MX2019010007A - Métodos para generar y separar por exclusión genotecas de péptidos compartimentados. - Google Patents

Métodos para generar y separar por exclusión genotecas de péptidos compartimentados.

Info

Publication number
MX2019010007A
MX2019010007A MX2019010007A MX2019010007A MX2019010007A MX 2019010007 A MX2019010007 A MX 2019010007A MX 2019010007 A MX2019010007 A MX 2019010007A MX 2019010007 A MX2019010007 A MX 2019010007A MX 2019010007 A MX2019010007 A MX 2019010007A
Authority
MX
Mexico
Prior art keywords
compartmentalised
polypeptide
methods
screening
polynucleotide
Prior art date
Application number
MX2019010007A
Other languages
English (en)
Inventor
Tavassoli Ali
SOHRABI Catrin
Fischlechner Martin
Original Assignee
Univ Southampton
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Southampton filed Critical Univ Southampton
Publication of MX2019010007A publication Critical patent/MX2019010007A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/64—Cyclic peptides containing only normal peptide links
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Un método para co-compartimentar un polipéptido cíclico con un polinucleótido que codifica el polipéptido cíclico, que comprende los pasos de a) formar un compartimento que contiene un polinucleótido que codifica el polipéptido cíclico, b) expresar un polipéptido a partir del polinucleótido, y c) ciclar el polipéptido. Polipéptidos cíclicos co-compartimentados y polinucleótidos codificadores. Bibliotecas de polipéptidos cíclicos compartimentados y polinucleótidos codificantes. Métodos para el rastreo de bibliotecas de polipéptidos cíclicos cocompartimentados y polinucleótidos codificantes. Incorporación de ácidos nucleicos no canónicos en dichas bibliotecas.
MX2019010007A 2017-02-23 2018-02-22 Métodos para generar y separar por exclusión genotecas de péptidos compartimentados. MX2019010007A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1702938.0A GB201702938D0 (en) 2017-02-23 2017-02-23 Methods of generating and screening compartmentalised peptides libraries
PCT/EP2018/054443 WO2018154021A1 (en) 2017-02-23 2018-02-22 Methods for generating and screening compartmentalised peptide libraries

Publications (1)

Publication Number Publication Date
MX2019010007A true MX2019010007A (es) 2019-12-19

Family

ID=58544063

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019010007A MX2019010007A (es) 2017-02-23 2018-02-22 Métodos para generar y separar por exclusión genotecas de péptidos compartimentados.

Country Status (12)

Country Link
US (1) US20200049695A1 (es)
EP (1) EP3586134B1 (es)
JP (1) JP2020511432A (es)
KR (1) KR102567657B1 (es)
CN (1) CN110520729B (es)
AU (1) AU2018223899C1 (es)
CA (1) CA3054251A1 (es)
ES (1) ES2972599T3 (es)
GB (1) GB201702938D0 (es)
MX (1) MX2019010007A (es)
WO (1) WO2018154021A1 (es)
ZA (1) ZA201905538B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018165159A1 (en) * 2017-03-06 2018-09-13 Synvitrobio, Inc. Methods and systems for cell-free biodiscovery of natural products
WO2018225864A1 (ja) * 2017-06-09 2018-12-13 中外製薬株式会社 膜透過性の高い環状ペプチド化合物、及びこれを含むライブラリ
CN110218776A (zh) * 2019-06-14 2019-09-10 苏州叠代生物科技有限公司 Pcr油相组合物及其制备方法
CN114902024A (zh) * 2019-11-15 2022-08-12 闪璨迈璀公司 基于荧光团扩散的组合物和方法
WO2021174199A1 (en) * 2020-02-28 2021-09-02 Synvitrobio, Inc. Methods and compositions for identifying traits using a cell-free system

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2260956T3 (es) * 1998-12-18 2006-11-01 The Penn State Research Foundation Ciclacion de peptidos mediada por inteina.
WO2001014539A2 (en) * 1999-08-20 2001-03-01 Johns Hopkins University School Of Medicine Methods and compositions for the construction and use of fusion libraries
EP1691792A4 (en) * 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND METHODS FOR IN VITRO / I SORTING OF MOLECULAR AND CELLULAR BANKS
US7968287B2 (en) * 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
GB201108259D0 (en) * 2011-05-17 2011-06-29 Cambridge Entpr Ltd Gel beads in microfluidic droplets
JP6754997B2 (ja) * 2013-08-26 2020-09-16 国立大学法人 東京大学 大環状ペプチド、その製造方法、及び大環状ペプチドライブラリを用いるスクリーニング方法

Also Published As

Publication number Publication date
EP3586134C0 (en) 2023-12-13
AU2018223899C1 (en) 2024-03-28
US20200049695A1 (en) 2020-02-13
KR102567657B1 (ko) 2023-08-16
JP2020511432A (ja) 2020-04-16
KR20190137078A (ko) 2019-12-10
AU2018223899B2 (en) 2023-11-30
CA3054251A1 (en) 2018-08-30
ZA201905538B (en) 2024-01-31
EP3586134A1 (en) 2020-01-01
ES2972599T3 (es) 2024-06-13
WO2018154021A1 (en) 2018-08-30
CN110520729B (zh) 2024-05-28
GB201702938D0 (en) 2017-04-12
AU2018223899A1 (en) 2019-09-12
EP3586134B1 (en) 2023-12-13
CN110520729A (zh) 2019-11-29

Similar Documents

Publication Publication Date Title
ZA201905538B (en) Methods for generating and screening compartmentalised peptide libraries
MX2021013161A (es) Polipeptidos que contienen dominios de union de novo y usos de los mismos.
PH12019501413A1 (en) T-cell modulatory multimeric polypeptides and method of use thereof
PH12018501280A1 (en) Anti-myostatin antibodies, polypeptides containing variant fc regions, and methods of use
PH12017501115A1 (en) Anti-myostatin antibodies, polypeptides containing variant fc regions, and method of use
MX2023007211A (es) Anticuerpos antimiostatina y metodos de uso.
WO2017151818A3 (en) T-cell modulatory multimeric polypeptides and methods of use thereof
WO2016077052A3 (en) Evolution of proteases
MX2020008180A (es) Anticuerpos activables y métodos para elaborarlos y usarlos.
PH12020550240A1 (en) D-domain containing polypeptides and uses thereof
TN2017000309A1 (en) Contexts for large coding tree units
WO2015150900A3 (en) Fgfr fusions
EA201791564A1 (ru) Ограничение на размер палитрового блока при кодировании видео
EP4059961A3 (en) Anti-tau constructs
MX2017003578A (es) Sistemas, metodos y dispositivos para la fusion de atributos previstos de un camino y de historiales de conduccion.
IL277120A (en) Antimicrobial Peptide Lysine Polypeptide Templates, Lysines, Isolated Polynucleotides Encoding Them and Their Uses
WO2018076024A8 (en) Methods for screening b cell lymphocytes
CL2020001785A1 (es) Selección de transformación en un codificador de vídeo y / o un decodificador de vídeo.
MX2021000263A (es) Proteina de fusion variante sirpalfa-4-1bbl y procedimientos de uso de la misma.
WO2015166105A3 (en) Ion channel modulators and uses thereof
GB201720671D0 (en) The generating method of data authentication code
EP3567107A4 (en) GENE ASSOCIATED WITH THE SYNTHESIS OF A CYCLIC PEPTIDE COMPOUND, PRODUCTION METHOD FOR A CYCLIC PEPTIDE COMPOUND THEREOF AND TRANSFORMANTLY therewith
ZA202001389B (en) Non-human animals expressing humanized c1q complex
MX2021015766A (es) Nuevos antigenos y metodos contra el cancer.
MX2019009183A (es) Metodo y dispositivo para colocar una linea de llenado en un recipiente.