NO791493L - KINETICAL MEASUREMENT OF GLUCOSE CONCENTRATION IN BODY LIQUIDS AND FORMULATIONS FOR USE THEREOF - Google Patents
KINETICAL MEASUREMENT OF GLUCOSE CONCENTRATION IN BODY LIQUIDS AND FORMULATIONS FOR USE THEREOFInfo
- Publication number
- NO791493L NO791493L NO791493A NO791493A NO791493L NO 791493 L NO791493 L NO 791493L NO 791493 A NO791493 A NO 791493A NO 791493 A NO791493 A NO 791493A NO 791493 L NO791493 L NO 791493L
- Authority
- NO
- Norway
- Prior art keywords
- reagent
- glucose concentration
- mannose
- glucose
- formulations
- Prior art date
Links
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims description 23
- 239000008103 glucose Substances 0.000 title claims description 23
- 239000000203 mixture Substances 0.000 title claims description 13
- 239000007788 liquid Substances 0.000 title claims description 6
- 238000005259 measurement Methods 0.000 title description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 15
- 238000009472 formulation Methods 0.000 claims description 12
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 7
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims description 7
- 102000005548 Hexokinase Human genes 0.000 claims description 6
- 108700040460 Hexokinases Proteins 0.000 claims description 6
- 238000010521 absorption reaction Methods 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 238000006911 enzymatic reaction Methods 0.000 claims description 2
- 238000010998 test method Methods 0.000 claims description 2
- 238000012956 testing procedure Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 12
- 239000003708 ampul Substances 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 4
- 210000001124 body fluid Anatomy 0.000 description 4
- 239000010839 body fluid Substances 0.000 description 4
- 102100031126 6-phosphogluconolactonase Human genes 0.000 description 3
- 108010029731 6-phosphogluconolactonase Proteins 0.000 description 3
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 3
- 239000011230 binding agent Substances 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- NBSCHQHZLSJFNQ-QTVWNMPRSA-N D-Mannose-6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@@H]1O NBSCHQHZLSJFNQ-QTVWNMPRSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 241000978776 Senegalia senegal Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000007824 enzymatic assay Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000012933 kinetic analysis Methods 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 229940069446 magnesium acetate Drugs 0.000 description 1
- 235000011285 magnesium acetate Nutrition 0.000 description 1
- 239000011654 magnesium acetate Substances 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000010356 sorbitol Nutrition 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- -1 triethanomamine Chemical compound 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Emergency Medicine (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
Foreliggende oppfinnelse angår en ny metode og formuler-ing for kinetisk bestemmelse av glucosekonsentrasjon i kroppsvæsker ved anvendelse av hexokinasekoblede enzymprøvningsmetode. The present invention relates to a new method and formulation for the kinetic determination of glucose concentration in body fluids using hexokinase-coupled enzyme testing methods.
Den kjente metode for kobling av enzymene hexokinase og glucose-6-fosfatdehydrogenase gir et meget spesifikt system for bestemmelse av glucose i kroppsvæsker, slik som serum, ved fullsten-dig omsetning av prøven inneholdende glucose efter følgende enzy-matiske trinn: The known method for coupling the enzymes hexokinase and glucose-6-phosphate dehydrogenase provides a very specific system for the determination of glucose in body fluids, such as serum, by complete conversion of the sample containing glucose following the following enzymatic steps:
Dannelsen av NADH målt spektrofotometrisk ved 340 nm er direkte proporsjonal med glucosekonsentrasjonen i væskeprøven så-snart all glucosen er blitt omsatt. The formation of NADH measured spectrophotometrically at 340 nm is directly proportional to the glucose concentration in the liquid sample as soon as all the glucose has been converted.
Det er imidlertid erkjent at der foreligger behov for en prøvningsprosedyre som eliminerer behovet for fullføring av enzym-koblingsreaksjonen, og som dermed vil føre til større prøveantall/ tidsenhet. Det er også erkjent ønskelig å fjerne behovet for serum, blindprøveabsorpsjonskorreksjoner som er kostbare og tidkrev-ende. Videre ville en kinetisk reaksjonshastighet-prøvningsprose-dyre generelt være mere spesifikk og ville også lettere kunne au-tomatiseres . However, it is recognized that there is a need for a test procedure which eliminates the need for completion of the enzyme-coupling reaction, and which will thus lead to a larger number of samples/unit of time. It is also recognized as desirable to remove the need for serum, blank sample absorption corrections which are expensive and time-consuming. Furthermore, a kinetic reaction rate testing procedure would generally be more specific and would also be more easily automated.
Det er derfor blitt foreslått å anvende en kinetisk bun-det tidsprøvning for glucose under anvendelse av hexokinase/gluco-■ se-6-fosfatdehydrogenase reagenssystem under anvendelse av N-acet-yl -glucosamin som en valgt reaksjonskinetisk inhibitor. It has therefore been proposed to use a kinetic time trial for glucose using the hexokinase/glucose-6-phosphate dehydrogenase reagent system using N-acetyl-glucosamine as a selected reaction kinetic inhibitor.
Det er nu funnet at en egnet koblet enzymatisk prøvnings-prosedyre under anvendelse av hexokinase/glucose-6---f osf atdehydroge-nasereagenssystem kan anvendes for kinetisk måling av glucosekonsentrasjonen i en kroppsvæske hvor hastigheten av NADH-dannelsen er proporsjonal med hastigheten av glucose som reagerer, som i sin tur er proporsjonal med glucosekonsentrasjonen i kroppsvæskeprøven, It has now been found that a suitable coupled enzymatic assay procedure using the hexokinase/glucose-6-phosphate dehydrogenase reagent system can be used for the kinetic measurement of the glucose concentration in a body fluid where the rate of NADH formation is proportional to the rate of glucose which reacts, which in turn is proportional to the glucose concentration in the body fluid sample,
En slik forbedret koblet enzymprøvningsprosedyre er blitt muliggjort ved at det er funnet at tilsetning av D-mannose påvirker de kinetiske egenskaper til hexokinase^-enzymreaks jonstrinnet. D-mannose omdannes til mannose-6-fosfat in situ, som virker som en produktinhibitor for hexokinase og derved forårsaker at hastigheten av NADH-dannelsen er proporsjonal med glucosekonsentrasjonen i prø-ven . Such an improved coupled enzyme assay procedure has been made possible by the finding that the addition of D-mannose affects the kinetic properties of the hexokinase^ enzyme reaction step. D-mannose is converted to mannose-6-phosphate in situ, which acts as a product inhibitor for hexokinase and thereby causes the rate of NADH formation to be proportional to the glucose concentration in the sample.
Det resulterende system utviser lineær kinetikk ved ca. 30°C i minst 1 til mer enn 5 minutter, avhengig av glucosekonsen-tras jonen. The resulting system exhibits linear kinetics at approx. 30°C for at least 1 to more than 5 minutes, depending on the glucose concentration.
Det er generelt funnet at innbefattelse av ca. 4 g D-mannose pr. liter reagens bevirker detønskede resultat, nemlig å tilveiebringe et system hvorved hastigheten av NADH-dannelsen er proporsjonal med hastigheten av glucosekonsentrasjonen i væskeprø- . ven. Et slikt kinetisk system for glucosebestemmelsen er meget .fordelaktig når det gjelder nøyaktighet og presisjon i forhold til den tidligere anvendte glucose-endepunktmetode. Således er dette kinetiske system egnet for bruk med enhver sentrifugal eller hur-tig kinetisk analyseringsanordning slik som f.eks. Gilford 2000 eller 35000 modellene, Perkin-Elmer KA-150 og andre analyserings-anordninger. It is generally found that inclusion of approx. 4 g D-mannose per liter of reagent produces the desired result, namely to provide a system whereby the rate of NADH formation is proportional to the rate of glucose concentration in the liquid sample. friend. Such a kinetic system for the glucose determination is very advantageous in terms of accuracy and precision compared to the previously used glucose endpoint method. Thus, this kinetic system is suitable for use with any centrifugal or fast kinetic analysis device such as e.g. Gilford 2000 or 35000 models, Perkin-Elmer KA-150 and other analyzing devices.
Det kinetiske reaksjonssystem ifølge oppfinnelsen gir en lineær kalibreringskurve over glucosekonsentrasjonsområdet på fra 50 til minst 750 mg/dl og er lineær i et tidsrom på opp til minst The kinetic reaction system according to the invention provides a linear calibration curve over the glucose concentration range of from 50 to at least 750 mg/dl and is linear for a time period of up to at least
5 minutter over et temperaturområde fra 23 til 37°C. 5 minutes over a temperature range from 23 to 37°C.
Hastigheten med hvilken glucose omsettes, bestemmes ved måling av absorpsjonen av NADH spektrofotometrisk ved ca. 334 til 366 nm, fortrinnsvis ved 340 nm. The rate at which glucose is converted is determined by measuring the absorption of NADH spectrophotometrically at approx. 334 to 366 nm, preferably at 340 nm.
Reagensformuleringer for utførelse av en kinetisk reak-sjonsmåling av glucosekonsentrasjonen ifølge oppfinnelsen og avhengig av arten av den kliniske instrumentering for hvilken rea- genset skal anvendes, kan formuleres ved innbefattelse av D-mannose i enten en enkel ampulleformulering hvori. alle. de nødvendige, bestanddeler for testreaksjonen er innbefattet i en prø<y>ebeholder eller i en dobbelt ampulleformulering hvori én ampulle inneholder et "substrat" inneholdende alle reaktanter unntatt én, og en annen ampulle inneholdende "triggeroppløsningen". Reagent formulations for carrying out a kinetic reaction measurement of the glucose concentration according to the invention and depending on the nature of the clinical instrumentation for which the reagent is to be used, can be formulated by including D-mannose in either a simple ampoule formulation in which. everyone. the necessary components for the test reaction are contained in a sample container or in a double ampoule formulation in which one ampoule contains a "substrate" containing all reactants except one, and another ampoule containing the "trigger solution".
Typiske eksempler på slike formuleringer og deres frem-stilling er angitt i de efterfølgende eksempler: Typical examples of such formulations and their preparation are indicated in the following examples:
EKSEMPEL II DOBBEL AMPULLEFORMULERING EXAMPLE II DUAL AMPOULE FORMULATION
Det skal forståes at de ovenfor angitte formuleringer bare er angitt som eksempler og ikke er begrenset til disse. Ek-sempelvis kan andre egnede bestanddeler anvendes. I stedet for dextran-bindemiddel kan i stedet anvendes andre bindemidler slik som gummi arabicum, mannitol, sorbitol eller lignende. Som andre egnede proteinkilder i stedet for kvegserumalbumin kan anvendes globuliner, gelatiner eller lignende. Andre egnede buffere innbefatter f.eks. tris(hydroxymethyl)aminomethan, triethanomamin, N-2-hydroxyethylpiperazin-N'-2-ethansulfonsyre og lignende. Andre egnede magnesiumsalter vil innbefatte magnesiumacetat, nit-rat, sulfat og lignende. It should be understood that the formulations given above are only given as examples and are not limited thereto. For example, other suitable components can be used. Instead of dextran binder, other binders such as gum arabic, mannitol, sorbitol or the like can be used instead. As other suitable protein sources instead of bovine serum albumin, globulins, gelatins or the like can be used. Other suitable buffers include e.g. tris(hydroxymethyl)aminomethane, triethanomamine, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid and the like. Other suitable magnesium salts will include magnesium acetate, nitrate, sulfate and the like.
Nytten av formuleringene ifølge oppfinnelsen og lineari-teten av den kinetiske reaksjonshastighet av forandringen i ab-^ sorpsjon av NADH er vist i de efterfølgende eksempler hvori den enkle ampulleformulering ifølge eksempel I ble anvendt for å måle forandringen i absorpsjon ved 340 nm ved 30°C for standardprøver med kjent glucosekonsentrasjon . Forandringen i absorpsjon ble bestemt under anvendelse av et Gilford 2000 spektrofotometer. The usefulness of the formulations according to the invention and the linearity of the kinetic reaction rate of the change in absorption of NADH is shown in the following examples in which the simple ampoule formulation according to Example I was used to measure the change in absorption at 340 nm at 30°C for standard samples with known glucose concentration. The change in absorbance was determined using a Gilford 2000 spectrophotometer.
EKSEMPEL III EXAMPLE III
Prøvevolum, 2,0 ml reagens +10 L prøve Sample volume, 2.0 ml reagent +10 L sample
EKSEMPEL IV EXAMPLE IV
Prøvevolum, 2,0 ml reagens + 20 L prøve Sample volume, 2.0 ml reagent + 20 L sample
EKSEMPEL V EXAMPLE V
Prøvevolum, 2,0 ml reagems +25 L prøve Sample volume, 2.0 ml reagent + 25 L sample
Claims (5)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US90310978A | 1978-05-05 | 1978-05-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO791493L true NO791493L (en) | 1979-11-06 |
Family
ID=25416950
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO791493A NO791493L (en) | 1978-05-05 | 1979-05-04 | KINETICAL MEASUREMENT OF GLUCOSE CONCENTRATION IN BODY LIQUIDS AND FORMULATIONS FOR USE THEREOF |
Country Status (10)
| Country | Link |
|---|---|
| JP (1) | JPS54147098A (en) |
| BE (1) | BE875965A (en) |
| DE (1) | DE2917443A1 (en) |
| DK (1) | DK185679A (en) |
| FR (1) | FR2425071A1 (en) |
| GB (1) | GB2020424B (en) |
| IT (1) | IT1116032B (en) |
| NL (1) | NL7903113A (en) |
| NO (1) | NO791493L (en) |
| SE (1) | SE7903821L (en) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI81120C (en) * | 1988-09-26 | 1990-09-10 | Kone Oy | FOERFARANDE FOER BESTAEMNING AV GLUKOS UR BIOLOGISKA VAETSKA SAMT REAGENSBLANDNING FOER TILLAEMPNING AV FOERFARANDET. |
| AUPM506894A0 (en) * | 1994-04-14 | 1994-05-05 | Memtec Limited | Novel electrochemical cells |
| AUPN239395A0 (en) * | 1995-04-12 | 1995-05-11 | Memtec Limited | Method of defining an electrode area |
| US6413410B1 (en) | 1996-06-19 | 2002-07-02 | Lifescan, Inc. | Electrochemical cell |
| AUPN363995A0 (en) | 1995-06-19 | 1995-07-13 | Memtec Limited | Electrochemical cell |
| US6521110B1 (en) * | 1995-11-16 | 2003-02-18 | Lifescan, Inc. | Electrochemical cell |
| US6638415B1 (en) | 1995-11-16 | 2003-10-28 | Lifescan, Inc. | Antioxidant sensor |
| US6863801B2 (en) | 1995-11-16 | 2005-03-08 | Lifescan, Inc. | Electrochemical cell |
| AUPN661995A0 (en) * | 1995-11-16 | 1995-12-07 | Memtec America Corporation | Electrochemical cell 2 |
| IL156007A0 (en) | 2001-10-10 | 2003-12-23 | Lifescan Inc | Electrochemical cell |
| US8529751B2 (en) | 2006-03-31 | 2013-09-10 | Lifescan, Inc. | Systems and methods for discriminating control solution from a physiological sample |
| US8778168B2 (en) | 2007-09-28 | 2014-07-15 | Lifescan, Inc. | Systems and methods of discriminating control solution from a physiological sample |
| US8603768B2 (en) | 2008-01-17 | 2013-12-10 | Lifescan, Inc. | System and method for measuring an analyte in a sample |
| US8551320B2 (en) | 2008-06-09 | 2013-10-08 | Lifescan, Inc. | System and method for measuring an analyte in a sample |
| US10317359B2 (en) | 2016-01-05 | 2019-06-11 | Ravi Kumar Meruva | Differential carbon dioxide sensor |
-
1979
- 1979-04-20 NL NL7903113A patent/NL7903113A/en not_active Application Discontinuation
- 1979-04-24 IT IT48832/79A patent/IT1116032B/en active
- 1979-04-26 GB GB7914580A patent/GB2020424B/en not_active Expired
- 1979-04-28 DE DE19792917443 patent/DE2917443A1/en not_active Withdrawn
- 1979-04-30 BE BE0/194926A patent/BE875965A/en unknown
- 1979-05-02 JP JP5356979A patent/JPS54147098A/en active Pending
- 1979-05-02 SE SE7903821A patent/SE7903821L/en not_active Application Discontinuation
- 1979-05-04 DK DK185679A patent/DK185679A/en not_active Application Discontinuation
- 1979-05-04 FR FR7911330A patent/FR2425071A1/en active Pending
- 1979-05-04 NO NO791493A patent/NO791493L/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| GB2020424A (en) | 1979-11-14 |
| FR2425071A1 (en) | 1979-11-30 |
| SE7903821L (en) | 1979-11-06 |
| GB2020424B (en) | 1982-08-25 |
| IT7948832A0 (en) | 1979-04-24 |
| BE875965A (en) | 1979-08-16 |
| IT1116032B (en) | 1986-02-10 |
| JPS54147098A (en) | 1979-11-16 |
| DK185679A (en) | 1979-11-06 |
| DE2917443A1 (en) | 1979-11-08 |
| NL7903113A (en) | 1979-11-07 |
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