MX2012000297A - Cofactores, donadores y aceptadores de ligasa quimicamente modificados. - Google Patents
Cofactores, donadores y aceptadores de ligasa quimicamente modificados.Info
- Publication number
- MX2012000297A MX2012000297A MX2012000297A MX2012000297A MX2012000297A MX 2012000297 A MX2012000297 A MX 2012000297A MX 2012000297 A MX2012000297 A MX 2012000297A MX 2012000297 A MX2012000297 A MX 2012000297A MX 2012000297 A MX2012000297 A MX 2012000297A
- Authority
- MX
- Mexico
- Prior art keywords
- modified
- ligation
- acceptors
- donors
- chemically modified
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
En este documento se proporcionan métodos para la ligación de polinucleótidos que contienen componentes modificados de ligación, particularmente cofactores modificados, aceptores modificados y donadores modificados de ligasa. Los métodos se aplican fácilmente a los ensayos basados en ligación para la detección de una secuencia de ácido nucleico donde el uso del cofactor modificado mejora la discriminación entre moldes coincidentes y sin coincidencia. Además, el uso de los componentes modificados de ligación reduce o elimina la ligación en ausencia del molde de ácido nucleico.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22336409P | 2009-07-06 | 2009-07-06 | |
PCT/US2010/041069 WO2011005762A1 (en) | 2009-07-06 | 2010-07-06 | Chemically modified ligase cofactors, donors and acceptors |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2012000297A true MX2012000297A (es) | 2012-04-30 |
Family
ID=43427762
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2012000297A MX2012000297A (es) | 2009-07-06 | 2010-07-06 | Cofactores, donadores y aceptadores de ligasa quimicamente modificados. |
Country Status (9)
Country | Link |
---|---|
US (1) | US8728725B2 (es) |
EP (1) | EP2451980B1 (es) |
JP (1) | JP5903379B2 (es) |
AU (1) | AU2010270715C1 (es) |
CA (1) | CA2767408C (es) |
ES (1) | ES2521740T3 (es) |
MX (1) | MX2012000297A (es) |
NZ (1) | NZ597535A (es) |
WO (1) | WO2011005762A1 (es) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AP3396A (en) | 2009-02-13 | 2015-08-31 | Chem Inc X | Methods of creating and screening DNA-encoded libraries |
US9631227B2 (en) * | 2009-07-06 | 2017-04-25 | Trilink Biotechnologies, Inc. | Chemically modified ligase cofactors, donors and acceptors |
EP2665833B1 (en) * | 2011-01-17 | 2017-04-19 | Life Technologies Corporation | Workflow for detection of ligands using nucleic acids |
EP2665815B1 (en) | 2011-01-17 | 2017-08-09 | Life Technologies Corporation | Enzymatic ligation of nucleic acids |
SG11201400374TA (en) | 2011-09-07 | 2014-09-26 | Chem Inc X | Methods for tagging dna-encoded libraries |
US9403863B2 (en) | 2011-09-12 | 2016-08-02 | Idenix Pharmaceuticals Llc | Substituted carbonyloxymethylphosphoramidate compounds and pharmaceutical compositions for the treatment of viral infections |
US9109001B2 (en) | 2012-05-22 | 2015-08-18 | Idenix Pharmaceuticals, Inc. | 3′,5′-cyclic phosphoramidate prodrugs for HCV infection |
AU2013266393B2 (en) | 2012-05-22 | 2017-09-28 | Idenix Pharmaceuticals Llc | D-amino acid compounds for liver disease |
WO2013177195A1 (en) | 2012-05-22 | 2013-11-28 | Idenix Pharmaceuticals, Inc. | 3',5'-cyclic phosphate prodrugs for hcv infection |
EA027929B1 (ru) | 2012-05-25 | 2017-09-29 | Янссен Сайенсиз Айрлэнд Юси | Нуклеозиды на основе урацила и спирооксетана |
EP2900682A1 (en) | 2012-09-27 | 2015-08-05 | IDENIX Pharmaceuticals, Inc. | Esters and malonates of sate prodrugs |
US10513534B2 (en) | 2012-10-08 | 2019-12-24 | Idenix Pharmaceuticals Llc | 2′-chloro nucleoside analogs for HCV infection |
EP2935304A1 (en) | 2012-12-19 | 2015-10-28 | IDENIX Pharmaceuticals, Inc. | 4'-fluoro nucleosides for the treatment of hcv |
EP2970358B1 (en) | 2013-03-04 | 2021-06-30 | Idenix Pharmaceuticals LLC | 3'-deoxy nucleosides for the treatment of hcv |
WO2014137930A1 (en) | 2013-03-04 | 2014-09-12 | Idenix Pharmaceuticals, Inc. | Thiophosphate nucleosides for the treatment of hcv |
EP2970357A1 (en) | 2013-03-13 | 2016-01-20 | IDENIX Pharmaceuticals, Inc. | Amino acid phosphoramidate pronucleotides of 2'-cyano, azido and amino nucleosides for the treatment of hcv |
WO2014165542A1 (en) | 2013-04-01 | 2014-10-09 | Idenix Pharmaceuticals, Inc. | 2',4'-fluoro nucleosides for the treatment of hcv |
WO2014197578A1 (en) | 2013-06-05 | 2014-12-11 | Idenix Pharmaceuticals, Inc. | 1',4'-thio nucleosides for the treatment of hcv |
WO2015017713A1 (en) | 2013-08-01 | 2015-02-05 | Idenix Pharmaceuticals, Inc. | D-amino acid phosphoramidate pronucleotides of halogeno pyrimidine compounds for liver disease |
EP3131914B1 (en) | 2014-04-16 | 2023-05-10 | Idenix Pharmaceuticals LLC | 3'-substituted methyl or alkynyl nucleosides for the treatment of hcv |
CN106591425A (zh) * | 2015-10-15 | 2017-04-26 | 北京寻因生物科技有限公司 | 基于连接反应的多重靶向检测核酸指标的方法 |
CN106916877A (zh) * | 2015-12-24 | 2017-07-04 | 广州好芝生物科技有限公司 | 一种结核分枝杆菌利福平耐药突变检测试剂盒 |
CN108179180A (zh) * | 2017-10-25 | 2018-06-19 | 广州和康医疗技术有限公司 | 一种对cyp3a5*3位点进行基因型检测的方法和试剂盒 |
WO2023280156A1 (zh) * | 2021-07-08 | 2023-01-12 | 深圳华大生命科学研究院 | 修饰的核苷或核苷酸 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5593826A (en) * | 1993-03-22 | 1997-01-14 | Perkin-Elmer Corporation, Applied Biosystems, Inc. | Enzymatic ligation of 3'amino-substituted oligonucleotides |
US5508179A (en) * | 1994-03-18 | 1996-04-16 | Bio-Rad Laboratories, Inc. | Use of deoxyribose nicotinamide adenine dinucleotide to enhance the specificity of NAD+ -dependent ligation reactions |
US6312892B1 (en) * | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
CA2370478A1 (en) * | 1999-03-24 | 2000-09-28 | Serge L. Beaucage | N-acylphosphoramidites and their use in oligonucleotide synthesis |
US6297016B1 (en) * | 1999-10-08 | 2001-10-02 | Applera Corporation | Template-dependent ligation with PNA-DNA chimeric probes |
US6635425B2 (en) * | 2001-01-05 | 2003-10-21 | Molecular Staging, Inc. | 5′-thio phosphate directed ligation of oligonucleotides and use in detection of single nucleotide polymorphisms |
US8808991B2 (en) * | 2003-09-02 | 2014-08-19 | Keygene N.V. | Ola-based methods for the detection of target nucleic avid sequences |
WO2012094343A1 (en) | 2011-01-05 | 2012-07-12 | Trilink Biotechnologies | Chemically substituted thermosensitive probes and cofactors for hot start ligation |
-
2010
- 2010-07-06 CA CA2767408A patent/CA2767408C/en active Active
- 2010-07-06 ES ES10797720.9T patent/ES2521740T3/es active Active
- 2010-07-06 EP EP10797720.9A patent/EP2451980B1/en active Active
- 2010-07-06 AU AU2010270715A patent/AU2010270715C1/en active Active
- 2010-07-06 WO PCT/US2010/041069 patent/WO2011005762A1/en active Application Filing
- 2010-07-06 US US12/831,212 patent/US8728725B2/en active Active
- 2010-07-06 NZ NZ597535A patent/NZ597535A/en unknown
- 2010-07-06 JP JP2012519669A patent/JP5903379B2/ja active Active
- 2010-07-06 MX MX2012000297A patent/MX2012000297A/es active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
US20110008788A1 (en) | 2011-01-13 |
JP5903379B2 (ja) | 2016-04-13 |
AU2010270715A1 (en) | 2012-02-02 |
CA2767408C (en) | 2018-01-23 |
AU2010270715C1 (en) | 2016-03-17 |
JP2012531928A (ja) | 2012-12-13 |
EP2451980A4 (en) | 2012-10-31 |
AU2010270715B2 (en) | 2015-06-25 |
EP2451980A1 (en) | 2012-05-16 |
US8728725B2 (en) | 2014-05-20 |
CA2767408A1 (en) | 2011-01-13 |
ES2521740T3 (es) | 2014-11-13 |
WO2011005762A1 (en) | 2011-01-13 |
NZ597535A (en) | 2013-12-20 |
EP2451980B1 (en) | 2014-05-21 |
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