MY165920A - A protein extraction kit from plant material - Google Patents
A protein extraction kit from plant materialInfo
- Publication number
- MY165920A MY165920A MYPI2011700031A MYPI2011700031A MY165920A MY 165920 A MY165920 A MY 165920A MY PI2011700031 A MYPI2011700031 A MY PI2011700031A MY PI2011700031 A MYPI2011700031 A MY PI2011700031A MY 165920 A MY165920 A MY 165920A
- Authority
- MY
- Malaysia
- Prior art keywords
- protein
- inventive method
- plant material
- extraction kit
- protein extraction
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
- C07K1/128—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general sequencing
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01015—Polygalacturonase (3.2.1.15)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
- C12Y402/0201—Pectin lyase (4.2.2.10)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Computational Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The present invention relates to methods for extracting protein from plant material which is characterized by a high content of interfering compounds, specifically abundant polysaccharides like pectin. The inventive method applies a pectolytic treatment in preparation of the plant material sample prior to protein extraction. This optimized method results in high quality protein extracts compatible with further sensitive proteomic analyses specifically methods like two dimensional gel electrophoresis or mass spectrometry. The present invention further relates to the protein extracts obtained by the inventive method and the use of the inventive method in the preparation of protein samples. Also a protein extraction kit is disclosed for conducting the inventive method, as well as the use of the protein extraction kit in protein analysis and proteomics.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MYPI2011700031A MY165920A (en) | 2011-03-30 | 2011-03-30 | A protein extraction kit from plant material |
PCT/MY2012/000044 WO2012134262A2 (en) | 2011-03-30 | 2012-03-07 | A method for extracting protein from pectin rich plant material |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MYPI2011700031A MY165920A (en) | 2011-03-30 | 2011-03-30 | A protein extraction kit from plant material |
Publications (1)
Publication Number | Publication Date |
---|---|
MY165920A true MY165920A (en) | 2018-05-18 |
Family
ID=46932197
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MYPI2011700031A MY165920A (en) | 2011-03-30 | 2011-03-30 | A protein extraction kit from plant material |
Country Status (2)
Country | Link |
---|---|
MY (1) | MY165920A (en) |
WO (1) | WO2012134262A2 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104020296A (en) * | 2014-06-09 | 2014-09-03 | 无锡福阳生物科技有限公司 | Quantitative detection method of transgenosis protein CP4EPSPS |
EP3959223A4 (en) * | 2019-04-25 | 2023-07-19 | Flagship Pioneering Innovations VI, LLC | Compositions and methods relating to plant messenger packs |
CN111592586B (en) * | 2019-11-24 | 2021-12-03 | 杭州市农业科学研究院 | Strawberry pistil protein extraction method suitable for mass spectrum identification |
CN111171108B (en) * | 2019-12-27 | 2021-12-10 | 中国热带农业科学院南亚热带作物研究所 | Efficient extraction method of total proteins of macadamia leaf |
CN112641101A (en) * | 2020-12-24 | 2021-04-13 | 常州市芙丽佳生物科技有限公司 | Albumen powder containing olive kernel extract and preparation method thereof |
CN112617198A (en) * | 2020-12-24 | 2021-04-09 | 常州市芙丽佳生物科技有限公司 | Preparation method of olive kernel extract |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5830738A (en) * | 1996-06-04 | 1998-11-03 | Clemson University | Extraction of pigment from plant material |
US6313273B1 (en) * | 1999-08-25 | 2001-11-06 | Abbott Laboratories | Soy proteins and methods for their production |
-
2011
- 2011-03-30 MY MYPI2011700031A patent/MY165920A/en unknown
-
2012
- 2012-03-07 WO PCT/MY2012/000044 patent/WO2012134262A2/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2012134262A2 (en) | 2012-10-04 |
WO2012134262A3 (en) | 2012-12-27 |
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