MY165920A - A protein extraction kit from plant material - Google Patents

A protein extraction kit from plant material

Info

Publication number
MY165920A
MY165920A MYPI2011700031A MYPI2011700031A MY165920A MY 165920 A MY165920 A MY 165920A MY PI2011700031 A MYPI2011700031 A MY PI2011700031A MY PI2011700031 A MYPI2011700031 A MY PI2011700031A MY 165920 A MY165920 A MY 165920A
Authority
MY
Malaysia
Prior art keywords
protein
inventive method
plant material
extraction kit
protein extraction
Prior art date
Application number
MYPI2011700031A
Inventor
Rastegari Elham
Farringdon Spencer David
Ahmad Zalinah
Ismail Maznah
Original Assignee
Univ Putra Malaysia Upm
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Putra Malaysia Upm filed Critical Univ Putra Malaysia Upm
Priority to MYPI2011700031A priority Critical patent/MY165920A/en
Priority to PCT/MY2012/000044 priority patent/WO2012134262A2/en
Publication of MY165920A publication Critical patent/MY165920A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C07K1/128—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/0201—Pectin lyase (4.2.2.10)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

The present invention relates to methods for extracting protein from plant material which is characterized by a high content of interfering compounds, specifically abundant polysaccharides like pectin. The inventive method applies a pectolytic treatment in preparation of the plant material sample prior to protein extraction. This optimized method results in high quality protein extracts compatible with further sensitive proteomic analyses specifically methods like two dimensional gel electrophoresis or mass spectrometry. The present invention further relates to the protein extracts obtained by the inventive method and the use of the inventive method in the preparation of protein samples. Also a protein extraction kit is disclosed for conducting the inventive method, as well as the use of the protein extraction kit in protein analysis and proteomics.
MYPI2011700031A 2011-03-30 2011-03-30 A protein extraction kit from plant material MY165920A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MYPI2011700031A MY165920A (en) 2011-03-30 2011-03-30 A protein extraction kit from plant material
PCT/MY2012/000044 WO2012134262A2 (en) 2011-03-30 2012-03-07 A method for extracting protein from pectin rich plant material

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2011700031A MY165920A (en) 2011-03-30 2011-03-30 A protein extraction kit from plant material

Publications (1)

Publication Number Publication Date
MY165920A true MY165920A (en) 2018-05-18

Family

ID=46932197

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2011700031A MY165920A (en) 2011-03-30 2011-03-30 A protein extraction kit from plant material

Country Status (2)

Country Link
MY (1) MY165920A (en)
WO (1) WO2012134262A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104020296A (en) * 2014-06-09 2014-09-03 无锡福阳生物科技有限公司 Quantitative detection method of transgenosis protein CP4EPSPS
EP3959223A4 (en) * 2019-04-25 2023-07-19 Flagship Pioneering Innovations VI, LLC Compositions and methods relating to plant messenger packs
CN111592586B (en) * 2019-11-24 2021-12-03 杭州市农业科学研究院 Strawberry pistil protein extraction method suitable for mass spectrum identification
CN111171108B (en) * 2019-12-27 2021-12-10 中国热带农业科学院南亚热带作物研究所 Efficient extraction method of total proteins of macadamia leaf
CN112641101A (en) * 2020-12-24 2021-04-13 常州市芙丽佳生物科技有限公司 Albumen powder containing olive kernel extract and preparation method thereof
CN112617198A (en) * 2020-12-24 2021-04-09 常州市芙丽佳生物科技有限公司 Preparation method of olive kernel extract

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830738A (en) * 1996-06-04 1998-11-03 Clemson University Extraction of pigment from plant material
US6313273B1 (en) * 1999-08-25 2001-11-06 Abbott Laboratories Soy proteins and methods for their production

Also Published As

Publication number Publication date
WO2012134262A2 (en) 2012-10-04
WO2012134262A3 (en) 2012-12-27

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