NO980837L - Identifisering av baser i nukleinsyresekvenser - Google Patents

Identifisering av baser i nukleinsyresekvenser

Info

Publication number
NO980837L
NO980837L NO980837A NO980837A NO980837L NO 980837 L NO980837 L NO 980837L NO 980837 A NO980837 A NO 980837A NO 980837 A NO980837 A NO 980837A NO 980837 L NO980837 L NO 980837L
Authority
NO
Norway
Prior art keywords
primer
sequence
extension
specified position
labeled portion
Prior art date
Application number
NO980837A
Other languages
English (en)
Other versions
NO980837D0 (no
Inventor
Stephen Minter
Original Assignee
Tepnel Medical Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tepnel Medical Ltd filed Critical Tepnel Medical Ltd
Publication of NO980837D0 publication Critical patent/NO980837D0/no
Publication of NO980837L publication Critical patent/NO980837L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Det er beskrevet en fremgangsmåte for å bestemme om en bestemt base er tilstede ved en spesifisert posisjon i en nukleinsyresekvens. Fremgangsmåten omfatter trinnene (a) tilveiebringing av en enkelttrådet prøve av sekvensen som skal bli undersøkt ("prøvesekvens") i immobilisert form på en fast bærer, (b) hybridisering av en primer til nevnte prøvesekvens slik at basen i 3' enden av primeren blir hybridisert til den basen i prøvesekvensen som er rett ved siden av den spesifiserte posisjonen på 3' siden derav, (c) behandling av primeren under ekstensjonsbetingelser med en merket del som har evne til å tilveiebringe en ekstensjonsenhet av primeren i en posisjon som tilsvarer nevnte spesifiserte posisjon i prøvesekvensen dersom nevnte bestemte base er tilstede i den posisjonen, i det ekstensjonsbetingelsene er slik at en hvilke som helst ekstensjon av primeren ikke fortsetter utover nevnte spesifiserte posisjon av prøvesekvensen på annen måte enn ved ekstensjon med merket del, (d) vasking av bæreren for å fjerne ureagert merket del og andre reagenser, og (e) testing for innkorporering av merket del i primeren.
NO980837A 1995-09-02 1998-02-27 Identifisering av baser i nukleinsyresekvenser NO980837L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9517914.9A GB9517914D0 (en) 1995-09-02 1995-09-02 Identification of bases in nucleic acid sequences
PCT/GB1996/002136 WO1997009449A1 (en) 1995-09-02 1996-09-02 Identification of bases in nucleic acid sequences

Publications (2)

Publication Number Publication Date
NO980837D0 NO980837D0 (no) 1998-02-27
NO980837L true NO980837L (no) 1998-04-30

Family

ID=10780089

Family Applications (1)

Application Number Title Priority Date Filing Date
NO980837A NO980837L (no) 1995-09-02 1998-02-27 Identifisering av baser i nukleinsyresekvenser

Country Status (8)

Country Link
EP (1) EP0850317A1 (no)
JP (1) JPH11511981A (no)
AU (1) AU6833996A (no)
CA (1) CA2230893A1 (no)
GB (1) GB9517914D0 (no)
IL (1) IL123513A0 (no)
NO (1) NO980837L (no)
WO (1) WO1997009449A1 (no)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6146828A (en) * 1996-08-14 2000-11-14 Exact Laboratories, Inc. Methods for detecting differences in RNA expression levels and uses therefor
US6566101B1 (en) * 1997-06-16 2003-05-20 Anthony P. Shuber Primer extension methods for detecting nucleic acids
US6326489B1 (en) 1997-08-05 2001-12-04 Howard Hughes Medical Institute Surface-bound, bimolecular, double-stranded DNA arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
EP1028970A1 (en) 1997-10-10 2000-08-23 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6280947B1 (en) 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE66572B1 (en) * 1989-02-13 1996-01-24 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
IL97222A (en) * 1990-02-16 1995-08-31 Orion Yhtymae Oy Method and Responder for Determining Special Changes to Nucleotide
WO1992016657A1 (en) * 1991-03-13 1992-10-01 E.I. Du Pont De Nemours And Company Method of identifying a nucleotide present at a defined position in a nucleic acid
RU2186852C2 (ru) * 1991-12-24 2002-08-10 Тепнел Медикал Лимитед Способ выполнения манипуляции с последовательностью нуклеиновой кислоты, устройство для проведения манипуляций на последовательности нуклеиновой кислоты, проточный сосуд, твердый носитель для иммобилизации последовательности нуклеиновой кислоты, твердый носитель, способ получения носителя для иммобилизации последовательности нуклеиновой кислоты
GB9210176D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
GB2283815B (en) * 1993-11-04 1997-11-19 Tepnel Medical Ltd Capture of a nucleic acid sequence by hybridisation to an oligonucleotide bonded to a solid support

Also Published As

Publication number Publication date
WO1997009449A1 (en) 1997-03-13
IL123513A0 (en) 1998-10-30
CA2230893A1 (en) 1997-03-13
EP0850317A1 (en) 1998-07-01
GB9517914D0 (en) 1995-11-01
AU6833996A (en) 1997-03-27
JPH11511981A (ja) 1999-10-19
NO980837D0 (no) 1998-02-27

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