MY163800A - A method of cultivating hendersonia spp. - Google Patents

A method of cultivating hendersonia spp.

Info

Publication number
MY163800A
MY163800A MYUI2014702074A MYUI2014702074A MY163800A MY 163800 A MY163800 A MY 163800A MY UI2014702074 A MYUI2014702074 A MY UI2014702074A MY UI2014702074 A MYUI2014702074 A MY UI2014702074A MY 163800 A MY163800 A MY 163800A
Authority
MY
Malaysia
Prior art keywords
culture
produce
hendersonia
spp
stirred tank
Prior art date
Application number
MYUI2014702074A
Inventor
Then @ Then Khoon Thiam Charles
Danial Bin Azman Muhammad
Wei Chang H'ng
Abu Seman IDRIS
Ali Metwally Ali El-Enshasy Hesham
Original Assignee
All Cosmos Ind Sdn Bhd
Malaysia Palm Oil Board
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by All Cosmos Ind Sdn Bhd, Malaysia Palm Oil Board filed Critical All Cosmos Ind Sdn Bhd
Priority to MYUI2014702074A priority Critical patent/MY163800A/en
Publication of MY163800A publication Critical patent/MY163800A/en

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Abstract

The present invention relates to a method for producing Hendersonia spp. cultures, characterized by the steps of culturing Hendersonia spp. cultures on activation agar until maturity; inoculating sterile distilled water with the cultured Hendersonia spp. to produce a cell suspension; inoculating an activation medium with the cell suspension; incubating the inoculated activation medium in a shaking incubator to produce a vegetative culture of Hendersonia spp.; transferring the vegetative culture to a first stirred tank bioreactor containing a first production medium, followed by incubation to produce a first seed culture; transferring the first seed culture to a second stirred tank bioreactor containing a second production medium, followed by incubation to produce a second seed culture; transferring the second seed culture to a third stirred tank bioreactor containing a fresh batch of the second production medium, followed by incubation to produce a mature Hendersonia spp. culture; and homogenizing the mature Hendersonia spp. culture; wherein dissolved oxygen tension is maintained at a minimum of 30% in each stirred tank bioreactor; wherein the first and second seed cultures each achieves a minimum cell dry weight of 14 g/L before transfer to the second and third bioreactors respectively.
MYUI2014702074A 2014-07-25 2014-07-25 A method of cultivating hendersonia spp. MY163800A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MYUI2014702074A MY163800A (en) 2014-07-25 2014-07-25 A method of cultivating hendersonia spp.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYUI2014702074A MY163800A (en) 2014-07-25 2014-07-25 A method of cultivating hendersonia spp.

Publications (1)

Publication Number Publication Date
MY163800A true MY163800A (en) 2017-10-24

Family

ID=60324526

Family Applications (1)

Application Number Title Priority Date Filing Date
MYUI2014702074A MY163800A (en) 2014-07-25 2014-07-25 A method of cultivating hendersonia spp.

Country Status (1)

Country Link
MY (1) MY163800A (en)

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