NL2028742B1 - Fluorescence immunochromatographic kit for simultaneous detection of four kinds of nitrofuran metabolites, preparation method and application thereof - Google Patents

Fluorescence immunochromatographic kit for simultaneous detection of four kinds of nitrofuran metabolites, preparation method and application thereof

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Publication number
NL2028742B1
NL2028742B1 NL2028742A NL2028742A NL2028742B1 NL 2028742 B1 NL2028742 B1 NL 2028742B1 NL 2028742 A NL2028742 A NL 2028742A NL 2028742 A NL2028742 A NL 2028742A NL 2028742 B1 NL2028742 B1 NL 2028742B1
Authority
NL
Netherlands
Prior art keywords
quantum dot
kinds
bsa
antibody
coupled
Prior art date
Application number
NL2028742A
Other versions
NL2028742A (en
Inventor
Yu Jianyang
Wang Hong
Lin Hong
Cheng Yuanyuan
Liu Xiuying
Guo Xiaohua
Gao Xue
Lao Minjun
Tang Yiwei
Li Xuepeng
Li Jianrong
Original Assignee
Univ Bohai
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Bohai filed Critical Univ Bohai
Publication of NL2028742A publication Critical patent/NL2028742A/en
Application granted granted Critical
Publication of NL2028742B1 publication Critical patent/NL2028742B1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9446—Antibacterials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2430/00—Assays, e.g. immunoassays or enzyme assays, involving synthetic organic compounds as analytes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Nanotechnology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The present invention provides a fluorescence immunochromatographic kit for simultaneous detection of four kinds of nitrofuran metabolites, a preparation method and an application thereof, and belongs to the technical field of antibiotic detection. The kit comprises a chromatographic test strip and quantum dot probes. A nitrocellulose membrane of the chromatographic test strip is provided with test lines T1, T2, T3, and T4 and a control line C; the test lines T1, T2, T3, and T4 are spray—coated with envelope antigens 2—NPAOZ—BSA, 2—NPAHD— BSA, 2—NPSEM—BSA, and 2—NPAMOZ—BSA, respectively; and the quantum dot probes comprise red light quantum dot—coupled 2—NPAOZ antibody, yellow light quantum dot-coupled 2-NPAHD antibody, green light quantum dot—coupled 2—NPSEM antibody, and orange light quantum dot—coupled 2—NPAMOZ antibody. The kit of the present invention can simultaneously detect four kinds of nitrofuran metabolites, with high sensitivity.
NL2028742A 2021-04-25 2021-07-15 Fluorescence immunochromatographic kit for simultaneous detection of four kinds of nitrofuran metabolites, preparation method and application thereof NL2028742B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110446512.0A CN113156112A (en) 2021-04-25 2021-04-25 Fluorescence immunochromatography kit for simultaneously detecting four nitrofuran metabolites and preparation method and application thereof

Publications (2)

Publication Number Publication Date
NL2028742A NL2028742A (en) 2021-09-13
NL2028742B1 true NL2028742B1 (en) 2022-04-08

Family

ID=76870544

Family Applications (1)

Application Number Title Priority Date Filing Date
NL2028742A NL2028742B1 (en) 2021-04-25 2021-07-15 Fluorescence immunochromatographic kit for simultaneous detection of four kinds of nitrofuran metabolites, preparation method and application thereof

Country Status (2)

Country Link
CN (1) CN113156112A (en)
NL (1) NL2028742B1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116478685B (en) * 2023-05-11 2024-03-29 安徽工业大学 Fluorescent probe for synchronously quantifying multiple phosphorus-containing substances, preparation method and device

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101196523A (en) * 2007-12-29 2008-06-11 华中农业大学 Chicken infectious Fabricius bursa immune body immune colloidal gold fast detecting and its application
CN103048460B (en) * 2012-12-15 2015-01-21 武汉珈源生物医学工程有限公司 Method for detecting by using quantum dot fluorescence immunochromatographic test strips
CN106248976A (en) * 2015-06-06 2016-12-21 北京艾旗斯德科技有限公司 Four kinds of metabolites of nitrofuran colloidal gold strips of a kind of detection and preparation method thereof
CN106053787A (en) * 2016-06-06 2016-10-26 上海师范大学 Fluorescent immunochromatography test strip for detecting furazolidone metabolites as well as preparation and application
CN107315091A (en) * 2017-06-29 2017-11-03 重庆师范大学 One kind detection Cistofuran metabolite quantum dot immune chromatographic test paper, preparation method and applications
CN207650216U (en) * 2017-11-14 2018-07-24 重庆师范大学 Quantum dot fluorescence duplex test card that is a kind of while detecting furantoin and Furaxone metabolite
CN109239328A (en) * 2018-09-26 2019-01-18 南京财经大学 A kind of immunochromatographic method of four kinds of metabolites of nitrofuran of quick detection
CN109884293A (en) * 2019-03-29 2019-06-14 中国海洋大学 A kind of food allergen rapid detection method based on quantum dot fluorescence
CN112326959A (en) * 2020-12-10 2021-02-05 南昌大学 Preparation and detection device of multicolor fluorescence signal opening type competitive immunochromatography test strip

Also Published As

Publication number Publication date
NL2028742A (en) 2021-09-13
CN113156112A (en) 2021-07-23

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