NL7809727A - Recovery of proteins from microorganisms and enzymes - has hydrolysis in aq. medium followed by ultrafiltration of the hydrolysate - Google Patents
Recovery of proteins from microorganisms and enzymes - has hydrolysis in aq. medium followed by ultrafiltration of the hydrolysate Download PDFInfo
- Publication number
- NL7809727A NL7809727A NL7809727A NL7809727A NL7809727A NL 7809727 A NL7809727 A NL 7809727A NL 7809727 A NL7809727 A NL 7809727A NL 7809727 A NL7809727 A NL 7809727A NL 7809727 A NL7809727 A NL 7809727A
- Authority
- NL
- Netherlands
- Prior art keywords
- proteins
- ultrafiltration
- enzymes
- hydrolysate
- protein
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 18
- 238000000108 ultra-filtration Methods 0.000 title claims abstract description 6
- 244000005700 microbiome Species 0.000 title claims abstract description 5
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 4
- 238000011084 recovery Methods 0.000 title abstract description 3
- 239000000413 hydrolysate Substances 0.000 title abstract 2
- 230000007062 hydrolysis Effects 0.000 title description 3
- 238000006460 hydrolysis reaction Methods 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract description 13
- 238000000746 purification Methods 0.000 claims description 3
- 239000012736 aqueous medium Substances 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 239000010802 sludge Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000002351 wastewater Substances 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims 2
- 229910021529 ammonia Inorganic materials 0.000 claims 1
- 239000002609 medium Substances 0.000 claims 1
- 150000001720 carbohydrates Chemical class 0.000 abstract description 3
- 235000014633 carbohydrates Nutrition 0.000 abstract description 3
- 102000015636 Oligopeptides Human genes 0.000 abstract description 2
- 108010038807 Oligopeptides Proteins 0.000 abstract description 2
- 235000014113 dietary fatty acids Nutrition 0.000 abstract description 2
- 229930195729 fatty acid Natural products 0.000 abstract description 2
- 239000000194 fatty acid Substances 0.000 abstract description 2
- 150000004665 fatty acids Chemical class 0.000 abstract description 2
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 230000007065 protein hydrolysis Effects 0.000 abstract 1
- 239000011343 solid material Substances 0.000 abstract 1
- 238000004065 wastewater treatment Methods 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000005905 Hydrolysed protein Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/18—Treatment of sludge; Devices therefor by thermal conditioning
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/001—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Thermal Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Hydrology & Water Resources (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Peptides Or Proteins (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
Description
3028 jr. -Λ 13028 yrs -Λ 1
STAMICARBON B.V. te GELEENSTAMICARBON B.V. to YELLOW
WERKWIJZE VOOR HET WINNEN VAN EIWITTENPROCESS FOR WINNING PROTEINS
De uitvinding heeft betrekking op een werkwijze voor het winnen van eiwitten door microorganismen en/of enzymen bij verhoogde temperatuur in waterig milieu te behandelen onder vorming vein een hydrolysaat.The invention relates to a method of recovering proteins by treating microorganisms and / or enzymes at an elevated temperature in an aqueous medium to form a hydrolyzate.
Een dergelijke werkwijze is onder meer bekend uit de niet voor-5 gepubliceerde Nederlandse octrooiaanvrage 7707081. In deze octrooiaanvrage is beschreven dat het mogelijk is microorganismen in de vorm van aktief slib afkomstig uit een zuiveringsinstallatie bij verhoogde temperatuur in aanwezigheid van NH^ te hydrolyseren.Such a method is known, inter alia, from Netherlands Patent Application 7707081, not pre-published. In this patent application it is described that it is possible to hydrolyze microorganisms in the form of active sludge from a purification installation at elevated temperature in the presence of NH 2.
Het hydrolysaat dat gevormd wordt tijdens een dergelijke behan-10 deling bevat behalve eiwitten, koolhydraten en hydrolyseprodukten daarvan, grote hoeveelheden water. Voor een economische toepassing van vooral de eiwitten uit het hydrolysaat is het daarom noodzakelijk dit water geheel of grotendeels te verwijderen.The hydrolyzate formed during such a treatment contains, besides proteins, carbohydrates and hydrolysis products thereof, large amounts of water. For an economic application of especially the proteins from the hydrolyzate it is therefore necessary to completely or largely remove this water.
In dit verband wordt onder de term eiwit niet alleen eiwit zelf 15 verstaan, maar ook gedenatureerd eiwit, hydrolyseprodukten van eiwit, zoals oligopeptiden, en mengsels van bovengenoemde produkten met koolhydraten, vetzuren en dergelijke.In this connection, the term protein is understood to include not only protein itself, but also denatured protein, hydrolysis products of protein, such as oligopeptides, and mixtures of the above products with carbohydrates, fatty acids and the like.
Een mogelijke oplossing voor bovengenoemd probleem zou zijn het water volledig te verdampen. De daarvoor benodigde hoeveelheid energie is 20 echter zo groot, dat een dergelijke werkwijze niet aantrekkelijk is.A possible solution to the above problem would be to evaporate the water completely. However, the amount of energy required for this is so great that such a method is not attractive.
Verrassenderwijs is nu gevonden, dat het mogelijk is eiwit, zoals hierboven gedefinieerd, met grote zuiverheid en nagenoeg kwantitatief te winnen uit het hydrolysaat.Surprisingly, it has now been found that it is possible to recover protein, as defined above, with high purity and substantially quantitatively from the hydrolyzate.
Volgens de uitvinding is een werkwijze voor het winnen van eiwit 25 daardoor gekenmerkt, dat men het hydrolysaat, na afscheiding van eventueel aanwezige vaste stof, door middel van ultrafiltratie concentreert.According to the invention, a protein recovery method is characterized in that the hydrolyzate is concentrated by ultrafiltration after separation of any solid present.
Bij de werkwijze volgens de uitvinding gaat men bij voorkeur uit 2 afvalwaterzuiveringsinstallatie in aanwezigheid van NH^ te hydrolyseren.In the process according to the invention it is preferred to hydrolyze from 2 waste water purification plant in the presence of NH 2.
Het concentraat, dat verkregen is na de ultrafiltratie kan men, afhankelijk van de gewenste toepassing op velerlei wijze verder verwerken.Depending on the desired application, the concentrate obtained after the ultrafiltration can be further processed in many ways.
Als een volledig droog produkt gewenst is, is het mogelijk het concentraat 5 aan een drooginstallatie, b.v. een sproeidroger toe te voeren. Een andere mogelijkheid is om het eiwit in het concentraat te coaguleren, en aansluitend af te scheiden en te drogen.If a completely dry product is desired, it is possible to add the concentrate 5 to a drying installation, e.g. to supply a spray dryer. Another possibility is to coagulate the protein in the concentrate, and subsequently separate and dry it.
Het is bijzonder verrassend, dat het mogelijk is op deze wijze het gehydrolyseerde eiwit nagenoeg kwantitatief af te scheiden, aangezien 10 ultracentrifuge metingen Rebben uitgewezen, dat het molecuulgewicht (Mw) van het gehydrolyseerde eiwit ongeveer 5000 bedraagt, terwijl algemeen bekend is, dat ultrafiltratie in feite niet of nauwelijks geschikt is voor het concentreren van dergelijke laagmolekulaire produkten.It is particularly surprising that it is possible to separate the hydrolysed protein almost quantitatively in this manner, since 10 ultracentrifuge measurements Rebben indicated that the molecular weight (Mw) of the hydrolysed protein is approximately 5,000, while it is well known that ultrafiltration in is in fact hardly suitable for concentrating such low-molecular products.
78097277809727
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL7809727A NL7809727A (en) | 1978-09-26 | 1978-09-26 | Recovery of proteins from microorganisms and enzymes - has hydrolysis in aq. medium followed by ultrafiltration of the hydrolysate |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL7809727A NL7809727A (en) | 1978-09-26 | 1978-09-26 | Recovery of proteins from microorganisms and enzymes - has hydrolysis in aq. medium followed by ultrafiltration of the hydrolysate |
| NL7809727 | 1978-09-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL7809727A true NL7809727A (en) | 1980-03-28 |
Family
ID=19831609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL7809727A NL7809727A (en) | 1978-09-26 | 1978-09-26 | Recovery of proteins from microorganisms and enzymes - has hydrolysis in aq. medium followed by ultrafiltration of the hydrolysate |
Country Status (1)
| Country | Link |
|---|---|
| NL (1) | NL7809727A (en) |
-
1978
- 1978-09-26 NL NL7809727A patent/NL7809727A/en not_active Application Discontinuation
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| Date | Code | Title | Description |
|---|---|---|---|
| A1B | A search report has been drawn up | ||
| BV | The patent application has lapsed |