NZ513411A - Isolating a cytoplasmic fraction without impairing the viability of oocytes and embryonic cells - Google Patents

Isolating a cytoplasmic fraction without impairing the viability of oocytes and embryonic cells

Info

Publication number
NZ513411A
NZ513411A NZ513411A NZ51341100A NZ513411A NZ 513411 A NZ513411 A NZ 513411A NZ 513411 A NZ513411 A NZ 513411A NZ 51341100 A NZ51341100 A NZ 51341100A NZ 513411 A NZ513411 A NZ 513411A
Authority
NZ
New Zealand
Prior art keywords
oocytes
cytoplasmic fraction
impairing
isolating
embryonic cell
Prior art date
Application number
NZ513411A
Inventor
Robert Jansen
Boer Kylie De
Original Assignee
Sydney Ivf Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sydney Ivf Pty Ltd filed Critical Sydney Ivf Pty Ltd
Publication of NZ513411A publication Critical patent/NZ513411A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method of isolating a cytoplasmic fraction from an oocytes or an embryonic cell without impairing the capacity of the oocytes or embryonic cell to be fertilized includes the step of releasing a cytoplasmic fraction from the oocytes or embryonic cell which is about 5% of the volume of the oocytes or embryonic cell. The method can further comprise the steps of inserting a releasing means into the oocytes of embryonic cell, then drawing a cytoplasmic fraction into the releasing means and withdrawing the releasing means so that the fraction is isolated in the releasing means. The releasing means may be an injection pipette. Also described is a method for detecting a nucleotide sequence, polymorphism or mutation in the mitochondrial genome of the mitochondria located in the oocytes or embryonic cell without impairing its ability to be fertilized. This method comprises the steps of isolating a cytoplasmic fraction that includes mitochondria according to the above method and then analyzing the nucleotide sequence of the mitochondrial genome for the presence of a nucleotide sequence, polymorphism or mutation in the mitochondrial genome.
NZ513411A 1999-02-23 2000-02-23 Isolating a cytoplasmic fraction without impairing the viability of oocytes and embryonic cells NZ513411A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPP8841A AUPP884199A0 (en) 1999-02-23 1999-02-23 Female germ-line-cell mitochondrialgenome
PCT/AU2000/000125 WO2000050895A1 (en) 1999-02-23 2000-02-23 Isolating a cytoplasmic fraction without impairing the viability of oocytes and embryonic cells

Publications (1)

Publication Number Publication Date
NZ513411A true NZ513411A (en) 2003-03-28

Family

ID=3813043

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ513411A NZ513411A (en) 1999-02-23 2000-02-23 Isolating a cytoplasmic fraction without impairing the viability of oocytes and embryonic cells

Country Status (10)

Country Link
EP (1) EP1155320A1 (en)
JP (1) JP2002537782A (en)
KR (1) KR20010108249A (en)
CN (1) CN1341212A (en)
AU (1) AUPP884199A0 (en)
CA (1) CA2362486A1 (en)
HK (1) HK1043623A1 (en)
IL (1) IL144779A0 (en)
NZ (1) NZ513411A (en)
WO (1) WO2000050895A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102607908B (en) * 2012-02-27 2013-10-30 中国水产科学研究院黑龙江水产研究所 Preparation method of freshwater fish sperm samples for scanning electron microscope

Also Published As

Publication number Publication date
JP2002537782A (en) 2002-11-12
KR20010108249A (en) 2001-12-07
AUPP884199A0 (en) 1999-03-25
IL144779A0 (en) 2002-06-30
CN1341212A (en) 2002-03-20
EP1155320A1 (en) 2001-11-21
CA2362486A1 (en) 2000-08-31
HK1043623A1 (en) 2002-09-20
WO2000050895A1 (en) 2000-08-31

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