NO970609L - Improved purification composition - Google Patents

Improved purification composition

Info

Publication number
NO970609L
NO970609L NO970609A NO970609A NO970609L NO 970609 L NO970609 L NO 970609L NO 970609 A NO970609 A NO 970609A NO 970609 A NO970609 A NO 970609A NO 970609 L NO970609 L NO 970609L
Authority
NO
Norway
Prior art keywords
alpha
amylases
mutant
precursor
amylase
Prior art date
Application number
NO970609A
Other languages
Norwegian (no)
Other versions
NO970609D0 (en
Inventor
Christopher C Barnett
Colin Mitchinson
Scott D Power
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Publication of NO970609D0 publication Critical patent/NO970609D0/en
Publication of NO970609L publication Critical patent/NO970609L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/39—Organic or inorganic per-compounds
    • C11D3/3942—Inorganic per-compounds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/39—Organic or inorganic per-compounds
    • C11D3/3947—Liquid compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Abstract

Nye alfa-amylase mutanter avledet fra DNA sekvensene til naturlig forekommende eller rekombinante alfa-amylaser er beskrevet. Mutante alfa-amylaser blir oppnådd ved in vitro modifikasjoner av en forløper DNA sekvens kodende for nahirlig forekommende eller rekombinant alfa-amylase for å danne substitusjon (erstatning) eller delesjon av en eller flere oksiderbare aminosyreresidier i aminosyresekvensen til en forløper alfa- amylase. Slike mutante alfa-amylaser har endret oksidativ stabilitet og/eller endret pH ytelsesprofiler og/eller endret termisk stabilitet sammenlignet med forløperen. Detergent og stivelsesflytendegjorende sammensetninger omfattende mutante amylaser, samt fremgangsmåter for anvendelse av slike mutante amylaser. Spesielt foretrukket er mutante alfa-amylaser fra Bacillus licheniformis modifisert ved MET197 eller MET15 eller ved TRP138 residiene eller ved ekvivalente residier til alle alfa-amylaser fra andre mikrobielle kilder (Bacillus, Aspergillus).New alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are described. Mutant alpha-amylases are obtained by in vitro modifications of a precursor DNA sequence encoding hardly present or recombinant alpha-amylase to form substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and / or altered pH performance profiles and / or altered thermal stability compared to the precursor. Detergent and starch liquid compositions comprising mutant amylases, and methods for using such mutant amylases. Particularly preferred are mutant alpha-amylases from Bacillus licheniformis modified by the MET197 or MET15 or by the TRP138 residues or by equivalent residues to all alpha-amylases from other microbial sources (Bacillus, Aspergillus).

NO970609A 1994-08-11 1997-02-10 Improved purification composition NO970609L (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28935194A 1994-08-11 1994-08-11
PCT/US1995/010426 WO1996005295A2 (en) 1994-08-11 1995-08-09 An improved cleaning composition

Publications (2)

Publication Number Publication Date
NO970609D0 NO970609D0 (en) 1997-02-10
NO970609L true NO970609L (en) 1997-03-24

Family

ID=23111158

Family Applications (1)

Application Number Title Priority Date Filing Date
NO970609A NO970609L (en) 1994-08-11 1997-02-10 Improved purification composition

Country Status (15)

Country Link
EP (1) EP0775201A2 (en)
JP (1) JPH10504197A (en)
KR (1) KR970704872A (en)
CN (1) CN1158637A (en)
AU (1) AU686007B2 (en)
BR (1) BR9508582A (en)
CA (1) CA2197203A1 (en)
CO (1) CO4440440A1 (en)
FI (1) FI970563A (en)
HU (1) HUT77748A (en)
MX (1) MX9700776A (en)
NO (1) NO970609L (en)
NZ (1) NZ291984A (en)
PL (1) PL318209A1 (en)
WO (1) WO1996005295A2 (en)

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Also Published As

Publication number Publication date
WO1996005295A2 (en) 1996-02-22
HUT77748A (en) 1998-07-28
BR9508582A (en) 1998-06-02
CO4440440A1 (en) 1997-05-07
FI970563A0 (en) 1997-02-10
NO970609D0 (en) 1997-02-10
NZ291984A (en) 1998-04-27
KR970704872A (en) 1997-09-06
CA2197203A1 (en) 1996-02-22
EP0775201A2 (en) 1997-05-28
AU3366295A (en) 1996-03-07
CN1158637A (en) 1997-09-03
JPH10504197A (en) 1998-04-28
AU686007B2 (en) 1998-01-29
MX9700776A (en) 1997-05-31
PL318209A1 (en) 1997-05-26
WO1996005295A3 (en) 1996-03-28
FI970563A (en) 1997-02-10

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