NO800426L - IMPROVED LATEX REAGENT FOR IMMUNOLOGICAL PROVISIONS - Google Patents
IMPROVED LATEX REAGENT FOR IMMUNOLOGICAL PROVISIONSInfo
- Publication number
- NO800426L NO800426L NO800426A NO800426A NO800426L NO 800426 L NO800426 L NO 800426L NO 800426 A NO800426 A NO 800426A NO 800426 A NO800426 A NO 800426A NO 800426 L NO800426 L NO 800426L
- Authority
- NO
- Norway
- Prior art keywords
- hcg
- chain
- beta
- aqueous solution
- molar aqueous
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Endocrinology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Reproductive Health (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Peptides Or Proteins (AREA)
- Materials For Medical Uses (AREA)
- Non-Silver Salt Photosensitive Materials And Non-Silver Salt Photography (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Tires In General (AREA)
- Steroid Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
1. An immunological diagnostic reagent for the determination of pregnancy by means of HCG determination and latex agglutination containing discrete particles of an immunologically inert latex polymer having a particle size of 0.01 to 0.9 mu m and a specific weight of 0.01 to 1.1, to which human choriongonadotropin (HCG) has been covalently bound via an amide bridge with a carbodiimide coupling agent at a pH of 5-8, characterized in that the HCG is used in the form of its beta-chain, whereby this beta-chain is substantially free from the alpha-chain and whereby this beta-chain has been separated from the alpha-chain by treating partially purified HCG containing 2000-5000 IU/mg of HCG for 1/2 to 2 hours at a pH of 4-5.5 and a temperature of 20-50 degrees C with a chaotropic agent, namely with a 7-10 molar aqueous solution of urea, a 7-10 molar aqueous solution of lithium bromide, a 4.5-6 molar aqueous solution of guanidine hydrochloride or a 2-3 molar aqueous solution of ammonium thiocyanate, subsequently adjusting the pH of the reaction mixture to 7.5 and separating the mixture of HCG/chaotropic agent by ion exchange and/or further conventional methods in accordance with which molecules can be separated on the basis of differences in the elektrostatic charge, and whereby neither the partially purified HCG nor the beta-chain thereof have been purified further.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US1262979A | 1979-02-16 | 1979-02-16 |
Publications (3)
Publication Number | Publication Date |
---|---|
NO800426L true NO800426L (en) | 1980-08-18 |
NO156546B NO156546B (en) | 1987-06-29 |
NO156546C NO156546C (en) | 1987-10-07 |
Family
ID=21755912
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO800426A NO156546C (en) | 1979-02-16 | 1980-02-15 | IMMUNOLOGICAL REAGENT FOR DETERMINATION OF PREGNANCY, AND PROCEDURE FOR THE DETERMINATION. |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0014965B1 (en) |
JP (1) | JPS55160854A (en) |
AT (2) | ATE10034T1 (en) |
AU (1) | AU532375B2 (en) |
CA (1) | CA1119924A (en) |
DE (1) | DE3069477D1 (en) |
DK (1) | DK153590C (en) |
ES (1) | ES489277A0 (en) |
FI (1) | FI72822C (en) |
NO (1) | NO156546C (en) |
NZ (1) | NZ192872A (en) |
ZA (1) | ZA80887B (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL63855A (en) * | 1981-09-16 | 1984-10-31 | Teva Pharma | Method and kit for detecting pregnancy |
US4543339A (en) * | 1982-03-16 | 1985-09-24 | Oneill Christopher | Early pregnancy detection by detecting enhanced blood platelet activation |
JPS6157856A (en) * | 1984-08-29 | 1986-03-24 | Chemo Sero Therapeut Res Inst | Method for measuring hormone |
JPS63305251A (en) * | 1987-06-05 | 1988-12-13 | Dai Ichi Pure Chem Co Ltd | Immunoassay utilizing latex aggregation reaction |
JPS64169U (en) * | 1987-06-22 | 1989-01-05 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3992514A (en) * | 1972-07-17 | 1976-11-16 | Istituto Farmacologico Serono S.P.A. | Radioimmunoassay method for human chorionic gonadotropin in the presence of luteinizing hormone |
US4123509A (en) * | 1974-12-19 | 1978-10-31 | American Home Products Corporation | Pregnancy test |
US4140662A (en) * | 1977-03-25 | 1979-02-20 | Ortho Diagnostics, Inc. | Attachment of proteins to inert particles |
CA1101330A (en) * | 1977-09-19 | 1981-05-19 | Ernst A. Fischer | Immunological material bonded to carboxylated latex polymer and process for making it |
US4138214A (en) * | 1977-12-19 | 1979-02-06 | American Home Products Corporation | Diagnostic test utilizing human chorionic gonadotropin |
US4234561A (en) * | 1978-02-06 | 1980-11-18 | Research Corporation | Antigen for early pregnancy test and contraceptive vaccine |
-
1980
- 1980-02-14 DE DE8080100755T patent/DE3069477D1/en not_active Expired
- 1980-02-14 EP EP80100755A patent/EP0014965B1/en not_active Expired
- 1980-02-14 AT AT80100755T patent/ATE10034T1/en not_active IP Right Cessation
- 1980-02-14 NZ NZ192872A patent/NZ192872A/en unknown
- 1980-02-14 CA CA000345609A patent/CA1119924A/en not_active Expired
- 1980-02-15 FI FI800458A patent/FI72822C/en not_active IP Right Cessation
- 1980-02-15 DK DK068480A patent/DK153590C/en active
- 1980-02-15 AT AT0083880A patent/AT365201B/en not_active IP Right Cessation
- 1980-02-15 JP JP1669980A patent/JPS55160854A/en active Granted
- 1980-02-15 AU AU55610/80A patent/AU532375B2/en not_active Ceased
- 1980-02-15 ZA ZA00800887A patent/ZA80887B/en unknown
- 1980-02-15 ES ES489277A patent/ES489277A0/en active Granted
- 1980-02-15 NO NO800426A patent/NO156546C/en unknown
Also Published As
Publication number | Publication date |
---|---|
JPS6253773B2 (en) | 1987-11-12 |
EP0014965B1 (en) | 1984-10-24 |
DK153590C (en) | 1988-12-12 |
ES8103843A1 (en) | 1981-03-16 |
FI800458A (en) | 1980-08-17 |
AU532375B2 (en) | 1983-09-29 |
ES489277A0 (en) | 1981-03-16 |
ZA80887B (en) | 1981-02-25 |
AU5561080A (en) | 1980-08-21 |
DK68480A (en) | 1980-08-17 |
ATA83880A (en) | 1981-05-15 |
JPS55160854A (en) | 1980-12-15 |
ATE10034T1 (en) | 1984-11-15 |
NO156546C (en) | 1987-10-07 |
NO156546B (en) | 1987-06-29 |
DE3069477D1 (en) | 1984-11-29 |
FI72822C (en) | 1987-07-10 |
DK153590B (en) | 1988-07-25 |
EP0014965A1 (en) | 1980-09-03 |
CA1119924A (en) | 1982-03-16 |
AT365201B (en) | 1981-12-28 |
NZ192872A (en) | 1983-03-15 |
FI72822B (en) | 1987-03-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US4108974A (en) | Radioimmunoassay for thyroid hormone | |
Funakoshi et al. | Human Carbonic Anhydrases: I. Isolation and demonstration of isozymes in erythrocytes | |
NO800426L (en) | IMPROVED LATEX REAGENT FOR IMMUNOLOGICAL PROVISIONS | |
EP0379133B2 (en) | Use of divalent cations in immunochemical tests | |
EP0266686B1 (en) | Latex agglutination method for the detection of an antibody against streptococcus deoxyribonuclease b | |
HU186928B (en) | Immunochemical reagent preparation | |
Gheţie et al. | Multivalent hybrid antibody | |
US4066410A (en) | Thyroid hormone assay | |
CA1060794A (en) | Composition and method for the determination of pregnancy | |
Pressman et al. | Retention of rabbit antibody activity during acetylation | |
ATE12316T1 (en) | PROCEDURE FOR BINDING AND DISCONNECTING THE COMPETITIVE RADIOIMMUNOASSAY AND REAGENT THEREOF. | |
Ransom et al. | The role of serum proteins in gel-precipitin patterns of Pasteurella pestis | |
SU1158029A3 (en) | Method of quantitative determination of triiodothyronine | |
Solheim | Kinetics of the Reaction between a Monoclonal IgM Anti‐I and Rabbit Red Cells: II. Activity of Subunits and Fab′ μ‐Fragments. | |
SU668712A1 (en) | Collector for flotation of sulfide antimony ores | |
SE8601645D0 (en) | IMMUNOLOGICAL PROCEDURE | |
GB1502823A (en) | Reagent component to be used in radio-immunological determination methods | |
Harms | Studies on the blood proteins of hyperimmune horses. I.—The effect of high concentrations of phenolic substances on the proteins of plasma | |
Veynblat et al. | Suspension diagnosticum for studies on plague bacillus endotoxin and homologous antibodies. | |
Eisentraut | Hormone assay | |
Hohman | The interaction of anti-AMP antibody with adenylylated glutamine synthetase from Escherichia coli |