MX386201B - Ligadores de brazo colgante corto para nucleótidos en aplicaciones de secuenciación. - Google Patents

Ligadores de brazo colgante corto para nucleótidos en aplicaciones de secuenciación.

Info

Publication number
MX386201B
MX386201B MX2019012373A MX2019012373A MX386201B MX 386201 B MX386201 B MX 386201B MX 2019012373 A MX2019012373 A MX 2019012373A MX 2019012373 A MX2019012373 A MX 2019012373A MX 386201 B MX386201 B MX 386201B
Authority
MX
Mexico
Prior art keywords
ligators
nucleotides
sequencing applications
compounds
arm
Prior art date
Application number
MX2019012373A
Other languages
English (en)
Inventor
Antoine Francais
Elena Cressina
Xiaohai Liu
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of MX386201B publication Critical patent/MX386201B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/14—Pyrrolo-pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Chemical Kinetics & Catalysis (AREA)

Abstract

La presente invención se refiere a nuevos compuestos de nucleótido y oligonucleótido y su uso en aplicaciones de secuenciación de ácido nucleico.
MX2019012373A 2017-07-12 2018-07-12 Ligadores de brazo colgante corto para nucleótidos en aplicaciones de secuenciación. MX386201B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1711219.4A GB201711219D0 (en) 2017-07-12 2017-07-12 Short pendant arm linkers for nucleotides in sequencing applications
PCT/EP2018/069030 WO2019012080A1 (en) 2017-07-12 2018-07-12 SHORT ARM LINKS FOR NUCLEOTIDES IN SEQUENCING APPLICATIONS

Publications (1)

Publication Number Publication Date
MX386201B true MX386201B (es) 2025-03-18

Family

ID=59676794

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2019012373A MX386201B (es) 2017-07-12 2018-07-12 Ligadores de brazo colgante corto para nucleótidos en aplicaciones de secuenciación.
MX2021011019A MX2021011019A (es) 2017-07-12 2019-10-16 Ligadores de brazo colgante corto para nucleotidos en aplicaciones de secuenciacion.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2021011019A MX2021011019A (es) 2017-07-12 2019-10-16 Ligadores de brazo colgante corto para nucleotidos en aplicaciones de secuenciacion.

Country Status (14)

Country Link
US (4) US10526648B2 (es)
EP (1) EP3652191A1 (es)
JP (1) JP7041695B2 (es)
KR (2) KR20220119182A (es)
CN (1) CN111094315B (es)
AU (1) AU2018298847B2 (es)
CA (1) CA3060152A1 (es)
GB (1) GB201711219D0 (es)
IL (1) IL271428A (es)
MX (2) MX386201B (es)
MY (1) MY196912A (es)
RU (1) RU2019137083A (es)
WO (1) WO2019012080A1 (es)
ZA (1) ZA201906836B (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
SG11202012559SA (en) 2018-12-05 2021-01-28 Illumina Inc Polymerases, compositions, and methods of use
CN119662792A (zh) * 2019-02-19 2025-03-21 阿尔缇玛基因组学公司 用于光学检测和测序的接头和方法
AU2020233166B2 (en) 2019-03-01 2025-11-13 Illumina Cambridge Limited Tertiary amine substituted coumarin compounds and their uses as fluorescent labels
CA3103900A1 (en) 2019-03-01 2020-09-10 Illumina Cambridge Limited Exocyclic amine substituted coumarin compounds and their uses as fluorescent labels
JP2023510454A (ja) 2019-11-27 2023-03-14 イルミナ ケンブリッジ リミテッド シクロオクタテトラエンを含有する色素及び組成物
CN115197289B (zh) * 2021-04-01 2025-03-07 深圳华大生命科学研究院 用于测序的核苷酸类似物
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
CN116239493B (zh) * 2023-03-15 2024-07-26 杭州易速微控基因技术有限公司 一种Linker类化合物的合成方法

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268486A (en) 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
US6465178B2 (en) 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
ATE309390T1 (de) 1998-07-30 2005-11-15 Solexa Ltd Matrizen von biomolekülen und ihre verwendung in sequenzierung
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6391937B1 (en) 1998-11-25 2002-05-21 Motorola, Inc. Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
US6664061B2 (en) 1999-06-25 2003-12-16 Amersham Biosciences Ab Use and evaluation of a [2+2] photoaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
WO2002012566A2 (en) 2000-08-09 2002-02-14 Motorola, Inc. The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
EP2325263B1 (en) 2000-09-29 2013-01-23 Life Technologies Corporation Modified carbocyanine dyes and their conjugates
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
AU1312502A (en) 2000-10-11 2002-04-22 Pe Corp Ny Fluorescent nucleobase conjugates having anionic linkers
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
JP2006509040A (ja) 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
US11008359B2 (en) * 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
DE10258150A1 (de) 2002-12-10 2004-07-08 Dyomics Gmbh Hydrophile Marker auf der Basis von Benzopyrylo-Polymethinen
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
GB0326073D0 (en) 2003-11-07 2003-12-10 Solexa Ltd Improvements in or relating to polynucleotide arrays
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
WO2006097320A2 (de) * 2005-03-17 2006-09-21 Genovoxx Gmbh Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
US8212020B2 (en) 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
JP5513122B2 (ja) 2006-12-05 2014-06-04 レーザーゲン インコーポレイテッド 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
EP2652153B1 (en) 2010-12-17 2017-07-05 The Trustees of Columbia University in the City of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
ES2620427T3 (es) 2013-03-08 2017-06-28 Illumina Cambridge Limited Compuestos rodamina y su uso como marcadores fluorescentes
EP3074350A4 (en) 2013-11-25 2017-08-09 University Of Idaho Biochar water treatment
WO2017185026A1 (en) 2016-04-22 2017-10-26 Complete Genomics, Inc. Reversibly blocked nucleoside analogues and their use
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications

Also Published As

Publication number Publication date
ZA201906836B (en) 2023-04-26
KR20220119182A (ko) 2022-08-26
US20200131569A1 (en) 2020-04-30
JP7041695B2 (ja) 2022-03-24
IL271428A (en) 2020-01-30
US11655502B2 (en) 2023-05-23
US20230348968A1 (en) 2023-11-02
WO2019012080A1 (en) 2019-01-17
CA3060152A1 (en) 2019-01-17
JP2020527131A (ja) 2020-09-03
US20210230687A1 (en) 2021-07-29
EP3652191A1 (en) 2020-05-20
BR112019027614A2 (pt) 2020-07-07
US10526648B2 (en) 2020-01-07
US20190017111A1 (en) 2019-01-17
MY196912A (en) 2023-05-10
AU2018298847A1 (en) 2019-11-07
KR20200023282A (ko) 2020-03-04
CN111094315A (zh) 2020-05-01
RU2019137083A3 (es) 2021-06-18
GB201711219D0 (en) 2017-08-23
RU2019137083A (ru) 2021-06-18
CN111094315B (zh) 2024-04-09
AU2018298847B2 (en) 2021-02-11
US12071662B2 (en) 2024-08-27
US11001888B2 (en) 2021-05-11
NZ759350A (en) 2023-10-27
MX2021011019A (es) 2021-10-14

Similar Documents

Publication Publication Date Title
MX386201B (es) Ligadores de brazo colgante corto para nucleótidos en aplicaciones de secuenciación.
MX2020013384A (es) Compuestos de comarina sustituidos con amina exocíclica y sus usos como etiquetas fluorescentes.
BR112016010165A2 (pt) composições de nucleotídeos modificados de citidina-5-carboxamida e métodos relacionados às mesmas
CY1125029T1 (el) Τροποποιημενοι νουκλεοζιτες, νουκλεοτιδια, και νουκλεϊκα οξεα και χρησεις αυτων
CY1125421T1 (el) Ριβονουκλεϊκα οξεα που περιεχουν ν1-μεθυλο- ψευδοουρακιλες και χρησεις αυτων
NZ740630A (en) Polymethine compounds and their use as fluorescent labels
EA201592075A1 (ru) Замещенные нуклеозиды, нуклеотиды и их аналоги
NZ703824A (en) Oligonucleotides for making a change in the sequence of a target rna molecule present in a living cell
CL2013003053A1 (es) Compuesto que comprende un oligonucleotido modificado que consiste en de 10 a 30 nucleósidos unidos y definidos por un grupo de secuencias específicas; composicion que comprende dicho compuesto; uso para tratar enfermedades relacionadas con el virus de la hepatitis b (vhb).
EA201390230A1 (ru) Замещенные аналоги нуклеотидов
EA201791417A1 (ru) Супрессия гена гентингтина, индуцированная рнк-интерференцией
ECSP15031144A (es) Nucleósidos sustituidos, nucleótidos y análogos de los mismos
PE20191351A1 (es) Tratamiento aav de la enfermedad de huntington
EA201690526A1 (ru) Замещенные нуклеозиды, нуклеотиды и их аналоги
EA201492123A1 (ru) Композиции и способы для модулирования экспрессии семейства генов smn
EA201490993A1 (ru) МОДИФИЦИРОВАННЫЕ СРЕДСТВА РНКи
HK1213018A1 (zh) 橋接雙環核苷
MX374553B (es) Derivado de ácido nucleico que tiene actividad inmunoestimuladora.
EA034882B9 (ru) Способ синтеза полинуклеотида
MX389717B (es) COMPOSICIONES Y MÉTODOS PARA CONSTRUIR GENOTECAS DE ADNc ESPECÍFICAS DE HEBRA.
BR112018017316A2 (pt) compostos e métodos para a síntese de 5-(n-protegido-triptaminocarboxiamida)-2?-desoxiuridina fosforamidato para incorporação em uma sequência nucleica
ECSP11011167A (es) Síntesis y formas de sales novedosas de (r) -5- ( (e) -2- (pirrolidin-3-ilvinil) pirimidina
MY186779A (en) Nucleic acid derivative having immunostimulatory activity
EP3283501A4 (en) STOCHIOMETRIC VOTING OF NUCLEIC ACID HYBRIDIZATION SOLIDS BY AUXILIARY OLIGONUCLEOTIDE SPECIES
RU2013135816A (ru) Набор олигонуклеотидных праймеров и флуоресцентномеченого зонда для видоспецифичной экспресс-идентификации рнк вируса ласса методом полимеразной цепной реакции в реальном времени