MX2022005589A - Nuevo proceso para extraer proteína. - Google Patents

Nuevo proceso para extraer proteína.

Info

Publication number
MX2022005589A
MX2022005589A MX2022005589A MX2022005589A MX2022005589A MX 2022005589 A MX2022005589 A MX 2022005589A MX 2022005589 A MX2022005589 A MX 2022005589A MX 2022005589 A MX2022005589 A MX 2022005589A MX 2022005589 A MX2022005589 A MX 2022005589A
Authority
MX
Mexico
Prior art keywords
cell suspension
temperature
cytoplasmic
periplasmic protein
aqueous solution
Prior art date
Application number
MX2022005589A
Other languages
English (en)
Inventor
Finn Dunås
Belinda Röjsäter
Tomas Moks
Original Assignee
Affibody Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Affibody Ab filed Critical Affibody Ab
Publication of MX2022005589A publication Critical patent/MX2022005589A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un método para extraer una proteína citoplasmática o periplásmica. El método comprende la provisión de una primera suspensión de células que comprende células, las células que contienen una proteína citoplasmática o periplásmica de interés que se va a extraer, la primera suspensión de células que tiene una primera temperatura, y el calentamiento de la primera suspensión de células a una temperatura de operación, la temperatura de operación a la cual al menos una fracción de las células se somete a lisis inducida por calor y al menos una fracción de la proteína citoplasmática o periplásmica de interés que se va a extraer no se somete a desnaturalización irreversible. El calentamiento de la primera suspensión de células comprende la provisión de una solución acuosa, la solución acuosa que tiene una segunda temperatura que es mayor que la primera temperatura, y la mezcla de la primera suspensión de células con la solución acuosa, obteniendo de este modo una segunda suspensión de células, la segunda suspensión de células que tiene una tercera temperatura que es mayor que la primera temperatura. Un sistema para extraer una proteína citoplasmática o periplásmica. El sistema comprende, entre otras cosas, una mezcladora estática.
MX2022005589A 2019-11-08 2020-11-09 Nuevo proceso para extraer proteína. MX2022005589A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19208153 2019-11-08
PCT/EP2020/081420 WO2021089862A1 (en) 2019-11-08 2020-11-09 New process of extracting protein

Publications (1)

Publication Number Publication Date
MX2022005589A true MX2022005589A (es) 2022-08-22

Family

ID=68501529

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022005589A MX2022005589A (es) 2019-11-08 2020-11-09 Nuevo proceso para extraer proteína.

Country Status (11)

Country Link
US (1) US20220411743A1 (es)
EP (1) EP4055027A1 (es)
JP (1) JP2023500856A (es)
KR (1) KR20220122982A (es)
CN (1) CN114616237A (es)
AU (1) AU2020380524A1 (es)
BR (1) BR112022008618A2 (es)
CA (1) CA3160027A1 (es)
IL (1) IL292533A (es)
MX (1) MX2022005589A (es)
WO (1) WO2021089862A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114395029B (zh) * 2022-03-03 2023-07-14 天津鸿宇泰生物科技有限公司 一种大肠杆菌细胞周质蛋白的提取方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197553B1 (en) * 1994-07-15 2001-03-06 Merck & Co., Inc. Method for large scale plasmid purification
AU2006268111A1 (en) * 2005-07-13 2007-01-18 Amgen Mountain View Inc. Il-6 binding proteins
US8211668B2 (en) * 2007-01-12 2012-07-03 Dow Agrosciences, Llc. Apparatus and methods for osmotically shocking cells
GB201103138D0 (en) * 2011-02-23 2011-04-06 Msd Biolog Uk Ltd Cell lysis process
MX2016011970A (es) * 2014-03-18 2016-12-05 Roquette Freres Metodo para la permeabilizacion termica de una biomasa de microalgas.

Also Published As

Publication number Publication date
EP4055027A1 (en) 2022-09-14
IL292533A (en) 2022-06-01
JP2023500856A (ja) 2023-01-11
WO2021089862A1 (en) 2021-05-14
CA3160027A1 (en) 2021-05-14
CN114616237A (zh) 2022-06-10
AU2020380524A1 (en) 2022-06-02
US20220411743A1 (en) 2022-12-29
KR20220122982A (ko) 2022-09-05
BR112022008618A2 (pt) 2022-07-19

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