MY151978A - One step multiplex system for the detection of salmonella spp., s. typhi and s. paratyphi a with an internal control - Google Patents

One step multiplex system for the detection of salmonella spp., s. typhi and s. paratyphi a with an internal control

Info

Publication number
MY151978A
MY151978A MYPI2011005414A MY151978A MY 151978 A MY151978 A MY 151978A MY PI2011005414 A MYPI2011005414 A MY PI2011005414A MY 151978 A MY151978 A MY 151978A
Authority
MY
Malaysia
Prior art keywords
paratyphi
typhi
salmonella spp
present
internal control
Prior art date
Application number
Inventor
Thong Kwai Prof Dr Lin
Madya Dr Chua Kek Prof Heng
Original Assignee
Univ Malaya
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Malaya filed Critical Univ Malaya
Priority to MYPI2011005414 priority Critical patent/MY151978A/en
Priority to PCT/MY2012/000271 priority patent/WO2013070060A1/en
Priority to PH12014501312A priority patent/PH12014501312A1/en
Publication of MY151978A publication Critical patent/MY151978A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

THE PRESENT INVENTION DISCLOSES A SYSTEM WHICH CONSISTS OF 4 PAIRS OF PRIMERS THAT SPECIFICALLY DETECT SALMONELLA SPP., S. TYPHI AND S. PARATYPHI A WITH AN INTERNAL CONTROL. THE SYSTEM, WHEN APPLIED IN POLYMERASE CHAIN REACTION (PCR) UNDER SPECIFIC CONDITIONS, REACTION MIXTURE AND CYCLING TEMPERATURES PRODUCE FOUR BANDS 784BP, 496BP, 332BP AND 187BP. THE DNA BAND 784BP IS PRESENT IN ALL SALMONELLA SPP., WHILE THE BANDS OF 496BP AND 332BP ARE ONLY PRESENT IN S. PARATYPHI A AND S. TYPHI RESPECTIVELY. AN INTERNAL AMPLIFICATION CONTROL AS INDICATED BY THE 187BP SHOULD BE PRESENT IN ALL THE TESTS.
MYPI2011005414 2011-11-09 2011-11-09 One step multiplex system for the detection of salmonella spp., s. typhi and s. paratyphi a with an internal control MY151978A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
MYPI2011005414 MY151978A (en) 2011-11-09 2011-11-09 One step multiplex system for the detection of salmonella spp., s. typhi and s. paratyphi a with an internal control
PCT/MY2012/000271 WO2013070060A1 (en) 2011-11-09 2012-11-09 One step multiplex system for the detection of salmonella spp., s. typhi and s. paratyphi a with an internal control
PH12014501312A PH12014501312A1 (en) 2011-11-09 2014-06-09 One step multiplex system for the detection of salmonella spp., s. typhi and s. paratyphi a with an internal control

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2011005414 MY151978A (en) 2011-11-09 2011-11-09 One step multiplex system for the detection of salmonella spp., s. typhi and s. paratyphi a with an internal control

Publications (1)

Publication Number Publication Date
MY151978A true MY151978A (en) 2014-07-31

Family

ID=48290349

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2011005414 MY151978A (en) 2011-11-09 2011-11-09 One step multiplex system for the detection of salmonella spp., s. typhi and s. paratyphi a with an internal control

Country Status (3)

Country Link
MY (1) MY151978A (en)
PH (1) PH12014501312A1 (en)
WO (1) WO2013070060A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112961805B (en) * 2020-12-17 2022-05-20 广东省科学院微生物研究所(广东省微生物分析检测中心) Salmonella typhimurium with quinolone drug resistance genes gyrA and parE mutated simultaneously and application thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004087958A1 (en) * 2003-03-31 2004-10-14 Council Of Scientific And Industrial Research Stn gene oligonucleotide primers for detecting salmonella species and detection prosses using the same
EP1895014A1 (en) * 2006-09-01 2008-03-05 Bio-Rad Pasteur Detection of salmonella by real-time multiplex PCR
WO2011001449A2 (en) * 2009-06-18 2011-01-06 Indian Institute Of Science Primers, pcr reaction mixture and methods thereof

Also Published As

Publication number Publication date
PH12014501312A1 (en) 2014-10-08
WO2013070060A1 (en) 2013-05-16

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