NL9401678A - Self-stabilizing loop method (Z.Z.S.L.). - Google Patents

Description

Self-stabilizing loop method (Z.Z.S.L.).

This is a basic concept for a new generation of medicines, consisting of protein mantles containing DNA / RNA strands, without mantle gene, which offer the possibility to exclude pieces of DNA outside the loop in the form of a loop. The closed DNA is not transcripted.

flé Z.Z.S.L · is formed by inserting a DNA / RNA strand (from viruses or self-synthesized) into the DNA of procaryotes or eucaryotes, forming a loop consisting of a portion of the introduced DNA and the inefficient operon or gene. The loop is stabilized by a binding protein (to be investigated), the gene of which lies on the inserted DNA strand. The binding protein's positive regulator is in front of its gene. The negative regulator is closed out in the loop together with the inefficient operon or gene, among other things. The good form of the inefficient operon or gene is applied to the respective DNA strand for the positive regulator. Behind the gene of the binding protein are a leader sequence, attenuat6r side, binding protein recognition sequence, negative regulator, attenuator side, leader sequence followed by a series of initiation signals, respectively.

The DNA / RNA strand is composed in such a way that it settles specifically for the inefficient operon or gene (or what one wants to exclude in the loop), by increasing the stability of the total DNA.

The manipulated DNA / RNA is contained in a protein mantle, the code of which does not appear on the DNA / RNA, as a result of which it cannot reproduce. For the production, procaryotes or eucaryotes where the mantle gene is incorporated, the concentration of which must be as high as possible * we can now insert the manipulated DNA / RNA into our manipulated cell. The result is a synthesized, no-coat gene suitable for use with the Z.Z.S.L.

Example of the application of the Z.Z.S.L. with Ecolie bacteria.

Suppose the lactose operon is mutated in one of structural genes, then the good form of the operon is introduced into an engineered virus followed by the sequences given above. This virus settles specifically for the lactose operon and excludes it. The RNA polymerase begins transcription on the lactose operon on the virus DNA. Then the positive regulator gene A1 transcript is followed by the transcription of the binding gene B. The binding gene B does not contain a normal termination side, but is followed by a leader sequence containing two fantasy codons. This positions the ribosomes, causing the leader messenger RNA to transmit a signal to the RNA polymerase to stop transcription. The RNA polymerase reaches the attenuator side and exits the DNA here. Because the RNA polymerase exits the DNA here, it does not reach the loop linked by the binding protein B, so that it does not endanger the loop.

Once the loop has been formed, transcription must be prevented. The RNA polymerase sqents the DNA into initiation signals at a rate of 1000 base pairs per second, therefore the final sequence of the virus DNA is required to consist of several initiation signals. As a result, the RNA polymerase is driven to one side, as it were. the virus DNA.

It is now necessary for the RNA polymerase to begin transcription, otherwise it will begin transcription of the inefficient operon. Hence, the initiation signals are followed by a leader sequence containing two fantasy codons and an attenuator side. This causes the RNA polymerase to fall off the virus DNA. (slide effect). The attenuator side is followed by the negative regulator A2 of the binding protein B.

We now see that A2 is located together with the recognition sequence of the binding protein B between two attenuator sides. As a result, no transcription of A2 takes place, so that binding protein B is not negatively regulated / then anchoring of the loop #

Using the Z.Z.S.L. we can switch off and build in pieces of DNA without damaging the organism concerned. Because this new medicine consists of DNA / RNA strands in a protein mantle (from nature or synthesized by ourselves), we can recognize and manipulate every cell (read host).

Claims (9)

This is a group of new genetically based drugs that can replace current antibiotics, CHARACTERIZED that it consists of DNA / RNA without a mantle gene, engineered to implant at a specific site and include the following sequences listed below order. li Any operon or gene to be replaced 2: A positive regulator of the binding protein. 3: A leader sequence 4; Attenuator side. 5: A recognition sequence for the binding protein. 6: A negative regulator for the binding protein. 7: Attenuator side. 8: Leader sequence. 9: An initiation rich sequence. Remarks The gene for the binding protein is located between the positive regulator and the leader sequence (between points 2 and 3). As a result, a loop is formed in the DNA of the host, consisting of a part of the virus DNA, namely your points 5 , 6,7,8,9, and the operon or gene of Your host. The manipulated DNA / RNA is contained in a protein mantle (from nature or synthesized by itself) that can recognize a host at any given target.