NO20015181L - Expression of heparin-binding protein in recombinant mammalian cells - Google Patents

Expression of heparin-binding protein in recombinant mammalian cells

Info

Publication number
NO20015181L
NO20015181L NO20015181A NO20015181A NO20015181L NO 20015181 L NO20015181 L NO 20015181L NO 20015181 A NO20015181 A NO 20015181A NO 20015181 A NO20015181 A NO 20015181A NO 20015181 L NO20015181 L NO 20015181L
Authority
NO
Norway
Prior art keywords
binding protein
expression
cell
heparin
mammalian cells
Prior art date
Application number
NO20015181A
Other languages
Norwegian (no)
Other versions
NO20015181D0 (en
Inventor
Hans Jakob Flodgaard
Poul Baad Rasmussen
Soeren Bjoern
Ivan Svendsen
Original Assignee
Leukotech As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leukotech As filed Critical Leukotech As
Publication of NO20015181D0 publication Critical patent/NO20015181D0/en
Publication of NO20015181L publication Critical patent/NO20015181L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4723—Cationic antimicrobial peptides, e.g. defensins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Foreliggende oppfinnelse omhandler en fremgangsmåte for å fremstille et pattedyr heparinbindende protein i en pattedyrcelle som kan bli dyrket under anaerobe betingelser etter å ha introdusert en nukleinsyre som koder som heparinbindende protein inn i nevnte celle som omfatter (a) å introdusere inn i nevnte pattedyrcelle en nukleinsyre som koder for nevnte heparinbindende protein; (b) å dyrke cellen fra trinn (a) under betingelser som er fremmende for ekspresjon av nevnte HBP; og (c) å gjenvinne nevnte HBP fra dyrkningsmediet. Videre omhandler oppfinnelsen den rekombinante pattedyrcellen som blir benyttet i fremgangsmåten.The present invention relates to a process for producing a mammalian heparin binding protein in a mammalian cell which can be cultured under anaerobic conditions after introducing a nucleic acid encoding as heparin binding protein into said cell which comprises (a) introducing into said mammalian cell a nucleic acid cell encoding said heparin binding protein; (b) culturing the cell of step (a) under conditions conducive to expression of said HBP; and (c) recovering said HBP from the culture medium. Furthermore, the invention relates to the recombinant mammalian cell used in the method.

NO20015181A 1999-04-29 2001-10-23 Expression of heparin-binding protein in recombinant mammalian cells NO20015181L (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13157499P 1999-04-29 1999-04-29
DKPA199900612 1999-05-06
PCT/DK2000/000216 WO2000066627A1 (en) 1999-04-29 2000-04-28 Expression of heparin-binding protein in recombinant mammalian cells

Publications (2)

Publication Number Publication Date
NO20015181D0 NO20015181D0 (en) 2001-10-23
NO20015181L true NO20015181L (en) 2001-12-20

Family

ID=26064312

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20015181A NO20015181L (en) 1999-04-29 2001-10-23 Expression of heparin-binding protein in recombinant mammalian cells

Country Status (13)

Country Link
EP (1) EP1179017A1 (en)
JP (1) JP2002542813A (en)
KR (1) KR20020034073A (en)
CN (1) CN1357007A (en)
AU (1) AU4286100A (en)
CA (1) CA2407714A1 (en)
EA (1) EA200101093A1 (en)
HK (1) HK1046005A1 (en)
IL (1) IL146128A0 (en)
NO (1) NO20015181L (en)
NZ (1) NZ515762A (en)
PL (1) PL351499A1 (en)
WO (1) WO2000066627A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003226907A1 (en) * 2002-03-27 2003-10-08 Leukotech A/S Method for the preparation of recombinant mammalian heparin-binding protein (hbp)
EP1739179A1 (en) * 2005-06-30 2007-01-03 Octapharma AG Serum-free stable transfection and production of recombinant human proteins in human cell lines
GB201102108D0 (en) * 2011-02-07 2011-03-23 Hansa Medical Ab Diagnostic method
CN110643586B (en) * 2019-10-15 2022-06-10 山东大学 Heparin skeleton synthase and coding gene and application thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0409858B1 (en) * 1988-03-17 1993-12-08 Novo Nordisk A/S Heparin-binding proteins, dna coding for them, processes for producing them as well as therapeutic preparations containing them
DE69530689T2 (en) * 1994-04-21 2004-03-25 Leukotech A/S HEPARIN BINDING PROTEIN FOR TREATING SEPSIS
WO1999000417A1 (en) * 1997-06-25 1999-01-07 Novo Nordisk A/S Production of heparin-binding protein in mammalian cells

Also Published As

Publication number Publication date
HK1046005A1 (en) 2002-12-20
JP2002542813A (en) 2002-12-17
NZ515762A (en) 2002-11-26
CA2407714A1 (en) 2000-11-09
EP1179017A1 (en) 2002-02-13
NO20015181D0 (en) 2001-10-23
EA200101093A1 (en) 2002-04-25
WO2000066627A1 (en) 2000-11-09
PL351499A1 (en) 2003-04-22
CN1357007A (en) 2002-07-03
KR20020034073A (en) 2002-05-08
AU4286100A (en) 2000-11-17
IL146128A0 (en) 2002-07-25

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