MX2021013191A - Method and process for in vitro decellularization of cardiovascular tissue. - Google Patents

Method and process for in vitro decellularization of cardiovascular tissue.

Info

Publication number
MX2021013191A
MX2021013191A MX2021013191A MX2021013191A MX2021013191A MX 2021013191 A MX2021013191 A MX 2021013191A MX 2021013191 A MX2021013191 A MX 2021013191A MX 2021013191 A MX2021013191 A MX 2021013191A MX 2021013191 A MX2021013191 A MX 2021013191A
Authority
MX
Mexico
Prior art keywords
tissue
cardiovascular tissue
recirculation system
decellularization
vitro
Prior art date
Application number
MX2021013191A
Other languages
Spanish (es)
Inventor
Alemán Juan Pablo Aguilar
Pérez Beni Camacho
García Octavio Israel Rojas
Chávez Omar Peza
Original Assignee
Top Health S A P I De C V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Top Health S A P I De C V filed Critical Top Health S A P I De C V
Priority to MX2021013191A priority Critical patent/MX2021013191A/en
Priority to PCT/MX2021/050071 priority patent/WO2023075586A1/en
Publication of MX2021013191A publication Critical patent/MX2021013191A/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Transplantation (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Cardiology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The present invention refers to a method and process for in vitro decellularization of cardiovascular tissue, such as heart valves, pulmonary arteries, large vessels, veins, and peripheral arterial networks. It consists of a) subjecting the cardiovascular tissue to a recirculation system of Decellularizing hypotonic solutions in a stepwise concentration gradient to lyse cells from the plasma membrane but keeping the extracellular matrix intact, b) subjecting the cardiovascular tissue to a recirculation system of nucleic acid degrading enzymes to eliminate cellular debris in the tissue, and c) submitting the cardiovascular tissue to a recirculation system of isotonic solutions to remove toxic residues and preserve the structural integrity of the tissue.
MX2021013191A 2021-10-27 2021-10-27 Method and process for in vitro decellularization of cardiovascular tissue. MX2021013191A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MX2021013191A MX2021013191A (en) 2021-10-27 2021-10-27 Method and process for in vitro decellularization of cardiovascular tissue.
PCT/MX2021/050071 WO2023075586A1 (en) 2021-10-27 2021-10-29 Method and process for in vitro decellularisation of cardiovascular tissue

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX2021013191A MX2021013191A (en) 2021-10-27 2021-10-27 Method and process for in vitro decellularization of cardiovascular tissue.

Publications (1)

Publication Number Publication Date
MX2021013191A true MX2021013191A (en) 2023-04-28

Family

ID=86160131

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021013191A MX2021013191A (en) 2021-10-27 2021-10-27 Method and process for in vitro decellularization of cardiovascular tissue.

Country Status (2)

Country Link
MX (1) MX2021013191A (en)
WO (1) WO2023075586A1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6734018B2 (en) * 1999-06-07 2004-05-11 Lifenet Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced
US20110165676A1 (en) * 2009-11-06 2011-07-07 The Children's Mercy Hospital Method for decellularization
US11590261B2 (en) * 2014-04-02 2023-02-28 Biotronik Ag Method for the preparation of biological tissue for dry use in an implant
GB2543540B (en) * 2015-10-21 2020-10-14 Videregen Ltd Decellularising tissue

Also Published As

Publication number Publication date
WO2023075586A1 (en) 2023-05-04

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