CN116478685B - Fluorescent probe for synchronously quantifying multiple phosphorus-containing substances, preparation method and device - Google Patents
Fluorescent probe for synchronously quantifying multiple phosphorus-containing substances, preparation method and device Download PDFInfo
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- 239000007850 fluorescent dye Substances 0.000 title claims abstract description 87
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 239000011574 phosphorus Substances 0.000 title claims abstract description 43
- 229910052698 phosphorus Inorganic materials 0.000 title claims abstract description 43
- 239000000126 substance Substances 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 229910052984 zinc sulfide Inorganic materials 0.000 claims abstract description 97
- 238000001514 detection method Methods 0.000 claims abstract description 27
- DRDVZXDWVBGGMH-UHFFFAOYSA-N zinc;sulfide Chemical class [S-2].[Zn+2] DRDVZXDWVBGGMH-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 20
- 229910021645 metal ion Inorganic materials 0.000 claims abstract description 16
- 239000003086 colorant Substances 0.000 claims abstract description 7
- 238000001027 hydrothermal synthesis Methods 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 5
- 239000010949 copper Substances 0.000 claims description 41
- 239000000243 solution Substances 0.000 claims description 40
- 238000006243 chemical reaction Methods 0.000 claims description 27
- 239000011259 mixed solution Substances 0.000 claims description 27
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 27
- 239000012498 ultrapure water Substances 0.000 claims description 27
- 230000001360 synchronised effect Effects 0.000 claims description 22
- 238000011002 quantification Methods 0.000 claims description 21
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 239000000523 sample Substances 0.000 claims description 17
- 238000010791 quenching Methods 0.000 claims description 16
- 230000000171 quenching effect Effects 0.000 claims description 16
- 238000002189 fluorescence spectrum Methods 0.000 claims description 14
- DKIDEFUBRARXTE-UHFFFAOYSA-N 3-mercaptopropanoic acid Chemical compound OC(=O)CCS DKIDEFUBRARXTE-UHFFFAOYSA-N 0.000 claims description 12
- 238000004090 dissolution Methods 0.000 claims description 11
- 230000035484 reaction time Effects 0.000 claims description 11
- 238000005259 measurement Methods 0.000 claims description 9
- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical compound O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 claims description 6
- 238000000502 dialysis Methods 0.000 claims description 6
- 230000005284 excitation Effects 0.000 claims description 6
- 239000004246 zinc acetate Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 239000005083 Zinc sulfide Substances 0.000 claims description 4
- 125000002843 carboxylic acid group Chemical group 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 3
- 230000008569 process Effects 0.000 abstract description 6
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 29
- 230000008859 change Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 230000002194 synthesizing effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 2
- 235000011180 diphosphates Nutrition 0.000 description 2
- 238000001506 fluorescence spectroscopy Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000037182 bone density Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 201000008482 osteoarthritis Diseases 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- -1 phosphate compound Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/02—Use of particular materials as binders, particle coatings or suspension media therefor
- C09K11/025—Use of particular materials as binders, particle coatings or suspension media therefor non-luminescent particle coatings or suspension media
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- C09K11/661—Chalcogenides
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- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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Abstract
The invention discloses a fluorescent probe for synchronously quantifying a plurality of phosphorus-containing substances, a preparation method and a device thereof, relates to the technical field of food and environmental safety detection, and aims to solve the problems of single detection target, insufficient sensitivity and complex detection process of the existing phosphorus-containing substances; the method comprises the steps of preparing three zinc sulfide quantum dots with different colors by controlling the time of hydrothermal reaction and respectively doping different metal ions by using the same method to obtain three fluorescent probes: znS: cu + ‑cyan‑Fe 3+ 、ZnS:Cu + ‑yellow‑Sn 2+ 、ZnS:Cu + ‑orange‑Ni 2+ The three materials have no fluorescence under the irradiation of ultraviolet lamp, and are respectively recovered to cyan, yellow and orange red under the irradiation of ultraviolet lamp after reacting with PPi, pi, ATP; the fluorescent probe provided by the invention has the advantages of simple preparation method, convenience in detection operation and high sensitivity, various phosphorus-containing substances can be detected by the same method, and the detection device is convenient to carry and is beneficial to being applied to field detection of various phosphorus-containing substances in actual samples.
Description
Technical Field
The invention relates to the technical field of food and environmental safety detection, in particular to a fluorescent probe for synchronously quantifying various phosphorus-containing substances, a preparation method and a device thereof.
Background
Excess phosphate will cause damage to the organism, for example, interfere with the balance of phosphorus and calcium in the body, reduce bone density, and increase the risk of heart disease. Excess phosphate ions are released into the body of water, which promotes eutrophication of the body of water and causes phytoplankton and aquatic plants to bloom, which depletes dissolved oxygen levels, which in turn causes decay and death of the aquatic organisms and plants. Abnormal levels of pyrophosphate are associated with many diseases, such as osteoarthritis, arteriosclerosis, and cancer. Adenosine triphosphate is a phosphate compound that provides energy for the human body and plays an important role in the life activity of the human body. The in vivo phosphorus-containing substances have an important role in maintaining physiological and metabolic processes of the human body, considering that the contents of phosphate, pyrophosphate and adenosine triphosphate are important markers of human health. Once the phosphorus-containing substances are excessive, the health and the ecological environment safety of people are jeopardized through food chains and ecological circulation. However, most of the existing phosphorus-containing substance detection methods have single target, or have insufficient sensitivity, or complex detection process, so that detection work cannot be conveniently carried out on site; therefore, how to realize rapid and accurate detection of phosphorus-containing substances is a focus of attention.
Disclosure of Invention
Based on the above purpose, we designed and manufactured a device for synchronously quantifying multiple phosphorus-containing substances, which is based on three kinds of doped zinc sulfide quantum dots with different fluorescence, and because the zinc sulfide surface contains rich carboxylic acid groups, different metal ion quenchers are further modified on the multicolor quantum dot surface, so that corresponding multicolor fluorescence nanoprobes are successfully constructed, and then according to the difference of binding capacity between different phosphorus-containing substances and corresponding metal ions, the metal ions on the surfaces of the probe particles are induced to be released again, fluorescence is recovered, and further, the selective identification and synchronous distinction of three different phosphorus-containing substances (PPi, pi and ATP) is realized.
In order to achieve the above purpose, the present invention provides the following technical solutions: a fluorescent probe for synchronously quantifying a plurality of phosphorus-containing substances comprises three types of zinc sulfide quantum dots doped with different metal ions and different colors: znS: cu + -cyan-Fe 3+ 、ZnS:Cu + -yellow-Sn 2+ 、ZnS:Cu + -orange-Ni 2+ The three materials have no fluorescence under the irradiation of ultraviolet lamp, and respectively recover to cyan, yellow and orange red under the irradiation of ultraviolet lamp after reacting with PPi, pi, ATP.
Preferably, the zinc sulfide surface has carboxylic acid groups that provide binding sites for metal ion coordination.
Preferably, the preparation method of the fluorescent probe comprises the following specific steps:
(1) Weighing 1-10 mmol of zinc acetate and 0.01-0.1 mmol of copper sulfate pentahydrate, dissolving in 50-500 mL of ultrapure water, slowly adding 100-1000 mu L of 3-mercaptopropionic acid (MPA) after ultrasonic dissolution, and continuing ultrasonic dissolution of the mixed solution; adjusting the pH value to 7-11 by using 1-2 mol/L sodium hydroxide; taking 10-50 mL of mixed solution for hydrothermal reaction, setting the reaction temperature to be 100-200 ℃ and the reaction time to be three groups of a, b and c, wherein a is 10-50 min, b is 60-150 min, c is 160-250 min, cooling three groups of zinc sulfide quantum dots prepared in different hydrothermal time to room temperature, dialyzing for 1-2 days by using dialysis bags respectively, and obtaining ZnS: cu in group a + -Cyan, group b gives ZnS: cu + Yellow, group c gives ZnS: cu + -orange；
(2) Respectively doping three zinc sulfide quantum dots with different colors obtained in the step (1) with metal ions Fe 3+ 、Sn 2+ 、Ni 2+ Three fluorescent probes were obtained: znS: cu + -cyan-Fe 3+ ，ZnS:Cu + -yellow-Sn 2+ ，ZnS:Cu + -orange-Ni 2+ The method comprises the steps of carrying out a first treatment on the surface of the Wherein:
ZnS:Cu + -cyan-Fe 3+ the preparation process of the fluorescent probe comprises the following steps: znS: cu in an amount of 0.5 to 1mL + -cyan is mixed with 0.5-1 mL of ultra pure water in a 4mL cuvette, and then Fe is added 3+ In the mixed solution, fluorescence spectrum measurement is carried out on a fluorometer, and the optimal Fe is selected according to the fluorescence quenching condition 3+ Concentration;
ZnS:Cu + -yellow-Sn 2+ the preparation process of the fluorescent probe comprises the following steps: znS: cu in an amount of 0.5 to 1mL + Mixing yellow with 0.5-1 mL of ultra-pure water in a 4mL cuvette, and then adding Sn 2+ In the mixed solution, fluorescence spectrum measurement is carried out on a fluorometer, and the optimal Sn is selected according to the fluorescence quenching condition 2+ Concentration;
ZnS:Cu + -orange-Ni 2+ the preparation process of the fluorescent probe comprises the following steps: znS: cu in an amount of 0.5 to 1mL + -orange and 0.5-1 mL of ultrapure water in 4mL of colorimetric colorMixing in a dish, and then adding Ni 2+ In the mixed solution, fluorescence spectrum measurement is carried out on a fluorometer, and the optimal Ni is selected according to the fluorescence quenching condition 2+ Concentration.
Preferably, the fluorescent probe is applied to synchronous quantification of various phosphorus-containing substances, and comprises the following specific contents: using various PPi, pi, ATP solutions with known concentrations to respectively react with ZnS: cu + -cyan-Fe 3+ ，ZnS:Cu + -yellow-Sn 2+ ，ZnS:Cu + -orange-Ni 2+ The three fluorescent probes react, a fluorescent chart of the reaction solution under the irradiation of an ultraviolet lamp is detected after the reaction is sufficient, RGB values of the chart are analyzed, and relationship curves of PPi, pi, ATP concentration and the RGB values are obtained respectively; and adding the sample to be detected into the three fluorescent probe solutions, respectively detecting fluorescent patterns of the reaction solution under the irradiation of an ultraviolet lamp after the reaction is sufficient, analyzing RGB values of the patterns, and obtaining PPi, pi, ATP concentration in the sample to be detected according to the three relation curves.
Preferably, the fluorescent probe is applied to synchronous quantification of various phosphorus-containing substances, the excitation wavelength of an ultraviolet lamp is 365nm, the pH of a fluorescent probe solution is 7-11, and the reaction time is 30-100 minutes.
Preferably, the fluorescent probe is applied to synchronous quantification of a plurality of phosphorus-containing substances, and the part of the relationship curve with the straight line segment comprises: PPi concentration is 0.016. Mu.M-3. Mu.M, pi concentration is 0.0738. Mu.M-2. Mu.M, ATP concentration is 0.023. Mu.M-2. Mu.M.
Preferably, the fluorescent probe is applied to synchronous quantification of various phosphorus-containing substances, and RGB of pictures is obtained through photo analysis software PhotoMetrix.
Preferably, the fluorescent probe is applied to synchronous quantification of a plurality of phosphorus-containing substances, a plurality of PPi, pi, ATP solutions with known concentrations are respectively provided with a plurality of groups, and the average value of RGB is used as a detection result.
The device comprises a cartridge, wherein an ultraviolet lamp, a filter and a cuvette are arranged in the cartridge, the ultraviolet lamp irradiates the cuvette through the filter, a battery is also arranged in the cartridge for supplying power to the ultraviolet lamp, the cuvette is used for accommodating the fluorescent probe solution and adding a sample for reaction, and an opening is formed in the end part of the cartridge, which is positioned in the cuvette, for taking out the cuvette; a shooting hole is formed in a side wall of the cuvette, after the reaction is finished, a fluorescent picture of the solution in the cuvette under excitation of the ultraviolet lamp is shot through a mobile phone, a slot is formed in the side wall of the magazine where the shooting hole is formed, the mobile phone is inserted, and after the mobile phone is inserted, a mobile phone camera is opposite to the shooting hole.
Preferably, a storage module of the mobile phone is internally provided with photo analysis software PhotoMetrix for acquiring RGB values of fluorescent pictures so as to realize synchronous quantification of the fluorescent probes applied to various phosphorus-containing substances.
Compared with the prior art, the invention has the beneficial effects that:
the fluorescent probe for synchronously quantifying the multiple phosphorus-containing substances has the advantages of simple preparation method, convenient detection operation and high sensitivity, and the same method can detect three phosphorus-containing substances and has specificity; the detection device is convenient to carry and is beneficial to the field detection of various phosphorus-containing substances in actual samples.
Drawings
FIG. 1 is a transmission electron microscope image of zinc sulfide quantum dots prepared in example 1;
FIG. 2 shows the fluorescence change before and after the preparation of the fluorescent probe in example 1 and the detection of a phosphorus-containing material;
FIG. 3 is a graph showing ZnS: cu induced by PPi at various concentrations + -cyan-Fe 3+ Fluorescence change plot (a) and dotted plot (d); znS: cu caused by different concentrations of Pi + -yellow-Sn 2+ Fluorescence change plot (b) and dotted plot (e); znS: cu caused by ATP at different concentrations + -orange-Ni 2+ Fluorescence change plot (c) and dotted plot (f);
FIG. 4 is a graph of the corresponding fluorescence spectrum obtained by performing experiments with the three kinds of fluorescent probes obtained in example 1, in which (a) ZnS: cu + -yellow-Sn 2+ Adding Pi, PPi and ATP fluorescence spectrogram; (b) ZnS: cu + -cyan-Fe 3+ Adding Pi, PPi and ATP fluorescence spectrogram; (c) ZnS: cu + -orange-Ni 2+ Pi, PPi and ATP fluorescence spectra were added.
Detailed Description
A fluorescent probe for synchronously quantifying a plurality of phosphorus-containing substances comprises three types of zinc sulfide quantum dots doped with different metal ions and different colors: znS: cu + -cyan-Fe 3+ 、ZnS:Cu + -yellow-Sn 2+ 、ZnS:Cu + -orange-Ni 2+ The three materials have no fluorescence under the irradiation of ultraviolet lamp, and respectively recover to cyan, yellow and orange red under the irradiation of ultraviolet lamp after reacting with PPi, pi, ATP.
The zinc sulfide surface has rich carboxylic acid groups, and can provide rich binding sites for metal ion coordination.
The preparation method of the fluorescent probe comprises the following specific steps:
(1) Weighing 1-10 mmol of zinc acetate and 0.01-0.1 mmol of copper sulfate pentahydrate, dissolving in 50-500 mL of ultrapure water, slowly adding 100-1000 mu L of 3-mercaptopropionic acid (MPA) after ultrasonic dissolution, and continuing ultrasonic dissolution of the mixed solution; adjusting the pH value to 7-11 by using 1-2 mol/L sodium hydroxide; taking 10-50 mL of mixed solution for hydrothermal reaction, setting the reaction temperature to be 100-200 ℃ and the reaction time to be three groups of a, b and c, wherein a is 10-50 min, b is 60-150 min, c is 160-250 min, cooling three groups of zinc sulfide quantum dots prepared in different hydrothermal time to room temperature, dialyzing for 1-2 days by using dialysis bags respectively, and obtaining ZnS: cu in group a + -Cyan, group b gives ZnS: cu + Yellow, group c gives ZnS: cu + -orange；
(2) Respectively doping three zinc sulfide quantum dots with different colors obtained in the step (1) with metal ions Fe 3+ 、Sn 2+ 、Ni 2+ Three fluorescent probes were obtained: znS: cu + -cyan-Fe 3+ ，ZnS:Cu + -yellow-Sn 2+ ，ZnS:Cu + -orange-Ni 2+ The method comprises the steps of carrying out a first treatment on the surface of the Wherein:
ZnS:Cu + -cyan-Fe 3+ the preparation process of the fluorescent probe comprises the following steps: znS: cu in an amount of 0.5 to 1mL + -cyan is mixed with 0.5-1 mL of ultra pure water in a 4mL cuvette, and then Fe is added 3+ In the mixed solution, fluorescence spectrum measurement is carried out on a fluorometer, and the rootSelection of optimal Fe according to fluorescence quenching conditions 3+ Concentration;
ZnS:Cu + -yellow-Sn 2+ the preparation process of the fluorescent probe comprises the following steps: znS: cu in an amount of 0.5 to 1mL + Mixing yellow with 0.5-1 mL of ultra-pure water in a 4mL cuvette, and then adding Sn 2+ In the mixed solution, fluorescence spectrum measurement is carried out on a fluorometer, and the optimal Sn is selected according to the fluorescence quenching condition 2+ Concentration;
ZnS:Cu + -orange-Ni 2+ the preparation process of the fluorescent probe comprises the following steps: znS: cu in an amount of 0.5 to 1mL + Mixing the-orange with 0.5-1 mL of ultrapure water in a 4mL cuvette, and then adding Ni 2+ In the mixed solution, fluorescence spectrum measurement is carried out on a fluorometer, and the optimal Ni is selected according to the fluorescence quenching condition 2+ Concentration;
preferably, in the preparation process of the three fluorescent probes, the fluorescence quenching efficiency after doping is not lower than 70%.
The fluorescent probe is applied to synchronous quantification of various phosphorus-containing substances, and comprises the following specific contents: using various PPi, pi, ATP solutions with known concentrations to respectively react with ZnS: cu + -cyan-Fe 3+ ，ZnS:Cu + -yellow-Sn 2+ ，ZnS:Cu + -orange-Ni 2+ The three fluorescent probes react, a fluorescent chart of the reaction solution under the irradiation of an ultraviolet lamp is detected after the reaction is sufficient, RGB values of the chart are analyzed, and relationship curves of PPi, pi, ATP concentration and the RGB values are obtained respectively; and adding the sample to be detected into the three fluorescent probe solutions, respectively detecting fluorescent patterns of the reaction solution under the irradiation of an ultraviolet lamp after the reaction is sufficient, analyzing RGB values of the patterns, and obtaining PPi, pi, ATP concentration in the sample to be detected according to the three relation curves.
Furthermore, the fluorescent probe is applied to synchronous quantification of various phosphorus-containing substances, the excitation wavelength of an ultraviolet lamp is 365nm, the pH of a fluorescent probe solution is 7-11, and the reaction time is 30-100 minutes.
In addition, the fluorescent probe is applied to synchronous quantification of various phosphorus-containing substances, and the part of the relationship curve with the straight line segment comprises: PPi concentration is 0.016. Mu.M-3. Mu.M, pi concentration is 0.0738. Mu.M-2. Mu.M, ATP concentration is 0.023. Mu.M-2. Mu.M, and in these parts, more accurate quantification can be performed based on RGB values, whereas in other parts of the curve, the quantification accuracy is not as good as in the above-mentioned range because of the non-linear relationship.
Alternatively, the RGB of the picture can be obtained by photo metric, a picture analysis software.
In a preferred embodiment, the fluorescent probe is applied to synchronous quantification of a plurality of phosphorus-containing substances, wherein a plurality of sets of PPi, pi, ATP solutions with known concentrations are respectively provided, and the average value of RGB is used as a detection result.
In order to realize the synchronous quantification of the fluorescent probe applied to various phosphorus-containing substances, corresponding detection equipment is needed to realize the synchronous quantification, but the existing experimental equipment is usually larger and cannot meet the requirements of the invention, therefore, a device for synchronously quantifying various phosphorus-containing substances is designed, and the device comprises a cassette, wherein an ultraviolet lamp, a filter and a cuvette are arranged in the cassette, the ultraviolet lamp irradiates the cuvette through the filter, a battery is also arranged in the cassette for supplying power to the ultraviolet lamp, the cuvette is used for containing the fluorescent probe solution and adding a sample for reaction, and an opening is formed at the end part of the cassette, which is used for taking out the cuvette; a shooting hole is formed in a side wall of the cuvette, after the reaction is finished, a fluorescent picture of the solution in the cuvette under excitation of the ultraviolet lamp is shot through a mobile phone, a slot is formed in the side wall of the magazine where the shooting hole is formed, the mobile phone is inserted, and after the mobile phone is inserted, a mobile phone camera is opposite to the shooting hole.
The storage module of the mobile phone is internally provided with photo analysis software PhotoMetrix for acquiring RGB values of fluorescent pictures so as to realize the synchronous quantification of the fluorescent probe applied to various phosphorus-containing substances.
Example 1
Step 1: preparing multicolor doped zinc sulfide quantum dots:
firstly, weighing 6mmol of zinc acetate and 0.05mmol of copper sulfate pentahydrate, dissolving in 200mL of ultrapure water, and performing ultrasonic dissolution; secondly, slowly adding 800 mu L of 3-mercaptopropionic acid (MPA) into the mixed solution, and continuing ultrasonic dissolution of the mixed solution; thirdly, adjusting the pH value to 9 by using 2mol/L sodium hydroxide; finally, taking 20mL of the mixed solution for hydrothermal reaction, wherein the reaction temperature is as follows: 160 ℃, reaction time: 50. 120, 240min. After cooling the zinc sulfide quantum dots prepared in various different hydrothermal times to room temperature, dialyzing for 2 days by using 3500 dialysis bags, and obtaining three transmission electron microscope images of the zinc sulfide quantum dots shown in figure 1.
Step 2: designing and synthesizing a multicolor fluorescent probe:
several different colored zinc sulfide quantum dots (ZnS: cu) obtained by step 1 + -cyan，ZnS:Cu + -yellow and ZnS: cu + -orange) with metal ions (Fe) 3+ ，Sn 2+ ，Ni 2+ ) Three corresponding fluorescent probes are formed, namely ZnS: cu + -cyan-Fe 3+ ，ZnS:Cu + -yellow-Sn 2+ ，ZnS:Cu + -orange-Ni 2+ 。
ZnS:Cu + -cyan-Fe 3+ The preparation process of the fluorescent probe is as follows: 1mL ZnS: cu + -cyan is mixed with 1mL of ultrapure water in a 4mL cuvette, and then Fe is added 3+ Into the above mixed solution. Fluorescence spectrometry was performed on a fluorometer and ZnS: cu was selectively constructed based on fluorescence quenching + -cyan-Fe 3+ Optimal Fe of fluorescent probe 3+ Concentration; znS: cu + -yellow-Sn 2+ Fluorescent probe and ZnS: cu + -orange-Ni 2+ Is similar to ZnS: cu + -cyan-Fe 3+ The preparation process of the fluorescent probe;
as shown in fig. 2, the experiment resulted in: when 10 mu L of 30 mu M Fe 3+ Solution and 1mL ZnS: cu + 10. Mu.L of 8. Mu.M Sn when mixed with 1mL of ultrapure water 2+ When the solution was mixed with 1mL of ZnS: cu-yellow and 1mL of ultrapure water, 10. Mu.L of 10. Mu.M Ni 2+ When the solution is mixed with 1mL of ZnS: cu-orange and 1mL of ultrapure water, the fluorescence quenching efficiency of the three fluorescent probes is more than 75%.
Step 3: the detection process is as follows:
firstly, establishing a relation curve: using various PPi, pi, ATP solutions with known concentrations to respectively prepare ZnS: cu in the step 2 + -cyan-Fe 3+ ，ZnS:Cu + -yellow-Sn 2+ ，ZnS:Cu + -orange-Ni 2+ The three fluorescent probe solutions react, a fluorescent graph of the reaction solution under the irradiation of an ultraviolet lamp is detected after the reaction is sufficient, RGB values of the graph are analyzed to obtain a relation curve of PPi, pi, ATP concentration and RGB values, specifically, as shown in FIG. 3, PPi is 0-100 mu M, pi, PPi is 0-75 mu M, ATP, and a plurality of concentrations are 0-50 mu M, the corresponding fluorescent probes are used for detecting three phosphorus-containing substances respectively, the relation curve of RGB and concentration is shown as the graph of FIG. 3d, e and f, and the part of the relation curve which is approximate to a straight line segment and is obtained by the graph comprises: the concentration of PPi is 0.016 mu M-3 mu M, the concentration of Pi is 0.0738 mu M-2 mu M, the concentration of ATP is 0.023 mu M-2 mu M, and the reaction sensitivity of RGB values is high enough for different concentrations, so that the method can be applied to quantitative or semi-quantitative detection;
in this embodiment, a standard sample adding method is adopted, by respectively adding PPi, pi and ATP with different concentrations into deionized water, preparing a plurality of samples to be tested with known concentrations, adopting the synchronous quantitative device in the above embodiment, adding each sample to be tested into three cuvettes containing different fluorescent probe solutions respectively, after reacting for 60min, inserting the cuvettes into cuvette holes of a cassette, respectively detecting fluorescent patterns of reaction liquid under ultraviolet lamp irradiation, obtaining a series of fluorescent patterns by using a photographing function of a smart phone, obtaining RGB values of the patterns by using a photo analysis software photosmetrix in the smart phone, and obtaining PPi, pi, ATP concentration in the sample to be tested corresponding to three relationship curves (the establishment process of the relationship curves is preferably also carried out by adopting the synchronous quantitative device in the above embodiment, and the RGB of the analysis patterns is preferably also the same mobile phone and the same software to reduce detection errors);
the specific sample loading and test result data are shown in table 1 below:
table 1: recovery of experimental results in water samples
As can be seen from Table 1 above, the final observed recovery was between 100.7% and 104.4% for PPi at concentrations of 0.3, 0.9, 1.2, 1.4, 1.8. Mu.M, and between 98% and 101.5% for Pi at concentrations of 0.5, 0.9, 1.1, 1.4, 1.7. Mu.M in the five samples; ATP with the concentration of 0.5, 0.8, 1.3, 1.6 and 1.9 mu M has the recovery rate of the final measured result of 98 to 101.6 percent, and the accuracy of the detection result is high.
Example 2
Step 1: preparing multicolor doped zinc sulfide quantum dots:
firstly, 2mmol of zinc acetate and 0.04mmol of copper sulfate pentahydrate are weighed and dissolved in 100mL of ultrapure water, and dissolved by ultrasonic waves; secondly, slowly adding 500 mu L of 3-mercaptopropionic acid (MPA) into the mixed solution, and continuing ultrasonic dissolution of the mixed solution; thirdly, adjusting the pH value to 10 by using 2mol/L sodium hydroxide; finally, taking 20mL of the mixed solution for hydrothermal reaction, wherein the reaction temperature is as follows: 150 ℃, reaction time: 30. 90 min and 180min. After cooling the zinc sulfide quantum dots prepared in various different hydrothermal times to room temperature, dialyzing for 2 days by using 3500 dialysis bags.
Step 2: designing and synthesizing a multicolor fluorescent probe:
several different colored zinc sulfide quantum dots (ZnS: cu) obtained by step 1 + -cyan，ZnS:Cu + -yellow and ZnS: cu + -orange) with metal ions (Fe) 3+ ，Sn 2+ ，Ni 2+ ) Three corresponding fluorescent probes are formed, namely ZnS: cu + -cyan-Fe 3+ ，ZnS:Cu + -yellow-Sn 2+ ，ZnS:Cu + -orange-Ni 2+ 。
ZnS:Cu + -cyan-Fe 3+ The preparation process of the fluorescent probe is as follows: 1mL ZnS: cu + -cyan is mixed with 1mL of ultrapure water in a 4mL cuvette, and then Fe is added 3+ Into the above mixed solution. Fluorescence spectrometry was performed on a fluorometer and ZnS: cu was selectively constructed based on fluorescence quenching + -cyan-Fe 3+ Optimal Fe of fluorescent probe 3+ Concentration; znS: cu + -yellow-Sn 2+ Fluorescent probe and ZnS: cu + -orange-Ni 2+ Is made of (1)The preparation process is similar to ZnS: cu + -cyan-Fe 3+ The preparation process of the fluorescent probe;
the test results are: when 10 mu L of 40 mu M Fe 3+ Solution and 1mL ZnS: cu + 10. Mu.L of 9. Mu.M Sn when mixed with 1mL of ultrapure water 2+ When the solution was mixed with 1mL of ZnS: cu-yellow and 1mL of ultrapure water, 10. Mu.L of 15. Mu.M Ni 2+ When the solution is mixed with 1mL of ZnS: cu-orange and 1mL of ultrapure water, the fluorescence quenching efficiency of the three fluorescent probes is more than 75%.
The relation curve is established, the detection process is the same as that of the embodiment 1, the reaction time is only changed to 100 minutes, and the accuracy of the detection result is high.
Example 3
Step 1: preparing multicolor doped zinc sulfide quantum dots:
firstly, weighing 3mmol of zinc acetate and 0.06mmol of copper sulfate pentahydrate, dissolving in 150mL of ultrapure water, and performing ultrasonic dissolution; secondly, slowly adding 600 mu L of 3-mercaptopropionic acid (MPA) into the mixed solution, and continuing ultrasonic dissolution of the mixed solution; thirdly, adjusting the pH value to 10.5 by using 2mol/L sodium hydroxide; finally, taking 20mL of the mixed solution for hydrothermal reaction, wherein the reaction temperature is as follows: 140 ℃, reaction time: 40. 100 and 240min. After cooling the zinc sulfide quantum dots prepared in various different hydrothermal times to room temperature, dialyzing for 2 days by using 3500 dialysis bags.
Step 2: designing and synthesizing a multicolor fluorescent probe:
several different colored zinc sulfide quantum dots (ZnS: cu) obtained by step 1 + -cyan，ZnS:Cu + -yellow and ZnS: cu + -orange) with metal ions (Fe) 3+ ，Sn 2+ ，Ni 2+ ) Three corresponding fluorescent probes are formed, namely ZnS: cu + -cyan-Fe 3+ ，ZnS:Cu + -yellow-Sn 2+ ，ZnS:Cu + -orange-Ni 2+ 。
ZnS:Cu + -cyan-Fe 3+ The preparation process of the fluorescent probe is as follows: 1mL ZnS: cu + -cyan is mixed with 1mL of ultrapure water in a 4mL cuvette, and then Fe is added 3+ Into the above mixed solution. Performed on a fluorometerFluorescence spectrometry, and ZnS: cu is selectively constructed according to fluorescence quenching conditions + -cyan-Fe 3+ Optimal Fe of fluorescent probe 3+ Concentration; znS: cu + -yellow-Sn 2+ Fluorescent probe and ZnS: cu + -orange-Ni 2+ Is similar to ZnS: cu + -cyan-Fe 3+ The preparation process of the fluorescent probe;
the test results are: when 10 mu L of 70 mu M Fe 3+ Solution and 1mL ZnS: cu + 10. Mu.L of 15. Mu.M Sn when mixed with 1mL of ultrapure water 2+ When the solution was mixed with 1mL of ZnS: cu-yellow and 1mL of ultrapure water, 10. Mu.L of 25. Mu.M Ni 2+ When the solution is mixed with 1mL of ZnS: cu-orange and 1mL of ultrapure water, the fluorescence quenching efficiency of the three fluorescent probes is more than 70%.
The relation curve is established, the detection process is the same as that of the embodiment 1, the reaction time is changed to 50min, and the accuracy of the detection result is high.
Example 4
As shown in FIG. 4, experiments were performed with three kinds of fluorescent probes prepared in example 1, in ZnS: cu + -cyan-Fe 3+ Respectively adding Pi, PPi and ATP sample, reacting for 60min, and exciting with ultraviolet lamp with wavelength of 365nm to obtain fluorescence spectrum shown in figure 4b; in ZnS: cu + -yellow-Sn 2+ Respectively adding Pi, PPi and ATP sample, reacting for 60min, and exciting with ultraviolet lamp with wavelength of 365nm to obtain fluorescence spectrum shown in figure 4a; in ZnS: cu + -orange-Ni 2+ Respectively adding Pi, PPi and ATP sample, reacting for 60min, and exciting with ultraviolet lamp with wavelength of 365nm to obtain fluorescence spectrum chart shown in figure 4c; as can be seen, all three fluorescent probes were specific.
The above is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions easily contemplated by those skilled in the art within the technical scope of the present invention should be covered by the present invention. Therefore, the protection scope of the present invention should be defined by the claims.
The present invention is not described in detail in the present application, and is well known to those skilled in the art.
Claims (10)
1. The fluorescent probe for synchronously quantifying a plurality of phosphorus-containing substances is characterized by comprising three types of zinc sulfide quantum dots doped with different metal ions and different colors: znS: cu + -cyan-Fe 3+ 、ZnS:Cu + -yellow-Sn 2+ 、ZnS:Cu + -orange-Ni 2+ The three materials have no fluorescence under the irradiation of ultraviolet lamp, and respectively recover to cyan, yellow and orange red under the irradiation of ultraviolet lamp after reacting with PPi, pi, ATP.
2. The fluorescent probe of claim 1, wherein: the surface of the zinc sulfide is provided with carboxylic acid groups, and can provide binding sites for metal ion coordination.
3. The method for preparing a fluorescent probe according to claim 1 or 2, comprising the following specific steps:
(1) Weighing 1-10 mmol of zinc acetate and 0.01-0.1 mmol of copper sulfate pentahydrate, dissolving in 50-500 mL of ultrapure water, slowly adding 100-1000 mu L of 3-mercaptopropionic acid (MPA) after ultrasonic dissolution, and continuing ultrasonic dissolution of the mixed solution; adjusting the pH value to 7-11 by using 1-2 mol/L sodium hydroxide; taking 10-50 mL of mixed solution for hydrothermal reaction, setting the reaction temperature to be 100-200 ℃ and the reaction time to be three groups of a, b and c, wherein a is 10-50 min, b is 60-150 min, c is 160-250 min, cooling three groups of zinc sulfide quantum dots prepared in different hydrothermal time to room temperature, dialyzing for 1-2 days by using dialysis bags respectively, and obtaining ZnS: cu in group a + -Cyan, group b gives ZnS: cu + Yellow, group c gives ZnS: cu + -orange；
(2) Respectively doping three zinc sulfide quantum dots with different colors obtained in the step (1) with metal ions Fe 3+ 、Sn 2+ 、Ni 2+ Three fluorescent probes were obtained: znS: cu + -cyan-Fe 3+ ，ZnS:Cu + -yellow-Sn 2+ ，ZnS:Cu + -orange-Ni 2+ The method comprises the steps of carrying out a first treatment on the surface of the Wherein:
ZnS:Cu + -cyan-Fe 3+ the preparation process of the fluorescent probe comprises the following steps: znS: cu in an amount of 0.5 to 1mL + -cyan is mixed with 0.5-1 mL of ultra pure water in a 4mL cuvette, and then Fe is added 3+ In the mixed solution, fluorescence spectrum measurement is carried out on a fluorometer, and the optimal Fe is selected according to the fluorescence quenching condition 3+ Concentration;
ZnS:Cu + -yellow-Sn 2+ the preparation process of the fluorescent probe comprises the following steps: znS: cu in an amount of 0.5 to 1mL + Mixing yellow with 0.5-1 mL of ultra-pure water in a 4mL cuvette, and then adding Sn 2+ In the mixed solution, fluorescence spectrum measurement is carried out on a fluorometer, and the optimal Sn is selected according to the fluorescence quenching condition 2+ Concentration;
ZnS:Cu + -orange-Ni 2+ the preparation process of the fluorescent probe comprises the following steps: znS: cu in an amount of 0.5 to 1mL + Mixing the-orange with 0.5-1 mL of ultrapure water in a 4mL cuvette, and then adding Ni 2+ In the mixed solution, fluorescence spectrum measurement is carried out on a fluorometer, and the optimal Ni is selected according to the fluorescence quenching condition 2+ Concentration.
4. The fluorescent probe according to claim 1 or 2, wherein: the method is applied to synchronous quantification of various phosphorus-containing substances, and comprises the following specific contents: using various PPi, pi, ATP solutions with known concentrations to respectively react with ZnS: cu + -cyan-Fe 3+ ，ZnS:Cu + -yellow-Sn 2+ ，ZnS:Cu + -orange-Ni 2+ The three fluorescent probes react, a fluorescent chart of the reaction solution under the irradiation of an ultraviolet lamp is detected after the reaction is sufficient, RGB values of the chart are analyzed, and relationship curves of PPi, pi, ATP concentration and the RGB values are obtained respectively; and adding the sample to be detected into the three fluorescent probe solutions, respectively detecting fluorescent patterns of the reaction solution under the irradiation of an ultraviolet lamp after the reaction is sufficient, analyzing RGB values of the patterns, and obtaining PPi, pi, ATP concentration in the sample to be detected according to the three relation curves.
5. The fluorescent probe of claim 4, wherein: when the fluorescent probe solution is applied to synchronous quantification of various phosphorus-containing substances, the excitation wavelength of an ultraviolet lamp is 365nm, the pH of the fluorescent probe solution is 7-11, and the reaction time is 30-100 minutes.
6. The fluorescent probe of claim 4, wherein: when the method is applied to synchronous quantification of various phosphorus-containing substances, the part with the relationship curve being a straight line segment comprises the following steps: PPi concentration is 0.016. Mu.M-3. Mu.M, pi concentration is 0.0738. Mu.M-2. Mu.M, ATP concentration is 0.023. Mu.M-2. Mu.M.
7. The fluorescent probe of claim 4, wherein: when the method is applied to synchronous quantification of various phosphorus-containing substances, RGB of the picture is obtained through photo metric by picture analysis software.
8. The fluorescent probe of claim 4, wherein: when the method is applied to synchronous quantification of various phosphorus-containing substances, a plurality of PPi, pi, ATP solutions with known concentrations are respectively provided with a plurality of groups, and RGB average values are respectively used as detection results.
9. A device for synchronously quantifying a plurality of phosphorus-containing substances, which is characterized in that: the device comprises a cartridge, wherein an ultraviolet lamp, a filter and a cuvette are arranged in the cartridge, the ultraviolet lamp irradiates the cuvette through the filter, and a battery is also arranged in the cartridge for supplying power to the ultraviolet lamp; a cuvette for holding a solution of the fluorescent probe according to any one of claims 1, 2, 4 to 8 or a solution of the fluorescent probe prepared by the preparation method according to claim 3, and for adding a sample for reaction; the end part of the cartridge, which is positioned at the cuvette, is provided with an opening for taking out the cuvette; a shooting hole is formed in a side wall of the cuvette, after the reaction is finished, a fluorescent picture of the solution in the cuvette under excitation of the ultraviolet lamp is shot through a mobile phone, a slot is formed in the side wall of the magazine where the shooting hole is formed, the mobile phone is inserted, and after the mobile phone is inserted, a mobile phone camera is opposite to the shooting hole.
10. The device according to claim 9, wherein a storage module of the mobile phone stores photo analysis software photosystem for obtaining RGB values of fluorescent pictures, so as to realize synchronous quantification of the fluorescent probe according to any one of claims 4 to 8 applied to a plurality of phosphorus-containing substances.
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