CN114558634A

CN114558634A - Combined pore plate frame for crop molecular experiment

Info

Publication number: CN114558634A
Application number: CN202210220434.7A
Authority: CN
Inventors: 孟令聪; 路明; 王敏; 郑淑波; 肖宏宇; 李穆; 高婷婷; 卢实; 岳尧海; 何欢; 周德龙; 黄威
Original assignee: Jilin Academy of Agricultural Sciences
Current assignee: Jilin Academy of Agricultural Sciences
Priority date: 2022-03-08
Filing date: 2022-03-08
Publication date: 2022-05-31
Also published as: NL2032682B1

Abstract

The invention relates to a combined pore plate frame for crop molecular experiments, which comprises: the orifice plate container is a container with an open top and is internally provided with a supporting part; a first plate body having a 96-well plate region and an EP medicine tube hole, placed in the well plate container, and supported by the support surface of the support portion; the second plate body is a 144-pore plate, has the same size as the first plate body, is stacked in the pore plate container and is positioned on the first plate body; first plate body and second plate body are two at least, can fast switch over according to the experiment needs. The invention discloses a combined orifice plate frame for molecular experiments of crops, wherein an orifice plate container is provided with at least two first plate bodies and two second plate bodies, so that different types of orifice plates can be quickly switched according to different experimental requirements, and stacked plate bodies can be unfolded on the basis, thereby meeting the requirements of different experimental conditions.

Description

Combined pore plate frame for crop molecular experiment

Technical Field

The invention relates to the technical field of crop molecular experiment devices, in particular to a combined perforated plate frame for crop molecular experiments.

Background

At present, different types of EP tubes and 96-well plates are used in crop transgenic detection, DNA and RNA extraction and PCR pretreatment, and are usually put into a well plate frame for experimental operation for operation stability and accuracy. However, different porous plate frames need to be selected according to the size of the experimental amount and different experimental contents, and the porous plate frames are various and have no universality, so that the use is inconvenient.

Therefore, how to provide a combined pore plate frame for molecular experiments of crops is a problem that needs to be solved by those skilled in the art.

Disclosure of Invention

Therefore, the invention aims to provide a combined orifice plate frame for a crop molecular experiment, and solves the problems that the existing orifice plate frame is not universal and cannot meet the use requirement of the experiment.

The invention provides a combined pore plate frame for crop molecular experiments, which comprises:

the orifice plate container is a container with an open top and is internally provided with a supporting part;

a first plate body having a 96-well plate area and an EP medicine tube hole thereon, placed in the well plate accommodator, and supported by the support surface of the support portion;

the second plate body is a 144-pore plate, has the same size as the first plate body, is stacked in the pore plate container and is positioned on the first plate body;

the first plate body with the second plate body is two at least, can fast switch over according to the experiment needs.

According to the technical scheme, compared with the prior art, the combined orifice plate frame for the crop molecular experiment is provided, the orifice plate container is provided with the at least two first plate bodies and the two second plate bodies, so that different types of orifice plates can be quickly switched according to different experiment requirements, and the stacked plate bodies can be unfolded on the basis, so that the requirements of different experiment conditions can be met.

Furthermore, adjacent holes on the second plate body are transversely spaced by 0.45-0.55cm, and longitudinally spaced by 1.45-1.55 cm.

Further, it has four orifice plate regions equally to divide on the first plate body, has all seted up 96 through-holes on each region.

Further, a plurality of EP medicine tube holes with the diameter of 1.1cm are arranged between the adjacent areas.

Further, the distance between adjacent holes in each hole plate area is 1.95cm-2.05 cm.

Further, the supporting parts are a plurality of supporting columns extending upwards from the bottom of the orifice plate container 1, and the tops of the supporting columns are supporting surfaces.

Further, the height of the support column is more than 2cm, and an ice bath space is formed below the support surface and in front of the bottom wall of the orifice plate container and is used for containing ice water.

Further, the orifice plate container, the first plate body and the second plate body are all made of transparent materials.

Further, the orifice plate container, the first plate body and the second plate body are made of acrylic.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the prior art descriptions will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is an exploded view of an embodiment of a combined porous plate rack for molecular experiments on crops according to the present invention;

FIG. 2 is an assembly view of an embodiment of the combined porous plate frame for molecular experiments on crops according to the present invention;

FIG. 3 is a schematic diagram of the construction of the orifice plate receptacle;

fig. 4 shows a schematic structural view of the second plate body;

fig. 5 shows a schematic structural diagram of the first plate body.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless expressly stated or limited otherwise, the recitation of a first feature "on" or "under" a second feature may include the recitation of the first and second features being in direct contact, and may also include the recitation that the first and second features are not in direct contact, but are in contact via another feature between them. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

Because in the prior art, different models of EP tubes and 96-well plates are usually put into a well plate rack for experimental operation for operation stability and accuracy. Because the kind of orifice plate frame is many, need be according to the size of the volume of experiment and the different orifice plate frames of the different choices of experimental content, be unfavorable for the experiment and use. Therefore, the embodiment of the present invention discloses a combined orifice plate frame for molecular experiments of crops, which can include an orifice plate container 1 and a plate body, and it is advantageous that the orifice plate container 1 is a container with an open top, and can be a square box, a rectangular box, a circular box, or a polygonal box, and a supporting part 11 is provided therein; the plate body has a first plate body 2 and a second plate body 3, which are placed into the well plate receptacle 1.

Specifically, a first plate 2, wherein the first plate 2 has a 96-well plate area 21 and an EP medicine tube hole 22, and is placed in the well plate container 1 and supported by a supporting surface of the supporting portion 11; the second plate body 3 is a 144-pore plate, has the same size as the first plate body 2, is stacked in the pore plate container 1, and is positioned on the first plate body 2; the first plate body 2 and the second plate body 3 have different apertures, so that the placing requirements of different experimental samples can be met;

more advantageously, the first plate body 2 and the second plate body 3 are at least two, and can be switched rapidly according to the experiment needs, or the same well plate is unfolded, so that the number of the samples to be accommodated can be increased, and the above embodiment can meet the use requirements of the experiment by expanding or combining the well plate racks.

Advantageously, with reference to fig. 4, the adjacent holes of the second plate body 3 are spaced apart by 0.45-0.55cm in the transverse direction and by 1.45-1.55cm in the longitudinal direction; this ensures that no collision occurs between adjacent sample tubes when the sample tubes close and open the lid.

In some embodiments of the present invention, the first plate 2 has four well plate areas 21, each area 21 has 96 through holes, and a plurality of EP medicine tube holes 22 with a diameter of 1.1cm are formed between adjacent areas 21. Therefore, the number of the sample tubes is increased, and the fixing requirements of different EP medicine tubes are met. Advantageously, the distance between adjacent holes in each of the perforated plate areas 21 is between 1.95cm and 2.05 cm.

In some embodiments of the present invention, referring to fig. 3, the supporting portion 11 is a plurality of supporting columns extending upward from the bottom of the orifice plate container 1, and the top of the supporting columns is a supporting surface. The cross section of the support column can be rectangular, can be circular, and can also be in other shapes.

In other embodiments, the supporting portion 11 may also be a placing table disposed on the inner wall of the orifice plate container 1, or other supporting plates, etc. for supporting the upper plate body.

Advantageously, the support column has a height greater than 2cm and forms an ice bath space below its support surface and in front of the bottom wall of the well plate container 1 for holding ice water; can provide ice bath conditions and meet different experimental requirements.

In other embodiments, the orifice plate container 1, the first plate body 2 and the second plate body 3 are made of transparent materials, so that observation is facilitated, and the operation accuracy is greatly improved in the experiment process. The orifice plate container 1, the first plate body 2 and the second plate body 3 may be made of acryl, and have a long life compared to a conventional PE plate.

In a specific embodiment of the invention, the box body is square, and has the length, width and height of 31X20X6cm, 4 support columns are arranged in four corners, the height of each support column is 2cm, the bottom of the box body is well sealed, and ice water can be contained in the box body. The two second plate bodies are provided with 144 holes, the hole distance is 0.5cm in the transverse direction and 1.5cm in the longitudinal direction, the two first plate bodies are provided, each first plate body is provided with four hole plate areas, each area is provided with 96 holes, the middle of each plate body can be provided with a hole with the diameter of 1.1cm, and the EP medicine tube placement with the capacity of 1.5ml and 2.0ml can be met. The ice bath space may be a space having a length, width and height of 30X20X2 cm.

The invention relates to a high-flux combined pore plate frame integrating EP pipe frames and 96 pore plate frames with different models, which can accommodate more samples. Compared with the traditional pore plate frame and a double-sided board, the pore plate frame has the following advantages: the number of samples contained increased more than 5 times the conventional one. The accuracy of operation can be improved by observing the sample more intuitively. The rapid switching use of different types of pore plate frames can be realized. Can meet the requirements of different experimental conditions. The design of the distance between the transverse holes and the longitudinal holes is scientific and reasonable, and the sample tubes cannot collide. The service life is long.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims

1. The utility model provides a combination orifice grillage for crops molecular experiment which characterized in that includes:

the orifice plate container (1), the orifice plate container (1) is a container with an open top, and a support part (11) is arranged in the orifice plate container;

a first plate body (2), wherein the first plate body (2) is provided with a 96-well plate area (21) and an EP medicine pipe hole (22), is placed in the well plate container (1) and is supported by the supporting surface of the supporting part (11);

the second plate body (3) is a 144-pore plate, is the same as the first plate body (2) in size, is stacked in the pore plate container (1), and is positioned on the first plate body (2);

the first plate body (2) and the second plate body (3) are at least two, and can be rapidly switched according to experiment needs.

2. The combined perforated plate rack for crop molecular experiments as claimed in claim 1, characterized in that adjacent holes on the second plate body (3) are spaced 0.45-0.55cm apart in the transverse direction and 1.45-1.55cm apart in the longitudinal direction.

3. The combined orifice-plate frame for molecular experiments on crops as claimed in claim 1, wherein four orifice-plate regions (21) are uniformly distributed on the first plate body (2), and each region (21) is provided with 96 through holes.

4. A combined aperture plate frame for molecular testing of crops as claimed in claim 3 wherein there are a plurality of EP medicine tube apertures (22) of 1.1cm diameter between adjacent said areas (21).

5. A combined aperture plate frame for molecular testing of crops as claimed in claim 3 wherein the distance between adjacent apertures on each aperture plate zone (21) is 1.95cm-2.05 cm.

6. A combined aperture plate rack for molecular experiments on crops as claimed in any of claims 1-5 wherein said support portion (11) is a plurality of support posts extending upwardly from the bottom of said aperture plate receptacle (1) and the top of said support posts is a support surface.

7. The combined orifice plate rack for crop molecular experiments as claimed in claim 6, wherein the height of the supporting column is greater than 2cm, and an ice bath space is formed below the supporting surface and in front of the bottom wall of the orifice plate container (1) for containing ice water.

8. A combined plate rack for molecular testing of crops as claimed in any of claims 1-5, wherein said plate holder (1), said first plate (2) and said second plate (3) are made of transparent material.

9. The combined orifice plate rack for crop molecular experiments according to claim 8, characterized in that the orifice plate holder (1), the first plate body (2) and the second plate body (3) are made of acrylic.