NL2032682B1 - Combined orifice plate frame for molecular experiments of crops - Google Patents
Combined orifice plate frame for molecular experiments of crops Download PDFInfo
- Publication number
- NL2032682B1 NL2032682B1 NL2032682A NL2032682A NL2032682B1 NL 2032682 B1 NL2032682 B1 NL 2032682B1 NL 2032682 A NL2032682 A NL 2032682A NL 2032682 A NL2032682 A NL 2032682A NL 2032682 B1 NL2032682 B1 NL 2032682B1
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- NL
- Netherlands
- Prior art keywords
- plate
- plate bodies
- bodies
- orifice plate
- orifice
- Prior art date
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/02—Water baths; Sand baths; Air baths
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
- B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/023—Adapting objects or devices to another adapted for different sizes of tubes, tips or container
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/025—Align devices or objects to ensure defined positions relative to each other
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/028—Modular arrangements
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1894—Cooling means; Cryo cooling
Landscapes
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Disclosed is a combined orifice plate frame for molecular experiments of crops, which comprises an orifice plate container which is open at the top and is internally provided with supporting parts; first plate bodies, in which 96-orifice plate areas and EP drug pipe orifices are formed, and which are placed in the orifice plate container and supported by supporting surfaces of the supporting parts; and second plate bodies, which are 144-orifice plates, have the same size with the first plate bodies, are stacked in the orifice plate container and are located on the first plate bodies, wherein the number of the first plate bodies and the number of the second plate bodies are respectively at least two, and the first plate bodies and the second plate bodies can be fast switched according to experimental needs. The present invention discloses and provides the combined orifice plate frame for the molecular experiments of the crops. Since the orifice plate container is internally provided with at least two first plate bodies and at least two second plate bodies, different types of orifice plates are fast switched according to different experimental needs; and the stacked plate bodies can be unfolded on the basis, so that needs of different experimental conditions can be met.
Description
COMBINED ORIFICE PLATE FRAME FOR MOLECULAR EXPERIMENTS OF CROPS
The present invention relates to the technical field of molecular experiment devices of crops, and more particularly relates to a combined orifice plate frame for molecular experiments of crops.
At present, different types of EP (Epoxy Resin) pipes and 96-orifice plates are used for GMO (Genetically Modified Organism) detection of crops, extraction of DNA and RNA, and PCR (Polymerase Chain Reaction) pre-treatment, and in order to achieve the stability and accuracy of operation, the EP pipes and the 96-orifice plates are usually placed into orifice plate frames for experimental operation. However, different orifice plate frames are selected according to different experiment quantities and different test contents, and the orifice plate frames are diversified and are not universal, leading to inconvenience in use.
Therefore, how to provide a combined orifice plate frame for molecular experiments of crops is a problem to be solved urgently by those skilled in the art.
For the purpose, the present invention aims to provide a combined orifice plate frame for molecular experiments of crops, which solves the problem that the existing orifice plate frames are not universal and cannot meet using needs of experiments.
The present invention provides the combined orifice plate frame used for the molecular experiments of the crops, comprising: an orifice plate container, which is open at the top and is internally provided with supporting parts; first plate bodies, in which 96-orifice plate areas and EP drug pipe orifices are formed, and which are placed in the orifice plate container and supported by supporting surfaces of the supporting parts; and second plate bodies, which are 144-orifice plates, have the same size with the first plate bodies, are stacked in the orifice plate container and are located on the first plate bodies, wherein the number of the first plate bodies and the number of the second plate bodies are respectively at least two, and the first plate bodies and the second plate bodies can be fast switched according to experimental needs.
It can be known from the above technical solution that, compared with the prior art, the present invention discloses and provides the combined orifice plate frame used for the molecular experiments of the crops; as the orifice plate container is internally provided with at least two first plate bodies and at least two second plate bodies, different types of orifice plates are fast switched according to different experimental needs; and the stacked plate bodies can be unfolded on the basis, so that needs of different experimental conditions can be met.
Further, the transverse spacing between adjacent orifices in the second plate bodies is 0.45- 0.55 cm, and the longitudinal spacing between the two adjacent orifices is 1.45-1.55 cm.
Further, each first plate body has four orifice plate areas, and 96 through holes are formed in each area.
Further, a plurality of EP drug pipe orifices with a diameter of 1.1 cm are formed between the two adjacent areas.
Further, the spacing between adjacent orifices in each orifice plate area is 1.95 cm-2.05 cm.
Further, the supporting parts are a plurality of supporting pillars extending upwards from the bottom of the orifice plate container 1, and the top of each supporting pillar is a supporting surface.
Further, the height of each supporting pillar is greater than 2 cm, and an ice bath space is formed below the supporting surface thereof and above the bottom wall of the orifice plate container, which is used for containing ice water.
Further, the orifice plate container, the first plate bodies and the second plate bodies are made of transparent materials.
Further, the orifice plate container, the first plate bodies and the second plate bodies are made of Acrylic.
To more clearly describe the technical solutions in embodiments of the present invention or in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be simply presented below. Apparently, the drawings in the following description are merely embodiments of the present invention, and for those ordinary skilled in the art, other drawings can also be obtained according to these drawings without contributing creative labor.
Fig. 1 is an exploded view of an embodiment of a combined orifice plate frame for molecular experiments of crops, which is provided by the present invention;
Fig. 2 is an assembly diagram of the embodiment of the combined orifice plate frame used for the molecular experiments of the crops, which is provided by the present invention;
Fig. 3 shows a structural schematic diagram of an orifice plate container;
Fig. 4 shows a structural schematic diagram of a second plate body; and
Fig. 5 shows a structural schematic diagram of a first plate body.
Embodiments of the present invention will be described below in detail. Examples of the embodiments are shown in drawings, wherein same or similar reference signs refer to same or similar elements or elements having same or similar functions from beginning to end.
Embodiments described below by reference to the drawings are exemplary embodiments, and are used for explaining the present invention, and shall not be understood as a limitation to the present invention.
It should be understood in the description of the present invention that terms such as “upper”, “lower”, “front”, “rear”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inner”, “outer”, etc. indicate direction or position relationships shown based on the drawings, and are only intended to facilitate the description of the present invention and simplify the description rather than to indicate or imply that the indicated device or element must have a specific direction or constructed and operated in a specific direction, and therefore, shall not be understood as a limitation to the present invention.
In the present invention, unless otherwise specifically regulated and defined, terms such as “installation”, “connected”, “connecting”, “fixation” and the like shall be understood in broad sense, and for example, may refer to fixed connection or detachable connection or integral connection, may refer to mechanical connection or electrical connection, and may refer to direct connection or indirect connection through an intermediate medium or inner communication of two elements or interaction relationship of two elements. For those ordinary skilled in the art, the specific meanings of the above terms in the present invention may be understood according to concrete conditions.
In the present invention, unless otherwise clearly specified and defined, a first feature is “above” or “below” a second feature comprises that the first feature and the second feature come into direct contact or the first feature and the second feature come into contact through additional features thereof instead of direct contact. Moreover, the first feature is “on”, “above” and “over” the second feature comprises that the first feature is directly above or slightly above the second feature, or just indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature is “under”, “below” and “beneath” the second feature comprises that the first feature is directly below or slightly below the second feature, or just indicates that the horizontal height of the first feature is lower than that of the second feature.
In the prior art, in order to achieve the stability and accuracy of operation, different types of
EP pipes and 96-orifice plates are usually placed into orifice plate frames for experimental operation. Since the orifice plate frames are diversified, different orifice plate frames are selected according to different experimental quantities and different test contents, which is unfavourable for use in experiments. Therefore, embodiments of the present invention disclose a combined orifice plate frame for molecular experiments of crops. With reference to Figs. 1 - 3, the combined orifice plate frame may comprise an orifice plate container 1 and plate bodies; advantageously, the orifice plate container 1 is a container which is open at the top, may be a square box body, a rectangular box body, a round box body or a polygonal box body, and is internally provided with supporting parts 11; and the plate bodies comprise first plate bodies 2 and second plate bodies 3, and the plate bodies are placed into the orifice plate container 1.
Specifically, 96-orifice plate areas 21 and EP drug pipe orifices 22 are formed in the first plate bodies 2; the first plate bodies 2 are placed into the orifice plate container 1 and are supported by supporting surfaces of the supporting parts 11; the second plate bodies 3 are 144-orifice plates, have the same size with the first plate bodies 2, are stacked in the orifice plate container 1 and are located on the first plate bodies 2; and the aperture of the first plate body 2 is different from that of the second plate body 3, so that placing needs of different experimental samples can be met.
More advantageously, the number of the first plate bodies 2 and the number of the second plate bodies 3 are respectively at least two; the first plate bodies 2 and the second plate bodies 3 can be fast switched according to experimental needs, or the same orifice plates are unfolded, so that the quantity of the contained samples can be increased; and in the above embodiment, using needs of the experiments can be met by expanding or combining the orifice plate frames.
Advantageously, with reference to Fig. 4, the transverse spacing between two adjacent orifices in the second plate bodies 3 is 0.45 - 0.55 cm, and the longitudinal spacing between the two adjacent orifices is 1.45-1.55 cm, so as to ensure that two adjacent sample pipes cannot collide to each other when lids of the sample pipes are closed or opened.
In some embodiments of the present invention, each first plate body 2 has four orifice plate areas 21; 96 through holes are formed in each area 21; and a plurality of EP drug pipe orifices 22 with a diameter of 1.1 cm are formed between the two adjacent areas 21, thereby increasing the quantity of the contained sample pipes and meeting fixing needs of different EP drug pipes.
Advantageously, the spacing between the two adjacent orifices in each orifice plate area 21 is 1.95 cm-2.05 cm.
In some embodiments of the present invention, with reference to Fig. 3, the supporting parts 11 are a plurality of supporting pillars extending upwards from the bottom of the orifice plate container 1, and the top of each supporting pillar is the supporting surface. The cross section of each supporting pillar may be rectangular, round or other shapes.
In some other embodiments, the supporting parts 11 may further be placing platforms arranged on the inner wall of the orifice plate container 1 or may be other structure forms such as supporting plates and the like, which are used for supporting the plate bodies at the upper parts.
Advantageously, the height of each supporting pillar is greater than 2 cm, and an ice bath space is formed below the supporting surface thereof and above the bottom wall of the orifice plate container 1, which is used for containing ice water; and an ice bath condition can be provided, and different experimental needs are met.
In some other embodiments, the orifice plate container 1, the first plate bodies 2 and the second plate bodies 3 are made of transparent materials, so as to be convenient for observation, thereby greatly improving the accuracy of operation in an experimental process. The orifice plate container 1, the first plate bodies 2 and the second plate bodies 3 may be made of acrylic, so that the service life is longer than that of the traditional PE plate.
In a specific embodiment of the present invention, each box body is a square with length, width and height of 31 x 20 x 6 cm; four supporting pillars are arranged at four corners; the height of each supporting pillar is 2 cm; the bottom of the bax body is well sealed, which can be used for containing ice water. The number of the second plate bodies is two; 144 orifices are formed in 5 each second plate body; the transverse spacing between two adjacent orifices is 0.5 cm; and the longitudinal spacing between the two adjacent orifices is 1.5 cm. The number of the first plate bodies is also two; each area has four orifice plate areas; and 96 orifices are formed in each area.
Orifices with the diameter of 1.1 cm may be formed in the middle parts of each plate body, in which EP drug pipes with the capacities of 1.5 ml and 2.0 ml can be placed. An ice bath space may be a space with the length, the width and the height of 30 x 20 x 2 cm.
The combined orifice plate frame of the present invention is a high-throughput combined orifice plate frame integrated with different types of EP pipe frames and different types of 96- orifice plate frames and can contain more samples. Compared with the traditional orifice plate frames and the traditional double-sided board, the orifice plate frame of the present invention has the following advantages: the quantity of the contained samples is increased by above 5 times than the traditional quantity; the samples can be observed more intuitively, so that the accuracy of operation is improved; different types of orifice plate frames can be fast switched for use; the needs of different experimental conditions can be met; the transverse spacing and the longitudinal spacing are designed scientifically and reasonably, so that the sample pipes cannot collide to each other; and the service life is long.
In the illustration of this description, the illustration of reference terms “one embodiment”, “some embodiments”, “example”, “specific example” or “some examples”, etc. means that specific features, structures, materials or characteristics illustrated in combination with the embodiment or example are included in at least one embodiment or example of the present invention. In this description, exemplary statements for the above terms do not must aim at the same embodiment or example. Moreover, the described specific features, structures, materials or characteristics can be combined appropriately in any one or more embodiments or examples. In addition, those skilled in the art can combine and integrate different embodiments or examples illustrated in this description.
Although the embodiments of the present invention have been shown and described above, it will be appreciated that the above embodiments are exemplary and shall not be understood as limitations to the present invention. Those ordinary skilled in the art can make changes, amendments, replacements and variations to the above embodiments within the scope of the present invention.
Claims (9)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210220434.7A CN114558634A (en) | 2022-03-08 | 2022-03-08 | Combined pore plate frame for crop molecular experiment |
Publications (1)
Publication Number | Publication Date |
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NL2032682B1 true NL2032682B1 (en) | 2023-01-30 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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NL2032682A NL2032682B1 (en) | 2022-03-08 | 2022-08-04 | Combined orifice plate frame for molecular experiments of crops |
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CN (1) | CN114558634A (en) |
NL (1) | NL2032682B1 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114558634A (en) * | 2022-03-08 | 2022-05-31 | 吉林省农业科学院 | Combined pore plate frame for crop molecular experiment |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4436473A1 (en) * | 1994-09-08 | 1996-03-14 | Bernd Dr Steinbrenner | Device for providing a multitude of objects on a carrier |
US6098819A (en) * | 1997-09-26 | 2000-08-08 | Eppendorf-Netheler-Hinz Gmbh | Magazine for pipette tips |
EP2623204A1 (en) * | 2012-02-03 | 2013-08-07 | F. Hoffmann-La Roche AG | Sample handling system |
CN114558634A (en) * | 2022-03-08 | 2022-05-31 | 吉林省农业科学院 | Combined pore plate frame for crop molecular experiment |
-
2022
- 2022-03-08 CN CN202210220434.7A patent/CN114558634A/en active Pending
- 2022-08-04 NL NL2032682A patent/NL2032682B1/en active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4436473A1 (en) * | 1994-09-08 | 1996-03-14 | Bernd Dr Steinbrenner | Device for providing a multitude of objects on a carrier |
US6098819A (en) * | 1997-09-26 | 2000-08-08 | Eppendorf-Netheler-Hinz Gmbh | Magazine for pipette tips |
EP2623204A1 (en) * | 2012-02-03 | 2013-08-07 | F. Hoffmann-La Roche AG | Sample handling system |
CN114558634A (en) * | 2022-03-08 | 2022-05-31 | 吉林省农业科学院 | Combined pore plate frame for crop molecular experiment |
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CN114558634A (en) | 2022-05-31 |
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