NO975689L - Antineutrophil cytoplasmic antibody material associated with ulcerative colitis and related methods and assays - Google Patents

Antineutrophil cytoplasmic antibody material associated with ulcerative colitis and related methods and assays

Info

Publication number
NO975689L
NO975689L NO975689A NO975689A NO975689L NO 975689 L NO975689 L NO 975689L NO 975689 A NO975689 A NO 975689A NO 975689 A NO975689 A NO 975689A NO 975689 L NO975689 L NO 975689L
Authority
NO
Norway
Prior art keywords
ucpanca
libraries
methods
assays
ulcerative colitis
Prior art date
Application number
NO975689A
Other languages
Norwegian (no)
Other versions
NO975689D0 (en
Inventor
Jonathan Braun
Mark P Eggena
Stephan R Targan
Original Assignee
Cedars Sinai Medical Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cedars Sinai Medical Center filed Critical Cedars Sinai Medical Center
Publication of NO975689D0 publication Critical patent/NO975689D0/en
Publication of NO975689L publication Critical patent/NO975689L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/686—Anti-idiotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Foreliggende oppfinnelse til- veiebringer for første gang isolert, i det vesentlige rent og/eller rekombin- ant fremstilt pANCA forbundet med ul- cerøs kolitt (UCpANCA og UCpANCA- materiale) så vel som polynukleotider som koder for UCpANCA, UCpANCA-materi- ale og UCpANCA-polypeptider ifølge foreliggende oppfinnelse. Ved å benytte fagfremvis- ningsteknikken har immunoglobulingen- repertoarer anriket på immunoglobulin- gener som benyttes av pANCA-seroposi- tive UC-pasienter, blitt isolert, amplifisert, tilfeldig rekombinert for dannelse av fagmidekspresjonsvektor- biblioteker som koder for DNA-homologer fra genrepertoaret. Fremgangsmåter til- veiebringes for anrikning av disse biblioteker slik at det dannes biblio- teker av VH- og VL-kodende DNA-homologer med immunoreaktivitet overfor UCpANCA- antigen. Videre tilveiebringes frem- gangsmåter for gjennomsøking av biblio- tekene ifølge foreliggende oppfinnelse for UCpANCA og UCpANCA-materiale. Disse biblioteker kan innkapsles i fagpartik- ler eller i celler. Fremgangsmåter og analysesett tilveiebringes også for analyse av UCpANCA i en prøve og isolering av UCpANCA-antigen.The present invention provides for the first time isolated, substantially pure and / or recombinantly produced pANCA associated with ulcerative colitis (UCpANCA and UCpANCA material) as well as polynucleotides encoding UCpANCA, UCpANCA material and UCpANCA polypeptides of the present invention. Using the phage display technique, immunoglobulin gene repertories enriched with immunoglobulin genes used by pANCA seropositive UC patients have been isolated, amplified, randomly recombined to form phagemid expression vector libraries encoding DNA homologs from gene homologs. Methods are provided for enriching these libraries to form libraries of VH and VL coding DNA homologs with immunoreactivity to UCpANCA antigen. Further, methods for scanning the libraries of the present invention for UCpANCA and UCpANCA material are provided. These libraries can be encapsulated in phage particles or cells. Methods and assays are also provided for analysis of UCpANCA in a sample and isolation of UCpANCA antigen.

NO975689A 1995-06-06 1997-12-05 Antineutrophil cytoplasmic antibody material associated with ulcerative colitis and related methods and assays NO975689L (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47268895A 1995-06-06 1995-06-06
PCT/US1996/008756 WO1996039186A1 (en) 1995-06-06 1996-06-05 Anti-neutrophil cytoplasmic antibody material associated with ulcerative colitis and related methods and kits

Publications (2)

Publication Number Publication Date
NO975689D0 NO975689D0 (en) 1997-12-05
NO975689L true NO975689L (en) 1998-02-05

Family

ID=23876540

Family Applications (1)

Application Number Title Priority Date Filing Date
NO975689A NO975689L (en) 1995-06-06 1997-12-05 Antineutrophil cytoplasmic antibody material associated with ulcerative colitis and related methods and assays

Country Status (7)

Country Link
EP (1) EP0837697A1 (en)
JP (1) JPH11507516A (en)
AU (1) AU6040396A (en)
CA (1) CA2223439A1 (en)
IL (1) IL122481A0 (en)
NO (1) NO975689L (en)
WO (1) WO1996039186A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2324514A1 (en) * 1998-04-03 1999-10-14 Invitrogen Corporation Methods for producing libraries of expressible gene sequences
GB0605965D0 (en) * 2006-03-24 2006-05-03 Univ East Anglia Fluorescence based detection of substances

Also Published As

Publication number Publication date
NO975689D0 (en) 1997-12-05
MX9709504A (en) 1998-10-31
IL122481A0 (en) 1998-06-15
EP0837697A1 (en) 1998-04-29
JPH11507516A (en) 1999-07-06
WO1996039186A1 (en) 1996-12-12
CA2223439A1 (en) 1996-12-12
AU6040396A (en) 1996-12-24

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