MY191182A - Method for extracting roselle extractive and therapeutic application thereof - Google Patents

Abstract

Disclosed is a method of obtaining a Roselle calyces extract with an increased quantity of anthocyanins. The method includes a step of continuously feeding a solvent into an extraction column having an exit end at a pre-defined pressure, a pre-defined temperature and a pre-defined solvent flow rate to ensure saturation at the exit end of the extraction column. The solvent comprises carbon dioxide and a modifier. Then the method includes a step of utilizing supercritical carbon dioxide-ethanol (ScCO2) with the modifier to enhance polarity of the solvent extracted from the extraction column. The modifier is a mixture of ethanol and water at 3:1 (v/v). Further, the method includes a step of pumping the solvent and the modifier at a flow rate of 6 milliliters per minute (mL/min) to obtain the Roselle calyces extract in a pre-defined time duration. The solvent having anthocyanins molecules in an equilibrium between a colored cationic form and a colorless pseudo base. The equilibrium is influenced by a pH condition. The pH condition determines the color of the anthocyanins molecules. The anthocyanins molecules having a red circle structure. The red circle structure is flavylium cation. The anthocyanins molecules form at least one of a negative ion, and anion at a higher pH condition. Figure 1