phenyl, morpholino, hydroxy, halogen, Ci-Ci2-alkoxy, or with the group -NR7R8, whereby the phenyl itself optionally can be substituted in one or more places in the same way or differently with Ci-C6-alkoxy or halo-Ci-C6-alkyl, and R11 stands for Ci-C6-alkyl, Ci-C6-alkoxy, hydroxy-Ci-C6-alkyl, hydroxy-Cr C6-alkoxy, C3-C6-cycloalkyl, phenyl, pyridyl, biphenyl or naphthyl, whereby the phenyl itself can be substituted in one or more places in the same way or differently with Ci-Ce-alkyl, or halo-Ci -C6-alkyl, or an isomer, diastereomer, tautomer or salt thereof.

4. A pharmaceutical agent comprising at least one compound of any one of claims 1 to 3.

5. A pharmaceutical agent according to claim 4 for use in the case of tumor or metastasis growth, psoriasis, Kaposi's sarcoma, restenosis, endometriosis, Crohn's disease, Hodgkin's disease, leukemia, arthritis, hemangioma, angiofibroma, eye diseases, renal diseases, fibrotic diseases, injuries to nerve tissue, inhibition of the reocclusion of vessels after balloon catheter treatment, vascular prosthetics or use of mechanical devices to keep vessels open, and as immunosuppressive agents, and for supporting scar-free healing, in senile keratosis and in contact dermatitis. Intellectual Property Office of N.Z. 2 S NOV 2006 d c r. p 1 v E D 88

6. A pharmaceutical agent according to claim 5, where the restenosis is stent-induced restenosis.

7. A pharmaceutical agent according to claim 5, where the arthritis is rheumatoid arthritis.

8. A pharmaceutical agent according to claim 5, where the eye disease is selected from diabetic retinopathy and neovascular glaucoma.

A pharmaceutical agent according to claim 5, where the renal disease is selected from glomerulonephritis, diabetic nephropathy, malignant nephrosclerosis, thrombic microangiopathic syndrome, transplant rejections and glomerulopathy.

10. A pharmaceutical agent according to claim 5, where the fibrotic disease is selected from cirrhosis of the liver, a mesangial cell proliferative disease and arteriosclerosis.

11. A pharmaceutical agent according to claim 5, where the mechanical device to keep vessels open is a stent.

12. A pharmaceutical agent according to claim 5 for use as a VEGFR kinase 3-inhibitor of lymphangiogenesis.

13. A compound according to any one of claims 1 to 3 and a pharmaceutical agent, according to any one of claims 4 to 12, with suitable formulations and vehicles.

14. Use of a compound according to any one of claims 1 to 3, as an inhibitor of the tyrosine kinases KDR and FLT in non-human animals. Intellectual Property Office of N.Z. 21, NOV 2006 i received 89

15. Use of a compound according to any one of claims 1 to 3, in the form of a pharmaceutical preparation for enteral, parenteral or oral administration to non-human animals.

16. Use of a compound according to any one of claims 1 to 3 in the case of tumor or metastasis growth, psoriasis, Kaposi's sarcoma, restenosis, endometriosis, Crohn's disease, Hodgkin's disease, leukemia, arthritis, hemangioma, angiofibroma, eye diseases, renal diseases, fibrotic diseases, injuries to nerve tissue, and for inhibiting the reocclusion of vessels after balloon catheter treatment, in vascular prosthetics or after mechanical devices are used to keep vessels open, and as immunosuppressive agents, and for supporting scar-free healing, and in senile keratosis and in contact dermatitis in non-human animals.

17. Use according to claim 16, where the restenosis is stent-induced restenosis.

18. Use according to claim 16, where the arthritis is rheumatoid arthritis.

19. Use according to claim 16, where the eye disease is selected from diabetic retinopathy and neovascular glaucoma.

20. Use according to claim 16, where the renal disease is selected from glomerulonephritis, diabetic nephropathy, malignant nephrosclerosis, thrombic microangiopathic syndrome, transplant rejections and glomerulopathy.

21. Use according to claim 16, where the fibrotic disease is selected from cirrhosis of the liver, a mesangial cell proliferative disease and arteriosclerosis.

22. Use according to claim 16, where the mechanical device to keep vessels open is a stent. IrrietTecruai Property Office ef N.z. 2 h NOV 2006 Irii flfUJrfG>& I \/1l 90

23. Use of a compound according to any one of claims 1 to 3, in the preparation of a composition for inhibiting the tyrosine kinases KDR and FLT.

24. Use of a compound according to any one of claims 1 to 3, in the preparation of a composition adapted for enteral, parenteral or oral administration.

25. Use of a compound according to any one of claims 1 to 3, in the preparation of a medicament in the case of tumor or metastasis growth, psoriasis, Kaposi's sarcoma, restenosis, endometriosis, Crohn's disease, Hodgkin's disease, leukemia, arthritis, hemangioma, angiofibroma, eye diseases, renal disease, fibrotic diseases, injuries to nerve tissue, and for inhibiting the reocclusion of vessels after balloon catheter treatment, in vascular prosthetics or after mechanical devices are used to keep vessels open, and as immunosuppressive agents, and for supporting scar-free healing, and in senile keratosis and in contact dermatitis.

26. Use according to claim 25, where the restenosis is stent-induced restenosis.

27. Use according to claim 25, where the arthritis is rheumatoid arthritis.

28. Use according to claim 25, where the eye disease is selected from diabetic retinopathy and neovascular glaucoma.

29. Use according to claim 25, where the renal disease is selected from glomerulonephritis, diabetic nephropathy, malignant nephrosclerosis, thrombic microangiopathic syndrome, transplant rejections and glomerulopathy.

30. Use according to claim 25, where the fibrotic disease is selected from cirrhosis of the liver, a mesangial proliferative disease and arteriosclerosis.

31. Use according to claim 25, where the mechanical device to keep vessels open is a stent. Intellectual Property Office of N.Z. 24 NOV 2006 received 91

32. A compound of general formula II, Ha or III, W O X NH (Ha) in which A, B, D, E, Q, W, X, Y, Z, R1, R2, and R3 are as defined in claim 1, and M stands for halogen, FG stands for a leaving group, and RY stands for Ci-C6-alkyl or hydrogen, as an intermediate product for the production of a compound of general formula I according to claim 1.

33. A compound according to claim 1, substantially as herein described or exemplified.

34. A pharmaceutical agent according to claim 4, subslanliaJij or exemplified. | Qffice N.Z. 2 <» NOV 2006 received 92

35. A use according to claim 14, substantially as herein described or exemplified.

36. A use according to claim 15, substantially as herein described or exemplified.

37. A use according to claim 16, substantially as herein described or exemplified.

38. A use according to claim 23, substantially as herein described or exemplified.

39. A use according to claim 24, substantially as herein described or exemplified.

40. A use according to claim 25, substantially as herein described or exemplified.

41. A compound according to claim 32, substantially as herein described or exemplified. m END OF CLAIMS Intellectual property Office of N.Z. 2\ NOV 2006