NZ192872A - Pregnancy-determining reagent -chain subunits of human chorionic gonadotropin bound to latex polymer - Google Patents

Pregnancy-determining reagent -chain subunits of human chorionic gonadotropin bound to latex polymer

Info

Publication number
NZ192872A
NZ192872A NZ192872A NZ19287280A NZ192872A NZ 192872 A NZ192872 A NZ 192872A NZ 192872 A NZ192872 A NZ 192872A NZ 19287280 A NZ19287280 A NZ 19287280A NZ 192872 A NZ192872 A NZ 192872A
Authority
NZ
New Zealand
Prior art keywords
hcg
chain
beta
aqueous solution
molar aqueous
Prior art date
Application number
NZ192872A
Inventor
H Hansen
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of NZ192872A publication Critical patent/NZ192872A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

Abstract

1. An immunological diagnostic reagent for the determination of pregnancy by means of HCG determination and latex agglutination containing discrete particles of an immunologically inert latex polymer having a particle size of 0.01 to 0.9 mu m and a specific weight of 0.01 to 1.1, to which human choriongonadotropin (HCG) has been covalently bound via an amide bridge with a carbodiimide coupling agent at a pH of 5-8, characterized in that the HCG is used in the form of its beta-chain, whereby this beta-chain is substantially free from the alpha-chain and whereby this beta-chain has been separated from the alpha-chain by treating partially purified HCG containing 2000-5000 IU/mg of HCG for 1/2 to 2 hours at a pH of 4-5.5 and a temperature of 20-50 degrees C with a chaotropic agent, namely with a 7-10 molar aqueous solution of urea, a 7-10 molar aqueous solution of lithium bromide, a 4.5-6 molar aqueous solution of guanidine hydrochloride or a 2-3 molar aqueous solution of ammonium thiocyanate, subsequently adjusting the pH of the reaction mixture to 7.5 and separating the mixture of HCG/chaotropic agent by ion exchange and/or further conventional methods in accordance with which molecules can be separated on the basis of differences in the elektrostatic charge, and whereby neither the partially purified HCG nor the beta-chain thereof have been purified further.
NZ192872A 1979-02-16 1980-02-14 Pregnancy-determining reagent -chain subunits of human chorionic gonadotropin bound to latex polymer NZ192872A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US1262979A 1979-02-16 1979-02-16

Publications (1)

Publication Number Publication Date
NZ192872A true NZ192872A (en) 1983-03-15

Family

ID=21755912

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ192872A NZ192872A (en) 1979-02-16 1980-02-14 Pregnancy-determining reagent -chain subunits of human chorionic gonadotropin bound to latex polymer

Country Status (12)

Country Link
EP (1) EP0014965B1 (en)
JP (1) JPS55160854A (en)
AT (2) ATE10034T1 (en)
AU (1) AU532375B2 (en)
CA (1) CA1119924A (en)
DE (1) DE3069477D1 (en)
DK (1) DK153590C (en)
ES (1) ES8103843A1 (en)
FI (1) FI72822C (en)
NO (1) NO156546C (en)
NZ (1) NZ192872A (en)
ZA (1) ZA80887B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL63855A (en) * 1981-09-16 1984-10-31 Teva Pharma Method and kit for detecting pregnancy
US4543339A (en) * 1982-03-16 1985-09-24 Oneill Christopher Early pregnancy detection by detecting enhanced blood platelet activation
JPS6157856A (en) * 1984-08-29 1986-03-24 Chemo Sero Therapeut Res Inst Method for measuring hormone
JPS63305251A (en) * 1987-06-05 1988-12-13 Dai Ichi Pure Chem Co Ltd Immunoassay utilizing latex aggregation reaction
JPS64169U (en) * 1987-06-22 1989-01-05

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3992514A (en) * 1972-07-17 1976-11-16 Istituto Farmacologico Serono S.P.A. Radioimmunoassay method for human chorionic gonadotropin in the presence of luteinizing hormone
US4123509A (en) * 1974-12-19 1978-10-31 American Home Products Corporation Pregnancy test
US4140662A (en) * 1977-03-25 1979-02-20 Ortho Diagnostics, Inc. Attachment of proteins to inert particles
CA1101330A (en) * 1977-09-19 1981-05-19 Ernst A. Fischer Immunological material bonded to carboxylated latex polymer and process for making it
US4138214A (en) * 1977-12-19 1979-02-06 American Home Products Corporation Diagnostic test utilizing human chorionic gonadotropin
US4234561A (en) * 1978-02-06 1980-11-18 Research Corporation Antigen for early pregnancy test and contraceptive vaccine

Also Published As

Publication number Publication date
AU5561080A (en) 1980-08-21
CA1119924A (en) 1982-03-16
FI72822B (en) 1987-03-31
ATA83880A (en) 1981-05-15
NO800426L (en) 1980-08-18
DK68480A (en) 1980-08-17
JPS55160854A (en) 1980-12-15
DE3069477D1 (en) 1984-11-29
AT365201B (en) 1981-12-28
ZA80887B (en) 1981-02-25
EP0014965B1 (en) 1984-10-24
DK153590C (en) 1988-12-12
JPS6253773B2 (en) 1987-11-12
DK153590B (en) 1988-07-25
ES489277A0 (en) 1981-03-16
NO156546C (en) 1987-10-07
FI800458A (en) 1980-08-17
ES8103843A1 (en) 1981-03-16
ATE10034T1 (en) 1984-11-15
FI72822C (en) 1987-07-10
NO156546B (en) 1987-06-29
AU532375B2 (en) 1983-09-29
EP0014965A1 (en) 1980-09-03

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