NL2026830B1 - Reaction device for absorbing molybdenum disulfide by chlorella - Google Patents

Reaction device for absorbing molybdenum disulfide by chlorella Download PDF

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Publication number
NL2026830B1
NL2026830B1 NL2026830A NL2026830A NL2026830B1 NL 2026830 B1 NL2026830 B1 NL 2026830B1 NL 2026830 A NL2026830 A NL 2026830A NL 2026830 A NL2026830 A NL 2026830A NL 2026830 B1 NL2026830 B1 NL 2026830B1
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chlorella
culture cylinder
air
mesh
aeration
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NL2026830A
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NL2026830B9 (en
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Feng Anqi
Li Tian
Zhou Qixing
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Univ Nankai
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/06Tubular
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Biotechnology (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The disclosure discloses a reaction device for absorbing molybdenum disulfide by chlorella, including a culture cylinder and supporting legs provided on four sides of the culture cylinder, where a placement cover is provided in the culture cylinder, pressing rings are provided on both sides of an inner wall of the placement cover, mesh plates are provided on inner walls of the pressing rings, an aeration rod is provided at the bottom of the culture cylinder, an aeration pump is provided on one side of the culture cylinder, and an output end of the aeration pump is communicated with the aeration rod through a hose. Air is discharged upwards through vent holes evenly formed in circumferentially and evenly arranged supporting tubes, so that the upward oxygen discharge range can be expanded, and air is discharged upwards in the form of an air bubble column. Under the influence of air floating, Mo ions released by lamellar T- SLM082 or H-SLM082 are brought to an upper side and are in contact with the chlorella in a mesh basin through flow holes and meshes of the mesh basin, and the chlorella in the mesh basin can uniformly receive the air and the Mo ions released by lamellar T-SLM082 or H- SLM082. This has a good experimental effect and solves the problem of poor experimental effects in the prior art.

Description

-1-
REACTION DEVICE FOR ABSORBING MOLYBDENUM DISULFIDE BY CHLORELLA
TECHNICAL FIELD The disclosure belongs to the technical field of environmental protection, and particularly relates to a reaction device for absorbing molybdenum disulfide by chlorella.
BACKGROUND SLMoS; has been used in optoelectronics, hydrogen evolution and medicine because of its excellent properties. SLMoS, with different phases shows different properties, such as conductivity and catalytic performance. With the production, processing and application, SLMoS: enters the ecological environment through water circulation, atmospheric circulation, etc. which brings potential risks to the environment and human beings. However, the research on its toxicity to aquatic organisms continues at present.
The research mainly focuses on the toxic effects of SLMoS; in different phases on chlorella. The phase change of SLMoS: is induced by microwave to prepare monolayer molybdenum disulfide dominated by 1T phase and monolayer molybdenum disulfide dominated by 2H phase, which is characterized by various electron microscopes, X-ray photoelectron spectrometers and electron spin resonance spectrometers. The results show that compared with 2H-SLMoS;, IT-SLMoS; has higher electronic conductivity and higher electron-hole separation efficiency, and 1T-SLMoS; releases more molybdenum ions into the water environment. Because Mo ions have toxic effects on chlorella, this also explains why 1T- SLMoS:; has more serious toxic effects on chlorella than 2H-SLMoS:. At present, most of reaction experiments are conducted outdoors, and there are some shortcomings: due to the changeable outdoor environment, the air supply to the chlorella and the contact amount between the chlorella and molybdenum disulfide are greatly affected by the external environment, and experimental results are poor.
SUMMARY The disclosure provides a reaction device for absorbing molybdenum disulfide by chlorella to solve the problems of poor experimental effects in the prior art.
To achieve the above purpose, the disclosure provides the following technical solutions: A reaction device for absorbing molybdenum disulfide by chlorella, including a culture cylinder and supporting legs provided on four sides of the culture cylinder, where a placement cover is provided in the culture cylinder, pressing rings are provided on both sides of an inner wall of the placement cover, mesh plates are provided on inner walls of the pressing rings, an aeration
“0.
rod is provided at the bottom of the culture cylinder, an aeration pump is provided on one side of the culture cylinder, and an output end of the aeration pump is communicated with the aeration rod through a hose.
Preferably, a baffle ring is provided on a middle side of an inner side of the placement cover, an outer wall of the pressing ring is provided with an external thread, the inner wall of the placement cover is provided with an internal thread, and the external thread is in threaded connection with the internal thread. A handle is provided at the top of the pressing ring on the upper side, supporting tubes are circumferentially and evenly provided below the pressing ring on the lower side, vent holes are evenly formed in an upper side of the supporting tube, a connecting tube is provided at the bottom of the supporting tube, the supporting tube is communicated with the connecting tube, and the aeration rod is located in the connecting tub. Preferably, a threaded cover 1s provided at the bottom of the connecting tube, a threaded tube is provided at a bottom end of the inside of the culture cylinder, and the threaded cover is in threaded connection with the threaded tube.
Preferably, the bottom of the culture cylinder is provided with a loading hole, an air inlet portion of the aeration rod passes through the loading hole, and filling mud is arranged in a matching gap between the loading hole and the air inlet portion of the aeration rod. Preferably, a cover ring is provided at the top of the culture cylinder, a medium plate is provided in the cover ring, flow holes are evenly formed in the medium plate, a mesh basin is provided on the medium plate. sliding holes are symmetrically formed in both sides of the cover ring, safety pins are inserted in the sliding holes, insertion grooves are symmetrically disposed at both sides of the culture cylinder, and the safety pins are inserted in the insertion grooves. Preferably, the insertion groove is sleeved with a spring, and both ends of the spring are connected to the cover ring and a head of the safety pin respectively. Compared with the prior art, the disclosure has the following beneficial effects: The reaction device for absorbing molybdenum disulfide by chlorella provided by the disclosure discharges air upwards through vent holes evenly formed in circumferentially and evenly arranged supporting tubes. In this way, the upward oxygen discharge range can be expanded, and air is discharged upwards in the form of an air bubble column. Under the influence of air floating, Mo ions released by lamellar T-SLMoS: or H-SLMoS; can be brought to an upper side and are in contact with the chlorella in a mesh basin through flow holes and meshes of the mesh basin. The chlorella in the mesh basin can uniformly receive the air and the Mo ions released by lamellar T-SLMoS:; or H-SLMoS:. This has a good experimental effect and solves the problem of poor experimental effects in the prior art.
The reaction device for absorbing molybdenum disulfide by chlorella provided by the
-3- disclosure, a cover ring, a pressing ring and a threaded cover are detachable, and a mesh basin and mesh plates can be dismantled from the culture cylinder, such that the mesh basin and the mesh plates can be cleaned and disinfected.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic front view of the disclosure: FIG. 2 is a partially cross-sectional schematic view of FIG. 1; FIG. 3 is a schematic view of an enlarged structure at a position a of FIG. 2; FIG. 4 is a schematic view of an enlarged structure at a position b of FIG. 2; FIG. 5 is a schematic view of an enlarged structure at a position c of FIG. 2; and FIG. 6 is a schematic top view of FIG. 1. In the figures: 1. culture cylinder, 2. supporting leg, 3. placement cover, 4. baffle ring, 5. pressing ring, 6. mesh plate, 7. external thread, 8. internal thread, 9. handle, 10. supporting tube, 11. vent hole, 12. connecting tube, 13. threaded cover, 14. threaded tube, 15. aeration rod, 16, loading hole, 17. filling mud, 18. aeration pump, 19. hose, 20. cover ring, 21. medium plate, 22. flow hole, 23. mesh basin, 24. sliding hole, 25. safety pin, 26. insertion groove, 27. spring.
DETAILED DESCRIPTION The following clearly and completely describes the technical solutions in the examples of the disclosure with reference to accompanying drawings in the examples of the disclosure. Apparently, the described examples are merely some rather than all of the examples of the disclosure. All other examples obtained by a person of ordinary skill in the art based on the examples of the disclosure without creative efforts shall fall within the protection scope of the disclosure. Referring to FIGs. 1, 2 and 3, a reaction device for absorbing molybdenum disulfide by chlorella includes a culture cylinder 1 and supporting legs 2 welded to four sides of an outer wall of the culture cylinder 1. The culture cylinder 1 has a cylindrical structure, and a placement cover 3 is provided in the culture cylinder 1. The placement cover 3 has a ring shape, and mesh plates 6 are provided on an inner wall of a pressing ring 5. The mesh plate 6 has a circular mesh plate structure, and meshes of the mesh plate 6 have a diameter of 1 mm. The pressing ring 5 and the mesh plate 6 are an integrated part formed by injection molding and made of ABS plastics. A baffle ring 4 is provided on a middle side of an inner wall of the placement cover 3. The baffle ring 4 has a ring structure. The placement cover 3 and the baffle ring 4 are an integrated part formed by injection molding and are made of ABS plastics. An outer wall of the pressing ring 5 is provided with an external thread 7, and the inner wall of the
-4- placement cover 3 is provided with an internal thread 8. The external thread 7 is in threaded connection with the internal thread 8. When the external thread 7 locks the internal thread 8. the baffle ring 4 presses the pressing ring 5, and lamellar molybdenum disulfide is placed between the mesh plates 6.
Referring to FIGs. 1. 2. 4 and 5, a handle 9 is provided at the top of the pressing ring 5 on the upper side. The handle 9 has a U-shaped rod structure. The pressing ring 5 on the upper side and the handle 9 are an integrated part formed by injection molding and made of ABS plastics. Supporting tubes 10 are circumferentially and evenly provided below the pressing ring 5 on the lower side. The supporting tubes 10 each have a circular tube structure. The supporting tube 10 and the pressing ring 5 on the lower side are an integrated part formed by injection molding and made of ABS plastics. Vent hole 11 are evenly formed in the upper side of the supporting tube 10. Air is discharged upwards through the vent holes 11 evenly formed in circumferentially and evenly arranged supporting tubes 10, so that the upward oxygen discharge range can be expanded. A connecting tube 12 is provided at the bottom of the supporting tube 10 and the supporting tube 10 is communicated with the connecting tube 12, and the supporting tube 10 and the connecting tube 12 are an integrated part formed by injection molding. A threaded cover 13 is provided at the bottom of the connecting tube 12. The threaded cover 13 and the connecting tube 12 are an integrated part formed by injection molding and made of ABS plastics. The threaded cover 13 is communicated with the connecting tube 12. The threaded cover 13 has a circular cover structure with an inner wall provided with an internal thread. A threaded tube 14 is provided at the center of a bottom end inside the culture cylinder 1. The culture cylinder 1 and the threaded tube 14 are an integrated part formed by casting. The threaded tube 14 has a circular tube structure with an outer wall provided with an external thread, and the threaded cover 13 is in threaded connection with the threaded tube 14. An aeration rod 15 is located in the connecting tube 12. The aeration rod 15 has a 20 cm model. A loading hole 16 is formed at the center of the bottom of the culture cylinder 1. The loading hole 16 has a conical round hole structure. An air inlet portion of the aeration rod 15 passes through the loading hole 16, and a matching gap between the loading hole 16 and the air inlet portion of the aeration rod 15 is closely filled with filling mud 17. The filling mud 17 is plastic mud. Through the filling with the filling mud 17, water in the culture cylinder 1 can be prevented from being discharged downwards through the matching gap between the aeration rod 15 and the loading hole 16. An aeration pump 18 is placed on a rear side of the culture cylinder 1, and the model of the aeration pump 18 is SB-748. An output end of the acration pump 18 is nested in a hose 19 through interference fit, and the other end of the hose 19 is nested in the air inlet portion of the aeration rod 15 through interference fit. The aeration pump 18 operates in a plugging in mode and can discharge external air into the
-5- connecting tube 12 through the aeration rod 13. Since the output end of the aeration pump 18 is provided with a check valve, the water in the culture cylinder 1 can be prevented from returning to the aeration pump 18.
Referring to FIGs. 1, 2 and 6, the top of the culture cylinder 1 is slidably inserted into a cover ring 20, and the cover ring 20 has a ring structure with a U-shaped cross section. A medium plate 21 is provided on a middle side of an inner wall of the cover ring 20. The cover ring 20 and the medium plate 21 are an integrated part formed by casting and made of stainless steel.
The medium plate 21 has a circular plate structure, and flow holes 22 are evenly formed in the medium plate 21. The flow holes 22 each have a circular hole structure. The flow hole 22 has an inner diameter of 1 cm. A mesh basin 23 is placed on the medium plate 21. The mesh basin 23 is a grid-shaped round basin. The edge of the mesh basin 23 is welded to the top of the cover ring 20. The mesh basin 23 has a mesh diameter of 0.4 mm. The mesh basin 23 is internally used for placing chlorella. Sliding holes 24 are symmetrically formed in a left side and a right side of the cover ring 20. The sliding holes 24 each have a circular hole structure.
Safety pins 25 are slidably inserted in the sliding holes 24, and insertion grooves 26 are symmetrically formed in a left side and a right side of an upper portion of an outer wall of the culture cylinder 1. The insertion grooves 26 each have a circular groove structure. The safety pins 25 are slidably inserted in the insertion grooves 26. and the insertion grooves 26 are sleeved with springs 27. The springs 27 are each of a tension-retraction type, and an initial tension-retraction force of the spring 27 is 12 N. Both ends of the spring 27 are welded to the cover ring 20 and a head of the safety pin 25 respectively. Through elastic support by the spring 27. the safety pin 25 is restricted from being separated from the insertion groove 26. The safety pin 25 is inserted in the insertion groove 26, so that the cover ring 20 is prevented from being separated from the culture cylinder 1 upwards.
In implementation, the whole reaction device can be placed indoors, and the safety pin 25 is pulled outwards such that the safety pin 25 is separated from the insertion groove 26. The safety pin 25 is pulled upwards, so that the cover ring 20 is separated from the culture cylinder I upwards. Then the handle 9 is rotated and the placement cover 3 is pressed, so that the external thread 7 on the upper side can be unscrewed from the intemal thread 8 on the upper side. The pressing ring 5 is separated from the placement cover 3 upwards, and lamellar 1T- SLMoS: or 2H-SLMoS: is placed on the lower mesh plate 7 until the lamellar 1T-SLMoS: or 2H-SLMoS: approaches an upper surface of the baffle ring 4. In this case, the external thread 7 is re-screwed into the internal thread 8, then the cover ring 20 is re-nested into the top of the culture cylinder 1, and the safety pin 25 is re-inserted into the insertion groove 26. Through the clastic support by the spring 27, the safety pin 25 is restricted from being separated from the insertion groove 26. The safety pin 25 is inserted in the insertion groove 26, so that the cover
-6- ring 20 is prevented from being separated from the culture cylinder 1. Chlorella can be placed into the mesh basin 23 and spread flat in the mesh basin 23. Then water is added into the culture cylinder 1 until the chlorella can effectively receive water. The aeration pump 18 operates in a plugging in mode and can discharge external air into the connecting tube 12 through the aeration rod 15. The air is discharged upwards through the vent holes 11 evenly formed in circumferentially and evenly arranged supporting tubes 10, so that the upward oxygen discharge range can be expanded, and the air is discharged upwards in the form of an air bubble column and enters the lamellar 1 T-SLMoS; or 2H-SLMoS; through the mesh plate
6. Under the influence of air floating, Mo ions released by the lamellar IT-SLMoS; or 2H- SLMoS; can be brought to the upper side and are in contact with the chlorella in the mesh basin 23 through the flow holes 22 and meshes of the mesh basin 23, and the lamellar 1T- SLMoS: or 2H-SLMoS: can be selectively experimented first. In the process, the chlorella in the mesh basin 23 can evenly receive air and the Mo ions released by the lamellar 1T-SLMoS; or 2H-SLMoS:, and the experimental effect is good. Besides, the cover ring 20, the pressing ring 5 and the threaded cover 13 are detachable, and the mesh basin 23 and the mesh plates 6 can be dismantled from the culture cylinder 1, such that the mesh basin and the mesh plates can be cleaned and disinfected. Although examples of the disclosure have been shown and described, it can be understood by those of ordinary skill in the art that various changes, modifications, replacements and variations can be made to these examples without departing from the principles and spirit of the disclosure. The scope of the disclosure is defined by the appended claims and equivalents thereof.
NL2026830A 2020-04-23 2020-11-05 Reaction device for absorbing molybdenum disulfide by chlorella NL2026830B9 (en)

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CN202010328181.6A CN111575166B (en) 2020-04-23 2020-04-23 Utilize chlorella to absorb molybdenum disulfide reaction unit

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NL2026830B9 NL2026830B9 (en) 2022-03-30

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CN112273949A (en) * 2020-10-27 2021-01-29 威海职业学院 Mechanical design product design demonstration system

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WO2010129278A1 (en) * 2009-05-06 2010-11-11 Bioprocessh20 Llc Systems, apparatuses and methods for cultivating microorganisms and mitigation of gases
CN105238681A (en) * 2015-10-20 2016-01-13 王万森 Device utilizing chlorella to absorb carbon dioxide in biogas for purifying biogas
US20160166985A1 (en) * 2014-12-16 2016-06-16 Nano And Advanced Materials Institute Limited Photobioreactor system for air purification by using microalgae

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KR101999721B1 (en) * 2017-03-13 2019-07-12 인하대학교 산학협력단 Apparatus for harvesting microalgae
CN207108986U (en) * 2017-08-10 2018-03-16 华中科技大学 A kind of microalgae bactogen for preventing microalgae adherence
CN208776708U (en) * 2018-08-09 2019-04-23 济南大学 A kind of microalgae sterile culture device suitable for mixotrophic cultivation
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WO2010129278A1 (en) * 2009-05-06 2010-11-11 Bioprocessh20 Llc Systems, apparatuses and methods for cultivating microorganisms and mitigation of gases
US20160166985A1 (en) * 2014-12-16 2016-06-16 Nano And Advanced Materials Institute Limited Photobioreactor system for air purification by using microalgae
CN105238681A (en) * 2015-10-20 2016-01-13 王万森 Device utilizing chlorella to absorb carbon dioxide in biogas for purifying biogas

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