MXPA00006525A - Microorganism, method for obtaining same and feed additive - Google Patents

Abstract

The invention relates to a microorganism of the genus Eubacterium, and to its preparation and use. As a pure culture, DSM 11798, and/or as a mixed culture with the strain Enterococcus casseliflavus, DSM 11799, or as a mixed culture with other anaerobic microorganisms said microorganism is suitable for the detoxification of trichothecenes. The invention also relates to a feed additive for inactivating trichothecenes in feed or in the digestive system of animals, containing a pure and/or mixed culture of the microorganism (DSM 11798 or DSM 11799) or a mixed culture with other anaerobic microorganisms at a quantity of between 0.2 and 3 kg, especially between 0.5 and 2.5 kg, per 1,000 kg of feed.

Description

MICROORGANISM, PROCEDURE FOR OBTAINING IT AND ADDITIVE FOR FOOD The present invention relates to a microorganism of the genus Eubacteriales, which is suitable in pure culture or mixed culture for detoxification by trichothecenes, and with a process for the isolation thereof, its production, formulation and its use as well as an additive for food comprising the microorganism. Trichothecenes belonging to the class of mycotoxins are contained in numerous food products for animals, where these are customarily introduced into food products through mold fungi located in cereals or fats. As a result of the unwanted administration of the mycotoxins, in particular of trichothecenes, to the animals, it inhibits both their productivity, as, for example, the growth of the animals, an increase in the consumption of the feed together with a poorer utilization rate of the food is presented in addition to the damage to the health of the animals. To eliminate these adverse effects of mycotoxins, numerous methods for binding or absorbing these toxins have already been revealed. Thus, in WO 91/13555, for example, an additive is described for a food and a method for the inactivation of the mycotoxin, where the particles of a phyllosilicate mineral are added to the food in order to inactivate the mycotoxins. To increase the effect of these phyllosilicates, the particles are coated with an insulating agent in order to accelerate the effect. In addition, a food is known, for example, in WO 92/05706 in which montmorillonite clay is contained as an additive for food. These natural clay minerals that have large internal surface areas must bind the mycotoxins to the surface due to their porosity and immobilize them in this way. Furthermore, an additive for food has been revealed in the Austrian Utility Model AT-U 504 in which the preparation of an enzyme that has the ability to form epoxidases and lactonases and chemically degrade mycotoxins both in food and the gastrointestinal tract of animals. According to the AT-U 504, the action of this enzyme preparation can be increased through the addition of zeolites and the like. The present invention has the purpose of making available a specific microorganism or a defined mixed culture isolated from a natural habitat, with which it is possible to convert the mycotoxins, in particular the trichothecenes, in a controlled manner into substances that are not physiologically harmful and which are not harmful in particular in animals in reproduction, through a biochemical degradation.

To solve this objective, a microorganism of the genus Eubacteriales was isolated which is suitable for detoxification of the trichothecenes in pure culture DSM 11798, or in mixed culture with the chain of Enterococo casselifiavus, DSM 11799, or other anaerobic microorganisms. According to an innovative refinement of the invention, the microorganism is suitable for the detoxification of trichothecenes in mixed culture with other anaerobic microorganisms, in particular of the genus Enterococcus, Streptococcus, Lactococcus, Bacillus or Lactobacillus. The microorganism of the genus Eubacteriales, which is also called Eubacteriales sp. due to its association with the genus Eubacteriales, and which was deposited in the pure culture in the Collection of German Microorganisms under the number DSM 11798, or in mixed culture with the Enterococcus casselifiavus strain that was deposited in the Collection of the German Microorganism under the No. DSM 11799, is in particular suitable according to the invention for detoxification, in particular, deoxynivalenol (DON), T-2 toxin, nivalenol, monoacetoxiscirpenol, diacetoxyscirpenol, trichodermol, verrucarin, rorodine, acetyldeoxynivalenol, isotrichdermin, hydroxyisotrichdermin, calonectrin, tetraol T-2, Triol T-2, deacetilneosolaniol, tricotriol, sambucinol, and culmorina. The microorganisms according to the invention detoxify the trichothecenes through a reductive biotransformation of the epoxide group contained in the molecule, whose epoxide group is responsible for the toxicity of the mycotoxins, in particular the trichothecenes. In trichothecenes corresponding to the following formula, the degradation of the epoxide group is carried out through the reductive division of the toxic epoxy ring -12,13: Tricotecene Non-toxic reduced form The morphology of the microorganism according to the invention preferably shows that it is a gram-positive, rod-like anaerobic bacterium that does not form spores, in particular from 0.1 to 3 μm in length, which occurs individually, in pairs or in long chains, in particular up to approximately 150 μm. The phylogenetic analysis of the microorganism according to the invention has demonstrated in particular a 16S RNA sequence, namely: 1 CCTGGCTCAG GATGAACGCT GGCGGCGTGC TTAACACATG CAAGTCGAAC GGATAACCCG 61 CCTCCGGGCG GTTATAGAGT GGCGAACGGG TGAGTAACAC GTGACCAACC TACCTCCCAC 121 TCCGGGATAA CCCAGGGAAA CCTGCGCTAA TACCGGATAC TCCGGGGGCC CCGCATGGGG 181 GCGCCGGGAA AGCCCCGACG GTGGGAGATG GGGTCGCGGC CTATTAGGTA GTCGGCGGGG 241 TAACGGCCCC CCGAGCCCGC GATAGGTAGC CGGGTTGAGA GACCGATCGG CCACATTGGG 301 ACTGAGATAC GGCCCAGACT CCTACGGGAG GCAGCAGTGG GGAATTTTGC GCAATGGGGG 361 AAACCCTGAC GCAGCAACGC CGCGTGCGGG ACGAAGGCCT TCGGGTTGTA AACCGCTTTC 421 TACCTGCAGA AGCAGGGAAG AAGTTGACGG AGAAGCTCCG GCTAACTACG TGCCAGCAGC 481 CGCGGTAATA CGTAGGGAGC GAGCGTTATC CGGATTTATT GGGCGTAAAG CGCGCGTAGG 541 CGGGCGCTTA AGCGGAATCT CTAATCTGAG GGCTCAACCC CCAGCCGGAT TCCGAACTGG 601 GCGCCTCGAG TTCGGTAGAG GAAGACGGAA TTCCCAGTGT AGCGGTGAAA TGCGCAGATA 661 TTGGGAAGAA CACCGATGGC GAAGGCAGTC TTCTGGGCCG TAACTGACGC TGAGGTGCGA 721 AAGCTAGGGG AGCGAACAGG ATTAGATACC CTGGTAGTCC TAGCCGTAAA CGATGGGCAC 781 TAGGTGTGGG GGGGAATGCC CCTCCGTGCC GCAGCTAACG CATTAAGTGC CCCGCCTGGG 841 GAGTACGGCC GCAAGGCTAA AACTCAAAGG AATTGACCGG GGCCCGCACA AGCAGCGGAG 901 CATGTGGCTT AATTCGAAGC AACGCGAAGA ACCTTACCAG GGCTTGACAT GCAGGTGAAG 961 CGGCGGAAAC G'-CCGTGGCCG AGAGGAGCCT GCACAGGTGG TGCATGGCTG TCGTCAGCTC 1021 GTGTCGTGAG ATGTTGGGTT AAGTCCCGCA ACGAGCGCAA CCCCTGTCGT ATGTTGCCAT 1081 CATTCAGTTG GGGACTCGTA CGAGACTGCC GGCGTCAAGC CGGAGGAAGG TGGGGACGAC 1141 GTCAAGTCAT CATGCCCTTT ATGCCCTGGG CTGCACACGT GCTACAATGG CCGGTACAAC 1201 GGGCTGCGAG CCAGCGATGG CGAGCGAATC CCTCAAAACC GGTCCCAGTT CGGATCGGAG 1261 GCTGCAACCC GCCTCCGTGA AGTCGGAGTT GCTAGTAATC GCGGATCAGC ATGCCGCGGT 1321 GAATACGTTC CCGGGCCTTG TACACACCGC CCGTCACA ACCCGAGTTG TCTGCACCCG 1381 AAGTCGACGG CCCAACCCGC GAGGGGGGAG TCGCCGAAGG TGTGGGGAGT AAGGGGGGTG 1441 AAGTCGTAAC AAGGTAGCCG TACCGGAAGG TGCGGCT, sequence data of the microorganism compared to the gene sequences RNA of representative microorganisms that are part of the domain of the bacteria.

This comparative analysis showed the great correspondence with the bacteria of the genus Eubacteriales. However, it was not possible to find any gene sequence corresponding sufficiently to a known microorganism, which would result in the microorganism according to the invention being a microorganism within the genus Eubacteriales which has not been isolated and classified to date. . Physiological investigations, such as, for example, the spectrum of fermentation, the reduction of nitrate to nitrite, also clearly show the association with the genus Eubacteriales. Another additional objective of the present invention is to make available a method for obtaining a pure culture of the microorganism DSM 11798 and its mixed culture with the strain of Enterococo casselifiavus, DSM 11799, and other anaerobic microorganisms, in particular an optimization has been achieved in production both economically and quantitatively. To achieve this objective, the process according to the invention is carried out in such a way that a mixed culture DSM 11799 is obtained from the microorganism and the Enterococcus casselifiavus from the bovine rumen by culturing or fermenting at least twice in a series of dilutions and anaerobic culture conditions. In order to obtain the mixed culture and the pure culture of the microorganism according to the invention, it has been found favorable to cultivate and / or ferment in dilution series at least twice, since in this way an assured purity of the desired products, and, in particular, a removal of the by-products or interferences with unwanted microorganisms. In order to maintain the anaerobic conditions, the culture and / or the fermentation according to the invention was preferably carried out in a gas atmosphere of H2 and C02, the gas atmosphere has a H2: C02 ratio of 10:90 to 90: 10, in particular about 80:20, this being preferably selected in particular. For the growth of the microorganism according to the invention, anaerobic conditions with a low redox potential are important, it being surprisingly possible only to achieve a sufficiently rapid growth in the presence of H2. An even faster growth of the microorganism according to the invention can be achieved by carrying out the cultivation and / or fermentation at an overpressure of 0.2 to 3 bar, in particular of 0.5 to 1 bar, since this corresponds to a form of additional embodiment preferably. It is possible to achieve further improved growth of the DSM microorganism 11798 according to the invention by preferably carrying out the cultivation and / or fermentation at a temperature of 35 to 42 ° C, in particular about 37 ° C. The optimum pH for the culture or fermentation in the process according to the invention was preferably at a pH between 6 and 8 and in particular between 7 and 7.5. Under these conditions, it is possible to obtain a pure culture of the microorganism DSM 11798 and its mixed culture (DSM 11799) described above, in as short a time as possible and using relatively few dilution series. Optimum results can be achieved with the process according to the invention by preferably carrying out the cultivation and / or fermentation in a preparation medium comprising components selected from: arginine, citrulline, peptone, yeast extract, mixture (s) of fatty acid, mineral solution (s), glucose, hemin solution, menadione, vitamin solution, traces of elements and reducing agents. The components contained in the preparation of the medium are in this case particularly interchangeable, it being possible, for example, through the addition of glucose to achieve a balance shift in the mixed culture in the direction of Enterococcus casselifiavus or corresponding to other anaerobic microorganisms, it being possible to control the process specifically depending on the amount of glucose added. According to the particularly preferred aspect of the invention, at the start of cultivation and / or fermentation, 0.1 to 0.5% by weight, in particular 0.2% by weight, of glucose is added. By adding 0.1 to 0.5% by weight of glucose, the growth of Enterococcus casselifiavus promotes the initiation of cultivation and / or fermentation, which leads to a fall in redox potential. By decreasing the redox potential, the optimum growth conditions for the microorganism according to the invention were created, such that, for example, chemicals, such as cysteine, can be dispensed by the addition in the preparation of the medium. controlled glucose. In order to achieve the detoxification of the mycotoxins, in particular trichothecenes, towards another advantageous effect with the microorganism and / or the mixed cultures according to the invention, the enzyme preparation of the active microorganism, trichothecene-detoxifying and / or other Anaerobic organisms can preferably also be added according to the invention. To obtain a pure culture of the microorganism DSM 11798, the process according to the invention is preferably carried out in such a way that the pure culture of the microorganism DMS 11798 is obtained from the DSM 11799 culture or fermentation by at least two series of further dilutions in the preparation of the medium, in particular with the addition of L-arginine as a growth stimulator. By carrying out two additional dilution series in the preparation of the culture solution or fermentation medium, the microorganism DMS 11798 can be obtained completely pure from the mixed culture with Enterococcus casselifiavus, the addition of the growth stimulator L-arginine advantageously changes the equilibrium in the direction of the pure culture of the microorganism. In this connection, the growth of the bacterium according to the invention is promoted the higher the concentration of L-arginine. In order to lower the redox potential of the preparation of the medium additionally, a process according to the invention is preferably used, in which, for the fermentation of the pure culture of the preparation of the medium, from 1 to 4% was added by weight of the reducing agent, in particular of the mixture of cysteine / sodium sulfide / sodium carbonate solution. ' Particularly, for reasons of economy, according to the invention, the addition of the reducing agent is kept as low as possible, demonstrating in the course of the comparison experiments that an increase in the concentration of the reducing agent beyond 4% by weight does not cause an additional acceleration- of the growth of the microorganism. In order to obtain a storable finished product, the process according to the invention is preferably continued by conditioning the culture or fermentation solution by concentrating, in particular by centrifuging or filtering and / or stabilizing, in particular by freezing or drying by spray or encapsulate. In this connection, for example, the culture or fermentation solution is concentrated in a first step by removing the liquid by centrifugation or filtration and / or by carrying out the stabilization directly from the fermentation solution, preferably with the addition of a filler. or carrier material, such as aluminum silicates, kieselguhrs, carbohydrates, sugar alcohols, starches, milk and whey powder, protein hydrolysates, yeasts and PVPP. By adding these carriers or fillers, it is possible to obtain in the next stabilization step, in particular, in the freeze-drying, spray-drying, encapsulation of the pelletizing step, a solid product in which the pure culture of the microorganism DSM 11798 or their mixed culture with the Enterococcus casselifiavus strain, DSM 11799, or other anaerobic microorganisms, in particular of the genus Enterococcus, Streptococcus, Lactococcus, Bacillus, Lactobacillus, which are suitable for detoxification of trichothecenes, are deposited directly on a carrier, as a result from which a product particularly easy to handle and store itself is obtained as metabolically favorable. By depositing the microorganism or its mixed culture in a substance having a large internal surface area, such as clayey soils, aluminum silicates, zeolites, and the like, the intentional degradation of the trichothecenes according to the invention is further facilitated, since these are physically linked to the substance having a large internal surface area, by which the biochemical attack with the microorganism according to the invention is distinctly facilitated. According to the invention, the microorganism is additionally used in a pure and / or mixed culture (DSM 11798, DSM 11799) for the production of a food additive. A particularly preferred use according to the invention essentially results because the pure and / or mixed culture (DSM 11798 and DSM 11799) is employed as a freeze-dried or spray-dried and / or encapsulated or pelleted immobilized, if correct with the addition of a carrier material. The pure culture and / or the mixed culture of the microorganism according to the invention and the spray-dried immobilization can be used directly as an additive for food, it being possible further to mix the additive for food to the food directly during the preparation and / or to mix it inside. of the food either in solid or liquid form during feeding of the animals. In order to achieve as complete a chemical degradation or conversion as possible of the trichothecenes within physiologically acceptable substances, an additive according to the invention for the inactivation of trichothecenes in food or in the digestive tract of animals is essentially characterized in that the food additive contains a pure and / or mixed culture of a microorganism according to the invention or a microorganism prepared according to the invention in an amount of the order of 105 to 1012 cells / kg, in particular of the order of 107 to 109 cells / kg, per 1000 kg of feed. By using 105 to 1012 cells / kg, in particular 107 to 109 cells / kg, of the microorganism according to the invention or of the mixed culture according to the invention of the microorganism and Enterococcus casselifiavus and other anaerobic organisms, in particular of the genus of Enterococcus, Streptococcus, Lactococcus, Bacillus, Lactobacillus, which are suitable for the detoxification of trichothecenes, it is possible to convert high concentrations of trichothecenes, in particular of deoxynivalenol, T-2 toxin, HT-2 toxin, nicalenol, monoacetoxiscirpenol, diacetoxyscirpenol , tricodermol, verrucarin, rorodine, acetyldeoxy-nivale-nol, isotrichdermin, hydroxyisotrichdermin, calonectrin, tetraol T-2, triol T-2, deacetyl-neosolaniol, neosolaniol, acetylneosolaniol, esporo-triquiol, trichothriol, sambucinol and culmorin in foods within chemically non-harmful substances, such as detoxinivalenol (DOM-1) deep-toxic metabolite, so that, when using the additive for food according to the invention, an increase in the productivity of the animals can be achieved, and, in accounting for reduced toxicity, an improved rate of feed conversion. In order to further facilitate the biochemical degradation of the mycotoxins, in particular of the trichothecenes, according to the invention a carrier and / or filler material can preferably be additionally contained in the food additive in an amount of 0. 5 to 8 kg / 1000 kg, in particular from 0.7 to 4 kg / 1000 kg, of the feed. By means of the addition of the carrier and / or filler materials, it is possible, if desired, to bind the mycotoxins and also other harmful sances to be degraded, which can be contained in the food, physically to the sances, as a result of which are no longer available for metabolization. In this case, in particular, aluminum silicates, kieselguhrs, carbohydrates, sugar alcohols, starch, milk and whey powders, protein hydrolysates, yeasts, and / or PVPP are used as a carrier and / or filler material, these carrier and / or filler materials have proven to be particularly advantageous for binding toxins, in particular tricotoxins. A particularly preferred food additive is characterized in that the feed additive consists of a mixture of 1 to 65% by weight, in particular 5 to 50% by weight, of immobilized spray-dried or freeze-dried micro-organisms and 99 to 35 % by weight, in particular 95 to 50% by weight, of the carrier and / or filler material. Food additives of this type are suitable, in particular, for the inactivation of deoxynivalenol (DON), T-2 toxin, HT-2 toxin, nivalenol, monoacetoxiscirpenol, diacetoxyscirpenol, trichodermol, verrucarin, rorodine, acetylodeoxynivalenol, isotrichdermin, hydroxyisotrichdermin, calonectrin, tetraol T-2, triol T-2, deacetyl-neosolaniol, neosolaniol, acetylneosolaniol, esporo-triquiol, tricotriol, sambucinol and culmorina in the food and in the digestive tract of animals. The invention is further explained below by means of the characterization of the microorganism according to the invention, its growth and activity conditions and the formation of metabolic products of trichothecenes with the aid of the microorganism according to the invention and by means of examples of conditioning of feeding tests. The microorganism according to the invention is a tricothecene transforming active strain, in particular a deoxynivalenol transforming strain, and is obtained from bovine rumen through repeated culture in an optimized nutrient medium under anaerobic culture conditions, namely a gas atmosphere of C02: H2 (20/80 v / v) and an overpressure of 0.5 to 1.5 bar, means PYG and PY were used here as a medium preparation, which in each case consisted of concentrations that differed in two mineral solutions different, menadione stock solution, hemin solution, yeast extract, peptone, glucose that were additionally added to the PYG medium. In order to lower the redox potential, 2 to 4% by weight of a reducing solution consisting of a solution of cysteine / Na 2 S / Na 2 CO 3 was added to the medium PYG and to the medium PY and the pH was adjusted to a value of 7. to 7.5 by gassed C02. It is possible that with the help of this medium preparation a pure culture of the microorganism DSM 11798 is obtained by carrying out several series of dilutions, and also, in particular with the medium PYG, a mixed culture of the microorganism and the Enterococcus casselifiavus is obtained. (DSM 11799). The growth of the microorganism is achieved exclusively under strictly anaerobic conditions with a sufficiently low redox potential and the presence of H2. The optimum growth of the microorganism can be reached at approximately 37 ° C, it being also possible, however, to achieve an adequate growth of the microorganism between 35 ° C and 38 ° C. To cultivate the pure culture of the microorganism DSM 11798, the L-arginine in liquid medium has a stimulating effect.

When using the microorganism according to the invention, it is possible, in trichothecenes, to detoxify these through a reductive division of the toxic epoxide ring 12,13. Squently the reaction scheme is shown here with the aid of the trichothecene generally and also the specific tricothecene deoxynivalenol.

Tpcoteceno reduced form non-toxic Deoxynivalenol DOM-1 Subsequently, the fermentation conditions and the fermentation processes which can be used as an example form for the fermentation of the microorganism DSM 11798 according to the invention and in cocultivation with other facultative and anaerobic microorganisms and in the mixed culture with Enterococo casselifiavus (DSM 11799) will be illustrated. . Fermentation Conditions: The fermentation temperature between 35 and 42 ° C, in particular about 37 ° C; The pH range for fermentation between 6 and 8, in particular from 7.0 - 7.5; The redox potential: 0 - -350 mV, depending on the way in which the procedure is carried out; Gas atmosphere: H2 / C02 10:90 to 90:10, in particular 80 Fermentation repression: 02 - 3 bar, in particular 0.5 to 1 bar. Essential constituents of the medium: arginine, citrulline, yeast extract, peptones, hemin, and substances containing hemin, low fatty acids, mineral solution, carbonate buffer (sodium carbonate + C02), optionally glucose, trace element solution, Vitamin solution and reducing agent. Here you can select several ways to carry out the fermentation process: 1) Batch fermentation of the pure culture DSM 11798: Procedure: sterilization of the medium at 121 ° C and 1. 5 bar or sterile filtration. Cooling of the medium to the fermentation temperature of 35-42 ° C, in particular 37 ° C, while gasifying with sterilized C02 and adding sodium carbonate and reducing agent. The gasification continues until obtaining a pH of 6-8, in particular of 7 - 7.5. Subsequently 1-10% inoculum that has been previously cultured for 24-48 hours, in particular 5%, is added. Fermentation is carried out until the beginning of the stationary phase - the duration is approximately 20 - 50 hours, depending on the concentration of the substrate or until the microorganism count is reached in the form of the range of 1013 - 1016. The procedure is controls essentially by the concentration of the substrate. 2) Fermentation by feed batch of the pure culture DSM 11798: Procedure: sterilization, buffering, reduction and inoculation of the medium as in number 1. The production of the biomass is increased by means of batch or by continuous addition of the substrate, for example , arginine, citrulline. The culture is maintained in the exponential phase of growth by maintaining the concentration of the substrate at a relatively high level. A fermentation time of up to 60 hours is possible when using this method to carry out the procedure. The process is controlled by the addition of the substrate and the fermentation time (accumulation of the final metabolic products). 3) Continuous fermentation of the pure culture DSM 11798: Procedure: sterilization, buffering, reduction and inoculation of the medium as in number 1. Fermentation by batch until the start of the stationary phase, after conversion of the continuous fermentation by means of the addition of the sterile nutrient solution. The effluent is collected in a storage tank and is worked by batch or by spray drying continuously. 4) Fermentation of the pure culture DSM 11798 in coculture with another facultative and strictly anaerobic microorganism or the fermentation of the coculture DSM 11799. Examples of cocultures that can be used: Producers of H2 DSM 11798 + Butirvibrio sp. DSM 11798 + Ru inococo sp. Probiotics DSM 11798 + Enterococcus casselifiavus = DSM 11799 DSM 11798 + Streptococcus sp. (enterococci, lactic acid streptococci, anaerobic streptococci) DSM 11798 + Leuconostoc sp. DSM 11798 + Pediococcus sp. DSM 11798 + Lactobacillus sp.

DSM 11798 + Bifidobacteria sp DSM 11798 + Bacillus sp. DSM 11798 + Mega spherical sp. Yeasts DSM 11798 + Sacaromices sp. DSM 11798 + Klivero ices sp. DSM 11798 + Cándida sp. The use of coorganism in fermentation serves, on the one hand to reduce the redox potential in the fermentation, to produce hydrogen for DSM 11798 and as a protective organism in the preparation and in the stabilization. A minimization of the losses of counting of the microorganism is carried out here and these 'serve in some cases as additional promoters of productivity in animal production. 4a) Fermentation by batch in coculture I) The coorganism is previously cultured in a medium containing carbohydrates. The medium described above, which, however, does not contain a reducing agent, but additionally contains carbohydrates for this purpose, which are used to reduce the redox potential. Subsequent inactivation of the co-organism and inoculation of the DSM 11798. II) The simultaneous inoculation of the coorganism and DSM 11798 and the addition of 0.1 1% carbohydrate to the medium. The medium described above is used, which, however, does not contain any reducing agent but additionally contains carbohydrates for this purpose. The growth of the coorganism is promoted - a rapid fall in the redox potential - "the DSM 11798 begins to grow on account of the ideal growth conditions III) In combination with I + II: at the end of the fermentation the carbohydrates are added for the reference of the coorganism, this leads to a protective effect (oxygen) in the conditioning and stabilization on the account of the increase in the production of the biomass 4b) Fermentation by batch of food in coculture: *) phase by lot corresponding to 4al, subsequent continuous addition / per batch of substrate (arginine, citrulline) corresponding to 2. **) batch phase corresponding to 4al, subsequently continuous / batch addition of a substrate combination (arginine / carbohydrates or citrulline / carbohydrates). 4c) Continuous fermentation of the cocultivation: The phase by lot corresponding to 4al, subsequent conversion to continuous fermentation by means of the addition of a solution nutrient ion containing arginine / carbohydrate- or citrulline / carbohydrate-. Condition as in number 3.

Conditioning of fermentation products: 1) Concentration by membrane filtration method (ultrafiltration, microfiltration) or centrifugation. Drying by "spray or subsequent lyophilization with or without organic and / or inorganic carrier materials 2) Direct spray drying or freeze drying with or without organic and / or inorganic carrier materials 3) Continuous spray drying of the fermentation broth with or without organic and / or inorganic carrier materials 4) Encapsulation or pelletization in combination with 1, 2 or 3. To review this activity of the DON biotransformer strain (DSM ^ 11798) in the intestinal medium, an in vi tro model was developed using the In this connection, in a first experiment, an in vitro model was developed with the intestinal contents in tampon with the addition of a lyophilisate.The small intestine and the large intestine of a recently killed pig were kept under an atmosphere of C02 The emptying of the contents of the anterior, middle and posterior sections of the small intestine and large intestine in individual vessels was carried out. s under an atmosphere of C02. ml of anaerobic buffer + 1 g of intestinal content (gassed C02) + DON at 37 ° C were incubated under an atmosphere of C02 or H2 / C02. It was found "that in the anterior section of the small intestine there is a marked positive effect on the addition of the active lyophilisate." The fact is particularly significant here, that the marked activity can also be detected under pure gassed C02. Vi tro using complete pieces of pig intestine with the addition of an active suspension The pieces of pig intestine (anterior, middle, posterior, large intestine, each 6-8 cm) were incubated for 24 hours with 200 ppm DON in an anaerobic buffer, reduced, preincubated (30 ml) at 37 ° C. This variant of the modified in vitro test has the advantage that the physiological condition of the intestine is poorly affected, since immediately after killing the whole intestine. It is transported to the laboratory and the pieces of the desired length are tied there.Then, the pieces are separated and incubated in a buffering solution with DON and culture active.The results are clear. Deepoxidation of DON to DOM-1 can be achieved by active culture. It is significant that this activity can be demonstrated in all sections of the intestine, with the highest activity being that which must be found particularly in the bowel. This is important so far since most of the absorption of the food and thus also the release of mycotoxins is also carried out there. The action of the microorganism according to the invention, both in pure culture (DSM 11798) and in mixed culture of the microorganism and Enterococcus casselifiavus (DSM 11799) as well as other anaerobic microorganisms, in particular of the genus Enterococcus, Streptococcus, Lactococcus, Bacillus o Lactobacilli, will subsequently be demonstrated with the help of a laboratory protocol concerning chicken cell cultures and in examples of feeding pigs and chickens. With the help of a laboratory protocol using chicken cell cultures, it has been demonstrated for the microorganism that it is able to chemically degrade the mycotoxins, in particular trichothecenes, and especially deoxynivalenol and T-2 toxin, in particular to reduce them and convert them into physiologically acceptable substances, in the case of deoxynivalenol in the deepoxymetabolite thereof, namely DOM-1. To culture chicken lymphocytes, the following conditions were followed: Number of cells used: 2 x 106 cells / ml Stimulation: ConA 5μg / ml Mycotoxins: DON, DOM-1 and T2 toxin Concentration Range: "DON: 10-0.08 μg / ml DOM-1: 232-1.81 μg / ml Toxin T2: 30-0.234 ng / ml The total incubation time: 44 hours, of which 16 hours were labeled during the culture in an incubator: 40 ° C, 5% C02, saturated steam water atmosphere. With the help of a laboratory protocol using cultures of chicken lymphocyte cells, it was shown for the active culture that it has the ability to biochemically degrade mycotoxins, in particular trichothecenes, particularly to reduce them, and to convert them into physiologically acceptable substances. This is shown in the following example form of DON and its DOM-1 deepoxymetabolite: Microscopic review of the cell culture: The culture of the cell containing the chicken lymphocytes was examined microscopically continuously during the culture. Revision after 20 hours: The unstimulated cells were distributed thickly and uniformly, the revisions with ConA showed powerful stimulation and pronounced proliferation of foci. DON: the proliferation of the foci was observed in all the stages of concentration, this being outstanding, however, "in a concentration of the range of 10 μg / ml-0.625 μg / ml marked reductions in the proliferation of foci were observed with a increase in toxin concentration DOM: proliferation of foci in all stages of concentration without an apparent change compared to the control of up to a higher concentration stage of 58 μg / ml This means that even after 20 hours found an activity of the cell in the toxin lot from a concentration of 0.625 μg / ml, whereas with the deepoxymetabolite no negative effect on the cell culture was observed at a concentration of 58 μg / ml. after 28 and after 44 hours: The control lots (unstimulated and ConA) remain unchanged.The action of the DON in the culture of the cell has been increased further. In series in which a change was found even after 20 hours, marked damage to the cells was observed. Even after these incubation times, it was not possible to find any adverse effect on the activity of the cell even at a concentration of the deepoxymetabolite of 58 μg / ml. In a second experiment, the action of an additive for food according to the invention was investigated in a feed comprising the deoxynivalenol mycotoxin in an amount of 450 ppb that was fed to breeding piglets. Animals: "Large White" and "Land-rasse" weaned piglets were divided into negative comparison groups, positive comparison groups and test groups. The experiment started directly after weaning (age of the piglets, 20 to 22 days); the productivity parameters of the animals were determined 14 days later. Food: They were fed a piglet starter feed commercially available to pigs 7 days after weaning, after which they received a commercially available feed for piglet growth. The deoxynivalenol mycotoxin was mixed with a small aliquot of the food in a concentration of 450 ppb (dissolved in ethanol) in order to introduce it into the food of the positive comparison group and the test group. The food was provided ad libi tum. Food additive dose: A food additive was added to the feed of the test group at a dose of 1 kg / t. The food additive used was mixed with a culture of the microorganism according to the invention, with Enterococcus casselifiavus (DSM 11799), aluminum silicate was added to the mixed culture as a carrier. Microorganism count: 4 x 108 cells / kg of finished feed Results: The results are summarized in Table 1, which shows the growth in average weight and the average feed conversion rate in this test, was fed to the negative comparison group with food that is not composed of the mycotoxin nor by the microorganism according to the invention, the positive comparison group was fed with feed composed only of the mycotoxin, and the test group was fed with compound feed with the mycotoxin and the microorganism according to the invention. Table 1 Discussion: It is evident from this test that the food additive has the ability to compensate for the adverse effect of deoxynivalenol contamination on weaned piglets, and that the piglets that received both the mycotoxin and the food additive consumed essentially in the same food quantities that in the negative comparison group and also showed an identical feed conversion rate (FCR). These results made it clear that it was almost entirely possible to compensate for the negative effect of deoxynivalenol through the mixed culture according to the invention (DSM 11799). In a subsequent test, the effect of the food additive according to the invention against contamination with trichothecenes in chicken feed was shown. The parameters used were final weight, food intake and feed conversion rate as well as chick losses. Clinical symptoms were also recorded. Animals: Cobb chickens were investigated from the first day of life onwards. The test was conducted using three groups, composed of 10,700, 10,900 and 15,700 chickens. A specific chicken feed ad libi tum a was administered. The chicken. Feed Additive Dosage: The food additive was contained in a dose of 1 kg / t of chicken feed, only the test group received the feed additive. The food additive used was a pure culture of the microorganism (DSM 11798). Microorganism Count: 1 x 109 cells / kg of finished feed Trichothecenes were fed to the test group and to the positive comparison group in a total amount of 750 ppb (500 ppb of DON and 250 ppb of T-2 toxin). ). Results: The following table shows the productivity parameters of the three groups. Table 2 Clinical observations Clear oral irritation was evident in many of the animals in the positive comparison group. Discussion: Even in the present case, the food additive had the ability to completely compensate for the adverse effect of trichothecene in birds in relation to productivity parameters and clinical symptoms. Even in the case of the birds, it is observed that the chickens of the test group, which also received the microorganism DSM 11798 in addition to the mycotoxins, still had a higher average final weight than the negative comparison group, and this with an intake of slightly lower average food, as a result of this, somehow the productivity parameters were still improved in relation to the negative comparison group. By using the food additive according to the invention, which comprises the microorganism according to the invention, it shows that not only was the adverse effect of the mycotoxins compensated, but it was also possible to achieve a further increase in the productivity in the animals who received the microorganism DSM 11798.

CLAIMS (amended) 1. A microorganism of the genus Eubacteriales, DSM 11798, in pure culture, in mixed culture with the strain d Enterococo casselifiavus, DSM 11799, or in mixed culture with other anaerobic microorganisms for the detoxification of trichothecenes by reductive division of the ring epoxy -12.1 thereof. 2. The microorganism according to claim 1, characterized in that the anaerobic microorganisms are selected from the genus Enterococcus, Streptococcus, Lactococcus, Bacillus, or Lactobacillus. 3. A microorganism according to claim 1 or 2, characterized in that it detoxifies deoxynivalenol (DON), T-2 toxin, HT-2 toxin, nivalenol, monoacetoxiscirpenol, diacetoxyscirpenol, trichodermol, verrucarin, rorodine, acetylodeoxynivalenol, isotrichidolamine, hydroxyisotrichidolamine, calonectrin, tetraol T-2, triol T-2, deacetyl-neosolaniol, neosolaniol, acetylneosolaniol, esporo-triquiol, tricotriol, sambucinol and culmorina. 4. A microorganism according to any of Claims 1, 2 or 3, characterized in that the microorganism detoxifies the trichothecenes through a reductive biotransformation of the epoxide group that is contained in the molecule.

. A microorganism according to one of Claims 1 to 4, characterized in that it is a gram-positive, rod-shaped, anaerobic bacteri that does not form spores which occurs individually, in pairs or in long chains. 6. The microorganism according to one of Claims 1 to 5, characterized Porqu contains an RNA sequence, namely 1 CCTGGCTCAG GATGAACGCT GGCGGCGTGC TTAACACAT CAAGTCGAAC GGATAACCCG 61 CCTCCGGGCG GTTATAGAGT GGCGAACGGG TGAGTAACAC GT.GACCAACC TACCTCCCAC 121 TCCGGGATAA CCCAGGGAAA CCTGCGCTAA TACCGGATAC TCCGGGGCCC CCGCATGGGG 181 GCGCCGGGAA AGCCCCGACG GTGGGAGATG GGGTCGCGGC CTATTAGGTA GTCGGCGGGG 241 TAACGGCCCA CCGAGCCCGC GATAGGTAGC CGGGTTGAGA GACCGATCGG CCACATTGGG 301 ACTGAGATAC GGCCCAGACT CCTACGGGAG GCAGCAGTGG GGAATTTTGC GCAATGGGGG 361 AAACCCTGAC GCAGCAACGC CGCGTGCGGG ACGAAGGCCT TCGGGTTGTA AACCGCTTTC 421 TACCTGCAGA AGCAGGGAAG AAGTTGACGG AGAAGCTCCG GCTAACTACG TGCCAGCAGC 481 CGCGGTAATA CGTAGGGAGC GAGCGTTATC CGGATTTATT GGGCGTAAAG CGCGCGTAGG 541 CGGGCGCTTA AGCGGAATCT CTAATCTGAG GGCTCAACC CCAGCCGGAT TCCGAACTGG 601 GCGCCTCGAG TTCGGTAGAG GAAGACGGAA TTCCCAGTG AGCGGTGAAA TGCGCAGATA 661 TTGGGAAGAA CACCGATGGC GAAGGCAGTC TTCTGGGCC TAACTGACGC TGAGGTGCGA 721 AAGCTAGGGG AGCGAACAGG ATTAGATACC CTGGTAGTC TAGCCGTAAA CGATGGGCAC 781 TAGGTGTGGG GGGGAATGCC CCTCCGTGCC GCAGCTAAC CATTAAGTGC CCCGCCTGGG 841 GAGTACGGCC GCAAGGCTAA AACTCAAAGG AATTGACCGG GGCCCGCACA AGCAGCGGAG 901 CATGTGGCTT AATTCGAAGC AACGCGAAGA ACCTTACCAG GGCTTGACAT GCAGGTGAAG 961 CGGCGGAAAC GCCGTGGCCG AGAGGAGCCT GCACAGGTGG TGCATGGCTG TCGTCAGCTC 1021 GTGTCGTGAG ATGTTGGGTT AAGTCCCGCA ACGAGCGCAA CCCC? GTCGT ATGTTGCCAT 1081 CATTCAGTTG GGGACTCGTA CGAGACTGCC GGCGTCAAGC CGGAGGAAGG TGGGGACGAC 1141 GTCAAGTCAT CATGCCCTTT ATGCCCTGGG CTGCACACGT GCTACAATGG CCGGTACAAC 1201 GGGCTGCGAG CCAGCGATGG CGAGCGAATC CCTCAAAACC GGTCCCAGTT CGGATCGGAG 1261 GCTGCAACCC GCCTCCGTGA AGTCGGAGTT GCTAGTAAT GCGGATCAGC ATGCCGCGGT 1321 GAATACGTTC CCGGGCCTTG TACACACCGC CCGTCAC ACCCGAGTTG TCTGCACCCG 1381 AAGTCGACGG CCCAACCCGC GAGGGGGGAG TCGCCGAAGG TGTGGGGAGT AAGGGGGGTG 1441 AAGTCGTAAC AAGGTAGCCG TACCGGAAGG TGCGGCT, 7. A method for obtaining a culture of the microorganism according to one of Claims 1 to 6, characterized in that a mixed culture DSM 11799 is obtained from the microorganism and Enterococcus casselifiavus from the bovine rumen. when cultivating and / or fermenting for at least twice under conditions of dilution series and anaerobic culture. 8. The procedure according to the Claim 7, characterized in that the cultivation and / or fermentation is carried out in a gas atmosphere of H2 and C02. 9. The process according to claim 8, characterized in that the gas atmosphere is selected with a H2: C02 ratio of 10:90 to 90:10, in particular of about 80:20. The process according to one of Claims 7 to 9, characterized in that the cultivation and / or fermentation is carried out at an overpressure of 0.2 to 3 bar, in particular 05. to 1 bar. The process according to one of Claims 7 to 10, characterized in that the cultivation or fermentation is carried out at a temperature of 35 to 42 ° C, in particular at 37 ° C. The process according to one of Claims 7 to 11, characterized in that the cultivation and / or fermentation is carried out with a pH of the order of 6 and 8, in particular with a pH of the order of 7 and 7.5. 13. A procedure according to one of the Claims 7 to 12, characterized in that the cultivation and / or fermentation is carried out in a preparation of a medium comprising components selected from: arginine, citrulline, peptone, yeast extract, acid mixture (s) (s) fat (s), mineral solution (s), glucose, hemin solution, menadione, vitamin solution, trace elements, reducing agents. The method according to one of Claims 7 to 13, characterized in that 0.1 to 0.5% by weight, in particular 0.2% by weight, glucose is added at the start of the culture and / or fermentation. 15. The process according to one of claims 7 to 14, characterized in that enzyme preparations of the tricothecene detoxifying active microorganism and / or other anaerobic microorganism are added. The process according to one of Claims 7 to 15, characterized in that the pure culture of the microorganism DSM 11798 is obtained from the culture and / or fermentation solution through at least two additional dilution series in the preparation of the medium, in particular by adding L arginine as a growth stimulator. 17. The procedure according to the Claim 16, characterized in that, for the fermentation of the pure culture of the preparation of the medium, 1 to 4% by weight of a reducing agent is added, in particular a mixture of cysteine / sodium sulfide / sodium carbonate in solution. 18. A procedure according to one of the Claims 7 to 17, characterized in that the solution in culture and / or fermentation is conditioned by concentrating, in particular by centrifuging or filtering and / or stabilizing, in particular, by freeze drying or spray drying or encapsulation. 19. The process according to claim 18, characterized in that the stabilization is carried out with the addition of a filler or carrier material, such as aluminum silicates, kieselguhrs, carbohydrates, protein hydrolysates, yeasts, PVPP.

. The use of the microorganism in pure and / or mixed culture (DSM 11798, DSM 11799) for the preparation d μn additive for food. 21. The use according to claim 20, characterized in that the pure and / or mixed culture (DSM 11798 and DSM 11799) is used as a freeze drying or a spray drying and / or encapsulated immobilization, potentially when added to the carrier material. . 22. The additive for food for the inactivation of trichothecenes in food or in the digestive tract of animals, characterized in that the food additive contains a pure and / or mixed culture of a microorganism according to one of Claims 1 to 6 or a microorganism prepared according to one of Claims 7 to 19, in an amount of 105 1012 cells / kg, in particular 107 to 109 cells / kg, per 1000 kg of feed. 23. The food additive according to claim 22, characterized in that it additionally contains a carrier and / or filler material in an amount of the order of 0.5 to 8 kg / 1000 kg, in particular 0.7 to 4 kg / 1000 kg, of the food. The food additive according to claim 23, characterized in that aluminum silicates, kieselguhrs, carbohydrates, sugar alcohols, starch, milk and whey powder, protein hydrolysates, yeasts and / or PVPP are contained as carrier material or filling. The food additive according to any of Claims 22, 23, or 24, characterized in that the feed additive consists of a mixture of 1 to 65% by weight, in particular 5 to 50% by weight, of immobilized spray drying or freeze drying of the microorganism and 99 to 35% by weight, in particular 95 to 50% by weight, of carrier and / or filler material. 26. The use of a feed additive according to any of claims 22 to 25 for the inactivation of deoxynivalenol (DON), T-2 toxin, HT-2 toxin, nivalenol, monoacetoxiscirpenol, diacetoxyscirpenol, trichodermol, verrucarin, rorodine, acetyldeoxynivalenol, isotriodermin, hydroxyisotrichdermin, calonectrin, tetraol T-2, triol T-2, deacetylneosolaniol, neosolaniol, acetylneosolaniol, sporotriquiol, trichotriol, sambucinol and culmorin in a food or in the digestive tract of animals.

SUMMARY OF THE INVENTION A microorganism of the genus Eubacteriales, and its obtaining and use, which are suitable in pure culture DSM 11798, and / or in mixed culture with a strain of Enterococcus casselifiavus, DSM 11799, or in mixed culture with other anaerobic microorganisms for the detoxification of trichothecenes. An additive for food for the inactivation of trichothecenes in food or in the digestive tract of animals containing a pure and / or mixed culture of a microorganism (DSM 11798 or DSM 11799) or a mixed culture with other anaerobic microorganisms in an amount of the order from 0.2 to 3 kg, in particular from 0.5 to 2.5 kg per 1000 kg of feed.

Claims (26)

1. CLAIMS (Original) 1. A microorganism of the genus Eubacteriales, DSM 11798, which in pure culture, in mixed culture with the strain Enterococo casselifiavus, DSM 11799, or in culture mixtures with other anaerobic microorganisms is suitable for the detoxification of trichothecenes.
2. 2. The microorganism according to claim 1, characterized in that the anaerobic microorganisms are selected from the genus Enterococcus, Streptococcus, Lactococcus, Bacillus, or Lactobacillus.
3. 3. A microorganism according to any one of Claims 1 or 2, characterized in that it is suitable for the detoxification of deoxynivalenol (DON), T-2 toxin, HT-2 toxin, nivalenol, monoacetoxiscirpenol, diacetoxyscirpenol, trichodermol, verrucarin, rorodine, acetyldeoxy-nivalenol, isotriodermin, hydroxyisotrichdermin, calonectrin, tetraol T-2, triol T-2, deacetyl-neosolaniol, neosolaniol, acetylneosolaniol, sporotriquiol, trichotriol, sambucinol and culmorin.
4. 4. A microorganism according to any of Claims 1, 2 or 3, characterized in that the microorganism detoxifies the trichothecenes through reductive biotransformation of the epoxide group that is contained in the molecule.
5. 5. The microorganism according to one of claims 1 to 4, characterized in that it is a gram-positive, rod-shaped, anaerobic bacterium that does not form spores which occurs individually, in pairs or in long chains.
6. 6. The microorganism according to one of the Claims 1 to 5, characterized in that it contains a 16S RNA sequence, namely: 1 CCTGGCTCAG GATGAACGCT GGCGGCGTGC TTAACACATG CAAGTCGAAC GGATAACCCG 61 CCTCCGGGCG GTTATAGAGT GGCGAACGGG TGAGTAACAC GTGACCAACC TACCTCCCAC 121 TCCGGGATAA CCCAGGGAAA CCTGCGCTAA TACCGGATAC TCCGGGGCCC CCGCATGGGG 181 GCGCCGGGAA AGCCCCGACG GTGGGAGATG GGGTCGCGGC _ CTATTAGGTA GTCGGCGGGG 241 TAACGGCCCA CCGAGCCCGC GATAGGTAGC CGGGTTGAGA GACCGATCGG CCACATTGGG 301 ACTGAGATAC GGCCCAGACT CCTACGGGAG GCAGCAGTGG GGAATTTTGC GCAATGGGGG 361 AAACCCTGAC GCAGCAACGC CGCGTGCGGG ACGAAGGCCT TCGGGTTGTA AACCGCTTTC 421 AGCAGGGAAG AAGTTGACGG TACCTGCAGA AGAAGCTCCG GCTAACTACG TGCCAGCAGC 481 CGCGGTAATA CGTAGGGAGC GAGCGTTATC CGGATTTATT GGGCGTAAAG CGCGCGTAGG 541 CGGGCGCTTA AGCGGAATCT CTAATCTGAG GGCTCAACC CCAGCCGGAT TCCGAACTGG 601 GCGCCTCGAG TTCGGTAGAG GAAGACGGAA TTCCCAGTG AGCGGTGAAA TGCGCAGATA 661 TTGGGAAGAA CACCGATGGC GAAGGCAGTC TTCTGGGCC TAACTGACGC TGAGGTGCGA 721 AAGCTAGGGG AGCGAACAGG ATTAGATACC CTGGTAGTCC TAGCCGTAAA CGATGGGCAC 781 TAGGTGTGGG GGGGAATGCC CCTCCGTGCC GCAGCTAACG CATTAAGTGC CCCGCCTGGG 841 GAGTACGGCC GCAAGGCTAA AACTCAAAGG AATTGACCGG GGCCCGCACA AGCAGCGGAG 901 CATGTGGCTT AATTCGAAGC AACGCGAAGA ACCTTACCAG GGCTTGACAT GCAGGTGAAG 961 CGGCGGAAAC GCCGTGGCCG AGAGGAGCCT GCACAGGTGG TGCATGGCTG TCGTCAGCTC 1021 GTGTCGTGAG ATGTTGGGTT AAGTCCCGCA ACGAGCGCAA CCCCTGTCGT ATGTTGCCAT 1081 CATTCAGTTG GGGACTCGTA CGAGACTGCC GGCGTCAAGC CGGAGGAAGG TGGGGACGAC 1141 GTCAAGTCAT CATGCCCTTT ATGCCCTGGG CTGCACACGT GCTACAATGG CCGGTACAAC 1201 GGGCTGCGAG CCAGCGATGG CGAGCGAATC CCTCAAAACC GGTCCCAGTT CGGATCGGAG 1261 GCTGCAACCC GCCTCCGTGA AGTCGGAGTT GCTAGTAATC GCGGATCAGC ATGCCGCGGT 1321 GAATACGTTC CCGGGCCTTG TACACACCGC CCGTCACA ACCCGAGTTG TCTGCACCCG 1381 AAGTCGACGG CCCAACCCGC GAGGGGGGAG TCGCCGAAGG TGTGGGGAGT AAGGGGGGTG 1441 AAGTCGTAAC AAGGTAGCCG TACCGGAAGG TGCGGCT,
7. 7. A method for obtaining a culture of the microorganism according to one of Claims 1 to 6, characterized in that a mixed culture DSM 11799 is obtained from the microorganism and the Enterococcus casselifiavus from the bovine rumen at cultivate and / or ferment at least twice under conditions of dilution series and anaerobic culture.
8. 8. The process according to claim 7, characterized in that the cultivation and / or the fermentation is carried out in a gas atmosphere of H2 and C02.
9. The method according to Claim 8, characterized in that the gas atmosphere is selected with. a H2: C02 ratio of 10:90 to 90:10, in particular of about 80:20.
10. The process according to one of Claims 7 to 9, characterized in that the cultivation and / or fermentation is carried out at an overpressure of 0.2 to 3 bar, in particular 05. to 1 bar.
11. 11. The process according to one of Claims 7 to 10, characterized in that the cultivation or fermentation is carried out at a temperature of 35 to 42 ° C, in particular at 37 ° C.
12. 12. The procedure according to one of the Claims 7. to 11, characterized in that the cultivation and / or fermentation is carried out with a pH of the order of 6 and 8, in particular with a pH of the order of 7 and 7.5.
13. 13. A process according to one of claims 7 to 12, characterized in that the cultivation and / or fermentation is carried out in a preparation of a medium comprising components selected from: arginine, citrulline, peptone, yeast extract, mixture (s) of fatty acid (s), mineral solution (s), glucose, hemin solution, menadione, vitamin solution, trace elements, reducing agents.
14. The process according to one of Claims 7 to 13, characterized in that 0.1 to 0.5% by weight, in particular 0.2% by weight, of glucose is added at the start of the culture and / or the fermentation.
15. 15. The process according to one of claims 7 to 14, characterized in that preparations of enzyme of the tricothecene detoxifying active microorganism and / or other anaerobic microorganism are added.
16. The process according to one of Claims 7 to 15, characterized in that the pure culture of the microorganism DSM 11798 is obtained from the culture and / or fermentation solution through at least two additional dilution series in the preparation of the medium, in particular by adding L arginine as a growth stimulator.
17. The process according to claim 16, characterized in that, for the fermentation of the pure culture of the preparation of the medium, 1 to 4% by weight of a reducing agent is added, in particular a mixture of cysteine / sodium sulfide / carbonate of sodium in solution.
18. A process according to one of Claims 7 to 17, characterized in that the solution in culture and / or fermentation is conditioned by concentrating, in particular by centrifuging or filtering and / or stabilizing, in particular, by drying by freezing or spray drying or encapsulation.
19. 19. The procedure according to the Claim 18, characterized in that the stabilization is carried out with the addition of a filler or carrier material, such as aluminum silicates, kieselguhrs, carbohydrates, protein hydrolysates, yeasts, PVPP.
20. 20. The use of the microorganism in pure and / or mixed culture (DSM 11798, DSM 11799) for the preparation of an additive for food.
21. The use according to Claim 20, characterized in that the pure and / or mixed culture (DSM 11798 and DSM 11799) is used as a freeze-drying or spray-drying and / or encapsulated immobilization, potentially when added to the material carrier.
22. 22. The food additive for the inactivation of trichothecenes in foods or in the digestive tract of animals, characterized in that the food additive contains a pure and / or mixed culture of a microorganism according to one of Claims 1 to 6 or a microorganism prepared according to one of Claims 7 to 19, in an amount of 105 1012 cells / kg, in particular 107 to 109 cells / kg, per 1000 kg of feed.
23. 23. The food additive according to claim 22, characterized in that it additionally contains a carrier and / or filler material in an amount of the order of 0.5 to 8 kg / 1000 kg, in particular 0.7 to 4 kg / 1000 kg, of the food.
24. The food additive according to claim 23, characterized in that aluminum silicates, kieselguhrs, carbohydrates, sugar alcohols, starch, milk and whey powder, protein hydrolysates, yeasts and / or PVPP are contained as carrier material or filling.
25. 25. The food additive according to any of Claims 22, 23, or 24, characterized in that the food additive consists of a mixture of 1 to 65% by weight, in particular 5 to 50% by weight, of immobilized spray-dried or freeze-dried microorganism and 99 to 35% by weight, in particular 95 to 50% by weight, of carrier and / or filler material.
26. 26. The use of a feed additive according to any of claims 22 to 25 for the inactivation of deoxynivalenol (DON), T-2 toxin, HT-2 toxin, nivalenol, monoacetoxiscirpenol, diacetoxyscirpenol, trichodermol, verrucarin, rorodine, acetyldeoxynivalenol, isotriodermin, hydroxyisotrichdermin, calonectrin, tetraol T-2, triol T-2, deacetylneosolaniol, neosolaniol, acetylneosolaniol, sporotriquiol, trichotriol, sambucinol and culmorin in a food or in the digestive tract of animals.