NL2027546A - Method of producing an organoid - Google Patents

Method of producing an organoid

Info

Publication number
NL2027546A
NL2027546A NL2027546A NL2027546A NL2027546A NL 2027546 A NL2027546 A NL 2027546A NL 2027546 A NL2027546 A NL 2027546A NL 2027546 A NL2027546 A NL 2027546A NL 2027546 A NL2027546 A NL 2027546A
Authority
NL
Netherlands
Prior art keywords
organoid
producing
Prior art date
Application number
NL2027546A
Other versions
NL2027546B1 (en
Inventor
Caroline Rios Anne
Buchholz Maj-Britt
Florentia Dekkers Johanna
Original Assignee
Prinses Maxima Centrum Voor Kinderoncologie B V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prinses Maxima Centrum Voor Kinderoncologie B V filed Critical Prinses Maxima Centrum Voor Kinderoncologie B V
Priority to NL2027546A priority Critical patent/NL2027546B1/en
Priority to PCT/NL2022/050070 priority patent/WO2022173300A1/en
Priority to EP22705172.9A priority patent/EP4291636A1/en
Application granted granted Critical
Publication of NL2027546A publication Critical patent/NL2027546A/en
Publication of NL2027546B1 publication Critical patent/NL2027546B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0631—Mammary cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0695—Stem cells; Progenitor cells; Precursor cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/195—Heregulin, neu differentiation factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Dermatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Oncology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
NL2027546A 2021-02-11 2021-02-11 Method of producing an organoid NL2027546B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
NL2027546A NL2027546B1 (en) 2021-02-11 2021-02-11 Method of producing an organoid
PCT/NL2022/050070 WO2022173300A1 (en) 2021-02-11 2022-02-11 Method of producing an organoid
EP22705172.9A EP4291636A1 (en) 2021-02-11 2022-02-11 Method of producing an organoid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL2027546A NL2027546B1 (en) 2021-02-11 2021-02-11 Method of producing an organoid

Publications (2)

Publication Number Publication Date
NL2027546A true NL2027546A (en) 2022-09-12
NL2027546B1 NL2027546B1 (en) 2022-09-12

Family

ID=75954200

Family Applications (1)

Application Number Title Priority Date Filing Date
NL2027546A NL2027546B1 (en) 2021-02-11 2021-02-11 Method of producing an organoid

Country Status (3)

Country Link
EP (1) EP4291636A1 (en)
NL (1) NL2027546B1 (en)
WO (1) WO2022173300A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL311937A (en) * 2021-10-27 2024-06-01 Soci?T? Des Produits Nestl? S A Method for producing milk like products
CN115838690A (en) * 2023-02-24 2023-03-24 深圳明澳生物科技有限公司 Method for transfecting organoid by lentivirus and application

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080299540A1 (en) * 2004-05-07 2008-12-04 Whitehead Institute For Biomedical Research Hormone responsive tissue culture system and uses thereof
WO2016083612A1 (en) * 2014-11-27 2016-06-02 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for expanding breast epithelial stem cells
US20210062148A1 (en) * 2019-08-26 2021-03-04 Massachusetts Institute Of Technology Breast milk derived progenitor cells and cell systems

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080299540A1 (en) * 2004-05-07 2008-12-04 Whitehead Institute For Biomedical Research Hormone responsive tissue culture system and uses thereof
WO2016083612A1 (en) * 2014-11-27 2016-06-02 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for expanding breast epithelial stem cells
US20210062148A1 (en) * 2019-08-26 2021-03-04 Massachusetts Institute Of Technology Breast milk derived progenitor cells and cell systems

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
BROUTIER, L ET AL.: "Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation", NAT. PROTOC., vol. 11, 2016, pages 1724 - 1743, XP055534706, DOI: 10.1038/nprot.2016.097
CATTANEO, C. M. ET AL.: "Tumour organoid-T-cell coculture systems", NAT. PROTOC., vol. 15, 2020, pages 15 - 39, XP036977352, DOI: 10.1038/s41596-019-0232-9
DEKKERS JOHANNA F ET AL: "Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 16, no. 4, 10 March 2021 (2021-03-10), pages 1936 - 1965, XP037424459, ISSN: 1754-2189, [retrieved on 20210310], DOI: 10.1038/S41596-020-00474-1 *
DROST, J ET AL.: "Organoid culture systems for prostate epithelial and cancer tissue", NAT. PROTOC., vol. 11, 2016, pages 347 - 358, XP055589434, DOI: 10.1038/nprot.2016.006
DROST, J ET AL.: "Sequential cancer mutations in cultured human intestinal stem cells", NATURE, vol. 521, 2015, pages 43 - 47, XP055751106, DOI: 10.1038/nature14415
FUJII, M.MATANO, M.NANKI, K.SATO, T.: "Efficient genetic engineering of human intestinal organoids using electroporation", NAT. PROTOC., vol. 10, 2015, pages 1474 - 85, XP055576186, DOI: 10.1038/nprot.2015.088
RIOS, A.FU, N.LINDEMAN, G. ET AL.: "In situ identification of bipotent stem cells in the mammary gland", NATURE, vol. 506, 2014, pages 322 - 327, XP055240938, Retrieved from the Internet <URL:https://doi.org/10.1038/nature12948> DOI: 10.1038/nature12948
ROSENBLUTH JENNIFER M. ET AL: "Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages", NATURE COMMUNICATIONS, vol. 11, no. 1, 1 December 2020 (2020-12-01), pages 1 - 14, XP055848023, Retrieved from the Internet <URL:https://www.nature.com/articles/s41467-020-15548-7.pdf> DOI: 10.1038/s41467-020-15548-7 *
SACHS NORMAN ET AL: "A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity", CELL, ELSEVIER, AMSTERDAM NL, vol. 172, no. 1, 7 December 2017 (2017-12-07), pages 373, XP085336459, ISSN: 0092-8674, DOI: 10.1016/J.CELL.2017.11.010 *
SACHS NORMAN: "Table S2 Breast cancer organoid medium recipe Medium component Supplier Catalogue number R-Spondin 1 conditioned medium home made or R-Spondin 3 R&D 3500-RS", 7 December 2017 (2017-12-07), XP055848236, Retrieved from the Internet <URL:https://ars.els-cdn.com/content/image/1-s2.0-S0092867417313193-mmc2.xlsx> [retrieved on 20211006] *
SACHS, N ET AL.: "A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity", CELL, vol. 172, 2018, pages 373 - 386
SACHSNORMAN ET AL.: "A living biobank of breast cancer organoids captures disease heterogeneity", CELL, vol. 1-2, 2018, pages 373 - 386

Also Published As

Publication number Publication date
NL2027546B1 (en) 2022-09-12
EP4291636A1 (en) 2023-12-20
WO2022173300A1 (en) 2022-08-18

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