MY172900A - An electrochemical dna biosensor for gender and variety identification - Google Patents

An electrochemical dna biosensor for gender and variety identification

Info

Publication number
MY172900A
MY172900A MYPI2015701671A MYPI2015701671A MY172900A MY 172900 A MY172900 A MY 172900A MY PI2015701671 A MYPI2015701671 A MY PI2015701671A MY PI2015701671 A MYPI2015701671 A MY PI2015701671A MY 172900 A MY172900 A MY 172900A
Authority
MY
Malaysia
Prior art keywords
dna biosensor
electrochemical dna
oligonucleotide sequence
fish
gender
Prior art date
Application number
MYPI2015701671A
Inventor
Yook Heng Lee
Abd Rashid Zulkafli
Poh Chiang Chew
Futra Dedi
Original Assignee
Univ Kebangsaan Malaysia
Jabatan Perikanan Malaysia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kebangsaan Malaysia, Jabatan Perikanan Malaysia filed Critical Univ Kebangsaan Malaysia
Priority to MYPI2015701671A priority Critical patent/MY172900A/en
Priority to CN201680043018.7A priority patent/CN107922978A/en
Priority to PCT/MY2016/000031 priority patent/WO2016190727A1/en
Publication of MY172900A publication Critical patent/MY172900A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3276—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a hybridisation with immobilised receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Nanotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

An electrochemical DNA biosensor comprising a screen-printed electrode modified with gold nanoparticles, a layer of silica nanosphere composite, an oligonucleotide sequence, a cross-linking agent and a dry reagent pad having a buffer salt and a redox indicator. The layer of silica nanosphere composite is deposited on the screen-printed electrode and the oligonucleotide sequence is immobilized on the layer of silica nanosphere composite using the cross-linking agent. Also, at least a portion of the oligonucleotide sequence is complementary to a unique target sequence of a nucleic acid from a fish of interest such that the nucleic acid from the fish of interest hybridizes to the oligonucleotide sequence to form a dsDNA. In addition, the redox indicator intercalates with the dsDNA which enables detection of gender and variety of the fish by voltammetry. Further, the electrochemical DNA biosensor is a reagentless electrochemical DNA biosensor.
MYPI2015701671A 2015-05-22 2015-05-22 An electrochemical dna biosensor for gender and variety identification MY172900A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
MYPI2015701671A MY172900A (en) 2015-05-22 2015-05-22 An electrochemical dna biosensor for gender and variety identification
CN201680043018.7A CN107922978A (en) 2015-05-22 2016-05-23 The electrochemical DNA biosensor identified for gender and classification
PCT/MY2016/000031 WO2016190727A1 (en) 2015-05-22 2016-05-23 An electrochemical dna biosensor for gender and variety identification

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2015701671A MY172900A (en) 2015-05-22 2015-05-22 An electrochemical dna biosensor for gender and variety identification

Publications (1)

Publication Number Publication Date
MY172900A true MY172900A (en) 2019-12-13

Family

ID=57393381

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2015701671A MY172900A (en) 2015-05-22 2015-05-22 An electrochemical dna biosensor for gender and variety identification

Country Status (3)

Country Link
CN (1) CN107922978A (en)
MY (1) MY172900A (en)
WO (1) WO2016190727A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11946098B2 (en) 2017-06-01 2024-04-02 The Regents Of The University Of California Calibration-free measurement with electrochemical biosensors
CN113711040A (en) * 2019-02-27 2021-11-26 加州理工学院 Electrochemical sensing method for molecular quantification in body fluids

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005241112B2 (en) * 2001-07-13 2009-07-30 Nanosphere, Inc. Method for preparing substrates having immobilized molecules and substrates
WO2010117341A1 (en) * 2009-04-08 2010-10-14 Agency For Science, Technology And Research Nucleic acid biosensor
US20130334063A1 (en) * 2012-06-15 2013-12-19 Gordon & Rosenblatt, Llc Method of Detecting Analyte
CN103760201A (en) * 2013-12-10 2014-04-30 天津工业大学 Preparation method of composite quantum dot-based electrochemical DNA sensor

Also Published As

Publication number Publication date
WO2016190727A1 (en) 2016-12-01
CN107922978A (en) 2018-04-17

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