MX2023000035A - Método de tratamiento de edición del gen de la deficiencia de piruvato quinasa (pkd). - Google Patents
Método de tratamiento de edición del gen de la deficiencia de piruvato quinasa (pkd).Info
- Publication number
- MX2023000035A MX2023000035A MX2023000035A MX2023000035A MX2023000035A MX 2023000035 A MX2023000035 A MX 2023000035A MX 2023000035 A MX2023000035 A MX 2023000035A MX 2023000035 A MX2023000035 A MX 2023000035A MX 2023000035 A MX2023000035 A MX 2023000035A
- Authority
- MX
- Mexico
- Prior art keywords
- pkd
- pyruvate kinase
- kinase deficiency
- treatment method
- gene editing
- Prior art date
Links
- 108700014121 Pyruvate Kinase Deficiency of Red Cells Proteins 0.000 title abstract 3
- 238000010362 genome editing Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 230000005782 double-strand break Effects 0.000 abstract 2
- 230000006801 homologous recombination Effects 0.000 abstract 2
- 238000002744 homologous recombination Methods 0.000 abstract 2
- 108091033409 CRISPR Proteins 0.000 abstract 1
- 108010040467 CRISPR-Associated Proteins Proteins 0.000 abstract 1
- 108020005004 Guide RNA Proteins 0.000 abstract 1
- 101150025052 Pklr gene Proteins 0.000 abstract 1
- 108091028113 Trans-activating crRNA Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/0104—Pyruvate kinase (2.7.1.40)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/30—Special therapeutic applications
- C12N2320/34—Allele or polymorphism specific uses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/50—Biochemical production, i.e. in a transformed host cell
- C12N2330/51—Specially adapted vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
La presente invención se refiere al tratamiento de deficiencia de piruvato quinasa (PKD) utilizando el sistema de repeticiones palindrómicas cortas agrupadas interespaciadas de manera regular (CRISPR). Esta tecnología ofrece la posibilidad de diseñar un ARN guía individual (sgRNA) mejorado, en particular, un crRNA mejorado para ser asociado con tracrRNA, el cual se incorpora en una proteína asociada con CRISPR (Cas9) para reconocer e inducir rompimientos de cadena doble de ADN en una ubicación objetivo específica. Los rompimientos de cadena doble de ADN van a ser reparados mediante recombinación homóloga (HR) en presencia de una secuencia donadora para la reparación del gen de PKLR.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP20382568.2A EP3929294A1 (en) | 2020-06-26 | 2020-06-26 | Pyruvate kinase deficiency (pkd) gene editing treatment method |
PCT/EP2021/067719 WO2021260227A1 (en) | 2020-06-26 | 2021-06-28 | Pyruvate kinase deficiency (pkd) gene editing treatment method |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2023000035A true MX2023000035A (es) | 2023-04-10 |
Family
ID=71607853
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2023000035A MX2023000035A (es) | 2020-06-26 | 2021-06-28 | Método de tratamiento de edición del gen de la deficiencia de piruvato quinasa (pkd). |
Country Status (9)
Country | Link |
---|---|
US (1) | US20230303990A1 (es) |
EP (2) | EP3929294A1 (es) |
JP (1) | JP2023531718A (es) |
KR (1) | KR20230029922A (es) |
CN (1) | CN116648511A (es) |
AU (1) | AU2021298186A1 (es) |
CA (1) | CA3188164A1 (es) |
MX (1) | MX2023000035A (es) |
WO (1) | WO2021260227A1 (es) |
-
2020
- 2020-06-26 EP EP20382568.2A patent/EP3929294A1/en not_active Withdrawn
-
2021
- 2021-06-28 AU AU2021298186A patent/AU2021298186A1/en active Pending
- 2021-06-28 WO PCT/EP2021/067719 patent/WO2021260227A1/en active Application Filing
- 2021-06-28 EP EP21734023.1A patent/EP4172332A1/en active Pending
- 2021-06-28 US US18/012,894 patent/US20230303990A1/en active Pending
- 2021-06-28 CN CN202180053301.9A patent/CN116648511A/zh active Pending
- 2021-06-28 JP JP2022579942A patent/JP2023531718A/ja active Pending
- 2021-06-28 KR KR1020237003103A patent/KR20230029922A/ko active Search and Examination
- 2021-06-28 CA CA3188164A patent/CA3188164A1/en active Pending
- 2021-06-28 MX MX2023000035A patent/MX2023000035A/es unknown
Also Published As
Publication number | Publication date |
---|---|
EP4172332A1 (en) | 2023-05-03 |
CN116648511A (zh) | 2023-08-25 |
AU2021298186A1 (en) | 2023-02-09 |
KR20230029922A (ko) | 2023-03-03 |
US20230303990A1 (en) | 2023-09-28 |
EP3929294A1 (en) | 2021-12-29 |
JP2023531718A (ja) | 2023-07-25 |
CA3188164A1 (en) | 2021-12-30 |
WO2021260227A1 (en) | 2021-12-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Song et al. | Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger | |
US10301613B2 (en) | Targeted remodeling of prokaryotic genomes using CRISPR-nickases | |
Waaijers et al. | Engineering the Caenorhabditis elegans genome with CRISPR/Cas9 | |
MX2022008197A (es) | Metodo para el tratamiento del sindrome de usher y composicion del mismo. | |
RU2707542C1 (ru) | Способ получения препарата рекомбинантной нуклеазы CAS, по существу, свободного от бактериальных эндотоксинов, полученный данным способом препарат и содержащий его набор для использования в системе CRISPR/Cas | |
US20190352670A1 (en) | Methods for Increasing Efficiency of Gene Editing in Cells | |
WO2019094791A3 (en) | Targeted crispr delivery platforms | |
EP3464587A1 (en) | Compositions and methods for enhancing homologous recombination | |
CN104560995A (zh) | 一对特异识别猪H11位点的sgRNA及其编码DNA和应用 | |
Ublinskaya et al. | A PCR-free cloning method for the targeted φ80 Int-mediated integration of any long DNA fragment, bracketed with meganuclease recognition sites, into the Escherichia coli chromosome | |
EP4038192A2 (en) | Genetic engineering of fungi to modulate tryptamine expression | |
Zhang et al. | CRISPR/Cas9 genome editing: a promising tool for therapeutic applications of induced pluripotent stem cells | |
CO2023016031A2 (es) | Composiciones y métodos para el direccionamiento genético in vivo mediado por nucleasas para el tratamiento de trastornos genéticos | |
Onuma et al. | Efficient mutagenesis by CRISPR/Cas system during meiotic maturation of porcine oocytes | |
MX2023000035A (es) | Método de tratamiento de edición del gen de la deficiencia de piruvato quinasa (pkd). | |
EP3412765B1 (en) | Method for producing mutant filamentous fungi | |
MX2022010835A (es) | Recombinacion del genoma guiada por arn en escala de kilobase. | |
WO2021257997A3 (en) | Crispr-associated transposase systems and methods of use thereof | |
MX2022008937A (es) | Edicion genica para el tratamiento de epidermolisis ampollosa. | |
CN112111528B (zh) | 一种内含子异常剪接的修复方法 | |
Khatodia et al. | Trending: the Cas nuclease mediated genome editing technique | |
CN112391410B (zh) | 一种sgRNA及其在修复内含子异常剪接中的应用 | |
Yi et al. | CRISPR-based m6A modification and its potential applications in telomerase regulation | |
Jambhulkar et al. | Genome Editing Techniques and Their Applications in Brassica Crop Improvement | |
AR125466A1 (es) | Composiciones y métodos de inserción dirigida de genes in vivo mediada por nucleasa para el tratamiento de trastornos genéticos |