MX2022014188A - Captura selectiva de secuencias blanco de adn. - Google Patents
Captura selectiva de secuencias blanco de adn.Info
- Publication number
- MX2022014188A MX2022014188A MX2022014188A MX2022014188A MX2022014188A MX 2022014188 A MX2022014188 A MX 2022014188A MX 2022014188 A MX2022014188 A MX 2022014188A MX 2022014188 A MX2022014188 A MX 2022014188A MX 2022014188 A MX2022014188 A MX 2022014188A
- Authority
- MX
- Mexico
- Prior art keywords
- regions
- capture
- baits
- similar
- dna sequences
- Prior art date
Links
- 108091028043 Nucleic acid sequence Proteins 0.000 title 1
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract 2
- 230000000903 blocking effect Effects 0.000 abstract 2
- 238000012163 sequencing technique Methods 0.000 abstract 2
- 229960002685 biotin Drugs 0.000 abstract 1
- 235000020958 biotin Nutrition 0.000 abstract 1
- 239000011616 biotin Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/519—Detection characterised by immobilisation to a surface characterised by the capture moiety being a single stranded oligonucleotide
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Muchas regiones de ADN genómico son altamente similares a otras regiones del genoma y así son muy difíciles de capturar sin también capturar las regiones no deseadas, similares. Esto conduce a sobre-secuenciación de regiones para las cuales no existe interés y disminuye la cobertura de las regiones deseadas. Para minimizar la captura de regiones no deseadas, los cebos de bloqueo se han diseñado para evitar que se capturen fragmentos similares, pero no deseados. Esto permite secuenciación más dirigida de las regiones de interés. Los cebos de bloqueo difieren de los cebos de captura porque tienen una secuencia modestamente diferente que se unen de preferencia al ADN no deseado y no contienen una biotina u otra modificación así que permanecen detrás cuando se seleccionan los cebos de captura.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063022968P | 2020-05-11 | 2020-05-11 | |
| PCT/US2021/031623 WO2021231324A1 (en) | 2020-05-11 | 2021-05-10 | Selective capture of target dna sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2022014188A true MX2022014188A (es) | 2023-02-22 |
Family
ID=78412321
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2022014188A MX2022014188A (es) | 2020-05-11 | 2021-05-10 | Captura selectiva de secuencias blanco de adn. |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US11655503B2 (es) |
| EP (1) | EP4150110A1 (es) |
| JP (1) | JP2023525286A (es) |
| KR (1) | KR20230034950A (es) |
| CN (1) | CN116323963A (es) |
| AU (1) | AU2021271628A1 (es) |
| BR (1) | BR112022023011A2 (es) |
| CA (1) | CA3182271A1 (es) |
| GB (1) | GB2611205A (es) |
| MX (1) | MX2022014188A (es) |
| WO (1) | WO2021231324A1 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230140482A (ko) | 2022-03-22 | 2023-10-06 | 주식회사 엘지에너지솔루션 | 전지 셀에서의 리튬 석출 검사 장치 및 방법 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8632970B2 (en) | 2005-05-09 | 2014-01-21 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
| US10577643B2 (en) * | 2015-10-07 | 2020-03-03 | Illumina, Inc. | Off-target capture reduction in sequencing techniques |
| AU2017363204B2 (en) | 2016-11-18 | 2022-12-08 | Twist Bioscience Corporation | Polynucleotide libraries having controlled stoichiometry and synthesis thereof |
-
2021
- 2021-05-10 CA CA3182271A patent/CA3182271A1/en active Pending
- 2021-05-10 AU AU2021271628A patent/AU2021271628A1/en active Pending
- 2021-05-10 MX MX2022014188A patent/MX2022014188A/es unknown
- 2021-05-10 JP JP2022567782A patent/JP2023525286A/ja active Pending
- 2021-05-10 CN CN202180034569.8A patent/CN116323963A/zh active Pending
- 2021-05-10 US US17/316,566 patent/US11655503B2/en active Active
- 2021-05-10 BR BR112022023011A patent/BR112022023011A2/pt unknown
- 2021-05-10 KR KR1020227042876A patent/KR20230034950A/ko active Search and Examination
- 2021-05-10 WO PCT/US2021/031623 patent/WO2021231324A1/en unknown
- 2021-05-10 EP EP21804722.3A patent/EP4150110A1/en active Pending
- 2021-05-10 GB GB2218135.8A patent/GB2611205A/en active Pending
-
2023
- 2023-04-14 US US18/300,751 patent/US20240191289A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2021231324A1 (en) | 2021-11-18 |
| BR112022023011A2 (pt) | 2022-12-20 |
| AU2021271628A1 (en) | 2022-11-10 |
| US11655503B2 (en) | 2023-05-23 |
| KR20230034950A (ko) | 2023-03-10 |
| EP4150110A1 (en) | 2023-03-22 |
| GB202218135D0 (en) | 2023-01-18 |
| US20210348225A1 (en) | 2021-11-11 |
| JP2023525286A (ja) | 2023-06-15 |
| CA3182271A1 (en) | 2021-11-18 |
| CN116323963A (zh) | 2023-06-23 |
| GB2611205A (en) | 2023-03-29 |
| US20240191289A1 (en) | 2024-06-13 |
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