MXPA01008626A - Inhibidor novedoso de muerte programada de celula. - Google Patents

Info

Publication number

MXPA01008626A

MXPA01008626A MXPA01008626A MXPA01008626A MXPA01008626A MX PA01008626 A MXPA01008626 A MX PA01008626A MX PA01008626 A MXPA01008626 A MX PA01008626A MX PA01008626 A MXPA01008626 A MX PA01008626A MX PA01008626 A MXPA01008626 A MX PA01008626A

Authority

MX

Mexico

Prior art keywords

aven

amino acid

sequence

vector

dna

Prior art date

1999-02-26

Links

• Espacenet
• Global Dossier
• Discuss

• 239000003112 inhibitor Substances 0.000 title description 17
• 238000003782 apoptosis assay Methods 0.000 title description 5
• 230000005522 programmed cell death Effects 0.000 title description 5
• 230000014509 gene expression Effects 0.000 claims abstract description 74
• 239000012634 fragment Substances 0.000 claims abstract description 69
• 230000006907 apoptotic process Effects 0.000 claims abstract description 65
• 150000007523 nucleic acids Chemical group 0.000 claims abstract description 55
• 102000040430 polynucleotide Human genes 0.000 claims abstract description 40
• 108091033319 polynucleotide Proteins 0.000 claims abstract description 40
• 239000002157 polynucleotide Substances 0.000 claims abstract description 40
• -1 antibodies Substances 0.000 claims abstract description 36
• 230000000692 anti-sense effect Effects 0.000 claims abstract description 27
• 230000027455 binding Effects 0.000 claims abstract description 25
• 230000000295 complement effect Effects 0.000 claims abstract description 22
• 238000004519 manufacturing process Methods 0.000 claims abstract description 22
• 239000005557 antagonist Substances 0.000 claims abstract description 14
• 108090000623 proteins and genes Proteins 0.000 claims description 142
• 238000000034 method Methods 0.000 claims description 106
• 102000004169 proteins and genes Human genes 0.000 claims description 96
• 108091028043 Nucleic acid sequence Proteins 0.000 claims description 70
• 239000013598 vector Substances 0.000 claims description 68
• 150000001413 amino acids Chemical class 0.000 claims description 61
• 108020004414 DNA Proteins 0.000 claims description 54
• 125000003729 nucleotide group Chemical group 0.000 claims description 53
• 239000002773 nucleotide Substances 0.000 claims description 51
• 125000003275 alpha amino acid group Chemical group 0.000 claims description 42
• 238000009396 hybridization Methods 0.000 claims description 39
• 102100026596 Bcl-2-like protein 1 Human genes 0.000 claims description 35
• 239000000523 sample Substances 0.000 claims description 35
• 102000039446 nucleic acids Human genes 0.000 claims description 33
• 108020004707 nucleic acids Proteins 0.000 claims description 33
• 239000000203 mixture Substances 0.000 claims description 27
• 241001465754 Metazoa Species 0.000 claims description 18
• 150000001875 compounds Chemical class 0.000 claims description 18
• 102000010565 Apoptosis Regulatory Proteins Human genes 0.000 claims description 16
• 108010063104 Apoptosis Regulatory Proteins Proteins 0.000 claims description 16
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims description 12
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims description 12
• 230000035899 viability Effects 0.000 claims description 12
• 230000009223 neuronal apoptosis Effects 0.000 claims description 9
• 230000004071 biological effect Effects 0.000 claims description 8
• 238000002560 therapeutic procedure Methods 0.000 claims description 8
• 238000004113 cell culture Methods 0.000 claims description 7
• 239000003937 drug carrier Substances 0.000 claims description 7
• 238000002347 injection Methods 0.000 claims description 7
• 239000007924 injection Substances 0.000 claims description 7
• 238000001890 transfection Methods 0.000 claims description 7
• 230000001965 increasing effect Effects 0.000 claims description 6
• 230000001737 promoting effect Effects 0.000 claims description 6
• 230000002401 inhibitory effect Effects 0.000 claims description 5
• 101001056180 Homo sapiens Induced myeloid leukemia cell differentiation protein Mcl-1 Proteins 0.000 claims description 4
• 102100026539 Induced myeloid leukemia cell differentiation protein Mcl-1 Human genes 0.000 claims description 4
• 230000001976 improved effect Effects 0.000 claims description 4
• 239000002502 liposome Substances 0.000 claims description 4
• 230000025915 regulation of apoptotic process Effects 0.000 claims description 4
• 101150039936 ced-9 gene Proteins 0.000 claims description 3
• 239000012472 biological sample Substances 0.000 claims description 2
• 108020004711 Nucleic Acid Probes Proteins 0.000 claims 1
• 239000002853 nucleic acid probe Substances 0.000 claims 1
• 101100004031 Mus musculus Aven gene Proteins 0.000 abstract description 363
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 38
• 201000010099 disease Diseases 0.000 abstract description 31
• 238000003556 assay Methods 0.000 abstract description 20
• 238000011282 treatment Methods 0.000 abstract description 16
• 239000013604 expression vector Substances 0.000 abstract description 14
• 239000000556 agonist Substances 0.000 abstract description 9
• 230000002265 prevention Effects 0.000 abstract description 5
• 102000034356 gene-regulatory proteins Human genes 0.000 abstract 1
• 108091006104 gene-regulatory proteins Proteins 0.000 abstract 1
• VBCVPMMZEGZULK-NRFANRHFSA-N indoxacarb Chemical compound C([C@@]1(OC2)C(=O)OC)C3=CC(Cl)=CC=C3C1=NN2C(=O)N(C(=O)OC)C1=CC=C(OC(F)(F)F)C=C1 VBCVPMMZEGZULK-NRFANRHFSA-N 0.000 abstract 1
• 210000004027 cell Anatomy 0.000 description 152
• 235000018102 proteins Nutrition 0.000 description 83
• 108090000765 processed proteins & peptides Proteins 0.000 description 65
• 235000001014 amino acid Nutrition 0.000 description 50
• 229940024606 amino acid Drugs 0.000 description 49
• 102000004196 processed proteins & peptides Human genes 0.000 description 46
• 101000971171 Homo sapiens Apoptosis regulator Bcl-2 Proteins 0.000 description 41
• 229920001184 polypeptide Polymers 0.000 description 39
• 102100021569 Apoptosis regulator Bcl-2 Human genes 0.000 description 36
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 32
• 239000013612 plasmid Substances 0.000 description 27
• 239000002585 base Substances 0.000 description 23
• 210000001519 tissue Anatomy 0.000 description 23
• 238000004458 analytical method Methods 0.000 description 22
• 238000003752 polymerase chain reaction Methods 0.000 description 22
• 102000004039 Caspase-9 Human genes 0.000 description 21
• 108090000566 Caspase-9 Proteins 0.000 description 21
• 230000000694 effects Effects 0.000 description 21
• 230000003993 interaction Effects 0.000 description 21
• 239000002299 complementary DNA Substances 0.000 description 20
• 230000030833 cell death Effects 0.000 description 19
• 239000000047 product Substances 0.000 description 19
• 206010028980 Neoplasm Diseases 0.000 description 18
• 239000000243 solution Substances 0.000 description 16
• 238000013518 transcription Methods 0.000 description 16
• 230000035897 transcription Effects 0.000 description 16
• 108091034117 Oligonucleotide Proteins 0.000 description 15
• 239000003795 chemical substances by application Substances 0.000 description 15
• 230000006870 function Effects 0.000 description 15
• 230000002424 anti-apoptotic effect Effects 0.000 description 14
• 239000000284 extract Substances 0.000 description 14
• 239000008194 pharmaceutical composition Substances 0.000 description 14
• 238000010186 staining Methods 0.000 description 14
• 102000051485 Bcl-2 family Human genes 0.000 description 13
• 108700038897 Bcl-2 family Proteins 0.000 description 13
• 108020004705 Codon Proteins 0.000 description 13
• 241000699670 Mus sp. Species 0.000 description 13
• 241000710960 Sindbis virus Species 0.000 description 13
• 102000055102 bcl-2-Associated X Human genes 0.000 description 13
• 108700000707 bcl-2-Associated X Proteins 0.000 description 13
• 238000001727 in vivo Methods 0.000 description 13
• 238000003780 insertion Methods 0.000 description 13
• 230000037431 insertion Effects 0.000 description 13
• 239000012528 membrane Substances 0.000 description 13
• 230000014616 translation Effects 0.000 description 13
• 230000004913 activation Effects 0.000 description 12
• 238000003199 nucleic acid amplification method Methods 0.000 description 12
• 230000008569 process Effects 0.000 description 12
• 230000001225 therapeutic effect Effects 0.000 description 12
• 238000013519 translation Methods 0.000 description 12
• 230000003321 amplification Effects 0.000 description 11
• 238000010367 cloning Methods 0.000 description 11
• 238000000749 co-immunoprecipitation Methods 0.000 description 11
• 239000013615 primer Substances 0.000 description 11
• 238000000746 purification Methods 0.000 description 11
• 230000009385 viral infection Effects 0.000 description 11
• 108020004635 Complementary DNA Proteins 0.000 description 10
• 230000004075 alteration Effects 0.000 description 10
• 230000015572 biosynthetic process Effects 0.000 description 10
• 230000002759 chromosomal effect Effects 0.000 description 10
• 210000000349 chromosome Anatomy 0.000 description 10
• 238000003776 cleavage reaction Methods 0.000 description 10
• 238000000338 in vitro Methods 0.000 description 10
• 239000003550 marker Substances 0.000 description 10
• 230000007017 scission Effects 0.000 description 10
• 208000023275 Autoimmune disease Diseases 0.000 description 9
• 108020004511 Recombinant DNA Proteins 0.000 description 9
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 9
• 241000700605 Viruses Species 0.000 description 9
• 238000006243 chemical reaction Methods 0.000 description 9
• 239000003814 drug Substances 0.000 description 9
• 108020004999 messenger RNA Proteins 0.000 description 9
• 230000004048 modification Effects 0.000 description 9
• 238000012986 modification Methods 0.000 description 9
• 108091008146 restriction endonucleases Proteins 0.000 description 9
• 102000004190 Enzymes Human genes 0.000 description 8
• 108090000790 Enzymes Proteins 0.000 description 8
• 208000036142 Viral infection Diseases 0.000 description 8
• 238000007792 addition Methods 0.000 description 8
• 230000001640 apoptogenic effect Effects 0.000 description 8
• 108010005774 beta-Galactosidase Proteins 0.000 description 8
• 201000011510 cancer Diseases 0.000 description 8
• 239000013592 cell lysate Substances 0.000 description 8
• 230000006378 damage Effects 0.000 description 8
• 238000001514 detection method Methods 0.000 description 8
• 238000003745 diagnosis Methods 0.000 description 8
• 229940088598 enzyme Drugs 0.000 description 8
• 230000002068 genetic effect Effects 0.000 description 8
• 238000003786 synthesis reaction Methods 0.000 description 8
• 239000003981 vehicle Substances 0.000 description 8
• 208000030507 AIDS Diseases 0.000 description 7
• 102100026189 Beta-galactosidase Human genes 0.000 description 7
• 108090000994 Catalytic RNA Proteins 0.000 description 7
• 102000053642 Catalytic RNA Human genes 0.000 description 7
• 101100007328 Cocos nucifera COS-1 gene Proteins 0.000 description 7
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
• 108010038807 Oligopeptides Proteins 0.000 description 7
• 102000015636 Oligopeptides Human genes 0.000 description 7
• 241000283973 Oryctolagus cuniculus Species 0.000 description 7
• 108091081024 Start codon Proteins 0.000 description 7
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 7
• 230000001580 bacterial effect Effects 0.000 description 7
• 230000008859 change Effects 0.000 description 7
• 208000035475 disorder Diseases 0.000 description 7
• 238000002474 experimental method Methods 0.000 description 7
• 102000037865 fusion proteins Human genes 0.000 description 7
• 108020001507 fusion proteins Proteins 0.000 description 7
• 230000006698 induction Effects 0.000 description 7
• 230000007246 mechanism Effects 0.000 description 7
• 208000015122 neurodegenerative disease Diseases 0.000 description 7
• 238000011084 recovery Methods 0.000 description 7
• 230000001105 regulatory effect Effects 0.000 description 7
• 108091092562 ribozyme Proteins 0.000 description 7
• 239000007787 solid Substances 0.000 description 7
• 241000894007 species Species 0.000 description 7
• 239000000126 substance Substances 0.000 description 7
• 230000004083 survival effect Effects 0.000 description 7
• 239000000725 suspension Substances 0.000 description 7
• 238000011144 upstream manufacturing Methods 0.000 description 7
• 102000053602 DNA Human genes 0.000 description 6
• 241000588724 Escherichia coli Species 0.000 description 6
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
• 241000282412 Homo Species 0.000 description 6
• 239000000427 antigen Substances 0.000 description 6
• 108091007433 antigens Proteins 0.000 description 6
• 102000036639 antigens Human genes 0.000 description 6
• 238000010276 construction Methods 0.000 description 6
• 230000034994 death Effects 0.000 description 6
• 231100000517 death Toxicity 0.000 description 6
• 238000011161 development Methods 0.000 description 6
• 230000018109 developmental process Effects 0.000 description 6
• 239000000499 gel Substances 0.000 description 6
• 238000012744 immunostaining Methods 0.000 description 6
• 230000035772 mutation Effects 0.000 description 6
• 230000004770 neurodegeneration Effects 0.000 description 6
• 230000036961 partial effect Effects 0.000 description 6
• 238000002360 preparation method Methods 0.000 description 6
• 210000002966 serum Anatomy 0.000 description 6
• 230000005945 translocation Effects 0.000 description 6
• 238000010396 two-hybrid screening Methods 0.000 description 6
• 108700028369 Alleles Proteins 0.000 description 5
• 108091026890 Coding region Proteins 0.000 description 5
• 208000029462 Immunodeficiency disease Diseases 0.000 description 5
• 108010002386 Interleukin-3 Proteins 0.000 description 5
• 241000699666 Mus <mouse, genus> Species 0.000 description 5
• 108700026244 Open Reading Frames Proteins 0.000 description 5
• 238000010171 animal model Methods 0.000 description 5
• 230000000890 antigenic effect Effects 0.000 description 5
• 238000001574 biopsy Methods 0.000 description 5
• 230000001413 cellular effect Effects 0.000 description 5
• 210000004978 chinese hamster ovary cell Anatomy 0.000 description 5
• 238000012217 deletion Methods 0.000 description 5
• 230000037430 deletion Effects 0.000 description 5
• 239000008298 dragée Substances 0.000 description 5
• 229940079593 drug Drugs 0.000 description 5
• 238000005516 engineering process Methods 0.000 description 5
• 239000003623 enhancer Substances 0.000 description 5
• 238000002509 fluorescent in situ hybridization Methods 0.000 description 5
• 230000004927 fusion Effects 0.000 description 5
• 238000003018 immunoassay Methods 0.000 description 5
• 230000000302 ischemic effect Effects 0.000 description 5
• 239000006166 lysate Substances 0.000 description 5
• 239000002609 medium Substances 0.000 description 5
• 210000002569 neuron Anatomy 0.000 description 5
• 210000004940 nucleus Anatomy 0.000 description 5
• 238000012545 processing Methods 0.000 description 5
• 238000011002 quantification Methods 0.000 description 5
• 150000003839 salts Chemical class 0.000 description 5
• 238000012163 sequencing technique Methods 0.000 description 5
• 238000006467 substitution reaction Methods 0.000 description 5
• 238000012360 testing method Methods 0.000 description 5
• 241000701161 unidentified adenovirus Species 0.000 description 5
• 102000003952 Caspase 3 Human genes 0.000 description 4
• 108090000397 Caspase 3 Proteins 0.000 description 4
• 102000011727 Caspases Human genes 0.000 description 4
• 108010076667 Caspases Proteins 0.000 description 4
• 241000701489 Cauliflower mosaic virus Species 0.000 description 4
• 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 4
• 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 4
• 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 4
• 241000196324 Embryophyta Species 0.000 description 4
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
• 201000003793 Myelodysplastic syndrome Diseases 0.000 description 4
• 102000013535 Proto-Oncogene Proteins c-bcl-2 Human genes 0.000 description 4
• 108010090931 Proto-Oncogene Proteins c-bcl-2 Proteins 0.000 description 4
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
• 239000004480 active ingredient Substances 0.000 description 4
• 125000000539 amino acid group Chemical group 0.000 description 4
• 239000002775 capsule Substances 0.000 description 4
• 230000024245 cell differentiation Effects 0.000 description 4
• 230000004663 cell proliferation Effects 0.000 description 4
• 239000003153 chemical reaction reagent Substances 0.000 description 4
• 230000001086 cytosolic effect Effects 0.000 description 4
• SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 4
• SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 4
• 230000008030 elimination Effects 0.000 description 4
• 238000003379 elimination reaction Methods 0.000 description 4
• 230000002255 enzymatic effect Effects 0.000 description 4
• 238000001943 fluorescence-activated cell sorting Methods 0.000 description 4
• 238000009472 formulation Methods 0.000 description 4
• 239000003102 growth factor Substances 0.000 description 4
• UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 4
• 229910052739 hydrogen Inorganic materials 0.000 description 4
• 239000001257 hydrogen Substances 0.000 description 4
• 238000001114 immunoprecipitation Methods 0.000 description 4
• 239000003999 initiator Substances 0.000 description 4
• 239000007788 liquid Substances 0.000 description 4
• 210000004962 mammalian cell Anatomy 0.000 description 4
• 230000002018 overexpression Effects 0.000 description 4
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 4
• 230000000861 pro-apoptotic effect Effects 0.000 description 4
• 230000035755 proliferation Effects 0.000 description 4
• 230000002829 reductive effect Effects 0.000 description 4
• 230000010076 replication Effects 0.000 description 4
• 230000004044 response Effects 0.000 description 4
• 230000035945 sensitivity Effects 0.000 description 4
• 239000011780 sodium chloride Substances 0.000 description 4
• 239000000758 substrate Substances 0.000 description 4
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
• 201000001320 Atherosclerosis Diseases 0.000 description 3
• 102100024080 CASP8-associated protein 2 Human genes 0.000 description 3
• 102100035904 Caspase-1 Human genes 0.000 description 3
• 108090000426 Caspase-1 Proteins 0.000 description 3
• 102100030497 Cytochrome c Human genes 0.000 description 3
• 108010075031 Cytochromes c Proteins 0.000 description 3
• FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 3
• FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 3
• 108010013369 Enteropeptidase Proteins 0.000 description 3
• 102100029727 Enteropeptidase Human genes 0.000 description 3
• 108010010803 Gelatin Proteins 0.000 description 3
• HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 3
• 101000910382 Homo sapiens CASP8-associated protein 2 Proteins 0.000 description 3
• 108060003951 Immunoglobulin Proteins 0.000 description 3
• 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 3
• 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 3
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 3
• 208000030289 Lymphoproliferative disease Diseases 0.000 description 3
• 241001529936 Murinae Species 0.000 description 3
• 208000001132 Osteoporosis Diseases 0.000 description 3
• 239000002202 Polyethylene glycol Substances 0.000 description 3
• 241000700159 Rattus Species 0.000 description 3
• 108020005038 Terminator Codon Proteins 0.000 description 3
• 241000723873 Tobacco mosaic virus Species 0.000 description 3
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 3
• 239000002253 acid Substances 0.000 description 3
• 239000002246 antineoplastic agent Substances 0.000 description 3
• 239000000074 antisense oligonucleotide Substances 0.000 description 3
• 238000012230 antisense oligonucleotides Methods 0.000 description 3
• 210000003719 b-lymphocyte Anatomy 0.000 description 3
• 108700041737 bcl-2 Genes Proteins 0.000 description 3
• 230000008901 benefit Effects 0.000 description 3
• 230000033228 biological regulation Effects 0.000 description 3
• 210000001124 body fluid Anatomy 0.000 description 3
• 239000010839 body fluid Substances 0.000 description 3
• 150000001720 carbohydrates Chemical class 0.000 description 3
• 235000014633 carbohydrates Nutrition 0.000 description 3
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
• 101150055276 ced-3 gene Proteins 0.000 description 3
• 210000003169 central nervous system Anatomy 0.000 description 3
• 238000012761 co-transfection Methods 0.000 description 3
• 230000009918 complex formation Effects 0.000 description 3
• 230000000875 corresponding effect Effects 0.000 description 3
• 230000008878 coupling Effects 0.000 description 3
• 238000010168 coupling process Methods 0.000 description 3
• 238000005859 coupling reaction Methods 0.000 description 3
• 229940127089 cytotoxic agent Drugs 0.000 description 3
• 230000001419 dependent effect Effects 0.000 description 3
• 230000029087 digestion Effects 0.000 description 3
• 239000008273 gelatin Substances 0.000 description 3
• 229920000159 gelatin Polymers 0.000 description 3
• 235000019322 gelatine Nutrition 0.000 description 3
• 235000011852 gelatine desserts Nutrition 0.000 description 3
• 239000003862 glucocorticoid Substances 0.000 description 3
• 230000013595 glycosylation Effects 0.000 description 3
• 238000006206 glycosylation reaction Methods 0.000 description 3
• 210000003958 hematopoietic stem cell Anatomy 0.000 description 3
• 239000005556 hormone Substances 0.000 description 3
• 229940088597 hormone Drugs 0.000 description 3
• 230000001900 immune effect Effects 0.000 description 3
• 230000028993 immune response Effects 0.000 description 3
• 230000002163 immunogen Effects 0.000 description 3
• 102000018358 immunoglobulin Human genes 0.000 description 3
• 239000012133 immunoprecipitate Substances 0.000 description 3
• 238000011534 incubation Methods 0.000 description 3
• 230000001939 inductive effect Effects 0.000 description 3
• 208000015181 infectious disease Diseases 0.000 description 3
• 230000005764 inhibitory process Effects 0.000 description 3
• 229910052751 metal Inorganic materials 0.000 description 3
• 239000002184 metal Substances 0.000 description 3
• 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 3
• 230000009871 nonspecific binding Effects 0.000 description 3
• 229920001223 polyethylene glycol Polymers 0.000 description 3
• 238000001556 precipitation Methods 0.000 description 3
• 230000001681 protective effect Effects 0.000 description 3
• 238000003127 radioimmunoassay Methods 0.000 description 3
• 238000011160 research Methods 0.000 description 3
• 239000011347 resin Substances 0.000 description 3
• 229920005989 resin Polymers 0.000 description 3
• 230000028327 secretion Effects 0.000 description 3
• 239000007790 solid phase Substances 0.000 description 3
• 239000002904 solvent Substances 0.000 description 3
• 239000000600 sorbitol Substances 0.000 description 3
• 230000009870 specific binding Effects 0.000 description 3
• 239000003381 stabilizer Substances 0.000 description 3
• 239000003053 toxin Substances 0.000 description 3
• 231100000765 toxin Toxicity 0.000 description 3
• 108700012359 toxins Proteins 0.000 description 3
• 230000002103 transcriptional effect Effects 0.000 description 3
• 230000009466 transformation Effects 0.000 description 3
• 235000012431 wafers Nutrition 0.000 description 3
• YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
• MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 2
• 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
• 101100216424 African swine fever virus (strain E-70 MS44) LMW5-HL gene Proteins 0.000 description 2
• 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
• 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
• 208000024827 Alzheimer disease Diseases 0.000 description 2
• 108020000948 Antisense Oligonucleotides Proteins 0.000 description 2
• 208000032467 Aplastic anaemia Diseases 0.000 description 2
• 239000004475 Arginine Substances 0.000 description 2
• 108090001008 Avidin Proteins 0.000 description 2
• 241000894006 Bacteria Species 0.000 description 2
• 241000283690 Bos taurus Species 0.000 description 2
• 206010006187 Breast cancer Diseases 0.000 description 2
• 208000026310 Breast neoplasm Diseases 0.000 description 2
• 241000282472 Canis lupus familiaris Species 0.000 description 2
• 241000283707 Capra Species 0.000 description 2
• 102000014914 Carrier Proteins Human genes 0.000 description 2
• 108010078791 Carrier Proteins Proteins 0.000 description 2
• 241000701022 Cytomegalovirus Species 0.000 description 2
• FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
• 108010017826 DNA Polymerase I Proteins 0.000 description 2
• 102000004594 DNA Polymerase I Human genes 0.000 description 2
• 238000001712 DNA sequencing Methods 0.000 description 2
• 108010042407 Endonucleases Proteins 0.000 description 2
• 102000004533 Endonucleases Human genes 0.000 description 2
• 102100032670 Endophilin-B1 Human genes 0.000 description 2
• 108060002716 Exonuclease Proteins 0.000 description 2
• ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
• 239000004471 Glycine Substances 0.000 description 2
• 241000238631 Hexapoda Species 0.000 description 2
• 101000654648 Homo sapiens Endophilin-B1 Proteins 0.000 description 2
• 241000714260 Human T-lymphotropic virus 1 Species 0.000 description 2
• 241000701044 Human gammaherpesvirus 4 Species 0.000 description 2
• UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
• PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 2
• XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 2
• 102000008394 Immunoglobulin Fragments Human genes 0.000 description 2
• 108010021625 Immunoglobulin Fragments Proteins 0.000 description 2
• 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 description 2
• 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 description 2
• SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
• KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
• QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
• GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
• 108060001084 Luciferase Proteins 0.000 description 2
• 239000005089 Luciferase Substances 0.000 description 2
• 206010025323 Lymphomas Diseases 0.000 description 2
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
• 239000004472 Lysine Substances 0.000 description 2
• 229930195725 Mannitol Natural products 0.000 description 2
• 241000699660 Mus musculus Species 0.000 description 2
• 241001467552 Mycobacterium bovis BCG Species 0.000 description 2
• 206010061535 Ovarian neoplasm Diseases 0.000 description 2
• 238000012408 PCR amplification Methods 0.000 description 2
• 208000018737 Parkinson disease Diseases 0.000 description 2
• 241000237988 Patellidae Species 0.000 description 2
• ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
• 108091000080 Phosphotransferase Proteins 0.000 description 2
• 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 2
• 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 2
• 208000000236 Prostatic Neoplasms Diseases 0.000 description 2
• 108010076504 Protein Sorting Signals Proteins 0.000 description 2
• 102000052575 Proto-Oncogene Human genes 0.000 description 2
• 108700020978 Proto-Oncogene Proteins 0.000 description 2
• 108020004518 RNA Probes Proteins 0.000 description 2
• 239000003391 RNA probe Substances 0.000 description 2
• 108700005075 Regulator Genes Proteins 0.000 description 2
• 241000714474 Rous sarcoma virus Species 0.000 description 2
• 229920002684 Sepharose Polymers 0.000 description 2
• 238000012300 Sequence Analysis Methods 0.000 description 2
• 229920002472 Starch Polymers 0.000 description 2
• 208000006011 Stroke Diseases 0.000 description 2
• 229930006000 Sucrose Natural products 0.000 description 2
• CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
• 201000009594 Systemic Scleroderma Diseases 0.000 description 2
• 206010042953 Systemic sclerosis Diseases 0.000 description 2
• 208000024313 Testicular Neoplasms Diseases 0.000 description 2
• GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
• 108060008682 Tumor Necrosis Factor Proteins 0.000 description 2
• 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 2
• 238000009825 accumulation Methods 0.000 description 2
• DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 2
• 230000021736 acetylation Effects 0.000 description 2
• 238000006640 acetylation reaction Methods 0.000 description 2
• 230000003213 activating effect Effects 0.000 description 2
• 230000010933 acylation Effects 0.000 description 2
• 238000005917 acylation reaction Methods 0.000 description 2
• 239000002671 adjuvant Substances 0.000 description 2
• 235000004279 alanine Nutrition 0.000 description 2
• 125000003277 amino group Chemical group 0.000 description 2
• 206010002026 amyotrophic lateral sclerosis Diseases 0.000 description 2
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
• 206010003246 arthritis Diseases 0.000 description 2
• 210000001106 artificial yeast chromosome Anatomy 0.000 description 2
• 229960000190 bacillus calmette–guérin vaccine Drugs 0.000 description 2
• 108700000711 bcl-X Proteins 0.000 description 2
• 125000004057 biotinyl group Chemical group [H]N1C(=O)N([H])[C@]2([H])[C@@]([H])(SC([H])([H])[C@]12[H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C(*)=O 0.000 description 2
• 210000004556 brain Anatomy 0.000 description 2
• 210000000481 breast Anatomy 0.000 description 2
• AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
• 238000005251 capillar electrophoresis Methods 0.000 description 2
• 239000001768 carboxy methyl cellulose Substances 0.000 description 2
• 230000021523 carboxylation Effects 0.000 description 2
• 238000006473 carboxylation reaction Methods 0.000 description 2
• 230000010261 cell growth Effects 0.000 description 2
• 238000012875 competitive assay Methods 0.000 description 2
• 230000002596 correlated effect Effects 0.000 description 2
• OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
• 230000003247 decreasing effect Effects 0.000 description 2
• 238000000326 densiometry Methods 0.000 description 2
• 238000002405 diagnostic procedure Methods 0.000 description 2
• 230000004069 differentiation Effects 0.000 description 2
• 238000010790 dilution Methods 0.000 description 2
• 239000012895 dilution Substances 0.000 description 2
• PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 2
• 239000012636 effector Substances 0.000 description 2
• 238000001962 electrophoresis Methods 0.000 description 2
• 230000002616 endonucleolytic effect Effects 0.000 description 2
• 102000013165 exonuclease Human genes 0.000 description 2
• 239000010685 fatty oil Substances 0.000 description 2
• 239000000945 filler Substances 0.000 description 2
• 239000012530 fluid Substances 0.000 description 2
• 230000003325 follicular Effects 0.000 description 2
• 201000003444 follicular lymphoma Diseases 0.000 description 2
• 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 2
• 230000012010 growth Effects 0.000 description 2
• 230000036541 health Effects 0.000 description 2
• 108060003552 hemocyanin Proteins 0.000 description 2
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
• 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 2
• 210000004408 hybridoma Anatomy 0.000 description 2
• 230000002209 hydrophobic effect Effects 0.000 description 2
• 229960002591 hydroxyproline Drugs 0.000 description 2
• 238000007901 in situ hybridization Methods 0.000 description 2
• 238000010348 incorporation Methods 0.000 description 2
• 230000006882 induction of apoptosis Effects 0.000 description 2
• 230000028709 inflammatory response Effects 0.000 description 2
• 208000014674 injury Diseases 0.000 description 2
• 230000002452 interceptive effect Effects 0.000 description 2
• 210000003734 kidney Anatomy 0.000 description 2
• 238000002372 labelling Methods 0.000 description 2
• JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
• 235000014655 lactic acid Nutrition 0.000 description 2
• 239000008101 lactose Substances 0.000 description 2
• 235000005772 leucine Nutrition 0.000 description 2
• 208000032839 leukemia Diseases 0.000 description 2
• 230000029226 lipidation Effects 0.000 description 2
• HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
• 230000003211 malignant effect Effects 0.000 description 2
• 239000000594 mannitol Substances 0.000 description 2
• 235000010355 mannitol Nutrition 0.000 description 2
• 239000000463 material Substances 0.000 description 2
• 239000011159 matrix material Substances 0.000 description 2
• 238000002844 melting Methods 0.000 description 2
• 230000008018 melting Effects 0.000 description 2
• 230000031864 metaphase Effects 0.000 description 2
• 229930182817 methionine Natural products 0.000 description 2
• 238000002156 mixing Methods 0.000 description 2
• 208000010125 myocardial infarction Diseases 0.000 description 2
• 230000009826 neoplastic cell growth Effects 0.000 description 2
• 230000001613 neoplastic effect Effects 0.000 description 2
• 230000003472 neutralizing effect Effects 0.000 description 2
• 230000001575 pathological effect Effects 0.000 description 2
• 230000035515 penetration Effects 0.000 description 2
• 238000010647 peptide synthesis reaction Methods 0.000 description 2
• 239000008177 pharmaceutical agent Substances 0.000 description 2
• 239000000546 pharmaceutical excipient Substances 0.000 description 2
• 239000000825 pharmaceutical preparation Substances 0.000 description 2
• 230000026731 phosphorylation Effects 0.000 description 2
• 238000006366 phosphorylation reaction Methods 0.000 description 2
• 102000020233 phosphotransferase Human genes 0.000 description 2
• 239000004033 plastic Substances 0.000 description 2
• 229920003023 plastic Polymers 0.000 description 2
• 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
• 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
• 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
• 210000002307 prostate Anatomy 0.000 description 2
• 230000009993 protective function Effects 0.000 description 2
• 230000004850 protein–protein interaction Effects 0.000 description 2
• 230000005855 radiation Effects 0.000 description 2
• 239000011541 reaction mixture Substances 0.000 description 2
• 230000003362 replicative effect Effects 0.000 description 2
• 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 2
• 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 2
• 238000010561 standard procedure Methods 0.000 description 2
• 235000019698 starch Nutrition 0.000 description 2
• 239000005720 sucrose Substances 0.000 description 2
• 235000000346 sugar Nutrition 0.000 description 2
• 208000024891 symptom Diseases 0.000 description 2
• 229940037128 systemic glucocorticoids Drugs 0.000 description 2
• 201000000596 systemic lupus erythematosus Diseases 0.000 description 2
• 230000002381 testicular Effects 0.000 description 2
• 229940124597 therapeutic agent Drugs 0.000 description 2
• RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
• 230000000699 topical effect Effects 0.000 description 2
• 231100000419 toxicity Toxicity 0.000 description 2
• 230000001988 toxicity Effects 0.000 description 2
• 230000001131 transforming effect Effects 0.000 description 2
• 238000011830 transgenic mouse model Methods 0.000 description 2
• 230000001052 transient effect Effects 0.000 description 2
• 230000008733 trauma Effects 0.000 description 2
• 241001529453 unidentified herpesvirus Species 0.000 description 2
• 229960005486 vaccine Drugs 0.000 description 2
• 230000003612 virological effect Effects 0.000 description 2
• 238000001262 western blot Methods 0.000 description 2
• MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
• LNAZSHAWQACDHT-XIYTZBAFSA-N (2r,3r,4s,5r,6s)-4,5-dimethoxy-2-(methoxymethyl)-3-[(2s,3r,4s,5r,6r)-3,4,5-trimethoxy-6-(methoxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6r)-4,5,6-trimethoxy-2-(methoxymethyl)oxan-3-yl]oxyoxane Chemical compound CO[C@@H]1[C@@H](OC)[C@H](OC)[C@@H](COC)O[C@H]1O[C@H]1[C@H](OC)[C@@H](OC)[C@H](O[C@H]2[C@@H]([C@@H](OC)[C@H](OC)O[C@@H]2COC)OC)O[C@@H]1COC LNAZSHAWQACDHT-XIYTZBAFSA-N 0.000 description 1
• OCUSNPIJIZCRSZ-ZTZWCFDHSA-N (2s)-2-amino-3-methylbutanoic acid;(2s)-2-amino-4-methylpentanoic acid;(2s,3s)-2-amino-3-methylpentanoic acid Chemical compound CC(C)[C@H](N)C(O)=O.CC[C@H](C)[C@H](N)C(O)=O.CC(C)C[C@H](N)C(O)=O OCUSNPIJIZCRSZ-ZTZWCFDHSA-N 0.000 description 1
• ASWBNKHCZGQVJV-UHFFFAOYSA-N (3-hexadecanoyloxy-2-hydroxypropyl) 2-(trimethylazaniumyl)ethyl phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(O)COP([O-])(=O)OCC[N+](C)(C)C ASWBNKHCZGQVJV-UHFFFAOYSA-N 0.000 description 1
• 125000003088 (fluoren-9-ylmethoxy)carbonyl group Chemical group 0.000 description 1
• HOURAEXAZWBEAW-UHFFFAOYSA-N 1-aminopropane-1,1,3-tricarboxylic acid Chemical compound OC(=O)C(C(O)=O)(N)CCC(O)=O HOURAEXAZWBEAW-UHFFFAOYSA-N 0.000 description 1
• UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
• IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
• GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
• UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 description 1
• HFEMPWKWYHWPQX-UHFFFAOYSA-N 2-amino-3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]propanoic acid;hydrochloride Chemical compound Cl.IC1=CC(CC(N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 HFEMPWKWYHWPQX-UHFFFAOYSA-N 0.000 description 1
• 101710091601 21 kDa protein Proteins 0.000 description 1
• BRMWTNUJHUMWMS-UHFFFAOYSA-N 3-Methylhistidine Natural products CN1C=NC(CC(N)C(O)=O)=C1 BRMWTNUJHUMWMS-UHFFFAOYSA-N 0.000 description 1
• OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 1
• OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
• 229940117976 5-hydroxylysine Drugs 0.000 description 1
• 101150094949 APRT gene Proteins 0.000 description 1
• ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 1
• 244000215068 Acacia senegal Species 0.000 description 1
• 102000013563 Acid Phosphatase Human genes 0.000 description 1
• 108010051457 Acid Phosphatase Proteins 0.000 description 1
• 229930024421 Adenine Natural products 0.000 description 1
• GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
• 108010024223 Adenine phosphoribosyltransferase Proteins 0.000 description 1
• ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 1
• 241000701386 African swine fever virus Species 0.000 description 1
• 229920001817 Agar Polymers 0.000 description 1
• 229920000936 Agarose Polymers 0.000 description 1
• 108010025188 Alcohol oxidase Proteins 0.000 description 1
• GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
• 241000710929 Alphavirus Species 0.000 description 1
• 108091093088 Amplicon Proteins 0.000 description 1
• 108020004491 Antisense DNA Proteins 0.000 description 1
• 108020005544 Antisense RNA Proteins 0.000 description 1
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
• 241000416162 Astragalus gummifer Species 0.000 description 1
• 241001203868 Autographa californica Species 0.000 description 1
• 101150038469 Aven gene Proteins 0.000 description 1
• 208000003950 B-cell lymphoma Diseases 0.000 description 1
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
• 208000003174 Brain Neoplasms Diseases 0.000 description 1
• 241000244203 Caenorhabditis elegans Species 0.000 description 1
• 241000282693 Cercopithecidae Species 0.000 description 1
• 108700010070 Codon Usage Proteins 0.000 description 1
• 102000008186 Collagen Human genes 0.000 description 1
• 108010035532 Collagen Proteins 0.000 description 1
• 241000186216 Corynebacterium Species 0.000 description 1
• 241000699800 Cricetinae Species 0.000 description 1
• 241000699802 Cricetulus griseus Species 0.000 description 1
• UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
• IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
• 150000008574 D-amino acids Chemical class 0.000 description 1
• 101150074155 DHFR gene Proteins 0.000 description 1
• 102000012410 DNA Ligases Human genes 0.000 description 1
• 108010061982 DNA Ligases Proteins 0.000 description 1
• 239000003155 DNA primer Substances 0.000 description 1
• 230000004543 DNA replication Effects 0.000 description 1
• 241000450599 DNA viruses Species 0.000 description 1
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
• CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
• 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 1
• 108010053770 Deoxyribonucleases Proteins 0.000 description 1
• 102000016911 Deoxyribonucleases Human genes 0.000 description 1
• 229920002307 Dextran Polymers 0.000 description 1
• 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
• 241000255601 Drosophila melanogaster Species 0.000 description 1
• LVGKNOAMLMIIKO-UHFFFAOYSA-N Elaidinsaeure-aethylester Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC LVGKNOAMLMIIKO-UHFFFAOYSA-N 0.000 description 1
• 102100031780 Endonuclease Human genes 0.000 description 1
• 241000283086 Equidae Species 0.000 description 1
• 241000283073 Equus caballus Species 0.000 description 1
• 108091029865 Exogenous DNA Proteins 0.000 description 1
• 108700024394 Exon Proteins 0.000 description 1
• 206010073306 Exposure to radiation Diseases 0.000 description 1
• 108010074860 Factor Xa Proteins 0.000 description 1
• 241000282326 Felis catus Species 0.000 description 1
• BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
• 241000233866 Fungi Species 0.000 description 1
• 102100039556 Galectin-4 Human genes 0.000 description 1
• 241000287828 Gallus gallus Species 0.000 description 1
• 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
• 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
• 102000053187 Glucuronidase Human genes 0.000 description 1
• 108010060309 Glucuronidase Proteins 0.000 description 1
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
• 102000003886 Glycoproteins Human genes 0.000 description 1
• 108090000288 Glycoproteins Proteins 0.000 description 1
• 229920000084 Gum arabic Polymers 0.000 description 1
• 101000608765 Homo sapiens Galectin-4 Proteins 0.000 description 1
• 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
• 206010020843 Hyperthermia Diseases 0.000 description 1
• 206010020880 Hypertrophy Diseases 0.000 description 1
• 208000026350 Inborn Genetic disease Diseases 0.000 description 1
• 206010061218 Inflammation Diseases 0.000 description 1
• 108020005350 Initiator Codon Proteins 0.000 description 1
• 108091092195 Intron Proteins 0.000 description 1
• 108010025815 Kanamycin Kinase Proteins 0.000 description 1
• 208000007766 Kaposi sarcoma Diseases 0.000 description 1
• 208000008839 Kidney Neoplasms Diseases 0.000 description 1
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
• ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
• FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
• FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
• DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
• 239000006142 Luria-Bertani Agar Substances 0.000 description 1
• 206010064912 Malignant transformation Diseases 0.000 description 1
• 241000124008 Mammalia Species 0.000 description 1
• 101100261636 Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg) trpB2 gene Proteins 0.000 description 1
• 101710135898 Myc proto-oncogene protein Proteins 0.000 description 1
• 102100038895 Myc proto-oncogene protein Human genes 0.000 description 1
• JDHILDINMRGULE-LURJTMIESA-N N(pros)-methyl-L-histidine Chemical compound CN1C=NC=C1C[C@H](N)C(O)=O JDHILDINMRGULE-LURJTMIESA-N 0.000 description 1
• JJIHLJJYMXLCOY-BYPYZUCNSA-N N-acetyl-L-serine Chemical compound CC(=O)N[C@@H](CO)C(O)=O JJIHLJJYMXLCOY-BYPYZUCNSA-N 0.000 description 1
• PYUSHNKNPOHWEZ-YFKPBYRVSA-N N-formyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC=O PYUSHNKNPOHWEZ-YFKPBYRVSA-N 0.000 description 1
• 206010028851 Necrosis Diseases 0.000 description 1
• 241000244206 Nematoda Species 0.000 description 1
• 208000015914 Non-Hodgkin lymphomas Diseases 0.000 description 1
• 108010066154 Nuclear Export Signals Proteins 0.000 description 1
• 101710163270 Nuclease Proteins 0.000 description 1
• 108091005461 Nucleic proteins Proteins 0.000 description 1
• 108700020497 Nucleopolyhedrovirus polyhedrin Proteins 0.000 description 1
• 239000004677 Nylon Substances 0.000 description 1
• 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
• 240000007594 Oryza sativa Species 0.000 description 1
• 235000007164 Oryza sativa Nutrition 0.000 description 1
• 108090000854 Oxidoreductases Proteins 0.000 description 1
• 102000004316 Oxidoreductases Human genes 0.000 description 1
• 238000009004 PCR Kit Methods 0.000 description 1
• 101150012394 PHO5 gene Proteins 0.000 description 1
• 102000057297 Pepsin A Human genes 0.000 description 1
• 108090000284 Pepsin A Proteins 0.000 description 1
• 108010067902 Peptide Library Proteins 0.000 description 1
• 108091093037 Peptide nucleic acid Proteins 0.000 description 1
• IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
• 102000004861 Phosphoric Diester Hydrolases Human genes 0.000 description 1
• 101100124346 Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01) hisCD gene Proteins 0.000 description 1
• 230000007022 RNA scission Effects 0.000 description 1
• 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
• 102000006382 Ribonucleases Human genes 0.000 description 1
• 108010083644 Ribonucleases Proteins 0.000 description 1
• 108091028664 Ribonucleotide Proteins 0.000 description 1
• 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
• 229920005654 Sephadex Polymers 0.000 description 1
• 239000012507 Sephadex™ Substances 0.000 description 1
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
• 241000700584 Simplexvirus Species 0.000 description 1
• VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
• DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
• 229920002125 Sokalan® Polymers 0.000 description 1
• 244000061456 Solanum tuberosum Species 0.000 description 1
• 235000002595 Solanum tuberosum Nutrition 0.000 description 1
• 241000256251 Spodoptera frugiperda Species 0.000 description 1
• 108010090804 Streptavidin Proteins 0.000 description 1
• 241000187747 Streptomyces Species 0.000 description 1
• 241000282887 Suidae Species 0.000 description 1
• 108010006785 Taq Polymerase Proteins 0.000 description 1
• 241000255588 Tephritidae Species 0.000 description 1
• 102000002933 Thioredoxin Human genes 0.000 description 1
• 108010034949 Thyroglobulin Proteins 0.000 description 1
• 102000009843 Thyroglobulin Human genes 0.000 description 1
• 229920001615 Tragacanth Polymers 0.000 description 1
• 108700009124 Transcription Initiation Site Proteins 0.000 description 1
• 108091023040 Transcription factor Proteins 0.000 description 1
• 102000040945 Transcription factor Human genes 0.000 description 1
• 101710150448 Transcriptional regulator Myc Proteins 0.000 description 1
• 241000255985 Trichoplusia Species 0.000 description 1
• 235000021307 Triticum Nutrition 0.000 description 1
• 244000098338 Triticum aestivum Species 0.000 description 1
• GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
• 206010054094 Tumour necrosis Diseases 0.000 description 1
• 241000700647 Variola virus Species 0.000 description 1
• 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
• 108020005202 Viral DNA Proteins 0.000 description 1
• 108010067390 Viral Proteins Proteins 0.000 description 1
• IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 description 1
• 240000008042 Zea mays Species 0.000 description 1
• 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
• 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
• 230000001594 aberrant effect Effects 0.000 description 1
• 230000002159 abnormal effect Effects 0.000 description 1
• 238000002835 absorbance Methods 0.000 description 1
• 235000010489 acacia gum Nutrition 0.000 description 1
• 239000000205 acacia gum Substances 0.000 description 1
• 235000011054 acetic acid Nutrition 0.000 description 1
• 108020002494 acetyltransferase Proteins 0.000 description 1
• 102000005421 acetyltransferase Human genes 0.000 description 1
• 230000002378 acidificating effect Effects 0.000 description 1
• 150000007513 acids Chemical class 0.000 description 1
• 230000009471 action Effects 0.000 description 1
• 239000013543 active substance Substances 0.000 description 1
• 230000009692 acute damage Effects 0.000 description 1
• 125000002252 acyl group Chemical group 0.000 description 1
• 229960000643 adenine Drugs 0.000 description 1
• 230000001464 adherent effect Effects 0.000 description 1
• 230000002411 adverse Effects 0.000 description 1
• 238000001042 affinity chromatography Methods 0.000 description 1
• 238000001261 affinity purification Methods 0.000 description 1
• 239000008272 agar Substances 0.000 description 1
• 235000010419 agar Nutrition 0.000 description 1
• 239000011543 agarose gel Substances 0.000 description 1
• 238000011256 aggressive treatment Methods 0.000 description 1
• 235000010443 alginic acid Nutrition 0.000 description 1
• 239000000783 alginic acid Substances 0.000 description 1
• 229920000615 alginic acid Polymers 0.000 description 1
• 229960001126 alginic acid Drugs 0.000 description 1
• 150000004781 alginic acids Chemical class 0.000 description 1
• 125000000217 alkyl group Chemical group 0.000 description 1
• WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
• 210000004102 animal cell Anatomy 0.000 description 1
• 239000004410 anthocyanin Substances 0.000 description 1
• 229930002877 anthocyanin Natural products 0.000 description 1
• 235000010208 anthocyanin Nutrition 0.000 description 1
• 150000004636 anthocyanins Chemical class 0.000 description 1
• 230000001093 anti-cancer Effects 0.000 description 1
• 230000000340 anti-metabolite Effects 0.000 description 1
• 230000002788 anti-peptide Effects 0.000 description 1
• 229940100197 antimetabolite Drugs 0.000 description 1
• 239000002256 antimetabolite Substances 0.000 description 1
• 239000003816 antisense DNA Substances 0.000 description 1
• 238000013459 approach Methods 0.000 description 1
• 239000007864 aqueous solution Substances 0.000 description 1
• 239000003125 aqueous solvent Substances 0.000 description 1
• 210000001367 artery Anatomy 0.000 description 1
• 210000004436 artificial bacterial chromosome Anatomy 0.000 description 1
• 210000004507 artificial chromosome Anatomy 0.000 description 1
• 235000009582 asparagine Nutrition 0.000 description 1
• 229960001230 asparagine Drugs 0.000 description 1
• 235000003704 aspartic acid Nutrition 0.000 description 1
• 208000037979 autoimmune inflammatory disease Diseases 0.000 description 1
• 230000005784 autoimmunity Effects 0.000 description 1
• 238000000376 autoradiography Methods 0.000 description 1
• 108010058966 bacteriophage T7 induced DNA polymerase Proteins 0.000 description 1
• 230000004888 barrier function Effects 0.000 description 1
• 230000009286 beneficial effect Effects 0.000 description 1
• WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 1
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
• 239000011230 binding agent Substances 0.000 description 1
• 238000004166 bioassay Methods 0.000 description 1
• 230000003115 biocidal effect Effects 0.000 description 1
• 229960002685 biotin Drugs 0.000 description 1
• 235000020958 biotin Nutrition 0.000 description 1
• 239000011616 biotin Substances 0.000 description 1
• 238000007413 biotinylation Methods 0.000 description 1
• 230000006287 biotinylation Effects 0.000 description 1
• 230000000903 blocking effect Effects 0.000 description 1
• 229940098773 bovine serum albumin Drugs 0.000 description 1
• 210000004899 c-terminal region Anatomy 0.000 description 1
• 208000035269 cancer or benign tumor Diseases 0.000 description 1
• FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 1
• 229960003669 carbenicillin Drugs 0.000 description 1
• JJWKPURADFRFRB-UHFFFAOYSA-N carbonyl sulfide Chemical compound O=C=S JJWKPURADFRFRB-UHFFFAOYSA-N 0.000 description 1
• 239000000969 carrier Substances 0.000 description 1
• 230000003197 catalytic effect Effects 0.000 description 1
• 230000032823 cell division Effects 0.000 description 1
• 230000009134 cell regulation Effects 0.000 description 1
• 230000019522 cellular metabolic process Effects 0.000 description 1
• 239000001913 cellulose Substances 0.000 description 1
• 229920002678 cellulose Polymers 0.000 description 1
• 238000007385 chemical modification Methods 0.000 description 1
• VJYIFXVZLXQVHO-UHFFFAOYSA-N chlorsulfuron Chemical compound COC1=NC(C)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)Cl)=N1 VJYIFXVZLXQVHO-UHFFFAOYSA-N 0.000 description 1
• 238000004587 chromatography analysis Methods 0.000 description 1
• 238000003200 chromosome mapping Methods 0.000 description 1
• 239000013599 cloning vector Substances 0.000 description 1
• 238000000576 coating method Methods 0.000 description 1
• 229920001436 collagen Polymers 0.000 description 1
• 238000002648 combination therapy Methods 0.000 description 1
• 230000002301 combined effect Effects 0.000 description 1
• 230000009137 competitive binding Effects 0.000 description 1
• 230000002860 competitive effect Effects 0.000 description 1
• 239000003184 complementary RNA Substances 0.000 description 1
• 238000004590 computer program Methods 0.000 description 1
• 230000021615 conjugation Effects 0.000 description 1
• 239000013068 control sample Substances 0.000 description 1
• 238000011254 conventional chemotherapy Methods 0.000 description 1
• 238000001816 cooling Methods 0.000 description 1
• 235000005822 corn Nutrition 0.000 description 1
• 238000012937 correction Methods 0.000 description 1
• UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
• 230000009089 cytolysis Effects 0.000 description 1
• 210000000805 cytoplasm Anatomy 0.000 description 1
• 229940104302 cytosine Drugs 0.000 description 1
• 210000000172 cytosol Anatomy 0.000 description 1
• 230000002950 deficient Effects 0.000 description 1
• 238000006731 degradation reaction Methods 0.000 description 1
• 238000002716 delivery method Methods 0.000 description 1
• YSMODUONRAFBET-UHFFFAOYSA-N delta-DL-hydroxylysine Natural products NCC(O)CCC(N)C(O)=O YSMODUONRAFBET-UHFFFAOYSA-N 0.000 description 1
• 238000004925 denaturation Methods 0.000 description 1
• 230000036425 denaturation Effects 0.000 description 1
• 239000003599 detergent Substances 0.000 description 1
• 229960002086 dextran Drugs 0.000 description 1
• 229960000633 dextran sulfate Drugs 0.000 description 1
• 239000008121 dextrose Substances 0.000 description 1
• 235000005911 diet Nutrition 0.000 description 1
• 230000037213 diet Effects 0.000 description 1
• 235000014113 dietary fatty acids Nutrition 0.000 description 1
• 238000006471 dimerization reaction Methods 0.000 description 1
• 208000037765 diseases and disorders Diseases 0.000 description 1
• 239000012153 distilled water Substances 0.000 description 1
• 239000002552 dosage form Substances 0.000 description 1
• 239000000890 drug combination Substances 0.000 description 1
• 239000000975 dye Substances 0.000 description 1
• 238000004520 electroporation Methods 0.000 description 1
• 230000003028 elevating effect Effects 0.000 description 1
• 230000008451 emotion Effects 0.000 description 1
• 230000001804 emulsifying effect Effects 0.000 description 1
• 239000000839 emulsion Substances 0.000 description 1
• YSMODUONRAFBET-UHNVWZDZSA-N erythro-5-hydroxy-L-lysine Chemical compound NC[C@H](O)CC[C@H](N)C(O)=O YSMODUONRAFBET-UHNVWZDZSA-N 0.000 description 1
• LVGKNOAMLMIIKO-QXMHVHEDSA-N ethyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC LVGKNOAMLMIIKO-QXMHVHEDSA-N 0.000 description 1
• 229940093471 ethyl oleate Drugs 0.000 description 1
• 210000003527 eukaryotic cell Anatomy 0.000 description 1
• 230000007717 exclusion Effects 0.000 description 1
• 230000001747 exhibiting effect Effects 0.000 description 1
• 238000000605 extraction Methods 0.000 description 1
• 229930195729 fatty acid Natural products 0.000 description 1
• 239000000194 fatty acid Substances 0.000 description 1
• 238000000855 fermentation Methods 0.000 description 1
• 230000004151 fermentation Effects 0.000 description 1
• MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
• 239000007850 fluorescent dye Substances 0.000 description 1
• 238000006062 fragmentation reaction Methods 0.000 description 1
• 239000012458 free base Substances 0.000 description 1
• 208000016361 genetic disease Diseases 0.000 description 1
• 239000011521 glass Substances 0.000 description 1
• IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 1
• 235000013922 glutamic acid Nutrition 0.000 description 1
• 239000004220 glutamic acid Substances 0.000 description 1
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
• 102000005396 glutamine synthetase Human genes 0.000 description 1
• 108020002326 glutamine synthetase Proteins 0.000 description 1
• 239000008187 granular material Substances 0.000 description 1
• 238000000227 grinding Methods 0.000 description 1
• 239000001963 growth medium Substances 0.000 description 1
• 239000003630 growth substance Substances 0.000 description 1
• ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
• 238000010438 heat treatment Methods 0.000 description 1
• 230000002363 herbicidal effect Effects 0.000 description 1
• 239000004009 herbicide Substances 0.000 description 1
• 238000005734 heterodimerization reaction Methods 0.000 description 1
• 238000004128 high performance liquid chromatography Methods 0.000 description 1
• 101150113423 hisD gene Proteins 0.000 description 1
• 230000013632 homeostatic process Effects 0.000 description 1
• 210000000688 human artificial chromosome Anatomy 0.000 description 1
• 210000003917 human chromosome Anatomy 0.000 description 1
• 239000001866 hydroxypropyl methyl cellulose Substances 0.000 description 1
• 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 1
• 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 1
• UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 description 1
• 206010020718 hyperplasia Diseases 0.000 description 1
• 230000036031 hyperthermia Effects 0.000 description 1
• 230000003053 immunization Effects 0.000 description 1
• 238000002649 immunization Methods 0.000 description 1
• 230000005847 immunogenicity Effects 0.000 description 1
• 239000003547 immunosorbent Substances 0.000 description 1
• 238000012606 in vitro cell culture Methods 0.000 description 1
• 238000010874 in vitro model Methods 0.000 description 1
• PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
• RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
• 230000004054 inflammatory process Effects 0.000 description 1
• 230000000977 initiatory effect Effects 0.000 description 1
• 238000011081 inoculation Methods 0.000 description 1
• 229910001410 inorganic ion Inorganic materials 0.000 description 1
• 229910052500 inorganic mineral Inorganic materials 0.000 description 1
• 230000010354 integration Effects 0.000 description 1
• 108010027775 interleukin-1beta-converting enzyme inhibitor Proteins 0.000 description 1
• 238000001361 intraarterial administration Methods 0.000 description 1
• 230000003834 intracellular effect Effects 0.000 description 1
• 238000007918 intramuscular administration Methods 0.000 description 1
• 238000007912 intraperitoneal administration Methods 0.000 description 1
• 238000007913 intrathecal administration Methods 0.000 description 1
• 238000001990 intravenous administration Methods 0.000 description 1
• 238000007914 intraventricular administration Methods 0.000 description 1
• 238000007852 inverse PCR Methods 0.000 description 1
• 238000011835 investigation Methods 0.000 description 1
• 150000002500 ions Chemical class 0.000 description 1
• 238000002955 isolation Methods 0.000 description 1
• 229960000310 isoleucine Drugs 0.000 description 1
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
• FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 description 1
• BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
• 238000005304 joining Methods 0.000 description 1
• 108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 1
• 238000011005 laboratory method Methods 0.000 description 1
• 101150066555 lacZ gene Proteins 0.000 description 1
• 239000004922 lacquer Substances 0.000 description 1
• 239000004310 lactic acid Substances 0.000 description 1
• 229910052747 lanthanoid Inorganic materials 0.000 description 1
• 150000002602 lanthanoids Chemical class 0.000 description 1
• 231100000636 lethal dose Toxicity 0.000 description 1
• 150000002614 leucines Chemical class 0.000 description 1
• 230000000670 limiting effect Effects 0.000 description 1
• 150000002632 lipids Chemical class 0.000 description 1
• 238000001638 lipofection Methods 0.000 description 1
• 238000011068 loading method Methods 0.000 description 1
• 238000002865 local sequence alignment Methods 0.000 description 1
• 230000007774 longterm Effects 0.000 description 1
• 239000000314 lubricant Substances 0.000 description 1
• 210000004698 lymphocyte Anatomy 0.000 description 1
• 230000001589 lymphoproliferative effect Effects 0.000 description 1
• 239000008176 lyophilized powder Substances 0.000 description 1
• 210000002540 macrophage Anatomy 0.000 description 1
• 235000019359 magnesium stearate Nutrition 0.000 description 1
• 239000006249 magnetic particle Substances 0.000 description 1
• 235000011090 malic acid Nutrition 0.000 description 1
• 230000036212 malign transformation Effects 0.000 description 1
• 238000013507 mapping Methods 0.000 description 1
• 230000000873 masking effect Effects 0.000 description 1
• 230000035800 maturation Effects 0.000 description 1
• 238000005259 measurement Methods 0.000 description 1
• 230000010534 mechanism of action Effects 0.000 description 1
• 229910021645 metal ion Inorganic materials 0.000 description 1
• 150000002739 metals Chemical class 0.000 description 1
• 229960000485 methotrexate Drugs 0.000 description 1
• 229920000609 methyl cellulose Polymers 0.000 description 1
• 239000001923 methylcellulose Substances 0.000 description 1
• 235000010981 methylcellulose Nutrition 0.000 description 1
• 238000000386 microscopy Methods 0.000 description 1
• 230000005012 migration Effects 0.000 description 1
• 238000013508 migration Methods 0.000 description 1
• 230000003278 mimic effect Effects 0.000 description 1
• 239000011707 mineral Substances 0.000 description 1
• ZAHQPTJLOCWVPG-UHFFFAOYSA-N mitoxantrone dihydrochloride Chemical compound Cl.Cl.O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO ZAHQPTJLOCWVPG-UHFFFAOYSA-N 0.000 description 1
• 238000001823 molecular biology technique Methods 0.000 description 1
• 238000000329 molecular dynamics simulation Methods 0.000 description 1
• 230000009456 molecular mechanism Effects 0.000 description 1
• 239000000178 monomer Substances 0.000 description 1
• 230000000877 morphologic effect Effects 0.000 description 1
• 230000004660 morphological change Effects 0.000 description 1
• 230000000869 mutational effect Effects 0.000 description 1
• 208000025113 myeloid leukemia Diseases 0.000 description 1
• 208000031225 myocardial ischemia Diseases 0.000 description 1
• 230000017074 necrotic cell death Effects 0.000 description 1
• 229960004927 neomycin Drugs 0.000 description 1
• 210000005170 neoplastic cell Anatomy 0.000 description 1
• 230000001537 neural effect Effects 0.000 description 1
• 230000000508 neurotrophic effect Effects 0.000 description 1
• 230000002276 neurotropic effect Effects 0.000 description 1
• 238000006386 neutralization reaction Methods 0.000 description 1
• 231100000956 nontoxicity Toxicity 0.000 description 1
• 230000009701 normal cell proliferation Effects 0.000 description 1
• 230000008689 nuclear function Effects 0.000 description 1
• 230000031787 nutrient reservoir activity Effects 0.000 description 1
• 229920001778 nylon Polymers 0.000 description 1
• 239000003921 oil Substances 0.000 description 1
• 235000019198 oils Nutrition 0.000 description 1
• 239000002751 oligonucleotide probe Substances 0.000 description 1
• 230000006548 oncogenic transformation Effects 0.000 description 1
• 238000011275 oncology therapy Methods 0.000 description 1
• 230000003287 optical effect Effects 0.000 description 1
• 210000000056 organ Anatomy 0.000 description 1
• 239000003960 organic solvent Substances 0.000 description 1
• 210000001672 ovary Anatomy 0.000 description 1
• 238000007911 parenteral administration Methods 0.000 description 1
• 239000002245 particle Substances 0.000 description 1
• 230000008506 pathogenesis Effects 0.000 description 1
• 239000008188 pellet Substances 0.000 description 1
• 229940111202 pepsin Drugs 0.000 description 1
• 239000002831 pharmacologic agent Substances 0.000 description 1
• 239000012071 phase Substances 0.000 description 1
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
• PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 description 1
• 239000002644 phorbol ester Substances 0.000 description 1
• 125000003012 phosphonothioyl group Chemical group [H]P(*)(*)=S 0.000 description 1
• 150000008300 phosphoramidites Chemical class 0.000 description 1
• BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 description 1
• 230000035479 physiological effects, processes and functions Effects 0.000 description 1
• 239000000049 pigment Substances 0.000 description 1
• 239000006187 pill Substances 0.000 description 1
• 229920001983 poloxamer Polymers 0.000 description 1
• 229920000447 polyanionic polymer Polymers 0.000 description 1
• 229920000642 polymer Polymers 0.000 description 1
• 229920005862 polyol Polymers 0.000 description 1
• 150000003077 polyols Chemical class 0.000 description 1
• 235000012015 potatoes Nutrition 0.000 description 1
• 230000003389 potentiating effect Effects 0.000 description 1
• 230000003449 preventive effect Effects 0.000 description 1
• 230000001686 pro-survival effect Effects 0.000 description 1
• 210000001236 prokaryotic cell Anatomy 0.000 description 1
• 230000000644 propagated effect Effects 0.000 description 1
• 230000000069 prophylactic effect Effects 0.000 description 1
• 230000004952 protein activity Effects 0.000 description 1
• 238000001243 protein synthesis Methods 0.000 description 1
• 208000009305 pseudorabies Diseases 0.000 description 1
• 238000004445 quantitative analysis Methods 0.000 description 1
• 238000000163 radioactive labelling Methods 0.000 description 1
• 230000009467 reduction Effects 0.000 description 1
• 230000020129 regulation of cell death Effects 0.000 description 1
• 230000018866 regulation of programmed cell death Effects 0.000 description 1
• 230000008439 repair process Effects 0.000 description 1
• 125000006853 reporter group Chemical group 0.000 description 1
• 230000001850 reproductive effect Effects 0.000 description 1
• 230000000284 resting effect Effects 0.000 description 1
• 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
• 230000000717 retained effect Effects 0.000 description 1
• 230000002441 reversible effect Effects 0.000 description 1
• PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
• 239000002336 ribonucleotide Substances 0.000 description 1
• 125000002652 ribonucleotide group Chemical group 0.000 description 1
• 210000003705 ribosome Anatomy 0.000 description 1
• 235000009566 rice Nutrition 0.000 description 1
• 229920002477 rna polymer Polymers 0.000 description 1
• 238000010845 search algorithm Methods 0.000 description 1
• 239000006152 selective media Substances 0.000 description 1
• 238000000926 separation method Methods 0.000 description 1
• 239000008159 sesame oil Substances 0.000 description 1
• 235000011803 sesame oil Nutrition 0.000 description 1
• 230000035939 shock Effects 0.000 description 1
• 238000002741 site-directed mutagenesis Methods 0.000 description 1
• 239000002002 slurry Substances 0.000 description 1
• 150000003384 small molecules Chemical class 0.000 description 1
• 239000001632 sodium acetate Substances 0.000 description 1
• 235000017281 sodium acetate Nutrition 0.000 description 1
• 235000010413 sodium alginate Nutrition 0.000 description 1
• 239000000661 sodium alginate Substances 0.000 description 1
• 229940005550 sodium alginate Drugs 0.000 description 1
• 239000001509 sodium citrate Substances 0.000 description 1
• NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
• 239000007901 soft capsule Substances 0.000 description 1
• 239000012439 solid excipient Substances 0.000 description 1
• 238000005063 solubilization Methods 0.000 description 1
• 230000007928 solubilization Effects 0.000 description 1
• 125000006850 spacer group Chemical group 0.000 description 1
• 230000006641 stabilisation Effects 0.000 description 1
• 238000011105 stabilization Methods 0.000 description 1
• 230000010473 stable expression Effects 0.000 description 1
• 239000008107 starch Substances 0.000 description 1
• 210000000130 stem cell Anatomy 0.000 description 1
• 238000007920 subcutaneous administration Methods 0.000 description 1
• 235000011044 succinic acid Nutrition 0.000 description 1
• 150000003444 succinic acids Chemical class 0.000 description 1
• 150000008163 sugars Chemical class 0.000 description 1
• 125000000446 sulfanediyl group Chemical group *S* 0.000 description 1
• 230000001629 suppression Effects 0.000 description 1
• 208000011580 syndromic disease Diseases 0.000 description 1
• 230000002194 synthesizing effect Effects 0.000 description 1
• 239000006188 syrup Substances 0.000 description 1
• 235000020357 syrup Nutrition 0.000 description 1
• 239000003826 tablet Substances 0.000 description 1
• 239000000454 talc Substances 0.000 description 1
• 229910052623 talc Inorganic materials 0.000 description 1
• 235000012222 talc Nutrition 0.000 description 1
• 235000002906 tartaric acid Nutrition 0.000 description 1
• 231100001274 therapeutic index Toxicity 0.000 description 1
• HRGJTHLOGGFLIN-UHFFFAOYSA-N thiocyanic acid;urea Chemical compound SC#N.NC(N)=O HRGJTHLOGGFLIN-UHFFFAOYSA-N 0.000 description 1
• RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
• 108060008226 thioredoxin Proteins 0.000 description 1
• 229940094937 thioredoxin Drugs 0.000 description 1
• 229940113082 thymine Drugs 0.000 description 1
• 229960002175 thyroglobulin Drugs 0.000 description 1
• 238000012090 tissue culture technique Methods 0.000 description 1
• 239000004408 titanium dioxide Substances 0.000 description 1
• 231100000331 toxic Toxicity 0.000 description 1
• 230000002588 toxic effect Effects 0.000 description 1
• 235000010487 tragacanth Nutrition 0.000 description 1
• 239000000196 tragacanth Substances 0.000 description 1
• 229940116362 tragacanth Drugs 0.000 description 1
• 238000012546 transfer Methods 0.000 description 1
• 238000012301 transgenic model Methods 0.000 description 1
• 230000010474 transient expression Effects 0.000 description 1
• 230000014621 translational initiation Effects 0.000 description 1
• 238000002054 transplantation Methods 0.000 description 1
• 238000011269 treatment regimen Methods 0.000 description 1
• 150000003626 triacylglycerols Chemical class 0.000 description 1
• 239000001226 triphosphate Substances 0.000 description 1
• 101150081616 trpB gene Proteins 0.000 description 1
• 101150111232 trpB-1 gene Proteins 0.000 description 1
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
• 230000034512 ubiquitination Effects 0.000 description 1
• 238000010798 ubiquitination Methods 0.000 description 1
• 241000701447 unidentified baculovirus Species 0.000 description 1
• 241000701366 unidentified nuclear polyhedrosis viruses Species 0.000 description 1
• 241001515965 unidentified phage Species 0.000 description 1
• 241001430294 unidentified retrovirus Species 0.000 description 1
• 230000003827 upregulation Effects 0.000 description 1
• 238000012795 verification Methods 0.000 description 1
• 238000005406 washing Methods 0.000 description 1
• 230000029663 wound healing Effects 0.000 description 1