MX9800333A - Mutantes de pap que exhiben actividad antiviral y/o antifungica en plantas. - Google Patents

Mutantes de pap que exhiben actividad antiviral y/o antifungica en plantas.

Info

Publication number
MX9800333A
MX9800333A MX9800333A MX9800333A MX9800333A MX 9800333 A MX9800333 A MX 9800333A MX 9800333 A MX9800333 A MX 9800333A MX 9800333 A MX9800333 A MX 9800333A MX 9800333 A MX9800333 A MX 9800333A
Authority
MX
Mexico
Prior art keywords
pap
mutants
plants
pap mutants
mature
Prior art date
Application number
MX9800333A
Other languages
English (en)
Other versions
MXPA98000333A (es
Inventor
Nilgun E Tumer
Original Assignee
Univ Rutgers
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/500,611 external-priority patent/US5756322A/en
Priority claimed from US08/500,694 external-priority patent/US5880329A/en
Application filed by Univ Rutgers filed Critical Univ Rutgers
Publication of MX9800333A publication Critical patent/MX9800333A/es
Publication of MXPA98000333A publication Critical patent/MXPA98000333A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

Abstract

Se describen mutantes de PAP que tienen fitotoxicidad reducida comparadas con PAP tipo silvestre y que confieren resistencia de amplio espectro a virus y/o hongos en plantas. Un grupo de mutantes de PAP se caracteriza por lo menos por una substitucion de aminoácidos en la terminacion N de PAP madura, tal como el residuo de glicina 75 o el residuo de ácido glutámico 97 dos grupos de mutantes de PAP adicionales se caracterizan por las truncaciones en la region de terminacion N de PAP madura y las truncaciones o substituciones de aminoácidos en la region de terminacion C de PAP madura respectivamente y un grupo adicional es enzimáticamente inactivo el cual aun exhibe propiedades antigénicas. También se describen moléculas de ADN que codifican las mutantes de PAP, construcciones de ADN de PAP mutantes y semillas y plantas transgénicas que contienen los ADN. Además se describen métodos para identificar mutantes de PAP que tienen fitotoxicidad reducida, así como mutantes de PAP aisladas y purificadas identificadas por el método.
MXPA/A/1998/000333A 1995-07-11 1998-01-09 Mutantes de pap que exhiben actividad antiviral y/o antifungica en plantas MXPA98000333A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/500,611 US5756322A (en) 1995-07-11 1995-07-11 Pokeweed antiviral protein mutants
US08500694 1995-07-11
US08/500,694 US5880329A (en) 1995-07-11 1995-07-11 DNA encoding pokeweed antiviral protein mutants
US08500611 1995-07-11

Publications (2)

Publication Number Publication Date
MX9800333A true MX9800333A (es) 1998-07-31
MXPA98000333A MXPA98000333A (es) 1998-11-09

Family

ID=

Also Published As

Publication number Publication date
US6627736B1 (en) 2003-09-30
CN1190433A (zh) 1998-08-12
WO1997003183A1 (en) 1997-01-30
NZ312942A (en) 1999-02-25
US6137030A (en) 2000-10-24
CA2226342C (en) 2002-09-17
EP0840782A1 (en) 1998-05-13
CA2226342A1 (en) 1997-01-30
AU6488696A (en) 1997-02-10
AU700144B2 (en) 1998-12-24
EP0840782A4 (en) 2002-12-04
BR9609500A (pt) 1999-05-25

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