MY173904A - Application of enzyme-quantum dots hybrid system for the determination of uric acid - Google Patents

Application of enzyme-quantum dots hybrid system for the determination of uric acid

Info

Publication number
MY173904A
MY173904A MYPI2013003147A MYPI2013003147A MY173904A MY 173904 A MY173904 A MY 173904A MY PI2013003147 A MYPI2013003147 A MY PI2013003147A MY PI2013003147 A MYPI2013003147 A MY PI2013003147A MY 173904 A MY173904 A MY 173904A
Authority
MY
Malaysia
Prior art keywords
uric acid
hybrid system
determination
uricase
hrp
Prior art date
Application number
MYPI2013003147A
Inventor
Ellina Azmi Nur
Izaanin Ramli Noor
Abdullah Jaafar
Sidek Hamidah
Abd Rahman Samsulida
Ariffin Nurhayati
Azmi Abdul Hamid Muhammad
Original Assignee
Sirim Berhad
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sirim Berhad filed Critical Sirim Berhad
Priority to MYPI2013003147A priority Critical patent/MY173904A/en
Priority to PCT/MY2014/000256 priority patent/WO2015047079A2/en
Publication of MY173904A publication Critical patent/MY173904A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C12N9/0046—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7) with oxygen as acceptor (1.7.3)
    • C12N9/0048—Uricase (1.7.3.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/62—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving uric acid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Nanotechnology (AREA)
  • Materials Engineering (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The present invention relates to a uricase/HRP-CdS QDs hydrid system for the determination of uric acid. The QDs was used as an electron donor, whereas uricase and HRP (horseradish peroxidase) were used as acceptgrs for the oxidation/reduction reactions involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence which was proportional to uric acid concentration. The hybrid system demonstrated maximum activity of uricase/HRP at ratio of 111 and pH 7.0, respectively. The linearity of the hybrid system towards uric acid was in the concentration range of 125-1000 ?M with detection limit of 125 ?M.
MYPI2013003147A 2013-08-27 2013-08-27 Application of enzyme-quantum dots hybrid system for the determination of uric acid MY173904A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MYPI2013003147A MY173904A (en) 2013-08-27 2013-08-27 Application of enzyme-quantum dots hybrid system for the determination of uric acid
PCT/MY2014/000256 WO2015047079A2 (en) 2013-08-27 2014-10-23 Application of enzyme-quantum dots hybrid system for the determination of uric acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2013003147A MY173904A (en) 2013-08-27 2013-08-27 Application of enzyme-quantum dots hybrid system for the determination of uric acid

Publications (1)

Publication Number Publication Date
MY173904A true MY173904A (en) 2020-02-26

Family

ID=52463067

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2013003147A MY173904A (en) 2013-08-27 2013-08-27 Application of enzyme-quantum dots hybrid system for the determination of uric acid

Country Status (2)

Country Link
MY (1) MY173904A (en)
WO (1) WO2015047079A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105784660B (en) * 2016-04-05 2018-10-19 广西师范学院 Utilize the method for water-soluble InP/ZnS QDs probe in detecting horseradish peroxidase concentration
CN106872422B (en) * 2016-12-30 2018-04-03 锦州医科大学 The method of uric acid in quantum dots characterization body fluid
CN110726707B (en) * 2019-10-30 2022-08-23 南京医科大学 Based on N-Ti 3 C 2 Composite nano probe of QDs and o-phenylenediamine oxide and detection method thereof
DK180929B1 (en) 2021-02-25 2022-06-29 Univ Aarhus System and method for balancing a vanadium redox flow battery
CN115612486B (en) * 2022-10-31 2024-02-13 扬州大学 Cobalt/manganese dioxide quantum dot with uricase-like activity and preparation method thereof

Also Published As

Publication number Publication date
WO2015047079A3 (en) 2015-07-02
WO2015047079A2 (en) 2015-04-02

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