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Application filed by Ciba Corning Diagnostics CorpfiledCriticalCiba Corning Diagnostics Corp
Priority claimed from PCT/IB1995/000226external-prioritypatent/WO1995027078A1/en
Publication of MXPA96004607ApublicationCriticalpatent/MXPA96004607A/en
Publication of MX9604607ApublicationCriticalpatent/MX9604607A/en
Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
A new method has been developed for conducting a gene probe assay. The preferred technique involves (1) using a gene amplification technique (e.g., PCR) to multiply the gene sequence of interest and (2) using a hybridization-ligation detection methodology, wherein the sequences of probes hybridized to the target sequence allow for separation and detection (e.g., probes might contain a combination of magnetic particles and acridinium esters) to determine if a specific sequence is present,
MX9604607A1995-04-031995-04-03Hibridization-ligation assays for the detection of specific nucleic acid sequences.
MX9604607A
(en)
A method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library. application to the detection of mycobacteria