MX2015007201A - Genetic system for the production of leupeptin and its use for heterologous protein production. - Google Patents

Genetic system for the production of leupeptin and its use for heterologous protein production.

Info

Publication number
MX2015007201A
MX2015007201A MX2015007201A MX2015007201A MX2015007201A MX 2015007201 A MX2015007201 A MX 2015007201A MX 2015007201 A MX2015007201 A MX 2015007201A MX 2015007201 A MX2015007201 A MX 2015007201A MX 2015007201 A MX2015007201 A MX 2015007201A
Authority
MX
Mexico
Prior art keywords
leupeptin
production
genes
direct
producers
Prior art date
Application number
MX2015007201A
Other languages
Spanish (es)
Inventor
Cruz Morales Pablo
Barona Gómez Francisco
Eréndira Ramos Aboites Hilda
Antonio Morales Escalante Marco
Original Assignee
Centro De Investigación Y De Estudios Avanzados Del I P N
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro De Investigación Y De Estudios Avanzados Del I P N filed Critical Centro De Investigación Y De Estudios Avanzados Del I P N
Priority to MX2015007201A priority Critical patent/MX2015007201A/en
Priority to PCT/IB2016/053295 priority patent/WO2016193954A2/en
Publication of MX2015007201A publication Critical patent/MX2015007201A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/0808—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms, e.g. Val, Ile, Leu
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention describes the discovery of the genes that direct the biosynthetic pathway of leupeptin, a potent protease inhibitor of the type of small peptide aldehyde (SPA). The prediction of genes that direct the biosynthesis of leupeptin was performed by EvoMining, a bioinformatic pipe for genome data mining of natural products based on evolutionary parameters and was confirmed by molecular biology techniques such as insertional mutagenesis and the comparison of metabolomic profiles between wild strains (producers of leupeptin) and mutants (non producers of leupeptin). Biosynthetic genes of leupeptin were cloned and expressed heterologously in other expression systems of bacterial origin, thus achieving the production of leupeptin. It also describes the design of a cassette for heterologous production of leupeptin and its use for producing leupeptin and variants of it using synthetic biology, and to produce recombinant proteins.
MX2015007201A 2015-06-05 2015-06-05 Genetic system for the production of leupeptin and its use for heterologous protein production. MX2015007201A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MX2015007201A MX2015007201A (en) 2015-06-05 2015-06-05 Genetic system for the production of leupeptin and its use for heterologous protein production.
PCT/IB2016/053295 WO2016193954A2 (en) 2015-06-05 2016-06-05 Genetic system for the production of leupeptin and its use for heterologous protein production

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX2015007201A MX2015007201A (en) 2015-06-05 2015-06-05 Genetic system for the production of leupeptin and its use for heterologous protein production.

Publications (1)

Publication Number Publication Date
MX2015007201A true MX2015007201A (en) 2016-12-05

Family

ID=57440359

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015007201A MX2015007201A (en) 2015-06-05 2015-06-05 Genetic system for the production of leupeptin and its use for heterologous protein production.

Country Status (2)

Country Link
MX (1) MX2015007201A (en)
WO (1) WO2016193954A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108893481B (en) * 2018-06-21 2021-07-02 山西大学 Tomato SlOAS7 gene and application thereof
US11912742B2 (en) * 2020-07-01 2024-02-27 Yale University Heterologous production of leupeptin protease inhibitors and analogs thereof
MX2021012945A (en) * 2021-10-22 2023-04-24 Centro De Investig Y De Estudios Avanzados Del I P N Genetic system for leupeptin biosynthesis and its use in heterologous production systems.

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1477563A3 (en) * 2003-05-16 2004-11-24 Wyeth Cloning genes from streptomyces cyaneogriseus subsp.noncyanogenus for biosynthesis of antibiotics and methods of use

Also Published As

Publication number Publication date
WO2016193954A2 (en) 2016-12-08
WO2016193954A3 (en) 2017-01-26

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