NO895029L - CLONING AND EXPRESSION OF TRANSFORMING GROWTH FACTOR BETA2 - Google Patents

CLONING AND EXPRESSION OF TRANSFORMING GROWTH FACTOR BETA2

Info

Publication number
NO895029L
NO895029L NO89895029A NO895029A NO895029L NO 895029 L NO895029 L NO 895029L NO 89895029 A NO89895029 A NO 89895029A NO 895029 A NO895029 A NO 895029A NO 895029 L NO895029 L NO 895029L
Authority
NO
Norway
Prior art keywords
tgf
expression
transfected
cloning
cells
Prior art date
Application number
NO89895029A
Other languages
Norwegian (no)
Other versions
NO895029D0 (en
Inventor
Anthony F Purchio
Linda Madisen
Nancy Webb
Original Assignee
Oncogen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncogen filed Critical Oncogen
Publication of NO895029D0 publication Critical patent/NO895029D0/en
Publication of NO895029L publication Critical patent/NO895029L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/495—Transforming growth factor [TGF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Toxicology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

cDNA kloner kodende for TGF-P2 som blir anvendt for å konstruere ekspresjonsvektorer som kan lede ekspresjon av moden, biologisk aktiv TGF-32 samt forløper TGF-P2 former l transfekterte kinesiske hamster-ovarie celler ! (CHO-celler) l høyt nivå og transfekterte COS-celler er beskrevet. CHO og COS transfektanter som utskiller TGF-02 l høye nivåer er også beskrevet. CHO-celler transfektert med en plasmldvektor Inneholdende hele 414 l aminosyre slmlan TGF-B2 forløperen utskiller omtrent 5ug pr. ml kulturmedlum.cDNA clones encoding TGF-P2 used to construct expression vectors capable of directing expression of mature, biologically active TGF-32 as well as TGF-P2 progenitor forms in transfected Chinese hamster ovary cells! (CHO cells) at high level and transfected COS cells are described. CHO and COS transfectants that secrete high levels of TGF-02 are also described. CHO cells transfected with a plasmid vector ml of culture media.

NO89895029A 1988-12-16 1989-12-14 CLONING AND EXPRESSION OF TRANSFORMING GROWTH FACTOR BETA2 NO895029L (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US28514088A 1988-12-16 1988-12-16

Publications (2)

Publication Number Publication Date
NO895029D0 NO895029D0 (en) 1989-12-14
NO895029L true NO895029L (en) 1990-06-18

Family

ID=23092909

Family Applications (1)

Application Number Title Priority Date Filing Date
NO89895029A NO895029L (en) 1988-12-16 1989-12-14 CLONING AND EXPRESSION OF TRANSFORMING GROWTH FACTOR BETA2

Country Status (5)

Country Link
JP (1) JPH0556783A (en)
KR (1) KR900009984A (en)
CA (1) CA2005459A1 (en)
NO (1) NO895029L (en)
ZA (1) ZA899623B (en)

Also Published As

Publication number Publication date
JPH0556783A (en) 1993-03-09
NO895029D0 (en) 1989-12-14
KR900009984A (en) 1990-07-06
ZA899623B (en) 1990-09-26
CA2005459A1 (en) 1990-06-16

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