NL2026316B1 - Method for evaluating influences of toxic substances on mass transfer performance of activated sludge - Google Patents
Method for evaluating influences of toxic substances on mass transfer performance of activated sludge Download PDFInfo
- Publication number
- NL2026316B1 NL2026316B1 NL2026316A NL2026316A NL2026316B1 NL 2026316 B1 NL2026316 B1 NL 2026316B1 NL 2026316 A NL2026316 A NL 2026316A NL 2026316 A NL2026316 A NL 2026316A NL 2026316 B1 NL2026316 B1 NL 2026316B1
- Authority
- NL
- Netherlands
- Prior art keywords
- activated sludge
- mixed solution
- stained
- mass transfer
- transfer performance
- Prior art date
Links
- 239000010802 sludge Substances 0.000 title abstract description 135
- 238000012546 transfer Methods 0.000 title abstract description 58
- 238000000034 method Methods 0.000 title abstract description 40
- 239000003440 toxic substance Substances 0.000 title abstract description 35
- 231100000614 poison Toxicity 0.000 title abstract description 34
- 239000011259 mixed solution Substances 0.000 abstract description 57
- 238000012360 testing method Methods 0.000 abstract description 29
- 239000002351 wastewater Substances 0.000 abstract description 28
- 230000000694 effects Effects 0.000 abstract description 12
- 230000008569 process Effects 0.000 abstract description 12
- 230000000813 microbial effect Effects 0.000 abstract description 11
- 238000005273 aeration Methods 0.000 abstract description 10
- 238000011156 evaluation Methods 0.000 abstract description 6
- 239000000975 dye Substances 0.000 description 34
- 230000005764 inhibitory process Effects 0.000 description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 16
- 229910052760 oxygen Inorganic materials 0.000 description 16
- 239000001301 oxygen Substances 0.000 description 16
- 239000011159 matrix material Substances 0.000 description 15
- 239000011521 glass Substances 0.000 description 14
- 238000004364 calculation method Methods 0.000 description 13
- 230000035764 nutrition Effects 0.000 description 13
- 235000016709 nutrition Nutrition 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 239000000243 solution Substances 0.000 description 8
- 238000010186 staining Methods 0.000 description 8
- 238000003384 imaging method Methods 0.000 description 7
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 6
- 238000012512 characterization method Methods 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 231100000331 toxic Toxicity 0.000 description 5
- 230000002588 toxic effect Effects 0.000 description 5
- 239000006059 cover glass Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 229920000122 acrylonitrile butadiene styrene Polymers 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 239000004744 fabric Substances 0.000 description 3
- 229910021642 ultra pure water Inorganic materials 0.000 description 3
- 239000012498 ultrapure water Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 238000012757 fluorescence staining Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000011496 digital image analysis Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000003264 microelectrode measurement Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention discloses a method for evaluating influences of toxic substances on mass transfer performance of activated sludge. The method includes the following steps: (1) preparing an activated sludge mixed solution in a test group; (2) performing aerobic aeration on the test group, and transferring an appropriate amount of the activated sludge mixed solution as a to-be-stained activated sludge mixed solution; (3) adding an appropriate amount of standard stained mixed solution into the to-be-stained activated sludge mixed solution so as to form a stained activated sludge mixed solution, wherein a dye l in the standard stained mixed solution can enter microbial intact cells to present a fluorescent colour I, and a dye II can enter microbial damaged cells to present a fluorescent colour II; and (4) acquiring fluorescence intensity and fluorescent area of the stained activated sludge, wherein the mass transfer performance is characterized by fluorescence intensity OD of the fluorescent colour I per unit area. The present invention is efficient, simple and easy to operate, intuitive in result and low in technical requirement. A ratio of the toxic substances or wastewater containing the toxic substances to the activated sludge is controlled by the evaluation method, thereby ensuring that the activated sludge process achieves an excellent effect.
Description
TRANSFER PERFORMANCE OF ACTIVATED SLUDGE Technical Field The present invention relates to the field of water treatment, and particularly relates to a method for evaluating influences of toxic substances on mass transfer performance of activated sludge and an inhibition ratio of the mass transfer performance.
Background Petrochemical industry is a pillar industry and the lifeblood of economy in China. Petrochemical wastewater has the characteristics of high water yield, complicated pollutant composition and extensive toxic species. For a long time, lots of resources and energy are consumed during treatment of the petrochemical wastewater, but the petrochemical wastewater is still an important source of water environmental pollutants. Comprehensive treatment taking biological treatment as the core is a mainstream process of organic wastewater treatment, but the biological treatment is just easily affected by interference and impact of various toxic substances in the petrochemical wastewater and becomes a critical bottleneck that restricts industrial wastewater treatment efficiency for a long time. Therefore, building perfect biological treatment toxicity evaluation indexes to comprehensively evaluate the influences of biological treatment is a basic precondition and necessary means of efficient treatment of the petrochemical wastewater.
In a biological treatment toxicity index system, a mass transfer characteristic index is one of the most important indicators characterizing matrix transfer and oxygen transfer in a process of degrading organic matters by activated sludge, while mass transfer performance serving as a characterization parameter of the mass transfer characteristic index is capable of directly quantifying transfer conditions of the matrix and oxygen on the surface and interior of the activated sludge, thereby confirming the effects of the wastewater and components thereof for wrapping the activated sludge and hindering the mass transfer. With respect to wastewater containing a certain dose of oil or insoluble macromolecular organic matters and other toxic substances, such as refinery wastewater and ABS resin wastewater, in the biological treatment process, the toxic substances will be adsorbed and wrapped on the surface of the activated sludge to decrease the mass transfer performance, thereby influencing degradation of the activated sludge on the pollutants in water.
At present, a theoretical formula of Fick's laws is often adopted during evaluation and calculation of the mass transfer performance and an inhibition ratio thereof. In combination with microelectrode measurement and microscopic examination, theoretical model derivation calculation is performed to obtain the mass transfer performance and the inhibition ratio. Such a method is relatively high in theoretical technical level requirement of enterprise technicians, high in testing cost, cumbersome in operation and difficult for practical popularization and application. Therefore, it is very important to provide an efficient and convenient method for evaluating the mass transfer performance of the activated sludge and the inhibition ratio of the mass transfer performance.
Summary In order to solve a problem that an existing mass transfer performance calculation process is relatively high in theoretical technical level requirements for enterprise technicians, high in testing cost, cumbersome in operation and difficult for practical popularization and application, the purpose of the present invention is to provide a method for evaluating influences of toxic substances on mass transfer performance of activated sludge and an inhibition ratio of the mass transfer performance so as to estimate the influences of the toxic substances on the mass transfer performance of the activated sludge. The method achieves an effect of guiding treatment of the toxic substances or toxic wastewater by an activated sludge process. The method is efficient, simple and easy to operate, intuitive in result and low in technical requirement. A ratio of the toxic substances or wastewater containing the toxic substances to the activated sludge is controlled by the evaluation method, thereby ensuring that the activated sludge process achieves an excellent effect.
In a first aspect, the present invention provides a method for evaluating influences of toxic substances on mass transfer performance of activated sludge, including the following steps: (1) adding a nutrition matrix, activated sludge and toxic substances or a tested wastewater sample containing toxic substances into a container to form an activated sludge mixed solution, and defining the container as a test group; (2) performing aerobic aeration on the test group until a dissolved oxygen concentration is constant, and transferring a part of the activated sludge mixed solution as a to-be-stained activated sludge mixed solution; (3) adding a standard stained mixed solution into the to-be-stained activated sludge mixed solution, shaking up and standing, staining under a shade condition, and forming a stained activated sludge mixed solution, wherein the standard stained mixed solution is prepared by mixing and diluting a dye | and a dye Il under the shade condition; the dye | can enter microbial intact cells to present a fluorescent colour |, and the dye II can enter microbial damaged cells to present a fluorescent colour II; and (4) transferring a part of the stained activated sludge from the stained activated sludge mixed solution, and acquiring fluorescence intensity and fluorescent area of the stained activated sludge, wherein the mass transfer performance is characterized by fluorescence intensity OD of the fluorescent colour | per unit area. A calculating formula is as follows: OD = IntDen/Area (1-1)
wherein IntDen is the sum of fluorescence intensity of the fluorescent colour | in an activated sludge range, and Area is the sum of areas of fluorescent activated sludge of the fluorescent colour | and fluorescent activated sludge of the fluorescent colour II.
Preferably, in the step (4), a method for acquiring the fluorescence intensity and the fluorescent area includes: transferring an appropriate amount of the stained activated sludge to observe under a microscope, enabling the activated sludge to image clearly, and enabling a lens of the microscope to align at a corresponding fluorescent channel; and photographing by a camera, enabling photographing areas to not overlap, and keeping photographing until imaging of the activated sludge is totally photographed, wherein the quantity of photos in each group is not less than 30. IntDen is the sum of fluorescence intensity of the fluorescent colour | in the activated sludge; Area is the sum of areas of the fluorescent activated sludge in each photo; and OD is fluorescence intensity of the fluorescent colour | per unit area in each photo.
Further preferably, a method for photographing by a camera includes steps: dividing the stained activated sludge area into at least 30 areas, and photographing in sequence according to a sequence of the areas by the camera.
Further preferably, conditions that the mass transfer performance is characterized by the fluorescence intensity OD of the fluorescent colour | per unit area are as follows: relative standard deviation of the OD is less than 5%, and the area of the fluorescent activated sludge of the fluorescent colour Il is less than 5% of the total area of the fluorescent activated sludge; and then, data reproducibility is good, and activity of the activated sludge is excellent.
In a second aspect, the present invention provides a method for evaluating influences of toxic substances on an inhibition ratio of mass transfer performance of activated sludge, which is based on any of the above methods for evaluating influences of the toxic substances on the mass transfer performance of activated sludge, specifically: In the step (1), only a nutrition matrix and activated sludge are added into another container to form an activated sludge mixed solution, and the container is defined as a blank group; and the overall system of the blank group and the test group is kept consistent; in the step (2), aerobic aeration is performed in the two systems until the dissolved oxygen concentration is constant, and one part of the activated sludge mixed solution is respectively transferred from the two systems so as to serve as a to-be-stained activated sludge mixed solution; in the step (3), a standard stained mixed solution is respectively added into the two to-be- stained activated sludge mixed solutions, shaking up and standing are performed under a shade condition, and a stained activated sludge mixed solution is formed; in the step (4), a part of the stained activated sludge is respectively transferred from the stained activated sludge mixed solution, and the fluorescence intensity and fluorescent areas of the stained activated sludge in the two systems are acquired. A calculating formula of an inhibition ratio | of the mass transfer performance is as follows:
I=[1-ODs /ODg]x 100% (1-2) wherein ODs is fluorescence intensity of the fluorescent colour | per unit area in the test group, and ODs is fluorescence intensity of the fluorescent colour | per unit area in the blank group.
Further, in the step (4), the fluorescence intensity of the activated sludge in the test group and the blank group is not lower than 0.1. In the whole detection process, red fluorescence areas are all lower than 5%, thereby ensuring available biomass of the activated sludge.
The method for evaluating the mass transfer performance of the activated sludge provided in the present invention has beneficial effects as follows: (1) the method is accurate and efficient, the fluorescence intensity can be directly calculated so as to characterize the mass transfer performance by the fluorescence intensity, a conventional mass transfer rate parameter is replaced, a complicated calculation process is not needed, and the method is accurate in calculation and small in error; (2) the operation is simple and convenient, only fluorescence staining is adopted, the fluorescence intensity and the fluorescent area are acquired, and the operation can be completed by virtue of simple calculation; (3) the result is intuitive, that is, the mass transfer condition can be intuitively characterized by utilizing the fluorescence intensity of the activated sludge; and (4) the technical requirement is low, and engineered actual popularization is facilitated. Description of Drawings Fig. 1 is an operation flow chart when influences of toxic substances on mass transfer performance of activated sludge is evaluated. In the figure, 1: activated sludge; 2: nutrition matrix; 3: toxic substances or tested wastewater sample containing toxic substances; 4: glass plate; 5: dissolved oxygen tester; 8: pipette; 7: air compressor, 8: to-be-stained activated sludge mixed solution; 9: standard stained mixed solution; 10: microsyringe; 11: dropper; 12: glass slide; 13: fluorescence microscope; 14: CCD camera; and 15: computer. Detailed Description To make technical solutions of the present invention more clear, the present invention is further described in detail below in combination with specific drawings and specific embodiments. It should be indicated that, embodiments in the present invention and features in the embodiments can be combined with one another without conflict. A method for evaluating influences of toxic substances on mass transfer performance of activated sludge provided in the present invention, as shown in Fig. 1, includes the following steps:
(1) adding a nutrition matrix, activated sludge and toxic substances or a tested wastewater sample containing toxic substances into a beaker to form an activated sludge mixed solution, and defining the beaker as a test group; (2) performing aerobic aeration on the test group system by using an air compressor, 5 detecting a dissolved oxygen concentration in the system by using a dissolved oxygen tester until the dissolved oxygen concentration is constant; and transferring an appropriate amount of the activated sludge mixed solution from the test group system by a pipette; and adding the activated sludge mixed solution into a centrifuge tube for staining as a to-be-stained activated sludge mixed solution;
(3) adding an appropriate amount of standard stained mixed solution into the to-be-stained activated sludge mixed solution by a microsyringe, shaking up and standing, staining under shade cloth for 10-20 min, and forming a stained activated sludge mixed solution, wherein a preparation method of the standard stained mixed solution includes the steps: taking a dye | of a fluorescent colour | capable of entering microbial intact cells and a dye II of a fluorescent colour II capable of entering microbial damaged cells by using the microsyringe; mixing and diluting the dye | and the dye Il to an appropriate concentration with pure water in the shade cloth; and taking the solution as the standard stained mixed solution; and
(4) transferring an appropriate amount of the stained activated sludge from the stained activated sludge mixed solution onto a glass slide by a dropper; slightly covering the glass slide;
enabling the activated sludge to image clearly by adjusting a coarse/fine adjustment spiral of a microscope; adjusting a lens of the microscope to a corresponding fluorescent channel; installing a CCD camera on the microscope; connecting the CCD camera with a computer via a data cable; installing a CCD camera client on the computer, and enabling imaging of the microscope to be displayed on a computer screen in real time; manually adjusting a rotary knob to move the observed sample, and completing photographing, wherein the quantity of photos in each group is not less than 10, preferably 30; keeping photographing until imaging of the activated sludge is totally photographed, and ensuring that photographing areas do not overlap so as not to increase a calculation error of fluorescence intensity, thereby acquiring the fluorescence intensity and fluorescent area of the stained activated sludge mixed solution; and finally, performing fluorescence intensity and fluorescent area analysis on the photos, wherein the mass transfer performance is characterized by fluorescence intensity OD of the fluorescent colour | per unit area.
A calculating formula is as follows:
OD = IntDen/Area (1-1) In the formula, IntDen is the sum of fluorescence intensity of the fluorescent colour | in the activated sludge (the unit is A.U); and Area is the sum of areas of the fluorescent activated sludge of the fluorescent colour | and the fluorescent activated sludge of the fluorescent colour Il (the unit may be mm?2).
In the above evaluation method, IntDen is the sum of the fluorescence intensity of the fluorescent colour | in the activated sludge in each photo; Area is the sum of the areas of the fluorescent activated sludge in each photo; and OD is the fluorescence intensity of the fluorescent colour | per unit area in each photo. Through the above evaluation method, relative standard deviation of the OD in each group is less than 5%, which indicates that data reproducibility is good; and the area of the fluorescent activated sludge of the fluorescent colour Il is less than 5% of the total area of the fluorescent activated sludge, which indicates that activity of the activated sludge is excellent. The fluorescence intensity OD may serve as a characterization result of the mass transfer performance. According to the result, it may guide the judgment that an appropriate ratio of the toxic substances to the activated sludge and other relevant parameters are selected while treating the toxic substances or wastewater containing toxic substances by an activated sludge process, thereby meeting actual treatment requirements.
The present invention further provides a method for evaluating influences of toxic substances on an inhibition ratio of mass transfer performance of activated sludge. The method is based on the method for evaluating the influence of the toxic substances on the mass transfer performance of activated sludge. Operations in a blank group are performed according to an operating method in Fig. 1. The method specifically includes the following steps: In the step (1), only a nutrition matrix and activated sludge are added into another beaker to form an activated sludge mixed solution; any toxic wastewater or toxic substance is not added; the mixed solution is uniformly stirred by a glass rod and considered as a blank group; and the overall system of the blank group and the test group is kept consistent; In the step (2), aerobic aeration is performed in the two systems by using an air compressor; a dissolved oxygen concentration in the two systems is detected in real time by using a dissolved oxygen tester until the dissolved oxygen concentration is constant; and an appropriate amount of the activated sludge mixed solution is respectively transferred from the two systems so as to serve as a to-be-stained activated sludge mixed solution; In the step (3), an appropriate amount of a standard stained mixed solution is respectively added into the two to-be-stained activated sludge mixed solutions, shaking up and standing are performed; staining is performed a shade condition; and a stained activated sludge mixed solution is formed; In the step (4), an appropriate amount of the stained activated sludge is transferred, and the fluorescence intensity and fluorescent areas of the stained activated sludge in the two systems are acquired. A calculating formula of an inhibition ratio | of the mass transfer performance is as follows: I=[1-ODs /ODg]x 100% (1-2) In the formula, ODs is fluorescence intensity of the tested sample of the fluorescent colour | per unit area in the test group, and ODs is fluorescence intensity of the fluorescent colour | per unit area in the blank group.
Through the method for evaluating the mass transfer performance of the activated sludge and the inhibition ratio of the mass transfer performance provided by the present invention, and detection of water system in the system, it may be determined that, effluent of a sewage treatment plant is not up to standard at which inhibition ratio of the mass transfer performance. With respect to different kinds of wastewater, activated sludge and the like, the inhibition ratios of the mass transfer performance are different, and a certain effect of guiding the actual production process is achieved, thereby realizing industrialization.
In the method for evaluating the influences of the toxic substances on the mass transfer performance of the activated sludge and the inhibition ratio of the mass transfer performance, as a preferred embodiment, in the step (1), a ratio of the nutrition matrix to the activated sludge is 1:1-1:5 so as to ensure an adequate nutrition matrix in the reaction system. The nutrition is enriched in nutrient substances essential for microbial growth, such as carbon sources, nitrogen sources, inorganic salts and the like.
As a preferred embodiment, in the step (2), in the aerobic aeration process, aeration time is 15-25 min, preferably 20 min; the dissolved oxygen concentration is 2.5-4.0 mg/L, so that sufficient dissolved oxygen is provided for aerobic activated sludge, and the activated sludge fully contacts with the matrix; and a concentration of mixed liquor suspended solids (MLSS) in the activated sludge mixed solution is not lower than 1.0 g/L so as to ensure an effective sludge quantity in the reaction system.
As a preferred embodiment, in the step (3), a volume ratio of the dye | to the dye Il is (0.5-
1.5):1, further preferably 1:1; and a dilution volume ratio of the dyes to water is 1:30-1:3000 so as to fully dilute and mix the dyes.
In the step (3), the dye | is used for labeling intact cells so as to detect integrity of the cells; the dye II is used for labeling damaged cells so as to detect a death amount of the cells.
Specifically, the dye | and the dye II may be selected from cell imaging reagents for cell analysis produced by Thermo FisherScientific Company. For example, the dye | may be ActinGreen ™488 ReadyProbes® Reagent and can enter microbial intact cells to present green fluorescence; and the dye Il may be NucRed ™ Dead 647ReadyProbes® Reagent and can enter microbial damaged cells to present red fluorescence. Meanwhile, the lens of the microscope is adjusted to a channel WB and a channel WG. The dye | and the dye II need to be preserved under -30°C and can be preserved in a refrigerator.
As a preferred embodiment, in the step (3), a volume ratio of the standard stained mixed solution to the to-be-stained activated sludge mixed solution is 1:100-1:1000; and in order to enable the activated sludge to be uniformly stained, staining time under the shade cloth is 10-20 min, preferably 15 min.
As a preferred embodiment, in the step (4), a method for photographing by the CCD camera is as follows: the stained activated sludge area is divided into at least 30 areas; during specific operations, cover glass can be subjected to meshing in advance, or characterization of sludge is observed by naked eyes; photographing is performed according to experience; and photographing is performed according to a sequence, such as, from left to right and from top to bottom.
As a preferred embodiment, in the step (4), the photos can be subjected to fluorescence intensity and fluorescent area analysis by utilizing Image software. During calculation of the fluorescence intensity, the activated sludge presenting the fluorescent colour | should be totally covered during rendering markup, so as to avoid a calculation error of the fluorescence intensity.
As a preferred embodiment, the activated sludge in the test group and the blank group is selected from sludge at the end of an aerobic zone of biological treatment of a sewage plant; and in the whole detection process, the fluorescent area of the fluorescent colour II (that is, the death amount of the cells) is lower than 5% of the total area, so as to ensure effective biomass of the activated sludge.
As a preferred embodiment, the fluorescence intensity of the activated sludge (including the fluorescent colour | and the fluorescent colour Il) is not lower than 0.1, so as to ensure the activity of the activated sludge.
The method in the present invention has advantages as follows: firstly, the method is accurate and efficient, that is, the fluorescence intensity is directly calculated to characterize the mass transfer performance by utilizing the computer image analysis technology, the calculation is accurate, and the error is small; secondly, the operation is simple and convenient, that is, the operation may be completed by three steps, such as fluorescence staining, microscope photographing and image processing; thirdly, the result is intuitive, that is, the mass transfer condition may be intuitively characterized by utilizing the fluorescence intensity of the activated sludge; and fourthly, the technical requirement is low, and engineered actual popularization is facilitated.
The mass transfer performance of the toxic substances or wastewater containing toxic substances and the inhibition ratio of the mass transfer performance are actually characterized below by virtue of specific embodiments. The dye | can enter the microbial intact cells to present green fluorescence, and the dye Il can enter the microbial damaged cells to present red fluorescence.
Embodiment | A test sample was a characteristic toxic organic matter cyclohexane in refinery wastewater. In view of an actual concentration of the cyclohexane in the refinery wastewater, an addition concentration of the cyclohexane in the whole system was selected as 0-10.0 g/L. The cyclohexane was mixed and contacted with 300 mL of activated sludge and 400 mL of a nutrition matrix (having a volume ratio of 1:3), and pure water was supplemented, so that the reaction system was 900 mL. A concentration of mixed liquor suspended solids (MLSS) in a test group system was about 1.2 g/L, and the solution was uniformly stirred by a glass rod. After dissolved oxygen was subjected to aerobic aeration and full contact under a condition of 3.5 mg/L for 20 min, 1.5 mL of the activated sludge mixed solution was transferred by a pipette and added into a mL of centrifuge tube; after taken out of a refrigerator, a dye | and a dye II were placed at a room temperature for 5 min so as to unfreeze; then 10 JL of the dye | and 10 uL of the dye II were 5 mixed by a microsyringe under a shade condition and added into a 10 mL of centrifuge tube; 6.0 mL of deionized water was added to dilute (having a volume ratio of 1:300), and the solution was taken as a standard stained mixed solution so as to preserve in shade; 15 JL of the above standard stained mixed solution was added into 1.5 mL of the sampled activated sludge mixed solution (having a volume ratio of 1:100) by the microsyringe, standing was performed after shaking up, and staining was performed in shade for 15 min; then, a certain amount of fluorescently stained activated sludge was absorbed by a dropper and dropped onto a glass slide cleaned with ultrapure water, and cover glass slightly covered on the glass slide; the activated sludge was enabled to image clearly by adjusting a coarse/fine adjustment spiral of a microscope; a lens of the microscope was adjusted to a corresponding fluorescent channel; a CCD camera client on a computer was started, and imaging of the microscope was enabled to be displayed on a computer screen in real time; and a rotary knob was manually adjusted to move the observed sample, wherein 40 photos were acquired in each group.
The fluorescence intensity and fluorescent area of the fluorescent activated sludge in each photo were analyzed, and obtained results of green fluorescence intensity OD and an inhibition ratio | of the mass transfer performance per unit area were as shown in Table 1.
Table 1 Data table of OD and | under different cyclohexane concentrations
0.10642+/- | 0.07683+/- 0.0672+/- 0.05041+/- 0.0294+/- [ome | oo | ome | om | om Te || en | weer | wees | mem Through calculation, the mean relative standard deviation of OD of 5 groups of samples is respectively 0.705%, 0.854%, 0.987%, 1.131% and 0.615% and is lower than 5%, which indicates that data reproducibility is good. In addition, the mean red fluorescent areas of the 5 groups of samples are respectively 2.0%, 2.3%, 2.9%, 3.1% and 3.8% and are all lower than 5%, which indicates that activity of the activated sludge is excellent. Therefore, the fluorescence intensity may serve as a characterization result of the mass transfer performance. It can be seen from results of the Table 1 that, with an increase of the cyclohexane concentrations, the fluorescence intensity is gradually decreased, the mass transfer inhibition ratio is gradually increased, the mass transfer performance is decreased, and a mass transfer performance inhibition effect is increased. By detecting whether the water quality in the system is up to the standard, an appropriate ratio of the toxic substances to the activated sludge in the system can be conveniently deduced.
Embodiment 2 A test sample was actual refinery wastewater containing characteristic toxic organic matters. A nutrition matrix was added into the actual wastewater according to a volume ratio of 1:2; an addition amount of the test sample added with the nutrition matrix was 600 mL; the test sample was mixed with 300 mL of activated sludge (having a volume ratio of 1:2), and a total reaction system was 900 mL; a concentration of mixed liquor suspended solids (MLSS) in the system was about 1.2 g/L, and the solution was uniformly stirred by a glass rod; after dissolved oxygen was subjected to aerobic aeration and full contact under a condition of 2.5 mg/L for 20 min, 1.5 mL of the activated sludge mixed solution was transferred by a pipette and added into a 5 mL of centrifuge tube; after taken out of a refrigerator, a dye | and a dye II were placed at a room temperature for 5 min so as to unfreeze; then 10 uL of the dye | and 10 LL of the dye II were mixed by a microsyringe under a shade condition and added into a 10 mL of centrifuge tube; 60 mL of deionized water was added to dilute (having a volume ratio of 1:300), and the solution was taken as a standard stained mixed solution so as to preserve in shade; 15 ‚L of the above standard stained mixed solution was added into 15 mL of the sampled activated sludge mixed solution (having a volume ratio of 1:1000) by the microsyringe, standing was performed after shaking up, and staining was performed in shade for 15 min; then, a certain amount of fluorescently stained activated sludge was absorbed by a dropper and dropped onto a glass slide cleaned with ultrapure water, and cover glass slightly covered on the glass slide; the activated sludge was enabled to image clearly by adjusting a coarse/fine adjustment spiral of a microscope; a lens of the microscope was adjusted to a corresponding fluorescent channel; a CCD camera client on a computer was started, and imaging of the microscope was enabled to be displayed on a computer screen in real time; and a rotary knob was manually adjusted to move the observed sample, wherein 40 photos were acquired in each group.
Through calculation, the value OD in the blank group is 0.10582+0.006, and the value OD in the test group and the inhibition ratio | of the mass transfer performance are respectively
0.0971310.02 and 20.1014.4%.
The relative standard deviation of the OD in the test group of the refinery wastewater is 1.75% and is lower than 5%, which indicates that the data reproducibility is good. The mean red fluorescent areas in the blank group and the test group are respectively 1.7% and 2.4% and are all lower than 5%, which indicates that the activity of the activated sludge is excellent. Therefore, the fluorescence intensity may serve as a characterization result of the mass transfer performance.
Embodiment 3
A test sample was actual ABS resin wastewater. A nutrition matrix was added into the actual wastewater according to a volume ratio of 1:2; an addition amount of the test wastewater sample added with the nutrition matrix was 600 mL; the test sample was mixed with 300 mL of activated sludge, and a total reaction system was 900 mL; a concentration of mixed liquor suspended solids (MLSS) in the system was about 1.2 g/L, and the solution was uniformly stirred by a glass rod; after dissolved oxygen was subjected to aerobic aeration and full contact under a condition of 4.0 mg/L for 20 min, 1.5 mL of the activated sludge mixed solution was transferred by a pipette and added into a 5 mL of centrifuge tube; after taken out of a refrigerator, a dye | and a dye Il were placed at a room temperature for 5 min so as to unfreeze; then 10 yL of the dye | and 10 pL of the dye II were mixed by a microsyringe under a shade condition and added into a 10 mL of centrifuge tube; 6.0 mL of deionized water was added to dilute, and the solution was taken as a standard stained mixed solution so as to preserve in shade; 15 jL of the above standard stained mixed solution was added into 1.5 mL of the sampled activated sludge mixed solution by the microsyringe, standing was performed after shaking up, and staining was performed in shade for 15 min; then, a certain amount of fluorescently stained activated sludge was absorbed by a dropper and dropped onto a glass slide cleaned with ultrapure water, and cover glass slightly covered on the glass slide; the activated sludge was enabled to image clearly by adjusting a coarse/fine adjustment spiral of a microscope; a lens of the microscope was adjusted to a corresponding fluorescent channel; a CCD camera client on a computer was started, and imaging of the microscope was enabled to be displayed on a computer screen in real time; and a rotary knob was manually adjusted to move the observed sample, wherein 40 photos were acquired in each group.
Through calculation, the value OD in the blank group is 0.10782+0.004, and the value OD in the test group and the inhibition ratio | of the mass transfer performance are respectively
0.0791210.02 and 29.08+2.5%.
The relative standard deviation of the OD in the test group of the ABS resin wastewater is
2.79% and is lower than 5%, which indicates that the data reproducibility is good. The mean red fluorescent areas in the blank group and the test group are respectively 1.4% and 2.8% and are all lower than 5%, which indicates that the activity of the activated sludge is excellent. Therefore, the fluorescence intensity may serve as a characterization result of the mass transfer performance.
The above embodiments only express multiple embodiments of the present invention. Descriptions of the embodiments are relatively specific and detailed, but shall not be understood as a limitation to the scope of the patent of the present invention. It should be indicated that, several modifications and improvements may be made by those ordinary skilled in the art without departing from the concept of the present invention. The modifications and improvements belong to the protection scope of the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2026316A NL2026316B1 (en) | 2020-08-20 | 2020-08-20 | Method for evaluating influences of toxic substances on mass transfer performance of activated sludge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2026316A NL2026316B1 (en) | 2020-08-20 | 2020-08-20 | Method for evaluating influences of toxic substances on mass transfer performance of activated sludge |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL2026316B1 true NL2026316B1 (en) | 2021-03-15 |
Family
ID=75222350
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL2026316A NL2026316B1 (en) | 2020-08-20 | 2020-08-20 | Method for evaluating influences of toxic substances on mass transfer performance of activated sludge |
Country Status (1)
| Country | Link |
|---|---|
| NL (1) | NL2026316B1 (en) |
-
2020
- 2020-08-20 NL NL2026316A patent/NL2026316B1/en not_active IP Right Cessation
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1044070B1 (en) | Device for determination of an analyte in a solution | |
| DE102011088959B4 (en) | Device for degassing a liquid and use of this device in an analysis device | |
| DE60315043T2 (en) | IDENTIFICATION OF BIOLOGICAL MOLECULES BY DIFFERENTIAL DISTRIBUTION OF ENZYME SUBSTRATES AND PRODUCTS | |
| CN105548106A (en) | Method for in-situ synchronous acquisition of two-dimensional distribution of active phosphor and dissolved oxygen in water, soil or sediment | |
| US20130130308A1 (en) | Process for directly measuring multiple biodegradabilities | |
| CN105277535B (en) | Ammonia nitrogen field fast detection method in a kind of water for eliminating reagent blank influence | |
| CN109959643B (en) | Method for evaluating influence of toxic substances on mass transfer performance of activated sludge | |
| CN109852664B (en) | Method for detecting single algae biomass in water body of water bloom or red tide | |
| CN102411001A (en) | Method for rapid detection of hexavalent chromium ions in sewage | |
| NL2026316B1 (en) | Method for evaluating influences of toxic substances on mass transfer performance of activated sludge | |
| CN107764757B (en) | Device, system, preparation method and target object content determination method for detecting target ion content and kit | |
| EP1238096B1 (en) | Method and apparatus for prokaryotic and eukaryotic cell quantitation | |
| CN106645046B (en) | Method for in-situ determination of intracellular nitric oxide of denitrification type activated sludge microorganisms | |
| WO2006042439A1 (en) | Automatable method for determining assimilable organic carbon and assessing microbial growth inhibition in water | |
| CN111024689B (en) | White spirit alcoholic strength detection method based on color-changing nano material | |
| CN1243232C (en) | Photochemical and biochemical microorganic membrane dynamic responding sensor for COD with optical fiber | |
| CN108375610B (en) | Correction method for analyzing sludge enzyme activity based on oxygen consumption rate inhibition rate | |
| CN209231353U (en) | A kind of water quality automatic monitoring device | |
| CN110790376A (en) | Method for evaluating biological treatment characteristics of wastewater | |
| CN108168984A (en) | A kind of protein PAGE gel electrophoresis rapid dyeing kits and colouring method | |
| RU2306557C1 (en) | Method of estimating quality of antocyan dye prepared via anoxic way | |
| CN118209546B (en) | Rapid compost maturity detection method | |
| CN219861387U (en) | Nucleic acid quantitative detection instrument based on color change of hydroxynaphthol blue indicator | |
| CN112557386B (en) | Identification method of microorganism capable of catalyzing alkane molecules in petroleum to produce long-chain fatty acid | |
| CN116380854A (en) | Method for rapidly detecting microplastic pollution in water based on fluorescence spectrum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM | Lapsed because of non-payment of the annual fee |
Effective date: 20230901 |