MX2022014718A - Un metodo para calibrar la eficiencia de siembra de genotecas de ácidos nucleicos en celdas de flujo. - Google Patents
Un metodo para calibrar la eficiencia de siembra de genotecas de ácidos nucleicos en celdas de flujo.Info
- Publication number
- MX2022014718A MX2022014718A MX2022014718A MX2022014718A MX2022014718A MX 2022014718 A MX2022014718 A MX 2022014718A MX 2022014718 A MX2022014718 A MX 2022014718A MX 2022014718 A MX2022014718 A MX 2022014718A MX 2022014718 A MX2022014718 A MX 2022014718A
- Authority
- MX
- Mexico
- Prior art keywords
- calibrate
- flowcells
- nucleic acid
- acid library
- seeding efficiency
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000010899 nucleation Methods 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
- C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
- C12Q2539/101—Subtraction analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2545/00—Reactions characterised by their quantitative nature
- C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
- C12Q2545/114—Reactions characterised by their quantitative nature the purpose being quantitative analysis involving a quantitation step
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Abstract
La descripción proporciona métodos para calibrar la eficiencia de la siembra de polinucleótidos en celdas de flujo.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202063047817P | 2020-07-02 | 2020-07-02 | |
PCT/US2021/040245 WO2022006495A1 (en) | 2020-07-02 | 2021-07-02 | A method to calibrate nucleic acid library seeding efficiency in flowcells |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2022014718A true MX2022014718A (es) | 2023-03-06 |
Family
ID=77838906
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2022014718A MX2022014718A (es) | 2020-07-02 | 2021-07-02 | Un metodo para calibrar la eficiencia de siembra de genotecas de ácidos nucleicos en celdas de flujo. |
Country Status (11)
Country | Link |
---|---|
US (1) | US20230242967A1 (es) |
EP (1) | EP4176081A1 (es) |
JP (1) | JP2023532079A (es) |
KR (1) | KR20230035253A (es) |
CN (1) | CN115843315A (es) |
AU (1) | AU2021300252A1 (es) |
BR (1) | BR112022026806A2 (es) |
CA (1) | CA3182091A1 (es) |
IL (1) | IL299052A (es) |
MX (1) | MX2022014718A (es) |
WO (1) | WO2022006495A1 (es) |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0450060A1 (en) | 1989-10-26 | 1991-10-09 | Sri International | Dna sequencing |
DE69837913T2 (de) | 1997-04-01 | 2008-02-07 | Solexa Ltd., Saffron Walden | Verfahren zur vervielfältigung von nukleinsäure |
AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
WO2002004680A2 (en) | 2000-07-07 | 2002-01-17 | Visigen Biotechnologies, Inc. | Real-time sequence determination |
US7211414B2 (en) | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
AR031640A1 (es) | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
EP3795577A1 (en) | 2002-08-23 | 2021-03-24 | Illumina Cambridge Limited | Modified nucleotides |
JP2008513782A (ja) | 2004-09-17 | 2008-05-01 | パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド | 分子解析のための装置及び方法 |
US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
EP3373174A1 (en) | 2006-03-31 | 2018-09-12 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
AU2007309504B2 (en) | 2006-10-23 | 2012-09-13 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
WO2012061832A1 (en) | 2010-11-05 | 2012-05-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
WO2012170936A2 (en) | 2011-06-09 | 2012-12-13 | Illumina, Inc. | Patterned flow-cells useful for nucleic acid analysis |
EP2771103B1 (en) | 2011-10-28 | 2017-08-16 | Illumina, Inc. | Microarray fabrication system and method |
GB201410420D0 (en) * | 2014-06-11 | 2014-07-23 | Illumina Cambridge Ltd | Methods for estimating cluster numbers |
CN109313396B (zh) | 2016-05-18 | 2022-11-22 | Illumina公司 | 使用图案化疏水性表面进行的自组装图案化 |
WO2018161019A1 (en) * | 2017-03-03 | 2018-09-07 | Counsyl, Inc. | Methods for optimizing direct targeted sequencing |
-
2021
- 2021-07-02 CA CA3182091A patent/CA3182091A1/en active Pending
- 2021-07-02 AU AU2021300252A patent/AU2021300252A1/en active Pending
- 2021-07-02 WO PCT/US2021/040245 patent/WO2022006495A1/en active Application Filing
- 2021-07-02 JP JP2022580775A patent/JP2023532079A/ja active Pending
- 2021-07-02 BR BR112022026806A patent/BR112022026806A2/pt unknown
- 2021-07-02 KR KR1020227045429A patent/KR20230035253A/ko unknown
- 2021-07-02 IL IL299052A patent/IL299052A/en unknown
- 2021-07-02 US US18/013,788 patent/US20230242967A1/en active Pending
- 2021-07-02 EP EP21773424.3A patent/EP4176081A1/en active Pending
- 2021-07-02 MX MX2022014718A patent/MX2022014718A/es unknown
- 2021-07-02 CN CN202180046851.8A patent/CN115843315A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
BR112022026806A2 (pt) | 2023-04-25 |
CN115843315A (zh) | 2023-03-24 |
WO2022006495A1 (en) | 2022-01-06 |
CA3182091A1 (en) | 2022-01-06 |
US20230242967A1 (en) | 2023-08-03 |
IL299052A (en) | 2023-02-01 |
EP4176081A1 (en) | 2023-05-10 |
JP2023532079A (ja) | 2023-07-26 |
AU2021300252A1 (en) | 2023-01-05 |
WO2022006495A8 (en) | 2023-05-04 |
KR20230035253A (ko) | 2023-03-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP4270012A3 (en) | Automated cell processing methods, modules, instruments and systems | |
MX2016002118A (es) | Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso. | |
EP3699280A3 (en) | Novel cas9 systems and methods of use | |
UA103887C2 (ru) | Способ получения стойкого к гербицидам растения | |
NZ738689A (en) | Engineered crispr-cas9 compositions and methods of use | |
MX2018004263A (es) | Edición genómica multiplexada. | |
TN2014000493A1 (en) | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription | |
EP3418379A3 (en) | Methods, cells & organisms | |
EP3722438A3 (en) | Method for production of moth pheromones in yeast | |
WO2010016943A3 (en) | Nutrient monitoring and feedback control for increased bioproduct production | |
MY183741A (en) | Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair | |
AR076473A1 (es) | Manipulacion de la biosintesis de fructano y aumento de biomasa en plantas | |
WO2014138119A3 (en) | Method and kit for characterizing microorganisms | |
GB2548740A (en) | Methods for generation of podocytes from pluripotent stem cells and cells produced by the same | |
MX2009003616A (es) | Secuencias de microarn del maiz. | |
WO2015150716A3 (fr) | Procede de culture des microalgues du genre aurantiochytrium dans un milieu de culture sans chlorure et sans sodium pour la production de dha | |
MX2021008920A (es) | Un método de edición de genes. | |
MX2016000557A (es) | Conversion basada en moleculas pequeñas de celulas somaticas en celulas de cresta neural. | |
WO2015073625A3 (en) | Synergistic genome-nonintegrating reprogramming by micrornas and transcription factors | |
EA201491785A1 (ru) | Способ ферментации | |
WO2014144987A3 (en) | Targeted gene modification using oligonucleotide-mediated gene repair | |
MX2022014718A (es) | Un metodo para calibrar la eficiencia de siembra de genotecas de ácidos nucleicos en celdas de flujo. | |
SG11201811735WA (en) | Method for producing dna library and method for analyzing genomic dna using the dna library | |
MX2019006635A (es) | Produccion de manool. | |
EP2480657A4 (en) | METHOD OF EFFICIENTLY ESTABLISHING A LINE OF INDUCED PLURIPOTENT STEM CELLS (SPI) |