NO138738B - PROCEDURE AND APPARATUS FOR MANAGING MILK SAMPLES FOR QUALITY CONTROL - Google Patents
PROCEDURE AND APPARATUS FOR MANAGING MILK SAMPLES FOR QUALITY CONTROL Download PDFInfo
- Publication number
- NO138738B NO138738B NO753636A NO753636A NO138738B NO 138738 B NO138738 B NO 138738B NO 753636 A NO753636 A NO 753636A NO 753636 A NO753636 A NO 753636A NO 138738 B NO138738 B NO 138738B
- Authority
- NO
- Norway
- Prior art keywords
- sample
- milk
- analysis
- catalase
- analyzer
- Prior art date
Links
- 239000008267 milk Substances 0.000 title claims description 38
- 210000004080 milk Anatomy 0.000 title claims description 38
- 235000013336 milk Nutrition 0.000 title claims description 38
- 238000000034 method Methods 0.000 title claims description 10
- 238000003908 quality control method Methods 0.000 title claims description 6
- 102000016938 Catalase Human genes 0.000 claims description 18
- 108010053835 Catalase Proteins 0.000 claims description 18
- 238000004458 analytical method Methods 0.000 claims description 18
- 238000005070 sampling Methods 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 3
- -1 peroxide compound Chemical class 0.000 claims description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 10
- 230000000694 effects Effects 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 210000001082 somatic cell Anatomy 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000004396 mastitis Diseases 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/04—Dairy products
Landscapes
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Dairy Products (AREA)
Description
Foreliggende oppfinnelse vedrører en fremgangsmåte og et appa- The present invention relates to a method and an appa-
rat for behandling av prøver for kvalitetskontroll av melk. council for processing samples for quality control of milk.
For en formålstjenelig kvalitetskontroll av melk kreves det For an expedient quality control of milk, it is required
at den gir beskjed om melkens velegnethet for foredlingsformål eller som industrimelk. that it provides information on the suitability of the milk for processing purposes or as industrial milk.
De bakterier som nedsetter kvaliteten av avkjblt melk er psykotrofer. Særmerke for disse bakterier er en katalaseaktivitet. De samme egenskaper er typisk for store mengder utskilte somatiske celler i melken, som folge av jurbetennelse (mastit). Således er graden av katalaseaktivitet i avkjolt melk belysende for melkens kvalitet. Katalaseaktiviteten kan lett bestemmes i melk ved en klassisk katalasebestemmelse, idet katalaseen-zymet frigjor oksygen fra melk tilsatt hydrogenperoksyd, idet utviklingen av oksygen tiltar med stigende enzyminnhold i melken. The bacteria that reduce the quality of chilled milk are psychrotrophs. A distinctive feature of these bacteria is a catalase activity. The same characteristics are typical for large amounts of secreted somatic cells in the milk, as a result of udder inflammation (mastitis). Thus, the degree of catalase activity in cooled milk is indicative of the milk's quality. The catalase activity can be easily determined in milk by a classic catalase determination, as the catalase enzyme releases oxygen from milk to which hydrogen peroxide has been added, as the development of oxygen increases with increasing enzyme content in the milk.
Ved et slikt kontrollsystem, ifolge hvilke melkeprbver samles By such a control system, according to which milk samples are collected
fra produsentene og transporteres til et laboratorium hvor selve kontrollen og analyse utfores, foreligger imidlertid den ulempe at melkens kvalitet forandrer seg under transporten, som avhengig av omstendighetene kan ta ganske lang tid. Som folge av dette kan melkeprovene på analysestedet utvise dårli-gere prøveresultater enn de som ville blitt erholdt for den ferske melk, og som folge herav forandres klassifiseringen av melken og produsentens erstatning i en for produsenten uheldig retning. from the producers and transported to a laboratory where the actual control and analysis is carried out, there is however the disadvantage that the quality of the milk changes during transport, which depending on the circumstances can take quite a long time. As a result of this, the milk samples at the analysis site may show worse test results than those that would have been obtained for the fresh milk, and as a result, the classification of the milk and the producer's compensation are changed in a direction unfavorable to the producer.
Foreliggende oppfinnelse har derfor til hensikt å tilveiebringe en fremgangsmåte for å behandle prøvene, slik at de ovenfor omtalede ulemper kan elimineres. The present invention therefore aims to provide a method for treating the samples, so that the disadvantages mentioned above can be eliminated.
Fremgangsmåten ifølge oppfinnelsen er særpreget ved at man an-vender en transportabel prøvebeholder som er forsynt med eller kan forsynes med en i og for seg kjent katalaseanalysator, og at melkeprøven med standardvolum blandes i forbindelse med prøvetaking før transport av prøven med en for utførelse av katalaseanalysen nødvendig' mengde av en peroksydforbindelse, slik at katalaseanalysen skjer i det minste delvis allerede under transport av prøven. The method according to the invention is characterized by the fact that a transportable sample container is used which is equipped with or can be equipped with a catalase analyzer known per se, and that the milk sample with a standard volume is mixed in connection with sampling before transporting the sample with a for carrying out the catalase analysis necessary amount of a peroxide compound, so that the catalase analysis takes place at least partially already during transport of the sample.
En for utførelse av fremgangsmåten ifølge oppfinnelsen passende anordning kjennetegnes ved at den omfatter en transportabel prøvebeholder. Den transportable <p>røvebeholderen utformes og dimensjoneres passende på en slik måte at det passer inn i en kassett sammen med analyseapparatet, som anvendes for den videre analyse av melken. Den kan med fordel være løsbart forbundet med en i og for seg kjent katalaseanalysator. Analyseapparatet kan .herved bestå enten av et væskefylt målekar forsynt med en gradering som angir melkens kvalitetsklasse, A suitable device for carrying out the method according to the invention is characterized by the fact that it comprises a transportable sample container. The transportable <p>robe container is designed and dimensioned appropriately in such a way that it fits into a cassette together with the analysis apparatus, which is used for the further analysis of the milk. It can advantageously be releasably connected to a known per se catalase analyzer. The analysis apparatus can therefore consist of either a liquid-filled measuring vessel provided with a graduation indicating the milk's quality class,
et gasstilførselsrør som fører til prøve- og målekaret og et til atmosfæren førende overstrømningsrør for målevæsken, eller en trykkmåler som er forsynt med en gradering for an-givelse av melkens kvalitet. a gas supply pipe leading to the sample and measuring vessel and an overflow pipe leading to the atmosphere for the measuring liquid, or a pressure gauge which is equipped with a graduation for indicating the quality of the milk.
Med anvendelse av fremgangsmåten ifølge oppfinnelsen oppnår man de fordeler at den anvendte anordning fungerer som en kombinert kvalitetskontrollanordning og transportabel prøvebehol-der, hvorved den også kan anvendes ved utførelse av den endelige analyse av melken. Man kan således i en og samme melkeprøve ved siden av kvaliteten også fastslå sammensetningen oq de somatiske celler, og ytterligere mister tidsfaktoren ved transporten av melken sin betydning, idet kvalitetsbestemmelsen på-begynnes direkte ved prøvetakingen og ytterligere virker rea-gensen for kvalitetsprøvningen som stabiliseringsmiddel for prøven. Stabiliseringsmidlet/reagensen utgjør heller intet økonomisk problem. I det tilfellet analyse av sammensetningen utføres uten anvendelse av reagenser, eksempelvis ved anvendelse av infrarbdspektroskopi, kan provene anvendes som for. Ytterligere behover provetagningen ikke være aseptisk. Anordningen ifolge oppfinnelsen og fremgangsmåten for utfbrelse av katalaseanalysen er ytterligere så enkel at melkeprodusenten selv kan kontrollere kvaliteten av sin melk ved hjelp av anordningen. By using the method according to the invention, one achieves the advantages that the device used functions as a combined quality control device and transportable sample container, whereby it can also be used when performing the final analysis of the milk. In one and the same milk sample, the composition and the somatic cells can thus be determined in addition to the quality, and furthermore the time factor during the transport of the milk loses its importance, since the quality determination begins directly at the sampling and the reagent for the quality test also acts as a stabilizing agent for the sample. The stabilizer/reagent also poses no economic problem. In the event that analysis of the composition is carried out without the use of reagents, for example when using infrared spectroscopy, the sample can be used as before. Furthermore, sampling does not need to be aseptic. The device according to the invention and the method for carrying out the catalase analysis are further so simple that the milk producer himself can control the quality of his milk with the aid of the device.
Oppfinnelsen skal i det fblgende beskrives nærmere under hen-visning til den vedlagte tegning som viser et utfbrelseseksempel av en for utfbrelse av fremgangsmåten ifolge oppfinnelsen egnet anordning. The invention shall be described in more detail in the following with reference to the attached drawing which shows an example of a device suitable for carrying out the method according to the invention.
På tegningen betegner A en kombinert transportabel prøve- og reak-sjonsbeholder med en munning B. Med C betegnes en kvalitets-prbvemåler forsynt med en målegradering, eksempelvis en fast-lagt klasseinndeling. Måleanordningen er i den nedre ende eksempelvis forsynt med en slip som passer tett i munnings-åpningen B og i den ovre ende kan den lukkes med en propp F. Provemåleren C er fylt med en væske, eksempelvis vann opp til In the drawing, A denotes a combined transportable sample and reaction container with a mouth B. C denotes a quality sample meter provided with a measurement grade, for example a defined class division. The measuring device is provided at the lower end, for example, with a slip that fits tightly in the mouth opening B and at the upper end it can be closed with a stopper F. The sample gauge C is filled with a liquid, for example water up to
den overste streken på graderingen. Måleanordningen C er ytterligere forsynt med et kommuniserende ror E mellom karet A's ovre del og til proveanordniroens ovre del for innfbring av den gass som utvikles under analysens forlbp, samt et overstrbmnings-rbr D som forer ut til atmosfæren, gjennom hvilket den av gas-sen fortrengte væske kan fores ut. the top line on the graduation. The measuring device C is further provided with a communicating tube E between the upper part of the vessel A and the upper part of the sample device for introducing the gas that develops during the course of the analysis, as well as an overflow tube D that leads out to the atmosphere, through which the gas Displaced fluid can be lined out.
Ved anvendelse innfores i karet A et standardvolum av velblan-det produsentmelk. I karet A er fortrinnsvis på forhånd tilsatt hydrogenperoksyd også i en standardmengde. Hydrogenperoksydet kan anvendes i form av en 3%-ig opplbsning eller eventuelt i fast form. Et passende forhold mellom melkeprbven og hydrogenperoksydet er eksempelvis 5:1 ved anvendelse av 3%-ig hydrogenperoksyd, idet det totale volum av melkeprbven og reagenset eksempelvis kan være 50 ml.. Karet A påsettes umiddelbart med provemåleren C. Det oksygentrykk som utvikles som en eventuell funksjon av katalaseaktiviteten utjevnes ved hjelp av roret E i prbvemålerén C under fortrengning av målevæsken gjennom roret D. Da reaksjonen avsluttes etter ca. 3 timer angir væskenivået i provemåleren C katalaseaktiviteten og dermed melkens kvalitet. When using, a standard volume of well-mixed producer's milk is introduced into vessel A. In the vessel A, hydrogen peroxide is preferably added in advance, also in a standard quantity. The hydrogen peroxide can be used in the form of a 3% solution or optionally in solid form. A suitable ratio between the milk sample and the hydrogen peroxide is, for example, 5:1 when using 3% hydrogen peroxide, as the total volume of the milk sample and the reagent can be, for example, 50 ml. The vessel A is immediately fitted with the sample gauge C. The oxygen pressure that develops as a any function of the catalase activity is equalized by means of the tube E in the sample meter C while displacing the measuring liquid through the tube D. When the reaction ends after approx. 3 hours, the liquid level in the sample gauge C indicates the catalase activity and thus the quality of the milk.
Når kvalitetskontrollen er avsluttet overfores proven til analyse av melkens sammensetning. Av hensyn til dette er karet A utformet og dimensjonert på en slik måte at det passer inn i en kassett til den anvendte analysator. When the quality control is finished, the sample is transferred for analysis of the composition of the milk. For this reason, the vessel A is designed and dimensioned in such a way that it fits into a cassette for the analyzer used.
Den ovenfor beskrevne provemåler kan også erstattes med en i karet A's propp innsatte trykkmåler, som kan være forsynt med en slik skala at den angir direkte graden av katalaseaktiviteten, idet man samtidig bor ta hensyn til korreksjoner som folge av variasjoner i temperatur og atmosfæretrykk. The sample gauge described above can also be replaced with a pressure gauge inserted in vessel A's plug, which can be equipped with such a scale that it directly indicates the degree of catalase activity, while at the same time taking account of corrections as a result of variations in temperature and atmospheric pressure.
Claims (2)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI743168A FI51409C (en) | 1974-10-29 | 1974-10-29 | Ways to process samples for quality control of milk. |
Publications (3)
Publication Number | Publication Date |
---|---|
NO753636L NO753636L (en) | 1976-04-30 |
NO138738B true NO138738B (en) | 1978-07-24 |
NO138738C NO138738C (en) | 1978-11-01 |
Family
ID=8508096
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO753636A NO138738C (en) | 1974-10-29 | 1975-10-29 | PROCEDURE AND APPARATUS FOR MANAGING MILK SAMPLES FOR QUALITY CONTROL |
Country Status (6)
Country | Link |
---|---|
DE (1) | DE2548219C3 (en) |
DK (1) | DK142184B (en) |
FI (1) | FI51409C (en) |
GB (1) | GB1526813A (en) |
NO (1) | NO138738C (en) |
SE (1) | SE418016B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9018540D0 (en) * | 1990-08-23 | 1990-10-10 | Diversey Corp | Somatic cell counts |
-
1974
- 1974-10-29 FI FI743168A patent/FI51409C/en active
-
1975
- 1975-10-28 DE DE2548219A patent/DE2548219C3/en not_active Expired
- 1975-10-28 GB GB44388/75A patent/GB1526813A/en not_active Expired
- 1975-10-29 SE SE7512080A patent/SE418016B/en not_active IP Right Cessation
- 1975-10-29 DK DK486875AA patent/DK142184B/en unknown
- 1975-10-29 NO NO753636A patent/NO138738C/en unknown
Also Published As
Publication number | Publication date |
---|---|
FI51409B (en) | 1976-08-31 |
DE2548219A1 (en) | 1976-05-06 |
FI316874A (en) | 1976-04-30 |
DK486875A (en) | 1976-04-30 |
DE2548219B2 (en) | 1978-04-06 |
NO753636L (en) | 1976-04-30 |
GB1526813A (en) | 1978-10-04 |
DK142184B (en) | 1980-09-15 |
SE7512080L (en) | 1976-04-30 |
DE2548219C3 (en) | 1978-11-30 |
FI51409C (en) | 1976-12-10 |
SE418016B (en) | 1981-04-27 |
NO138738C (en) | 1978-11-01 |
DK142184C (en) | 1981-02-09 |
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