MX2013012709A - Estrategia de analisis de arnm bacteriano para descubrir nuevas moleculas de acido nucleico codificante de pesticidas. - Google Patents
Estrategia de analisis de arnm bacteriano para descubrir nuevas moleculas de acido nucleico codificante de pesticidas.Info
- Publication number
- MX2013012709A MX2013012709A MX2013012709A MX2013012709A MX2013012709A MX 2013012709 A MX2013012709 A MX 2013012709A MX 2013012709 A MX2013012709 A MX 2013012709A MX 2013012709 A MX2013012709 A MX 2013012709A MX 2013012709 A MX2013012709 A MX 2013012709A
- Authority
- MX
- Mexico
- Prior art keywords
- nucleic acid
- encoding nucleic
- acid molecule
- pesticide
- encoding
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Se proporciona métodos para aislar e identificar nuevas moléculas de ácidos nucleicos codificantes de pesticidas a partir de las cepas bacterianas que se identificó que tienen un polipéptido pesticida. Los métodos incluyen seleccionar una cepa bacteriana que tiene o que se sospecha que tiene por lo menos un plásmido codificante de pesticidas, curar el por lo menos único plásmido codificante de pesticidas de la cepa bacteriana y realizar una hibridación sustractiva para obtener ARNm plasmídico que codifica el polipéptido pesticida. El ARNm plasmídico puede usarse para producir una genoteca de ADNc de la cual se puede aislar y posteriormente identificar el polipéptido pesticida.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161481442P | 2011-05-02 | 2011-05-02 | |
PCT/US2012/035740 WO2012151145A1 (en) | 2011-05-02 | 2012-04-30 | Bacterial mrna screen strategy for novel pesticide-encoding nucleic acid molecule discovery |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2013012709A true MX2013012709A (es) | 2013-12-06 |
Family
ID=46051949
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2013012709A MX2013012709A (es) | 2011-05-02 | 2012-04-30 | Estrategia de analisis de arnm bacteriano para descubrir nuevas moleculas de acido nucleico codificante de pesticidas. |
Country Status (7)
Country | Link |
---|---|
US (1) | US20120283111A1 (es) |
EP (1) | EP2705153A1 (es) |
CN (1) | CN103502457A (es) |
BR (1) | BR112013028318A2 (es) |
CA (1) | CA2834364A1 (es) |
MX (1) | MX2013012709A (es) |
WO (1) | WO2012151145A1 (es) |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8526774D0 (en) | 1985-10-30 | 1985-12-04 | Sandoz Ltd | Bacillus thuringiensis hybrids |
US5310652A (en) | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
US5407800A (en) | 1986-08-22 | 1995-04-18 | Hoffmann-La Roche Inc. | Reverse transcription with Thermus thermophilus polymerase |
US5080897A (en) * | 1987-05-08 | 1992-01-14 | Ecogen Inc. | Novel bacillus thuringiensis strains, and related insecticidal compositions |
GB8910624D0 (en) | 1989-05-09 | 1989-06-21 | Ici Plc | Bacterial strains |
US5268289A (en) | 1991-12-27 | 1993-12-07 | Epicentre Technologies Corp. | Thermostable ribonuclease H |
US6268181B1 (en) | 1992-11-05 | 2001-07-31 | Valent Biosciences, Corporation | Methods for producing a potentiator of Bacillus pesticidal activity |
US5436142A (en) | 1992-11-12 | 1995-07-25 | Cold Spring Harbor Laboratory | Methods for producing probes capable of distingushing variant genomic sequences |
US5351643A (en) | 1992-12-11 | 1994-10-04 | Boyce Thompson Institute For Plant Research, Inc. | High density rearing system for larvae |
JP2879303B2 (ja) | 1993-01-14 | 1999-04-05 | 佑 本庶 | cDNAライブラリーの作製方法、および新規なポリペプチドとそれをコードするDNA |
US5849870A (en) | 1993-03-25 | 1998-12-15 | Novartis Finance Corporation | Pesticidal proteins and strains |
US5591575A (en) | 1993-04-07 | 1997-01-07 | Amersham International Plc | Subtraction hybridization employing aziridinylbenoquinone cross-linking agents |
US5512468A (en) | 1993-11-12 | 1996-04-30 | Stratagene | Process of producing highly transformable bacterial cells and cells produced thereby |
US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
US5935788A (en) | 1996-06-25 | 1999-08-10 | Lifespan Biosciences, Inc. | Subtractive hybridization techniques for identifying differentially expressed and commonly expressed nucleic acid |
US6570005B1 (en) | 1996-07-01 | 2003-05-27 | Mycogen Corporation | Toxins active against pests |
US6030814A (en) | 1997-04-21 | 2000-02-29 | Epicentre Technologies Corporation | Reverse transcription method |
US6057491A (en) | 1997-05-29 | 2000-05-02 | Borad Of Regents For University Of Oklahoma | Protein having insecticidal activities and method of use |
EP0991775A1 (en) | 1997-06-20 | 2000-04-12 | Mycogen Corporation | Method to identify pesticidal microbes |
US6638717B2 (en) | 1999-05-19 | 2003-10-28 | Aventis Pharmaceuticals, Inc. | Microarray-based subtractive hybridzation |
-
2012
- 2012-04-30 BR BR112013028318A patent/BR112013028318A2/pt not_active IP Right Cessation
- 2012-04-30 CN CN201280021349.2A patent/CN103502457A/zh active Pending
- 2012-04-30 EP EP12720059.0A patent/EP2705153A1/en not_active Withdrawn
- 2012-04-30 WO PCT/US2012/035740 patent/WO2012151145A1/en active Application Filing
- 2012-04-30 MX MX2013012709A patent/MX2013012709A/es not_active Application Discontinuation
- 2012-04-30 CA CA2834364A patent/CA2834364A1/en not_active Abandoned
- 2012-04-30 US US13/459,280 patent/US20120283111A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
BR112013028318A2 (pt) | 2017-01-10 |
EP2705153A1 (en) | 2014-03-12 |
US20120283111A1 (en) | 2012-11-08 |
WO2012151145A1 (en) | 2012-11-08 |
CA2834364A1 (en) | 2012-11-08 |
CN103502457A (zh) | 2014-01-08 |
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Legal Events
Date | Code | Title | Description |
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FA | Abandonment or withdrawal |