MX2015001503A - System and culture process of synchronized cells in bioreactors. - Google Patents

System and culture process of synchronized cells in bioreactors.

Info

Publication number
MX2015001503A
MX2015001503A MX2015001503A MX2015001503A MX2015001503A MX 2015001503 A MX2015001503 A MX 2015001503A MX 2015001503 A MX2015001503 A MX 2015001503A MX 2015001503 A MX2015001503 A MX 2015001503A MX 2015001503 A MX2015001503 A MX 2015001503A
Authority
MX
Mexico
Prior art keywords
cell
bioreactors
synchronized
production
physiology
Prior art date
Application number
MX2015001503A
Other languages
Spanish (es)
Inventor
Zoila Vanessa Hernandez Rodriguez
Jose Antonio Serrato Perez
Jocelyne Elena Mendoza Perez
Original Assignee
Univ Mexico Nacional Autonoma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Mexico Nacional Autonoma filed Critical Univ Mexico Nacional Autonoma
Priority to MX2015001503A priority Critical patent/MX2015001503A/en
Publication of MX2015001503A publication Critical patent/MX2015001503A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The field of the present disclosure is related to the cell culture technology. The invention is related to a system and method for improving the culture conditions of animal cells for the study of metabolism and cell physiology, as well as for the production of recombinant proteins of commercial interest. The present invention discloses a cellular culture system and method in mini-bioreactors to perform synchronized cell cultures under controlled conditions, predetermined and with sufficient cell concentrations for detailed kinetic studies of the physiology and cellular metabolism and for the production of recombinant proteins by means of synchronized cell cultures. The invention consists of: a cell population separation system based on the density and cell size for generating a synchronized population; a cell injection system to minimize handling and cell damage; and two cell culture systems in mini-bioreactors implemented for performing cultures under controlled and predetermined co nditions. The scope of the present invention extends to the production of metabolism and cell physiology products as well as the selection and optimization of cell lines through this system and/or method.
MX2015001503A 2015-01-30 2015-01-30 System and culture process of synchronized cells in bioreactors. MX2015001503A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MX2015001503A MX2015001503A (en) 2015-01-30 2015-01-30 System and culture process of synchronized cells in bioreactors.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX2015001503A MX2015001503A (en) 2015-01-30 2015-01-30 System and culture process of synchronized cells in bioreactors.

Publications (1)

Publication Number Publication Date
MX2015001503A true MX2015001503A (en) 2016-07-29

Family

ID=57219149

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015001503A MX2015001503A (en) 2015-01-30 2015-01-30 System and culture process of synchronized cells in bioreactors.

Country Status (1)

Country Link
MX (1) MX2015001503A (en)

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