NZ325704A - Nucleotide analogs - Google Patents

Abstract

Nucleotide phosphonate esters characterized by the presence of an ester linked group which is bonded to the phosphorus atom of phosphonate nucleotide analogs are disclosed. The analogs comprise an ester bond that is hydrolyzed in vivo to yield a corresponding phosphonate nucleotide analog. Methods and intermediates for their synthesis and use are described.

Description

New Zealand Paient Spedficaiion for Paient Number 325704 NUCLEOTIDE ANALOGS Background of the Invention 10 The present invention relates to nucleotide analog esters, their pharmaceutically acceptable acid addition salts, processes for their production, and to their use Compounds related to the nucleotide analogs of the present invention may be found in U S Patent Numbers 5,043,339, 5,108,994 and 5,166,198, EP 206 15 459, EP 253 412, EP 269 947, EP 270 885, EP 319 228, EP 343 133, EP 398 231, EP 404 296, EP 465 297, EP 468 119, EP 468 866, EP 479 640, EP 481 214, EP 494 370, EP 531 597, PCT/GB91/01171, PCT/US92/01020, PCT/US92/05208, WO 91/19721, Bronson et al, Bioorg Medicinal Chem Lett (1992) 2 685-690, Bronson et al, T Med Chem, (1989) 221457-1463, Bronson et al, Nucleotide Analogs as Antiviral 20 Agents. ACS Symposium Series 401, J C Martin, Ed , p 72-87, American Chemical Society, Washington, DC (1989), Colla, et al, T Med Chem (1983) 2£ 602-604, Curley, et al, Antiviral Res (1990) li 345-356, De Clercq, et al, Nature. (1986) 323 464-467, Farrow, et al, T Med Chem (1990) 22 1400-1406, Farquhar, et al, 1_ Pharm Sci (1983) 71324-325, Freed, et al, Biochem Pharmacol (1989) 1£ 3193-3198, 25 Freeman, et al, T Med Chem (1992) 25 3192-3196, Gabnelsen, B , et al, Antiviral Res Suppl I (1992) 1Z149, Gumport, et al, Proc Natl Acad Sci (1971) 2559-2563. Juodka, et al, Coll Czech Chem Commun (1974) 2£ 963-968, Kim, et al, Bioorg Medicinal Chem Lett (1992) 2 367-370, Kim, et al, Tet Lett (1992) 22 25-28, Kim, et al, T Med Chem (1990) 221207-1213, Kumar, et al, T Med Chem (1990) 22 2368-30 2375, McGuigan, et al, Antiviral Chem Chemother (1993) 4 97-101, McGuigan, et al, Antiviral Res (1991) 15 255-263, Rosenberg, et al, Coll Czec on page 1 at lines 35-367, Rosenberg, et al, Coll Czech Chem Commun (1988) 52. 2792-2800, Rosenberg, et al, Coll Czech Chem Commun (1988) 52 2801-2808, Starrett, et al, Antiviral Res (1992) I£ 267-273, Yu, et al. T Med Chem (1992) 25 2958-2969, 35 Wolff-Kugel, et al, Tet Lett (1991) 22 6341-6344 WO 95/07920 describes certain alkylsalicyl esters of cyclic HPMPC A characteristic of nucleotide analogs or nucleotides having a phosphonate or a phosphate group is the presence of one or two negative charges 1 AMENDED SHEET associated with the phosphorus group at physiologic pH Workers believe the charge associated with moieties such as phosphate or phosphonate groups generally limit oral bioavailability by limiting passive diffusion through the intestine membrane (Liebman, et al, T Biol Chem . (1955) 216 823-830, Roll, et 5 al, T Biol Chem. (1956) 220 439-444, Snvastava, et al, Bioorg Chem (1984) 12 118-129, Palu, et al, Antiviral Res (1991) 115-119, Sastry, et al, Mol Pharmacol (1992) 41441-445) Workers often administer these compounds parenterally to obtain therapeutic serum or intracellular levels C Objects of the Invention The invention provides one or more compounds that meet one or more of the following objects A principal object of the invention is to provide phosphonate esters of cyclic nucleotide analogs These compounds can have improved 15 pharmacokinetic or pharmacodynamic properties compared to the parent nucleotide analog that lacks the ester moiety The nucleotide phosphonate esters (hereafter "NPEs") usually have increased oral bioavailability in humans and animals Another object is to provide NPEs with reduced toxicity and/or increased 20 potency compared to the parent compound lacking the ester moiety The invention provides NPEs that increase the therapeutic window for the parent nucleotide phosphonate by supplying it in a form that is less toxic m vivo while substantially retaining the parent nucleotide phosphonate antiviral activity Another object is to provide NPEs that deestenfy while permitting 25 subsequent biochemical or enzymatic conversion of the cyclic nucleotide analog to a desired ring opened derivative Other objects are to provide intermediates m the synthesis of the NPEs and to provide improved methods for NPE synthesis Summary of the Invention In a principal embodiment, nucleotide analogs comprising a phosphonate moiety and a hydrolyzable ester linkage at the phosphonate phosphorus atom are objects of this invention The NPEs hydrolyze m vivo to the corresponding nucleotide phosphonate and are thus precursors of the corresponding nucleotide 35 phosphonate Prmcipal invention embodiments are NPEs of formula (1) and a salt, solvate or racemate thereof 2 AMENDED SHEET WO 97/24361 PCT/US96/20226 B (1) where B is a protected or unprotected heterocyclic base, R is hydrogen (H), alkyl, O-alkyl, -CHO, -C(0)0R2, -C(0)R2, -C(0)N(R3)2 or -S(0)2N(R3)2, each R1 is independently hydrogen, cyano (CN), nitro (NO2), halogen, alkyl, O-alkyl, -C(OPR3, -C(0)R3, -S(0)20H, -N(R3)2, -CHO or -OH, and each R2 and each R3 are independently hydrogen, alkyl, phenyl, alkyl substituted phenyl, -CH2C6H5 or -CH2CH2C6H5 Atoms designated with (*) mean that the designated atom has constituents 10 that are in the (R), (S) or (RS) configuration Invention embodiments include stereochemically enriched or resolved or substantially resolved NPEs and the carbon or the phosphorus atom chiral center * is as the (R) or (S) enantiomer Other embodiments include formula (1) NPEs where R is -C(0)0R2, R1 15 and B have the meanings defined above, and R2 is n-butyl or an alkyl group having more than 4 carbon atoms, e g, an alkyl group having 5, 6, 7, 8, 9, 10,11 or 12 carbon atoms Other embodiments include methods comprising orally administering to a subject an antivirally effective dose of an invention NPE 20 Other embodiments include methods to prepare the invention stereochemically enriched NPEs at the phosphorus atom chiral center * Embodiments of the NPEs and their intermediates of the instant invention include the corresponding salts, which may be base salts of the phosphoruc acid moiety, an acid addition salt of the base, their zwittenonic 25 forms, their unionized forms, and/or anv organic or aqueous solvate Salts will typically be suitable for pharmaceutical or veterinary purposes, but also include salts that are useful for other purposes, e g, NPE synthesis Pharmaceutical^ acceptable non-toxic salts of these compounds may include those derived by combination of appropriate cations such as alkali and alkaline earth metal 10ns 30 or ammonium and quaternary amino 10ns with the acid anion moiety of the phosphoruc acid group NPEs also include acid addition salts of certain organic 3 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 and inorganic acids that result from reaction with basic centers of the purine, specifically guanine, or pynnudine base The compounds of the present invention can comprise diastereomenc mixtures that may exist Compounds of formula (1) will usually be in the S 5 configuration at the chiral carbon and will be in the R, S or R5 configuration at the chiral phosphorus Both chiral centers are designated * herein While one can separate the diastereomenc mixtures into their individual isomers through well-known techniques such as, for example, HPLC, m most instances, for compounds of the present invention, one can usually synthesize the preferred 10 optical isomer by means of stereospecific reactions (usually at the phosphorus atom) and/or by using the appropriate stereoisomer of the desired starting material (usually at tht carbon atom) NPE embodiments include NPEs labeled with a detectable tag such as a radioisotope (including 32P, 35S, 14C, 3H, 12SI), a fluorescent moiety, an enzyme 15 (including peroxidase, phosphatase) or the like Embodiments also include immunogens for raising antibodies that are capable of binding to the invention NPEs and/or their dihydroxy phosphonate hydrolysis products Detailed Description of the Invention As used herein, alkyl means linear, branched or cyclic saturated hydrocarbon moieties and includes all positional isomers, e g, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, t-butyl, n-pentyl, isopentyl, neopentyl, n-hexyl, 1-methylpentyl, 2-methylpentyl, 1-ethylbutyl, 2-ethylbutyl, 3-ethylbutyl, 25 cyclohexyl, n-heptyl, n-octyl, 2-ethylhexyl, n-nonyl, n-decyl and the like, unless the disclosure or context specifies otherwise Alkyl, when used, e g , to define 'the scope of formula (1) compounds can comprise about 1-20 carbon atoms and generally will comprise about 2-16 or about 2-12 carbon atoms Halogen means F (fluorine), CI (chlorine), Br (bromine) and I (iodine) Substantially resolved means a compound enriched for a particular chiral species A substantially resolved compound, such as a NPE substantially resolved at the chiral phosphorus atom, will often consist of at least about 65% or at least about 80% or at least about 90% of the (R) or (S) isomer A resolved compound, such as a formula (1) NPE resolved at a chiral carbon atom means 35 that the compound exists as 100% of a given configuration at the chiral atom, within the detection limits of conventional measurements 4 Printed from Mimosa 13 07 15 Racemate or racemic mixture means any mixture containing 2 or more optical isomers of a given compound For example, a formula (1) NPE racemate containing two positional or stereochemical isomers linked to or at the phosphorus atom may contain exactly 50% of each isomer or it may contain any 5 amount above 50% and below 100% ot a particular isomer The MPEs optionally exclude the following compounds from formula (1) 1 R and all R1 together are not one C1-12 O-alkyl group and 3 hydrogen atoms and R and all R1 together are not two C1-12 O-alkyl groups and 2 hydrogen atoms, 2 R and all R1 together are not one O-ethyl group and one OH group when the remaining R and R1 groups are hydrogen, 3 when R is hydrogen, ail R1 together are not one cyano group and two hydrogens and when R is hydrogen, all R1 together are not two cyano groups and one hydrogen, 4 when R is hydrogen, all R1 together are not three halogen atoms and when R is hydrogen, all R1 together are not two halogen atoms and one hydrogen atom and when R is hydrogen, all R1 together are not one halogen atom and two hydrogen atoms, 5 R and all R1 together are not one -C(0)0-Ci4-alkyl group and three hydrogen atoms, 6 R and all R1 together are not two -C(0)0-C2Hs groups and two hydrogen atoms, 7 all R1 together are not one or two nitro groups when R and the rem?imng R1 are all hydrogen, 8 all R1 together are not one or two hydroxyl groups when R and the remaining Rl are all hydrogen, 9 R and all R1 together are not one C1-6 alkyl group and three hydrogen atoms, 10 R and all R1 together are not four hydrogen atoms, 11 all R1 together are not -N(CH3)2 and two hydrogen atoms when R is a hydrogen atom, and 12 R and all R1 together are not one -C(0)0-C2Hs group, one hydroxyl and two hydrogen atoms We refer to the NPE compounds excluded from the formula (1) NPEs by provisos 1-12 as the "excluded NPEs " One exemplary group of formula (1) NPEs optionally comprises species where the NPEs differ from the excluded NPEs by Printed from Mimosa 13 07 15 the presence in the excluded NPEs of additional 1, 2,3, 4, 5 or 6 ethyl or ethylene groups, i e , the excluded NPEs include not only the enumerated exclusions, but as well an added methyl or methylene group inserted at any site in tne excluded compounds Formula (1) NPEs optionally comprise species where all R1 are hydrogen, species where two R1 are hydrogen and one R1 is any single defined R1 group and species where more than one R1 is a defined R1 group other than hydrogen Formula (1) NPEs optionally also comprise species where *wo R1 are hydrogen and one R1 is any single defined R1 group other than hydrogen, e g, two R1 are H 10 and the remaining R1 is -C(0)0R2 where R2 is alkyl having 3, 4, 5, 6, 7 or 8 carbon atoms or R2 is -C6H}-p-C(CH3)3 Exemplary formula (1) compounds can comprise species where R is -C(0)0R2, R2 is alkyl having l-< carbon atoms and at least 1 R1 is not hydrogen or methyl Exemplary formula (1) compounds can comprise species where R is -C(0)0R2, R2 is alkyl having 5, 6, 7, 8, 9 or 10 carbon atoms and 15 two R1 are hydrogen and the remaining R1 is, halogen, cyano, nitro, hydroxyl or -N(R3)2 where each R3 is the same or different Exemplary formula (1) compounds can comprise species where R is hydrogen, two R1 are hydrogen and the remaining R* is, alkyl having 1, 2, 3, 4, 5, 6, 7, 8, 9, 10,11 or 12 carbon atoms, or the remaining R1 is O-alkyl, -C(0)0R3, -C(0)R3, -S{0)20H, -CHO, halogen, cyano, 20 rutro, hydroxyl or -N(R3)2 where each R3 is the same or different Exemplary formula (1) compounds can comprise species where R is hydrogen, one R1 is hydrogen and the remaining R1 are independently, alkyl having 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12 carbon atoms, or the remaining R1 are independently O-alkyl, -C(0)0R3, -C(0)R3, -S(0)20H, -CHO, halogen, cyano, rutro, hydroxyl or -N(R3)2 25 where each R3 is the same or different Exemplary embodiments of R when R is alkyl or O-alkyl comprise alkyl or O-alkyl groups having 1, 2, 3,4,5, 6, 7, 8,9,10, 11,12,13,14,15,16,17,18,19,20 or more carbon atoms R can comprise methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, t-butyl, n-pentyl, isopentyl, neopentyl, n-hexyl, 1-methylpentyl, 2-30 methylpentyl, 1-ethylbutyl, 2-ethylbutyI, 3-ethylbutyl, cyclohexyl, n-heptyl, 2- ethylpentyl, n-octyl, 1-ethylhexyl, 2-ethylhexyl, 3-ethylhexyl, n-nonyl, n-decyl and the like when R is alkyl or -O-alkyl When R contains R2,1 e, R is -C(0)0R2 or -C(0)R2, and R2 is alkyl, R2 can comprise the alkyl groups having 1,2,3, 4, 5, 6, 7, 8, 9,10,11,12,13,14,15,16,17,18,19, 20 or more carbon atoms R2 can comprise 35 alkyl having 1-12,3-6, 3-10, 5-10, 6-12 or 6-10 carbon atoms R2 can comprise methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, t-butyl, n-pentyl, isopentyl, neopentyl, n-hexyl, 1-methylpentyl, 2-methylpentyl, 1-ethylbutyl, 2-ethylbutyl, 3- 6 Printed from Mimosa 13 07 15 PCT/U&96/20226 ethylbutyl, cyclohexyl, n-heptyl, 2-ethylpentyl, n-octyl, cyclooctyl, 1-ethylhexyl, 2-ethylhexyl, 3-ethylhexyl, n-nonyl, n-decyl and the like Embodiments of R1 and R3 when they are alkyl or O-alkyl can comprise alkyl groups having 1, 2, 3, 4, 5, 6, 7, 8, 9, 10,11,12,13,14, 15,16,17, 18,19, 20 or 5 more C atoms R1 or R3 can comprise alkyl having 1-12, 3-6, 3-10, 5-10, 6-12, 6-10, 2-10, 3-10,4-10, 2-6,3-6 or 4-6 carbon atoms When R is -C(0)0R2, R2 is alkyl having 1-4 carbon atoms, and one or more R1 is alkyl, R1 can comprise alkyl having 2-10,3-10,4-10, 2-6, 3-6 or 4-6 carbon atoms R1 can comprise methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, t-butyl, n-pentyl, isopentyl, 10 neopentyl, n-hexyl, 1-methylpentyl, 2-methylpentyl, 1-ethylbutyl, 2-ethylbutyl, 3-ethylbutyl, cyclohexyl, n-heptyl, 2-ethylpentyl, n-octyl, 1-ethylhexyl, 2-ethylhexyl, 3 ethylhexyl, n-nonyl, n-decyl and the like when R1 is alkyl cr -O-alkyl R3 can comprise methyl, ethyi, n-propyl, isopropyl, n-butyl, sec-butyl, t-butyl, n-pentyl, isopentyl, neopentyl, n-hexyl, 1-methylpentyl, 2-methylpentyl, 1-ethylbutyl, 2-15 ethylbutyl, 3-ethylbutyl, cyclohexyl, n-heptyl, 2-ethylpentyl, n-octyl, cyclooctyl, 1-ethylhexyl, 2-ethylhexyl, 3-ethylhexyl, n-nonyl, n-decyl and the like when R3 is alkyl When two R3 are present on a given substituent, e g , -C(0)N(R3)2 or -N(R3)2, each R3 is the same or different Embodiments of such compounds 20 include species where one R3 is hydrogen and the other R3 is a 1, 2, 3, 4, 5 or 6 carbon alkyl group Embodiments of such compounds also include species where both R3 are the same and can both be hydrogen or a 1, 2, 3,4,5 or 6 carbon alkyl group R2 and R3 include alkyl substituted phenyl and this can comprise para-25 alkyl substituted phenyl where the alkyl group consists of 3, 4, 5 or 6 carbon atoms These alkyl groups include isopropyl, sec-butyl, t-butyl, isopentyl, neopentyl, 2-ethylbutyl and the like Embodiments of formula (1) include NPEs stereochemically resolved or enriched as the (S) or (R) eriantiomer at the carbon atom chiral center * 30 Embodiments of formula (1) include NPEs stereochemically resolved or enriched as the (S) or (R) enantiomer at the phosphorus atom chiral center * NPEs enriched at the phosphorus atom chiral center *, consists of 55% to about 99 9% of the (S) or (R) enantiomer The formula (1) NPEs undergo a multistep chemical alteration that results 35 in the biochemical or enzymatic formation of the acyclic nucleotide phosphonate parental compound For example, the NPEs of cHPMPC (l-[((S)-2-hydroxy-2-oxo-l,4,2-dioxaphosphorinan-5-yl)methyl]cytosine, cyclic HPMPC, formula (3) below) 7 Printed from Mimosa 13 07 15 are substantially first converted to the ring opened form, (S)-HPMPC (formula (4) below) as shown Rx is the residue of a formula (1) NPE ester moiety in the formula (2) structure fhe inventors believe that the final step, conversion of (3) to (4), is an intracellular event B B = cytosin-l-yl O. (2) "1 ORA B (3) ° XOH B O O, 'OH "OH (4) OH One can synthesize formula (1) NPEs where R comprises an amide as follows OH O OH O f\^OC H3 HN(R3)2 U„, — N(R3)2 One can conduct the reaction without solvents or using ethers (diethyl ether and the like) or other solvents (N-methylpyrolidinone) One can then couple compounds of structure (B) with cHPMPC or other cyclic HPMP compounds as described below One can synthesize formula (1) NPEs with R comprising a sulfonyl or sulfonamide as follows (D) 8 Printed from Mimosa 13 07 15 (D) <K) NaNO, HC1 H20 OH One can also convert (C) to (D) using PCI3 or SOCI2 One can couple compounds of structure (F) and (H) with cHPMPC or other cyclic HPMP compounds as described below One synthesizes formula (1) NPEs with R comprising a keto group as follows OH CHO R MgBr ether (I) — ^ 0) (K) One converts (J) to (K) by oxidation using a suitable reagent such as pyndimurn chlorochromate (PCC) The reactions are accomplished using a suitable ether such as diethyl or di-n-propyl ether One can also prepare compounds of structure (K) by the following reaction Pnnted from Mimosa 13 07 15 OH C(0)0CH3 (L) R2MgBr (K) One can couple structure (K) compounds with cHPMPC or other cyclic HPMP compounds as described below The synthesis schemes for (K) can use R3 instead of R2 if desired The compounds of this invention comprise any naturally occurring hetero cycle found in nucleic aads, nucleotides or nucleosides, or analogs thereof The radicals of such heterocyclic bases, designated herein as B, are generally the purine, pyrimidine or related heterocydes shown in formulas (a) - (d) wherein R6 is NH2, NHR7, NHR8, N=CHN(R7)2 or N=C(CH3)N(R7)2, R7 is Ci - Cf, alkyl, R8 is a protecting group, each R9 is independently N or CH, R10 is OH, NH2, NHR7 or NHR8, R11 is H, NH2/ NHR7 or NHR8, and R12 is NH or CH2 B includes both protected and unprotected forms of the heterocyclic bases Protecting groups for exocyclic amines and other groups are known (T W Greene et al, eds , Protective Groups in Organic Synthesis (1991), Wiley, 2nd ed ) Printed from Mimosa 13 07 15 and include N-benzoyl, isobutyryl, 4,4'-dimethoxytntyl (DMT) and the like The selection of a protecting group will be apparent to the ordinary artisan and will depend on the nature of the labile group and the chemistry that the protecting group may encounter, e g, acidic, basic, oxidative, reductive or other conditions 5 Typically, B is a 9-purinyl residue selected from guanyl, 3-deazaguanyl, 1- deazaguanyl, 8-azaguanyl, 7-deazaguanyl, adenyl, 3-deazaadenyl, 1-dezazadenyl, 8-azaadenyl, 7-deazaadenyl, 2,6-diaminopurinyl, 2-ammopurinyl, 6-chloro-2-aminopunnyl and 6-thio-2-aminopurinyl, or a B is a 1-pyrimidmyl residue selected from cytosinyl, S-halocytosinyl, 6-azacytosinyl and 5-(Cj-C3-10 alkyl)cytosinyl such as 5-methylcytosinyl A description of exemplary NPEs of formula (1) where B is cytosin-l-yl and two R1 are hydrogen is as follows Table 1 shows NPEs having specific R and R1 substituents linked to formula (1) structures where two R1 are both hydrogen The designations A3, A4, A5, A6, A7 and A8 mean an alkyl group having 3, 4, 5, 15 6, 7 and 8 carbon atoms respectively These designations include all positional isomers of these alkyl groups, e g , linear, branched and cyclic isomers TABI E1 R R1 1 -H 1 -H 2 -COOCH2CH3 2 -CH3 3 -COOA3 3 -CH2CH3 4 -COOA4 4 -A3 -COOA5 -A4 6 -COOA6 6 -A5 7 -COOA7 7 -A6 8 -COOA8 8 -A8 9 -COOA9 9 -OCH2CH3 -COOAIO -OA3 11 -COOA12 11 -OA4 12 -COOA14 12 -OA6 13 CH3 13 -OAS 14 -CH2CH3 14 -CI -A3 -F 16 -A4 16 -COOCH3 17 -A5 17 -COOCH2CH3 18 -A6 18 -COOA3 19 -A7 19 -COOA4 -A8 -COOA5 40 21 -OCH3 21 -COOA6 22 -OCH2CH3 22 -COOC6H5 11 Printed from Mimosa 13 0715 PCT7US96/20226 23 -OA3 23 -COOC<sH5-p-A3 24 -OA4 24 -COOC6H5-P-A4 -OA5 -COOC6H4-o,o-(CH3)2 26 -OA6 26 -COOC6H4-o,o-(CH2CH3)2 27 -OA7 27 -COOC6H4-o/o-(A3)2 28 -OA8 28 -COOC6H4-0-A3 29 -COCH3 29 -COOC6H4-0-A4 -COCH2CH3 -CCXX:6H4-o-A5 31 -COA3 31 -COOC6H4-0-A6 32 -COA4 32 -COCH3 33 -COA5 33 -COCH2CH3 34 -COA6 34 -COA3 -COA7 -COA4 36 -COA8 36 -COA6 37 -C(0)NH2 37 -Br 38 -C(0)N(CH3)2 38 -NO2 39 -C(0)N(CH2CH3)2 39 -CN 40 -C(0)N(A4)2 40 -CHO 41 -C(0)NH(CH3) 41 -S(0)2OH 42 -C(0)NH(CH2CH3) 42 -N(CH3)2 43 -C(0)NH(A4) 43 -N(CH2CH3)2 44 -C(0)NH(A6) 44 -OH 45 -S(0)2NH2 46 -S(0)2N(CH3)2 47 -S(0)2N(CH2CH3)2 48 -S(0)2N(A4)2 49 -S(0)2NH(CH2CH3) 50 -S(0)2NH(A4) 51 -S(0)2NH(A6) 52 -S(0)2NH(A8) 53 -COOH 54 -CHO Table 1 assigns a number to each list J R and R1 substituent The convention R R1 X designates individual formula (1) compounds and the 35 number assigned to a listed R or R1 structure corresponds to its assigned structure Formula (1) links R1 to the aromatic ring at X which designates the position 3, 4, 5 or 6 shown in the formula (1) structure below For compounds that contain two alkyl groups having the same designation, e g, A5 at both R and Rl, one can independently choose each alkyl group Tnus, a formula (1) 40 compound designated 4 19 5 has the structure 12 Printed from Mimosa 13 07 15 PCT US96/20226 COOA4 4 B COOA4 where B is cytosin-l-yl and each A4 is independently an alkyl group containing 4 carbon atoms, e g, n-butyl, sec-butyl, t-butyl, etc The compound designated 5 22 15 6 has the structure where B is cytosin-l-yl Exemplary compounds are 1 13, 2 1 3, 3 1 3, 4 13, 5 13, 6 1 3, 7 1 3,8 1 3, 9 1 3,10 1 3, 11 1 3,12 1 3,13 1 3, 14 1 3,15 1 3,16 1 3,17 1 3,18 13, 19 1 3,20 1 3, 21 1 3, 22 1 3, 23 13, 24 1 3, 25 1 3, 26 1 3,27 1 3, 28 1 3, 29 1 3,30 1 3, 10 31 1 3,32 1 3,33 1 3, 34 1 3, 35 1 3,36 1 3,37 1 3,38 1 3,39 1 3, 40 1 3, 41 1 3,42 1 3, 43 1 3,44 1 3, 45 1 3, 46 1 3, 47 1 3,48 1 3,49 1 3,50 1 3,51 1 3 ,521 3,53 1 3,54 1 3, I 2 3,223,323,4 23,5 23,6 23,723,823,923,1023, 11 23,1223,1323,1423, 15 2 3,16 2 3,17 2 3,18 2 3, 19 2 3, 20 2 3,21 2 3,22 2 3, 23 2 3, 24 2 3, 25 2 3,26 2 3, 27 2 3,28 2 3,29 2 3,30 2 3,31 23,32 23,3323,3423,3523,3623,3722, J823, 39 2 3,40 2 3, 41 2 3,42 2 3, 43 2 3,44 2 3,45 2 3,46 2 3,47 2 3,48 2 3, 49 2 3, 50 2 3, 51 2 3 ,52 2 3, 53 2 3,54 2 3,1 3 3,2 3 3,3 3 3, 4 3 3,5 3 3, 6 3 3,7 3 3,8 3 3, 9 3 3,10 3 3, II 3 3,12 3 3,13 3 3,14 3 3,15 3 3,16 3 3,17 3 3,18 3 3,19 3 3,20 3 3, 21 3 3, 22 3 3, 23 3 3,24 3 3, 25 3 3,26 3 3, 27 3 3, 28 3 3, 29 3 3,30 3 3,31 3 3,32 3 3, 33 3 3,34 3 3, 35 3 3,36 3 3, 37 3 3,38 3 3, 39 3 3,40 3 3,41 3 3,42 3 3,43 3 3,44 3 3, 45 3 3, 46 3 3, 47 3 3,48 3 3, 49 3 3,50 3 3, 51 3 3 , 52 3 3,53 3 3,54 3 3, 1 4 3,2 4 3, 3 4 3,4 4 3,5 4 3, 6 4 3, 7 4 3, 8 4 3,9 4 3,10 4 3,11 4 3,12 4 3,13 4 3,14 4 3,15 4 3,16 4 3, 17 4 3,18 4 3, 19 4 3,20 4 3, 21 4 3,22 4 3, 23 4 3, 24 4 3, 25 4 3,26 4 3, 27 4 3,28 4 3, 29 4 3,30 4 3, 31 4 3,32 4 3, 33 4 3,34 4 3, 35 4 3,36 4 3,37 4 3, 38 4 3,39 4 3,40 4 3, 41 4 3, 42 4 3, 43 4 3,44 4 3, 45 4 3,46 4 3, 47 4 3,48 4 3,49 4 3,50 4 3,51 4 3 , 52 4 3,53 4 3,54 4 3, 25 1 5 3, 2 5 3, 3 5 3,4 5 3,5 5 3,6 5 3, 7 5 3, 8 5 3,9 5 3,10 5 3,11 5 3,12 5 3,13 5 3,14 5 3, 15 5 3,16 5 3,17 53,18 5 3, 19 5 3, 20 5 3, 21 5 3, 22 5 3,23 5 3,24 5 3, 25 5 3, 26 5 3, 27 5 3,28 5 3, 29 5 3,30 5 3, 31 5 3,32 5 3,33 5 3,34 5 3,35 5 3,36 5 3, 37 5 3,38 5 3, OCH2CH3 F 13 Printed from Mimosa 13 07 15 39 5 3, 40 5 3, 415 3,42 5 3,43 5 3,44 5 3,45 5 3,46 5 3,47 5 3,48 5 3,49 5 3, 50 5 3, 51 5 3 ,52 5 3,53 5 3, 54 5 3,1 6 3, 2 6 3, 3 6 3, 4 6 3, 5 6 3, 6 6 3, 7 6 3, 8 6 3,9 6 3,10 6 3, 11 6 3,12 6 3,13 6 3,14 6 3,15 6 3, 16 6 3,17 6 3, 18 6 3,19 6 3, 20 6 3,21 6 3, 22 6 3, 23 6 3, 24 6 3, 25 6 3, 26 6 3, 27 6 3, 28 6 3, 29 6 3,30 6 3,31 6 3,32 6 3,33 6 3, 34 6 3, 5 35 6 3,36 6 3,37 6 3,38 6 3,39 6 3,40 6 3,41 6 3,42 6 3,43 6 3,44 6 3,45 6 3,46 6 3, 47 6 3,48 6 3,49 6 3,50 6 3,51 6 3 ,52 6 3,53 6 3,54 6 3,1 7 3,2 7 3,3 7 3, 4 7 3,5 7 3, 6 7 3, 7 7 3, 8 7 3, 9 7 3,10 73,11 7 3, 12 7 3,13 7 3,14 7 3,1^ 7 3,16 7 3,17 7 3,18 7 3, 19 7 3, 20 7 3, 21 7 3,22 7 3,23 7 3, 24 7 3,25 7 3, 26 7 3,27 7 3, 28 7 3,29 7 3, 30 7 3, 31 7 3,32 7 3,33 7 3, 34 7 3,35 7 3, 36 7 3,37 7 3,38 7 3,39 7 3,40 7 3,41 7 3, 42 7 3, 10 43 7 3,44 7 3,45 7 3,46 7 3,47 7 3, 48 7 3,49 7 3,50 7 3,517 3,52 7 3,53 7 3,54 7 3, I 8 3,2 8 3,3 8 3,4 8 3,5 8 3, 6 8 3, 7 8 3,8 8 3,9 8 3,10 8 3,11 8 3,12 8 3,13 8 3,14 8 3, 8 3,16 8 3,17 8 3,18 8 3,19 8 3, 20 8 3, 21 8 3, 22 8 3,23 8 3, 24 8 3,25 8 3, 26 8 3, 27 8 3, 28 8 3, 29 8 3, 30 8 3,31 8 3, 32 8 3,33 8 3, 34 8 3,35 8 3,36 8 3,37 8 3, 38 8 3, 39 8 3, 40 8 3, 41 8 3, 42 8 3,43 8 3, 44 8 3,45 8 3, 46 8 3,47 8 3,48 8 3,49 8 3, 50 8 3, 51 8 3 , 52 8 3, 53 8 3,54 8 3,19 3, 2 9 3,3 9 3,4 9 3, 5 9 3, 6 9 3, 7 9 3, 8 9 3,9 9 3,10 9 3, II 9 3,12 9 3,13 9 3,14 9 3,15 9 3,16 9 3,17 9 3,18 9 3,19 9 3, 20 9 3,21 9 3, 22 9 3, 23 9 3, 24 9 3,25 9 3, 26 9 3,27 9 3, 28 9 3, 29 9 3,30 9 3,31 9 3,32 9 3,33 9 3, 34 9 3, 9 3,36 9 3,37 9 3,38 9 3,39 9 3, 40 9 3,41 9 3,42 9 3,43 9 3, 44 9 3,45 9 3, 46 9 3, 47 9 3,48 9 3,49 9 3, 50 9 3,51 9 3 ,52 9 3, 53 9 3, 54 9 3,1 10 3, 2 10 3,3 10 3, 4 10 3, 5 10.3, 6 10 3,7 10 3, 8 10 3,9 10 3,10 10 3,11 10 3,12 10 3,13 103,14 10 3,15 10 3, 16 10 3,17 10 3,18 10 3,19 10 3,20 10 3,21 10 3,22 10 3, 23 10 3,24 10 3, 25 10 3, 26 10 3, 2710 3,28 10 3,29 10 3,30 10 3,31 10 3, 32 10 3,33 10 3, 34 10 3, 35 10 3, 36 10 3,37 10 3,38 10 3,39 10 3,40 10 3, 41 10 3, 42 10 3,43 10 3,44 10 3, 45 10 3, 46 10 3,47 10 3,48 10 3, 49 10 3,50 10 3,51 10 3 , 52 10 3, 53 10 3, 54 10 3,1 11 3, 2 11 3, 25 3 11 3,4 11 3,5 11 3( 6 11 3,7113, 8 11 3, 9 11 3,10 11 3,11 11 3,12 11 3, 13 11 3, 14 11 3,15 113,16 11 3,17113,18 11 3,19 11 3,20 11 3,21 11 3, 22 11 3, 23 11 3, 24 11 3, 25 11 3,26 11 3,27 11 3,28 11 3,29 11 3, 30 11 3, 31 11 3, 32 11 3, 33 11 3, 34 11 3,35 113,36 11 3,37 11 3,38 11 3,39 11 3, 40 11 3,41 11 3,42 11 3, 43 11 3, 44 11 3,45 11 3,46 11 3,47 11 3,48 11 3,49 11 3, 50 11 3, 51 11 3 , 52 11 3, 53 11 3, 54 11 3,112 3, 2 12 3,3 12 3,4 12 3,5 12 3, 6 12 3,7 12 3, 8 12 3,9 12 3,10 12 3,11 12 3, 12 12 3,13 12 3,14 12 3,15 12 3,16 12 3,17 12 3,18 12 3,19 12 3, 20 12 3, 21 12 3, 22 12 3, 23 12 3,24 12 3,25 12 3,26 12 3, 27 12 3, 28 12 3, 29 12 3, 30 12 3, 31 12 3, 32 12 3, 33 12 3,34 12 3,35 12 3,36 12 3,37 12 3,38 12 3, 39 12 3, 40 12 3, 41 12 3, 42 12 3,43 12 3,44 12 3,45 12 3,46 12 3,47 12 3, 48 12 3, 49 123, 50 12 3, 51 12 3, 52 12 3, 53 12 3,54 12 3,1 13 3, 2 13 3, 3 13 3, 4 13 3,5 13 3, 6 13 3,7 133, 8 13 3,9 13 3, 10 13 3,1113 3,12 13 3,13 13 3,14 13 3,15 13 3,16 13 3,17 13 3,18 13 3,19 13 3, 20 13 3, 21 13 3,22 13 3,23 13 3,24 13 3, 25 13 3, 26 13 3, 27 13 3, 28 13 3, 29 13 3, 14 Printed from Mimosa 13 07 15 13 3, 31 13 3,32 13 3, 33 13 3, 34 13 3, 35 13 3,36 13 3, 37 13 3, 38 13 3,39 13 3, 40 13 3,41 13 3,42 13 3, 43 13 3,44 13 3, 45 13 3,46 13 3,47 13 3, 48 13 3,49 13 3, 50 13 3,51 13 3 , 52 13 3, 53 13 3, 54 13 3, 1 14 3, 2 14 3,3 14 3,4 14 3, 5 14 3, 6 14 3, 7 14 3, 8 14 3, 9 14 3,10 14 3,11 143,12 14 3,13 14 3, 14 14 3,15 14 3, 16 14 3,17 14 3, 5 18 14 3,19 14 3, 20 14 3, 21 14 3, 22 14 3, 23 14 3, 24 14 3,25 14 3, 26 14 3, 27 14 3, 28 14 3, 29 14 3,30 14 3, 31 14 3, 32 14 3, 33 14 3, 34 14 3,35 14 3,36 14 3,3714 3, 38 14 3,39 14 3,40 14 3, 41 14 3, 42 14 3,43 14 3, 44 14 3,45 14 3,46 14 3,4714 3, 48 14 3,49 14 3,50 14 3, 51 14 3 , 52 14 3, 53 14 3, 54 14 3,1 15 3, 2 15 3,3 15 3, 4 15 3, 5 15 3, 6 15 3, 7 153, 8 15 3, 9 15 3,10 15 3,11 15 3,12 15 3,13 15 3,14 15 3,15 15 3, 10 16 15 3,17 15 3,18 15 3, 19 15 3, 20 15 3, 21 15 3, 22 15 3, 23 15 3, 24 15 3,25 15 3, 26 15 3,27 15 3,28 15 3, 29 15 3,30 15 3, 31 15 3, 32 15 3, 33 15 3,34 15 3,35 15 3, 36 15 3,37 15 3,38 15 3, 39 15 3, 40 15 3, 41 15 3, 42 15 3,43 15 3, 44 15 3,45 15 3, 46 15 3,47 15 3,48 15 3, 49 15 3,50 15 3,51 15 3 ,52 15 3,53 15 3,54 15 3,1 16 3, 216 3, 3 16 3, 4 16 3,5 16 3, 6 16 3, 7 16 3, 8 16 3,9 16 3,10 16 3,11 16 3,12 16 3,13 16 3, 15 14 16 3,15 16 3,16 16 3, 17 16 3, 18 16 3, 19 16 3, 20 16 3, 21 16 3, 22 16 3,23 16 3, 24 163, 25 16 3, 26 16 3, 27 16 3, 28 16 3, 29 16 3, 30 16 3,31 16 3, 32 16 3,33 16 3, 34 16 3,35 16 3,36 16 3, 37 16 3, 38 16 3, 39 16 3, 40 16 3,41 16 3, 42 16 3,43 16 3, 44 16 3,45 16 3,46 16 3, 47 16 3, 48 16 3, 49 16 3, 50 16 3,5116 3 ,5216 3,53 16 3, 54 16 3,1 17 3,2 17 3, 3 17 3,4 173,5 17 3, 6 17 3,7 17 3,8 17 3, 9 17 3,10 17 3,1117 3, 20 12 17 3,13 17 3,14 17 3,15 17 3, 16 17 3,17 17 3,18 17 3,19 17 3, 20 17 3,21 17 3, 22 173,23 17 3,24 17 3, 25 17 3, 26 17 3, 27 17 3, 28 17 3,29 17 3,30 17 3,31 17 3, 32 17 3,33 17 3,34 17 3, 35 17 3, 36 17 3, 37 17 3, 38 17 3,39 17 3, 40 17 3,4117 3, 42 17 3,43 17 3,44 17 3, 45 17 3, 46 17 3, 47 17 3, 48 17 3,49 17 3,50 17 3,5117 3 , 52 1" 3,53 17 3,54 17 3,118 3,2 18 3, 3 183,4 18 3, 5 18 3, 6 18 3,718 3,8 18 3,9 18 3, 25 10 18 3,11 18 3,12 18 3,13 18 3, 14 18 3,15 18 3, 16 18 3,17 18 3,18 18 3,19 18 3, 20 18 3, 21 18 3, 22 18 3, 23 18 3, 24 18 3, 25 18 3, 26 18 3,27 18 3, 28 18 3,29 18 3, 30 183,31 18 3,32 18 3, 33 18 3, 34 18 3, 35 18 3, 36 18 3,37 18 3,38 18 3,39 18 3, 40 18 3,41 18 3, 42 18 3, 43 18 3, 44 18 3, 45 18 3, 46 18 3,47 18 3,48 18 3,4918 3, 50 183,51 18 3 , 52 18 3,53 18 3,54 18 3,1 19 3,2 19 3,3 19 3,4 19 3, 5 19 3,6 19 3, 30 7 19 3,8 19 3,9 19 3,10 19 3,11 19 3,12 19 3,13 19 3, 14 19 3,15 19 3, 16 19 3,17 19 3, 18 19 3,19 19 3, 20 19 3, 21 19 3, 22 19 3, 23 19 3, 24 19 3,25 19 3,26 19 3,2719 3, 28 19 3, 29 19 3,30 19 3, 31 19 3, 32 19 3, 33 19 3, 34 19 3,3519 3,36 19 3,3719 3, 38 19 3,39 19 3,40 19 3, 41 19 3, 42 19 3, 43 19 3, 44 19 3,45 19 3,46 19 3,47 19 3, 48 19 3,49 19 3,50 19 3, 51 19 3 , 52 19 3,53 19 3, 54 19 3,1 20 3, 2 20 3,3 20 3, 4 20 3, 35 5 20 3, 6 20 3,7 20 3, 8 20 3,9 20 3,10 20 3, 11 20 3,12 20 3,13 20 3,14 20 3,15 20 3, 16 20 3,17 20 3, 18 20 3,19 20 3, 20 20 3, 21 20 3, 22 20 3, 23 20 3,24 20 3,25 20 3, 26 20 3,27 20 3, 28 20 3, 29 20 3, 30 20 3, 31 20 3, 32 20 3,33 20 3,34 20 3,35 20 3, Printed from Mimosa 13 07 15 36 20 3,37 20 3, 38 20 3, 39 20 3, 40 20 3, 41 20 3, 42 20 3, 43 20 3, 44 20 3,45 20 3, 46 20 3,47 20 3, 48 20 3, 49 20 3, 50 20 3, 51 20 3 , 52 20 3, 53 20 3, 54 20 3, 1 21 3, 2 21 3, 3 21 3, 4 21 3,5 21 3, 6 21 3,7 21 3, 8 21 3, 9 21 3, 10 21 3,11 21 3,12 21 3,13 21 3, 14 21 3,15 21 3,16 21 3,17 21 3,18 21 3,19 21 3, 20 21 3,21 21 3, 22 21 3,23 21 3, 5 24 21 3,25 21 3, 26 21 3. 27 21 3, 28 21 3,29 21 3,30 21 3,31 21 3,32 21 3,33 21 3, 34 21 3,35 213, 36 21 3,37 21 3, 38 21 3, 39 21 3, 40 21 3,41 21 3,42 21 3,43 21 3, 44 21 3,45 21 3, 46 21 3, 47 21 3, 48 21 3, 49 21 3, 50 21 3,51 21 3 , 52 21 3, 53 21 3, 54 21 3,1 22 3,2 22 3,3 22 3,4 22 3,5 22 3, 6 22 3.7 22 3,8 22 3,9 22 3,10 22 3,11 22 3, 12 22 3,13 22 3,14 22 3( 15 22 3,16 22 3,17 22 3,18 22 3,19 22 3, 20 22 3,21 22 3, 10 22 22 3, 23 223, 24 22 3, 25 22 3, 26 22 3, 27 22 3, 28 22 3,29 22 3,30 22 3,31 22 3, 32 223,33 22 3, 34 22 3,35 22 3, 36 22 3,37 22 3,38 22 3,39 22 3,40 2L 3,41 72 3 42 22 3,43 22 3, 44 22 3,45 22 3, 46 22 3, 47 22 3, 48 22 3,49 22 3, 50 22 3,51 22 3 , 52 22 3,53 22 3, 54 22 3,1 23 3, 2 23 3, 3 23 3,4 23 3, 5 23 3, 6 23 3, 7 23 3,8 23 3,9 23 3, 10 23 3,11 23 3,12 23 3,13 23 3,14 23 3,15 23 3,16 23 3,17 23 3,18 23 3,19 23 3, 15 20 23 3, 21 23 3, 22 23 3, 23 23 3, 24 23 3, 25 23 3, 26 23 3,27 23 3,28 23 3,29 23 3, 30 23 3,31 23 3, 32 23 3, 33 233, 34 23 3, 35 23 3,36 23 3,37 23 3,38 23 3,39 23 3, 40 23 3,41 23 3, 42 23 3, 43 23 3, 44 23 3,45 23 3, 46 23 3,47 23 3, 48 23 3,49 23 3, 50 23 3, 51 23 3 ,52 23 3,53 23 3, 54 23 3,1 24 3,2 24 3, 3 24 3, 4 24 3, 5 24 3,6 24 3, 7 24 3,8 24 3, 9 24 3,10 24 3,11 24 3,12 24 3,13 24 3,14 24 3,15 24 3,16 24 3, 17 24 3, 20 18 24 3,19 24 3, 20 24 3, 21 24 3, 22 24 3, 23 24 3, 24 24 3,25 24 3, 26 24 3, 27 24 3, 28 24 3,29 24 3, 30 24 3, 31 24 3,32 24 3, 33 24 3, 34 24 3,35 24 3,36 24 3,37 24 3, 38 24 3,39 24 3, 40 24 3,41 24 3, 42 24 3,43 24 3, 44 24 3,45 24 3,46 24 3,47 24 3, 48 24 3,49 24 3, 50 24 3, 51 24 3 , 52 24 3,53 24 3, 54 24 3,1 25 3,2 25 3,3 25 3,4 25 3, 5 25 3, 6 25 3,7 25 3, 8 25 3,9 25 3,10 25 3,11 25 3,12 25 3,13 25 3,14 25 3,15 25 3, 25 16 25 3,17 25 3,18 25 3, 19 25 3, 20 25 3, 21 25 3, 22 25 3,23 25 3, 24 25 3, 25 25 3, 26 25 3,27 25 3, 28 25 3, 29 25 3,30 25 3,31 25 3,32 25 3,33 25 3,34 25 3,35 25 3, 36 25 3,37 25 3, 38 25 3, 39 25 3, 40 25 3, 41 25 3,42 25 3,43 25 3,44 25 3, 45 25 3, 46 25 3, 47 25 3, 48 25 3,49 25 3, 50 25 3, 51 25 3 , 52 25 3,53 25 3,54 25 3,1 26 3,2 26 3, 3 26 3,4 26 3,5 26 3, 6 26 3, 7 26 3, 8 26 3, 9 26 3,10 263,11 26 3,12 26 3,13 26 3, 30 14 26 3,15 26 3, 16 26 3,17 26 3,18 26 3,19 26 3, 20 26 3,21 26 3, 22 26 3,23 26 3, 24 26 3,25 26 3, 26 26 3, 27 26 3,28 26 3,29 26 3,30 26 3,31 26 3,32 26 3,33 26 3, 34 26 3,35 26 3, 36 26 3, 37 26 3, 38 26 3, 39 26 3, 40 26 3,41 26 3,42 26 3,43 26 3, 44 26 3, 45 26 3, 46 26 3, 47 26 3, 48 26 3, 49 26 3,50 26 3,51 26 3 , 52 26 3,53 26 3, 54 26 3,1 27 3, 2 27 3,3 27 3,4 27 3,5 27 3, 6 27 3, 7 27 3,8 27 3,9 27 3,10 27 3,11 27 3, 35 12 27 3,13 27 3,14 27 3,15 27 3,16 27 3,17 27 3,18 27 3,19 27 3, 20 27 3,21 27 3, 22 27 3, 23 27 3, 24 27 3, 25 27 3, 26 27 3, 27 27 3, 28 27 3,29 27 3,30 273,31 27 3, 32 27 3,33 27 3, 34 27 3, 35 27 3,36 27 3,37 27 3, 38 27 3,39 27 3,40 27 3,41 27 3, 16 Printed from Mimosa 13 07 15 42 27 3, 43 27 3, 44 27 3, 45 27 3, 46 27 3, 47 27 3,48 27 3,49 27 3,50 27 3, 51 27 3 , 52 27 3, 53 27 3, 54 27 3,1 28 3, 2 28 3, 3 28 3 4 28 3, 5 28 3, 6 28 3, 7 28 3, 8 28 3, 9 28 3, 10 28 3,11 28 3, 12 28 3,13 28 3,14 28 3,15 28 3,16 28 3,17 28 3,18 28 3,19 28 3, 20 28 3, 21 28 3, 22 28 3, 23 28 3, 24 28 3, 25 28 3,26 28 3,27 28 3,28 28 3, 29 28 3, 5 30 28 3,31 28 3, 32 28 3,33 28 3,34 28 3,35 28 3,36 28 3,37 28 3,38 28 3,39 28 3, 40 28 3,41 28 3, 42 28 3,43 28 3, 44 28 3,45 28 3,46 28 3,47 28 3, 48 28 3, 49 28 3, 50 28 3, 51 28 3 ,52 28 3,53 28 3, 54 28 3, 1 29 3,2 29 3,3 29 3, 4 29 3, 5 29 3, 6 29 3, 7 29 3, 8 29 3, 9 29 3,10 29 3,11 293,12 29 3,13 29 3,14 29 3,15 29 3,16 29 3,17 29 3, 18 29 3,19 29 3, 20 29 3, 21 29 3( 22 29 3, 23 29 3,24 29 3,25 29 3, 26 29 3, 27 29 3, 10 28 29 3,29 29 3,30 29 3,31 29 3,32 293,33 29 3,34 29 3,35 29 3,36 29 3,37 29 3, 38 29 3,39 29 3, 40 29 3,41 29 3, 42 29 3, 43 29 3,44 29 3,45 29 3, 46 29 3, 47 29 3, 48 29 3, 49 29 3, 50 29 3,51 29 3 ,52 29 3,53 29 3,54 29 3,1 30 3, 2 30 3,3 30 3,4 30 3, 5 30 3, 6 30 3,7 30 3, 8 30 3,9 30 3,10 30 3,11 30 3,12 30 3, 13 30 3,14 30 3, 15 30 3, 16 30 3,17 30 3, 18 30 3,19 30 3, 20 30 3, 21 30 3. 22 30 3,23 30 3, 24 30 3, 25 30 3, 15 26 30 3, 27 30 3, 28 30 3, 29 30 3, 30 30 3, 31 30 3,32 30 3,33 30 3, 34 30 3,35 30 3, 36 30 3,37 30 3, 38 30 3,39 30 3,40 30 3,41 30 3,42 30 3,43 30 3.44 30 3, 45 30 3, 46 30 3,47 30 3, 48 30 3,49 30 3, 50 30 3, 51 30 3 ,52 30 3,53 30 3, 54 30 3,1 31 3, 231 3, 3 31 3, 4 313,5 31 3,6 313, 7 31 3,8 31 3,9 31 3,10 313,11 31 3,12 313,13 313, 14 31 3,15 313,16 31 3,17 31 3,18 31 3,19 31 3,20 313,21 31 3,22 313,23 313, 20 24 31 3, 25 31 3, 26 31 3,27 31 3,28 31 3,29 31 3,30 31 3,31 31 3,32 31 3,33 313, 34 31 3,35 31 3, 36 31 3,37 31 3,38 31 3,39 31 3,40 31 3,41 31 3, 42 31 3,43 31 3, 44 31 3,45 31 3, 46 31 3,47 31 3,48 31 3,49 31 3, 50 31 3,51 31 3 ,52 31 3, 53 31 3, 54 31 3,1 32 3,2 32 3, 3 32 3,4 32 3, 5 32 3, 6 32 3, 7 32 3,8 32 3,9 32 3,10 32 3,11 32 3, 12 32 3,13 32 3,14 32 3,15 32 3, 16 32 3,17 32 3,18 32 3,19 32 3, 20 32 3,21 32 3, 25 22 32 3, 23 32 3, 24 32 3, 25 32 3,26 32 3, 27 32 3, 28 32 3,29 32 3,30 32 3, 31 32 3, 32 32 3,33 32 3,34 32 3,35 32 3,36 32 3, 37 32 3,38 32 3,39 32 3, 40 32 3, 41 32 3, 42 32 3, 43 32 3, 44 32 3,45 32 3,46 32 3,47 32 3, 48 32 3,49 32 3,50 32 3, 51 32 3 , 52 32 3, 53 32 3, 54 32 3,1 33 3,2 33 3,3 33 3,4 33 3,5 33 3, 6 33 3, 7 33 3, 8 33 3, 9 33 3, 10 33 3,11 33 3,12 33 3,13 33 3,14 33 3,15 33 3,16 33 3,17 33 3,18 33 3,19 33 3, 30 20 33 3,21 33 3, 22 33 3,23 33 3,24 33 3,25 33 3,26 33 3,27 33 3,28 33 3,29 33 3, 30 33 3, 31 33 3,32 33 3, 33 33 3,34 33 3,35 33 3,36 33 3,37 33 3,38 33 3, 39 33 3, 40 33 3,41 33 3, 42 33 3,43 33 3, 44 33 3,45 33 3,46 33 3,47 33 3,48 33 3,49 33 3, 50 33 3, 51 33 3 , 52 33 3, 53 33 3,54 33 3,1 34 3, 2 34 3, 3 34 3, 4 34 3, 5 34 3,6 34 3, 7 34 3, 8 34 3, 9 34 3,10 34 3,11 34 3,12 34 3,13 34 3,14 34 3,15 343,16 34 3,17 34 3, 35 18 34 3,19 34 3, 20 34 3, 21 34 3, 22 34 3, 23 34 3,24 34 3,25 34 3, 26 34 3, 27 34 3, 28 34 3,29 34 3, 30 34 3, 31 34 3, 32 34 3,33 34 3,34 34 3,35 34 3, 36 34 3,37 34 3, 38 34 3,39 34 3, 40 34 3, 41 34 3, 42 34 3,43 34 3,44 34 3,45 34 3, 46 34 3, 47 34 3, 17 Printed from Mimosa 13 07 15 48 34 3,49 34 3, 50 34 3, 51 34 3 ,52 34 3, 53 34 3, 54 34 3,1 35 3,2 35 3, 3 35 3,4 35 3, 5 35 3, 6 35 3, 7 35 3, 8 35 3,9 35 3, 10 35 3,11 35 3,12 35 3,13 35 3,14 35 3,15 35 3, 16 35 3,17 35 3,18 35 3,19 35 3, 20 35 3, 21 35 3, 22 35 3, 23 35 3, 24 35 3, 25 35 3, 26 35 3,27 35 3, 28 35 3, 29 35 3, 30 35 3,31 35 3,32 35 3, 33 35 3,34 35 3,35 35 3, 5 36 35 3,37 35 3,38 35 3,39 35 3, 40 35 3, 41 35 3,42 35 3,43 35 3,44 35 3,45 35 3, 46 35 3,47 35 3,48 35 3, 49 35 3, 50 35 3, 51 35 3 ,52 35 3, 53 35 3, 54 35 3, 1 36 3,2 36 3, 3 36 3, 4 36 3,5 36 3, 6 36 3,7 36 3, 8 36 3,9 36 3,10 36 3,11 36 3,12 36 3,13 36 3, 14 36 3,15 36 3,16 36 3,17 3 6 3,18 36 3,19 36 3,20 3 6 3,21 36 3, 22 36 3,23 3 6 3, 24 36 3,25 36 3, 26 36 3,27 36 3, 28 36 3.29 36 3,30 36 3,31 36 3,32 36 3,33 36 3, 10 34 36 3,35 36 3,36 36 3,37 36 3,38 36 3,39 36 3,40 36 3,41 36 3,42 36 3,43 36 3, 44 36 3,45 36 3,46 36 3,47 36 3, 48 36 3,49 36 3, 50 36 3, 51 36 3 , 52 36 3, 53 36 3, 54 36 3,1 37 3, 2 37 3,3 37 3, 4 37 3,5 37 3( 6 373, 7 37 3,8 373,9 37 3, 10 37 3,11 37 3, 12 37 3,13 37 3,14 37 3,15 37 3,16 37 3,17 37 3,18 37 3,19 37 3, 20 37 3, 21 37 3, 22 37 3, 23 37 3, 24 37 3,25 37 3, 26 37 3, 27 37 3, 28 37 3, 29 37 3,30 37 3, 31 37 3, 15 32 37 3,33 37 3,34 37 3,35 37 3,36 37 3,37 37 3,38 37 3,39 37 3,40 37 3,41 37 3, 42 37 3,43 37 3,44 37 3,45 37 3, 46 37 3,47 37 3,48 37 3,49 37 3,50 37 3, 51 37 3 , 52 37 3, 53 373,54 373,1 38 3,2 38 3, 3 38 3,4 38 3,5 38 3, 6 38 3,7 38 3,8 38 3, 9 38 3, 10 38 3,11 38 3,12 38 3,13 38 3,14 38 3,15 38 3,16 38 3,17 38 3,18 38 3,19 38 3, 20 38 3, 21 38 3,22 38 3,23 38 3,24 38 3,25 38 3,26 38 3,27 38 3,28 38 3,29 38 3, 20 30 38 3, 31 38 3,32 38 3,33 38 3, 34 38 3,35 38 3,36 38 3,37 38 3, 38 38 3,39 38 3, 40 38 3,41 38 3,42 38 3, 43 38 3,44 38 3,45 38 3,46 38 3,47 38 3, 48 38 3,49 38 3, 50 38 3,51 383 ,52 38 3,53 38 3,54 38 3,139 3,2 39 3,3 39 3,4 39 3,5 39 3, 6 39 3, 7 39 3, 8 39 3,9 39 3,10 39 3, 11 39 3,12 39 3,13 39 3,14 39 3,15 39 3,16 39 3,17 39 3, 18 39 3,19 39 3, 20 39 3, 21 39 3,22 39 3,23 39 3,24 39 3,25 39 3, 26 39 3, 27 39 3, 25 28 39 3, 29 39 3,30 39 3, 31 39 3,32 39 3,33 39 3,34 39 3,35 39 3, % 39 3, 37 39 3, 38 39 3, 39 39 3,40 39 3,41 39 3,42 39 3,43 39 3,44 39 3, 45 39 3, 46 39 3, 47 39 3, 48 39 3, 49 39 3,50 39 3, 51 39 3 ,52 39 3,53 39 3, 5439 3,1 40 3, 2 40 3,3 40 3,4 40 3, 5 40 3, 6 40 3, 7 40 3, 8 40 3, 9 40 3,10 40 3,11 40 3,12 40 3,13 40 3,14 40 3,15 40 3, 16 40 3,17 40 3,18 40 3,19 40 3,20 40 3,21 40 3,22 40 3, 23 40 3, 24 40 3, 25 40 3, 30 26 40 3, 27 40 3,28 40 3, 29 40 3,30 40 3,31 40 3,32 40 3,33 40 3, 34 40 3,35 40 3, 36 40 3,37 40 3,38 40 3,39 40 3,40 40 3,41 40 3,42 40 3,43 40 3, 44 40 3, 45 40 3, 46 40 3,47 40 3,48 40 3, 49 40 3,50 40 3,5] 40 3 , 52 40 3,53 40 3,54 40 3,1 41 3, 2 41 3, 3 41 3,4 41 3, 5 41 3, 6 41 3, 7 41 3, 8 41 3, 9 41 3,10 41 3,11 41 3,12 41 3, 13 41 3, 14 41 3,15 41 3,16 413,17 41 3,18 41 3,19 41 3,20 41 3,21 41 3, 22 41 3, 23 41 3, 35 24 41 3, 25 41 3,26 41 3, 27 41 3,28 41 3,29 413,30 41 3,31 41 3, 32 41 3, 33 41 3, 34 41 3,35 41 3,36 41 3, 37 413,38 41 3,39 41 3,40 41 3,41 41 3, 42 41 3, 43 41 3, 44 41 3, 45 41 3, 46 41 3 47 41 3,48 41 3, 49 41 3,50 41 3,51 41 3 ,52 41 3,53 41 3, 18 Printed from Mimosa 13 07 15 54 41 3,1 42 3, 2 42 3,3 42 3,4 42 3, 5 42 3, 6 42 3,7 42 3, 8 42 3,9 42 3,10 42 3,11 42 3, 12 42 3,13 42 3,14 42 3,15 42 3,16 42 3,17 423, 18 42 3, 19 42 3, 20 42 3, 21 42 3, 22 42 3, 23 42 3, 24 42 3, 25 42 3, 26 42 3,27 42 3, 28 42 3, 29 42 3, 30 42 3,31 42 3, 32 42 3,33 42 3,34 42 3, 35 42 3, 36 42 3,37 42 3, 38 42 3,39 42 3,40 42 3,41 42 3, 42 42 3,43 42 3,44 42 3,45 42 3,46 42 3,47 42 3, 48 42 3,49 42 3,50 42 3, 51 42 3 , 52 42 3, 53 42 3, 54 42 3,1 43 3, 2 43 3, 3 43 3,4 43 3, 5 43 3, 6 43 3,7 43 3,8 43 3, 9 43 3, 43 3,11 43 3,12 43 3,13 43 3,14 43 3,15 433,16 43 3,17 433,18 43 3,19 43 3, 20 43 3,21 43 3,22 43 3, 23 43 3, 24 43 3, 25 433, 26 43 3, 27 43 3,28 43 3, 29 43 3, 43 3,31 43 3,32 43 3, 33 43 3,34 43 3,35 433, 36 43 3, 37 43 3,38 43 3,39 43 3, 10 40 43 3,41 43 3,42 43 3,43 43 3, 44 43 3,45 433, 46 43 3, 47 43 3,48 43 3,49 433, 50 43 3,51 43 3 , 52 43 3,53 43 3,54 43 3,1 44 3,2 44 3,3 44 3,4 44 3,5 44 3, 6 44 3, 744 3, 8 44 3,9 44 3,10 44 3,11 44 3,12 44 3,13 44 3,14 44 3,15 44 3,16 44 3,17 44 3, 18 44 3,19 44 3,20 44 3, 21 44 3, 22 44 3,23 44 3, 24 44 3,25 44 3, 26 44 3,27 44 3, 28 44 3,29 44 3,30 44 3,31 44 3, 32 44 3,33 44 3, 34 44 3,35 44 3,36 44 3,37 44 3, 38 44 3,39 44 3, 40 44 3, 41 44 3, 42 44 3,43 44 3, 44 44 3,45 44 3, 46 44 3,47 44 3, 48 44 3, 49 44 3,50 443, 51 44 3 , 52 44 3,53 44 3, 54 44 3,1 1 4, 2 1 4,3 1 4, 4 1 4, 5 1 4, 6 1 4, 7 1 4,8 1 4, 9 1 4,10 1 4,11 1 4,12 1 4,13 1 4,14 1 4,15 1 4,16 1 4,17 14,18 1 4, 19 1 4, 20 1 4,21 1 4, 22 1 4,23 1 4, 24 1 4, 25 1 4, 26 1 4,27 1 4, 28 1 4, 29 1 4,30 1 4, 31 1 4,32 1 4,33 1 4, 34 1 4,35 1 4, 36 1 4, 37 1 4, 38 1 4,39 1 4,40 1 4, 41 1 4,42 1 4, 20 43 1 4, 44 1 4,45 1 4, 46 1 4,47 1 4,48 1 4, 49 1 4, 50 1 4,51 1 3 ,52 1 4,53 1 4,54 1 4, 1 2 4,2 2 4,3 2 4, 4 2 4,5 2 4, 6 2 4, 7 2 4,8 2 4,9 2 4,10 2 4,11 2 4,12 2 4,13 2 4,14 2 4, 15 2 4,16 2 4,17 2 4,18 2 4,19 2 4,20 2 4, 2i 2.4, 22 2 4,23 2 4,24 2 4, 25 2 4,26 2 4, 27 2 4, 28 2 4,29 2 4, 30 2 4,31 2 4,32 2 4, 33 2 4, 34 2 4,35 2 4, 36 2 4,37 2 4,38 2 4, 39 2 4,40 2 4,412 4,42 2 4,43 2 4,44 2 4,45 2 4,46 2 4,472 4,48 24,49 2 4,50 2 4, 25 51 2 3 , 52 2 4, 53 2 4, 54 2 4,1 3 4, 2 3 4,3 3 4,4 3 4, 5 3 4, 6 3 4, 7 3 4,8 3 4, 9 3 4,10 3 4, 11 3 4,12 3 4,13 3 4,14 3 4, 15 3 4,16 3 4, 17 3 4, 18 3 4,19 3 4, 20 3 4, 21 3 4,22 3 4, 23 3 4,24 3 4,25 3 4, 26 3 4, 27 3 4, 28 3 4, 29 3 4, 30 3 4,31 3 4, 32 3 4, 33 3 4,34 3 4, 35 3 4,36 3 4,37 3 4,38 3 4,39 3 4,40 3 4, 41 3 4, 42 3 4,43 3 4,44 3 4, 45 3 4,46 3 4, 47 3 4,48 3 4,49 3 4,50 3 4,51 3 3 , 52 3 4,53 3 4, 54 3 4,1 4 4,2 4 4,3 4 4,4 4 4,5 4 4, 6 4 4, 7 4 4,8 4 4, 9 4 4,10 4 4,11 4 4,12 4 4,13 4 4,14 4 4,15 4 4,16 4 4,17 4 4,18 4 4, 19 4 4,20 4 4,21 4 4, 22 4 4, 23 4 4,24 4 4,25 4 4, 26 4 4,27 4 4,28 4 4,29 4 4,30 4 4, 31 4 4,32 4 4,33 4 4,34 4 4,35 4 4,36 4 4,37 4 4, 38 4 4,39 4 4,40 4 4,41 4 4,42 4 4, 43 4 4,44 4 4,45 4 4,46 4 4, 47 4 4,48 4 4, 49 4 4, 50 4 4,51 4 3 , 52 4 4, 53 4 4, 54 4 4, 1 5 4, 2 5 4, 3 5 4, 4 5 4, 5 5 4, 6 5 4, 7 5 4,8 5 4, 9 5 4, 10 5 4, 11 5 4,12 5 4,13 5 4,14 5 4, 35 15 5 4,16 5 4,17 5 4,18 5 4,19 5 4,20 5 4, 21 5 4, 22 5 4,23 5 4,24 5 4,25 5 4,26 5 4, 27 5 4,28 5 4,29 5 4,30 5 4,31 5 4,32 5 4, 33 5 4, 34 5 4,35 5 4,36 5 4,37 5 4,38 5 4, 39 5 4,40 5 4,41 5 4, 42 5 4, 43 5 4,44 5 4,45 5 4, 46 5 4,47 5 4, 48 5 4,49 5 4,50 5 4, 19 Printed from Mimosa 13 07 15 PC/YUS96/20226 51 5 3 , 52 5 4, 53 5 4, 54 5 4, 1 6 4, 2 6 4,3 6 4, 4 6 4, 5 6 4, 6 6 4, 7 6 4, 8 6 4, 9 6 4,10 6 4, 11 6 4,12 6 4,13 6 4, 14 6 4,15 6 4, 16 6 4,17 6 4,18 6 4, 19 6 4, 20 6 4, 21 6 4,22 6 4, 23 6 4, 24 6 4,25 6 A, 26 6 4, 27 6 4, 28 6 4, 29 6 4, 30 6 4,31 6 4, 32 6 4,33 6 4,34 6 4, 35 6 4, 36 6 4,37 6 4, 38 6 4,39 6 4, 40 6 4,41 6 4, 42 6 4,43 6 4, 44 6 4,45 6 4,46 6 4, 5 47 6 4, 48 6 4,49 6 4, 50 6 4,51 6 3 ,52 6 4,53 6 4, 54 6 4,1 7 4, 2 7 4,3 7 4,4 7 4, 5 7 4, 6 74,774,874,974,1074,1174,1274,1374,1474,1574, 1674,1774,1874, 19 7 4, 20 7 4,21 7 4,22 7 4, 23 7 4, 24 7 4,25 7 4,26 7 4, 27 7 4, 28 7 4,29 7 4,30 7 4, 31 7 4,32 7 4,33 7 4, 34 7 4,35 7 4,36 7 4,37 7 4,38 7 4,39 7 4, 40 7 4,41 7 4,42 7 4, 43 7 4, 44 7 4,45 7 4,46 7 4,47 7 4, 48 7 4,49 7 4,50 7 4,51 7 3 , 52 7 4, 53 7 4, 54 7 4, 10 1 8 4,2 8 4,3 8 4, 4 8 4, 5 8 4,6 8 4, 7 8 4, 8 8 4,9 8 4,10 8 4,11 8 4,12 8 4,13 8 4,14 8 4, 8 4,16 8 4,17 8 4,18 8 4,19 8 4, 20 8 4,21 8 4, 22 8 4, 23 8 4, 24 8 4, 25 8 4, 26 8 4, 27 8 4, 28 8 4,29 8 4,30 8 4,31 8 4, 32 8 4,33 8 4,34 8 4,35 8 4, 36 8 4,37 8 4,38 8 4, 39 8 4,40 8 4,41 8 4,42 8 4,43 8 4, 44 8 4,45 8 4,46 8 4, 47 8 4,48 8 4, 49 8 4, 50 8 4, 51 8 3 ,52 8 4, 53 8 4, 54 8 4,1 9 4, 2 9 4, 3 9 4, 4 9 4, 5 9 4,6 9 4,7 9 4, 8 9 4,9 9 4,10 9 4, 15 1194,1294,1394,1494,1594,1694 179 4,1894,1994,2094,2194,2294, 23 9 4,24 9 4, 25 9 4, 26 9 4, 27 9 4, 28 9 4,29 9 4, 30 9 4, 31 9 4,32 9 4,33 9 4,34 9 4, 9 4,36 9 4,37 9 4,38 9 4,39 9 4,40 9 4,41 9 4, 42 9 4, 43 9 4,44 9 4,45 9 4,46 9 4, 47 9 4,48 9 4,49 9 4,50 9 4, 51 93 , 52 9 4, 53 9 4, 54 9 4,110 4,2 10 4, 3 10 4, 4 10 4, 5 10 4, 6 10 4, 710 4, 8 10 4, 9 10 4,10 10 4,11 10 4,12 10 4,13 10 4,14 10 4,15 10 4, 16 10 4,1710 4,18 10 4,19 10 4,20 10 4, 2110 4, 22 10 4, 23 10 4,24 10 4, 25 10 4, 26 10 4,2710 4,28 10 4, 29 10 4,30 10 4,31 10 4, 32 10 4,33 10 4,34 10 4,35 10 4, 36 10 4,3710 4,38 10 4, 39 10 4,40 10 4,41 10 4, 42 10 4, 43 10 4,44 10 4,45 10 4, 46 10 4,47 10 4,48 10 4, 49 10 4,50 10 4,51 10 3 ,5210 4,53 10 4,54 10 4,111 4, 2 11 4, 3 11 4,4 11 4, 5 11 4, 6 11 4, 7 11 4, 8 11 4,9 11 4,10 11 4,11 11 4,12 11 4,13 11 4, 25 14 11 4,15 11 4,16 11 4,17 11 4,18 11 4,19 11 4, 20 11 4, 21 11 4, 22 11 4,23 11 4, 24 11 4, 25 11 4,26 11 4, 27 11 4,28 11 4, 29 11 4, 30 11 4,31 11 4,32 11 4,33 11 4, 34 11 4, 35 11 4,36 11 4,37 11 4,38 11 4,39 11 4,40 11 4,41 11 4,42 11 4,43 11 4, 44 11 4, 45 11 4,46 11 4, 47 11 4,48 11 4,49 11 4, 50 11 4, 5111 3 ,5211 4,53 11 4, 54 11 4,112 4, 2 12 4,3 12 4, 4 12 4, 5 12 4, 6 12 4,7 12 4,8 12 4, 9 12 4,10 12 4,11 12 4, 30 12 12 4,13 12 4,14 12 4,15 12 4,16 12 4,1712 4,1812 4,19 12 4,20 12 4,21 12 4, 22 12 4,23 12 4, 24 12 4,25 12 4,26 12 4,27 12 4,28 12 4,29 12 4,30 12 4,31 12 4, 32 12 4,33 12 4, 34 12 4,35 12 4,36 12 4,37 12 4,38 12 4,39 12 4,40 12 4,41 12 4, 42 12 4, 43 12 4, 44 12 4,45 12 4,46 12 4,47 12 4,48 12 4,49 12 4,50 12 4,51 12 3 , 52 12 4, 53 12 4, 54 12 4,1 13 4,2 13 4,3 13 4,4 13 4,5 13 4, 6 13 4, 7 13 4,8 13 4, 9 13 4, 35 10 13 4,11 13 4,12 13 4,13 13 4,14 13 4,15 13 4,16 13 4,17 13 4,18 13 4,19 13 4, 13 4, 21 13 4, 22 13 4,23 13 4, 24 13 4,25 13 4, 26 13 4,27 13 4, 28 13 4, 29 13 4, 30 13 4,31 13 4,32 13 4,33 13 4,34 13 4,35 13 4,36 13 4,3713 4,38 13 4,39 13 4, Printed from Mimosa 13 07 15 PCIYUS96/20226 40 13 4,41 13 4, 42 13 4,43 13 4, 44 13 4,45 13 4,46 13 4,47 13 4,48 13 4,49 13 4, 50 13 4, 51 13 3 , 52 13 4,53 13 4,54 13 4,1 14 4,2 14 4, 3 14 4,4 14 4,5 14 4, 6 14 4, 7 14 4,8 14 4, 9 14 4, 10 14 4,11 14 4,12 14 4,13 14 4, 14 14 4,15 14 4,16 14 4,17 14 4, 18 14 4,19 14 4, 20 14 4, 21 14 4, 22 14 4,23 14 4,24 14 4,25 14 4,26 14 4, 27 14 4, 5 28 14 4, 29 14 4,30 14 4,31 14 4,32 14 4,33 14 4,34 14 4,35 14 4,36 14 4,37 14 4, 38 14 4, 39 14 4,40 14 4,41 14 4,42 14 4,43 14 4,44 14 4,45 14 4,46 14 4, 47 14 4, 48 14 4, 49 14 4, 50 14 4,51 14 3 ,52 14 4,53 14 4,54 14 4,1 15 4,2 15 4,3 15 4,4 15 4, 5 15 4, 6 15 4( 7 15 4, 8 15 4,9 15 4,10 15 4,11 15 4,12 15 4,13 15 4,14 15 4,15 15 4, 16 15 4,1715 4,18 15 4,19 15 4,20 15 4,2115 4,22 15 4,23 15 4,24 15 4,25 15 4, 10 26 15 4, 27 15 4, 28 15 4, 29 15 4,30 15 4,3115 4,32 15 4,33 15 4,34 15 4,35 15 4, 36 15 4,37 15 4,38 15 4,39 15 4,40 15 4,4115 4,42 15 4,43 15 4,44 15 4,45 15 4, 46 15 4,4715 4, 48 15 4,49 15 4, 50 15 4,51 15 3 , 52 15 4,53 15 4, 54 15 4,1 16 4 2 16 4, 3 16 4,4 16 4,5 16 4, 6 16 4, 7 16 4,8 16 4, 9 16 4,10 16 4,11 16 4 12 16 4,13 16 4, 14 16 4,15 16 4,16 16 4,17 16 4,18 16 4,19 16 4, 20 16 4,21 16 4,22 16 4, 23 16 4, 15 24 16 4, 25 16 4, 26 16 4, 27 16 4, 28 16 4,29 16 4,30 16 4,31 16 4,32 16 4,33 16 4, 34 16 4, 35 16 4, 36 16 4. 37 16 4,38 16 4,39 16 4,40 16 4,41 16 4,42 16 4,43 16 4, 44 16 4, 45 16 4, 46 16 4, 47 16 4,48 16 4,49 16 4,50 16 4, 5116 3 , 52 16 4, 53 16 4, 54 16 4,117 4,2 174,3 17 4, 4 17 4,5 17 4, 6 17 4, 7 17 4,8 17 4, 9 17 4,10 17 4,11 17 4, 12 17 4, 13 17 4,14 17 4,15 17 4,16 17 4,17 17 4,18 17 4,19 17 4,20 17 4, 21 17 4, 20 22 17 4, 23 17 4, 24 17 4, 25 17 4, 26 17 4,27 17 4,28 17 4,29 17 4,30 17 4, 31 17 4, 32 17 4, 33 17 4, 34 17 4,35 17 4,36 17 4,37 17 4, 38 17 4,39 17 4,40 17 4,41 17 4, 42 17 4, 43 17 4,44 17 4,45 17 4,46 17 4,47 17 4,48 17 4,49 17 4,50 17 4, 51 17 3, 52 17 4, 53 17 4, 54 17 4,1 18 4,2 18 4,3 18 4,4 18 4,5 18 4, 6 18 4, 7 18 4,8 18 4,9 18 4, 10 18 4,11 18 4,12 18 4,13 18 4,14 18 4,15 18 4,16 18 4,1718 4,18 18 4,19 18 4, 25 20 18 4, 21 18 4, 22 18 4, 23 18 4, 24 18 4,25 18 4,26 18 4, 27 18 4,28 18 4,29 18 4, 30 18 4, 31 18 4, 32 18 4, 33 18 4,34 18 4,35 18 4,36 18 4,37 18 4,38 18 4,39 18 4, 40 18 4,41 18 4,42 18 4,43 18 4,44 18 4,45 18 4,46 18 4,47 18 4,48 18 4,49 18 4, 50 18 4,51 18 3 , 52 18 4,53 18 4,54 18 4,1 19 4, 2 19 4, 3 19 4,4 19 4, 5 19 4, 6 19 4, 7 19 4,8 19 4,9 19 4,10 19 4,11 19 4,12 19 4,13 19 4,14 19 4,15 19 4,16 19 4,17 19 4, 30 18 19 4,19 19 4, 20 19 4, 21 19 4, 22 19 4,23 19 4, 24 19 4,25 19 4,26 19 4, 27 19 4, 28 19 4, 29 19 4, 30 19 4,31 19 4, 32 19 4,33 19 4,34 19 4,35 19 4,36 19 4,37 19 4, 38 19 4, 39 19 4,40 19 4,41 19 4,42 19 4,43 19 4,44 19 4,45 19 4,46 19 4,47 19 4, 48 19 4, 49 19 4, 50 19 4, 51 19 3 ,52 19 4,53 19 4, 54 19 4,1 20 4, 2 20 4, 3 20 4,4 20 4, 5 20 4,6 20 4, 7 20 4,8 20 4,9 20 4,10 20 4,11 20 4,12 20 4,13 20 4,14 20 4,15 20 4, 35 16 20 4,17 20 4,18 20 4,19 20 4, 20 20 4,21 20 4, 22 20 4, 23 20 4,24 20 4, 25 20 4, 26 20 4, 27 20 4,28 20 4,29 20 4,30 20 4,31 20 4,32 20 4,33 20 4,34 20 4,35 20 4, 36 20 4, 37 20 4,38 20 4, 39 20 4, 40 20 4,41 20 4,42 20 4,43 20 4,44 20 4, 45 20 4, 21 Printed from Mimosa 13 07 15 PCIYUS96/20226 46 20 4, 47 20 4, 48 20 4, 49 20 4, 50 20 4, 51 20 3 ,52 20 4,53 20 4, 54 20 4, 1 21 4, 2 21 4, 3 21 4, 4 21 4, 5 21 4, 6 21 4,7 21 4, 8 21 4,9 21 4,10 21 4,11 21 4,12 21 4,13 21 4, 14 21 4, 15 21 4,16 21 4, 17 21 4, 18 21 4,19 21 4,20 21 4, 21 21 4,22 21 4,23 21 4, 24 21 4, 25 21 4,26 21 4, 27 21 4, 28 21 4,29 21 4,30 21 4,31 21 4,32 21 4, 33 21 4, 5 34 21 4,35 21 4,36 21 4, 37 21 4, 38 21 4,39 21 4,40 21 4, 41 21 4,42 21 4,43 21 4, 44 21 4, 45 21 4, 46 21 4, 47 21 4, 48 21 4,49 21 4,50 21 4,51 21 3 ,52 21 4, 53 21 4, 54 21 4,1 22 4, 2 22 4,3 22 4,4 22 4,5 22 4, 6 22 4, 7 22 4,8 22 4, 9 22 4,10 22 4,11 224, 12 22 4,13 22 4,14 22 4,15 22 4,16 22 4,17 22 4,18 22 4,19 22 4,20 22 4,21 22 4, 22 22 4, 23 22 4, 24 22 4, 25 22 4, 26 22 4,27 22 4,28 22 4,29 224,30 22 4,31 22 4, 10 32 22 4, 33 22 4,34 22 4,35 22 4,36 22 4,37 22 4,38 22 4,39 22 4,40 22 4,41 22 4, 42 22 4,43 22 4, 44 22 4, 45 22 4, 46 22 4,47 22 4,48 22 4,49 22 4,50 22 4, 51 22 3 , 52 22 4, 53 22 4, 54 22 4,1 23 4,2 23 4,3 23 4,4 23 4,5 23 4,6 23 4, 7 23 4,8 23 4, 9 23 4, 10 23 4,11 23 4,12 23 4,13 23 4, 14 23 4,15 23 4,16 23 4,17 23 4,18 23 4,19 23 4, 20 23 4, 21 23 4, 22 23 4, 23 23 4, 24 23 4,25 23 4,26 23 4, 27 23 4, 28 23 4, 29 23 4, 15 30 23 4,31 23 4,32 23 4, 33 23 4,34 23 4,35 23 4,36 23 4,37 23 4,38 23 4,39 23 4, 40 23 4,41 23 4,42 23 4, 43 23 4,44 23 4,45 23 4,46 23 4,47 23 4,48 23 4,49 23 4, 50 23 4, 51 23 3 ,52 23 4,53 23 4,54 23 4,1 24 4, 2 24 4,3 24 4,4 24 4, 5 24 4, 6 24 4, 7 24 4, 8 24 4,9 24 4, 10 24 4,11 24 4,12 24 4,13 24 4,14 24 4,15 24 4,16 24 4,17 24 4, 18 24 4,19 24 4, 20 24 4, 21 24 4, 22 24 4,23 24 4,24 24 4,25 24 4,26 24 4,27 24 4, 20 28 24 4,29 24 4,30 24 4, 31 24 4,32 24 4,33 24 4,34 24 4,35 24 4,36 24 4,37 24 4, 38 24 4, 39 24 4,40 24 4, 41 24 4,42 24 4,43 24 4,44 24 4,45 24 4,46 24 4,47 24 4, 48 24 4, 49 24 4,50 24 4, 51 24 3 , 52 24 4, 53 24 4,54 24 4,1 25 4,2 25 4,3 25 4,4 25 4, 5 25 4, 6 25 4,7 25 4, 8 25 4,9 25 4,10 25 4,11 25 4,12 25 4,13 25 4,14 25 4,15 25 4, 16 25 4,17 25 4,18 25 4,19 25 4, 20 25 4, 21 25 4,22 25 4,23 25 4,24 25 4, 25 25 4, 25 26 25 4, 27 25 4,28 25 4, 29 25 4,30 25 4,31 25 4,32 25 4,33 25 4,34 25 4,35 25 4, 36 25 4,37 25 4,38 25 4, 39 25 4,40 25 4,41 25 4,42 25 4,43 25 4,44 25 4,45 25 4, 46 25 4, 47 25 4,48 25 4, 49 25 4,50 25 4, 51 253 ,52 25 4,53 25 4,54 25 4,1 26 4, 2 26 4, 3 26 4,4 26 4,5 26 4, 6 26 4,7 26 4, 8 26 4,9 26 4,10 26 4,11 26 4,12 26 4,13 26 4, 14 26 4,15 26 4,16 26 4, 17 26 4,18 26 4,19 26 4,20 26 4,21 26 4,22 26 4, 23 26 4, 30 24 26 4,25 26 4,26 26 4, 27 26 4,28 26 4,29 26 4,30 26 4,31 26 4,32 26 4,33 26 4, 34 26 4,35 26 4,36 26 4, 37 26 4,38 26 4,39 26 4,40 26 4,41 26 4,42 26 4,43 26 4, 44 26 4, 45 26 4,46 26 4, 47 26 4,48 26 4,49 26 4,50 26 4,51 26 3 ,52 26 4, 53 26 4, 54 26 4,1 27 4, 2 27 4,3 27 4, 4 27 4,5 27 4, 6 27 4, 7 274,8 27 4, 9 27 4,10 27 4,11 27 4, 12 27 4,13 27 4,14 27 4,15 27 4,16 27 4,17 27 4,18 27 4,19 27 4,20 27 4, 21 27 4, 35 22 27 4,23 27 4,24 27 4, 25 27 4, 26 27 4,27 27 4,28 27 4,29 27 4,30 27 4,31 27 4, 32 27 4,33 27 4,34 27 4,35 27 4, 36 27 4,37 27 4,38 27 4,39 27 4,40 27 4,41 27 4, 42 27 4, 43 27 4,44 27 4, 45 27 4,46 27 4,47 27 4,48 27 4, 49 27 4,50 27 4, 51 27 3 , 22 Printed from Mimosa 13 07 15 52 27 4, 53 27 4,54 27 4,1 28 4, 2 28 4, 3 28 4, 4 28 4, 5 28 4, 6 28 4,7 28 4, 8 28 4, 9 28 4, 10 28 4,11 28 4,1? 28 4,13 28 4,14 28 4,15 28 4,16 28 4,17 28 4, 18 28 4,19 28 4, 20 28 4, 21 28 4,22 28 4, 23 28 4,24 28 4, 25 28 4, 26 28 4, 27 28 4, 28 28 4, 29 28 4, 30 28 4, 31 28 4,32 28 4,33 28 4,34 28 4,35 28 4, 36 28 4,37 28 4,38 28 4, 39 28 4, 5 40 28 4, 41 28 4,42 28 4,43 28 4,44 28 4,45 28 4,46 28 4,47 28 4,48 28 4,49 28 4, 50 28 4, 51 28 3 , 52 28 4,53 28 4, 54 28 4,1 29 4, 2 29 4, 3 29 4,4 29 4,5 29 4, 6 29 4, 7 29 4, 8 29 4, 9 29 4,10 29 4,11 29 4,12 29 4,13 29 4,14 29 4,15 29 4,16 29 4,17 29 4, 18 29 4,19 29 4,20 29 4,21 29 4,22 29 4, 23 29 4, 24 29 4, 25 29 4, 26 29 4, 27 29 4, 28 79 4, 29 29 4,30 29 4,31 29 4,32 29 4,33 29 4,34 29 4, 35 29 4, 36 29 4,37 29 4, 10 38 29 4, 39 29 4,40 29 4,41 29 4,42 29 4,43 29 4,44 29 4,45 29 4,46 29 4,47 29 4, 48 29 4,49 29 4,50 29 4,51 29 3 ,52 29 4,53 29 4,54 29 4,1 30 4,2 30 4,3 30 4,4 30 4, 5 30 4, 6 30 4,7 30 4, 8 30 4,9 30 4,10 30 4,11 30 4, 12 30 4,13 30 4,14 30 4,15 30 4, 16 30 4,17 30 4,18 30 4,19 30 4,20 30 4, 21 30 4, 22 30 4, 23 30 4, 24 30 4, 25 30 4, 26 30 4, 27 30 4,28 30 4, 29 30 4,30 30 4,31 30 4,32 30 1,33 30 4, 34 30 4,35 30 4, 15 36 3 0 4, 37 3 0 4,38 30 4, 39 3 0 4,40 30 4, 41 30 4, 42 30 4,43 30 4, 44 30 4,45 30 4, 46 30 4, 47 30 4,48 30 4,49 30 4,50 30 4,51 30 3 , 52 30 4, 53 30 4,54 30 4,1 31 4, 2 31 4, 3 31 4,4 31 4,5 31 4, 6 31 4,7 314,8 31 4, 9 31 4, 10 31 4,11 31 4,12 31 4,13 31 4, 14 31 4,15 31 4,16 31 4,17 31 4,18 31 4,19 31 4, 20 31 4, 21 31 4, 22 31 4, 23 31 4, 24 31 4, 25 31 4,26 31 4, 27 314,28 31 4,29 31 4,30 31 4, 31 31 4,32 31 4,33 31 4, 20 34 31 4, 35 31 4,36 31 4,37 314,38 31 4,39 31 4( 40 31 4, 41 31 4,42 31 4, 43 31 4, 44 31 4, 45 31 4,46 31 4,47 314,48 31 4,49 31 4, 50 31 4, 51 31 3 ,52 31 4, 53 31 4, 54 31 4,1 32 4, 2 32 4,3 32 4,4 32 4, 5 32 4, 6 32 4, 7 32 4, 8 32 4, 9 32 4, 10 32 4,11 32 4, 12 32 4,13 32 4,14 32 4,15 32 4,16 32 4,17 32 4,18 32 4, 19 32 4, 20 32 4, 21 32 4, 22 32 4, 23 32 4, 24 32 4,25 32 4,26 32 4, 27 32 4, 28 32 4, 29 32 4,30 32 4,31 32 4, 25 32 32 4, 33 32 4,34 32 4,35 32 4,36 32 4,37 32 4,38 32 4, 39 32 4, 40 32 4, 41 32 4, 42 32 4, 43 32 4,44 32 4,45 32 4,46 32 4,47 32 4, 48 32 4, 49 32 4, 50 32 4,51 32 3 , 52 32 4. 53 32 4,54 32 4,1 33 4, 2 33 4, 3 33 4,4 33 4,5 33 4, 6 33 4,7 33 4, 8 33 4, 9 33 4, 10 33 4,11 33 4,12 33 4,13 33 4,14 33 4,15 33 4,16 33 4,17 33 4,18 33 4,19 33 4, 20 33 4, 21 33 4,22 33 4,23 33 4,24 33 4,25 33 4, 26 33 4, 27 33 4, 28 33 4, 29 33 4, 30 30 33 4,31 33 4,32 33 4,33 33 4,34 33 4,35 33 4,36 33 4,37 33 4,38 33 4,39 33 4, 40 33 4, 41 33 4,42 33 4,43 33 4,44 33 4,45 33 4, 46 33 4, 47 33 4, 48 33 4,49 33 4, 50 33 4, 51 33 3, 52 33 4,53 33 4, 54 33 4,1 34 4,2 34 4, 3 34 4, 4 34 4,5 34 4, 6 34 4, 7 34 4, 8 34 4,9 34 4,10 34 4,11 34 4,12 34 4,13 34 4,14 34 4,15 34 4,16 34 4,17 34 4, 18 34 4,19 34 4, 20 34 4, 21 34 4,22 34 4, 23 34 4, 24 34 4, 25 34 4, 26 34 4, 27 34 4, 35 28 34 4, 29 34 4, 30 34 4,31 34 4,32 34 4,33 34 4, 34 34 4,35 34 4, 36 34 4, 37 34 4, 38 34 4, 39 34 4,40 34 4,41 34 4,42 34 4,43 34 4,44 34 4,45 34 4, 46 34 4,47 34 4, 48 34 4, 49 34 4, 50 34 4, 51 34 3 , 52 34 4,53 34 4,54 34 4,1 35 4, 2 35 4,3 35 4, 4 35 4, 23 Printed from Mimosa 13 07 15 35 4, 6 35 4, 7 35 4, 8 35 4,9 35 4, 10 35 4,11 35 4,12 35 4,13 35 4,14 35 4,15 35 4, 16 3 5 4, 17 35 4,18 35 4, 19 35 4, 20 35 4,21 35 4, 22 35 4,23 35 4, 24 35 4 25 35 4, 26 35 4, 27 35 4, 28 35 4, 29 35 4,30 35 4,31 35 4,32 35 4,33 35 4, 34 35 4, 35 35 4, 36 35 4, 37 35 4,38 35 4,39 35 4,40 35 4,41 35 4,42 35 4,43 35 4, 44 35 4, 45 35 4, 5 46 35 4,47 35 4,48 35 4,49 35 4.50 35 4,51 35 3 , 52 35 4,53 35 4,54 35 4,136 4,2 36 4, 3 36 4,4 36 4, 5 36 4, 6 36 4 7 36 4, 8 36 4, 9 36 4,10 36 4,11 36 4,12 36 4, 13 36 4, 14 36 4, 15 36 4,16 36 4,17 36 4,18 36 4,19 36 4, 20 36 4, 21 36 4, 22 36 4, 23 36 4, 24 36 4, 25 36 4,26 36 4,27 36 4, 28 36 4,29 36 4, 30 36 4,31 36 4, 32 36 4, 33 36 4, 34 36 4,35 36 4,36 36 4,37 36 4,38 36 4,39 36 4, 40 36 4,41 36 4, 42 36 4, 43 36 4, 10 44 36 4, 45 36 4,46 36 4,47 36 4,48 36 4,49 36 4, 50 36 4,51 36 3 . 52 36 4, 53 36 4, 54 36 4,1 37 4,2 37 4,3 37 4,4 37 4,5 37 4,6 37 4,7 37 4,8 37 4,9 37 4,10 37 4,11 37 4, 12 37 4,13 37 4,14 37 4,15 37 4,16 37 4,17 37 4,18 37 4,19 37 4, 20 37 4, 21 37 4, 22 37 4, 23 37 4,24 37 4,25 37 4, 26 37 4,27 37 4, 28 37 4, 29 37 4, 30 37 4, 31 37 4, 32 37 4, 33 37 4,34 37 4,35 37 4,36 37 4,37 37 4,38 37 4,39 37 4, 40 37 4, 41 37 4, 15 42 37 4, 43 37 4,44 37 4,45 37 4, 46 37 4,47 37 4,48 37 4,49 37 4, 50 37 4, 51 37 3 , 52 37 4, 53 37 4, 54 37 4,1 38 4,2 38 4,3 38 4,4 38 4,5 38 4, 6 38 4,7 38 4, 8 38 4,9 38 4, 10 38 4,11 38 4,12 38 4,13 38 4,14 38 4,15 38 4,16 38 4,17 38 4,18 38 4, 19 38 4, 20 38 4, 21 38 4,22 38 4,23 38 4,24 38 4,25 38 4, 26 38 4,27 38 4, 28 38 4, 29 38 4, 30 38 4, 31 38 4,32 38 4,33 38 4,34 38 4,35 38 4,36 38 4,37 38 4, 38 38 4, 39 38 4, 20 40 38 4,41 38 4,42 38 4, 43 38 4,44 38 4,45 38 4,46 38 4,47 38 4, 48 38 4, 49 38 4, 50 38 4, 51 38 3 , 52 38 4,53 38 4,54 38 4,1 39 4, 2 39 4,3 39 4,4 39 4, 5 39 4, 6 39 4, 7 39 4,8 39 4,9 39 4,10 39 4,11 39 4,12 39 4,13 39 4,14 39 4,15 39 4,16 39 4,17 39 4, 18 39 4,19 39 4, 20 39 4, 21 39 4,22 39 4,23 39 4,24 39 4,25 39 4, 26 39 4, 27 39 4, 28 39 4, 29 39 4,30 39 4,31 39 4,32 39 4,33 39 4,34 39 4, 35 39 4, 36 39 4, 37 39 4, 25 38 39 4, 39 39 4,40 39 4, 41 39 4,42 39 4,43 39 4,44 39 4, 45 39 4,46 39 4, 47 39 4, 48 39 4, 49 39 4, 50 39 4, 51 39 3 ,52 39 4, 53 39 4,54 39 4,1 40 4, 2 40 4,3 40 4, 4 40 4, 5 40 4, 6 40 4, 7 40 4, 8 40 4,9 40 4, 10 40 4,11 40 4,12 40 4,13 40 4,14 40 4,15 40 4, 16 40 4,17 40 4,18 40 4,19 40 4,20 40 4,21 40 4,22 40 4,23 40 4, 24 40 4, 25 40 4, 26 40 4, 27 40 4, 28 40 4, 29 40 4,30 40 4,31 40 4,32 40 4,33 40 4,34 40 4, 35 40 4, 30 36 40 4, 37 40 4,38 40 4,39 40 4,40 40 4,41 40 4,42 40 4,43 40 4,44 40 4, 45 40 4, 46 40 4,47 40 4,48 40 4,49 40 4,50 40 4,51 40 3 ,52 40 4, 53 40 4,54 40 4, 1 41 4,2 41 4, 3 41 4,4 41 4,5 41 4, 6 41 4,7 41 4, 8 41 4, 9 41 4,10 41 4,11 41 4,12 41 4, 13 41 4, 14 41 4,15 41 4,16 41 4,17 41 4,18 41 4,19 41 4, 20 41 4, 21 41 4, 22 41 4, 23 41 4, 24 41 4, 25 41 4, 26 41 4,27 41 4,28 41 4,29 41 4,30 41 4,31 41 4,32 41 4, 33 41 4, 35 34 41 4,35 41 4,36 41 4,37 41 4,38 41 4,39 41 4, 40 41 4, 41 41 4,42 41 4, 43 41 4, 44 41 4,45 41 4,46 41 4,47 414,48 41 4,49 41 4,50 41 4,51 41 3 ,52 41 4,53 41 4, 54 41 4,1 42 4, 2 42 4, 3 42 4, 4 42 4,5 42 4, 6 42 4, 7 42 4, 8 42 4,9 42 4,10 42 4, 11 42 4, 24 Printed from Mimosa 13 0715 12 42 4, 13 42 4, 14 42 4,15 42 4, 16 42 4, 17 42 4,18 42 4,19 42 4,20 42 4,21 42 4, 22 42 4, 23 42 4, 24 42 4, 25 42 4, 26 42 4, 27 42 4, 28 42 4,29 42 4,30 42 4,31 42 4, 32 42 4,33 42 4, 34 42 4,35 42 4, 36 42 4, 37 42 4, 38 42 4,39 42 4,40 42 4,41 42 4, 42 42 4,43 42 4, 44 42 4,45 42 4, 46 42 4,47 42 4, 48 42 4,49 42 4,50 42 4, 51 42 3 , 52 42 4,53 42 4, 54 42 4,1 43 4,2 43 4,3 43 4,4 43 4.5 43 4, 6 43 4, 7 43 4, 8 43 4, 9 43 4, 10 43 4,11 43 4, 12 43 4,13 43 4, 14 43 4,15 43 4, 16 43 4,17 43 4,18 43 4,19 43 4, 20 43 4,21 43 4, 22 43 4, 23 43 4, 24 43 4, 25 43 4, 26 43 4,27 43 4,2S 43 4,29 43 4, 43 4,31 43 4, 32 43 4, 33 43 4, 34 43 4, 35 43 4, 36 43 4,37 43 4,38 43 4,39 43 4, 40 43 4,41 43 4,42 43 4, 43 43 4, 44 43 4, 45 43 4, 46 43 4,47 43 4,48 43 4,49 43 4, 50 43 4,51 43 3 , 52 43 4,53 43 4,54 43 4,1 444,244 4,344 4,4 44 4,544 4,6444, 7 44 4,8 44 4,9 44 4,10 44 4,11 44 4,12 44 4,13 44 4, 14 44 4,15 44 4, 16 44 4,17 44 4, 18 44 4,19 44 4, 20 44 4, 21 44 4, 22 44 4, 23 44 4, 24 44 4,25 44 4,26 44 4,27 44 4, 28 44 4,29 44 4, 30 44 4, 31 44 4, 32 44 4, 33 44 4, 34 44 4,35 44 4,36 44 4,37 44 4, 38 44 4,39 44 4, 40 44 4, 41 44 4,42 44 4, 43 44 4, 44 44 4, 45 44 4,46 44 4, 4744 4, 48 44 4, 49 44 4, 50 44 4, 51 44 3 , 52 44 4, 53 44 4,54 44 4,11 5, 2 1 5,3 1 5, 4 1 5, 5 1 5, 6 1 5, 7 1 5,8 1 5,9 1 5,10 1 5,11 1 5,12 15,13 1 5,14 1 5,15 1 5,16 1 5,17 1 5,18 1 5, 19 1 5,20 1 5, 21 1 5,22 1 5,23 1 5, 24 1 5,25 1 5, 26 1 5, 27 1 5,28 1 5, 29 1 5,30 1 5, 31 1 5,32 1 5,33 1 5, 34 1 5,35 1 5,36 1 5,371 5, 38 1 5,39 1 5,40 1 5, 41 1 5,42 1 5, 43 1 5,44 1 5,45 1 5,46 1 5,47 1 5,48 1 5,49 1 5, 50 1 5,51 1 3 , 52 1 5,53 1 5,54 1 5, 20 1 2 5, 2 2 5,3 2 5, 4 2 5, 5 2 5,6 2 5,7 2 5, 8 2 5, 9 2 5,10 2 5,11 2 5,12 2 5,13 2 5, 14 2 5, 2 5,16 2 5,17 2 5,18 2 5,19 2 5, 20 2 5, 21 2 5, 22 2 5,23 2 5,24 2 5, 25 2 5,26 2 5, 27 2 5, 28 2 5, 29 2 5,30 2 5,31 2 5,32 2 5,33 2 5, 34 2 5,35 2 5,36 2 5,37 2 5,38 2 5, 39 2 5,40 2 5,41 2 5,42 2 5,43 2 5,44 2 5,45 2 5, 46 2 5,47 2 5,48 2 5, 49 2.5,50 2 5, 51 2 3 ,52 2 5,53 2 5, 54 2 5,1 3 5, 23 5,3 3 5,4 3 5, 5 3 5,6 3 5, 7 3 5, 8 3 5, 9 3 5,10 3 5, 25 11 3 5,12 3 5,13 3 5,14 3 5,15 3 5,16 3 5,17 3 5, 18 3 5,19 3 5,20 3 5, 21 3 5,22 3 5, 23 3 5,24 3 5,25 3 5,26 3 5,27 3 5,28 3 5,29 3 5,30 3 5,31 3 5,32 3 5,33 3 5,34 3 5, 353 5,36 3 5,373 5,38 35,39 3 5,40 3 5,413 5, 4235,4335,4435,4535,4635, 47 3 5,48 3 5, 49 3 5,50 3 5,51 3 3 , 52 3 5,53 3 5, 54 3 5,1 4 5, 2 4 5,3 4 5,4 4 5,5 4 5, 6 4 5,7 4 5,8 4 5, 9 4 5,10 4 5,11 4 5,12 4 5, 13 4 5,14 4 5,15 4 5,16 4 5,17 4 5,18 4 5, 19 4 5,20 4 5, 21 4 5, 22 4 5, 23 4 5, 24 4 5, 25 4 5, 26 4 5,27 4 5,28 4 5, 29 4 5,30 4 5, 31 4 5, 32 4 5,33 4 5,34 4 5,35 4 5,36 4 5, 37 4 5,38 4 5,39 4 5,40 4 5, 41 4 5,42 4 5, 43 4 5,44 4 5,45 4 5,46 4 5,47 4 5,48 4 5, 49 4 5,50 4 5,5143 , 52 4 5,53 4 5,54 4 5, 1 5 5,2 5 5,3 5 5,4 5 5,5 5 5,6 5 5,7 5 5,8 5 5,9 5 5,10 5 5,11 5 5,12 5 5,13 5 5,14 5 5, 15 5 5, 16 5 5,17 5 5,18 5 5,19 5 5, 20 5 5, 21 5 5,22 5 5,23 5 5,24 5 5, 25 5 5,26 5 5, 35 27 5 5,28 5 5,29 5 5,30 5 5,3155,32 5 5,33 5 5,34 5 5,35 5 5,36 5 5,37 5 5,38 5 5, 39 5 5,40 5 5, 415 5, 42 5 5,43 5 5,44 5 5, 45 5 5,46 5 5,47 5 5,4855,4955,5055, 51 5 3 , 52 5 5,53 5 5, 54 5 5,1 6 5, 2 6 5,3 6 5,4 6 5,5 6 5, 6 6 5, 7 6 5, 8 6 5, 9 6 5, 10 6 5, Printed from Mimosa 13 07 15 11 6 5,12 6 5,13 6 5,14 6 5,15 6 5,16 6 5,17 6 5, 18 6 5, 19 6 5, 20 6 5, 21 6 5, 22 6 5, 23 6 5, 24 6 5, 25 6 5, 26 6 5, 27 6 5, 28 6 5, 29 6 5, 30 6 5,31 6 5,32 6 5,33 6 5,34 6 5, 35 6 5, 36 6 5,37 6 5,38 6 5, 39 6 5,40 6 5, 41 6 5,42 6 5,43 6 5, 44 6 5, 45 6 5, 46 6 5, 47 6 5, 48 6 5,49 6 5, 50 6 5, 51 6 3 ,52 6 5,53 6 5, 54 6 5,1 7 5,2 7 5,3 7 5,4 7 5,5 7 5, 5 6 75,7 75,8 7 5, 9 7 5,10 7 5,11 7 5,12 75,13 7 5,14 7 5,15 7 5,16 75,17 75,18 7 5, 19 7 5,20 7 5,21 75,22 7 5,23 7 5,24 7 5,25 7 5, 26 7 5,27 7 5,28 7 5, 29 7 5,30 7 5, 31 7 5,32 7 5, 33 7 5, 34 7 5, 35 7 5, 36 7 5,37 7 5, 38 7 5,39 7 5,40 7 5,41 7 5,42 7 5, 43 7 5,44 7 5, 45 7 5, 46 7 5, 47 7 5, 48 7 5,49 7 5, 50 7 5,51 7 3 ,52 7 5, 53 7 5, 54 7 5, I 8 5,2 8 5,3 8 5, 4 8 5, 5 8 5,6 8 5,7 8 5, 8 8 5, 9 8 5,10 8 5,11 8 5,12 8 5,13 8 5,14 8 5, 10 15 8 5,16 8 5,17 8 5,18 8 5,19 8 5, 20 8 5, 21 8 5, 22 8 5, 23 8 5,24 8 5, 25 8 5,26 8 5, 27 8 5,28 8 5,29 8 5,30 8 5,31 8 5,32 8 5,33 8 5,34 8 5,35 8 5,36 8 5,37 8 5,38 8 5, 39 8 5, 40 8 5,41 8 5,42 8 5,43 8 5,44 8 5, 45 8 5, 46 8 5, 47 8 5,48 8 5,49 8 5,50 8 5, 51 8 3 , 52 8 5,53 8 5,54 8 5,1 9 5,2 9 5,3 9 5, 4 9 5, 5 9 5, 6 9 5, 7 9 5, 8 9 5, 9 9 5,10 9 5, II 9 5,12 9 5,13 9 5,14 9 5,15 9 5,16 9 5,17 9 5, 18 9 5,19 9 5,20 9 5, 21 9 5,22 9 5 15 23 9 5, 24 9 5, 25 9 5, 26 9 5, 27 9 5, 28 9 5, 29 9 5, 30 9 5, 31 9 5,32 9 5, 33 9 5,34 9 5, 9 5,36 9.5,37 9 5,38 9 5,39 9 5,40 9 5,41 9 5, 42 9 5, 43 9 5,44 9 5,45 9 5,46 9 5, 47 9 5,48 9 5,49 9 5,50 9 5,51 9 3,52 9 5,53 9 5,54 9 5,1 10 5,210 5,3 10 5,4 10 5, 5 10 5, 6 10 5,7 10 5,8 10 5, 9 10 5,10 10 5,11 10 5,12 10 5,13 10 5,14 10 5,15 10 5, 16 10 5,17 10 5,18 10 5,19 10 5, 20 10 5,21 10 5, 22 10 5,23 10 5,24 10 5, 25 10 5, 26 10 5,2710 5,28 10 5, 29 10 5,30 10 5, 31 10 5, 32 10 5,33 10 5,34 10 5, 35 10 5, 36 10 5,3710 5,38 10 5,39 10 5,40 10 5, 41 10 5, 42 10 5,43 10 5,44 10 5,45 10 5, 46 10 5,4710 5,48 10 5,49 10 5,50 10 5,51 10 3 ,5210 5,5310 5,54 10 5,111 5,2 11 5, 3 11 5,4 11 5,5 11 5,6 11 5, 7 11 5, 8 11 5,9 11 5,10 11 5,11 11 5,12 11 5,13 11 5, 14 11 5,1511 5,16 11 5,17 11 5,18 11 5,19 11 5, 20 11 5,21 11 5, 22 11 5,23 11 5, 25 24 11 5,25 11 5,26 11 5, 27 11 5, 28 11 5, 29 11 5, 30 11 5,31 11 5,32 11 5,33 11 5, 34 115,3511 5,36 11 5,37 11 5,38 11 5,39 11 5, 40 11 5,41 115,42 11 5,43 11 5, 44 11 5,45115,46 11 5,47 11 5,48 11 5,49 11 5, 50 11 5,51 113,52 11 5,53 11 5, 54 11 5,112 5,2 12 5, 3 12 5,4 12 5, 5 12 5, 6 12 5,712 5,8 12 5, 9 12 5,10 12 5,1112 5, 12 12 5,13 12 5,14 12 5,15 12 5,16 12 5,17 12 5,18 12 5,19 12 5,20 12 5,2112 5, 30 22 12 5,23 12 5, 24 12 5, 25 12 5, 26 12 5, 27 12 5, 28 12 5,29 12 5,30 12 5,31125, 32 12 5,33 12 5,34 12 5,35 12 5, 36 12 5, 37 12 5, 38 12 5,39 12 5,40 12 5,41 12 5, 42 12 5,43 12 5,44 12 5, 45 12 5, 46 12 5,47 12 5, 48 12 5,49 12 5,50 12 5,5112 3 , 52 12 5,53 12 5, 54 12 5,113 5,2 13 5, 3 13 5,4 13 5,5 13 5, 6 13 5,7 13 5, 8 13 5, 9 13 5, 10 13 5,1113 5,12 13 5,13 13 5, 14 13 5,15 13 5,16 13 5,17 13 5,18 13 5,19 13 5, 20 13 5,21 13 5, 22 13 5, 23 13 5, 24 13 5, 25 13 5, 26 13 5, 27 13 5,28 13 5, 29 13 5, 30 13 5,3113 5,32 13 5,33 13 5,34 13 5, 35 13 5,36 13 5,37 13 5,38 13 5,39 13 5, 40 13 5,41 13 5, 42 13 5, 43 13 5, 44 13 5, 45 13 5, 46 13 5,47 13 5,48 13 5, 49 13 5, 26 Printed from Mimosa 13 07 15 50 13 5, 51 13 3 , 52 13 5, 53 13 5,54 13 5,1 14 5, 2 14 5, 3 14 5,4 14 5,5 14 5, 6 14 5, 7 14 5,8 14 5, 9 14 5,10 14 5,11 14 5, 12 14 5,13 14 5, 14 14 5,15 14 5,16 14 5,17 14 5, 18 14 5, 19 14 5, 20 14 5, 21 14 5,22 14 5,23 14 5, 24 14 5, 25 14 5,26 14 5,27 14 5, 28 14 5, 29 14 5, 30 14 5,31 14 5,32 14 5,33 14 5, 34 14 5, 35 14 5,36 14 5,37 14 5, 5 38 14 5, 39 14 5, 40 14 5, 41 14 5,42 14 5,43 14 5,44 14 5,45 14 5,46 14 5,47 14 5, 48 14 5, 49 14 5, 50 14 5, 51 14 3 ,5214 5,5314 5,54 14 5,1 15 5,2 15 5,3 15 5,4 15 5, 5 15 5, 6 15 5, 7 15 5, 8 15 5, 9 15 5,10 15 5,11 15 5,12 15 5,13 15 5,14 15 5,15 15 5, 16 15 5, 17 15 5, 18 15 5,19 15 5,20 15 5,21 15 5,22 15 5,23 15 5,24 15 5,25 15 5, 26 15 5, 27 15 5, 28 15 5, 29 15 5,30 15 5,31 15 5,3215 5,33 15 5,34 15 5,35 15 5, 10 36 15 5, 37 15 5, 38 15 5, 39 15 5, 40 15 5,41 15 5,42 15 5, 43 15 5,44 15 5,45 15 5, 46 15 5, 47 15 5, 48 15 5, 49 15 5,5015 5,51 15 3 ,52 15 5,53 15 5,54 15 5,116 5,2 16 5, 3 16 5,4 16 5, 5 16 5, 6 16 5,7 16 5, 8 16 5, 9 16 5,10 16 5,11 16 5,12 16 5,13 16 5, 14 16 5,15 16 5, 16 16 5,17 16 5,18 16 5,19 16 5, 20 16 5, 21 16 5,22 16 5,23 16 5, 24 16 5, 25 16 5, 26 16 5, 27 16 5, 28 16 5,29 16 5,30 16 5,31 16 5,32 16 5,33 16 5, 15 34 16 5,35 16 5, 36 16 5, 3^ 16 5,38 16 5,39 16 5,40 16 5,41 16 5,42 16 5,43 16 5, 44 16 5, 45 16 5, 46 16 5, 4716 5,48 16 5,49 16 5,50 16 5,5116 3 , 52 16 5,53 16 5, 54 16 5,117 5,2 17 5, 3 17 5, 4 17 5,5 17 5, 6 17 5, 717 5,8 17 5, 9 17 5,10 17 5,11 17 5, 12 17 5,13 17 5, 14 17 5, 15 17 5,16 17 5,17 17 5,18 17 5,19 17 5,20 17 5,21 17 5, 22 17 5, 23 17 5, 24 17 5, 25 17 5, 26 17 5,27 17 5,28 17 5,29 17 5,30 17 5,31 17 5, 20 32 17 5, 33 17 5, 34 17 5, 35 17 5,36 17 5,3717 5,38 17 5,39 17 5,40 17 5,4117 5, 42 17 5, 43 17 5, 44 17 5, 45 17 5,46 17 5,4717 5,48 17 5,49 17 5,50 17 5,51 173, 52 17 5, 53 17 5, 54 17 5, 1 18 5,2 18 5,3 18 5, 4 18 5,5 18 5, 6 18 5,7 18 5,8 18 5, 9 18 5, 10 18 5,11 18 5,12 18 5, 13 18 5,14 18 5,15 18 5,16 18 5,17 18 5,18 18 5,19 18 5, 20 18 5, 21 18 5, 22 18 5, 23 18 5,24 18 5,2518 5,26 18 5, 27 18 5,28 18 5,29 18 5, 25 30 18 5, 31 18 5, 32 18 5, 33 18 5,34 18 5,35 18 5,36 18 5,37 18 5,38 18 5,39 18 5, 40 18 5, 41 18 5, 42 18 5, 43 18 5,44 18 5,45 18 5,46 18 5,47 18 5,48 18 5,49 18 5, 50 18 5, 51 183 ,52 18 5,53 18 5, 54 18 5,1 19 5,2 19 5, 3 19 5,4 19 5, 5 19 5, 6 19 5, 7 19 5,8 19 5, 9 19 5,10 19 5,11 19 5,12 19 5, 13 19 5,14 19 5,15 19 5,16 19 5,17 19 5, 18 19 5,19 19 5, 20 19 5, 2119 5,22 19 5,23 19 5,24 19 5,25 19 5, 26 19 5,27 19 5, 30 28 19 5, 29 19 5,30 19 5, 31 19 5,32 19 5,3319 5,34 19 5,35 19 5,36 19 5,3719 5, 38 19 5, 39 19 5,40 19 5, 4119 5,42 19 5,4319 5,44 19 5,45 19 5,46 19 5,47 19 5, 48 19 5, 49 19 5,50 19 5, 51 19 3, 52 19 5,53 19 5, 54 19 5,1 20 5,2 20 5, 3 20 5, 4 20 5, 5 20 5, 6 20 5,7 20 5, 8 20 5,9 20 5,10 20 5,11 20 5,12 20 5,13 20 5,14 20 5,15 20 5, 16 20 5,17 20 5,18 20 5, 19 20 5,20 20 5,21 20 5,22 20 5,23 20 5,24 20 5,25 20 5, 35 26 20 5, 27 20 5,28 20 5,29 20 5,30 20 5,3120 5,32 20 5,33 20 5,34 20 5,35 20 5, 36 20 5, 37 20 5,38 20 5,39 20 5,40 20 5,41 20 5,42 20 5,43 20 5,44 20 5,45 20 5, 46 20 5, 47 20 5,48 20 5, 49 20 5,50 20 5,51 20 3,52 20 5,53 20 5,54 20 5,1 21 5,2 21 5, 27 Printed from Mimosa 13 07 15 3 21 5, 4 21 5,5 21 5, 6 21 5, 7 21 5, 8 21 5, 9 21 5,10 21 5,11 21 5, 12 21 5,13 21 5, 14 21 5,15 21 5,16 21 5,17 21 5,18 21 5,19 21 5, 20 21 5,21 21 5, 22 21 5,23 21 5, 24 21 5, 25 21 5, 26 21 5,27 21 5, 28 21 5, 29 21 5,30 21 5,31 21 5, 32 21 5,33 21 5, 34 21 5,35 21 5, 36 21 5,37 21 5,38 21 5, 39 21 5,40 21 5,41 21 5, 42 21 5, 43 21 5, 5 44 21 5, 45 21 5, 46 21 5,47 21 5, 48 21 5, 49 21 5, 50 21 5,51 21 3 , 52 21 5, 53 21 5, 54 21 5,1 22 5,2 22 5,3 22 5,4 22 5,5 22 5,6 22 5, 7 22 5, 8 22 5,9 22 5,10 22 5,11 22 5, 12 22 5,13 22 5,14 22 5,15 22 5, 16 22 5, 17 22 5,18 22 5,19 22 5, 20 22 5, 21 22 5, 22 22 5, 23 22 5, 24 22 5,25 22 5, 26 22 5, 27 22 5,28 22 5,29 22 5,30 22 5,31 22 5, 32 22 5,33 22 5,34 22 5,35 22 5,36 22 5,37 22 5,38 22 5,39 22 5, 40 22 5, 41 22 5, 10 42 22 5, 43 22 5,44 22 5,45 22 5, 46 22 5,47 22 5,48 22 5,49 22 5, 50 22 5,51 22 3 , 52 22 5,53 22 5,54 22 5,1 23 5,2 23 5,3 23 5,4 23 5, 5 23 5,6 23 5,7 23 5, 8 23 5, 9 23 5, 10 23 5,11 23 5,12 23 5,13 23 5,14 23 5,15 23 5,16 23 5,17 23 5, 18 23 5,19 23 5, 20 23 5,21 23 5, 22 23 5,23 23 5, 24 23 5, 25 23 5, 26 23 5,27 23 5, 28 23 5, 29 23 5, 30 23 5,31 23 5, 32 23 5,33 23 5,34 23 5,35 23 5,36 23 5,37 23 5, 38 23 5, 39 23 5, 15 40 23 5,41 23 5,42 23 5,43 23 5,44 23 5,45 23 5,46 23 5,47 23 5, 48 23 5,49 23 5, 50 23 5, 51 23 3 , 52 23 5,53 23 5,54 23 5,1 24 5,2 24 5, 3 24 5, 4 24 5,5 24 5, 6 24 5, 7 24 5, 8 24 5,9 24 5, 10 24 5,11 24 5,12 24 5,13 24 5,14 24 5,15 24 5,16 24 5,17 24 5, 18 24 5,19 24 5, 20 24 5,21 24 5,22 24 5, 23 24 5,24 24 5, 25 24 5, 26 24 5, 27 24 5, 28 24 5, 29 245,30 24 5,31 24 5,32 24 5, 33 24 5,34 24 5, 35 24 5, 36 24 5,37 24 5, 20 38 24 5,39 24 5,40 24 5,41 24 5,42 24 5, 43 24 5,44 24 5, 45 24 5,46 24 5,47 24 5, 48 24 5,49 24 5, 50 24 5,51 24 3 ,52 24 5, 53 24 5, 54 24 5, 1 25 5,2 25 5,3 25 5,4 25 5, 5 25 5, 6 25 5,7 25 5, 8 25 5,9 25 5,10 25 5,11 25 5,12 25 5,13 25 5,14 25 5,15 25 5, 16 25 5,17 25 5,18 25 5,19 25 5,20 25 5, 21 25 5, 22 25 5, 23 25 5, 24 25 5,25 25 5, 26 25 5, 27 25 5, 28 25 5,29 25 5,30 25 5,31 25 5,32 25 5,33 25 5, 34 25 5, 35 25 5, 25 36 25 5,37 255,38 25 5,39 25 5,40 25 5, 41 25 5, 42 25 5, 43 25 5, 44 25 5, 45 25 5, 46 25 5,47 25 5,48 25 5,49 25 5,50 25 5, 51 25 3 , 52 25 5, 53 25 5, 54 25 5,1 26 5,2 26 5, 3 26 5,4 26 5,5 26 5, 6 26 5,7 26 5, 8 26 5, 916 5,10 26 5,11 26 5,12 26 5,13 26 5, 14 26 5,15 26 5,16 26 5,17 26 5,18 26 5,19 26 5, 20 26 5, 21 26 5, 22 26 5, 23 26 5, 24 26 5,25 26 5,26 26 5,27 26 5,28 26 5,29 26 5, 30 26 5, 31 26 5, 32 26 5,33 26 5, 30 34 26 5,35 26 5,36 26 5,37 26 5,38 26 5,39 26 5, 40 26 5, 41 26 5, 42 26 5,43 26 5, 44 26 5,45 26 5,46 26 5,47 26 5,48 26 5,49 26 5,50 26 5, 51 26 3 ,52 26 5,53 26 5, 54 26 5,1 27 5,2 27 5,3 27 5,4 27 5,5 27 5, 6 27 5, 7 27 5, 8 27 5,9 27 5,10 27 5,11 27 5, 12 27 5,13 27 5,14 27 5,15 27 5,16 27 5,17 27 5,18 27 5, 19 27 5, 20 27 5, 21 27 5, 22 27 5,23 27 5,24 27 5,25 27 5, 26 27 5, 27 27 5, 28 27 5, 29 27 5,30 27 5,31 27 5, 35 32 27 5,33 27 5,34 27 5,35 27 5,36 27 5,37 27 5, 38 27 5, 39 27 5,40 27 5,41 27 5, 42 27 5,43 27 5,44 27 5,45 27 5, 46 27 5,47 27 5, 48 27 5, 49 27 5, 50 27 5,51 27 3 , 52 27 5,53 27 5,54 27 5,1 28 5,2 28 5,3 28 5,4 28 5,5 28 5, 6 28 5,7 28 5, 8 28 5,9 28 5, 28 Printed from Mimosa 13 07 15 28 5,11 28 5,12 28 5,13 28 5,14 28 5,15 28 5,16 28 5, 17 28 5, 18 28 5,19 28 5, 20 28 5,21 28 5, 22 28 5, 23 28 5,24 28 5,25 28 5, 26 28 5, 27 28 5, 28 28 5,29 28 5, 30 28 5,31 28 5, 32 28 5,33 28 5, 34 28 5,35 28 5, 36 28 5, 37 28 5, 38 28 5,39 28 5, 40 28 5,41 28 5, 42 28 5,43 28 5, 44 28 5,45 28 5,46 28 5,47 28 5,48 28 5,49 28 5, 5 50 28 5,51 28 3 ,52 28 5, 53 28 5, 54 28 5, 1 29 5,2 29 5, 3 29 5, 4 29 5,5 29 5, 6 29 5, 7 29 5, 8 29 5,9 29 5, 10 29 5,11 29 5,12 29 5,13 29 5,14 29 5,15 29 5,16 29 5,17 29 5, 18 29 5,19 29 5, 20 29 5, 21 29 5,22 29 5,23 29 5, 24 29 5, 25 29 5, 26 29 5, 27 29 5, 28 29 5,29 29 5,30 29 5,31 29 5,32 29 5,33 29 5,34 29 5,35 29 5,36 29 5,37 29 5, 38 29 5,39 29 5,40 29 5,41 29 5,42 29 5,43 29 5,44 29 5, 45 29 5, 46 29 5, 47 29 5, 10 48 29 5,49 29 5, 50 29 5,51 29 3 ,52 29 5, 53 29 5, 54 29 5,130 5,2 30 5,3 30 5,4 30 5, 5 30 5, 6 30 5,7 30 5, 8 30 5,9 30 5,10 30 5,11 30 5,12 30 5,13 30 5,14 30 5,15 30 5, 16 30 5,17 30 5,18 30 5,19 30 5, 20 30 5, 21 30 5,22 30 5, 23 30 5, 24 30 5, 25 30 5, 26 30 5,27 30 5, 28 30 5,29 30 5,30 30 5,31 30 5,32 30 5,33 30 5, 34 30 5,35 30 5, 36 30 5,37 30 5,38 30 5,39 30 5,40 30 5,41 30 5,42 30 5,43 30 5,44 30 5, 45 30 5, 15 46 30 5,47 30 5, 48 30 5,49 30 5,50 30 5,51 30 3 , 52 30 5, 53 30 5,54 30 5,1 31 5, 2 31 5, 3 31 5, 4 31 5,5 31 5, 6 31 5,7 31 5, 8 31 5, 9 31 5,10 31 5,11 31 5,12 31 5,13 31 5, 14 31 5,15 31 5,16 31 5,17 31 5,18 31 5,19 31 5,20 31 5, 21 31 5, 22 31 5,23 31 5, 24 31 5,25 31 5, 26 31 5,27 31 5,28 31 5, 29 31 5,30 31 5,31 31 5, 32 31 5,33 315, 34 31 5,35 31 5, 36 31 5,37 31 5,38 31 5,39 31 5,40 31 5,41 31 5, 42 31 5, 43 315, 20 44 31 5,45 31 5,46 31 5,47 31 5,48 31 5,49 31 5, 50 31 5,51 31 3 , 52 31 5, 53 31 5, 54 31 5,1 32 5, 2 32 5,3 32 5,4 32 5, 5 32 5, 6 32 5,7 32 5, 8 32 5,9 32 5,10 32 5,11 32 5, 12 32 5,13 32 5,14 32 5,15 32 5,16 32 5,17 32 5,18 32 5,19 32 5, 20 32 5,21 32 5, 22 32 5,23 32 5,24 32 5,25 32 5,26 32 5,27 32 5, 28 32 5, 29 32 5, 30 32 5,31 32 5, 32 32 5,33 32 5, 34 32 5,35 32 5, 36 32 5,37 32 5,38 32 5,39 32 5, 40 32 5,41 32 5, 25 42 32 5,43 32 5, 44 32 5,45 32 5,46 32 5,47 32 5,48 32 5,49 32 5, 50 32 5,51 32 3 , 52 32 5, 53 32 5, 54 32 5,1 33 5, 2 33 5, 3 33 5,4 33 5, 5 33 5, b 33 5,7 33 5, 8 33 5, 9 33 5, 10 33 5,11 33 5,12 33 5,13 33 5,14 33 5,15 33 5,16 33 5,17 33 5,18 33 5,19 33 5, 20 33 5, 21 33 5, 22 33 5, 23 33 5,24 33 5,25 33 5,26 33 5,27 33 5, 28 335, 29 33 5, 30 33 5,31 33 5, 32 33 5,33 33 5,34 33 5,35 33 5,36 33 5,37 33 5,38 33 5,39 33 5, 30 40 33 5, 41 33 5, 42 33 5,43 33 5,44 33 5,45 33 5,46 33 5,47 33 5,48 33 5, 49 33 5, 50 33 5,51 33 3 , 52 33 5, 53 33 5,54 33 5, 1 34 5,2 34 5, 3 34 5,4 34 5,5 34 5, 6 34 5, 7 34 5, 8 34 5, 9 34 5, 10 34 5,11 34 5,12 34 5,13 34 5,14 34 5,15 34 5,16 34 5,17 34 5, 18 34 5,19 34 5, 20 34 5,21 34 5,22 34 5,23 34 5, 24 34 5,25 34 5, 26 34 5, 27 34 5, 28 34 5, 29 34 5, 30 34 5,31 34 5,32 34 5,33 34 5,34 34 5,35 34 5,36 34 5, 37 34 5, 35 38 34 5, 39 34 5, 40 34 5,41 34 5,42 34 5,43 34 5,44 34 5,45 34 5, 46 34 5, 47 34 5, 48 34 5,49 34 5,50 34 5,51 34 3,52 34 5,53 34 5, 54 34 5,135 5,2 35 5,3 35 5,4 35 5, 5 35 5, 6 35 5,7 35 5, 8 35 5,9 35 5,10 35 5,11 35 5,12 35 5,13 35 5,14 35 5,15 35 5, 29 Printed from Mimosa 13 07 15 16 35 5, 17 35 5, 18 35 5, 19 3 5 5, 20 35 5, 21 35 5,22 35 5, 23 3 5 5,24 35 5, 25 35 5, 26 35 5, 27 35 5, 28 35 5, 29 35 5, 30 35 5, 31 35 5,32 35 5,33 35 5,34 35 5,35 35 5, 36 35 5,37 35 5,38 35 5,39 35 5,40 35 5,41 35 5,42 35 5,43 35 5,44 35 5,45 35 5, 46 35 5, 47 35 5, 48 35 5, 49 35 5,50 35 5, 51 35 3 , 52 35 5,53 35 5,54 35 5, 1 36 5,2 36 5, 5 3 36 5,4 36 5, 5 36 5, 6 36 5, 7 36 5, 8 36 5,9 36 5,10 36 5,11 36 5,12 36 5,13 36 5, 14 36 5,15 36 5,16 36 5,17 36 5,18 36 5,19 36 5,20 36 5,21 36 5,22 36 5,23 36 5, 24 36 5, 25 36 5, 26 36 5, 27 36 5, 28 36 5, 29 36 5,30 36 5,31 36 5,32 36 5,33 36 5, 34 36 5,35 36 5,36 36 5,37 36 5,38 36 5,39 36 5,40 36 5,41 36 5,42 36 5,43 36 5, 44 36 5, 45 36 5, 46 36 5, 47 36 5,48 36 5,49 36 5, 50 36 5,51 36 3 ,52 36 5, 53 36 5, 10 54 36 5,1 37 5,2 37 5, 3 37 5,4 37 5, 5 37 5, 6 37 5, 7 37 5,8 37 5, 9 37 5, 10 37 5,11 37 5, 12 37 5,13 37 5,14 37 5, 15 37 5,16 37 5,17 37 5,18 37 5,19 37 5,20 37 5, 21 37 5, 22 37 5, 23 37 5, 24 37 5, 25 37 5, 26 37 5,27 37 5,28 37 5,29 37 5,30 37 5,31 37 5, 32 37 5, 33 37 5, 34 37 5, 35 37 5,36 37 5,37 37 5,38 37 5,39 37 5,40 37 5,41 37 5, 42 3 7 5, 43 37 5,44 37 5, 45 37 5,46 37 5,47 37 5, 48 37 5,49 37 5,50 37 5, 51 37 3, 15 52 37 5, 53 37 5, 54 37 5,1 38 5,2 38 5,3 38 5,4 38 5,5 38 5, 6 38 5,7 38 5,8 38 5,9 38 5, 10 38 5,11 38 5, 12 38 5,13 38 5,14 38 5,15 38 5,16 38 5,17 38 5,18 38 5,19 38 5, 20 38 5,21 38 5, 22 38 5, 23 38 5, 24 38 5,25 38 5,26 38 5,27 38 5,28 38 5,29 38 5, 30 38 5,31 38 5, 32 38 5, 33 38 5,34 38 5,35 38 5,36 38 5,37 38 5,38 38 5,39 38 5, 40 38 5,41 38 5, 42 38 5, 43 38 5, 44 38 5,45 38 5,46 38 5,47 38 5,48 38 5,49 38 5, 20 50 38 5,51 38 3 ,52 38 5, 53 38 5, 54 38 5,1 39 5,2 39 5, 3 39 5,4 39 5, 5 39 5, 6 39 5, 7 39 5,8 39 5, 9 39 5,10 39 5,11 39 5,12 39 5,13 39 5,14 39 5,15 39 5,16 39 5,17 39 5, 18 39 5,19 39 5,20 39 5, 21 39 5,22 39 5,23 39 5, 24 39 5,25 39 5,26 39 5,27 39 5, 28 39 5, 29 39 5,30 39 5, 31 39 5,32 39 5,33 39 5,34 39 5,35 39 5,36 39 5,37 39 5, 38 39 5, 39 39 5, 40 39 5,41 39 5,42 39 5,43 39 5, 44 39 5,45 39 5,46 39 5,47 39 5, 25 48 39 5,49 39 5, 50 39 5, 51 39 3 ,52 39 5,53 39 5, 54 39 5,1 40 5,2 40 5, 3 40 5, 4 40 5, 5 40 5, 6 40 5,7 40 5,8 40 5,9 40 5,10 40 5,11 40 5,12 40 5,13 40 5,14 40 5,15 40 5, 16 40 5,17 40 5,18 40 5, 19 40 5, 20 40 5,21 40 5, 22 40 5,23 40 5,24 40 5, 25 40 5, 26 40 5,27 40 5, 28 40 5, 29 40 5, 30 40 5,31 40 5,32 40 5,33 40 5,34 40 5,35 40 5, 36 40 5,37 40 5,38 40 5, 39 40 5, 40 40 5,41 40 5,42 40 5,43 40 5,44 40 5,45 40 5, 30 46 40 5, 4740 5,48 40 5, 49 40 5,50 40 5,51 40 3 ,52 40 5,53 40 5,54 40 5,1 41 5,2 41 5, 3 41 5,4 415,5 41 5, 6 41 5,7 41 5,8 41 5,9 41 5,10 41 5,11 41 5,12 41 5,13 41 5, 14 41 5,15 41 5,16 41 5,17 41 5,18 41 5,19 41 5, 20 41 5,21 41 5, 22 41 5, 23 41 5, 24 41 5,25 41 5, 26 41 5, 27 41 5, 28 41 5, 29 41 5, 30 41 5,31 41 5,32 41 5,33 41 5, 34 41 5,35 41 5,36 41 5, 37 41 5,38 41 5,39 41 5,40 41 5,41 41 5,42 41 5,43 41 5, 35 44 41 5,45 41 5,46 41 5,47 41 5, 48 41 5,49 41 5, 50 41 5, 51 41 3 , 52 41 5,53 41 5, 54 41 5,1 42 5, 2 42 5,3 42 5, 4 42 5,5 42 5, 6 42 5,7 42 5, 8 42 5,9 42 5,10 42 5, 11 42 5, 12 42 5,13 42 5,14 42 5, 15 42 5,16 42 5,17 42 5,18 42 5,19 42 5,20 42 5, 21 42 5, Printed from Mimosa 13 07 15 PCT/US96/20Z26 22 42 5, 23 42 5, 24 42 5, 25 42 5, 26 42 5, 27 42 5, 28 42 5,29 42 5,30 42 5,31 42 5, 32 42 5, 33 42 5, 34 42 5, 35 42 5, 36 42 5,37 42 5, 38 42 5,39 42 5,40 42 5, 41 42 5, 42 42 5, 43 42 5, 44 42 5, 45 42 5, 46 42 5, 47 42 5, 48 42 5,49 42 5, 50 42 5,51 42 3 , 52 42 5, 53 42 5, 54 42 5, 1 43 5,2 43 5,3 43 5, 4 43 5, 5 43 5, 6 43 5, 7 43 5, 8 43 5, 9 43 5, 5 10 43 5,11 43 5,12 43 5, 13 43 5,14 43 5,15 43 5,16 43 5,17 43 5,18 43 5,19 43 5, 20 43 5, 21 43 5, 22 43 5, 23 43 5, 24 43 5,25 43 5,26 43 5,27 43 5,28 43 5,29 43 5, 43 5, 31 43 5, 32 43 5, 33 43 5, 34 43 5,35 43 5,36 43 5,37 43 5,38 43 5,39 43 5, 40 43 5, 41 43 5, 42 43 5, 43 43 5, 44 43 5,45 43 5,46 43 5,47 43 5,48 43 5,49 43 5, 50 43 5,51 43 3 ,52 43 5,53 43 5,54 43 5,1 44 5,2 44 5,3 44 5,4 44 5,5 44 5,6 44 5, 10 7 44 5,8 44 5,9 44 5, 10 44 5,11 44 5, 12 44 5,13 44 5,14 44 5,15 44 5,16 44 5,17 44 5, 18 44 5,19 44 5, 20 44 5, 21 44 5, 22 44 5,23 44 5,24 44 5,25 44 5,26 44 5,27 44 5, 28 44 5, 29 44 5, 30 44 5, 31 44 5, 32 44 5,33 44 5,34 44 5,35 44 5,36 44 5,37 44 5, 38 44 5, 39 44 5, 40 44 5, 41 44 5, 42 44 5,43 44 5,44 44 5,45 44 5,46 44 5,47 44 5, 48 44 5, 49 44 5, 50 44 5, 51 44 3 ,52 44 5, 53 44 5,54 44 5, 1 1 6, 2 1 6, 3 1 6,4 1 6, 5 1 6, 15 6 1 6, 7 1 6, 8 1 6,9 1 6,10 1 6,11 1 6, 12 1 6,13 1 6,14 1 6, 15 1 6,16 1 6,17 1 6,18 1 6, 19 1 6, 20 1 6, 21 1 6, 22 1 6,23 1 6, 24 1 6, 25 1 6,26 1 6,27 1 6, 28 1 6, 29 1 6, 30 1 6, 31 1 6,32 1 6,33 1 6, 34 1 6,35 1 6, 36 1 6, 37 1 6,38 1 6,39 1 6( 40 1 6,41 1 6,42 1 6, 43 1 6,44 1 6,45 1 6, 46 1 6,471 6,48 1 6, 49 1 6,50 1 6,51 1 3 , 52 1 6,53 1 6, 54 1 6, I 2 6, 2 2 6,3 2 6,4 2 6,5 2 6, 6 2 6, 7 2 6,8 2 6, 9 2 6,10 2 6,11 2 6,12 2 6,13 2 6,14 2 6, 20 15 2 6,16 2 6,17 2 6,18 2 6,19 2 6, 20 2 6, 21 2 6,22 2 6, 23 2 6, 24 2 6, 25 2 6, 26 2 6, 27 2 6, 28 2 6,29 2 6,30 2 6,31 2 6, 32 2 6,33 2 6,34 2 6,35 2 6, 36 2 6,37 2 6, 38 2 6, 39 2 6, 40 2 6,41 2 6,42 2 6,43 2 6, 44 2 6,45 2 6,46 2 6, 47 2 6, 48 2 6, 49 2 6, 50 2 6, 51 2 3 , 52 2 6,53 2 6, 54 2 6,1 3 6, 2 3 6,3 3 6,4 3 6,5 3 6, 6 3 6, 7 3 6, 8 3 6, 9 3 6,10 3 6, II 3 6,12 3 6,13 3 6,14 3 6,15 3 6, 16 3 6,17 3 6,18 3 6,19 3 6, 20 3 6,21 3 6, 22 3 6, 25 23 3 6,24 3 6,25 3 6,26 3 6,27 3 6, 28 3 6, 29 3 6,30 3 6, 31 3 6,32 3 6,33 3 6,34 3 6, 3 6,36 3 6, 37 3 6, 38 3 6,39 3 6, 40 3 6, 41 3 6, 42 3 6, 43 3 6, 44 3 6, 45 3 6, 46 3 6, 47 3 6,48 3 6, 49 3 6, 50 3 6,51 3 3 ,52 3 6, 53 3 6, 54 3 6,1 4 6,2 4 6,3 4 6, 4 4 6, 5 4 6, 6 4 6, 7 4 6,8 4 6, 9 4 6,10 4 6,11 4 6, 12 4 6, 13 4 6,14 4 6, 15 4 6,16 4 6, 17 4 6,18 4 6, 19 4 6, 20 4 6, 21 4 6, 22 4 6,23 4 6, 24 4 6, 25 4 6,26 4 6, 27 4 6, 28 4 6, 29 4 6, 30 4 6, 30 31 4 6,32 4 6,33 4 6,34 4 6,35 4 6, 36 4 6,37 4 6,38 4 6, 39 4 6, 40 4 6, 41 4 6, 42 4 6, 43 4 6,44 4 6,45 4 6,46 4 6,47 4 6,48 4 6,49 4 6,50 4 6, 51 4 3 , 52 4 6,53 4 6,54 4 6, I 5 6, 2 5 6, 3 5 6, 4 5 6,5 5 6, 6 5 6, 7 5 6,8 5 6, 9 5 6, 10 5 6,11 5 6,12 5 6,13 5 6,14 5 6, 15 5 6,16 5 6,17 5 6,18 5 6,19 5 6, 20 5 6, 21 5 6, 22 5 6, 23 5 6, 24 5 6, 25 5 6, 26 5 6, 27 5 6, 28 5 6, 29 5 6,30 5 6,31 5 6, 32 5 6,33 5 6,34 5 6, 35 5 6,36 5 6,37 5 6, 38 5 6, 35 39 5 6, 40 5 6,41 5 6,42 5 6,43 5 6, 44 5 6, 45 5 6,46 5 6, 47 5 6, 48 5 6, 49 5 6, 50 5 6, 51 5 3 ,52 5 6,53 5 6,54 5 6,1 6 6, 2 6 6,3 6 6,4 6 6, 5 6 6, 6 6 6,7 6 6, 8 6 6, 9 6 6,10 6 6, II 6 6,12 6 6,13 6 6, 14 6 6,15 6 6, 16 6 6,17 6 6,18 6 6,19 6 6, 20 6 6, 2 1 6 6, 22 6 6, 31 Printed from Mimosa 13 07 15 23 6 6,24 6 6, 25 6 6,26 6 6,27 6 6, 28 6 6, 29 6 6, 30 6 6,31 6 6, 32 6 6,33 6 6, 34 6 6, 35 6 6, 36 6 6, 37 6 6, 38 6 6,39 6 6,40 6 6, 41 6 6,42 6 6, 43 6 6,44 6 6, 45 6 6, 46 6 6, 47 6 6,48 6 6,49 6 6, 50 6 6,51 6 3 , 52 6 6, 53 6 6,54 6 6,1 7 6, 2 7 6,3 7 6, 4 7 6, 5 7 6, 6 7 6, 7 7 6, 8 7 6, 9 7 6,10 7 6, 11 7 6, 12 7 6,13 7 6,14 7 6,15 7 6,16 7 6,17 7 6, 18 7 6, 19 7 6, 20 7 6,21 7 6,227 6,23 7 6,24 7 6, 25 7 6,26 7 6,27 7 6, 28 7 6, 29 7 6, 30 7 6, 31 7 6,32 7 6,33 7 6, 34 7 6,35 7 6, 36 7 6,37 7 6,38 7 6,39 7 6, 40 7 6,41 7 6, 42 7 6, 43 7 6,44 7 6, 45 7 6, 46 7 6,47 7 6,48 7 6,49 7 6,50 7 6, 51 7 3 ,52 7 6,53 7 6, 54 7 6, I 8 6,2 8 6,3 8 6, 4 8 6,5 8 6,6 8 6,7 8 6,8 8 6,9 8 6,10 8 6,11 8 6,12 8 6,13 8 6,14 8 6, 8 6,16 8 6,17 8 6, 18 8 6,19 8 6,20 8 6, 21 8 6, 22 8 6, 23 8 6, 24 8 6,25 8 6, 26 8 6, 27 8 6, 28 8 6,29 8 6,30 8 6,31 8 6,32 8 6, 33 8 6,34 8 6,35 8 6,36 8 6,37 8 6, 38 8 6, 39 8 6,40 8 6,41 8 6, 42 8 6,43 8 6,44 8 6, 45 8 6,46 8 6,47 8 6,48 8 6,49 8 6, 50 B 6, 51 8 3 , 52 8 6,53 8 6,54 8 6,1 9 6, 2 9 6,3 9 6,4 9 6,5 9 6, 6 9 6, 7 9 6, 8 9 6,9 9 6,10 9 6, II 9 6,12 9 6,13 9 6,14 9 6,15 9 6,16 9 6,17 9 6,18 9 6,19 9 6, 20 9 6,21 9 6,22 9 6, 23 9 6,24 9 6,25 9 6,26 9 6,27 9 6,28 9 6, 29 9 6,30 9 6,31 9 6,32 9 6,33 9 6,34 9 6, 9 6,36 9 6,37 9 6,38 9 6,39 9 6,40 9 6, 41 9 6,42 9 6,43 9 6,44 9 6,45 9 6, 46 9 6, 47 9 6,48 9 6,49 9 6, 50 9 6,51 9 3 , 52 9 6, 53 9 6,54 9 6,1 10 6,2 10 6,3 10 6,4 10 6, 5 10 6, 6 10 6,7 10 6, 8 10 6, 9 10 6,10 10 6,11 10 6,12 10 6,13 10 6,14 10 6,15 10 6, 16 10 6,17 10 6,18 10 6,19 10 6,20 10 6,21 10 6, 22 10 6, 23 10 6, 24 10 6, 25 10 6, 26 10 6,27 10 6, 28 10 6, 29 10 6,30 10 6,31 10 6, 32 10 6, 33 10 6, 34 10 6,35 10 6, 36 10 6, 37 10 6,38 10 6,39 10 6,40 10 6,41 10 6, 42 10 6, 43 10 6,44 10 6, 45 10 6, 46 10 6,47 10 6,48 10 6,49 10 6, 50 10 6,51 10 3 ,52 10 6, 53 10 6, 54 10 6,1 11 6, 2 11 6, 3 11 6, 4 11 6,5 11 6, 6 11 6,7 11 6, 8 11 6, 9 11 6,10 11 6,11 11 6, 12 11 6,13 11 6, 14 11 6,15 11 6,16 11 6,17 11 6,18 11 6,19 11 6,20 11 6, 21 11 6, 22 11 6, 23 11 6, 24 11 6,25 11 6,26 11 6,2711 6,28 11 6,29 11 6,30 11 6,31 11 6, 32 11 6, 33 11 6, 34 11 6,35 11 6,36 11 6,37 11 6,38 11 6,39 11 6,40 11 6,41 11 6,42 11 6, 43 11 6, 44 11 6,45 11 6,46 11 6,47 11 6,48 11 6,49 11 6,50 11 6,51 11 3 ,5211 6,53 11 6, 54 11 6,1 12 6, 2 12 6,3 12 6,4 12 6, 5 12 6,6 12 6, 7 12 6, 8 12 6,9 12 6, 10 12 6,11 12 6, 12 12 6,13 12 6,14 12 6,15 12 6,16 12 6,17 12 6,18 12 6,19 12 6, 20 12 6, 21 12 6, 22 12 6,23 12 6,24 12 6,25 12 6, 26 12 6, 27 12 6, 28 12 6, 29 12 6, 30 12 6,31 12 6, 32 12 6,33 12 6,34 12 6,35 12 6, 36 12 6,37 12 6,38 12 6,39 12 6, 40 12 6, 41 12 6, 42 12 6,43 12 6,44 12 6,45 12 6,46 12 6,47 12 6,48 12 6,49 12 6,50 12 6,5112 3, 52 12 6, 53 12 6, 54 12 6,1 13 6,2 13 6, 3 13 6,4 13 6, 5 13 6, 6 13 6,7 13 6, 8 13 6, 9 13 6, 10 13 6,11 13 6,12 13 6,13 13 6,14 13 6,15 13 6,16 13 6,17 13 6,18 13 6,19 13 6, 13 6, 21 13 6, 22 13 6, 23 13 6, 24 13 6, 25 13 6,26 13 6, 27 13 6, 28 13 6, 29 13 6, 30 13 6, 31 13 6,32 13 6,33 13 6,34 13 6, 35 13 6,36 13 6,37 13 6,38 13 6, 39 13 6, 40 13 6,41 13 6,42 13 6,43 13 6,44 13 6,45 13 6,46 13 6,47 13 6,48 13 6, 49 13 6, 50 13 6, 51 13 3 , 52 13 6, 53 13 6, 54 13 6,1 14 6,2 14 6,3 14 6,4 14 6,5 14 6, 6 14 6, 32 Printed from Mimosa 13 07 15 PCT/US96/20Z26 7 14 6,8 14 6,9 14 6,10 14 6,11 14 6,12 14 6,13 14 6,14 14 6,15 14 6,16 14 6,17 14 6, 18 14 6,19 14 6,20 14 6, 21 14 6,22 14 6,23 14 6,24 14 6, 25 14 6, 26 14 6, 27 14 6, 28 14 6,29 14 6,30 14 6,31 14 6,32 14 6,33 14 6,34 14 6,35 14 6, 36 14 6, 37 14 6, 38 14 6,39 14 6,40 14 6,41 14 6,42 14 6,43 14 6,44 14 6,45 14 6, 46 14 6,47 14 6, 5 48 14 6,49 14 6,50 14 6,51 14 3 ,52 14 6,5314 6, 54 14 6,1 15 6,2 15 6,3 15 6,4 15 6, 5 15 6/ 6 15 6,7 15 6, 8 15 6,9 15 6,10 15 6,11 15 6,12 15 6,13 15 6,14 15 6,15 15 6, 16 15 6,17 15 6,18 15 6,19 15 6,20 15 6,2115 G, 22 15 6, 23 15 6, 24 15 6,25 15 6, 26 15 6,2715 6,28 15 6,29 15 6,30 15 6,31 15 6,32 15 6, 33 15 6,34 15 6,35 15 6, 36 15 6,3715 6,38 15 6,39 15 6,40 15 6,4115 6,42 15 6, 43 15 6,44 15 6,45 15 6, 10 46 15 6,4715 6,48 15 6,49 15 6,50 15 6,51 15 3 ,52 15 6,53 15 6,54 15 6,1 16 6,2 16 6, 3 16 6,4 16 6,516 6, 6 16 6,716 6,8 16 6, 9 16 6,10 16 6, 11 16 6,12 16 6,13 16 6, 14 16 6,15 16 6,16 16 6,17 16 6,18 16 6,19 16 6,20 16 6, 21 16 6, 22 16 6,23 16 6, 24 16 6,25 16 6, 26 16 6,27 16 6,28 16 6, 29 16 6,30 16 6, 31 16 6, 32 16 6,33 16 6, 34 16 6,35 16 6,36 16 6,37 16 6,38 16 6,39 16 6,40 16 6, 41 16 6, 42 16 6,43 16 6, 15 44 16 6,45 16 6,46 16 6,47 16 6,48 16 6,49 16 6,50 16 6, 51 16 3 , 52 16 6, 53 16 6, 54 16 6,1 17 6,2 17 6,3 1 7 6, 4 17 6, 5 17 6,6 17 6,7 17 6, 8 17 6,9 17 6,10 17 6,1117 6, 12 17 6,13 17 6,14 17 6,15 17 6,16 17 6,1717 6,18 17 6,19 17 6, 20 17 6,21 17 6, 22 17 6,23 17 6,24 17 6,25 17 6,26 17 6,2717 6,28 17 6,29 17 6, 30 17 6,31 17 6, 32 17 6,33 17 6,34 17 6,35 17 6,36 17 6,3717 6,38 17 6, 39 17 6,40 17 6,41 17 6, 20 42 17 6,43 17 6,44 17 6,45 17 6,46 17 6,4717 6,48 17 6,49 17 6, 50 17 6,51 17 3 , 52 17 6,53 17 6,54 17 6,1 18 6,2 18 6,3 18 6,4 18 6,5 18 6,6 18 6,7 18 6,8 18 6,9 18 6, 10 18 6,11 18 6,12 18 6,13 18 6,14 18 6,15 18 6,16 18 6, 17 18 6,18 18 6,19 18 6, 20 18 6,2118 6,22 18 6,23 18 6,24 18 6,25 18 6,26 18 6,27 18 6, 28 18 6,29 18 6, 30 18 6,31 18 6,32 18 6,33 18 6,34 18 6,35 18 6,36 18 6, 37 18 6, 38 18 6,39 18 6, 25 40 18 6,41 18 6,42 18 6,43 18 6,44 18 6,45 18 6,46 18 6, 47 18 6, 48 18 6, 49 18 6, 50 18 6,51 18 3 ,52 18 6, 53 18 6,54 18 6,1 19 6, 2 19 6,3 19 6,4 19 6,5 19 6, 6 19 6, 7 19 6,8 19 6,9 19 6, 10 19 6,11 19 6, 12 19 6,13 19 6,14 19 6,15 19 6,16 19 6,17 19 6, 18 19 6,19 19 6,2019 6,2119 6,22 19 6,2319 6,24 19 6, 25 19 6,26 19 6,27 19 6, 28 19 6,29 19 6,30 19 6,31 19 6,32 19 6,33 19 6,34 19 6,35 19 6,36 19 6,37 19 6, 30 38 19 6,39 19 6,40 19 6,41 19 6,42 19 6,43 19 6,44 19 6, 45 19 6,46 19 6,47 19 6, 48 19 6,49 19 6,50 19 6,51 19 3 ,52 19 6,5319 6,54 19 6,1 20 6,2 20 6, 3 20 6,4 20 6, 5 20 6, 6 20 6,7 20 6, 8 20 6,9 20 6,10 20 6,11 20 6,12 20 6,13 20 6,14 20 6,15 20 6, 16 20 6,17 20 6,18 20 6,19 20 6,20 20 6,21 20 6,22 20 6, 23 20 6, 24 20 6,25 20 6, 26 20 6,27 20 6, 28 20 6,29 20 6,30 20 6,31 20 6,32 20 6,33 20 6,34 20 6,35 20 6, 35 36 20 6,37 20 6,38 20 6,39 20 6,40 20 6,41 20 6,42 20 6, 43 20 6,44 20 6,45 20 6, 46 20 6,47 20 6,48 20 6,49 20 6, 50 20 6,51 20 3 ,52 20 6,53 20 6, 54 20 6,1 21 6,2 21 6, 3 21 6,4 21 6,5 21 6, 6 21 6,7 21 6, 8 21 6,9 21 6,10 21 6, 11 21 6,12 21 6,13 21 6, 33 Printed from Mimosa 13 07 15 14 21 6,15 21 6,16 21 6,17 21 6,18 21 6, 19 21 6, 20 21 6, 21 21 6, 22 21 6, 23 21 6, 24 21 6, 25 21 6,26 21 6,27 21 6,28 21 6, 29 21 6, 30 21 6,31 21 6,32 21 6,33 21 6, 34 21 6,35 21 6,36 21 6,37 21 6,38 21 6, 39 21 6, 40 21 6, 41 21 6, 42 21 6,43 21 6, 44 21 6,45 21 6,46 21 6,47 21 6,48 21 6, 49 21 6, 50 21 6,51 21 3 ,52 21 6, 53 21 6, 54 21 6,1 22 6, 2 22 6,3 22 6, 4 22 6,5 22 6, 6 22 6, 7 22 6, 8 22 6,9 22 6,10 22 6,11 22 6, 12 22 6,13 22 6,14 22 6,15 22 6,16 22 6,17 22 6, 18 22 6,19 22 6, 20 22 6, 21 22 6, 22 22 6,23 22 6,24 22 6, 25 22 6, 26 22 6,27 22 6,28 22 6, 29 22 6,30 22 6,31 22 6, 32 22 6,33 22 6,34 22 6,35 22 6,36 22 6,37 22 6,38 22 6,39 22 6,40 22 6,41 22 6, 42 22 6,43 22 6,44 22 6,45 22 6,46 22 6, 47 22 6,48 22 6,49 22 6,50 22 6,51 22 3 , 52 22 6, 53 22 6,54 22 6,1 23 6, 2 23 6,3 23 6,4 23 6,5 23 6, 6 23 6, 7 23 6,8 23 6, 9 23 6, 10 23 6,11 23 6,12 23 6,13 23 6,14 23 6,15 23 6,16 23 6,17 23 6,18 23 6,19 23 6, 20 23 6,21 23 6, 22 23 6, 23 23 6,24 23 6, 25 23 6, 26 23 6, 27 23 6, 28 23 6,29 23 6, 30 23 6,31 23 6,32 23 6,33 23 6,34 23 6,35 23 6,36 23 6,37 23 6,38 23 6,39 23 6, 40 23 6,41 23 6,42 23 6, 43 23 6,44 23 6,45 23 6,46 23 6, 47 23 6,48 23 6,49 23 6, 50 23 6, 51 23 3 , 52 23 6,53 23 6,54 23 6,1 24 6, 2 24 6,3 24 6, 4 24 6,5 24 6, 6 24 6, 7 24 6,8 24 6,9 24 6,10 24 6,11 24 6, 12 24 6,13 24 6, 14 24 6,15 24 6,16 24 6,17 24 6, 18 24 6,19 24 6,20 24 6,21 24 6,22 24 6,23 24 6,24 24 6, 25 24 6, 26 24 6,27 24 6, 28 24 6,29 24 6,30 24 6,31 24 6,32 24 6,33 24 6,34 24 6,35 24 6,36 24 6,37 24 6, 38 24 6, 39 24 6,40 24 6,41 24 6,42 24 6,43 24 6,44 24 6,45 24 6, 46 24 6,47 24 6, 48 24 6, 49 24 6,50 24 6,51 24 3, 52 24 6,53 24 6,54 24 6,1 25 6,2 25 6, 3 25 6,4 25 6, 5 25 6,6 25 6,7 25 6, 8 25 6,9 25 6,10 25 6,11 25 6,12 25 6,13 25 6,14 25 6,15 25 6, 16 25 6,17 25 6,18 25 6,19 25 6,20 25 6, 21 25 6, 22 25 6, 23 25 6, 24 25 6, 25 25 6, 26 25 6,27 25 6,28 25 6,29 25 6,30 25 6,31 25 6,32 25 6,33 25 6,34 25 6,35 25 6, 36 25 6, 37 25 6,38 25 6,39 25 6,40 25 6,41 25 6,42 25 6,43 25 6,44 25 6, 45 25 6, 46 25 6,47 25 6,48 25 6,49 25 6,50 25 6,51 25 3 ,52 25 6,53 25 6,54 25 6,1 26 6,2 26 6, 3 26 6,4 26 6,5 26 6, 6 26 6,7 26 6,8 26 6,9 26 6,10 26 6,11 26 6,12 26 6,13 26 6, 14 26 6,15 26 6,16 26 6,17 26 6,18 26 6,19 26 6,20 26 6, 21 26 6,22 26 6, 23 26 6, 24 26 6, 25 26 6,26 26 6, 27 26 6,28 26 6,29 26 6,30 26 6,31 26 6,32 26 6,33 26 6, 34 26 6,35 26 6,36 26 6,37 26 6,38 26 6,39 26 6,40 26 6,41 26 6,42 26 6,43 26 6, 44 26 6,45 26 6,46 26 6,47 26 6,48 26 6,49 26 6,50 26 6, 51 26 3 , 52 26 6, 53 26 6, 54 26 6,1 27 6, 2 27 6,3 27 6, 4 27 6,5 27 6,6 27 6,7 27 6,8 27 6,9 27 6,10 27 6, 11 27 6, 12 27 6,13 27 6,14 27 6,15 27 6,16 27 6,17 27 6,18 27 6,19 27 6, 20 27 6, 21 27 6, 22 27 6, 23 27 6,24 27 6,25 27 6,26 27 6,27 27 6, 28 27 6, 29 27 6, 30 27 6, 31 27 6, 32 27 6, 33 27 6,34 27 6,35 27 6,36 27 6,37 27 6,38 27 6,39 27 6, 40 27 6, 41 27 6, 42 27 6,43 27 6,44 27 6,45 27 6,46 27 6,47 27 6,48 27 6,49 27 6, 50 27 6, 51 27 3 , 52 27 6,53 27 6,54 27 6,1 28 6,2 28 6,3 28 6,4 28 6,5 28 6,6 28 6,7 28 6,8 28 6,9 28 6, 10 28 6, 11 28 6,12 28 6,13 28 6,14 28 6,15 28 6,16 28 6,17 28 6,18 28 6,19 28 6, 34 Printed from Mimosa 13 07 15 28 6,21 28 6, 22 28 6, 23 28 6, 24 28 6, 25 28 6,26 28 6, 27 28 6,28 28 6, 29 28 6, 30 28 6,31 28 6,32 28 6,33 28 6,34 28 6,35 28 6,36 28 6,37 28 6,38 28 6,39 28 6, 40 28 6, 41 28 6, 42 28 6, 43 28 6, 44 28 6, 45 28 6,46 28 6; 47 28 6, 48 28 6, 49 28 6, 50 28 6, 51 28 3 ,52 28 6, 53 28 6,54 28 6, 1 29 6,2 29 6, 3 29 6,4 29 6, 5 29 6, 6 29 6, 5 7 29 6, 8 29 6, 9 29 6,10 29 6,11 29 6,12 29 6,13 29 6,14 29 6,15 29 6,16 29 6,17 29 6, 18 29 6,19 29 6, 20 29 6 21 29 6, 22 29 6, 23 29 6,24 29 6, 25 29 6,26 29 6,27 29 6, 28 29 6,29 29 6,30 29 6,31 29 6,32 29 6,33 29 6,34 29 6,35 29 6,36 29 6, 37 29 6, 38 29 6,39 29 6, 40 29 6,41 29 6,42 29 6,43 29 6,44 29 6,45 29 6,46 29 6, 47 29 6, 48 29 6,49 29 6, 50 29 6, 51 29 3 , 52 29 6, 53 29 6, 54 29 6,130 6,2 30 6,3 30 6,4 30 6, 10 5 30 6, 6 30 6, 7 30 6, 8 30 6,9 30 6,10 30 6,11 30 6,12 30 6,13 30 6,14 30 6,15 30 6, 16 30 6,17 30 6,18 30 6,19 30 6, 20 30 6, 21 30 6,22 30 6,23 30 6,24 30 6, 25 30 6, 26 30 6,27 30 6, 28 30 6, 29 30 6, 30 30 6,31 30 6,32 30 6,33 30 6,34 30 6,35 30 6, 36 30 6,37 30 6, 38 30 6,39 30 6, 40 30 6, 41 30 6,42 30 6,43 30 6, 44 30 6,45 30 6, 46 30 6,47 30 6, 48 30 6, 49 30 6, 50 30 6, 51 30 3 , 52 30 6,53 30 6,54 30 6,1 31 6, 2 31 6, 15 3 31 6, 4 31 6,5 31 6, 6 31 6,7 31 6,8 31 6,9 31 6,10 31 6,11 31 6,12 31 6,13 31 6, 14 31 6,15 31 6,16 31 6,17 31 6,18 31 6,19 31 6,20 31 6, 21 31 6, 22 31 6, 23 31 6, 24 31 6,25 31 6, 26 31 6, 27 31 6, 28 31 6, 29 31 6,30 31 6, 31 31 6,32 31 6,33 31 6, 34 31 6,35 31 6,36 31 6, 37 31 6,38 31 6, 39 31 6,40 31 6,41 31 6, 42 31 6,43 31 6, 44 31 6,45 31 6,46 31 6,47 31 6, 48 31 6,49 31 6, 50 31 6,51 31 3 , 52 31 6, 53 31 6, 20 54 31 6,1 32 6,2 32 6, 3 32 6, 4 32 6, 5 32 6, 6 32 6, 7 32 6, 8 32 6,9 32 6,10 32 6,11 32 6, 12 32 6,13 32 6,14 32 6,15 32 6,16 32 6,17 32 6,18 32 6,19 32 6, 20 32 6,21 32 6, 22 32 6, 23 32 6,24 32 6, 25 32 6,26 32 6, 27 32 6, 28 32 6,29 32 6, 30 32 6,31 32 6, 32 32 6,33 32 6,34 32 6,35 32 6,36 32 6,37 32 6,38 32 6,39 32 6, 40 32 6,41 32 6, 42 32 6,43 32 6, 44 32 6, 45 32 6,46 32 6, 47 32 6,48 32 6,49 32 6, 50 32 6,51 32 3 , 25 52 32 6,53 32 6, 54 32 6,1 33 6, 2 33 6,3 33 6,4 33 6, 5 33 6, 6 33 6,7 33 6,8 33 6,9 33 6, 10 33 6,11 33 6,12 33 6,13 33 6,14 33 6,15 33 6,16 33 6,17 33 6,18 33 6,19 33 6, 20 33 6, 21 33 6, 22 33 6, 23 33 6, 24 33 6, 25 33 6, 26 33 6,27 33 6, 28 33 6,29 33 6, 30 33 6,31 33 6,32 33 6,33 33 6, 34 33 6,35 33 6,36 33 6,37 33 6,38 33 6,39 33 6, 40 33 6,41 33 6, 42 33 6,43 33 6,44 33 6,45 33 6, 46 33 6,47 33 6, 48 33 6,49 33 6, 30 50 33 6,51 33 3 , 52 33 6,53 33 6,54 33 6, 1 34 6,2 34 6,3 34 6,4 34 6, 5 34 6,6 34 6, 7 34 6, 8 34 6,9 34 6,10 34 6,11 34 6,12 34 6,13 34 6,14 34 6,15 34 6,16 34 6,17 34 6, 18 34 6,19 34 6, 20 34 6, 21 34 6,22 34 6, 23 34 6,24 34 6,25 34 6,26 34 6,27 34 6, 28 34 6,29 34 6,30 34 6, 31 34 6,32 34 6, 33 34 6,34 34 6,35 34 6,36 34 6,37 34 6, 38 34 6,39 34 6,40 34 6, 41 34 6,42 34 6, 43 34 6, 44 34 6,45 34 6,46 34 6,47 34 6, 35 48 34 6,49 34 6,50 34 6, 51 34 3 ,52 34 6,53 34 6, 54 34 6, 1 35 6, 2 35 6,3 35 6,4 35 6, 5 35 6, 6 35 6, 7 35 6, 8 35 6, 9 35 6,10 35 6, 11 35 6,12 35 6,13 35 6,14 35 6,15 35 6, 16 35 6,17 35 6,18 35 6,19 35 6, 20 35 6, 21 35 6, 22 35 6,23 35 6,24 35 6,25 35 6, Printed from Mimosa 13 07 15 26 35 6, 27 35 6, 28 35 6, 29 35 6, 30 35 6,31 35 6,32 35 6, 33 35 6, 34 35 6,35 35 6, 36 35 6, 37 35 6, 38 35 6, 39 35 6, 40 35 6, 41 35 6, 42 35 6, 43 35 6,44 35 6, 45 35 6 46 35 6,47 35 6, 48 35 6, 49 35 6, 50 35 6, 51 35 3 ,52 35 6,53 35 6, 54 35 6,1 36 6, 2 36 6, 3 36 6,4 36 6, 5 36 6,6 36 6, 7 36 6,8 36 6, 9 36 6, 10 36 6,11 36 6,12 36 6,13 36 6, 14 36 6,15 36 6,16 36 6,17 36 6,18 36 6,19 36 6, 20 36 6,21 36 6,22 36 6,23 36 6, 24 36 6, 25 36 6, 26 36 6, 27 36 6, 28 36 6, 29 36 6,30 36 6,31 36 6,32 36 6,33 36 6, 34 36 6,35 36 6,36 36 6, 37 36 6,38 36 6, 39 36 6,40 36 6, 41 36 6,42 36 6,43 36 6, 44 36 6,45 36 6, 46 36 6, 47 36 6,48 36 6,49 36 6, 50 36 6,51 36 3 , 52 36 6,53 36 6, 54 36 6,1 37 6,2 37 6, 3 37 6,4 37 6, 5 37 6,6 37 6,7 37 6,8 37 6, 9 37 6, 10 37 6,11 37 6, 12 37 6,13 37 6,14 37 6, 15 37 6,16 37 6,17 37 6,18 37 6,19 37 6, 20 37 6, 21 37 6, 22.37 6, 23 37 6, 24 37 6, 25 37 6,26 37 6,27 37 6,28 37 6,29 37 6,30 37 6,31 37 6, 32 37 6, 33 37 6,34 37 6, 35 37 6,36 37 6,37 37 6,38 37 6,39 37 6,40 37 6,41 37 6, 42 37 6,43 37 6, 44 37 6, 45 37 6,46 37 6, 47 37 6,48 37 6,49 37 6, 50 37 6, 51 37 3 , 52 37 6, 53 37 6, 54 37 6,1 38 6, 2 38 6,3 38 6,4 38 6, 5 38 6, 6 38 6,7 38 6, 8 38 6,9 38 6, 10 38 6,11 38 6,12 38 6,13 38 6,14 38 6,15 38 6,16 38 6,17 38 6,18 38 6,19 38 6, 20 38 6, 21 38 6, 22 38 6, 23 38 6, 24 38 6, 25 38 6, 26 38 6, 27 38 6,28 38 6,29 38 6, 30 38 6,31 38 6, 32 38 6, 33 38 6, 34 38 6,35 38 6,36 38 6,37 38 6, 38 38 6,39 38 6, 40 38 6,41 38 6,42 38 6, 43 38 6,44 38 6,45 38 6,46 38 6,47 38 6,48 38 6,49 38 6, 50 38 b, 51 38 3 ,52 38 6, 53 38 6, 54 38 6,1 39 6,2 39.6, 3 39 6,4 39 6, 5 39 6, 6 39 6, 7 39 6r 8 39 6,9 39 6,10 39 6,11 39 6,12 39 6,13 39 6,14 39 6,15 39 6,16 39 6,17 39 6, 18 39 6,19 39 6, 20 39 6, 21 39 6, 22 39 6,23 39 6, 24 39 6,25 39 6, 26 39 6,27 39 6, 28 39 6, 29 39 6, 30 39 6, 31 39 6, 32 39 6, 33 39 6,34 39 6,35 39 6, 36 39 6,37 39 6, 38 39 6, 39 39 6, 40 39 6,41 39 6,42 39 6,43 39 6, 44 39 6,45 39 6, 46 39 6,47 39 6, 48 39 6,49 39 6, 50 39 6, 51 39 3 , 52 39 6, 53 39 6,54 39 6,1 40 6, 2 40 6,3 40 6,4 40 6, 5 40 6,6 40 6,7 40 6, 8 40 6,9 40 6,10 40 6,11 40 6,12 40 6,13 40 6,14 40 6, 15 40 6, 16 40 6,17 40 6,18 40 6, 19 40 6, 20 40 6, 21 40 6,22 40 6, 23 40 6, 24 40 6, 25 40 6, 26 40 6, 27 40 6, 28 40 6, 29 40 6,30 40 6,31 40 6,32 40 6,33 40 6,34 40 6,35 40 6, 36 40 6,37 40 6,38 40 6, 39 40 6, 40 40 6,41 40 6,42 40 6,43 40 6,44 40 6, 45 40 6. 46 40 6, 47 40 6, 48 40 6, 49 40 6, 50 40 6, 51 40 3 ,52 40 6,53 40 6,54 40 6,1 41 6, 2 41 6, 3 41 6,4 41 6, 5 41 6, 6 41 6,7 41 6, 8 41 6, 9 41 6,10 41 6,11 41 6,12 41 6,13 41 6, 14 41 6,15 41 6,16 41 6,17 41 6,18 41 6,19 41 6,20 41 6,21 41 6, 22 41 6, 23 41 6, 24 41 6, 25 41 6, 26 41 6 27 41 6, 28 41 6, 29 41 6,30 41 6, 31 41 6,32 41 6,33 41 6, 34 41 6,35 41 6,36 41 6, 37 41 6,38 41 6,39 41 6, 40 41 6,41 41 6,42 41 6,43 41 6, 44 41 6, 45 41 6,46 41 6,47 41 6, 48 41 6,49 41 6,50 41 6, 51 41 3 ,52 41 6,53 41 6, 54 41 6, 1 42 6, 2 42 6, 3 42 6, 4 42 6,5 42 6, 6 42 6, 7 42 6,8 42 6, 9 42 6,10 42 6,11 42 6, 12 42 6,13 42 6,14 42 6,15 42 6,16 42 6,17 42 6,18 42 6,19 42 6,20 42 6,21 42 6, 22 42 6, 23 42 6, 24 42 6, 25 42 6, 26 42 6, 27 42 6, 28 42 6, 29 42 6, 30 42 6,31 42 6 36 Printed from Mimosa 13 07 15 PCT/US96/20/26 32 42 6, 33 42 6,34 42 6,35 42 6,36 42 6,37 42 6, 38 42 6, 39 42 6, 40 42 6,41 42 6, 42 42 6, 43 42 6,44 42 6,45 42 6,46 42 6 47 42 6,48 42 6, 49 42 6, 50 42 6, 51 42 3 , 52 42 6, 53 42 6, 54 42 6,1 43 6, 2 43 6,3 43 6,4 43 6 5 43 6, 6 43 6,7 43 6,8 43 6,9 43 6, 10 43 6,11 43 6,12 43 6,13 43 6,14 43 6,15 43 6,16 43 6,17 43 6,18 43 6,19 43 6, 5 20 43 6, 21 43 6,22 43 6, 23 43 6,24 43 6, 25 43 6, 26 43 6, 27 43 6, 28 43 6,29 43 6, 30 43 6,31 43 6,32 43 6,33 43 6, 34 43 6, 35 43 6,36 43 6, 37 43 6,38 43 6,39 43 6, 40 43 6, 41 43 6,42 43 6,43 43 6,44 43 6,45 43 6,46 43 6, 47 43 6,48 43 6, 49 43 6, 50 43 6, 51 433 , 52 43 6,53 43 6, 54 43 6,1 44 6, 2 44 6,3 44 6,4 44 6,5 44 6, 6 44 6, 7 44 6,8 44 6,9 44 6,10 44 6,11 44 6,12 44 6,13 44 6,14 44 6,15 44 6,16 44 6,17 44 6, 10 18 44 6,19 44 6,20 44 6,21 44 6,22 44 6,23 44 6,24 44 6, 25 44 6,26 44 6,27 44 6, 28 44 6,29 44 6,30 44 6,31 44 6,32 44 6, 33 44 6,34 44 6, 35 44 6,36 44 6,37 44 6, 38 44 6/ 39 44 6,40 44 6,41 44 6,42 44 6,43 44 6,44 44 6, 45 44 6,46 44 6,47 44 6, 48 44 6,49 44 6,50 44 6,51 44 3 , 52 44 6,53 44 6 and 54 44 6 Additional exemplary compounds include the same compounds the numbered designations listed above represent, except that the base ademn-9-yl replaces cytosin-l-yl We incorporate herein by reference the series of compound designations listed in the present application that begins with 113 and ends with 54 44 6 where B is ademn-9-yl 20 One can select an NPE based on the substrate specificity of esterases and/or carboxypeptidases one expects to find within cells where ester hydrolysis is desired If the specificity of these enzymes is unknown, one determines the desired substrate specificity by screening a plurality of nucleotide phosphonate esters This will be apparent from assay either of the generation of free 25 phosphonate or of antiviral activity One selects compounds that are (l) not hydrolyzed or hydrolyzed comparatively slowly in the upper gut, (u) gut and cell permeable and (ill) hydrolyzed in the cell cytoplasm and/or systemic circulation The artisan uses screens with cells from particular tissues to identify precursors that are released in organs susceptible to a target viral infection, e g in the case of 30 liver, precursor drugs that the liver hydrolyzes One treats other infections, e g , CMV or HIV, with a precursor that the infected subject preferably hydrolyzes at substantially the same rate and to substantially the same degree in all or most tissues, with no one tissue preferentially hydrolyzing the nucleotide phosphonate esters to a significant degree 35 The assays used can be those known in the art including intestinal lumen stability, cell permeation, liver homogenate stability and plasma stability assays 37 Printed from Mimosa 13 07 15 WO 97/24361 PCTAJS96/20226 One uses these assays to determine the bioavailability characteristics of particular active nucleotide phosphonate esters according to routinely used methods The hydrolysis products of the nucleotide phosphonate esters have activity against viruses, malignant cells and/or parasitic protozoans For example, 9-(3-5 hydroxy-2-phosphonylmethoxypropyl (HPMP) and analogs of purine (adenine (A), guanine (G), 2,6-diaminopurine (DAP), 2-monoaminopunne (MAP), hypoxanthine (Hx) and pyrimidine (cytosme (C), uracil (U), thymine (T) were evaluated for antiviral properties (S)-HPMPA, (S)-cychc HPMPA, (S)-HPMPC, (S)-HPMPG and (S)-HPMPDAP were active against herpes simplex virus, type 1 10 or 2 (HSV-1 and -2) (S)-HPMPA and (S)-cyclic HPMPA were active against varicella zoster virus (VZV) (S)-HPMPC was active against human cytomegalovirus (HCMV) in both humans and arumals (S)-HPMPA and (S)-cychc HPMPA were shown to be active against adenovirus and vaccinia virus (S)-HPMPA has potent and selective activity against a broad spectrum of 15 DNA viruses, including HSV-1 and 2, VZV, thymidine kinase-deficient (TK~) mutants of herpes simplex virus, HCMV, phocid herpesvirus type 1 (seal herpesvirus, SeHV), simian herpesvirus type 1 (SHV-1), or pseudorabies virus or Aujeszky's disease virus), bovid herpesvirus type 1 (infectious bovine rhinotracheitis virus, BHV-1), equid herpesvirus type 1 (equine abortion virus, 20 EHV-1), African swine fever (ASF) virus, vaccinia virus, and human adenoviruses, and retroviruses such as murine sarcoma virus (MSV) The literature also reports that in mice and rabbits in vivo, the compound is effective against both local and systemic infections with herpes simplex virus type 1, including herpetic keratitis caused by a TK~ mutant which is resistant to the 25 classical antiherpes drugs (DeClercq, E , et al, Antiviral Res (1987) £ 261-272, DeClercq, E , et al, Nature (1986) 222 464-467, Gil-Fernandez, C , et al, Antiviral EfiS (1987) Z151-160, Baba, M , et al, Antimicrob Agents Chemother (1987) 21337-339) Researchers have also evaluated phosphonylmethoxyalkylpurine analogs 30 for their antitumor activity in murine tumor models They found that HPMPA was active against intraperitoneal P388 leukemia As indicated above, the compounds of the invention are useful for treatment of microbial infections, for treatment of tumors or for other indications described below Microbial infections include infection by viruses, 35 parasites, yeasts and fungi Exemplary viral infections that may be treated include infections mediated by DNA or RNA viruses including herpesviruses (CMV, HSV 1, HSV 2, EBV, varicella zoster virus , bovid herpesvirus type 1, 38 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 equid herpesvirus type 1), papillomaviruses (HPV types 1-55), fiaviviruses (including African swine fever virus and Japanese encephalitis virus), togaviruses (including Venezuelan equine encephalomyelitis virus), influenza viruses (types A-C), retroviruses (HIV 1, HIV 2, HTLV I, HTLV II, SIV, HBV, 5 FeLV, FTV, MoMSV), adenoviruses (types 1-8), poxviruses (vaccinia virus), enteroviruses (polio virus type 1-3, hepatitis A virus), gastroenteritis viruses (Norwalk viruses, rotaviruses), hantaviruses (Hantaan virus), papovaviruses, rhmoviruses, paramflumza virus types 1-4, rabies virus, and the like One determines the activity of individual nucleotide analogs and 10 nucleotide phosphonate esters by routine assay of antiviral (or other antimicrobial) activity using enzyme inhibition assays, tissue culture assays, animal model assays and/or other acceptable assays Researchers believe that nucleotide phosphonates such as HPMPC exert antimicrobial activity, at least m part, by a two step enzyme-mediated conversion 15 to a diphosphate, followed by incorporation of the diphosphorylated nucleotide analog into nucleic acids Virus-mediated or microbe-mediated incorporation of the diphosphates into nucleic acid uses viral or other microbial DNA or RNA polymerases (bacterial, retroviral, etc) One can use the NPEs (1) in tissue culture systems to eliminate or reduce 20 viral spread or growth during the production of biopharmaceuticals or other products (such as proteins or vaccines), (2) to eliminate or reduce viral spread or growth in clinical samples (such as blood), and (3) to reduce or block growth of viruses in tissue culture without interfering with protein production One can treat parasitic protozoan infections using the NPEs The term 25 protozoa includes those members of the subphyla Sarcomastigophora and Sporozoa of the phylum Protozoa More particularly, the term protozoa as used herein includes those genera of parasitic protozoa which are important to man because they either cause disease in man or in his domestic animals These genera are for the most part found classified in the superclass Mastighphora of 30 the subphylum Sarcomastigophora and the class Telosporea of the subphylum Sporozoa in the classification accoidmg to Baker (1969) Illustrative genera of these parasitic protozoa include Histomonas, Pneumocystis, Trypanosoma, Giardia, Trichomonas, Eimcria, Isopora, Lcishmania, Entamoeba, Toxoplasma and Plasmodium Parasitic protozoans include Plasmodium falciparum, 35 Plasmodium bcrghct, Plasmodium malariac, Plasmodium vivax, Leishmama brazihensis, Leishmama donovani, Trypanosoma cruzi, Trypanosoma brucei, Trypanosoma rhodesiense, Pneumocystis carinu, Entamoeba histolytica, 39 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 Trichomonas vaginalis and the like (de Vries, E , et al, Met] Biochem Parasitol (1991) ^7 43-50) NPEs of the invention and/or their corresponding nucleotide analogs can also be used to treat yeast or fungal infections caused by Candida glabrata, Candida tropicahs, Candida albicans, and other Candida species 5 Cryptococcus species including Cryptococcus neoformans, Blastomyces species including Blastomyces dermatidis, Torulopsis species including Torulopsis glabrata, Coccidioidcs species including Coccidioides immitis, Aspergillus species and the like Compounds of the invention and their physiologically acceptable salts 10 (hereafter collectively referred to as the active ingredients) may be administered by any route appropriate to the condition to be treated, suitable routes including oral, rectal, nasal, topical (including ocular, buccal and sublingual), vaginal and parenteral (including subcutaneous, intramuscular, intravenous, intradermal, intrathecal and epidural) The preferred route of administration may vary with 15 for example the condition of the recipient One can administer the active ingredients alone, but it is preferable to present them as pharmaceutical formulations The formulations, both for veterinary and for human use, of the present invention comprise at least one active ingredient, as above defined, together with one or more acceptable carriers 20 therefor and optionally other therapeutic ingredients The carrier(s) must be "acceptable' in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof The formulations include those suitable for oral, rectal, nasal, topical (including buccal and sublingual), vaginal or parenteral (including subcutaneous, 25 intramuscular, intravenous, intradermal, intrathecal and epidural) administration One can conveniently prepare the formulations in unit dosage form which using any of the methods known in the art of pharmacy Such methods include the step of bringing into association the active ingredient with the carrier which constitutes one or more accessory ingredients In general, one 30 prepares the formulations by uniformly and intimately bnnging into association the active ingredient with liquid carriers or finely divided solid carriers or both, and then, if necessary, shaping the product One can prepare the formulations of the present invention suitable for oral administration as discrete units such as capsules, cachets or tablets each 35 containing a predetermined amount of the active ingredient, as a powder or granules, as solution or a suspension in an aqueous liquid or a non-aqueous 40 Printed from Mimosa 13 07 15 liquid, or as an oil-in-water liquid emulsion or a water-in-oil liquid emulsion One can also prepare the active ingredient as a bolus, electuary or paste One can make a tablet by compression or molding, optionally with one or more accessory ingredients One prepares compressed tablets by compressing in a 5 suitable machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, preservative, surface active or dispersing agent One can make moulded tablets by molding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent One can optionally coat or score the tablets and formulate 10 them to provide a slow or controlled release of the active ingredient One can apply, for infections or the eye or other external tissues e g mouth and skin, the formulations as a topic al ointment or cream containing the active mgredient(s) in an amount of, for example, 0 075 to 20% w/w (including active mgredient(s) in a range between 0 1% and 20% in increments of 0 1% w/w such 15 as 0 6% w/w, 0 7% w/w, etc), preferably 0 2 to 15% w/w and most preferably 0 5 to 10% w/w One can employ an ointment formulation with the active ingredients in either a paraffmic or a water-rruscible ointment base Alternatively, one can formulate the active ingredients in a cream with an oil-in-water cream base If desired, the aqueous phase of the cream base may include, for example, 20 at least 30% w/w of a polyhydnc alcohol, i e an alcohol having two or more hydroxyl groups such as propylene glycol, butane 1,3-diol, mannitol, sorbitol, glycerol and polyethylene glycol (including PEG 400) and mixtures thereof The topical formulations may desirably include a compound which enhances absorption or penetration of the active ingredient through the skin or other 25 affected areas Examples of such dermal penetration enhancers include dimethyl sulphoxide and related analogs The oily phase of the emulsions of this invention may be constituted from known ingredients in a known manner While the phase may comprise merely an emulsifier (otherwise known as an emulgent), it desirably comprises a 30 mixture of at least one emulsifier with a fat or an oil or with both a fat and an oil Preferably, a hydrophilic emulsifier is included together with a lipophilic emulsifier which acts as a stabilizer It is also preferred to include both an oil and a fat Together, the emulsifier(s) with or without stabihzer(s) make up the so-called emulsifying wax, and the wax together with the oil and fat make up the so-35 called emulsifying ointment base which forms the oily dispersed phase of the cream formulations 41 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 Emulgents and emulsion stabilizers suitable for use in the formulation of the present invention include Tween® 60, SDan® 80, cetostearyl alcohol, benzyl alcohol, myristyl alcohol, glyceryl mono-stearate and sodium lauryl sulfate The choice of suitable oils or fats for the formulation is based on achieving 5 the desired cosmetic properties, since the solubility of the active compound in most oils likely to be used in pharmaceutical emulsion formulations is very low Thus the cream should preferably be a non-greasy, non-staining and washable product w^h suitable consistency to avoid leakage from tubes or other containers Straight or branched chain, mono- or dibasic alkyl esters such as di-10 isoadipate, isocetyl stearate, propylene glycol diester of coconut fatty acids, isopropyl myristate, decyl oleate, isopropyl palmitate, butyl stearate, 2-ethylhexyl palmitate or a blend of branched chain esters known as Crodamol CAP may be used, the last three being preferred esters These may be used alone or in combination depending on the properties required Alternatively, high melting 15 point lipids such as white soft paraffin and/or liquid paraffin or other mineral oils can be used Formulations suitable for topical administration to the eye also include eye drops wherein the active ingredient is dissolved or suspended in a suitable carrier, especially an aqueous solvent for the active ingredient The active 20 ingredient is preferably present in such formulations in a concentration of 0 5 to 20%, advantageously 0 5 to 10% particularly about 1 5% w/w Formulations suitable for topical administration in the mouth include lozenges comprising the active ingredient in a flavored basis, usually sucrose and acacia or tragacanth, pastilles comprising the active ingredient in an inert basis 25 such as gelatin and glycerin, or sucrose and acacia, and mouthwashes comprising the active ingredient in a suitable liquid carrier Formulations for rectal administration may be presented as a suppository with a suitable base comprising for example cocoa butter or a salicylate Formulations suitable for nasal administration wherein the carrier is a 30 solid include a coarse powder having a particle size for example in the range 20 to 500 microns (including particle sizes in a range between 20 and 500 microns in increments of 5 microns such as 30 microns, 35 microns, etc), which is administered in the manner in which snuff is taken, i e by rapid inhalation through the nasal passage from a container of the powder held close up to the 35 nose Suitable formulations wherein the carrier is a liquid, for administration as for example a nasal spray or as nasal drops, include aqueous or oily solutions of the active ingredient Formulations suitable for aerosol administration may be 42 Printed from Mimosa 13 07 15 prepared according to conventional methcds and may be delivered with other therapeutic agents such as pentamidine for treatment of Pneumocystis pneumonia Formulations suitable for vaginal administration may be presented as 5 pessaries, tampons, creams, gels, pastes, foams or spray formulations containing in addition to the active ingredient such carriers as are known in the art to be appropriate Formulations suitable for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, 10 bactenostats and solutes which render the formulation isotonic with the blood of the intended recipient, and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents The formulations may be presented in urut-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dned (lyophilized) condition requiring 15 only the addition of the sterile liquid carrier, for example water for injections, immediately prior to use Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described Preferred unit dosage formulations are those containing a daily dose or urut daily sub-dose, as herein above recited, or an appropriate 20 fraction thereof, of an active ingredient It should be understood that in addition to the ingredients particularly mentioned above the formulations of this invention may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavoring agents 25 The present invention further provides veterinary compositions comprising at least one active ingredient as above defined together with a veterinary carrier therefor Veterinary carriers are materials useful for the purpose of administering the composition and may be solid, liquid or gaseous materials which are 30 otherwise inert or acceptable in the veterinary art and are compatible with the active ingredient These veterinary compositions may be administered orally, parenterally or by any other desired route Compounds of the invention can be used to provide controlled release pharmaceutical formulations containing as active ingredient one or more 35 compounds of the invention ("controlled release formulations ) in which the release of the active ingredient can be controlled and regulated to allow less frequency dosing or to improve the pharmacokinetic or toxicity profile of a given 43 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 invention compound Controlled release formulations adapted for oral administration in which discrete units comprising one or more compounds of the invention can be prepared according to conventional methods Controlled release formulations may be employed for the treatment or prophylaxis of 5 various microbial infections particularly human bacterial, human parasitic protozoan or human viral infections caused by microbial species including Plasmodium, Pneumocystis, herpesviruses (CMV, HSV 1, HSV 2, VZV, and the like), retroviruses, adenoviruses and the like The controlled release formulations can be used to treat HIV infections and related conditions such as 10 tuberculosis, malaria, Pneumocystis pneumonia, CMV retinitis, AIDS, AIDS-related complex (ARC) and progressive generalized lymphadeopathy (PGL), and AIDS-related neurological conditions such as multiple sclerosis, and tropical spastic paraparesis Other human retroviral infections that may be treated with the controlled release formulations according to the invention include Human 15 T-cell Lymphotropic virus (HTLV)-I, -II and -IV, HIV-1 and HTV-2 infections The invention accordingly provides pharmaceutical foimulations for use in the treatment or prophylaxis of the above-mentioned human or vetnnary conditions and microbial infections For each of the above-indicated utilities and indications the amount 20 required of an active ingredient (as above defined) will depend upon a number of factors including the seventy of the condition to be treated and the identity of the recipient and will ultimately be at the discretion of the attendant physician or veterinarian In general however, for each of these utilities and indications, a suitable, effective dose will be in the range 0 1 to 250 mg per kilogram bodyweight 25 of recipient per dose (including active mgredient(s) in a range between 0 1 mg and 250 mg/Kg/dose in increments of 0 5 mg/Kg/dose such as 2 5 mg/Kg/dose, 3 0 mg/Kg/dose, 3 5 mg/Kg/dose, etc), preferably in the range 0 5 to 50 mg per kilogram body weight per dose and most preferably m the range 1 to 15 mg per kilogram body weight per dose, an optimum dose is about 3 0 mg per kilogram 30 body weight per dose (Unless otherwise indicated all weights of active ingredient are calculated as the parent compound of formula I for salts thereof the figures would be increased proportionately) The desired dose is preferably presented as one dose or two sub-doses administered at appropriate intervals throughout a period of one to seven days It is preferred to administer a dose 35 once every 2, 3,4, 5 or 6 days The doses may be administered m unit dosage forms The desired dose is may be presented as one, two, or three sub-doses administered at appropriate intervals throughout the one to seven day period 44 Printed from Mimosa 13 07 15 These sub-doses may be administered in unit dosage form, for example, containing 10 to 1000 mg, and or 100 to 500 mg of active ingredient per urut dosage form The formulations should be desirably administered to achieve peak piasma concentrations of the active compound of from about 1 to about 100 jiM, 5 preferably about 2 to 50 |iM, most preferably about 3 to about 30 ^iM Methods for administering esters of cHPMPC to humans is described in U S S N 08/193,341, which is incorporated herein by reference Formula (1) nucleotide phosphonate esters will generally (1) have a higher oral bioavailability than the corresponding uncychzed nucleotide analog (e g, 10 cHPMPC compared to HPMPC) and/or (2) will exibit reduced toxicity when compared with the same dose of the corresponding uncychzed nucleotide analog, and/or (3) will have greater efficacy when compared with the same dose of the corresponding uncychzed nucleotide analog The compounds of the invention may be employed in combination with 15 other therapeutic agents for the treatment or prophylaxis of the infections or conditions indicated above Examples of such further therapeutic agents include agentb that are effective for the treatment or prophylaxis of viral, parasitic or bacterial infections or assoaated conditions oi for treatment of tumors or related conditions include 3'-azido-3'-deoxythymidine (zidovudine, AZT), 2'-deoxy-3'-20 thiacytidine (3TC), 2',T-dideoxy-2',3'-didehydroadenosine (D4A), 2,3'-dideoxy-2',3'-didehydrothymidine (D4T), carbovir (carbocyclic 2',3'-dideoxy-2',3'-didehydroguanosine), 3 -azido-2,3'-dideoxyuridine, 5-fluorothymidme, (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU), 2-chlorodeoxyadenosine, 2-deoxycoformycin, 5-fluorouracil, 5-fluorouridme, 5-fluoro-2'-deoxyundine, 5-25 tnfluoromethyl-2'-deoxyuridine, 6-azauridine, 5-fluoroorotic acid, methotrexate, tnacetylundine, l-(2'-deoxy-2'-fluoro-l-(3-arabinosyl)-5-iodocytidine (FLAC), tetrahydro-iixudazo(4/5,l-]k)-(l,4)-benzodiazepin-2(lH)-thione (TIBO), 2'-nor-cyclicGMP, 6-methoxypunne arabinoside (ara-M), 6-methoxypurine arabinoside 2'-0-valerate, cytosine arabinoside (ara-C), 2',3'-dideoxynucleosides such as 2',3'-30 dideoxycytidine (ddC), 2',3'-dideoxyadenosine (ddA) and 2',3'-dideoxyinosine (ddl), acyclic nucleosides such as acyclovir, penciclovir, famciclovir, ganciclovir, HPMPC, PMEA, PMEG, PMPA, PMPDAP, FPMPA, HPMPA, HPMPDAP, (2R, 5R)-9-[tetrahydro-5-(phosphonomethoxy)-2-furanyl]ademne, (2R, 5R)-l-[tetrahydro-5-(phosphonomethoxy)-2-furanyl]thymine, other antivirals including ribavirin 35 (adenine arabinoside), 2-thio-6-azauridine, tubercidin, aurintricarboxylic acia, 3-deazaneoplanocin, neoplanocin, nmantidine, adamantine, and foscarnet (tnsodium phosphonoformate), antibacterial agents including bactericidal 45 Printed from Mimosa 13 07 15 fluoroquinolones (ciprofloxacin, pefloxacin and the like), aminoglycoside bactericidal antibiotics (streptomycin, gentamicin, amicacm and the like) p-lactamase inhibitors (cephalosporins, penicillins and the like), other antibacterials including tetracycline, isoniazid, rifampin, cefoperazone, 5 claithromycm and azithromycin, antiparasite or antifungal agents including pentamidine (l,5-bis(4'-aminophenoxy)pentane)/ 9-deazainosine, sulfamethoxazole, sulfadiazine, quinapyramme, quinine, fluconazole, ketoconazole, itraconazole. Amphotericin B, 5-fIuorocytosme, clotrimazole, hexadecylphosphocholine and nystatin, renal excretion inhibitors such as 10 proberucid, nucleoside transport inhibitors such as dipyridamole, dilazep and mtrobenzylthiomosine, immunomodulators such as FK506, cyclosporin A, thymosin a-1, cytokines including TNF and TGF-|J, interferons including IFN-a, IFNJ-[1 and IFN-y, mterleukins including interleukin I, II, III, IV, V, VI, VH, vm, X, XII, XIII macrophage/granulocyte colony stimulating factors including GM-15 CSF, G-CSF, M-CSF, cytokine antagonists including anh-TNF antibodies, anti-interleukin antibodies, soluble interleukin receptors, protein kinase C inhibitors and the like The compounds of this invention, or the biologically active substances produced from these compounds by hydrolysis in vivo, are used as immunogens 20 to prepare antibodies capable of binding specifically to the compounds or their hydrolysis products The immunogenic compositions therefore are useful as intermediates in the preparation of antibodies for use in diagnostic or quality control assays for the compounds or their hydrolysis products The antibodies are useful for measuring the presence, absence or amounts of the compounds by 25 any convenient homogenous or heterogenous procedure such as fluorescence polarization immunoassay, fluorescence immunoassay (using fluorescent labels such as fluorescein and the like), radioimmunoassay, enzyme immunoassay (using enzyme indicators such as alkaline phosphatase, horseradish peroxidase, glucose oxidase, urease and the like) and nephelometnc inhibition assay by 30 described methods (WO 92/22639, incorporated herein by reference) Such assays usually require a tracer (such as a fluorescent or radiolabeled labeled invention compound), an antibody and the sample to be analyzed containing the compound The immunogens of this invention contain the precursor or hydrolytic 35 products in association with an immunogenic substance such as a protein or peptide Immunogenic substances include adjuvants such as Freund's adjuvant, immunogenic proteins such as viral, bacterial, yeast, plant and animal 46 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 polypeptides, in particular keyhole limpet hemocyanin, serum albumin, bovine thyroglobulin or soybean trypsin inhibitor, and immunogenic polysaccharides Typicallv, the precursor or a compound having the structure of a precursor hydrolytic product is covalently conjugated to an immunogenic polypeptide or 5 polysaccharide by the use of a polyfunctional (ordinarily Afunctional) cross-linking agent Methods for the manufacture hapten immunogens are conventional per sc, and any of the methods previously used foi conjugating haptens to immunogenic polypeptides or the like are suitably employed here as well, taking into account the functional groups on the precursors or hydrolytic 10 products which are available for cross-linking Typically the polypeptide is conjugated to a site on the heterocyclic base functionality of the compound or hydrolysis product rather than to a site on the alkyl or substituted-alkyl phosphonate moiety In general, the site will be an amino group located on the purine or pyrimidine moiety of the nucleoside 15 phosphonate, at the 5 position of pynmidines (such as cytosine or uracil), at the 1 position of purines (such as adenosine or guanine) or, for compounds having a cyclic structure corresponding to a sugar or sugar analog and having a free hydroxyl group, through the hydroxyl group (usually at the 3' or 2' positions) Alternatively, the precursor compound is cross-linked through the phosphonate, 20 typically by amidation or esterification of the phosphonate by the polypeptide itself or by a cross-linking functionality covalently bonded to the polypeptide The conjugates are prepared in conventional fashion For example, N-hydroxysuccirumide, succinic anhydride or alkN=C=Nalk are useful in preparing the conjugates of this invention The conjugates contain a precursor, its 25 hydrolysis product, or both Ordinarily, the conjugates will comprise the hydrolysis product, i e , the biologically active drug The conjugates are separated from starting materials and byproducts using chromatography or the like, and then are sterile filtered and vialed for storage Animals are typically immunized against the immunogenic conjugates or 30 derivatives by combining 1 mg or 1 jag of conjugate (for rabbits or mice, respectively) with 3 volumes of Freund's complete adjuvant and injecting the solution intradermally at multiple sites One month later the animals are boosted with 1/5 to 1/10 the original amount of conjugate in Freund's complete adjuvant (or other suitable adjuvant) by subcutaneous injection at multiple sites 35 7 to 14 days later animals are bled and the serum is assayed for the desired antibody titer Animals are boosted until the titer plateaus Preferably, the animal is boosted with the conjugate in which the precursor or product is linked 47 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 to a different protein, through a different cross-linking agent or both Optionally, aggregating agents such as alum are used to enhance the immune response After immunization, monoclonal antibodies are prepared by recovering immune lymphoid cells (typically spleen cells or lymphocytes from lymph node 5 tissue) from immunized animals and immortalizing the cells in conventional fashion, e g, by fusion with myeloma cells or by Epstein-Barr virus transformation and screening for clones expressing the desired antibody The hybndoma technique described originally be Kohler and Milstein, Eur. T Immunol. (1976) 6511 has been widely applied to produce hybrid cell lines that 10 secrete high levels of monoclonal antibodies against many specific antigens It is possible to fuse cells of one species with another However, it is preferably that the source of the immunized antibody producing cells and the myeloma be from the same species The hybrid cell lines are maintained in culture in vitro The cell lines of 15 this invention are selected or maintained in a hypoxanthine-aminopterin thymidine (HAT) medium However, the established hybndoma cell line can be maintained on a variety of nutritionally adequate media The secreted antibody is recovered from culture by conventional methods such as precipitation, ion exchange ciuomatography, affinity chromatography, or the like The antibodies 20 described herein are also recovered from hybndoma cell cultures by conventional methods for purification of IgG or IgM as the case may be that heretofore have been used to purify immunoglobulins from pooled plasma, e g , ethanol or polyethylene glycol precipitation procedures The purified antibodies are sterile filtered, and optionally are conjugated to a detectable marker such as 25 an enzyme or spin label for use in diagnostic assays of test samples The antibodies of this invention are obtained from any animal species, but ordinarily are murine or rat Once a monoclonal antibody having the desired specificity and affinity is obtained, other conventional modifications of the antibodies are within the scope of this invention For example, the 30 complementarity determining regions of an animal antibody, together with as much of the framework domain as is needed, are substituted into an antibody of another animal speues or class to produce a cross-class or cross-species chimenc antibody Fragments or other amino acid sequence variants of monoclonal antibodies also are encompassed within the meaning of antibody as that term is 35 used herein, for example, Fab, Fab' or (Fab')2 fragments, single chain antibodies, bi or polyspecific antibodies, and the like 48 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/10226 The antibodies of this invention are from any suitable class or isotype, e g IgG, IgM, IgA, IgD or IgE They may or may not participate in complement binding or ADCC Typically, hybridomas which are capable of binding to the immunogen are 5 screened for the ability to bind to the hapten itself in typical test samples (plasma, serum and the like) with the requisite degree of affinity The desired affinity will depend upon the use intended for the antibody, but should be adequate to function in a conventional competitive-type ELISA or radioimmunoassays, or in conventional EMIT immunoassays 10 The antibodies of this invention are used m such assays together with a labeled from of the precursor or its hydrolytic product Alternatively, the antibody is labeled Suitable labels are well-known and include radioisotopes, enzymes, stable free radicals, fluorophors, chemiluminescent moieties and other detectable groups heretofore employed to prepare covalent conjugates for use in 15 assays Methods for linking the labels to Iigand ammo groups, or amino acid side chains or termini ot polypeptides, are known and are suitable for use herein Other suitable linking methods will be apparent to the ordinary artisan The antibodies and labeled ligands herein optionally are assembled into kits for use in therapeutic drug monitoring or evaluation, or for process quality 20 control, and used the conventional manner cHPMPC and the cyclic analogs ot other cHPMPs are prepared by a number of methods from the free hydroxy phosphoruc acid These methods include treatment with DCC in DMF, reaction with Vilsmeier's reagent (C1CH=N(CH3)2C1), or methods of phosphate activation known per se In one 25 method for the preparation of a cHPMP from the corresponding phosphonate nucleotide analog, the phosphonate is (a) treated with C1CH=N(CH3)2CI to yield the phosphonylchloridate and (b) optionally the phosphonylchoridate is reacted with a nucleophile (preferably at low temperature, e g lower than about -20*C) such as an alcohol or amine to produce one of the intermediates described above 30 In a further step the product of steps (a) or (b) are subject to hydrolysis or protonolysis (typically acid protonolysis) respectively to yield the cHPMP (treatment of the product of step (a)) or its intermediate (treatment of the product of step (b)) Vilsmeier's reagent is advantageously produced in situ by combining SOCI2, PCI5, POCI3, COCI2 or the like with DMF Advantageously, the product of 35 step (a) is not purified or separated from the reaction mixture before being reacted with the nucleophile, a distinct economic advantage for this synthetic route The 49 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US9G/20226 compounds of structure (la) and (Va) are readily made from their uncycltzed counterparts by the same methods, e g treatment with DCC in DMF Substituted and unsubstituted alkyl, aryl, heteroaryl, arylalkyl and heteroarylalkyl esters of cHPMPs typically are made by reacting the appropriate 5 HPMP compound with SOCI2/DMF to yield the activated phosphonylchloride (see Scheme 1), followed by treatment with the corresponding nucleophile (e g alkoxide, phenolate, amine, etc) to yield the protected intermediate formamidme which is subsequently hydrolyzed to the target compound Alternatively, esters can also be prepared as depicted in Scheme 2 The N-,0- protected intermediate 10 phosphonate diester is obtained from the three building blocks by known methods The N- and O- protecting groups are subsequently removed followed by treatment of the phosphonate diester J with NaH leading to cyclization yielding target compound 4 A third method for the synthesis of cHPMP esters entails alkylation of the cHPMP using common alkylating agents R15L (where L 15 is a leaving group) such as alkyl hahdes, tosylates, diazoalkanes and the like (see Scheme 3) This method is particularly useful for preparing acyloxyalkyl esters by treatment of the cHPMP with the corresponding acyloxyalkylhahde In an exemplary method for the preparation of acyloxyalkyl esters of cHPMPs, as shown in more detail in Example 12, DCC and R16C(0)0CH2C1 are 20 reacted with the cyclic compound, but in contradistinction with prior methods the stoichiometric proportion of DCC R16C(0)0CH2C1, cyclic HPMP is 1-2 1-2 1 Use of such low proportions of reactants lessens side reactions with any exocychc amino group of B and thereby greatly improves yields Rlfi is H or is C3-C12 alkyl which is unsubstituted or substituted by substituents independently selected 25 from the group consisting of OH, O, N and halogen, Cj-Cft aryl which is unsubstituted or substituted by substituents independently selected from the group consisting of OH, O, N and halogen or C3-C9 aryl-alkyl which is unsubstituted or substituted by substituents independently selected from the group consisting of OH, O, N and halogen 30 Each of the following schemes exemplify HPMPC as the nucleotide analog However, any B is employed in place of cytosine, provided that any exocychc oxo or amino groups are protected as required Also, step 3 of scheme 1 will be omitted when B contains no exocychc amine 50 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 nh2 J. n O^N XJ O V 'o' "oh 1) DCC or H 2) (a) Vilsmeier's Reagent / (b) Hydrolysis N"^N\ nh, n^fi -•s ° V „ O^^N O 1) Vilsmeier's . *^1 ^O il^OH n U ^O ,P"°H Reagent OH OH O CI 2) Nucleopnile (Nu) nh, JL n O^N n "jj Jj 3) Protonolysis N h J- r N N I 1. P"° k^Os, °' SNu i "°'\u Scheme 1 51 Printed from Mimosa 13 07 15 NH Bz N O^^N" I H <1 OTr t NH N^ N Bz ° « II ,OR15 °\/p\ OR "OTr NH2 -A- .

O N X) NaH/DMF O O + TsO^^,P(OR15)2 NH, NH4OH/EtOH N O' O II ,OR15 V OR15 "OTr 80% AcOH NH2 X N" X. - O N XJ ° II .OR CX ^P, \ 4 ^O" ^OR15 OR OH 3 Scheme 2 52 Printed from Mimosa 13 07 15 nh2 nh2 O' Oy XOH XOR15 Scheme 3 A third method for the synthesis of cyclic HPMP esters entails alkylation of the cyclic HPMP ester as shown in Scheme 3 using common alkylating agents 5 R15Lv such as alkyl halidcs, tosylates, diazoalkanes and the like This method is particularly useful for preparing acyloxyalkyl esters by treatment of the cydic HPMP (cHPMP) with the corresponding acyloxyalkylhahde R15 is defined as the following groups and species H, alkyl (e g, C1-20 or C4-12) which is unsubstituted or substituted by substituents independently 10 selected from the group consisting of OH, O, N and halogen (F, CI, Br, I), aryl (e g , C4-12 or C3 20) which is unsubstituted or substituted by substituents independently selected from the group consisting of alkyl (e g , Ci-Cs or Q g), alkoxy (e g, Cj-Cfl or Ci^), haloalkyl (e g, Ci-Cg or C1-6,1 to 3 halogen atoms), cyano, mtro, OH, O, N and halogen, or R15 is C4-20 aryi-alkyl which is 15 unsubstituted or substituted in the aryl moiety by substituents independently selected from the group consisting of Ci-6 alkyl, Cj-g alkoxy, Ci-6 haloalkyl (1 to 3 halogen atoms), cyano, mtro, OH, O, N and halogen, or R15 is C3.24 1-acyloxy-l-alkyl (e g , C].» alkyl), or R15 is Cfi 24 1-acyloxy-l-aryl-l-alkyl (e g , Ci-6 aryl, C1.4 alkyl), or R15 is C3-24 l-acyloxy-2-alkoxy-l-alkyl (Ci b alkyl), or R15 is 20 C3-24 l-acyloxy-2-haloalkyl (Ci-» haloalkyl, 1 to 3 halogen atoms), or R15 is phenyl, 2- or 3-pyrrolyl, 2- or 3-thienyl, 2- or 4-imidazolyl, 2-, 4- or 5-oxazolyl, 3- or 4-lsoxazolyl, 2-, 4- or 5-thiazolyl, 3-, 4- or 5-isothiazolyl, 3- or 4-pyrazolyl, 2-, 3- or 4-pyndinyl, 2-, 4- or 5-pyrimidinyl, 2-, 3- or 4-alkoxyphenyl (C1-C12 alkyl including 2-, 3- and 4-methoxyphenyl or 2-, 3- or 4-ethoxyphenyl), 2-, 3- or 4-halophenyl 25 (including 2-, 3- and 4-fluorophpnyl), 2,3-, 2,4-, 2,5-, 2,6-, 3,4- or 3,5-dihalophenyl (including 2,4-difluorophenyl and 2,4-dichlorophenyl), 2-, 3- or 4-haloalkylphenyl (1 to b halogen atoms, Ci 12 alkyl including 2-, 3- and 4- 53 Printed from Mimosa 13 07 15 PCTYUS96/20226 trifluoromethylphenyl and 2-, 3- and 4-trichioromethylphenyl), 2-, 3- or 4-cyanophenyl, alkoxycarbo phenyl (C1-12 alkyl including 2-, 3- and 4-n-butoxycarbophenyl, -C6H4-C(0)-0C4H9, and including 2-, 3- or 4-hexoxycarbophenyl, -C^Hj-QOJ-OCfcHis, -C^H4-C(0)-C>C6H]]) or 2,3-, 2,4-, 2,5-, 2,6-5 , 3,4- or 3,5-diethoxycarbophenyl, 1-, 2-, 3-, or 4-pyridmyl (-C5H4N), 2-, 3- or 4-rutrophenyl, 2-, 3- or 4-haloalkylbenzyl (1 to 5 halogen atoms, C1-C12 alkyl including 4-trifluoromethylbenzyl), alkylsalicylphenyl (C1-4, C3.10 or C6-12 alkyl including 2-, 3- and 4-butylsalicyiphenyl), 2-,3- or 4-acetylphenyl, 1,8-dihydroxy-naphthyl (-O-C10H6-OH or -O-C10H6-O-), 2,2'-dihydroxybiphenyl (-O-C6H4-10 C6H4-O-, both oxygen atoms are linked to the phosphorus atom), alkoxy ethyl (Ci-6 alkyl including -CH2-CH2-O-CH3 (methoxy ethyl) and phenoxymethyl), aryloxy ethyl (C5-10 or C&-9 aryl (including phenoxy ethyl) or C5-10 or C^.y aryl substituted by OH, NH2, halo, C1-4 alkyl or C1-4 alkyl substituted by OH or by 1 to 3 halo atoms], -C6H4-CH2-N(CH3)2, N-ethylmorpholino pivaloyloxymethyl (-CH2-0-C(0)-C(CH3)3), pivaloyloxy(methoxymethyl)-methyl 20 (-CH(CH20CH3)-0-C(0)-C(CH3)3), pivaloyloxyisobutyl (-CH(CH(CH3)2)-0-C(0)-C(CH3)3) isobutyryloxy methyl (-CH2-0-C(0)-CH2-CH(CH3)2), cyclohexanoyl oxymethyl (-CH2-0-C(0)-C6Hn), phenyl (-C6H5), benzyl (-CH2-C6H5), isopropyl, t-butyl, -CH2-CH3, -(CH2)2-CH3, -(CH2)3-CH3, -(CH2)4-CH3, -(CH2)5"CH3, -CH2-CH2F, -CH2-CH2C1, -CH2-CF3, -CH2-CCI3, RA, NHRB or N(RB)2 wherein RA is 25 CH2C(0)N(RB)2, CH2C(0)0RB, CH20C(0)RB/ CH(Rb)0C(0)Rb, CH2C(RB)2CH20H, CH2ORB, NH-CH2-C(0)0-CH2CH3, N(CH3)-CH2-C(0)0-CH2CH3, CH2-0-C(0)-C6H5, CH2-O-C(O)-C10Hi5, -CH2-0-C(0)-CH2CH3 , CH2-O-C(0)-CH(CH3)2, CH2-0-C(0)-C(CH3)3, CH2-0-C(0)-CH2-C6H5, wherein RB is C1-20 alkyl which is unsubstituted or substituted by substituents independently selected 30 from the group consisting of OH, O, N and halogen (1 to 5 halogen atoms), C^-20 aryl which is unsubstituted or substituted by substituents independently selected from the group consisting of OH, O, N and halogen (1 to 5 halogen atoms) or C7- ( ( ^ / , -(CH2)2-N[(CH2)2(CH2)2]0), adamantoyl oxymethyl, pivaloyloxy(methoxyethyl)methyl (-CH(CH2CH20CH3)-0-C(0)-C(CH3)3), , -O-CH2-O-C(O)-CI0HI5), 54 Printed from Mimosa 13 07 15 aryl-alkyl which is unsubstituted or substituted by substituents independently selected from the group consisting of OH, O, N and halogen (1 to 5 halogen atoms), provided that for compounds of formulas N(RB)2, CH2C(0)N(RB)2, CH2C(0)0RB, CH20C(0)Rb, CH(RbPC(0)Rb and CH2C(RB)2CH20H, the total 5 number of carbon atoms present is less than 25 (usually the number of carbon atoms present is about 4 to about 14), or R15 is 2,3-dihydro-6-hydroxyindene, sesamol, catechol monoester, -CH2-C(0)-N(RC)2 (wherein each Rc is the same or different and Rc is H or Cj-4 or C2-10 alkyl) -CH2-S(0)(Rc), -CH2-5(0)2(Rc), -CH2-CH(0C(0)CH2Rc), -CH2(0C(0)CH2Rc), cholesteryl, a 5 or 6 carbon 10 monosaccharide, disacchande or oligosacchande (3 to 9 monosaccharide residues), enolpyruvate (H00C-C(=CH2)0), glycerol, a-D-P-diglycendes (wherein the fatty acids composing glyceride lipids generally are naturally occurring saturated or unsaturated C(,-2e, C^-ia or C&.10 fatty acids such as hnoleic, launc, myristic, palmitic, stearic, oleic, palmitoleic, linolenic and the like fatty acids), 15 tnmethoxybenzyl, triethoxybenzyl, 2-alkyl pyndmyl (C1-4 alkyl), C3-C6 aryl (including phenyl, 2- and 3-pyrrolyl, 2- and 3-thienyl, 2- and 4-irrudazolyl, 2-, 4- and 5-oxazolyl, 3- and 4-isoxazolyl, 2-, 4- and 5-thiazolyl, 3-, 4-and 5-isothiazolyl, 3- and 4-pyrazolyl, 2-, 3- and 4-pyndinyl and 2-, 4- and 5-20 pynmidmyl) substituted by 3, 4 or 5 halogen atoms or 1 or 2 atoms or groups selected from halogen, Ci-12 alkoxy (including methoxy, ethoxy, propyloxy, butyloxy, 2,3-, 2,4-, 2,5-, 2,6-, 3,4- and 3,5-dimethoxy and 2,3-, 2,4-, 2,5-, 2,6-, 3,4- and 3,5-diethoxy substituted phenyl), cyano, mtro, OH, Ci-12 haloalkyl (1 to 6 halogen atoms), C1.12 alkyl (including methyl and ethyl), C2-12 alkenyl or C2-12 alkynyl, or 25 R15 is C1-4 alkylene-C3-C^ aryl (including benzyl, -CH2CH2-C6H5, -CH2-pyrrolyl, -CH2-thienyl, -CH2-imidazolyl, -CH2-oxazolyl, -CH2-isoxazolyl, -CH2-thiazolyl, -CH2-isothiazolyl, -CH2-pyrazolyl, -CH2-pyndinyl and -CHrpyrimidinyl) substituted in the aryl moiety by 3 to 5 halogen atoms or 1 to 2 atoms or groups selected from halogen, C1.12 alkoxy (including methoxy and ethoxy), cyano, mtro, 30 OH, Cj-12 haloalkyl (1 to 6 halogen atoms, including -CH2-CCI3), Ci_j2 alkyl (including methyl and ethyl), C2-12 alkenyl or C2-12 alkynyl Methods for linking cholesteryl, saccharide and other moieties to reactive groups have been described (Hadfield Adv Pharmacol Chemother (1984) 2Q 21, Gouyette Tet Lett (1989) 2Q 6019, Ksander T Med Chem (1994) 3Z 1823) CH2C(0)N 55 Printed from Mimosa 13 07 15 Other compounds are used as intermediates m the synthesis of mixed amidate-ester nucleotide analog amidates, or in some cases, as drugs per se Other ester compounds have the formulas (R150)2P(0)-Z1-B where Z1 is defined to mean the substructure in the following representative structures, (R,sO)2-P(0)-CH2-0-CH2-CH2-B, (Rl50)2-P(0)-CH2-0-C#H(CH20H)-CH2-B, (Rl50)2-P(0)-CH2-0-C#H(CH3)-CH2-B, (R150)2-P(0)-CH2-0-C#H(CH2F)-CH2.B/ (R150)2-P(0)-CH2-0-C#H(CH=CH2)-CH2-B, (R150)2-P(0)-CH2-0-C#H(CH2N3)-CH2-B and rV"B o=p^ ^ 5s ° R,sO Vv here C# and # includes linked substituents in the (R), (S) or (RS) configurations and where each R15 is the same or different and each R15 is independently chosen Table 2 lists a group of exemplary bis esters of compounds having the structure (0R15)2P(0)-Z-B TABLE 2 ORlS» 1 -O-C6H4F 2 -o-C6H3F2 3 -O-C6H4-OCH3 4 -oc6h3-(och3)2 -o-C6H4-OC2H5 6 -0-cfh3-(0c2h5)2 7 -o-CH2-C6H4F 8 -OC6H4-(C(0)-0-C4H9)2 =p(0)-z-b** 1 =p(0)-ch2-0-ch2-ch2-b 2 =p(0)-CH20C#h(CH2-oh)-CH2-b 3 =p(0)-ch2-0-c#h(ch3k:h2-b 4 =p(0)-ch2-0-c#h(ch2f)-ch2-b =p(0)-ch2-0-cfrh(ch=ch2)-ch2-b 6 =p(0)-CH2-0-c#h(CH2n3)-CH2-b 56 Printed from Mimosa 13 07 15 H r 0=P\ o B O 9 -0-CftH4-C(0)-0-C2H5 8 R150 -0-CH2-0-C(CH3)3 11 -0-C6H5-O-C(0)0CH2CH(CH2CH3)CH2CH2CH2CH3 12 -0-C6H5-0C(0)0CH2CH2CH2CH2CH2CH2CH2CH3 5 13 OC6H5-0C(0)0CH2CH2CH2CH2CH2CH3 14 -O-C6H5-O-C(0)0CH2CH2CH2CH2CH3 -0-C6H5-0-C(0)0CH2CH2CH2CH3 16 -0-C6H5-O-C(0)0CH2CH2C6H5 1 aderun-9-yl 2 guanin-9-yl 3 cytosin-l-yl 4 2, 6-diaminopurin-9-yl 15 5 2-aminopurin-9-yl 6 5-methylcytosin-l-yl 7 5-fluorocytosin-l-vI * Monosubstituted phenyl and benzyl compounds (l e, R15 numbers 1,3, 5, etc) include 2-, 3- and 4-substituted compounds and disubstituted phenyl compounds (i e , R*5 numbers 2, 4, 6, etc) include 23-/ 2,4-, 2,5-, 2,6-, 3,4- and 3,5-substituted compounds The structure =P(0)- indicates that two bonds are occupied by OR15 *** Structures 7 and 8 have a single OR15 group linked as shown instead of two linked OR15 groups Compounds listed in Table 2 are designated herein by numbers assigned to (OR15)2 (where each R15 is the same), Z and B according to the following convention, R15 Z B The compound designations follows the scheme used in Table 1 Exemplary compounds include 111, 211, 311,411, 511,611,711, 8 1 1, 9 11,10 1 1,1111,12 1 1,13 1 1,14 11,15 11,16 11,1 2 1,2 2 1,3 2 1,4 2 1, 5 2 1, 6 2 1, 7 2 1, 8 2 1,9 2 1,10 2 1,11 21,12 2 1,13 2 1,14 2 1,15 2 1,16 2 1,1 3 1, 2 3 1,3 3 1, 4 3 1,5 3 1, 6 3 1, 7 3 1, 8 3 1,9 3 1,10 3 1,11 3 1,12 3 1,13 3 1,14 3 1, B 57 Printed from Mimosa 13 07 15 3 1, 16 3 1,1 4 1, 2 4 1,3 4 1, 4 4 1, 5 4 1, 6 4 1, 7 4 1,8 4 1, 9 4 1,10 4 1,11 4 1, 12 4 1, 13 4 1, 14 4 1,15 4 1,16 4 1,1 5 1, 2 5 1,3 5 1, 4 5 1, 5 5 1, 6 5 1, 7 5 1,8 5 1, 9 5 1, 10 5 1, 11 5 1, 12 5 1,13 5 1,14 5 1,15 5 1,16 5 1,1 6 1,2 6 1, 3 6 1, 4 6 1,5 6 1, 6 6 1, 7 6 1, 8 6 1,9 6 1,10 6 1,11 6 1,12 6 1,13 6 1,14 6 1,15 6 1, 16 6 1,1 7 1, 2 7 1, 3 7 1, 4 7 1, 5 7 1,6 7 1,771,8 7 1,9 71,10 71,11 7 1,12 71,13 71,14 7 1,15 71,16 71,1 81, 2 8 1,3 8 1,4 8 1, 5 8 1, 6 8 1, 7 8 1, 8 8 1, 9 8 1,10 8 1, 11 8 1,12 8 1,13 8 1, 14 8 1, 15 8 1,16 8 1,1 1 2, 2 1 2,3 1 2,4 1 2, 5 1 2, 6 1 2, 7 1 2,8 1 2, 9 1 2,10 1 2,11 1 2, 12 1 2, 13 1 2,14 1 2,15 1 2,16 1 2,1 2 2,2 2 2, 3 2 2, 4 2 2,5 2 2,6 2 2,7 2 2,8 2 2,9 2 2,10 2 2, 11 2 2,12 2 2,13 2 2,14 2 2,15 2 2,16 2 2,1 3 2,2 3 2,3 3 2, 4 3 2, 5 3 2, 6 3 2, 7 3 2, 8 3 2,9 3 2,10 3 2,11 3 2,12 3 2,13 3 2,14 3 2,15 3 2,16 3 2,1 4 2, 2 4 2, 3 4 2, 4 4 2, 5 4 2,6 4 2, 7 4 2, 8 4 2,9 4 2,10 4 2,11 4 2,12 4 2,13 4 2,14 4 2,15 4 2,16 4 2,1 5 2, ? 5 2,3 5 2, 4 5 2, 5 5 2, 6 5 2, 7 5 2,8 5 2,9 5 2,10 5 2,11 5 2,12 5 2,13 5 2, 14 5 2, 15 5 2,16 5 2,1 6 2, 2 6 2,3 6 2,4 6 2, 5 6 2, 6 6 2,7 6 2,8 6 2,9 6 2,10 6 2,11 6 2,12 6 2, 13 6 2,14 6 2,15 6 ?, 16 6 2,1 7 2,2 7 2,3 7 2, 4 7 2, 5 7 2, 6 7 2, 7 7 2, 8 7 2, 9 7 2,10 7 2, 11 7 2,12 7 2,13 7 2,14 7 2,15 7 2,16 7 2,1 8 2,2 8 2,3 8 2,4 8 2, 5 8 2, 6 8 2,7 8 2, 8 8 2,9 8 2,10 8 2,11 8 2,12 8 2,13 8 2,14 8 2,15 8 2,16 8 2,1 1 3, 2 1 3, 3 1 3, 4 1 3, 5 1 3, 6 1 3, 7 1 3, 8 1 3,9 1 3,10 13,111 3,12 1 3,13 1 3,14 1 3,15 1 3,16 1 3,1 2 3, 2 2 3,3 23, 4 2 3, 5 2 3, 6 2 3, 7 2 3,8 2 3, 9 2 3,10 2 3, 11 23,12 2 3,13 2 3, 14 2 3, 15 2 3,16 2 3,1 3 3, 2 3 3,3 3 3,4 3 3,5 3.3, 6 3 3, 7 3 3,8 3 3,9 3 3,10 3 3,11 3 3, 12 3 3, 13 3 3,14 3 3,15 3 3,16 3 3,1 4 3,2 4 3,3 4 3,4 4 3, 5 4 3, 6 4 3, 7 4 3,8 4 3, 9 4 3, 10 4 3, II 4 3,12 4 3( 13 4 3,14 4 3,15 4 3,16 4 3,1 5 3, 2 5 3, 3 5 3, 4 5 3, 5 5 3,6 5 3, 7 5 3, 8 5 3,9 5 3,10 53,11 5 3,12 5 3,13 5 3,14 5 3,15 5 3,16 5 3,1 6 3, 2 6 3, 3 6 3,4 6 3, 5 6 3, 6 6 3, 7 6 3, 8 6 3, 9 6 3,10 6 3,11 6 3,12 6 3,13 6 3,14 6 3,15 6 3,16 6 3,1 73, 2 73,373,473,573,673,773,873,973,1073, 1173,1273,1373,1473, 7 3,16 7 3,1 8 3, 2 8 3,3 8 3,4 8 3, 5 8 3, 6 8 3, 7 8 3,8 8 3,9 8 3,10 8 3,11 8 3,12 8 3, 13 8 3,14 8 3,15 8 3,16 8 3,1 1 4,2 1 4,3 1 4,4 1 4,5 14, 6 1 4, 7 1 4,8 1 4,9 1 4,10 14, III 4,12 1 4,13 1 4,14 1 4,15 1 4,16 1 4,1 2 4, 2 2 4, 3 2 4,4 2 4, 5 2 4, 6 2 4, 7 2 4, 8 2 4,9 2 4,10 2 4,11 2 4,12 2 4,13 2 4,14 2 4,15 2 4, 16 2 4,1 3 4, 2 3 4, 3 3 4,4 3 4, 5 3 4, 6 3 4, 7 3 4, 8 3 4,9 3 4,10 3 4,11 3 4,12 3 4,13 3 4,14 3 4,15 3 4,16 3 4,1 4 4, 2 4 4,3 4 4, 4 4 4, 5 4 4, 6 4 4, 7 4 4, 8 4 4, 9 4 4,10 4 4,11 4 4,12 4 4,13 4 4,14 4 4, 4 4,1644,154,254,354,454,554,654,754,854,954,1054,1154,1254, 13 5 4,14 5 4,15 5 4,16 5 4,1 6 4,2 6 4, 3 6 4, 4 6 4, 5 6 4,6 6 4,7 6 4,8 6 4,9 6 4,10 6 4, 11 6 4,12 6 4,13 6 4,14 6 4,15 6 4,16 6 4,1 7 4, 2 7 4, 3 7 4,4 7 4,5 7 4, 6 7 4, 7 7 4, 8 7 4,9 7 4,10 7 4,11 7 4,12 7 4,13 7 4,14 7 4,15 7 4,16 7 4,1 8 4, 2 8 4,3 8 4,4 8 4, 5 8 4, 6 8 4, 7 8 4, 8 8 4,9 8 4,10 8 4,11 8 4,12 8 4,13 8 4,14 8 4,15 8 4,16 8 4,1 1 5, 2 1 5,3 1 5,4 1 5,5 1 5,6 1 5,71 5,8 1 5,9 15,101 5,111 5,12 15,13 1 5 14 1 5, 15 1 5, 16 1 5,1 2 5, 2 2 5,3 2 5,4 2 5, 5 2 5, 6 2 5,7 2 5, 8 2 5,9 2 5,10 2 5,11 2 5, 12 2 5, 58 Printed from Mimosa 13 07 15 PCIYUS96tt0226 13 2 5,14 2 5,15 2 5,16 2 5,1 3 5,2 3 5, 3 3 5( 4 3 5,5 3 5, 6 3 5, 7 3 5, 8 3 5, 9 3 5,10 3 5, 11 3 5,12 3 5,13 3 5,14 3 5,15 3 5,16 3 5,1 4 5, 2 4 5,3 4 5, 4 4 5, 5 4 5, 6 4 5, 7 4 5, 8 4 5,9 4 5,10 4 5,11 4 5,12 4 5,13 4 5,14 4 5,15 4 5,16 4 5, 1 5 5, 2 5 5,3 5 5,4 5 5, 5 5 5, 6 5 5, 7 5 5, 8 5 5, 9 5 5, 10 5 5,11 5 5,12 5 5, 13 5 5,14 5 5,15 5 5,16 5 5,1 6 5, 5 2 6 5,3 6 5,4 6 5, 5 6 5, 6 6 5, 7 6 5, 8 6 5,9 6 5,10 6 5,11 6 5, 12 6 5,13 6 5, 14 6 5, 6 5, 16 6 5,1 7 5, 2 7 5, 3 7 5,4 7 5, 5 7 5, 6 7 5,7 7 5, 8 7 5, 9 7 5,10 7 5,11 7 5,12 7 5, 13 7 5,14 7 5,15 7 5, 16 7 5,1 8 5,2 8 5,3 8 5,4 8 5,5 8 5,6 8 5, 7 8 5, 8 8 5,9 8 5,10 8 5, 11 8 5,12 8 5,13 8 5,14 8 5,15 8 5,16 8 5,1 1 6,2 1 6,3 1 6,4 1 6,5 1 6,6 1 6,7 1 6, 8 1 6,9 1 6,10 1 6,11 1 6,12 1 6,13 1 6,14 1 6,15 1 6,16 1 6,1 2 6, 2 2 6, 3 2 6,4 2 6, 10 5 2 6, 6 2 6,7 2 6,8 2 6,9 2 6,10 2 6,11 2 6,12 2 6,13 2 6,14 2 6,15 2 6,16 2 6,1 3 6, 2 3 6,3 3 6,4 3 6,5 i 6, 6 3 6, 7 3 6, 8 3 6,9 3 6,10 3 6,11 3 6, 12 3 6,13 3 6, 14 3 6, 15 3 6,16 3 6,1 4 6, 2 4 6, 3 4 6,4 4 6,5 4 6,6 4 6,7 4 6,8 4 6, 9 4 6,10 4 6,11 4 6, ] 2 4 6, 13 4 6,14 4 6,15 4 6,16 4 6,1 5 6, 2 5 6, 3 5 6, 4 5 6,5 5 6, 6 5 6, 7 5 6, 8 5 6, 9 5 6,10 5 6, 11 5 6,12 5 6,13 5 6, 14 5 6,15 5 6,16 5 6, 1 6 6,2 6 6, 3 6 6, 4 6 6,5 6 6, 6 6 6, 7 6 6, 15 8 6 6,9 6 6,10 6 6,11 6 6,12 6 6,13 6 6,14 6 6,15 6 6,16 6 6,1 7 6, 2 7 6,3 7 6, 4 7 6, 5 7 6, 6 7 6,7 7 6,8 7 6,9 7 6,10 7 6,11 7 6, 12 7 6, 13 7 6,14 7 6,15 7 6,16 7 6,1 8 6, 2 8 6,3 8 6,4 8 6, 5 8 6,6 8 6,7 8 6, 8 8 6,9 8 6,10 8 6,11 8 6,12 8 6,13 8 6,14 8 6, 15 8 6,16 8 6,1 1 7, 2 1 7, 3 1 7,4 1 7, 5 1 7, 6 1 7, 7 1 7, 8 1 7, 9 1 7,10 1 7,11 1 7,12 1 7, 13 1 7,14 1 7,15 1 7,16 1 7,1 2 7,2 2 7, 3 2 7,4 2 7,5 2 7, 6 21, 7 2 7, 8 2 7, 9 2 7,10 2 7, 20 11 2 7,12 2 7,13 2 7,14 2 7,15 2 7,16 2 7, 1 3 7, 2 3 7,3 3 7,4 3 7,5 3 7, 6 3 7, 7 3 7, 8 3 7, 9 3 7,10 3 7,11 3 7,12 3 7,13 3 7,14 3 7,15 3 7,16 3 7,1 4 7, 2 4 7,3 4 7,4 4 7, 5 4 7, 6 4 7, 7 4 7,8 4 7, 9 4 7,10 4 7,11 4 7,12 4 7,13 4 7,14 4 7,15 4 7,16 4 7,1 5 7, 2 5 7,3 5 7,4 5 7, 5 5 7, 6 5 7, 7 5 7, 8 5 7,9 5 7( 10 5 7,11 5 7,12 5 7,13 5 7,14 5 7, 15 5 7,16 5 7,1 6 7, ? 6 7,3 6 7,4 6 7,5 6 7,6 6 7,7 6 7,8 6 7,9 6 7,10 6 7,11 6 7.12 6 7, 25 13 6 7,14 6 7,15 6 7,16 6 7,1 7 7, 2 7 7, 3 7 7,4 7 7, 5 7 7, 6 7 7, 7 7 7, 8 7 7, 9 7 7,10 7 7, 11 7 7,12 7 7,13 7 7,14 7 7,15 7 7,16 7 7,1 8 7, 2 8 7,3 8 7,4 8 7,5 8 7, 6 8 7,7 8 7, 8 8 7,9 8 7,10 8 7,11 8 7,12 8 7,13 8 7,14 8 7,15 8 7 and 16 8 7 One determines the suitability of the presence or absence of any particular 30 R15 group by stability and/or bioavailability assays (e g , stability assay in aqueous conditions such as low pH/intestinal lumen conditions or assay in the presence of cellular extracts containing esterases or by bioavailability assay using arumal models) known in the art The skilled artisan routinely performs these assays The present invention includes NPEs that comprise a protected 35 heterocyclic base These compounds are useful as synthetic intermediates and/or, as therapeutic agents per se Protected heterocyclic base compounds 59 Printed from Mimosa 13 07 15 structures, their isomers, tautomers and the salts of such compounds having the formula (5) is ^ Bl R a^i J f ° (3» where B1 is a protected heterocyclic base The exemplary reaction schemes used to synthesize protected heterocyclic base compounds shown below utilize cHPMPC as an example Analogous reactions will generate compounds composing other bases such as adenine Phosphonate alkyl and aryl esters of compounds comprising Bl are prepared, using HPMPC and cHPMPC as an example, according to the following procedures NH2 NH2 NHC(0)R17 NH2 NHC(0)R1 1) TMSC1/DMF 2) R17COCl/Pyridine 3)H30+ OH OH wherein R17 is Cj-io alkyl Either procedure is readily adapted to synthesizing 15 compounds containing protected heterocyclic bases other than cytosine, e g , adenine, guanine, 2,6-diairunopurine or 2-armnopurine Exemplary R15 and/or R17, which can be the same or different, include phenyl, substituted phenyl, -C10H15 (where C10H15 is adamantoyl), -C^-C^H^, -C^Hs, -C(CH3)3, CH(CH3)2, -CH2CH3, methyl, ethyl, butyl, t-butyl, heptanyl, nonanyl, undecanyl, lauryl, 20 steryl, undecenyl and the like The amide linkage is conveniently formed by 60 Printed from Mimosa 13 07 15 reaction of the acyl chloride with the exocychc amine linked to the base When R15 is linked to the free phosphonate the resulting ester will comprise a single isomer or a racemic mixture at the phosphorus atom Low temperature reaction conditions (lower than about -20*, e g , about -20* to about -40'C or about -40* to 5 about -80*C) tend to favor single isomer products, while reaction at higher temperatures ( above about -20*, e g -20" to 40*C) generally results in a racemic mix When a racemic mixture is obtained, the isomers can be conveniently separated by, for example, HPLC, although the mixture can be used, for example, as a synthetic intermediate or as an active antimicrobial agent, without 10 resolution Synthesis of the phenyl ester of cHPMPC at -78*C by reaction of the chlondate and phenoxide yielded a racemic mixture consisting of about £ 90% of the product as one isomer (isomer #1) at the phosphorus atom while the remaining - < 10% was present as the other isomer (isomer #2) The racemic mixture was converted to isomer #2 (> 90%) by incubation at room temperature 15 for about 10 minutes (about 10 to 30 minutes is generally suitable) with a catalytic amount of sodium phenoxide in DMF This method can be used to convert one isomer of cHPMP-B or cFIPMP-B1 (such as cHPMPC or cHPMPA) aryloxy or alkoxy ester to the other isomer with catalytic amounts of th. corresponding aryloxide ion or alkoxide ion, typically yielding about 90% of isomer Thus, one 20 can easily prepare mixtures of isomers containing 55% to 90% of one isomer The cHPMPC pivaloyloxymethyl ester synthesis yields a racemic mixture at the phosphorus atom The mixture was separated by HPLC into the two isomers which were then exposed to an rat intestinal homogenate or to a rat intestinal wash One of the isomers was converted to cHPMPC after incubation 25 in the homogenate while the other isomer was converted to HPMPC pivaloyloxymethyl monoester Both isomers were converted to HPMPC pivaloyloxymethyl monoester after incubation in the intestinal wash These results suggested that (1) in at least some cases, enzyme activity can have a differential effect on the metabolic fate of a cHPMPC ester depending on which 30 phosphorus isomer is present and (2) chemical activity (i e , the acidity of the intestinal wash) can affect the metabolic fate of a given compound in a manner that differs from enzyme activity A method to obtain heterocyclic bases comprising the C(0)R17 protecting group is accomplished as follows using the acyl chloride (R,7C(0)C1) using 35 HPMPC and cHPMPC as an example 61 Printed from Mimosa 13 07 15 NHBz N O^N NH4OH O TrO' V ,s p-or15 or15 n O^N nh2 r17coci Pyridine "I TrO' p~or15 or15 NHC(0)R N O^ N 17 JO o TrO' ,o p."—OR15 OR15 nhc(0)r 17 N o^^N 1) Delritylation 2) TMSBr ^P: OTr O 11 -or15 "OR 1) Dotritylation 2) NaH/THF N O^N nhc(0)r <?• 17 o II ck ^P- OH -oh oh DCC / Morpholine Pyridine nhc(0)r17 nhc(0)r17 wherein Tr is the hydroxyl protecting group trityl The detntylation step is accomplished by acid treatment, such as 80% acetic acid at about 10* to 60*C for 1-2 hours The R15 moiety is removed using a Lewis acid such as TMSBr to yield the free phosphonate Phosphonate compounds comprising B1 and a C2-20 1-acyloxy-l-alkyl or a 10 C4-20 1-acyloxy-i-alkyl-l-aryl ester group are prepared as follows 62 Printed from Mimosa 13 07 15 NHC(0)R17 NHC(0)R17 Rltt-C(0)-0-CH2Cl O' N,N-Dicyclohexyl-N- ■ morpholine carboxarrudine DMF OH 0CH20C(0)R18 wherein R18 is Ci.2o alkyl which is unsubstituted or substituted by substituents independently selected from the group consisting of Ci-6 alkyl, Ci_6 alkoxy, Ci-6 5 haloalkyl (1 to 3 halogen atoms), cyano, rutro, OFI, O, NH and halogen (including ethyl, propyl, isopropyl, t-butyl, isobutyl and adamantoyl), or C3.10 aryl which is unsubstituted or substituted by substituents independently selected from the group consisting of Cj-6 alkyl, Ci-6 alkoxy, Ci-6 haloalkyl (1 to 3 halogen atoms), cyano, mtro, OH, O, N and halogen (including phenyl, and 3- or 4-10 pyridyl) The amine protecting group =CR19N(R20)2, where R19 is hydrogen or CH3 and R20 is C1-10 alkyl, or both R2() together are 1-morpholino, 1-piperidine or 1-pyrrohdine, is incorporated into an exocychc amine to yield protected heterocyclic base compounds as follows Exemplary R20 alkyl groups include methyl, ethyl, propyl, isopropyl, cyclopropyl, butyl, isobutyl, t-butyl and cyclobutyl In general, both R20 alkyl groups will be the same The reaction can be carried out in dry DMF at room temperature 20 (about 20-30'C) as previously described (Kerr et al T Pharm Sci (1994) £2 582, Kerr et a! T. Med. Chem (1992) 251996), or DMF can be substituted with CH3CN NH2 N=CR19N(R2% O" ^OR15 63 Printed from Mimosa 13 07 15 WO *>7/24361 rCT/US96/20226 and 4 A molecular sieves Protected heterocyclic bases where R19 is hydiogen are stable under neutral anhydrous conditions and are generally labile under acidic aqueous conditions When R19 is methyl, the protecting group is more stable to aqueous acidic or basic conditions Table 3 lists R15 ester and other moieties that can be incorporated into the phosphorus atom of both cyclic moieties (^uch as cHPMPC comprising a protected heterocyclic base or cHPMPC) or linear moieties (such as HPMPC comprising a protected heterocyclic base or PMEA comprising a protected heterocycbc base or PMEA) Esters of Table 3 structures 1-5, 8-10 and 16,17,19-22 are synthesized by reacting a nucleotide analog (such as cHPMPC) the corresponding halide (chloride or acyl chloride and the like) and N ,N-dicylohexyl-N-morpholine carboxamidine (or another base such as DBU, triethylamme, CSCO3, N,N-dimethylaniline and the like) ir DMF (or other solvent such as acetonitrile or N-methylpyrrolidone) Esters of structures 5-7, 11, 12, 21, and 23-26 are synthesized by reaction of the alcohol or alkoxide salt (or the corresponding amines in the case of compounds such as 13,14 and 15) with a nucleotide analog monochlorophosphonate or dichlorophosphonate (such as cHPMPC monochlorophosphonate or PMEA dichlorophosphonate) or another activated phosphonate TABLE 3 1 -CH2-C(0)-N(RC)2 * -CH2-0-C(0)-C(CH3)3 2 -CH2-S(0)(RC) 11 -CH2-CCI3 3 -CH2-S(0)2(RC) 12 -C6H5 4 -C.H2^>C(0)-CH2-C6H5 13 -NH-CH2-C(0)0-CH2CH3 3-cholesteryl 14 -N(CH^)-CH2-C(0)0-CH2CH3 6 3-pyridyl -NHRd* 7 N-ethylrnorpholino 16 -CH2-O-C(O)-C10Hi5 8 -CH2-0-C(0)C6H5 17 -CH2-0-C(0)-CH(CH3)2 9 -CH2-0-C(0)-CH2CH3 18 -CH2-C#H(0C(0)CH2Rc)-CH2- (0C(0)CH2Rcr 19 /—\ -CH2C(0)N^ O HO—| / O. $ 21 ho 64 Printed from Mimosa 13 07 15 ch,0(0"~ 22 -chro-c(0) n n—^ -o ch3ch:0(0^ 26 och3 * - RD is Cj 20 alkyl, including C4.16 alkyl ** - Each RC is the same or different (includes methyl, ethyl, propyl, isopropyl and t-butyl) examples are illustrative and do not limit the scope of this invention Example 1 Synthesis of phosphonate amidate compounds The compounds of structural formula Id shown are in Table 6 (bis(glycyl benzyl ester)PMEA (compound Ex 4), bis(alanyl benzyl ester)PMEA (Ex 1), 15 bis(phenylalanyl benzyl ester)PMEA (Ex 5), etc Compounds Ex 1 - Ex 12 were synthesized by the following procedure PMEA (Z-B = -CH2-O-CH2-CH2-B, where B is adenin-9-yl) (0 3 g, 1 1 mmol) and amino acid ester»HCl (2 2 mmol, Sigma) were suspended in dry pyridine (6 mL) containing trjethylamine (0 3 mL, 22 2 mmol), followed by addition to a mixture of freshly prepared 20 triphenylphosphine (3 3 mmol) and 2,2'-dipyridyl disulfide (3 3 mmol) in pyridine (3 mL) The mixture was stirred at room temperature overnight, concentrated and partitioned between methylene chloride and water The orgaruc solution was dried over MgSC>4, concentrated and purified by flash column chromatography on silica gel 25 Ex 14 was synthesized using freshly prepared triphenylphosphine (6 0 mmol) and 2,2'-dipyridyl disulfide (6 0 mmol) in pyridine (20 mL) at room temperature to which PMEA (2 0 mmol) was added The suspension was stirred for 10 min and ethyl sarcosme HC1 (N-methylglycme HC1 ethyl ester, 1 2 g, 8 0 mmol) was added The suspension was warmed to 90*C and stirred for 24 hours 30 Crude product was concentrated by rotary evaporation and purified by silica flash All citations are hereby expressly incorporated by reference The following 65 Printed from Mimosa 13 07 15 WO 97/24361 PCTAIS96/20226 chromatography (mobile phase 1% methanol gradient to 20% methanol/80% methylene chloride) Compound Ex 13 was. synthesized in a similar manner using PMEA and phenylalanine N-ethylmorpholino ester TABLE 6 Compound U Ex 1 -NH-CH(CH3)-C(0)0CH2C6H5 Ex 2 -NH-CH(CH2C6H5K(0)OCH2C6H5 Ex 3 -NH-CH(CH2CH(CH3)2)C(0)0CH2C6H5 Ex 4 -NH-CH2-C(0)0CH2QH5 Ex 5 -NH-CH(CH3)-C(0)0C2H5 Ex 6 -NH-CH(CH2CH(CH3)2)-C(0)0C2H5 Ex 7 -NH-CH2-C(0)0C2H5 Ex 8 -NH-CH(CH2C6H5)-C(0)0C(CI I3)3 Ex 9 -NH-CH(CH2CH(CH3)2)-C(0)0C(CH3)3 Ex 10 -NH-CH(CH3)-C(0)0C(CH3)3 Ex 11 -NH-CH2-C(0)0C(CH3)3 Ex 12 -NH-CH(CH2C6H5)-C(0)0C2H5 Ex 13 -NH-CH(CH2C6H5)C(0)0-(CH2)2-N[(CH2)2(CH2)2]0 Lx 14 -N (CH3)-CH2-C (0)0C2H5 Example 2 Antiviral activity Compounds were individually tested for activity against HSV-1 and/or HSV-2 HSV-2 (strain 414-92) was tested using MA 25 104 cells in the following assay protocol 96-Well plates were seeded with 1 x 104 MA 104 cells per well using 200 |iL minimal essential medium (MEM) containing 10% calf serum per well, and incubated overnight at 37*C The compounds were dissolved in MEM Earle's Salts without serum The medium was removed by aspiration and 100 fiL MEM Earle's Salts without serum was 30 added to the wells Serial 3-fold dilutions of the compounds were prepared by serial transfer of 50 jiL of medium from wells containing compound to wells lacking compound The plates were incubated 15 minutes at 37"C followed by addition of 100 PFU/well of virus in MEM Earles Salts with 2% fetal bovine serum The plates were then incubated at 37*C for three days until 35 approximately 90% of the cells in virus infected control wells containing no compound were killed Following incubation, medium was aspirated and the wells were washed with sterile PBS 100 (.iL 0 5% crystal violet in 20% methanol 66 Printed from Mimosa 13 0715 was then added to the wells for 5 minutes, aspirated and the wells were washed tvvo or three times with distilled water 200|iL of 0 01 N HC1 was added to the wells and the absorbance of each well at 595 nm was determined The results, shown in Table 6, were expressed as the ICso, the concentration (nM) that 5 inhibits cell killing mediated by HSV-2 by 50% IC50 values varied from 2 jiM to .>100 compared to an IC50 for PMEA of 21 Thus, some of the compounds were more active against HSV-1 than PMEA The toxicity of the compounds were expressed as the CC50, the concentration that kills 50% of uninfected cells The compounds were also tested for activity against the KOS strain of 10 HSV-1 in VERO cells The results, shown in Table 7, were expressed as the EC50, the concentration (jiM) that inhibits cell killing mediated by HSV-2 by 50% EC50 values varied from 2 pM to >200 |iM compared to an EC50 for PMEA of 138 jiM Thus, some of the compounds were more active against HSV-2 than PMEA TABLE 7 HSV-1 HSV-2 compound EC50 IC50 CC50 Ex 7 >200 >100 >100 Ex 5 nt* >100 >100 Ex 6 33 >100 Ex 12 nt 80 Ex 11 >200 >100 >100 Ex 10 >200 >100 >100 Ex 9 63 63 >100 Ex 8 3 9 Ex 4 nt 60 >100 Ex 1 nt >100 Ex 3 nt 2 Ex 2 nt 4 * nt - not tested Example 3 PMEA. monophenyl ester, mono N-ethylmorpholino-20 phenvlalanyl phosphoroamidate Bis(phenyl)PMEA is selectively hydrolyzed to the monophenyl ester of PMEA using NaOH in THF The reaction mixture is neutralized with acid (1 NT HC1), and the monophenyl PMEA is isolated by 67 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 filtration The anhydrous monophenyl PMEA and 2 equivalents of a freshly prepared 11 mixture of triphenylphosphine and 2,2'-dipyndyl disulfide in pyridine is condensed with 1 equivalent of phenylalanine N-ethyl-morphohno ester in triethylamine and pyridine to afford the title compound The title 5 compound is recovered by evaporation of the solvents under reduced pressure and purified by silica gel chromatography Example 4 Antiviral activity of PMEA esters PMEA and PMEA esters were tested for inhibition of cytopathic effects by HSV II in MA 104 cells as described except that CPE was determined after incubation with virus by addition 10 of 100|iL XTT, 1 mg/mL in deficient DME containing 25 |iM PMF followed by measuring absorbance The esters tested were bis(POM)PMEA, bis(phenyl)PMEA, monophenylPMEA, bis(3-dimethylaminophenyl)PMEA, bis(3-methoxyphenyl)PMEA, bis(2-carboethoxyphenyl)PMEA, bis(adamantoyl oxymethyl)PMEA, bis(4-fluorophenyl)PMEA and bis(2-ethoxyphenyl)PMEA All 15 of the compounds tested were active, which indicated that the ester groups were removed, thereby allowing free PMEA to inhibit virus replication and/or cytopathic effects The IC50 and CC50 of PMEA in the assay was 19 3 nM and 2000 |iM respectively and the IC50 and CC50 of bis(POM)PMEA m the assay was 0 5 jxM and >10 |iM respectively IC50 values for the mono and bis esters ranged from 1 1 20 (iM to 67 5 nM and the CC50 values ranged from 70 |iM to 500 jiM Example 5 Oral bioavailability of nucleotide analog anrudates and PMEA esters Nucleotide analog amidates and nucleotide analogs are tested for their bioavailability when administered to cynomologous (or rhesus) monkeys by oral, subcutaneous or intramuscular routes Bioavailability is determined by 25 measuring PMEA levels m plasma or urine at different times after administering the drug using radiolabeled (3H, 14C, etc) compound or, for compounds having adenine, essentially as described (Naesens, et al, Clin Chem (1992) 480-485, Russell, et al, I Chromatogrr (Netherlands) (1991) 572 321-326) Radiolabeled compounds are obtained commercially (Moravek Biochemicals, Brea, CA) or by 30 standard procedures, such as catalytic hydrogen exchange for 3H labeling Compounds such as bis(2-ethoxyphenyl)PMEA, bis(2-carboethoxyphenyl)PMEA, bis(0-benzylphenylalanyl)PMEA, bis(3,5-dimethoxyphenyl)PMEA, bis(4-fluorophenyl)PMEA, bis(adamantoyl oxymethyl)PMEA, bis(phenyl)PMEA, bis(3-methoxyphenyl)PMEA are tested foi oral bioavailability by administering about 35 10 - 30 mg/Kg (usually 15 to 25 mg/Kg) containing about 20 - 50 nCi/Kg (usually about 40 |iCi/Kg) of radiolabeled compound, followed by withdrawing blood samples at several times after administration (exemplary time points are 0 1, 0 25, 68 Printed from Mimosa 13 07 15 0 5, 1 0,1 5, 2 0, 2 5, 3 0, 4, 6, 12,18, 24, 36, 48, 72, 96 hours after administration), obtaining plasma and determining the amount of radiolabeled compound present per volume (about 0 1-10 mL) of serum Oral bioavailability of the tested compounds is 2 - 80% (or any value between 2% and 80% in 1% 5 increments), preferably 10 - 80% and more preferably 15 to 80% The oral bioavailability of bis(POM)PMEA by this type of assay is typically about 25% in monkeys and PMEA is about 2 - 4% (Balzanru et al, Animal Models in AIDS (1990) p 131-138, Schellekens, H et al (ed), Elsevier Science Publications, Amsterdam) while nucleotide analog amidates and nucleotide analogs 10 (including mono- and diesters) can have oral bioavailabilities of about 5%, 10%, 15%, 30%, 40%, 50%, 60% or 80% Total radioactivity in plasma is determined by mixing about 200 |iL of plasma with a scintillation counting cocktail (such as 10 mL of Scinti-Safe plus LSC cocktail) and counting in a scintillation counter (usually for about 5-30 15 minutes) Detailed analysis of the radiochemical composition is accomplished using about 350 (iL of plasma, denaturing proteins in the serum (using about 700 (iL 0 1% trifluoroacetic acid in acetorutrile for example), drying the resulting sample under reduced pressure, suspending the sample in an appropriate buffer (for example using about 100 ^L of 2% acetorutrile in 25 mM potassium 20 phosphate buffer with 10 mM tetrabutyl ammonium hydrogen phosphate (TBAHP), pH 6 0 for HPLC analysis), centrifuging the sample and analyzing the supernatant for individual radiolabeled species by reverse phase HPLC on commercially available columns (The Separation Group, Hesperia, CA, Vydac C18, 5 |im, 250 x 4 6 mm column with an injection volume of about 50 p.L and a 25 flow rate of about 1 0 mL/min at about 35*C using buffer for 2 minutes followed by a linear gradient to about 65% acetonitrile m 25 mM potassium phosphate buffer with 10 mM TBAHP, pH 6 0 over 13 about minutes) Radiolabel detection is accomplished using means such as commercially available radioactive flow detection systems or scintillation counting systems (Packard, Meridian, CT) 30 Fluorescence detection of PMEA in plasma is accomplished by measuring fluorescence emission (420 ran, with excitation at about 236 nm) with a detector (model F2000, Spectra Physics, San Jose, CA) from the HPLC gradient essentially as described above (2 to 65% acetonitrile) Samples for analysis are prepared from plasma (200 p.L) by protein precipitation with TFA (400 jiL 0 1°/.. in acetorutrile), 35 drying and conversion of adenine to N6-ethenoadenine in 200 |iL of reaction buffer (0 34% chloroacetaldehyde, 100 mM sodium acetate, pH 4 5) for 40 minutes at 95"C followed by HPLC analysis using 50 jxL 69 Printed from Mimosa 13 07 15 Example 6 Bisfadamantovl oxvmethvnPMEA ester DBU (1,8-diazabicyclo[5 4 0)undec-7-ene, 1 53 g, 10 mmol) was added to a suspension of PMEA (1 365 g, 5 mmol) in DMF (25 mL) Adamantoyl oxymethyl chloride (5 72 g, 25 mmol) m DMF (25 mL) was added to the reaction mixture which was tnen 5 stirred for four days at room temperature and the volatiles were removed under vacuum The crude product obtained after removal of the solvent was loaded onto a silica gel column and washed with 3% MeOH/CH2Cl2 to remove nonpolar impurities 1 g (30%) of bis(adamantoyl oxymethyl)PMEA ester was eluted m 8% MeOH/CH2Cl2 Adamantoyl oxymethyl chloride was obtained by 10 conversion of 1-adamantanecarbonyl chloride (Aldrich No 11,772-2) with (CH20)n/ZnCl2 and has been described (Bodor, et al T Med Chem (1980) 23. 474-480) Example 7 BisfphenvHPMFA and bisf2-ethoxvphenv]VPMEA esters PMEA (2 0 g, 7 3 mmol), acetonitrile (20 mL), thionyl chloride (20 mL) and N,N-15 dimethylformamide (2 drops) were added to a 250 mL single neck round bottom flask equipped with a magnetic stirrer, water cooled condenser and N2 atmosphere The flask was immersed in a 85*C oil bath and the resulting suspension was stirred for two hours The resulting solution was then concentrated to dryness and acetorutrile (50 mL) was added to redissolve the 20 crude chlondate To a separate 250 mL single neck round bottom flask equipped with a mechanical stirrer, and N2 atmosphere, phenol (3 25 g, 35 mmol), tetrahydiofuran (80 mL) and sodium hydride (1 4 g, 34 mmol, 60% (w/w) dispersion m mineral oil) was charged After stirring for 30 minutes, the 25 solution was cooled to -78*C with a dry ice-acetone bath The acetonitrile from the previous step was then added drop-wise at a rate that the internal temperature did not rise above -76*C After the addition was complete, the resulting suspension was poured into saturated aqueous NaFICC>3 (100 mL) and extracted with methylene chloride (3 x 150 mL) The combined orgaruc extracts 30 were washed with H2O (100 mL), brine (100 mL) and dried with anhydrous Na2SC>4 Concentration by rotary evaporation afforded a yellow solid Purification by recrvstallization (ethyl acetate/hexanes) afforded pure bis(phenyl)PMEA (1 64 g, 53%) Bis(2-ethoxyphenyl)PMEA was made similarly using 2-ethoxyphenol in place of phenol in 36% yield 35 Example 8 (R)-9-(2-Di-2 ethoxyphenylphosphonylmethoxypropvn adenine To a solution of 2-ethoxyphenol (45 mmol, 6 22 g) in pyridine (75 mL) was added (R)-9-(2-phosphonylmethoxypropyl adenine (PMPA, 15 mmol, 4 3 g), 70 Printed from Mimosa 13 07 15 creating a white suspension A separate solution of 2,2'-dipyndvl disulfide (45 mmol, 9 91 g) and tnphenyl phosphine (45 mmol, 11 81 g) in pyridine (75 mL) was added at 22°C in a single portion to the white suspension Then, tnethylamine (30 mmol, 4 18 mL) was added in a single portion to the entire 5 mixture, which was stirred at 75°C for 21 h (TLC 10% MeOH/EtoAc) The dark amber slurry was then coevaporated with toluene (100 mL) It was then dissolved m dichloromethane (200 mL) and extracted twice with water (200 mL) The organic phase was dried (NaS04), filtered and concentrated (m vacuo) to a brown syrup (25 4 g) The syrup was purified by flash chromatography 1-5% 10 MeOH/EtoAc to elute impurities, then 6-12% MeOH/EtoAc (title compound elutes at 10-11%) The desired fractions were concentrated to afford 1 04 g of a brown solid The solid was then recrystalized (EtoAc) to give the title compound (780 mg, 12% yield) as a tan solid HNMR (CDC13) 5 1 25 (d, J=7 5Hz, 3H, CH3), 6 1 46 (m, 6H (OCH2CHi)2), 4H (OCH2CH3)2), 8 3 9 (m, 2H, 0-CH2P), 8 4 04 (m, 1H, 15 H-2'), 5 4 09-4 39 (m, 2H, H-l"), 7 24 (m, 8H, (QHjh), 7 92 (5,1H, (C«-H), 8 19 (S, 1H, C2-H) Example 9 cHPMPU cHPMPU was synthesized by adding thionyl chloride (60 mL, 0 812 mmol, 2 02 eq) dropwise to a suspension of disodium HPMPU (131 mg, 0 404 mmol) in N,N-dmethylformamide (1 25 mL) at ambient temperature 20 The resulting light-yellow solution was stirred for 20 mm at ambient temperature and then concentrated to dryness (in vacuo, 45 °C) H20 (2 mL) was added and the resulting solution was concentrated to dryness Methanol (4 mL) was added and the resulting solution was concentrated to dryness to afford the crude product as a light-yellow solid Purification by silica flash chromatography 25 (mobile phase 30% methanol 70% CH2CI2 gradient to 50% methanol 50% CH2CI2) afforded pure cHPMPU in 69% yield as a white amorphous solid *H NMR (300 MHz, D20) d 7 62 d (1H, / = 7 1 Hz, CH=CH), 5 82 d (1H, / = 7 8 Hz, CH=CH), 4 30-3 71 m (7H, CH2CH(0CH2P)CH20H), NH and OH not observed in D20 13c NMR (75 MHz, D20) d, 169 6 s (4-C), 155 1 s (2-C), 150 4 s (6-C), 104 2 s (5-30 C), 76 71 d (JP,C = 3 6 Hz, 2'-CH2), 72 30 d (JP,C = 6 2 Hz, 3'-CH2), 67 90 d (JP,C= 142 0 Hz, P-CH2), 50 71 s (l'-C) 31p NMR (121 MHz, D2O) d 9 23 s Example 10. cHPMPC ethyl ester To a stirred solution of diethyl HPMPC (1 lg) in DMF, NaH (115 mg) was added After 15 mm, the reaction mixture was quenched with acetic acid (1 eq) The solvents were removed under reduced 35 pressure The crude mixture was dissolved in CH2C12 and water The organic layer was washed with NaCl solution and the crude material obtained was 71 Printed from Mimosa 13 07 15 PCMJS96/20226 purified on a silica gel column (elution with 5%-10% MeOH in CH2CI2) to get cyclic ethyl HPMPC (950 mg) as a diastereomenc mixture (approximately 70%) Example 11 cHPMPC esters To a stirred suspension of HPMPC (2 79 g) in DMF, thionylchlonde (2 1 mL) was added drop wise under anhydrous conditions and the mixture was sbrred for 1 hr In another flask, sodium aryloxide (using the appropriate aryl substituent) was made using the correspondmg phenol (8 9 g) and NaH (1 8 g) in 10 11 DMF/THF (50 mL) This solution was cooled to -78*C and the chlondate solution was added dropwise under anhydrous conditions After 2 hrs, the reaction mixture was quenched with acetic acid (5 eq) and the solvents were evaporated under vacuum The crude mixture was partitioned between water and CH2CI2 The organic layer was concentrated and the residue was purified on 15 a silica gel column (elution with 5%-10% MeOH in CH2C12; to get the cyclic aryl compound as a single diastereomer in approximately 60% yield This method is suitable for all substituted or unsubstituted R15 groups, especially aryl, subject of course to conventional protection of labile groups other than ammo for which reaction is undesired (amino is protected by reaction with DMF and deprotected 20 with acetic acid and alkanol treatment) This method offers the advantages of producing substantially stereochemically pure product, superior yield and ease of synthesis .

RT -78°C MeOH OR15 72 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 Example 12. cHPMPC esters NFH, ,16 O^Cl N,N'-dicycIohexyl-4-morpholinecarboxamidine R16 = t-Bu R16 = Adamantyl ° OCH2C(0)R16 To a stirred suspension of cyclic HPMPC (1 mmol) was added N,N'-5 dicyclohexyl-4-morpholinecarboxamidine (2 mmol) followed by the corresponding acyloxymethyl chloride (1 5 mmol) The reaction was stirred for 3 days and the DMF was evaporated under reduced pressure The crude was purified on a silica gel column (eluted with 5% methanol in methylene chloride) to get the pure cyclic HPMPC derivatives (approximately 30% yield) 10 The final product was obtained in higher yield by the same reaction using cyclic HPMPC (1 mmol), N,N'-dicyclohexyl-4-morphohne-carboxarmdine (1 1 mmol) followed by the corresponding acyloxymethyl chloride (1 2 mmol) N4-benzoyl cHPMPC pivaloyloxymethyl ester was synthesized in a similar manner using N4-benzoyl cHPMPC as the starting material 15 Example 13 cHPMPC esters cHPMPC esters were synthesized using appropriate reactants essentially as described in Example 11 for ester moieties corresponding to structure numbers 6, 7, 11,12,13, 23,24, 25 and 26 in Table 3 cFIPMPC esters were synthesized using appropriate reactants essentially as described in Example 12 for ester moieties corresponding to structure numbers 8, 20 9, 10, 16 and 17 in Table 3 Melting point data for cHPMPC esters of compound numbers 6,8,9, 11, 24, 25 and 26 was as follows cHPMPC 3-pyridyl ester (#6) - 268-273*C (decomposes), cHPMPC N-ethylmorphohno ester (#7) - 241'C, cHPMPC -CH2-0-C(0)-CfiH5 ester (#8) - 198-201*C, cHPMPC #9 ortho ester - 176*C, cHPMPC #11 ester - 100-250"C (decomposes), cHPMPC phenyl ester (#12) - 190'C, 25 cHPMPC #24 ester - 218-225"C (waxy liquid), cHPMPC #25 ester - 171'C, cHPMPC #26 ester - 181*C 73 Printed from Mimosa 13 07 15 Example 14 9-f2.3-dideoxy-2.3-didehydro-4-phosphonomethoxy-B-D-ervthrofuranosvllndemne esters Compounds were synthesized by addition-elimination reaction using -°- -B , where B was adenine with iodine (2 equivalents) in acetonnrile and a compound having the structure (R150)2P(0)-CH2-0H (where R15 was isopropyl, phenyl or 2-ethoxyphenyl) to yield the 3-iodophosphonate (R,50)2P(OK O^ /O b diester, I , which was then eliminated to yield the corresponding structure V compound by reaction with 5 equivalents of sodium methoxide or DBU in anhydrous organic solvent such as methanol or 10 tetrahydrofuran at room temperature for 12 hours Compounds of structure (R15O)2P(0)-CH2-OH where R15 was phenyl or 2-ethoxyphenyl were obtained by reaction of 1 equivalent of PCI3 with 1 equivalent of t-butanol at 55" C to obtain (R15OhP(0)H (U S Patent 3,329,742) (R150)2P(0)H was then silylated using 1 equivalent of bis(trimethylsilyl)-tnfluoroacetamide 15 and the resulting (RlsO)2P(OTMS) was dried under vacuum (RlsO)2P(OTMS) was then converted to (R15)2P(0)-CH2-OH by reaction in paraformaldehyde containing catalytic amounts of titanium isopropoxide (or another lewis acid such as titanium tetrachloride and the like can be used) for 12 hrs (12-16 hours) at 70*C (65 to 75"C) The 2-ethoxyphenyl product was isolated by crystallization 20 The bis-phenyl product was isolated by silica gel chromatography bis(2-ethoxyphenyl) D4AMPI ester ^H-NMR (300 MHz, CDCL3) 5 8 38(s, 1H), 7 97 (s, 1H), 7 21-6 82 (m, 9H), 6 40 (d, 1H, J=5 7 Hz), 6 30 (d, 1H, J=5 8 Hz), 6 16 (s, 1H), 5 61 (s, 2H), 4 48 (dd, 1H, J=14,8 8 Hz), 4 38 (dd, 1H, J=14, 6 5 Hz), 4 10-3 93 (m, 4H), 1 38 (t, 3H, J=71 Hz;, 1 35 (t, 3H, J=7 1 Hz), 3*P-NMR (121 MHz, CDCL3) 5 25 14 6 bis (phenyl) D4AMPI ester !H-NMR (300 MHz, CDCL3) 5 8 38 (s, 1H), 7 93 (s, 1H), 7 34 - 7 10 (m, 10H), 7 03 (s, 1H), 6 42 (d, 1H, J=5 6 Hz), 6 34 (d, 1H, J=5 6 Hz), 5 98 (s, 1H), 5 83 (s, 2H), 4 32 (dd, 1H, J=14,6 5 Hz), 4 19 (dd, 1H, J=14,6 5 Hz), 31P -NMR (121 MHz, CDCL3) 5 13 3 30 (C6H4(0C2H5)-0)2P(0)-CH2-0H iH-NMR(300MHz,CDCL3) 5 7 36-716 (m, 10H), 4 19 (dd 2H, J=6 7, 5 9 Hz), OH not detected, 31P -NMR (121 MHz, CDCL3) 6 17 0 74 Printed from Mimosa 13 07 15 PCT7US96/20226 (C6H5-O)2p(0)-CH2-OH 1H-NMR (300 MHz CDCL3) 5 7 25-6 89 (m, 8H), 4 24 (d, 2H, J=5 01 Hz), 4 18-4 08 (m, 4H), 1 46 (t, 6H, J=7 0 Hz), 31P -NMR (121 MHz, CDCL3) 5 19 9 Example 15 Nl-benzovl cHPMPC The title compound was synthesized 5 using N4-benzoyl HPMPC diethyl ester tritylated at the hydroxyl group as a starting material The starting material was detritylated using acetic acid and then converted to N4-benzoyl HPMPC using TMSBr The resulting compound was converted to N4-benzoyl cHPMPC using DCC and morpholine in pyridine The title compound was tested for activity against HCMV in tissue culture 10 (NHDF cell line) and was found to be active with an IC50 of 22 |iM compared with 0 4 |iM for HPMPC 1HNMR (300 MHz, CDCL3) 5 8 02 (H6,1H, d, 7 2 Hz), 7 97 (aromatic, 2H, d, 7 2 Hz), 7 62 (aromatic, 1H, t, 7 2 Hz), 7 5 (aromatic, 2H, t, 7 2 Hz), 7 26 (H5,1H, d, 7 2 Hz), 4 28 (1H, t, 14 7 Hz), 4 15 (1H, t, 10 8 Hz), 4 0 (m, 3H), 3 84(lH,m), 2 49 (1H, 15 d, 14 1 Hz), 31P -NMR (121 MHz, CDCL3) 5 10 07 Melting point 243-246*C Example 16 cHPMPC esters We synthesized formula (1) NPE esters by one of three methods In the first method, ' method A", we added HPMPC (6 74 g, 0 024 mol) to a stirred suspension of Vilsmeier reagent (7 7 g, 0 060 mol) in CH3CN (250 mL) and continued stirring the reaction mixture for three hours 20 We then added a solution of a formula (6) sodium salicylate (0 120 mol, freshly made from the corresponding salicylate and NaH in DMF or in a DMF-tetrahydrofuran (THF) 1 1 (v/v) mixture) to the chloridate reaction mixture at once After 2 hours, the solids were filtered off The sohds were washed with 100 mL CH3CN and then washed with 100 mL CH2CI2 The combined filtrates were concentrated under reduced pressure The crude residue was dissolved in 50 mL CH2CI2 and this was slowly added to 500 mL of diethyl ether with stirnng The solid was collected by filtration and was identified by *H NMR and 31P NMR as 30 about 90% of the equitonal isomer and about 10% of the axial isomer (at the phosphorus atom) of cHPMPC salicylate ester (~ 50-60%), 1 e , a formula (1) compound where all R1 were hydrogen and R was not hydrogen R 75 Printed from Mimosa 13 07 15 WO 97/24361 PCT/IJS96/20226 We converted the equatorial isomer to the axial isomer as follows We dissolved cHPMPC salicylate ester (equitonal isomer, 3 3g, 6 7 mmol) in 30 mL of all (v/v) DMF-THF solvent mixture We then added 7 25 mmol of the corresponding sodium salicylate in 25 mL of DMF (or in 25 mL of a DMF-THF 1 1 5 mixture) to the cHPMPC salicylate ester solution The resulting solution was stirred for 16 hours at r t (room temperature) and then 3 8 mL of glacial acetic acid was added The mixture was stirred for 1 hour and the solvents were removed under vacuum and the crude residue was purified by sihcal gel column chromatography to give predominantly the axial isomer (about 10% of the 10 equatonal isomer and about 90% of the axial isomer at the phosphorus atom) by NMR We synthesized formula (1) NPEs b> "method B" as follows We slowly added oxalyl chloride (3 1 mL, 0 0357 mol) to an ice cold HPMPC suspension (4 g, 14 3 mmol) in CH-^CN (40mL) and DMF (2 8 mL) We warmed the reaction 15 mixture to room temperature and stirred it for 3 hours A solution of formula (6) sodium salicylate (100 mmol, freshly made from NaH and the corresponding salicylic acid ester in DMF or in a DMF-THF mixture) was added to the reaction mixture and stirred overnight at room temperature Glacial acetic acid (7 2 mL) was added to the reaction mixture and stirring was then continued for 1 hour 20 The reaction mixture was then concentrated under reduced pressure The crude residue was suspended in hexane or diethyl ether or a hexane-diethyl ether mixture for 20 minutes The solids were collected by filtration and dissolved in dioxane IN HC1 (80 mL 20 mL) The mixture was stirred for 2 hours at r t and the solvents were then removed under reduced pressure The crude residue was 25 then dissolved in 300 mL CH2CI2 and washed twice with 200 mL water The milky organic layer was dried and concentrated on a rotary evaporator The crude product was a mixture of about 6 4 of axia(axphosphorus atom This material was isomerized as described in method A to obtain about a 9 1 isomer ratio at the phosphorus atom, with the axial isomer as the predominant isomer 30 We synthesized formula (1) NPEs by "method C" as follows We slowly added oxalyl chloride (7 75 mL, 89 3 mmol) to an ice cold HPMPC suspension (10 g, 35 7 mmol) in CH3CN (150mL) and DMF (6 9 mL) We warmed the reaction mixture to room temperature and stirred it for 3 hours A solution of formula (6) sodium salicylate (178 6 mmol, freshly made from NaH and the 35 corresponding salicylic acid ester in THF or DMF or in a DMF-THF mixture) was slowly added to the reaction mixture over a 30 minute period and the mixture was then stirred for 3 hours at r t and the solvents were then removed under 76 Printed from Mimosa 13 07 15 reduced pressure The crude residue was then dissolved in 300 mL CH2CI2 and washod twice with 100 mL water The orgaruc layer was dried and concentrated on a rotary evaporator The crude product was subjected to silica gel chromatography to obtain the salicylate ester of cHPMPC, le, the formula (1) 5 compound, in about a 9 1 isomer ratio at the phosphorus atom with the equatorial isomer predominating The following formula (1) cHPMPC compounds were synthesized - all R1 were hydrogen and R is as designated The desigantion (eq) means the equatorial isomer at the phosphorus atom was present in about 1 9 1 ratio over the axial 10 isomer The desigantion (ax) means the axial isomer at the phosphorus atom was present in about a 9 1 ratio over the equatorial isomer £ method -COO(CH2)3CH3 (eq) A, C -COO(CH2)3CH3(ax) A, B -COO(CH2)4CH3 (ax) B -COO(CH2)5CH3(eq) A -COO(CH2)5CH3 (ax) A, B -COO(CH2)9CH3 (ax) A -COOCH[(CH2)2CH^]2 (ax) A COOCH2CH(C2H5)(CH2)3CH3 (eq) A -COOCH2CH(C2H5)(CH2)3CH3 (ax) A -COOCftHn (ax) A -COOC6H5 (eq) A -COOC6H5 (ax) A -COOC6H4-p-C{CH3b(eq) A -COOC6H4-p-C(CH3)3) A -COOCH2C6H5 (ax) A -COOCH2CH2C6H5 (eq) A -COOCHjCH.C.H^ fax) A.B We obtained the following NMR spectra for the indicated formula (1) compounds (all Rl were hydrogen) R = -C(0)0CH2CH2CfiH5 (equitonal isomer) 'H NMR (300 MHz, CDCI3) 5 35 7 85 (1H, d, J=7 5 Hz), 7 5 (1H, t, J=7 8 Hz), 7 35-7 2 (8H, m), 5 75 (1H, d, 1=7 2 Hz), 4 55-4 35 (5H, m), 4 2-4 1 (2H, m), 4 05 (1H, d, J=14 4 Hz), 3 56 (1H, dd, J1=15 Hz, J2=8 1 Hz), 3 0 (2H, t, J=6 9 Hz) 77 Printed from Mimosa 13 07 15 R = -C(0)0CH2CH2C6H5 (axial isomer) lH NMR (300 MHz, CDC13) 5 7 84 (IH, d, J=7 8 Hz), 7 55-7 2 (9H, m), 5 64 (IH, d, J=7 2 Hz), 4 55 (IH, t, J=10 Hz), 4 50 (2H, t, J=6 9 Hz), 4 5-4 3 (2H, m), 4 15-4 1 (2H, m), 4 0 (IH, dd, h=14 7 Hz, J2=l 8 Hz), 3 52 (IH, dd, Ji=14 4 Hz, J2=7 8 Hz), 3 0 (2H, t, J=7 2 Hz) R = -C(0)0CH2CH(C2H5)(CH2)3CH3 (equatorial isomer) !H NMR (300 MHz) 5 7 9 (IH, d, J=7 8 Hz), 7 52 (IH, t, J=7 S Hz), 7 45-7 2 (3H, m), 5 76 (IH, d, J=6 9 Hz), 4 6-4 4 (3H, m), 4 25-4 1 (5H, mj, 3 59 (IH, dd, Ji=14 1, J2=8 1 Hz), 1 8-1 65 (lH,m), 1 5-1 25 (8H,m), 0 98-0 85 (6H, m) R = -C(0)0CH2CH(C2H5)(CH2)3CH3 (axial isomer) *H NMR (300 MHz) 5 10 7 89 (IH, d, J=7 5 Hz), 7 53 (IH, t, J=7 8 Hz), 7 42 (1H, d, J=8 1 Hz), 731 (IH, d, J=7 2 Hz), 7 26 (IH, t, )=7 35 Hz), 5 63 (IH, d, J=7 2 Hz), 4 65 (IH, t, J=10 8 Hz), 4 53-4 1 (2H, m), 4 25-4 1 (4H, m), 4 0 (IH, dd, Ji=15, J2=l 2 Hz), 3 52 (IH, dd, Ji=13 8, J2=7 5 Hz), 1 75-1 6 (lH,m), 1 5-1 2 (8H,m), 0 94 (3H, t, J=7 5 Hz), 0 917 (3H, t, J=7 2 Hz) R = -C(0)0CH2C6H5 (axial isomer) IH NMR (300 MHz) 5 7 97 (1H, d, J=7 5 15 Hz), 7 49 (IH, t, 7 4 Hz), 7 45-7 2 (8H, m), 5 62 (IH, d, J=7 2 Hz), 5 31 (2H, d, J=12 Hz), 4 54 (IH, t, J=10 5 Hz), 4 5-4 35 (IH, m), 4 27 (IH, dd, Ji=15 Hz, J2=l 2 Hz), 4 15-4 05 (2H, m), 3 94 (IH, d, 1=13 5 Hz), 3 42 (IH, dd, Ji=13 5 Hz, J2=7 5 Hz) R =-C(0)0C6H5 (axial isomer) »H NMR (300 MHz) 5 8 15 (IH, d, J=7 8 Hz), 7 6 (IH, t, 7 5 Hz), 7 65-7 2 (7H, m), 7 0 (IH, d, J=-7 2 Hz), 5 56 (IH, d, J=7 2 Hz), 4 56 20 (IH, k, J=10 0 Hz), 4 5-4 3 (2H, m), 4 2-4 0 (2H, m), 3 95 (IH, d, J=15 Hz), 3 28 (IH, dd, Ji=13 8, J2=7 5 Hz) Example 17 Oral bioavailability of cHPMPC and cHPMPC esters This study examined the oral bioavailability of ctTPMPC and cHPMPC esters in Beagle dogs The study design obtained bioavailabilities of cHPMPC and cHPMPC esters 25 in dogs by comparing the AUC, the area under the plasma concentration time curve, after orally administering cHPMPC or a cHPMPC ester to the AUC obtained with cHPMPC after intravenous administration The study used an aqueous solution of cHPMPC for oral administration containing 10 mg/mL cyclic HPMPC in 0 9% NaCl The dose was 1 0 mL/kg (10 30 mg/kg) Formulations for the cHPMPC esters (10 mg/kg solutions) are described below The study used adult male beagle dogs The dogs were fasted 12-18 hours before dosing and until 6 hours post-dose The average gastric pH of a fasted dog is approximately one pH unit higher than that of human and the study used 35 pentagastrin pretreated dogs to adjust the gastric pH (J Dressman, Pharm Res , (1986) 2 123-131) Pentagastrin lowers the gastric pH to a value consistent with a 78 Printed from Mimosa 13 07 15 WO 97/24361 PCMJS96/20226 fasted human subject (R P Happe, et al, Research in Vet Sci.. (1982) 22 232-239) The study used Peptavlon (pentagastrin, 0 25 mg/mL) (Ayerst Laboratories, Inc, Philadelphia, PA) The dogs received a single intramuscular injection of pentagastrin (6 (ig/kg) 20 minutes before dosing to simulate human gastric pH 5 values The dogs received water ad lib Cach formulation was administered as a single dose The study provided for individual vials of each formulation for each animal The oral solutions were administered by gavage, followed by two 10 mL water washes Animals remained conscious throughout the sample collection period 10 Blood samples (4 0 mL) were collected by direct jugular vein access from each animal into hepannized tubes Blood was processed immediately for plasma by centnfugation at 2000 rpm for 10 minutes Plasma samples were frozen and maintained at < -20°C until analyzed Pooled normal dog plasma was used to prepare standard samples 15 Plasma concentrations of cHPMPC and the tested esters were determined using reverse-phase HPLC with fluorescence detection (Excitation 305 nm, Emission 370 nm) of the fluorescent cytosme base 3,N4-etheno derivative Cytidine 5'-monophosphate (5'-CMP) (Sigma, Cat # C-1006) was an internal standard for the HPLC analysis Plasma (100 jiL) was mixed with 400 ^L of 20 protein precipitation solution (10 mL glacial acetic acid, 190 mL water, 800 mL acetorutrile containing 5'-CMP) and the solution was then centnfuged at 20,000 g for 5 min The supernatant was transferred to another tube and mixed with 100 M-L p hen acyl bromide (Fluka), 0 25 g/mL in acetonitrile to generate the cytosine etheno derivative by incubation at 65'C for 40 min The tubes were then put on 25 ice to quench the reaction The samples were then evaporated to dryness under reduced pressure for < 2 5 hours The residue was dissolved in 100 jiL of reconstitution solution (6 mM dodecyltnetylammomum phosphate (Bodman), 30 mM phosphoric acid in water) and filtered through a 0 45 nM filtration unit (Z-spin) The filtrate was then transferred to autosampler vials (Chromacol) for 30 HPLC analysis The HPLC system comprised a Model P4000 (Thermo Separations, San Jose, CA) solvent delivery system with a Model AS3000 automjector (Thermo Separations) and a Model F-1080 fluorescence detector (Hitachi) A Peak Pro data acquisition system (Beckman, Palo Alto, CA) acquired and stored data from the 35 study The column was an Intersil ODS-2, 4 6 x 150 mm, 5 |iM HPLC column (Metachem) operated at 45"C The mobile phase was degassed 6 mM dodecyltnetylammomum phosphate (Bodman), 30 mM phosphoric acid, pH 2 85 79 Printed from Mimosa 13 07 15

Claims (2)

WO 97/24361 PCT/US96/20226 in 30% acetorutrile A flow rate of 2 0 mL/min and an injection volume of 20 nL was used HPLC grade water was used to prepare all solutions and standards for HPLC analysis The following formula (1) compounds were tested for oral bioavailability 5 in the same manner as that used for cHPMPC measurements The tested esters all consisted of about a 90 10 (w/w) racemic mixture at the phosphorus atom and for all compounds, the same isomer predominated R io Compound # Formulation E 1 (cHPMPC) 1 none 2 2 -COOCH2CH3 3 3 -COOCH2CH2CH2CH3 4 3 -COOCH2CH2CH2CH2CH2CH3 15 5 3 -COOCH2CH(CH2CH3)CH2CH2CH2CH3 6 2 -COOCfiHs 7 3 -COOCfiHn 8 3 COOCH2CH2C6H5 The R group in compound #7 was cyclohexyl and the R group in compound #8 20 was phenethyl The formulations that were used to deliver cHPMPC compounds consisted of the following Formulation #1 0 9% NaCl, #2 PEG 400, #3 20% PEG 400 and 80% aqueous citric acid buffer (50 mM, pH 2 2) The compounds were orally bioavailable as cHPMPC follows Compound #1, 22 5%±11 0, compound #2, 18 5%±5 8, compound #3, 46 3%±9 0, compound #4, 30 5%±4 8, compound #5, 25 34 4%±4 8, compound #6, 27 8%±6 2, compound #7, 22 7%±4 4, compound #8, 35 3%±3 4 Bioavailability of 'he compounds as HPMPC ranged from 12 0%±2 6 to 1 4%±1 2, with the latter value coming from cHPMPC Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 What is claimed is 1 A compound having the structure of formula (1) R and the racemates, salts and solvates thereof, 5 wherein B is a protected or unprotected heterocyclic base, R is hydrogen, alkyl, O-alkyl, -CHO, -CH2CH2C6H5, -C(0)0R2, -C(0)R2, -C(0)N(R3)2 or -S(0)2N(R3)2, each R1 is independently hydrogen, cyano, mtro, halogen, alkyl, O-alkyl, -C(0)0R3, -C(G)R3, -S(0)2OH, -N(R3)2, -CHO or -OH, 10 each R2 and each R3 are independently hydrogen, alkyl, phenyl, alkyl substituted phenyl, -CH2C6H5 or -CH2CH2C6H5, provided that the claim excludes compounds wherein the ring containing R and R1 comprises phenyl, phenyl substituted with 1-4 halogen atoms, phenyl substituted with one or two CN, NO2, OH, Ci-12 alkyl or Ci-12 O-alkyl groups, 15 phenyl substituted with two -C(0)0-C2Hs groups, phenyl substituted with one -N(CH3)2 or -C(0)0-C 1-4-alkyl group, or phenyl substituted with one -C(0)0-C2Hs or -O-C2H5 group and one OH group 20 2 The compound of claim 1 wherein B is structure (a), (b), (c) or (d) R .Ry O^ N (a) R 10 X R, R RW ^r9 N (b) NH (c) N—N \ NH- O or R N=/ (d) \ 12 81 Printed from Mimosa 13 0715 WO 97/24361 PCT/US96/20226 R6 is NH2, NHR7, NHR8, N=CHN(R7)2 or N=C(CH3)N(R7)2, R7 is Ci - C6 alkyl, R8 is a protecting group, each Ry is independently N or CH, R»0 is OH, NH2, NHR7 or NHR8, R» is H, NH2, NHR7 or NHR8, and Rt2 is NH or CH2 3 The compound of claim 2 wherein R is -C(0)-0R2, R1 is hydrogen, and B is cytosin-l-yl, 5-fluorocytosin-l-yl, 6-azacytosin-l-yl, 5-methylcytosin-l-yl, adenin- 9-yl, guanin-9-yl or 2, 6-diammopurin-9-yl 4 The compound of claim 3 wherein R2 has 5-12 carbon atoms 5 The compound of claim 3 wherein R2 has 6-10 carbon atoms 6 The compound of claim 4 wherein R2 has 5 carbon atoms 7 The compound of claim 6 wherein R2 is n-pentyl 8 The compound of claim 7 wherein B is cytosm-l-yl 9 The compound of claim 4 wherein R2 has 6 carbon atoms 10 The compound of claim 9 wherein R2 is n-hexyl 11 The compound of claim 10 wherein B is cytosm-l-yl 12 The compound of claim 4 wherein R2 has 7 carbon atoms 13 The compound of claim 12 wherein R2 is n-heptyl 14 The compound of claim 13 wherein B is cytosin-l-yl 15 The compound of claim 4 wherein R2 has 8 carbon atoms 82 Printed from Mimosa 13 07 15 WO 97/24361 PCT/US96/20226 16 The compound ot claim 15 wherein R2 is n-octyl, cyclooctyl or -CH2CH(CH2CHi)CH2CH2CH2CHT 17 The compound of claim 16 wherein B is cytosin-l-yl 5 18 The compound of claim 4 wherein R2 has 9 carbon atoms 19 The compound of claim 18 wherein R2 is n-nonyl 10 20 The compound of claim 19 wherein B is cytosin-l-yl 21 The compound of claim 4 wherein R2 has 10 carbon atoms 22 The compound of claim 21 wherein R2 is n-decyl 15 23 The compound of claim 22 wherein B is cytosin-l-yl 24 The compound of claim 1 wherein R2 is alkyl having more than 5 carbon atoms 20 25 A compound having the structure of formula 26 The compound of claim 1 wherein the carbon or the phosphorus atom chiral center * is stereochemically enriched or resolved as the (R) or (S) enantiomer 30 27 The compound of claim 1 wherein the carbon atom chiral center * is stereochemically resolved as the (S) enantiomer C(0)0(CH2)3CH3 wherein B is cytosin-l-yl and the racemates, salts and solvates thereof 25 83 Printed from Mimosa 13 07 15 ' IT" * t, 1 " '^ The compouna or claim 1 wherein the phosphorus atom crural center-"" 'V stereochemicallv enriched as the (R) or (SI enantiomer 29 The compouna or claim 26 which contains at the pnospnorus atom cnirai center *, 55% to 100% of the (R) enantiomer 30 The compound or claim 2b wruch contains at the phosphorus atom chirai center *, 55% to 100% of the (S) enantiomer 31 The use or a compound ot claim 1 tor the manuracture or a medicament >or the treatment or a viral inrection 32 The use or a compound or claim 27 ror tne manuracture or a medicament ror the treatment or a vrai injection 33 The use or a compound or claim 30 tor the manufacture or a medicament tor the treatment or a viral inrection 34 A compound having the tormula (Rl50)2-P(0)-CH2-0-CH2-CH2-B (Rl50)2-P(0)-CH:-0-C*HiCH20H)-CH2-B, (Rl50)2-P(0)CH2-0-OH(CH3)-CH2-B ,R15O)2-P(0)-CH2-OC-H(CH2F'-CH2-B/ ■ Ri50)2-P(0)-CH:-0-C?HiCH=CH:)-CH:-B, or (R150)2-Pf0)-CH2-0-C=H(CH2N*3)-CH2-B, wherein, n indicates suDStituents linked to the carbon atom are in the (R), (5) or (R,S) configuration B is a protected or unprotected heteroo ciic base, and R15 mdependentlv is n-pentvlsalicvlphenvl or alkvlsalicvlphenyl (Cg-iz alkvl) 35 The compound or claim 34 wherein B is aderun-9-vl, guamn-9-yl, cvtosin-

1-vl, 2,6-diaminopurin-9-vl, 2-aminopunn-9-vI, 5-methvlcytosin-l-yl or 5-fluorocytosin-l-vl 36 The compound or claim 35 wherein R15 is n-hexvlsalicvlphenvl,

2-ethylhexylsalicvlphenvl or n-octvlsalicvlphenvl 37 The use according to claim 31, 32 or 33, substantially as herein descnbed 38 A compound according to any one of claims 1 to 30 and 34 to 36, substantially as herein described >1 i , y - - L I Jl . , 5 84 RECEIVED